A disuse osteoporosis mouse experimental device and experimental method

Abstract

The present invention provides a disuse osteoporosis mouse experimental device and experimental method. The device includes a support member, the middle part of which is provided with a first through hole for lifting the mouse's hip and abdomen to make the mouse's hind limbs leave the crawling surface; the size of the first through hole is adapted to the size of the mouse's hip and abdomen; the upper part of the support member is fixedly connected to the mouse's tail. The experimental device and experimental method provided by the present invention can not only achieve the disuse of the mouse's hind limbs, but also avoid trauma to the mouse, thereby achieving a good disuse osteoporosis experimental effect; in addition, the experimental device provided by the present invention also has the advantages of low cost, small size, and easy expansion.

Description

Technical Field

[0001] The invention relates to the technical field of animal experimental devices, in particular to an experimental device and an experimental method for disuse osteoporosis mice. Background Art

[0002] Disuse osteoporosis is osteoporosis caused by a decrease in bone mineral content due to limited mobility or functional impairment.

[0003] At present, animal models and methods of disuse osteoporosis mainly include: tail suspension, leg and tail fixation, hind limb plaster splint fixation, sciatic nerve transection and muscle and tendon resection, etc. Among them, tail suspension is the most commonly used method. Most of the tail suspension methods use rats as experimental subjects. However, the bone cortical reconstruction activity of rats is low, which makes it difficult to apply to the research related to bone Harvard system reconstruction. However, when the tail suspension method is applied to mice, after a certain period of suspension, due to the obstruction of blood circulation in the tail of mice, tissue ulceration and tail fracture will occur, so long-term modeling cannot be achieved. In addition, since the modeling process of the tail suspension method is relatively complicated, a specific roller or spring device is required so that the mice can move to eat and drink water, and the requirements for the feeding device are relatively high. At the same time, the space utilization rate of the feeding cage is low, and more feeding cages are required, which increases the workload of feeding and causes inconvenience to scientific experiments. In addition, other animal models and methods of disuse osteoporosis, such as tail fixation and sciatic nerve transection, can limit the movement of the hind limbs, but they have disadvantages such as complexity, invasiveness, and susceptibility to infection and inflammation. Another example is the hind limb plaster splint fixation method, which requires frequent replacement of the plaster splint, and the procedure is relatively complicated. In addition, the fixed hind limbs are still partially on the ground under stress, resulting in less than ideal modeling effects.

[0004] Therefore, the existing animal models and methods for disuse osteoporosis have defects such as high requirements for experimental equipment, low equipment utilization, invasive modeling methods, complicated procedures, and low efficiency. Summary of the invention

[0005] In view of the above-mentioned shortcomings of the prior art, the purpose of the present invention is to provide a disuse osteoporosis mouse experimental device and experimental method to solve the defects of the existing experimental device, such as high requirements, low device utilization rate, traumatic experimental method, complicated procedures and low efficiency.

[0006] In order to achieve the above-mentioned purpose and other related purposes, the present invention first provides a disuse osteoporosis mouse experimental device, which includes a support member, wherein the middle part of the support member is provided with a first through hole for supporting the mouse's hip and abdomen so that the mouse's hind limbs are separated from the crawling surface; the size of the first through hole is adapted to the size of the mouse's hip and abdomen; and the upper part of the support member is fixedly connected to the mouse's tail. According to the disuse osteoporosis mouse experimental device provided by the present invention, after placing the mouse in the first through hole of the support member and fixing the mouse's tail relative to the support member, the mouse's hind limbs can be kept away from the crawling surface, achieving the disuse of the hind limbs without affecting the normal activities of the mouse's forelimbs, and without interfering with the mouse's water collection and food intake, thereby avoiding trauma to the mouse.

[0007] In addition, the disuse osteoporosis mouse experimental device provided by the present invention also has the following characteristics:

[0008] In one embodiment of the present invention, the height of the center point of the first through hole on the longitudinal section of the support member is 0.27 to 0.84 times the distance between the mouse hip and abdomen and the mouse head.

[0009] In one embodiment of the present invention, the thickness of the support member is 1 / 8 to 1 / 6 of the body length of the mouse.

[0010] In one embodiment of the present invention, the disuse osteoporosis mouse experimental device also includes at least one set of first extrusion members and a first adjustment unit configured to adjust the size of the first through hole; wherein the first extrusion member is disposed in the first through hole and is connected to the support member by connecting the first adjustment unit.

[0011] In one embodiment of the present invention, a single group of the first adjustment unit includes a first threaded rod and a first rotating member; a first threaded through hole adapted to the first threaded rod is provided in the support member, and the first threaded through hole is threadedly connected to the first threaded rod; and one end of the first threaded rod is connected to the first rotating member, and the other end is connected to the first extrusion member.

[0012] In one embodiment of the present invention, a first support seat is provided on the outer side of the support member at the connection between the first threaded rod and the first threaded through hole and is sleeved on the outer side of the thread of the first threaded rod.

[0013] In one embodiment of the present invention, the upper portion of the support member is provided with a second through hole for passing and fixing the mouse's tail.

[0014] In one embodiment of the present invention, the disuse osteoporosis mouse experimental device also includes at least one set of second extrusion members and second adjustment units configured to adjust the size of the second through hole; wherein the second extrusion member is disposed in the second through hole and is connected to the support member by connecting the second adjustment unit.

[0015] In one embodiment of the present invention, a single group of the second adjustment unit includes a second threaded rod and a second rotating member; a second threaded through hole adapted to the second threaded rod is provided in the support member, and the second threaded through hole is threadedly connected to the second threaded rod; and one end of the second threaded rod is connected to the second rotating member, and the other end is connected to the second extrusion member.

[0016] In one embodiment of the present invention, a second support seat is provided on the outer side of the support member at the connection between the second threaded rod and the second threaded through hole and is sleeved on the outer side of the thread of the second threaded rod.

[0017] In one embodiment of the present invention, the material of the support member includes polystyrene material.

[0018] In one embodiment of the present invention, a roller is disposed at the bottom of the support member.

[0019] In addition, the present invention also provides an experimental method of a disuse osteoporosis mouse experimental device, the experimental method comprising the following steps:

[0020] Obtaining the body parameters of the mouse, including the size of the mouse's hip and abdomen, the distance from the mouse's head to the hip and abdomen, and the mouse's body length; constructing the disuse osteoporosis mouse experimental device based on the mouse's body parameters;

[0021] Installing the disuse osteoporosis mouse experimental device on the hip and abdomen of the mouse;

[0022] The physiological indexes of the femur of the mouse were detected.

[0023] As described above, the experimental device and method for disuse osteoporosis in mice provided by the present invention can not only achieve the disuse of the mice's hind limbs, but also avoid trauma to the mice, thereby achieving good disuse osteoporosis experimental results. The adjustment unit and the extrusion structure can also be used to make the experimental device adaptable to mice of different sizes, thereby improving ease of use and expanding the scope of application. In addition, the experimental device provided by the present invention also has the advantages of low cost, small size, and easy expansion. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1A Shown is a schematic structural diagram of the disuse osteoporosis mouse experimental device provided by the present invention in Example 1;

[0025] Figure 1B Shown is a left view of the disuse osteoporosis mouse experimental device provided by the present invention in Example 1;

[0026] Figure 2 The figure shows the state of the disuse osteoporosis mouse experimental device in Example 1 being fixed to the mouse hip abdomen;

[0027] Figure 3 Shown is a schematic diagram of the structure of Example 2 of the disuse osteoporosis mouse experimental device provided by the present invention;

[0028] Figure 4 The figure shows the state of the disuse osteoporosis mouse experimental device in Example 2 being fixed to the mouse hip abdomen;

[0029] Figure 5 Shown is a schematic diagram of the process of the disuse osteoporosis mouse experimental method provided by the present invention in Example 3;

[0030] Figure 6 The figure shows the condition of the hind limbs of mice after one month of modeling using the disuse osteoporosis mouse experimental device described in Example 1;

[0031] Figure 7 The results and quantitative statistics of new bone formation in the femur of mice after one month of modeling using the disuse osteoporosis mouse experimental device described in Example 1 are shown;

[0032] Figure 8 The display shows the bone absorption test results and quantitative statistics of the femur of mice after one month of modeling using the disuse osteoporosis mouse experimental device described in Example 1;

[0033] Fig. 9 The display shows the test results and quantitative statistics of rat cancellous bone after one month of modeling using the disuse osteoporosis rat experimental device described in Example 1;

[0034] Fig.10 Shown are the cortical bone test results and quantitative statistics of mice one month after modeling using the disuse osteoporosis mouse experimental device described in Example 1.

[0035] Component number description

[0036] Support 1

[0037] First through hole 2

[0038] First extrusion 3

[0039] First adjustment unit 4

[0040] First threaded rod 41

[0041] The first rotating member 42

[0042] First support seat 5

[0043] Second through hole 6

[0044] Second extruded part 7

[0045] Second adjustment unit 8

[0046] Second threaded rod 81

[0047] The second rotating member 82

[0048] The second support seat 9

[0049] Roller 10 DETAILED DESCRIPTION

[0050] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the following embodiments and features in the embodiments can be combined with each other without conflict.

[0051] It should be noted that the illustrations provided in the following embodiments are only schematic illustrations of the basic concept of the present invention, and thus the drawings only show components related to the present invention rather than being drawn according to the number, shape and size of components in actual implementation. In actual implementation, the type, quantity and proportion of each component may be changed arbitrarily, and the component layout may also be more complicated.

[0052] Example 1

[0053] See also Figure 1A , Figure 1B and Figure 2 , respectively, are a schematic diagram of the structure of the disuse osteoporosis mouse experimental device provided in Example 1 of the present invention, and a state diagram of the disuse osteoporosis mouse experimental device fixed to the mouse hip and abdomen.

[0054] like Figure 1A and Figure 1B As shown, the disuse osteoporosis mouse experimental device includes a support member 1; a first through hole 2 is provided in the middle of the support member 1 for supporting the mouse's hip and abdomen to allow the mouse's hind limbs to leave the crawling surface; the size of the first through hole is adapted to the size of the mouse's hip and abdomen.

[0055] The mouse hip and abdomen region includes the part where the trunk and hind limbs are connected and the lower abdomen.

[0056] The size of the first through hole is adapted to the size of the mouse's hip and abdomen, including the width and height of the first through hole in the cross section of the first through hole, which correspond to the body width and thickness of the mouse's hip and abdomen.

[0057] Preferably, the cross section of the first through hole ( Figure 1A The plane shown in the figure is elliptical; and in the cross section, the horizontal axial width of the first through hole is 0 to 2 mm larger than the body width of the mouse's hip and abdomen, and the vertical axial height of the first through hole is 0 to 2 mm larger than the body thickness of the mouse's hip and abdomen.

[0058] In this embodiment, the height of the center point of the first through hole on the longitudinal section of the support member is 0.27 to 0.84 times the distance between the mouse's hip and abdomen and the mouse's head, so that when the mouse is placed in the first through hole, the support member lifts the mouse's hip and abdomen to lift the mouse's hind legs off the ground; and the mouse's forward tilt angle is between 15° and 40°.

[0059] The height of the center point of the first through hole on the longitudinal section of the support member is the distance between the center point of the first through hole and the lowest point of the disuse osteoporosis mouse experimental device on the longitudinal section of the support member.

[0060] The forward tilt angle of the mouse is the angle between the line between the head and the hip and abdomen of the mouse and the crawling surface of the mouse.

[0061] In this embodiment, the thickness of the support member is 1 / 8 to 1 / 6 of the mouse's body length. While enabling the mouse's forelimbs to crawl freely, the support member ensures that the supporting area of ​​the mouse's hip and abdomen is of moderate size, avoiding affecting the mouse's flexibility due to excessive support area, and avoiding excessive pressure on the mouse's abdominal support due to too small support area, which may damage the mouse's abdominal organs and cause pathological changes.

[0062] The body length of a mouse is the distance from the head to the base of the tail.

[0063] The upper part of the support member is connected to the tail of the mouse, so that the support member can be better fixed to the hip and abdomen of the mouse to prevent the mouse from breaking free.

[0064] In a specific embodiment, the upper end of the support member is adhesively connected to the mouse tail. For example, a medical breathable tape is used to adhesively fix the mouse tail to the upper end of the support member.

[0065] Preferably, the support member is made of a lightweight material; in a specific implementation, the material of the support member includes polystyrene material.

[0066] The disuse osteoporosis mouse experimental device in this embodiment achieves the effect of lifting the mouse's hip and abdomen and lifting the mouse's hind limbs off the ground by fixing the support member with the first through hole on the mouse's hip and abdomen, and the characteristics of the support member are adapted to the mouse's body structure characteristics.

[0067] Example 2

[0068] See also Figure 3 and Figure 4 , respectively, are a schematic diagram of the structure of the disuse osteoporosis mouse experimental device provided in Implementation 2 of the present invention, and a state diagram of the disuse osteoporosis mouse experimental device fixed to the mouse hip and abdomen.

[0069] like Figure 3 As shown, based on the disuse osteoporosis mouse experimental device described in implementation 1, the device also includes at least one group of first extrusion members 3 and first adjustment units 4 configured to adjust the size of the first through hole; wherein the first extrusion member 3 is disposed in the first through hole 2, and is connected to the support member 1 by connecting to the first adjustment unit 4; and by adjusting the first adjustment unit 4, the position of the first adjustment member in the first through hole 2 is adjusted to achieve adjustment of the size of the first through hole 2.

[0070] Specifically, the first extrusion member is preferably made of a flexible material to avoid causing trauma to the mouse's hip and abdomen when the mouse is fixed.

[0071] Furthermore, the first extrusion member is in an inwardly concave arc shape on the side contacting the mouse's hip and abdomen, so that the first extrusion member can better adapt to the shape of the mouse's hip and abdomen, so as to better support and fix the mouse, as well as fix the relative position between the support member and the mouse.

[0072] In a specific embodiment, a single set of the first adjustment unit 4 includes a first threaded rod 41 and a first rotating member 42; the support member 1 is provided with a first threaded through hole connecting the outer side of the support member 1 and the first through hole 2, and the first threaded through hole is adapted to the first threaded rod 41; the first threaded through hole is threadedly connected to the first threaded rod 41; one end of the first threaded rod 41 is connected to the first adjustment member, and the other end is connected to the first extrusion member 3. By rotating the first rotating member, the first threaded rod is driven to rotate in the first threaded through hole to change the position of the first extrusion member in the first through hole, thereby adjusting the size of the first through hole so that the first through hole can better fit mice of different body sizes.

[0073] Furthermore, a first support seat is provided on the outer side of the support member at the connection between the first threaded rod and the first threaded through hole, and is sleeved on the outer side of the thread of the first threaded rod to fix and support the first threaded rod.

[0074] Preferably, the first support seat is threadedly connected to the first threaded rod.

[0075] In this embodiment, a second through hole 6 for passing and fixing the mouse's tail is provided on the upper portion of the support member 1. The size of the second through hole 6 is adapted to the cross-sectional size of the mouse's tail to better fix the relative position between the mouse's tail and the support member 1.

[0076] Furthermore, the disuse osteoporosis mouse experimental device also includes at least one group of the second extrusion member 7 and the second adjustment unit 8 configured to adjust the second through hole 6; wherein the second extrusion member 7 is located in the second through hole 6 and is connected to the support member 1 by connecting the second adjustment unit 8; and by adjusting the second adjustment unit, the position of the second extrusion member in the second through hole is adjusted to achieve adjustment of the size of the second through hole.

[0077] Specifically, the material and shape characteristics of the second extrusion component are the same as those of the first extrusion component, and are not described in detail herein.

[0078] In a specific embodiment, a single set of the second adjustment unit 8 includes a second threaded rod 81 and a second rotating member 82; the support member 1 is provided with a second threaded through hole connecting the outer side of the support member 1 and the second through hole 6, and the second threaded through hole is adapted to the second threaded rod 81; the second threaded through hole is threadedly connected to the second threaded rod 81; one end of the second threaded rod 81 is connected to the second rotating member 82, and the other end is connected to the second extrusion member 7. By rotating the second rotating member, the second threaded rod is driven to rotate in the second threaded through hole to change the position of the first extrusion member in the first through hole, thereby adjusting the size of the first through hole so that the first through hole can better fit mice of different body sizes.

[0079] Furthermore, a second support seat is provided on the outer side of the support member at the connection between the second threaded rod and the second threaded through hole, and is sleeved on the outer side of the thread of the second threaded rod for fixedly supporting the second threaded rod.

[0080] Preferably, the second support seat is threadedly connected to the second threaded rod.

[0081] Furthermore, the disuse osteoporosis mouse experimental device is provided with at least one set of rollers 10 at the bottom of the support member to reduce the friction between the support member and the mouse crawling surface, which is more conducive to the normal activities of the mouse.

[0082] The disuse osteoporosis mouse experimental device in this embodiment is Figure 1A and Figure 1B On the basis of what is shown, a second through hole for fixing the mouse's tail, and an extrusion piece and an adjustment unit for adjusting the size of the first through hole / the first through hole and the second through hole are added, so that the experimental device can better adapt to mice of different sizes, thereby improving the applicability of the device.

[0083] Example 3

[0084] In this embodiment, based on Figure 1A , Figure 1B and Figure 2 The disuse osteoporosis mouse experimental device shown provides a disuse osteoporosis mouse experimental method, which comprises:

[0085] S1, obtaining the body parameters of the mouse, including the size of the mouse's hip and abdomen, the distance from the mouse's head to the hip and abdomen, and the mouse's body length; and constructing the disuse osteoporosis mouse experimental device according to the body parameters of the mouse;

[0086] Specifically, the size of the mouse's hip and abdomen circumference includes the body width and thickness of the mouse at the hip and abdomen; the body length of the mouse is the distance from the mouse's head to the base of the tail.

[0087] The disuse osteoporosis mouse experimental device is constructed according to the body parameters of the mouse; and the disuse osteoporosis mouse experimental device is installed on the hip and abdomen of the mouse.

[0088] Specifically, the size of the first through hole in the disuse osteoporosis mouse experimental device is determined according to the body width and thickness of the mouse at the hip and abdomen; the height of the first through hole, the thickness of the support member, the height of the support member, etc. are determined according to the distance from the mouse's head to the hip and abdomen and the mouse's body length.

[0089] In a specific embodiment, the width and thickness of the mouse's body at the hip and abdomen are both 1.5 cm, the distance from the head to the hip and abdomen is 6 cm, and the length from the head to the tail root is 6.5 cm; Figure 1A and Figure 1B In the disuse osteoporosis mouse experimental device shown, the diameter of the first through hole is determined to be 1.55 cm, the height of the center point of the first through hole is 3 cm, and the thickness of the support member is 1.2 cm.

[0090] S2, installing the disuse osteoporosis mouse experimental device on the hip and abdomen of the mouse;

[0091] Specifically, the position between the support member and the mouse is adjusted so that the mouse's hip and abdomen are located in the first through hole, so that the mouse's front limbs can crawl and the hind limbs are off the ground and completely located outside the support member; the mouse's tail is adhered to the upper end of the support member using 3M medical breathable tape.

[0092] Furthermore, in order to more conveniently adjust the position between the mouse and the support, the mouse is anesthetized before being placed in the support;

[0093] Specifically, mice were anesthetized by intraperitoneal injection of 1% sodium pentobarbital at a dose of 40 mg / kg.

[0094] S3, detecting physiological indicators of the femur of the mouse.

[0095] In order to demonstrate the effectiveness of the device and the method described in the present invention, an experimental model was constructed using the disuse osteoporosis mouse experimental device and the disuse osteoporosis mouse experimental method described above, and a modeling group and a control group were set up respectively; wherein, the mice in the control group did not receive any treatment, and the mice in the modeling group were modeled using the disuse osteoporosis mouse experimental device for 1 month.

[0096] The experimental results are as follows Figure 6 and Fig.10 As shown; among them, Figure 6 Displayed as using Figure 1A and Figure 1B The shown experimental device for disuse-induced osteoporosis in mice shows the condition of the hind limbs of the mice one month after modeling; Figure 6 Figure A is the control group (Control), and Figure B is the modeling group (Unloading). It can be seen that the hind limb muscles of the mice in the modeling group were significantly atrophied. Figure 7 Shown are the detection results and quantitative statistics of new bone formation in mouse femur; Figure 8 Shows the results and quantitative statistics of bone resorption in rat femur; Fig. 9 Displays the test results and quantitative statistics of rat cancellous bone mass; Fig.10 Shown are the results and quantitative statistics of mouse cortical bone detection; among them, Figures 7 to 10 Each A figure in the figure is the control group (Control), B figure is the modeling group (Unloading), C figure is the quantitative statistical graph, MAR represents the mineral deposition rate, Oc.N / B.Pm represents the number of osteoclasts per unit length of bone tissue, BV / TV represents the volume fraction of cancellous bone tissue, and Ct.Th represents the cortical bone thickness. Figure 7 It is clearly seen that the mineral deposition rate of the modeling group is significantly reduced; Figure 8It is obvious that the number of osteoclasts in the modeling group increased significantly. Fig. 9 It is obvious that the cancellous bone mass in the modeling group is significantly reduced. Fig.10 It was clearly seen that the cortical bone in the modeling group was significantly thinner.

[0097] Therefore, through Figures 6 to 10 It can be seen that constructing an experimental model using the above-mentioned disuse osteoporosis mouse experimental device and the above-mentioned disuse osteoporosis mouse experimental method can achieve better experimental results.

[0098] It should be noted that the disuse osteoporosis mouse experimental device and experimental method provided by the present invention are also applicable to other rodents for constructing disuse osteoporosis animal experiments. In summary, the disuse osteoporosis mouse experimental device and experimental method provided by the present invention can keep the mouse's hind limbs away from the crawling surface, achieve the disuse of the hind limbs, do not affect the normal activities of the mouse's forelimbs, and avoid trauma to the mouse, thereby achieving a good disuse osteoporosis mouse experimental effect. In addition, the experimental device provided by the present invention can be adapted to mice of different sizes by setting an adjustment unit and an extrusion structure, thereby improving the ease of use and expanding the scope of application; in addition, the experimental device provided by the present invention also has the advantages of low cost, small size, and easy expansion. Therefore, the present invention effectively overcomes the various shortcomings in the prior art and has a high industrial utilization value.

[0099] The above embodiments are merely illustrative of the principles and effects of the present invention, and are not intended to limit the present invention. Anyone familiar with the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by a person of ordinary skill in the art without departing from the spirit and technical concept disclosed by the present invention shall still be covered by the claims of the present invention.

Claims

1. A disuse osteoporosis mouse experimental device, characterized in that: The device comprises a support member capable of lifting the mouse's hip and abdomen, a first through hole for supporting the mouse's hip and abdomen to enable the mouse's hind limbs to leave the crawling surface is provided in the middle of the support member; the size of the first through hole is adapted to the size of the mouse's hip and abdomen; the upper part of the support member is used to fix the mouse's tail; the upper part of the support member is provided with a second through hole for passing and fixing the mouse's tail; the bottom of the support member is provided with a roller; The height of the center point of the first through hole on the longitudinal section of the support member is 0.27 to 0.84 times the distance between the mouse hip abdomen and the mouse head; and the thickness of the support member is 1 / 8 to 1 / 6 of the mouse body length; The disuse osteoporosis mouse experimental device also includes at least one group of first extrusion members and a first adjustment unit configured to adjust the size of the first through hole, and at least one group of second extrusion members and a second adjustment unit configured to adjust the size of the second through hole; wherein the first extrusion member is arranged in the first through hole and is connected to the support member by connecting to the first adjustment unit; the second extrusion member is arranged in the second through hole and is connected to the support member by connecting to the second adjustment unit.

2. The disuse osteoporosis mouse experimental device according to claim 1, characterized in that: The single group of the first adjustment unit includes a first threaded rod and a first rotating member; a first threaded through hole adapted to the first threaded rod is provided in the support member, and the first threaded through hole is threadedly connected to the first threaded rod; and one end of the first threaded rod is connected to the first rotating member, and the other end is connected to the first extrusion member.

3. The experimental device for disuse osteoporosis in mice according to claim 2, characterized in that: A first support seat is provided on the outer side of the support member at the connection between the first threaded rod and the first threaded through hole and is sleeved on the outer side of the thread of the first threaded rod.

4. The experimental device for disuse osteoporosis in mice according to claim 1, characterized in that: A single group of the second adjustment unit includes a second threaded rod and a second rotating member; a second threaded through hole adapted to the second threaded rod is provided in the support member, and the second threaded through hole is threadedly connected to the second threaded rod; and one end of the second threaded rod is connected to the second rotating member, and the other end is connected to the second extrusion member.

5. The experimental method of the disuse osteoporosis mouse experimental device according to any one of claims 1 to 4, characterized in that: The experimental method comprises the following steps: Obtaining the body parameters of the mouse, including the size of the mouse's hip and abdomen, the distance from the mouse's head to the hip and abdomen, and the mouse's body length; constructing the disuse osteoporosis mouse experimental device based on the mouse's body parameters; Installing the disuse osteoporosis mouse experimental device on the hip and abdomen of the mouse; The physiological indexes of the femur of the mouse were detected.

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