A single-cell nucleic acid processing instrument

Abstract

The present invention relates to the technical field of single-cell nucleic acid processing, and discloses a single-cell nucleic acid processing instrument, comprising: a frame; an air pump assembly, arranged on the frame; a box body structure, comprising a box body drive assembly and a box body assembly, the box body drive assembly can drive the box body assembly to move in the horizontal direction, and the box body assembly is provided with a placement slot; a box cover structure, comprising a box cover drive assembly and a box cover assembly, the box cover drive assembly can drive the box cover assembly to move in the vertical direction so that the box cover assembly is covered on the box body assembly, the box cover assembly includes a box cover body, and the box cover body is provided with an air inlet hole group and an air extraction hole group, and the air inlet hole group and the air extraction hole group are respectively connected to the air pump assembly; a chip structure, arranged in the placement slot. The single-cell nucleic acid processing instrument disclosed by the present invention has a simple structure, a higher degree of automation, a shorter experiment time, simpler experimental operation, and higher customer satisfaction.

Description

Technical Field

[0001] The present invention relates to the technical field of single-cell nucleic acid processing, and in particular to a single-cell nucleic acid processing instrument. Background Art

[0002] Single-cell nucleic acid processing instruments are used to extract DNA or RNA from cells. Existing single-cell nucleic acid processing instruments have the disadvantages of complex structure and low degree of automation, resulting in cumbersome operation and long experimental time in actual operation, which reduces customer satisfaction. Summary of the Invention

[0003] Based on the above, the object of the present invention is to provide a single-cell nucleic acid processing instrument with the advantages of simple structure and high degree of automation.

[0004] To achieve the above object, the present invention adopts the following technical solutions:

[0005] A single-cell nucleic acid processing instrument comprises: a frame; an air pump assembly, which is arranged on the frame; a box structure, which comprises a box drive assembly and a box assembly, wherein the box drive assembly is arranged on the frame, the box assembly is arranged at the output end of the box drive assembly, the box drive assembly can drive the box assembly to move in a horizontal direction, and the box assembly is provided with a placement slot; a box cover structure, which comprises a box cover drive assembly and a box cover assembly, wherein the box cover drive assembly is arranged on the frame, the box cover assembly is arranged at the output end of the box cover drive assembly and is positioned Above the box body assembly, the box cover driving assembly can drive the box cover assembly to move in the vertical direction so that the box cover assembly is covered on the box body assembly, the box cover assembly includes a box cover body, and the box cover body is provided with an air inlet hole group and an air extraction hole group, and the air inlet hole group and the air extraction hole group are both connected to the air pump assembly; a chip structure is arranged in the placement groove, and the chip structure is provided with a sample loading groove, a sample groove, a waste liquid groove and a microchannel, the sample loading groove is connected to the air inlet hole group, and the sample groove and the waste liquid groove are both connected to the air extraction hole group.

[0006] As a preferred solution of a single-cell nucleic acid processing instrument, the box cover assembly includes a first air intake control valve, the air intake hole group includes at least two air intake holes, each of the air intake holes is provided with a first air intake control valve, the first air intake control valve is configured to control the connection or disconnection between the air intake hole and the air pump assembly, the box cover body is also provided with an air intake channel, one end of the air intake channel is respectively connected to at least two of the air intake holes, and the other end is connected to the air pump assembly; the box cover assembly includes a first air extraction control valve and a second air extraction control valve, the air extraction The hole group includes two air extraction holes, which are respectively a first air extraction hole and a second air extraction hole. The first air extraction hole is connected to the waste liquid tank and is provided with the first air extraction control valve, and the second air extraction hole is connected to the sample tank and is provided with the second air extraction control valve. The first air extraction control valve and the second air extraction control valve are configured to control the connection or disconnection between the air extraction holes and the air pump assembly. An air extraction channel is also provided on the box cover body, one end of the air extraction channel is connected to the two air extraction holes, and the other end of the air extraction channel is connected to the air pump assembly.

[0007] As a preferred embodiment of a single-cell nucleic acid processing instrument, the single-cell nucleic acid processing instrument also includes an air path integrated block, the air path integrated block is provided with a first connecting channel, a second connecting channel and a first detection channel, one end of the first connecting channel is connected to the air pump assembly, the other end of the first connecting channel is connected to the same end of the second connecting channel and the first detection channel, the other end of the second connecting channel is connected to the air intake channel, and the other end of the first detection channel can be connected to the pressure detector; the air path integrated block is also provided with a third connecting channel, a fourth connecting channel and a second detection channel, one end of the third connecting channel is connected to the exhaust channel, the other end of the third connecting channel is connected to the same end of the fourth connecting channel and the second detection channel, the other end of the fourth connecting channel is connected to the air pump assembly, and the other end of the second detection channel can be connected to the pressure detector.

[0008] As a preferred solution of a single-cell nucleic acid processing instrument, the other end of the first detection channel is interference-fitted with a first plug, and the other end of the second detection channel is interference-fitted with a second plug.

[0009] As a preferred solution of a single-cell nucleic acid processing instrument, the air pump assembly includes: an air pump motor, which is arranged on the frame; an air pump ball screw, including an air pump screw and an air pump nut, the air pump screw is arranged at the output end of the air pump motor, and the air pump nut is threadedly connected to the air pump screw; an air pump body, including an air pump barrel and an air pump push rod assembly, the air pump barrel is arranged on the frame and defines an air pump cavity therein, the air pump cavity can be respectively connected to the air inlet hole group and the air extraction hole group, one end of the air pump push rod assembly is sealingly and slidingly connected to the air pump barrel, and the other end of the air pump push rod assembly is fixedly connected to the air pump nut.

[0010] As a preferred solution for a single-cell nucleic acid processing instrument, the box lid drive assembly includes two box lid motor assemblies, and the two box lid motor assemblies are respectively located on the left and right sides of the box lid body, and each of the box lid motor assemblies includes: a box lid motor body, which is arranged on the frame; a box lid ball screw, including a box lid screw and a box lid nut, the box lid screw is arranged at the output end of the box lid motor body, the box lid nut is threadedly connected to the box lid screw, and the box lid nut is fixedly connected to the box lid body.

[0011] As a preferred solution for a single-cell nucleic acid processing instrument, the frame includes a placement plate, the box cover motor body is arranged on the lower side of the placement plate, the box cover drive assembly also includes two box cover conveying assemblies, the box cover conveying assemblies are arranged on the upper side of the placement plate, and each of the box cover conveying assemblies includes: a first box cover gear, fixedly arranged at the output end of the box cover motor body; a second box cover gear, fixedly connected to the box cover screw rod; and a box cover conveyor belt, meshing with the first box cover gear and the second box cover gear.

[0012] As a preferred solution for a single-cell nucleic acid processing instrument, the box body drive assembly includes: a first box body motor, which is arranged on the frame; a box body ball screw, including a box body screw and a box body nut, the box body screw is arranged at the output end of the first box body motor, the box body nut is threadedly connected to the box body screw and fixedly connected to the box body assembly, and the first box body motor can drive the box body screw to rotate so that the box body nut drives the box body assembly to move along the horizontal direction.

[0013] As a preferred solution for a single-cell nucleic acid processing instrument, the box body drive assembly includes: a second box body motor, which is arranged on the frame; a box body gear transmission assembly, including a meshing box body gear and a box body rack, the box body gear is fixedly arranged at the output end of the second box body motor, the box body rack is slidably arranged on the frame and fixedly connected to the box body assembly, and the second box body motor can drive the box body gear to rotate so that the box body rack drives the box body assembly to move along the horizontal direction.

[0014] As a preferred solution for a single-cell nucleic acid processing instrument, the box body assembly includes a box body, a heat preservation frame and a heat-conducting assembly. The heat-conducting assembly and the box body form a chamber with an open upper end. The heat-conducting assembly includes a heat-conducting part, a Peltier and a heat sink stacked in sequence. The Peltier is located below the heat-conducting part and can heat and cool the chamber. The heat sink is located below the Peltier. The heat preservation frame is arranged along the circumference of the heat-conducting part and is clamped between the heat-conducting part and the heat sink. The heat preservation frame is provided with an avoidance hole, and the avoidance hole is arranged opposite to the heat-conducting part and the heat sink.

[0015] As a preferred solution for a single-cell nucleic acid processing instrument, the heat sink is a heat sink fin, which is arranged on the box body assembly; or: the heat sink is a heat sink, and a cooling channel is provided in the heat sink, and the cooling water in the cooling channel can cool the heat sink.

[0016] As a preferred embodiment of a single-cell nucleic acid processing instrument, the single-cell nucleic acid processing instrument also includes a magnet feeding structure, the magnet feeding structure includes a magnet driving assembly, a connecting assembly and a magnet arm, the magnet driving assembly is arranged on the frame, the connecting assembly is arranged at the output end of the magnet driving assembly, the connecting assembly includes a first connecting block, a second connecting block and a feeding elastic member, the second connecting block is slidably arranged on the first connecting block, the feeding elastic member is clamped between the first connecting block and the second connecting block, the first connecting block can adsorb the second connecting block when energized, and the feeding elastic member can reset the second connecting block when the power is off, the magnet arm is fixedly arranged on the second connecting block, the magnet driving assembly can drive the connecting assembly to drive the magnet arm to move in the horizontal direction so that the magnet arm is facing the chip structure, and when the first connecting block is energized, the magnet arm can move in the vertical direction toward the direction close to the chip structure with the second connecting block.

[0017] As a preferred solution of a single-cell nucleic acid processing instrument, the chip structure includes a chip box and at least one chip component, at least one chip component is arranged in the chip box, and the chip box is clamped in the placement groove.

[0018] The beneficial effects of the present invention are as follows: the single-cell nucleic acid processing instrument disclosed in the present invention has a simple structure, the box body driving assembly can drive the box body assembly to move in the horizontal direction so that the box body assembly is located directly below the box cover body or the box body assembly is pushed outward, and the box cover driving assembly can drive the box cover assembly to move in the vertical direction so that the box cover body is covered on the box body assembly, the degree of automation is higher, the experiment time is shorter, the experimental operation is simpler, and the customer satisfaction is higher. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the following briefly introduces the drawings required for use in describing the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the contents of the embodiments of the present invention and these drawings without any creative work.

[0020] Figure 1 is a schematic diagram of a single-cell nucleic acid processing instrument provided by a specific embodiment of the present invention in one direction;

[0021] Figure 2 is a schematic diagram of a single-cell nucleic acid processing apparatus provided by a specific embodiment of the present invention in another direction;

[0022] Figure 3 It is a schematic diagram of a chip structure, a box assembly, and a portion of a rack provided by a specific embodiment of the present invention;

[0023] Figure 4 is a cross-sectional view of a chip box and a box body assembly provided by a specific embodiment of the present invention;

[0024] Figure 5 is a cross-sectional view of a box assembly provided by a specific embodiment of the present invention;

[0025] Figure 6 is a schematic diagram of a box assembly provided by a specific embodiment of the present invention;

[0026] Figure 7 It is a schematic diagram of the box cover body provided by a specific embodiment of the present invention in one direction;

[0027] Figure 8 is a schematic diagram of the box cover body provided by a specific embodiment of the present invention in another direction;

[0028] Figure 9 is a cross-sectional view of the box cover body along the AA direction provided by a specific embodiment of the present invention;

[0029] Figure 10 is a cross-sectional view of a box cover assembly provided by a specific embodiment of the present invention;

[0030] Figure 11 2 is a cross-sectional view of the box cover body along the BB direction provided by a specific embodiment of the present invention;

[0031] Figure 12 is a cross-sectional view of the box cover body along the CC direction provided by a specific embodiment of the present invention;

[0032] Figure 13Schematic diagram of a gas path integrated block, a connecting pipe, an adjusting block, an adjusting member, and a connecting joint provided in a specific embodiment of the present invention in a first direction;

[0033] Figure 14 Schematic diagram of the gas path integrated block, connecting pipe, regulating block and regulating member provided in a specific embodiment of the present invention in the second direction;

[0034] Figure 15 It is a schematic diagram of the gas path integrated block, connecting pipe, regulating block and regulating member provided in a specific embodiment of the present invention in a third direction;

[0035] Figure 16 is a cross-sectional view of a magnet feeding structure provided by a specific embodiment of the present invention;

[0036] Figure 17 It is a schematic diagram of a magnet feeding structure provided by a specific embodiment of the present invention.

[0037] In the picture:

[0038] 1. Frame; 11. Mounting plate; 12. Placement plate;

[0039] 21. Air pump body; 211. Air pump barrel; 212. Air pump push rod assembly; 22. Air pump transmission assembly; 221. First air pump gear; 222. Second air pump gear; 223. Air pump transmission belt;

[0040] 30. Placement slot; 31. Box drive assembly; 311. First box motor; 312. Box ball screw; 32. Box assembly; 320. Second slot; 321. Box body; 322. Insulation frame; 3220. Avoidance hole; 323. Heat-conducting assembly; 3231. Heat-conducting element; 3232. Heat sink; 324. Snap-fit ​​guide post; 325. Snap-fit ​​elastic element; 326. Block;

[0041] 41. Lid drive assembly; 411. Lid motor body; 412. Lid conveyor assembly; 4121. First lid gear; 4122. Second lid gear; 4123. Lid conveyor belt; 42. Lid assembly; 421. Lid body; 42101. Air inlet channel; 42102. Air inlet hole; 42103. Air extraction channel; 421041. First air extraction hole; 4210411. First air extraction connecting hole; 4210412. First connecting blind hole; 421042. Second air extraction hole; 421 0421, second air extraction connecting hole; 4210422, second connecting blind hole; 42105, air intake blind hole; 42106, first detection hole; 42107, second detection hole; 42108, air extraction blind hole; 42109, third detection hole; 421010, detection blind hole; 421011, fourth detection hole; 422, first air intake control valve; 423, first air extraction control valve; 424, second air extraction control valve; 425, third air extraction control valve; 426, detection control valve; 427, circuit board;

[0042] 5. Chip structure; 501. Sample loading slot; 502. Sample slot; 503. Waste liquid slot; 51. Chip box; 510. First card slot; 511. Card protrusion; 52. Chip assembly;

[0043] 61. Gas circuit integrated block; 6101. First connecting channel; 6102. Second connecting channel; 6103. First detection channel; 6104. Third connecting channel; 6105. Fourth connecting channel; 6106. Second detection channel; 62. Connector; 63. Connecting pipe; 64. Adjustment block; 65. Adjustment member;

[0044] 71. Magnet drive assembly; 711. Feed motor; 712. Feed ball screw; 72. Connecting assembly; 721. First connecting block; 722. Second connecting block; 723. Feed elastic member; 724. Feed guide column; 73. Magnet arm; 730. Avoidance groove. DETAILED DESCRIPTION

[0045] To make the technical problems solved, the technical solutions adopted, and the technical effects achieved by the present invention more clearly understood, the technical solutions of the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings. It is apparent that the described embodiments are only some of the embodiments of the present invention, not all of them. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort shall fall within the scope of protection of the present invention.

[0046] In the description of the present invention, it should be noted that the terms "center," "up," "down," "left," "right," "vertical," "horizontal," "inside," and "outside" and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate the description of the present invention and simplify the description. They are not intended to indicate or imply that the devices or components referred to must have a specific orientation, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance. The terms "first position" and "second position" refer to two different positions.

[0047] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood broadly. For example, they may refer to fixed or detachable connections; mechanical or electrical connections; direct or indirect connections through an intermediary; and internal communication between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention.

[0048] like Figures 1 to 17 As shown, this embodiment provides a single-cell nucleic acid processing instrument, including a frame 11, an air pump assembly, a box structure, a box cover structure and a chip structure 5, the air pump assembly is arranged on the frame 11, the box structure includes a box drive assembly 31 and a box assembly 32, the box drive assembly 31 is arranged on the frame 11, the box assembly 32 is arranged at the output end of the box drive assembly 31, the box drive assembly 31 can drive the box assembly 32 to move in the horizontal direction, the box assembly 32 is provided with a placement slot 30, the box cover structure includes a box cover drive assembly 41 and a box cover assembly 42, the box cover drive assembly 41 is arranged on the frame 11, the box cover assembly 42 It is arranged at the output end of the box cover drive component 41 and is located above the box body component 32. The box cover drive component 41 can drive the box cover component 42 to move in the vertical direction so that the box cover component 42 is covered on the box body component 32. The box cover component 42 includes a box cover body 421. The box cover body 421 is provided with an air inlet hole group and an air extraction hole group. The air inlet hole group and the air extraction hole group are both connected to the air pump component. The chip structure 5 is arranged in the placement slot 30. The chip structure 5 is provided with a sample loading slot 501, a sample slot 502, a waste liquid slot 503 and a microchannel. The sample loading slot 501 is connected to the air inlet hole group, and the sample slot 502 and the waste liquid slot 503 are both connected to the air extraction hole group.

[0049] The single-cell nucleic acid processing instrument provided in this embodiment has a simple structure. The box body drive component 31 can drive the box body component 32 to move in the horizontal direction so that the box body component 32 is located directly below the box cover body 421 or the box body component 32 is pushed outward. The box cover drive component 41 can drive the box cover component 42 to move in the vertical direction so that the box cover body 421 is covered on the box body component 32. The degree of automation is higher, the experiment time is shorter, the experimental operation is simpler, and the customer satisfaction is higher.

[0050] Specifically, if Figure 3 As shown, the chip structure 5 of this embodiment includes a chip box 51 and two chip assemblies 52. Both chip assemblies 52 are disposed within the chip box 51, which is engaged within the placement slot 30. In other embodiments, the number of chip assemblies 52 included in the chip structure 5 is not limited to the two in this embodiment, and may also be one, three, or more than three, depending on actual needs. Each chip assembly 52 of this embodiment includes a reagent box, a chip body, and a sealing gasket. The sealing gasket and the chip body are respectively bonded to opposite sides of the reagent box.

[0051] Specifically, if Figure 4 As shown, a first card slot 510 is provided at one end of the chip box 51, and a card guide column 324 is provided on the box body assembly 32. A card elastic member 325 and a card block 326 are sleeved on the card guide column 324. One end of the card elastic member 325 is connected to the box body assembly 32, and the other end is connected to the card block 326. The card block 326 is slidably installed on the card guide column 324. The card block 326 can be carded in the card slot to fix the chip box 51 in the placement slot 30.

[0052] In order to better position the chip box 51, as Figure 4 As shown, the other end of the chip cassette 51 is further provided with a latching protrusion 511, and the cassette assembly 32 is provided with a second latching slot 320 corresponding to the latching protrusion 511. During installation, the latching protrusion 511 of the chip cassette 51 is first engaged with the second latching slot 320, so that one end of the chip cassette 51 is engaged with the cassette assembly 32. Then, an external force is applied to push the latching block 326 to compress the latching elastic member 325, so that the other end of the chip cassette 51 is placed in the placement slot 30. Finally, the latching block 326 is released. Under the action of the latching elastic member 325, the latching block 326 is pushed and engaged with the first latching slot 510, that is, the other end of the chip cassette 51 is engaged with the cassette assembly 32. This installation method can prevent the chip cassette 51 from tilting during the experiment, which could result in the sample loading slot 501 being unable to communicate with the air inlet group, and the sample slot 502 and waste liquid slot 503 being unable to communicate with the air extraction group, thereby increasing the success rate of the experiment.

[0053] It should be noted that if Figure 3As shown, the reagent kit of each chip assembly 52 in this embodiment is equipped with five sample loading slots 501, one sample slot 502, and one waste liquid slot 503. The sealing gasket is provided with seven through-holes, each corresponding to the five sample loading slots 501, one sample slot 502, and one waste liquid slot 503 on the reagent kit. The chip body is provided with microchannels that communicate with the five sample loading slots 501, one sample slot 502, and one waste liquid slot 503, respectively. The bottom of the microchannels is provided with a recessed pit. Accordingly, the lid body 421 is provided with two air inlet hole groups and two air extraction hole groups. Each air inlet hole group includes five air inlet holes 42102, which are respectively arranged in a one-to-one correspondence with the five sample loading slots 501 of a chip structure 5. Each air extraction hole group includes two air extraction holes, one of which is connected to the sample slot 502 of the chip structure 5, and the other is connected to the waste liquid slot 503 of the chip structure 5.

[0054] In other embodiments, the number of sample loading slots 501 on each chip assembly 52 is not limited to five as in this embodiment, and may be other numbers, depending on actual needs.

[0055] Specifically, if Figure 6 and Figure 10 As shown, the box cover assembly 42 of this embodiment also includes a first air intake control valve 422, a second air intake control valve (not shown in the figure), a first air extraction control valve 423, a second air extraction control valve 424, a third air extraction control valve 425 and a detection control valve 426. The first air intake control valve 422, the second air intake control valve, the first air extraction control valve 423, the second air extraction control valve 424, the third air extraction control valve 425 and the detection control valve 426 are all arranged on the box cover body 421.

[0056] In this embodiment, each air inlet 42102 is provided with a first air inlet control valve 422, and the first air inlet control valve 422 is configured to control the connection or disconnection between the air inlet 42102 and the air pump assembly. Figure 9 and Figure 12As shown, two air inlet channels 42101 are also provided on the box cover body 421. The two air inlet channels 42101 are arranged in a one-to-one correspondence with the two air inlet hole groups. One end of each air inlet channel 42101 is connected to the five air inlet holes 42102 of an air inlet hole group, and the other end is connected to the air pump assembly. The air inlet channel 42101, the air inlet hole group and the air extraction hole group of this embodiment are integrated on the box cover body 421. Compared with the prior art, the air inlet tube and the air extraction tube in the prior art are omitted. The air inlet hole group on the box cover body 421 is connected to the sample loading slot 501 on the chip structure 5. The first air inlet control valve 422 arranged at the air inlet hole 42102 can control the connection or disconnection of the air inlet hole 42102 and the air pump assembly. The air extraction hole group is connected to the waste liquid tank 503 and the sample collection tank on the chip structure 5. The first air extraction control valve 423 and the second air extraction control valve 424 arranged at the air extraction hole can control the connection or disconnection of the air extraction hole and the air pump assembly. The single-cell nucleic acid processing instrument has a simple structure and a high degree of air path integration, which is conducive to the modular setting of the single-cell nucleic acid processing instrument.

[0057] Furthermore, each air inlet hole 42102 of the present embodiment includes a first air inlet hole 42102 and a second air inlet hole 42102, the diameter of the second air inlet hole 42102 is smaller than the diameter of the first air inlet hole 42102, one end of the first air inlet control valve 422 extends into the first air inlet hole 42102 and forms a first upper air inlet cavity, a first middle air inlet cavity and a first bottom air inlet cavity with the air inlet hole 42102, the first middle air inlet cavity is located between the first upper air inlet cavity and the first bottom air inlet cavity, the air inlet channel 42101 is connected to the first middle air inlet cavity, and the second air inlet hole 42102 is connected to the first bottom air inlet cavity. When the first air inlet control valve 422 is powered off, the first upper air inlet cavity is connected to the first middle air inlet cavity, and the gas entering the first middle air inlet cavity through the air inlet channel 42101 can enter the first bottom air inlet cavity, and finally enter the sample loading tank 501 through the second air inlet hole 42102, thereby allowing the reagent in the sample loading tank 501 to pass into the microchannel.

[0058] like Figure 11As shown, the box cover body 421 of this embodiment is provided with two air intake blind holes 42105 and two first detection holes 42106, and each air intake blind hole 42105 is provided with a second air intake control valve (not shown in the figure), one end of the second air intake control valve extends into the air intake blind hole 42105 and forms a second upper air intake cavity, a second middle air intake cavity and a second bottom air intake cavity with the air intake blind hole 42105, the second middle air intake cavity is located between the second upper air intake cavity and the second bottom air intake cavity, each first detection hole 42106 is connected to a second upper air intake cavity, the second middle air intake cavity is connected to the air pump assembly, and the second bottom air intake cavity is connected to the air intake channel 42101, and the second air intake control valve is configured to connect the second upper air intake cavity and the second middle air intake cavity when the power is off, and to connect the second middle air intake cavity and the second bottom air intake cavity when the power is on.

[0059] Specifically, when the second air intake control valve is powered off, the second upper air intake chamber is connected to the second middle air intake chamber. At this time, the pressure of the gas entering the second middle air intake chamber from the air pump assembly can be detected through the first detection hole 42106; when the second air intake control valve is powered on, the second middle air intake chamber is connected to the second bottom air intake chamber. At this time, the gas discharged by the air pump assembly enters the second bottom air intake chamber through the second middle air intake chamber, and the gas in the second bottom air intake chamber enters the first middle air intake chamber through the air intake channel 42101.

[0060] like Figure 9 、 Figure 11 and Figure 12As shown, the two air extraction holes of each air extraction hole group of this embodiment are respectively a first air extraction hole 421041 and a second air extraction hole 421042. The first air extraction hole 421041 includes a first air extraction connecting hole 4210411 and a first connecting blind hole 4210412. The first air extraction connecting hole 4210411 is connected to the waste liquid tank 503. The second air extraction hole 421042 includes a second air extraction connecting hole 4210421 and a second connecting blind hole 4210422. The second air extraction connecting hole 4210421 is connected to the sample receiving tank. The first connecting blind hole 4210412 A first air extraction control valve 423 is provided inside, and the first air extraction control valve 423 and the first connecting blind hole 4210412 form a first upper connecting cavity, a first middle connecting cavity and a first bottom connecting cavity. A second detection hole 42107 is provided on the box cover body 421, and the second detection hole 42107 is connected with the first bottom connecting cavity, and the first middle connecting cavity is connected with the first air extraction connecting hole 4210411. The first air extraction control valve 423 is configured to connect the first upper connecting cavity and the first middle connecting cavity when the power is off, and connect the first middle connecting cavity with the first bottom connecting cavity when the power is on. A second air extraction control valve 424 is arranged in the second connecting blind hole 4210422. The second air extraction control valve 424 and the second connecting blind hole 4210422 form a second upper connecting chamber, a second middle connecting chamber and a second bottom connecting chamber. The second middle connecting chamber is located between the second upper connecting chamber and the second bottom connecting chamber. The second upper connecting chamber is connected to the second air extraction connecting hole 4210421, the second middle connecting chamber is connected to the air extraction channel 42103, and the second bottom connecting chamber is connected to the first upper connecting chamber. The second air extraction control valve 424 is configured to connect the second upper connecting chamber and the second middle connecting chamber when the power is off, and to connect the second middle connecting chamber and the second bottom connecting chamber when the power is on.

[0061] Specifically, when the first air extraction control valve 423 is powered on and the second air extraction control valve 424 is powered on, the first upper connecting chamber is connected to the first middle connecting chamber and the first middle connecting chamber is connected to the first air extraction connecting hole 4210411, which is equivalent to extracting air from the waste liquid tank 503. Since the second air extraction control valve 424 is powered on, the second middle connecting chamber is connected to the second bottom connecting chamber and the air extraction channel 42103 is connected to the second middle connecting chamber. Since the second bottom connecting chamber is connected to the first upper connecting chamber, the air pump assembly can extract the gas in the waste liquid tank 503 through the first air extraction connecting hole 4210411, the first middle connecting chamber, the first upper connecting chamber, the second bottom connecting chamber, and the second middle connecting chamber to the air extraction channel 42103 in sequence.

[0062] When the first air extraction control valve 423 is energized, the second bottom connecting chamber is no longer connected to the first upper connecting chamber, that is, the air pump assembly cannot continue to extract the gas in the waste liquid tank 503, so that the first middle connecting chamber is connected to the first bottom connecting chamber, the first middle connecting chamber is connected to the first air extraction connecting hole 4210411, the first air extraction connecting hole 4210411 is connected to the waste liquid tank 503 and the second detection hole 42107 is connected to the first bottom connecting chamber. Therefore, the pressure of the gas in the waste liquid tank 503 can be detected through the second detection hole 42107.

[0063] When the second air extraction control valve 424 is powered off, the second upper connecting chamber is connected to the second middle connecting chamber, the air extraction channel 42103 is connected to the second middle connecting chamber, and the second upper connecting chamber is connected to the second air extraction connecting hole 4210421. Since the second middle connecting chamber is not connected to the second bottom connecting chamber, the air pump assembly cannot extract the gas in the waste liquid tank 503. At this time, the air pump assembly can extract the gas in the sample collecting tank through the second air extraction connecting hole 4210421, the second upper connecting chamber, and the second middle connecting chamber to the air extraction channel 42103 in sequence.

[0064] like Figure 7 、 Figure 9 、 Figure 11 and Figure 12 As shown, the box cover body 421 of this embodiment is provided with two air extraction blind holes 42108 and two third detection holes 42109, and each air extraction blind hole 42108 is provided with a third air extraction control valve 425, one end of the third air extraction control valve 425 extends into the air extraction blind hole 42108 and forms an upper air extraction cavity, a middle air extraction cavity and a bottom air extraction cavity with the air extraction blind hole 42108, the middle air extraction cavity is located between the upper air extraction cavity and the bottom air extraction cavity, a third detection hole 42109 is connected to an upper air extraction cavity, the middle air extraction cavity is connected to the air pump assembly, and the bottom air extraction cavity is connected to the air extraction channel 42103, and the third air extraction control valve 425 is configured to connect the upper air extraction cavity and the middle air extraction cavity when the power is off, and connect the middle air extraction cavity with the bottom air extraction cavity when the power is on.

[0065] Specifically, when the third air pumping control valve 425 is powered off, the upper air pumping chamber is connected to the middle air pumping chamber, and the air pump assembly is connected to the upper air pumping chamber through the middle air pumping chamber. At this time, the pressure of the gas in the upper air pumping chamber can be detected through the third detection hole 42109; when the third air pumping control valve 425 is powered on, the middle air pumping chamber is connected to the bottom air pumping chamber. At this time, the air pump assembly extracts the gas in the air pumping channel 42103 through the middle air pumping chamber and the bottom air pumping chamber.

[0066] like Figure 6 、 Figure 7 、 Figure 9 、 Figure 11 and Figure 12As shown, the box cover body 421 of this embodiment is further provided with two detection blind holes 421010 and two fourth detection holes 421011, and each air inlet channel 42101 corresponds to a detection blind hole 421010 and a fourth detection hole 421011. Figure 6 As shown, a detection control valve 426 is provided at each detection blind hole 421010, and each detection control valve 426 and the detection blind hole 421010 form a middle detection chamber and a bottom detection chamber, the bottom detection chamber is connected to the fourth detection hole 421011, and the middle detection chamber is connected to the air inlet channel 42101. The detection control valve 426 is configured to control the connection or disconnection between the middle detection chamber and the bottom detection chamber.

[0067] Specifically, when the detection control valve 426 is opened, the middle detection chamber and the bottom detection chamber are connected, and the pressure of the gas in the bottom detection chamber can be detected through the fourth detection hole 421011; when the detection control valve 426 is closed, the middle detection chamber is not connected with the bottom detection chamber, and the pressure of the gas in the bottom detection chamber cannot be detected through the fourth detection hole 421011.

[0068] like Figure 6 and Figure 10 As shown, the box cover assembly 42 of this embodiment also includes a circuit board 427, which is electrically connected to ten first air intake control valves 422, two second air intake control valves, two first air exhaust control valves 423, two second air exhaust control valves 424, two third air exhaust control valves 425 and two detection control valves 426.

[0069] When the single-cell nucleic acid processing instrument of this embodiment is used to introduce gas into the sample loading tank 501, the first air inlet control valve 422 is energized and the second air inlet control valve is energized. At this time, the gas discharged from the air pump assembly enters the second bottom air inlet cavity through the second middle air inlet cavity, and the gas in the second bottom air inlet cavity enters the first middle air inlet cavity through the air inlet channel 42101. The gas in the first middle air inlet cavity can enter the first bottom air inlet cavity and finally enter the sample loading tank 501 through the second air inlet hole 42102, thereby allowing the reagent in the sample loading tank 501 to pass into the microchannel.

[0070] When the single-cell nucleic acid processing instrument of this embodiment is used to extract the gas in the waste liquid tank 503, the first air extraction control valve 423 is powered on and the second air extraction control valve 424 is powered on. The air pump assembly can extract the gas in the waste liquid tank 503 to the air extraction channel 42103 through the first air extraction connecting hole 4210411, the first middle connecting cavity, the first upper connecting cavity, the second bottom connecting cavity, and the second middle connecting cavity in sequence.

[0071] When the single-cell nucleic acid processing instrument of this embodiment is used to extract the gas in the sample collection tank, the second gas extraction control valve 424 is powered off, and the air pump assembly can extract the gas in the sample collection tank to the gas extraction channel 42103 through the second gas extraction connecting hole 4210421, the second upper connecting cavity, and the second middle connecting cavity in sequence.

[0072] like Figures 12 to 15 As shown, the single-cell nucleic acid processing instrument of this embodiment also includes an air path integrated block 61, on which are provided two first connecting channels 6101, two second connecting channels 6102 and two first detection channels 6103. One end of each first connecting channel 6101 is connected to the air pump assembly, and the other end of the first connecting channel 6101 is connected to the same end of a second connecting channel 6102 and a first detection channel 6103. The other end of the second connecting channel 6102 is connected to an air inlet channel 42101 through a connecting joint 62 and a connecting tube 63, and the other end of the first detection channel 6103 is connected to a first pressure detector. The air path integrated block 61 is also provided with two third connecting channels 6104, two fourth connecting channels 6105 and two second detection channels 6106. One end of each third connecting channel 6104 is connected to the air extraction channel 42103 through a connecting joint 62 and a connecting pipe 63, and the other end of the third connecting channel 6104 is connected to the same end of a fourth connecting channel 6105 and a second detection channel 6106. The other end of the fourth connecting channel 6105 is connected to the air pump assembly, and the other end of the second detection channel 6106 is connected to the second pressure detector.

[0073] like Figure 12 and Figure 15 As shown, the single-cell nucleic acid processing instrument of this embodiment also includes an adjusting block 64 and an adjusting member 65. The adjusting block 64 is fixed on the integrated block, and the connecting pipe 63 is set through the adjusting block 64. The adjusting member 65 is set on the adjusting block 64 and can abut against the connecting pipe 63. The adjusting member 65 is a bolt. By adjusting the depth of the bolt screwed into the adjusting block 64, the flow rate of gas flowing through the connecting pipe 63 per unit time can be changed.

[0074] In other embodiments, the other end of the first detection channel 6103 is not provided with a first pressure detector, but is instead interference-fitted with a first plug. The other end of the second detection channel 6106 is not provided with a second pressure detector, but is instead interference-fitted with a second plug. When it is necessary to detect the pressure within the first connecting channel 6101 and the second connecting channel 6102, the first plug is removed and an external pressure detector is connected to the first detection channel 6103. When it is necessary to detect the pressure within the third connecting channel 6104 and the fourth connecting channel 6105, the second plug is removed and the external pressure detector is connected to the second detection channel 6106.

[0075] In the prior art, in order to detect the gas pressure entering the two air intake channels 42101, an air intake tee is provided between each air intake channel 42101 and the air pump assembly. In order to detect the gas pressure entering the two air exhaust channels 42103, an air exhaust tee is provided between each air exhaust channel 42103 and the air pump assembly. The air path integrated block 61 of this embodiment replaces the existing two air intake tees and two air exhaust tees, and the structure is simpler and more compact.

[0076] like Figure 1 As shown, the number of air pump components in this embodiment is two, and each air pump component includes an air pump motor, an air pump ball screw, an air pump body 21 and an air pump transmission component 22. The air pump motor is arranged on the frame 11, and the air pump transmission component 22 includes a first air pump gear 221, a second air pump gear 222 and an air pump conveyor belt 223. The first air pump gear 221 is arranged at the output end of the air pump motor, and the air pump conveyor belt 223 is arranged on the first air pump gear 221 and the second air pump gear 222. The air pump motor can drive the first air pump gear 221 to rotate The air pump gear 222 rotates synchronously therewith, and the air pump ball screw includes an air pump screw and an air pump nut. The air pump screw is fixedly connected to the second air pump gear 222, and the air pump nut is threadedly connected to the air pump screw. The air pump body 21 includes an air pump barrel 211 and an air pump push rod assembly 212. The air pump barrel 211 is arranged on the frame 11 and defines an air pump cavity therein. The air pump cavity can be connected to the air inlet hole group and the air extraction hole group respectively. One end of the air pump push rod assembly 212 is sealed and slidably connected to the air pump barrel 211, and the other end is fixedly connected to the air pump nut. Specifically, the air pump push rod assembly 212 includes a push rod body and a piston. One end of the push rod body is fixedly connected to the air pump nut, and the other end is fixedly connected to the piston. The piston is sealed and slidably connected to the air pump barrel 211.

[0077] like Figure 1 As shown, the frame 11 of this embodiment includes a mounting plate 11, the air pump motor is arranged on the lower side of the mounting plate 11, and the air pump transmission assembly 22 is arranged on the upper side of the mounting plate 11, so that the total height of the air pump assembly is lower and the structure is more compact, which is conducive to the miniaturization of the single-cell nucleic acid processing instrument.

[0078] Since the air pump body 21 is controlled by an air pump ball screw, its movement accuracy is higher, and accurate micro-feeding of the air pump push rod assembly 212 is achieved. When the reagent in the sample loading tank 501 needs to be added to the microchannel, the sample loading tank 501 is inflated, and the air pump motor drives the air pump screw to rotate so that the air pump nut drives the air pump push rod assembly 212 to move in a vertical downward direction. At this time, the gas in the air pump barrel 211 is sequentially pushed into the first connecting channel 6101, the second connecting channel 6102, the air inlet channel 42101, one of the air inlet holes 42102 and finally into the sample loading tank 501, thereby pushing the reagent in the sample loading tank 501 to flow and enter the microchannel; when When it is necessary to evacuate the waste liquid tank 503 so that the reagent in the microchannel enters the waste liquid tank 503, the air pump motor drives the air pump screw to rotate so that the air pump nut drives the air pump push rod assembly 212 to move in a vertical upward direction. At this time, the gas in the waste liquid tank 503 enters the air pump barrel 211 through the air extraction hole, the air extraction channel 42103, the fourth connecting channel 6105 and the third connecting channel 6104 in turn. Similarly, when evacuating the sample tank 502, the air extraction process is similar to the process of extracting the gas in the waste liquid tank 503.

[0079] like Figure 1 and Figure 2 As shown, the box cover drive assembly 41 of this embodiment includes two box cover motor assemblies, and the two box cover motor assemblies are respectively located on the left and right sides of the box cover body 421. Each box cover motor assembly includes a box cover motor body 411 and a box cover ball screw (not shown in the figure). The box cover motor body 411 is arranged on the frame 11, and the box cover ball screw includes a box cover screw and a box cover nut. The box cover screw is arranged at the output end of the box cover motor body 411, and the box cover nut is threadedly connected to the box cover screw, and the box cover nut is fixedly connected to the box cover body 421.

[0080] Specifically, if Figure 1 As shown, the frame 11 of this embodiment includes a placement plate 12, and the cover motor body 411 is arranged on the lower side of the placement plate 12, as shown in FIG. Figure 1 As shown, the box cover driving assembly 41 further includes two box cover conveying assemblies 412, and the box cover conveying assemblies 412 are arranged on the upper side of the placement plate 12, as shown in FIG. Figure 1As shown, each lid conveying assembly 412 includes a first lid gear 4121, a second lid gear 4122, and a lid conveyor belt 4123. The first lid gear 4121 is fixedly mounted at the output end of the lid motor body 411, the second lid gear 4122 is fixedly connected to the lid screw, and the lid conveyor belt 4123 engages with the first lid gear 4121 and the second lid gear 4122. The lid motor body 411 is positioned below the placement plate 12, and the lid conveying assembly 412 is positioned above the placement plate 12. This reduces the overall height of the lid drive assembly 41 and makes the structure more compact, facilitating the miniaturization of the single-cell nucleic acid processing instrument.

[0081] In other embodiments, if there is no limit on the height of the single-cell nucleic acid processing instrument, the box cover drive assembly 41 may also not include the box cover conveying assembly 412. In this case, the box cover motor body 411 is set on the upper side of the placement plate 12, and the box cover screw is directly set at the output end of the box cover motor body 411.

[0082] The lid motor bodies 411 of the two lid motor assemblies of this embodiment can simultaneously drive the lid ball screws to drive the lid body 421 to move upward or downward in the vertical direction, so that the lid body 421 is covered on the box body assembly 32. In the prior art, in order to achieve the vertical movement of the lid body 421, the two lid drive assemblies 41 are two cylinder drive structures. When the cylinder drive structures drive the lid body 421, there are often different movement distances on both sides of the lid body 421, which makes it impossible for the lid body 421 to fully engage with the box body assembly 32, resulting in gaps between the air inlet holes 42102 of the air inlet hole group and the sample loading slot 501 on the chip structure 5, or gaps between the air extraction holes of the air extraction hole group and the sample slot 502 and waste liquid slot 503 on the chip structure 5, which is not conducive to the conduct of the entire experiment.

[0083] Compared with the existing cylinder-driven slider that drives the box cover body 421 to move, the box cover drive assembly 41 of this embodiment can make the box cover body 421 move in the vertical direction with higher precision, thereby achieving precise micro-feeding of the box cover body 421, so that the box cover body 421 is fully engaged with the box body assembly 32.

[0084] like Figure 2As shown, the box drive assembly 31 of this embodiment includes a first box motor 311 and a box ball screw 312. The first box motor 311 is disposed on the frame 11. The box ball screw 312 includes a box screw and a box nut. The box screw is disposed at the output end of the first box motor 311. The box nut is threadedly connected to the box screw and is fixedly connected to the box assembly 32. The first box motor 311 can drive the box screw to rotate so that the box nut drives the box assembly 32 to move horizontally. The first box motor 311 and the box ball screw 312 can move the box assembly 32 horizontally with high precision, achieving precise micro-feeding of the box assembly 32.

[0085] In other embodiments, the box body drive assembly 31 includes a second box body motor and a box body gear transmission assembly, the second box body motor is arranged on the frame 11, the box body gear transmission assembly includes a meshing box body gear and a box body rack, the box body gear is fixedly set at the output end of the second box body motor, the box body rack is slidably set on the frame 11 and fixedly connected to the box body assembly 32, and the second box body motor can drive the box body gear to rotate so that the box body rack drives the box body assembly 32 to move in the horizontal direction.

[0086] like Figure 5 As shown, the box assembly 32 of this embodiment includes a box body 321, a heat preservation frame 322 and a heat conducting assembly 323. The heat conducting assembly 323 and the box body 321 form a chamber with an open top, as shown in FIG. Figure 5 As shown, the heat conducting assembly 323 includes a heat conducting member 3231, a Peltier (not shown in the figure) and a heat sink 3232 stacked in sequence. A cooling fan (not shown in the figure) is provided on the rack 11. The heat conducting member 3231 is provided on the box assembly 32 and the two form a chamber with an open top. The Peltier is located below the heat conducting member 3231 and can heat and cool the chamber. The Peltier is provided on the side of the heat sink 3232 close to the heat conducting member 3231 or on the heat conducting member 32 31 is close to one side of the heat sink 3232, the heat sink 3232 is located below the Peltier, the insulation frame 322 is arranged along the circumference of the heat conductor 3231 and the insulation frame 322 is clamped between the heat conductor 3231 and the heat sink 3232, and the insulation frame 322 is provided with an avoidance hole 3220, the avoidance hole 3220 is arranged opposite to the heat conductor 3231 and the heat sink 3232, and the cooling fan is fixedly arranged on the frame 11 and the cooling fan can be arranged opposite to the heat sink 3232.

[0087] The heat conducting member 3231 of this embodiment is a copper block, which has a good thermal conductivity. Specifically, when the chamber is heated, the side of the Peltier close to the copper block generates heat, and the copper block can quickly transfer the heat generated by the Peltier into the chamber, thereby heating the chamber; when the chamber is cooled, the side of the Peltier close to the copper block is cooled, and the copper block can quickly transfer the heat in the chamber to the Peltier, thereby cooling the chamber. The placement slot 30 is provided on the copper block. The placement slot 30 of this embodiment can simultaneously place two chip structures 5. In other embodiments, the heat conducting member 3231 is not limited to the copper block of this embodiment. The heat conducting member 3231 can also be made of other materials with good thermal conductivity. The placement slot 30 on the heat conducting member 3231 can also place one or at least three chip structures 5 at the same time.

[0088] like Figure 5 As shown, the heat sink 3232 of this embodiment is a heat sink fin, and the Peltier is arranged on the side of the heat sink fin close to the heat conductor 3231. Specifically, when the chamber needs to be cooled, the Peltier is connected to the power supply in the forward direction. The temperature of the side of the Peltier close to the chamber decreases, and the heat conductor 3231 quickly transfers the heat in the chamber to the Peltier. The temperature of the side of the Peltier facing away from the chamber increases, and the heat sink fin absorbs the heat of the Peltier, thereby timely cooling the Peltier. When the chamber needs to be heated, the Peltier is connected to the power supply in the reverse direction, that is, the direction of the current is switched. At this time, the temperature of the side of the Peltier close to the chamber increases, and the temperature of the side facing away from the chamber decreases. At this time, the Peltier heats the chamber through the heat conductor 3231. In other embodiments, the installation position of the Peltier is not limited to that of this embodiment, and can also be arranged on the side of the heat conductor 3231 close to the heat sink fin.

[0089] The box body assembly 32 of this embodiment also includes a temperature sensor (not shown in the figure), a first temperature switch (not shown in the figure) and a second temperature switch (not shown in the figure). The temperature sensor is used to measure the temperature of the heat conductor 3231. The first temperature switch and the second temperature switch are arranged in series. The first temperature switch can measure the temperature of the heat sink 3232 and is configured to disconnect when the temperature of the heat sink 3232 reaches a first preset temperature to disconnect the Peltier from the power supply and stop heating the chip structure 5. The second temperature switch can measure the temperature of the heat conductor 3231 and is configured to disconnect when the temperature of the heat conductor 3231 reaches a second preset temperature to disconnect the Peltier from the power supply and stop heating the chip structure 5. The second preset temperature is higher than the first preset temperature.

[0090] Specifically, when heating the chip structure 5, the Peltier is energized, and after being energized, the Peltier can heat the heat conductor 3231. When the first temperature switch measures that the temperature of the heat sink 3232 exceeds the first preset temperature, the first temperature switch is disconnected to disconnect the Peltier from the power supply and stop heating the chip structure 5; or when the second temperature switch detects that the temperature of the heat conductor 3231 reaches the second preset temperature, the second temperature switch is disconnected to disconnect the Peltier from the power supply and stop heating the chip structure 5.

[0091] When the temperature sensor cannot normally feedback the real-time temperature of the heat sink 3232, the temperatures of the heat conductor 3231 and the heat sink 3232 continue to rise. When the first temperature switch measures that the temperature of the heat sink 3232 exceeds the first preset temperature or the second temperature switch detects that the temperature of the heat conductor 3231 is higher than the second preset temperature, the Peltier is disconnected from the power supply and stops heating the chip structure 5.

[0092] In other embodiments, the heat sink 3232 is a heat sink having a cooling channel disposed therein. Cooling water in the cooling channel can cool the heat sink. In this case, the single-cell nucleic acid processing instrument further includes a water chiller, the outlet of which is connected to the inlet of the cooling channel, and the inlet of which is connected to the outlet of the cooling channel. Specifically, the water entering the water chiller is high-temperature water discharged from the heat sink. The water chiller can cool this high-temperature water to low-temperature water, which is then discharged from the outlet of the water chiller and reenters the heat sink to absorb heat.

[0093] The single cell nucleic acid processing instrument of this embodiment also includes a magnet feeding structure, such as Figure 16 and Figure 17 As shown, the magnet feeding structure includes a magnet driving component 71, a connecting component 72 and a magnet arm 73. The magnet driving component 71 is arranged on the frame 11, and the connecting component 72 is arranged at the output end of the magnet driving component 71. Figure 16 As shown, the connecting component 72 includes a first connecting block 721, a second connecting block 722 and a feed elastic member 723, the feed elastic member 723 is a feed spring, the second connecting block 722 is slidably set on the first connecting block 721, and the feed elastic member 723 is clamped between the first connecting block 721 and the second connecting block 722. When the first connecting block 721 is powered on, it can adsorb the second connecting block 722, and when the power is off, the feed elastic member 723 can reset the second connecting block 722. The magnet arm 73 is fixedly set on the second connecting block 722, and the magnet driving component 71 can drive the connecting component 72 to drive the magnet arm 73 to move in the horizontal direction so that the magnet arm 73 is facing the chip structure 5. When the first connecting block 721 is powered on, the magnet arm 73 can move in the vertical direction toward the direction close to the chip structure 5 with the second connecting block 722.

[0094] The magnet driving assembly 71 of the magnet feeding structure provided in this embodiment can drive the magnet arm 73 to move in the horizontal direction so that the magnet arm 73 is facing the chip structure 5. After the first connecting block 721 is powered on, it can adsorb the second connecting block 722 to make the second connecting block 722 move in the vertical direction toward the chip structure 5, so that the magnet arm 73 is closer to the chip structure 5, so that the magnet arm 73 can better adsorb the cell-carrying magnetic beads, increasing the probability of the cell-carrying magnetic beads escaping from the pit at the bottom of the microchannel. The additional feeding elastic member 723 can reset the second connecting block 722 when the first connecting block 721 is powered off.

[0095] Specifically, if Figure 16 As shown, the connecting assembly 72 of this embodiment further includes a feed guide post 724 extending in the vertical direction. One end of the feed guide post 724 extends into the first connecting block 721, and the other end extends into the second connecting block 722. The feed elastic member 723 is sleeved on the feed guide post 724. The additional feed guide post 724 can attract the second connecting block 722 when the first connecting block 721 is powered on, allowing the second connecting block 722 to move in the vertical direction. It can also enable the second connecting block 722 to move in the vertical direction when the feed elastic member 723 resets the second connecting block 722 when the first connecting block 721 is powered off. Furthermore, in this embodiment, there are four feed guide posts 724, which are respectively located at the four corners of the first connecting block 721. There are four feed elastic members 723, which are arranged in a one-to-one correspondence with each other, and each feed elastic member 723 is sleeved on a feed guide post 724. In other embodiments, the number of feed guide posts 724 and feed elastic members 723 is not limited to four in this embodiment, and may also be one, two, three, or more than four, depending on actual needs.

[0096] Specifically, the first connecting block 721 of this embodiment is an electromagnet, and the second connecting block 722 is an iron block. When the electromagnet is energized, it can generate an electromagnet to attract the iron block so that the iron block drives the magnet arm 73 to move in the vertical direction. The magnet arm 73 moves toward the direction close to the chip structure 5, and the feed elastic member 723 is compressed. When the electromagnet is de-energized, the magnetism of the electromagnet disappears, and the force between the electromagnet and the iron block disappears. The feed elastic member 723 pushes the second connecting block 722 to move in the vertical direction away from the chip structure 5, so that the iron block is reset to its initial position. Furthermore, when the first connecting block 721 of this embodiment is energized, the second connecting block 722 can move 1 mm in the vertical direction toward the direction close to the first connecting block 721, that is, the feed elastic member 723 is compressed 1 mm. When the second connecting block 722 is de-energized, the feed elastic member 723 can push the second connecting block 722 to reset. In other embodiments, the distance that the feed elastic member 723 is compressed when the first connecting block 721 is powered on is not limited to 1 mm in this embodiment, and can also be other distances, which are specifically set according to actual needs.

[0097] like Figure 16 and Figure 17 As shown, the magnet drive assembly 71 of this embodiment includes a feed motor 711 and a feed ball screw 712. The feed motor 711 is a rotary motor. The output end of the feed motor 711 is connected to the feed ball screw 712. The first connecting block 721 is provided on the feed ball screw 712. The feed motor 711 and the feed ball screw 712 can move the magnet arm 73 in the horizontal direction with high precision, thereby achieving precise micro-feeding of the magnet arm 73. Specifically, the feed ball screw 712 includes a feed screw and a feed thread block. The feed thread block is threadedly connected to the feed screw. The connecting assembly 72 is fixedly provided on the feed thread block. The feed motor 711 can drive the feed screw to rotate so that the feed thread block drives the connecting assembly 72 to move in the horizontal direction.

[0098] In order to ensure that the feed screw block can move linearly in the horizontal direction without rotating relative to the frame 11, the frame 11 of this embodiment is provided with two feed slide rails (not shown in the figure) extending in the horizontal direction, and the feed screw block is provided with two feed slide grooves (not shown in the figure) corresponding to the feed slide rails, and the feed screw block is slidably connected to the frame 11.

[0099] In this embodiment, the magnet arm 73 is provided with two escape slots 730, which are arranged in parallel and extend through the magnet arm 73. The additional escape slots 730 prevent the magnet arm 73 from squeezing and damaging the chip structure 5, ensuring that the magnet arm 73 can move vertically toward the chip structure 5 while remaining spaced apart from the chip structure 5.

[0100] Preferably, the single-cell nucleic acid processing instrument of this embodiment also includes a controller, which is electrically connected to the air pump assembly, the box body drive assembly 31, the box cover drive assembly 41, the Peltier, the first connecting block 721 and the feed motor 721 respectively. The controller can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a distributed multi-chip microcontroller. The control program can be run in the microcontroller to control the air pump assembly, the box body drive assembly 31, the box cover drive assembly 41, the Peltier, the first connecting block 721 and the feed motor 721 to realize their functions.

[0101] When using the single-cell nucleic acid processing instrument of this embodiment to extract RNA from cells, the specific operating steps are as follows:

[0102] Step 1: Start the cover motor body 411, which drives the cover ball screw and the cover transmission assembly 412 to move the cover assembly 42 upward in the vertical direction, and the cover assembly 42 is separated from the box body assembly 32;

[0103] Step 2: simultaneously start the two first box body motors 311, and the first box body motors 311 drive the box body ball screw 312 to drive the box body assembly 32 to extend outward in the horizontal direction;

[0104] Step 3: The experimenter clamps the chip structure 5 onto the box assembly 32;

[0105] Step 4: Add the first reagent, solution with cells, solution with magnetic beads, second reagent, and third reagent required for the experiment into the five sample loading slots 501 of each chip assembly 52 respectively;

[0106] Step 5: Simultaneously start the two first box body motors 311. The first box body motors 311 drive the box body ball screw 312 to drive the box body assembly 32 to reset horizontally to just below the box cover assembly 42.

[0107] Step 6: Start the cover motor body 411, which drives the cover ball screw and the cover transmission assembly 412 to move the cover assembly 42 downward in the vertical direction, so that the cover assembly 42 fits with the box body assembly 32;

[0108] Step 7: Start the air pump motor and energize the first air inlet control valve 422 that controls the air flow to the sample loading reservoir 501 containing the first reagent. Air enters the sample loading reservoir 501 containing the first reagent, and the first reagent is pressed into the chip body. Then, the first air inlet control valve 422, which is energized in this step, is de-energized, thereby stopping the flow of the first reagent.

[0109] Step 8: Start the air pump motor and energize the first air extraction control valve 423 that controls the air flow to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the waste liquid tank 503, thereby driving the flow of liquid in the microchannel. The first reagent cleans the microchannel. Then, the first air extraction control valve 423, which has been energized in this step, is de-energized, thereby stopping the flow of the solution in the microchannel.

[0110] Step 9: The first air inlet control valve 422, which controls the air flow to the sample loading tank 501 containing the cell-containing solution, is energized. Air enters the sample loading tank 501 containing the cell-containing solution, and the cell-containing solution is pressed into the chip body. The first air inlet control valve 422, which is energized in this step, is then de-energized, thereby stopping the flow of the cell-containing solution.

[0111] Step 10: Start the air pump motor and energize the first air extraction control valve 423 that controls the air passage to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the waste liquid tank 503, thereby driving the flow of liquid in the microchannel and pumping the cell-containing solution into the microchannel. The first air extraction control valve 423, which was energized in this step, is then de-energized, thereby stopping the flow of the solution in the microchannel.

[0112] Step 11: Allow a certain amount of time for the cells to settle by gravity and fall into the pit at the bottom of the microchannel of the chip body;

[0113] Step 12: Start the air pump motor and energize the first air inlet control valve 422 that controls the air flow to the sample loading reservoir 501 containing the first reagent. Air enters the sample loading reservoir 501 containing the first reagent, and the first reagent is pressed into the chip body. Then, the first air inlet control valve 422, which was energized in this step, is de-energized, thereby stopping the flow of the first reagent.

[0114] Step 13: Start the air pump motor and energize the first air extraction control valve 423 that controls the air flow to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the waste liquid tank 503, thereby driving the flow of liquid in the microchannel. This pumps the first reagent into the microchannel, flushes away excess cells, and leaves only an appropriate amount of cells in the chip body. The first air extraction control valve 423, which was energized in this step, is then de-energized, thereby stopping the flow of the solution in the microchannel.

[0115] In step 14, the first air inlet control valve 422 that controls the air flow to the sample loading tank 501 containing the solution containing magnetic beads is energized. Air enters the sample loading tank 501 containing the solution containing magnetic beads, and the solution containing magnetic beads is pressed into the chip body. The first air inlet control valve 422 energized in this step is then de-energized, thereby stopping the flow of the solution containing magnetic beads.

[0116] Step 15: Start the air pump motor and energize the first air extraction control valve 423 that controls the air passage to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the waste liquid tank 503, thereby driving the flow of liquid in the microchannel and pumping the solution containing the magnetic beads into the microchannel. The first air extraction control valve 423, which was energized in this step, is then de-energized, thereby stopping the flow of the solution in the microchannel.

[0117] Step 16: Start the air pump motor and energize the first air inlet control valve 422 that controls the air flow to the sample loading reservoir 501 containing the first reagent. Air enters the sample loading reservoir 501 containing the first reagent, and the first reagent is pressed into the chip body. Then, the first air inlet control valve 422, which was energized in this step, is de-energized, thereby stopping the flow of the first reagent.

[0118] Step 17: Start the air pump motor and energize the first air extraction control valve 423 that controls the air flow to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the waste liquid tank 503, thereby driving the flow of liquid in the microchannel. The first reagent is pumped into the microchannel, and the excess magnetic beads are flushed, leaving only an appropriate amount of magnetic beads in the chip body. Then, the first air extraction control valve 423, which was energized in this step, is de-energized, thereby stopping the flow of the solution in the microchannel.

[0119] In step 18, the first air inlet control valve 422, which controls the air passage to the sample loading reservoir 501 containing the second reagent, is energized. Gas enters the sample loading reservoir 501 containing the second reagent, and the second reagent is pressed into the chip body. The first air inlet control valve 422, which was energized in this step, is then de-energized, thereby stopping the flow of the second reagent.

[0120] Step 19: Start the air pump motor and energize the first air extraction control valve 423 that controls the air passage to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the waste liquid tank 503, thereby driving the flow of liquid in the microchannel. The second reagent is then drawn into the microchannel to initiate a biochemical reaction, allowing the cellular RNA to combine with the molecular structure on the surface of the magnetic beads to form RNA with magnetic beads. The first air extraction control valve 423, which was energized in this step, is then de-energized, thereby stopping the flow of the solution in the microchannel.

[0121] Step 20: Start the feed motor 711, which drives the magnet arm 73 to extend directly above the chip assembly 52. ​​Then, the first connecting block 721 is electrified. The first connecting block 721 attracts the second connecting block 722, causing the magnet arm 73 to move vertically toward the chip assembly 52. ​​The magnet arm 73 attracts the RNA with magnetic beads in the microchannel, causing it to suspend in the microchannel.

[0122] Step 21: De-energize the first connecting block 721, reset the second connecting block 722 and the magnet arm 73 in the vertical direction by the feed elastic member 723, start the feed motor 711, and reset the magnet arm 73 in the horizontal direction;

[0123] In step 22, the first air inlet control valve 422 that controls the air passage to the sample loading reservoir 501 containing the third reagent is energized, and gas enters the sample loading reservoir 501 containing the third reagent. The third reagent is pressed into the chip body, and then the first air inlet control valve 422 energized in this step is de-energized, thereby stopping the flow of the third reagent.

[0124] Step 23: Start the air pump motor and energize the second air extraction control valve 424 that controls the air flow to the waste liquid tank 503 containing the cell-containing solution. This changes the air pressure in the sample collection tank, thereby driving the flow of liquid in the microchannel, pumping the third reagent into the microchannel, and collecting the RNA with magnetic beads in the sample collection tank. Then, de-energize the second air extraction control valve 424 that was energized in this step, thereby stopping the flow of the solution in the microchannel.

[0125] Step 24: Start the cover motor body 411, which drives the cover ball screw and the cover transmission assembly 412 to move the cover assembly 42 upward in the vertical direction, and the cover assembly 42 is separated from the box body assembly 32;

[0126] Step 25: Simultaneously start the two first box body motors 311. The first box body motors 311 drive the box body ball screw 312 to drive the box body assembly 32 to extend outward in the horizontal direction.

[0127] Step 26: Take the RNA with magnetic beads out of the sample collection tank and put the chip structure 5 into a designated waste collection box;

[0128] Step 27: simultaneously start the two first box body motors 311, and the first box body motors 311 drive the box body ball screw 312 to drive the box body assembly 32 to reset horizontally to just below the box cover assembly 42;

[0129] Step 28, start the box cover motor body 411, the box cover motor body 411 drives the box cover ball screw and the box cover transmission assembly 412 to drive the box cover assembly 42 to move downward in the vertical direction, and the box cover assembly 42 is fitted with the box body assembly 32. At this point, the entire experiment is completed.

[0130] It should be noted that in each of the above steps, in order to meet the temperature requirements of different reagents and the temperature requirements of biochemical reactions, the chip assembly 52 and the chamber are heated or cooled by adjusting the heat conductive component 323. Specifically, the reagents in the chip assembly 52 are heated by the Peltier and heat conductive component 3231, and the reagents in the chip assembly 52 are cooled by the heat dissipation component 3232 and the cooling fan. The specific temperature adjustment process is not reflected in the above process. In actual operation, the appropriate temperature required for each step can be achieved by programming in the controller, while the temperature sensor detects the temperature of the heat conductive component 3231 in real time to control the temperature of the reagents. When the single-cell nucleic acid processing instrument is in operation, the cooling fan is always on to dissipate heat from the chip assembly 52 and the chamber.

[0131] This embodiment only shows one operation mode of the single-cell nucleic acid processing instrument, and the specific operation mode of the single-cell nucleic acid processing instrument will also have different processes due to different application scenarios, and will be set according to actual needs.

[0132] Note that the above are only preferred embodiments of the present invention and the technical principles employed. Those skilled in the art will understand that the present invention is not limited to the specific embodiments described herein, and that various obvious changes, readjustments, and substitutions can be made by those skilled in the art without departing from the scope of protection of the present invention. Therefore, although the present invention has been described in detail through the above embodiments, the present invention is not limited to the above embodiments and may include many other equivalent embodiments without departing from the concept of the present invention. The scope of the present invention is determined by the scope of the appended claims.

Claims

1. A single-cell nucleic acid processing instrument, characterized in that: include: Rack (11); An air pump assembly is arranged on the frame (11); A box structure, comprising a box drive assembly (31) and a box assembly (32), wherein the box drive assembly (31) is arranged on the frame (11), and the box assembly (32) is arranged at the output end of the box drive assembly (31), and the box drive assembly (31) can drive the box assembly (32) to move in a horizontal direction, and a placement slot (30) is provided on the box assembly (32); A box cover structure, comprising a box cover drive assembly (41) and a box cover assembly (42), wherein the box cover drive assembly (41) is arranged on the frame (11), and the box cover assembly (42) is arranged at the output end of the box cover drive assembly (41) and is located above the box body assembly (32), and the box cover drive assembly (41) can drive the box cover assembly (42) to move in a vertical direction so that the box cover assembly (42) is covered on the box body assembly (32), and the box cover assembly (42) comprises a box cover body (421), and the box cover body (421) is provided with an air inlet hole group and an air extraction hole group, and the air inlet hole group and the air extraction hole group are both connected to the air pump assembly; A chip structure (5) is arranged in the placement groove (30), and the chip structure (5) is provided with a sample addition groove (501), a sample groove (502), a waste liquid groove (503) and a microchannel, the sample addition groove (501) is connected to the air inlet hole group, and the sample groove (502) and the waste liquid groove (503) are both connected to the air extraction hole group; The box cover drive assembly (41) includes two box cover motor assemblies, which are respectively located on the left and right sides of the box cover body (421). Each box cover motor assembly includes: A box cover motor body (411) is arranged on the frame (11); A box cover ball screw, comprising a box cover screw and a box cover nut, wherein the box cover screw is arranged at the output end of the box cover motor body (411), the box cover nut is threadedly connected to the box cover screw, and the box cover nut is fixedly connected to the box cover body (421); The box drive assembly (31) includes: A first box motor (311) is arranged on the frame (11); The box body ball screw (312) comprises a box body screw and a box body nut, wherein the box body screw is arranged at the output end of the first box body motor (311), the box body nut is threadedly connected to the box body screw and is fixedly connected to the box body assembly (32), and the first box body motor (311) can drive the box body screw to rotate so that the box body nut drives the box body assembly (32) to move along the horizontal direction.

2. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The box cover assembly (42) includes a first air intake control valve (422), the air intake hole group includes at least two air intake holes (42102), each of the air intake holes (42102) is provided with a first air intake control valve (422), and the first air intake control valve (422) is configured to control the connection or disconnection between the air intake hole (42102) and the air pump assembly. The box cover body (421) is also provided with an air intake channel (42101), one end of the air intake channel (42101) is respectively connected to the at least two air intake holes (42102), and the other end is connected to the air pump assembly; The box cover assembly (42) includes a first air extraction control valve (423) and a second air extraction control valve (424), the air extraction hole group includes two air extraction holes, the two air extraction holes are respectively a first air extraction hole (421041) and a second air extraction hole (421042), the first air extraction hole (421041) is connected to the waste liquid tank (503) and is provided with the first air extraction control valve (423), the second air extraction hole (421042) is connected to the sample tank (502) and is provided with the second air extraction control valve (424), the first air extraction control valve (423) and the second air extraction control valve (424) are configured to control the connection or disconnection of the air extraction holes and the air pump assembly, and the box cover body (421) is also provided with an air extraction channel (42103), one end of the air extraction channel (42103) is connected to the two air extraction holes, and the other end of the air extraction channel (42103) is connected to the air pump assembly.

3. The single-cell nucleic acid processing instrument according to claim 2, characterized in that: The single-cell nucleic acid processing instrument further comprises an air path integrated block (61), wherein the air path integrated block (61) is provided with a first connecting channel (6101), a second connecting channel (6102) and a first detection channel (6103), one end of the first connecting channel (6101) is connected to the air pump assembly, the other end of the first connecting channel (6101) is connected to the same end of the second connecting channel (6102) and the first detection channel (6103), the other end of the second connecting channel (6102) is connected to the air inlet channel (42101), and the other end of the first detection channel (6103) can be connected to a pressure detector; The gas path integrated block (61) is further provided with a third connecting channel (6104), a fourth connecting channel (6105) and a second detection channel (6106), one end of the third connecting channel (6104) is connected to the air extraction channel (42103), the other end of the third connecting channel (6104) is connected to the same end of the fourth connecting channel (6105) and the second detection channel (6106), the other end of the fourth connecting channel (6105) is connected to the air pump assembly, and the other end of the second detection channel (6106) can be connected to a pressure detector.

4. The single-cell nucleic acid processing instrument according to claim 3, characterized in that: The other end of the first detection channel (6103) is interference fitted with a first plug, and the other end of the second detection channel (6106) is interference fitted with a second plug.

5. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The air pump assembly comprises: An air pump motor is arranged on the frame (11); An air pump ball screw comprises an air pump screw and an air pump nut, wherein the air pump screw is arranged at an output end of the air pump motor, and the air pump nut is threadedly connected to the air pump screw; The air pump body (21) comprises an air pump barrel (211) and an air pump push rod assembly (212), wherein the air pump barrel (211) is arranged on the frame (11) and defines an air pump cavity therein, wherein the air pump cavity can be communicated with the air inlet hole group and the air extraction hole group respectively, one end of the air pump push rod assembly (212) is sealingly and slidingly connected to the air pump barrel (211), and the other end of the air pump push rod assembly (212) is fixedly connected to the air pump nut.

6. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The frame (11) includes a placement plate (12), the cover motor body (411) is arranged on the lower side of the placement plate (12), and the cover drive assembly (41) further includes two cover conveying assemblies (412), the cover conveying assemblies (412) are arranged on the upper side of the placement plate (12), and each of the cover conveying assemblies (412) includes: A first box cover gear (4121) is fixedly arranged at the output end of the box cover motor body (411); A second box cover gear (4122) is fixedly connected to the box cover screw; The box cover conveyor belt (4123) is engaged with the first box cover gear (4121) and the second box cover gear (4122).

7. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The box drive assembly (31) includes: A second box body motor is arranged on the frame (11); The box body gear transmission assembly comprises a meshing box body gear and a box body rack, wherein the box body gear is fixedly arranged at the output end of the second box body motor, the box body rack is slidably arranged on the frame (11) and is fixedly connected to the box body assembly (32), and the second box body motor can drive the box body gear to rotate so that the box body rack drives the box body assembly (32) to move along the horizontal direction.

8. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The box assembly (32) comprises a box body (321), a heat preservation frame (322) and a heat conducting assembly (323); the heat conducting assembly (323) and the box body (321) enclose a chamber with an open top; the heat conducting assembly (323) comprises a heat conducting element (3231), a Peltier and a heat dissipating element (3232) stacked in sequence; the Peltier is located below the heat conducting element (3231) and is capable of heating and cooling the chamber; The heat sink (3232) is located below the Peltier, the heat preservation frame (322) is arranged along the circumference of the heat conducting member (3231), and the heat preservation frame (322) is clamped between the heat conducting member (3231) and the heat sink (3232), and an avoidance hole (3220) is provided on the heat preservation frame (322), and the avoidance hole (3220) is arranged opposite to the heat conducting member (3231) and the heat sink (3232).

9. The single-cell nucleic acid processing instrument according to claim 8, characterized in that: The heat sink (3232) is a heat sink fin or a heat sink plate. A cooling channel is provided in the heat sink plate, and the cooling water in the cooling channel can cool the heat sink plate.

10. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The single-cell nucleic acid processing instrument further includes a magnet feeding structure, the magnet feeding structure including a magnet driving component (71), a connecting component (72) and a magnet arm (73), the magnet driving component (71) is arranged on the frame (11), the connecting component (72) is arranged at the output end of the magnet driving component (71), the connecting component (72) includes a first connecting block (721), a second connecting block (722) and a feeding elastic member (723), the second connecting block (722) is slidably arranged on the first connecting block (721), the feeding elastic member (723) is clamped between the first connecting block (721) and the second connecting block (722). 22), the first connecting block (721) can absorb the second connecting block (722) when it is powered on, and the feed elastic member (723) can reset the second connecting block (722) when it is powered off, the magnet arm (73) is fixedly arranged on the second connecting block (722), the magnet driving component (71) can drive the connecting component (72) to drive the magnet arm (73) to move in the horizontal direction so that the magnet arm (73) is facing the chip structure (5), and when the first connecting block (721) is powered on, the magnet arm (73) can move in the vertical direction toward the chip structure (5) along with the second connecting block (722).

11. The single-cell nucleic acid processing instrument according to claim 1, characterized in that: The chip structure (5) comprises a chip box (51) and at least one chip component (52), wherein the at least one chip component (52) is arranged in the chip box (51), and the chip box (51) is clamped in the placement groove (30).

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