An observation light supplement device for ultra-high pressure biological culture chamber

Abstract

The present invention discloses an observation and supplementary lighting device for an ultra-high pressure biological culture chamber, comprising a culture chamber shell and a chamber cover, wherein a locking nut is connected below the chamber cover, a structured light-transmitting sheet group is arranged between the locking nut and the chamber cover, and the structured light-transmitting sheet group comprises a quartz glass sheet and an upper acrylic gasket and a lower acrylic gasket arranged on both sides of the quartz glass sheet; a stepped observation hole is provided on the chamber cover, the stepped observation hole is arranged above the structured light-transmitting sheet group, and the chamber cover is provided with a plurality of light-transmitting holes in the circumferential direction of the stepped observation hole. The present invention effectively solves the problem of combining glass with a metal window seat by arranging a quartz glass sheet between two upper and lower acrylic gaskets to form a structured light-transmitting sheet group with a "sandwich" structure, utilizing the characteristics of quartz glass, stainless steel and acrylic materials.

Description

Technical Field

[0001] The invention relates to the technical field of biological culture chambers, and in particular to an observation light supplement device used in an ultra-high pressure biological culture chamber. Background Art

[0002] In 1899, American chemists first discovered that ultra-high pressure of 450MPa can prolong the shelf life of milk. Later, there were reports of ultra-high pressure sterilization. However, due to the conditions at that time, these research results did not attract enough attention and were not promoted and applied in actual production. In the late 1980s, many countries invested a lot of manpower, material and financial resources, and people resumed the research and application of ultra-high pressure technology in the fields of biology and food.

[0003] Ultra-high pressure food meets the consumer demand for simple, safe, natural and nutritious new foods in the 21st century, and sometimes produces strange effects. For example, when 100MPa ultra-high pressure is applied to old rice, it tastes like new rice. my country's research and industrial application of ultra-high pressure technology is catching up with Western developed countries such as the United States and Japan, and has also achieved gratifying results.

[0004] The so-called ultra-high pressure processing technology uses ultra-high pressure greater than 100MPa to perform isostatic pressure treatment on organisms and foods. The non-common bonds such as hydrogen bonding, hydrophobic bonding, and ionic bonding in the molecular three-dimensional structure change, causing physical, chemical and biological effects such as enzyme inactivation, protein denaturation, starch gelatinization and microbial inactivation, thereby achieving the effect of sterilization and modification, while the flavor substances, vitamins, pigments, etc. of the food are not changed, and the loss of nutrients is very small. It is particularly suitable for processing heat-sensitive foods, foods with high active ingredients such as vitamins, probiotics, unsaturated fatty acids, carotenoids, etc. and fresh foods with high added value.

[0005] The deep sea is a world of high pressure, darkness and coldness. The pressure at the bottom of the Mariana Trench is as high as 110MPa. Exploring the deep sea is a huge challenge for humans. Surprisingly, scientists have seen marine life swimming at such a deep seabed.

[0006] The production process of the Jiaolong observation window: The glass and metal window seat of the observation window are inlaid as a whole and are in direct contact with seawater. If the precision of the two is not enough, leakage will occur at the window glass. The gap between the glass and the metal window seat must be controlled below 0.2 silk. 0.2 silk is about one-fiftieth of a hair. Such a small installation gap cannot be measured by any metal instrument. Once the glass is scratched with a small scratch, it may become the starting point for the glass to burst under the heavy pressure of the deep sea. This work can only be judged by a craftsman of a great country with his eyes and hands, and pure manual polishing and maintenance to determine the error of 0.2 silk. The gap between the glass and the metal window seat is a major obstacle to deep-sea exploration and simulation of deep-sea environment, so there is an urgent need for an observation and supplementary lighting device for ultra-high pressure biological culture chambers. Summary of the invention

[0007] The purpose of the present invention is to provide an observation light supplement device for an ultra-high pressure biological culture chamber to solve the problems existing in the above-mentioned prior art.

[0008] To achieve the above-mentioned purpose, the present invention provides the following solution: The present invention provides an observation and supplementary lighting device for an ultra-high pressure biological culture chamber, comprising a culture chamber shell and a chamber cover, wherein the culture chamber shell and the chamber cover are detachably connected; a locking nut is connected below the chamber cover, and a structured light-transmitting sheet group is arranged between the locking nut and the chamber cover, wherein the structured light-transmitting sheet group comprises a quartz glass sheet and an upper acrylic gasket and a lower acrylic gasket arranged on both sides of the quartz glass sheet.

[0009] The hatch cover is provided with a stepped observation hole, which is arranged above the structured light-transmitting sheet group. The hatch cover is provided with a plurality of light-transmitting holes in the circumferential direction of the stepped observation hole.

[0010] Preferably, the hatch cover is a disc structure, and the hatch cover and the culture chamber shell are connected by bolts and a clamping ring; a cylinder is arranged below the hatch cover, the cylinder is adapted to the inner cavity of the culture chamber shell, and a seal is arranged between the cylinder and the culture chamber shell.

[0011] Preferably, a clamp is connected between the hatch cover and the culture chamber shell, a clamp protrusion matched with the clamp is provided on the hatch cover, and a clamp groove matched with the clamp is provided on the culture chamber shell.

[0012] Preferably, the locking nut is a concave locking nut, and the lower end surface of the hatch cover is provided with a threaded protrusion that is compatible with the locking nut.

[0013] Preferably, a sealing ring is provided on the inner side of the locking nut, and a groove for accommodating the sealing ring is provided in the locking nut.

[0014] Preferably, the number of the light-transmitting holes is no less than 4, and light-emitting diodes are arranged in the light-transmitting holes, and the colors of the light-emitting diodes include white light, red light, yellow light and blue light.

[0015] The present invention discloses the following technical effects: the present invention forms a structural light-transmitting sheet group with a "sandwich" structure by arranging a quartz glass sheet between two upper and lower acrylic gaskets; the hardness of the acrylic gasket is much smaller than that of quartz glass and stainless steel, and is very easy to grind, and is very easy to bond with quartz glass and stainless steel, thereby ensuring the precision requirement of the structural light-transmitting sheet; at the same time, the Young's modulus of acrylic is 3GPa, and it is easier to deform, and it plays the role of a "rubber pad" between quartz glass and stainless steel, plays a protective role, and effectively solves the problem of combining glass with a metal window seat; at the same time, the light transmittance of acrylic and quartz glass is very close to the refractive index in liquid, and the imaging effect is good. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0017] Figure 1 is a schematic diagram of the structure of the culture capsule shell of the present invention,

[0018] Figure 2 yes Figure 1 A partial enlarged view of the middle A.

[0019] Figure 3 It is a schematic diagram of the structure of the light-transmitting hole and the stepped observation hole.

[0020] Among them, 1. hatch cover, 2. clamp, 3. culture chamber shell, 11. stepped observation hole, 12. light-transmitting hole, 13. upper acrylic gasket, 14. quartz glass sheet, 15. acrylic gasket, 16. sealing ring, 17. locking nut, 18. cylinder, 19. clamp protrusion, 20. clamp groove, 21. threaded protrusion. DETAILED DESCRIPTION

[0021] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0023] Reference Figure 1-3 The present invention provides an observation and supplementary lighting device for an ultra-high pressure biological culture chamber, comprising a culture chamber shell 3 and a chamber cover 1. The chamber cover 1 is a disc structure, and the chamber cover 1 and the culture chamber shell 3 are connected by bolts and clamps. A cylinder 18 is arranged below the chamber cover 1, and the cylinder 18 is adapted to the inner cavity of the culture chamber shell 3. A sealing member is arranged between the cylinder 18 and the culture chamber shell 3, so as to ensure that the culture chamber shell 3 and the chamber cover 1 are tightly connected and improve the sealing performance. A clamp 2 is connected between the chamber cover 1 and the culture chamber shell 3, and a clamp protrusion 19 adapted to the clamp 2 is arranged on the chamber cover 1, and a clamp groove 20 adapted to the clamp 2 is arranged on the culture chamber shell 3. The culture chamber shell 3 and the chamber cover 1 are detachably connected by threads and the clamp 2, so as to ensure the understanding strength between the two. The chamber cover 1, the clamp 2, and the culture chamber body 3 are all made of stainless steel.

[0024] A locking nut 17 is connected to the bottom of the hatch cover 1. The locking nut 17 is a concave locking nut. An internal thread is provided inside the locking nut 17. A threaded protrusion 21 matching the locking nut 17 is provided on the lower end surface of the hatch cover 1. A sealing ring 16 is provided on the inner side of the locking nut 17. A groove for accommodating the sealing ring 16 is provided inside the locking nut 17.

[0025] A structured light-transmitting sheet group is arranged between the locking nut 17 and the hatch 1, and the structured light-transmitting sheet group is fixedly connected to the hatch 1 by the locking nut 17. The structured light-transmitting sheet group includes a quartz glass sheet 14 and an upper acrylic gasket 13 and a lower acrylic gasket 15 arranged on both sides of the quartz glass sheet 14; the structured light-transmitting sheet group is a "sandwich" structure, that is, two acrylic gaskets sandwich a piece of quartz glass, and their diameters are the same and smaller than the diameter of the threaded protrusion 21. Quartz glass is a brittle material, and stainless steel is a plastic material. When subjected to ultra-high pressure external force, stainless steel can undergo elastic deformation, while quartz glass can only undergo slight deformation. If the quartz glass is deformed greatly, it will break. In addition, the Young's modulus of quartz glass is 72GPa, and the Young's modulus of stainless steel is 195GPa. When subjected to the same external force, the quartz glass will deform first. The present invention adopts a "sandwich" structure to solve the problem of combining glass and metal window seats. Acrylic is "softer" than quartz glass and stainless steel, and is very easy to grind, which means it is very easy to bond with quartz glass and stainless steel. In addition, the Young's modulus of acrylic is 3GPa, which is easier to deform, and it plays the role of a "rubber pad" between quartz glass and stainless steel. At the same time, the light transmittance of acrylic and quartz glass is very close to the refractive index in liquid, so the imaging effect is good.

[0026] A stepped observation hole 11 is provided along the axis of the hatch cover 1, and the aperture is small inside and large outside, which ensures the pressure bearing capacity; the stepped observation hole 11 is arranged above the structural light-transmitting sheet group, and the hatch cover 1 is provided with a plurality of light-transmitting holes 12 in the circumferential direction of the stepped observation hole 11 to reduce the concentrated force area. There are no less than 4 light-transmitting holes 12, and light-emitting diodes are arranged in the light-transmitting holes 12. The colors of the light-emitting diodes include white light, red light, yellow light and blue light. These three colors of light can be used alone or in pairs to form more colors of light, so as to study the tolerance of organisms under different light colors. An endoscope camera lens is arranged in the stepped observation hole 11, and the biological changes in the culture cabin can be observed through the screen at any time, which is convenient for observation and video recording, and the survival status of the organisms can be observed at any time.

[0027] In the description of the present invention, it should be understood that the terms "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside" and "outside" etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0028] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. An observation light supplement device for an ultra-high pressure biological culture chamber, characterized in that: The invention comprises a culture chamber shell (3) and a chamber cover (1), wherein the culture chamber shell (3) and the chamber cover (1) are detachably connected; a locking nut (17) is connected below the chamber cover (1); a structured light-transmitting sheet group is arranged between the locking nut (17) and the chamber cover (1); the structured light-transmitting sheet group is connected to the chamber cover (1) via the locking nut (17); the structured light-transmitting sheet group comprises a quartz glass sheet (14) and an upper acrylic gasket (13) and a lower acrylic gasket (15) arranged on both sides of the quartz glass sheet (14); the structured light-transmitting sheet group is a "sandwich" structure, i.e., a piece of the quartz glass sheet (14) is sandwiched between the upper acrylic gasket (13) and the lower acrylic gasket (15); The hatch cover (1) is provided with a stepped observation hole (11), the stepped observation hole (11) is arranged above the structured light-transmitting sheet group, and the hatch cover (1) is provided with a plurality of light-transmitting holes (12) in the circumferential direction of the stepped observation hole (11); The lower end surface of the hatch cover (1) is provided with a threaded protrusion (21) adapted to fit the locking nut (17).

2. The observation light supplement device for an ultrahigh pressure biological culture chamber according to claim 1, characterized in that: The hatch cover (1) is a disc-type structure, and the hatch cover (1) and the culture chamber shell (3) are connected by bolts and a clamping ring; a cylinder (18) is arranged below the hatch cover (1), the cylinder (18) is adapted to the inner cavity of the culture chamber shell (3), and a sealing member is arranged between the cylinder (18) and the culture chamber shell (3).

3. The observation light supplement device for an ultrahigh pressure biological culture chamber according to claim 1, characterized in that: A clamp (2) is connected between the hatch cover (1) and the culture chamber shell (3); a clamp protrusion (19) matching the clamp (2) is provided on the hatch cover (1); and a clamp groove (20) matching the clamp (2) is provided on the culture chamber shell (3).

4. The observation light supplement device for an ultrahigh pressure biological culture chamber according to claim 1, characterized in that: The locking nut (17) is a concave locking nut.

5. The observation light supplement device for an ultrahigh pressure biological culture chamber according to claim 4, characterized in that: A sealing ring (16) is arranged on the inner side of the locking nut (17), and a groove for accommodating the sealing ring (16) is provided in the locking nut (17).

6. The observation light supplement device for an ultrahigh pressure biological culture chamber according to claim 1, characterized in that: The light-transmitting holes (12) are provided with no less than four, and light-emitting diodes are arranged in the light-transmitting holes (12), and the colors of the light-emitting diodes include white light, red light, yellow light and blue light.

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