An initiator for aged corn seeds and its treatment method

Abstract

The application relates to an aging corn seed priming agent and a treatment method. The priming agent comprises phloretin and hydroxypropyl-beta-cyclodextrin, and the concentration of the phloretin is 50-200 mg / L. The aging corn seed priming agent provided by the application can significantly improve the germination and seedling growth of aging corn.

Description

Technical Field

[0001] This invention relates to an initiator for aged corn seeds and a treatment method thereof. Background Technology

[0002] my country is the world's second-largest corn producer, accounting for 17.2% of the world's total corn acreage. With population growth and decreasing arable land, corn, as a crucial food and feed crop, holds an extremely important position in my country's national economy and has become a significant factor influencing the balance of food supply and demand. In addition, corn is indispensable in animal husbandry, requiring large quantities of corn as feed additives. Corn is 70% starch, and the demand for corn in the industrial raw material market is constantly increasing. Due to the large size and high fat content of corn embryos, corn seeds have poor storage stability and are prone to aging. Therefore, after a certain period of storage, corn seeds inevitably undergo natural aging, resulting in damage to the cell membrane system, reduced enzyme activity, and other physiological functions, leading to a decline in field germination rate and seedling quality, causing economic losses to corn production. Therefore, how to promote the germination of aging corn seeds and ensure corn production is a technical problem that needs to be solved. Summary of the Invention

[0003] The purpose of this invention is to overcome the shortcomings and deficiencies of the existing technology and to provide an initiator and treatment method for aged corn seeds.

[0004] The technical solution adopted in this invention is as follows: an initiator for aged corn seeds, comprising phloretin and hydroxypropyl-β-cyclodextrin, wherein the concentration of phloretin is 50-200 mg / L.

[0005] Preferably, the mass ratio of phloretin to hydroxypropyl-β-cyclodextrin is 1:4-8.

[0006] Furthermore, the mass ratio of phloretin to hydroxypropyl-β-cyclodextrin is 1:6.

[0007] Preferably, it also includes betaine.

[0008] Preferably, the mass ratio of phloretin to betaine is 1:0-4.

[0009] Furthermore, the mass ratio of phloretin to betaine is 1:3.

[0010] A method for treating aged corn seeds includes the following steps: soaking the aged corn seeds in an aged corn seed initiator as described above.

[0011] The beneficial effects of the present invention are as follows: The aging corn seed initiator provided by the present invention can significantly improve the germination and seedling growth of aging corn. Detailed Implementation

[0012] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be described in further detail below.

[0013] The corn seeds used in the following examples and comparative examples are Jinyutian No. 2 variety. Artificial accelerated aging treatment was performed on the corn seeds, and the specific operation was as follows: A 2-3 cm layer of water was added to the bottom of the desiccant. A layer of clean gauze was laid on a white porcelain plate, and then the corn seeds were evenly placed on the gauze. The desiccant was closed and placed in a sealed constant temperature chamber. After treatment for 5 days at a temperature of 43±2℃ and a relative humidity of 85%, the corn seeds were removed and dried in a drying oven at 33±2℃ until their original moisture content was reached. Before the artificial aging treatment, the germination rate of the corn seeds was 95%.

[0014] Hydroxypropyl-β-cyclodextrin, phlorizin, and betaine were added to water and stirred for 30 minutes to obtain a soaking system. Corn seeds were then placed in the soaking system, placed on a shaker, and soaked at room temperature for 6 hours. After soaking, the seeds were removed, rinsed with clean water, and air-dried. The control group (CK) was soaked in clean water for 6 hours.

[0015] Plant corn seeds in flowerpots, sowing evenly with 100 seeds per group, repeating 3 times, and counting the germination rate. Five weeks after planting, randomly select 3 corn plants from each group and measure the seedling's bud length, root length, fresh weight, and dry weight, repeating 3 times and calculating the average value.

[0016] Example 1:

[0017] Phloretin was set at 50 mg / L, and hydroxypropyl-β-cyclodextrin was set at concentrations of 0, 50, 100, 200, 300, 400, 500, 600, and 800 mg / L, with betaine set at 0.

[0018] As shown in Table 1, when only 50 mg / L of phloretin was added (experimental group 1), the germination rate was slightly improved, but the improvement effect was average. When a certain amount of hydroxypropyl-β-cyclodextrin was added, the germination rate was significantly improved, and various growth indicators of seedlings were improved. When the mass ratio of hydroxypropyl-β-cyclodextrin to phloretin was 4-8:1, the effect was better. When the mass ratio of hydroxypropyl-β-cyclodextrin to phloretin was 6:1, the effect was the best. At this time, the germination rate was increased by 84% compared with the control group, the shoot length was increased by 67%, the root length was increased by 76%, the fresh weight was increased by 67%, and the dry weight was increased by 86%.

[0019]

[0020] Example 2:

[0021] Phloretin was set at 100 mg / L, and hydroxypropyl-β-cyclodextrin was set at 0, 200 mg / L, 400 mg / L, 800 mg / L, 1200 mg / L, and 1600 mg / L, respectively. Betaine was set at 0.

[0022] As shown in Table 2, when only 100 mg / L phlorizin was added (experimental group 1), the germination rate was slightly improved compared to the control group and when only 50 mg / L phlorizin was added. When a certain amount of hydroxypropyl-β-cyclodextrin was added, the germination rate was significantly improved, and various growth indicators of the seedlings were also improved. The best effect was achieved when the mass ratio of hydroxypropyl-β-cyclodextrin to phlorizin was 6:1, at which time the germination rate was increased by 96%, the shoot length by 65%, the root length by 85%, the fresh weight by 73%, and the dry weight by 110% compared to the control group.

[0023]

[0024] Example 3:

[0025] With phlorizin at 150 mg / L, hydroxypropyl-β-cyclodextrin at 900 mg / L, and betaine at 0%, the germination rate was 58.67%, an increase of 85% compared to the control group; the shoot length was 9.21 cm, an increase of 62% compared to the control group; the root length was 5.84 cm, an increase of 84% compared to the control group; the fresh weight was 0.3559 g, an increase of 73% compared to the control group; and the dry weight was 0.2308 g, an increase of 99% compared to the control group.

[0026] Example 4:

[0027] With phlorizin at 200 mg / L, hydroxypropyl-β-cyclodextrin at 1200 mg / L, and betaine at 0%, the germination rate was 44.33%, a 40% increase compared to the control group; the shoot length was 8.41 cm, a 48% increase compared to the control group; the root length was 3.96 cm, a 25% increase compared to the control group; the fresh weight was 0.2968 g, a 44% increase compared to the control group; and the dry weight was 0.1760 g, a 52% increase compared to the control group.

[0028] Example 5:

[0029] The concentrations of phloretin, hydroxypropyl-β-cyclodextrin, and betaine were set at 50 mg / L, 300 mg / L, and 0, 50, 100, 150, 200, and 250 mg / L, respectively.

[0030] As shown in Table 3, the addition of an appropriate amount of betaine significantly improved the germination rate and various growth indicators of the seedlings. The best results were achieved when the mass ratio of betaine to root bark extract was 3:1, at which point the germination rate increased by 108%, the shoot length increased by 72%, the root length increased by 88%, the fresh weight increased by 85%, and the dry weight increased by 120% compared to the control group.

[0031]

[0032] Example 6:

[0033] With phlorizin at 100 mg / L, hydroxypropyl-β-cyclodextrin at 600 mg / L, and betaine at 300 mg / L, the germination rate was 64.33%, an increase of 103% compared to the control group; the shoot length was 9.61 cm, an increase of 69% compared to the control group; the root length was 6.22 cm, an increase of 96% compared to the control group; the fresh weight was 0.3926 g, an increase of 91% compared to the control group; and the dry weight was 0.2538 g, an increase of 119% compared to the control group.

[0034] Comparative Example 1:

[0035] With phloretin at 250 mg / L and hydroxypropyl-β-cyclodextrin at 1500 mg / L, the germination rate was 28.67%, a decrease of 9% compared to the control group; the shoot length was 5.48 cm, a decrease of 4% compared to the control group; the root length was 2.25 cm, a decrease of 29% compared to the control group; the fresh weight was 0.1905 g, a decrease of 7% compared to the control group; and the dry weight was 0.0869 g, a decrease of 25% compared to the control group.

[0036] Comparative Example 2:

[0037] With phloretin at a concentration of 250 mg / L and hydroxypropyl-β-cyclodextrin at a concentration of 500 mg / L, the germination rate was 27.33%, a decrease of 14% compared to the control group; the shoot length was 5.59 cm, a decrease of 2% compared to the control group; the root length was 2.31 cm, a decrease of 27% compared to the control group; the fresh weight was 0.1969 g, a decrease of 4% compared to the control group; and the dry weight was 0.0828 g, a decrease of 29% compared to the control group.

[0038] Comparative Example 3:

[0039] With phloretin at 0 and hydroxypropyl-β-cyclodextrin at 500 mg / L, the germination rate was 31.33%, the shoot length was 5.74 cm, the root length was 3.16 cm, the fresh weight was 0.2084 g, and the dry weight was 0.1149 g. Compared with the control group, there were no significant changes.

[0040] Comparative Example 4:

[0041] With phlorizin and hydroxypropyl-β-cyclodextrin levels set at 0, and betaine concentration at 150 mg / L, the germination rate was 26.33%, the shoot length was 5.51 cm, the root length was 3.11 cm, the fresh weight was 0.2105 g, and the dry weight was 0.0968 g. 150 mg / L of betaine had a certain inhibitory effect on corn seed germination.

[0042] The above description discloses only preferred embodiments of the present invention and should not be construed as limiting the scope of the present invention. Therefore, equivalent variations made in accordance with the claims of the present invention are still within the scope of the present invention.

Claims

1. An aging corn seed priming agent, characterized by: phloretin and hydroxypropyl-β-cyclodextrin, wherein the concentration of phloretin is 50-200 mg / L; the mass ratio of the phloretin and the hydroxypropyl-β-cyclodextrin is 1:6; further comprising betaine; the mass ratio of the phloretin and the betaine is 1:

3.

2. A method of treating aging corn seed, comprising comprising the following steps: immersing the aging corn seeds in the aging corn seed priming agent as claimed in claim 1.

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