Liquid-based cell preparation device

Abstract

The present invention discloses a liquid-based cell preparation device, which relates to the technical field of cell preparation. The device comprises a bottom plate, and a loading assembly, a circulating transmission assembly, a preparation assembly and a discharging assembly arranged on the bottom plate, wherein the circulating transmission assembly comprises a turntable, and a plurality of positioning seat assemblies arranged in a circular array on the turntable, the loading assembly is used to load a glass slide and a sedimentation bin on the positioning seat assembly, the preparation assembly is used to prepare the glass slide, and the discharging assembly is used to take out and collect the sedimentation bin and the prepared glass slide. The device can avoid the subjectivity of manual sampling and improve the efficiency and accuracy of preparation.

Description

Technical Field

[0001] The present invention relates to the technical field of cell preparation, in particular to a liquid-based cell preparation device. Background Art

[0002] Exfoliative cytology is a diagnostic method that uses normal exfoliated cells from human tissue. Liquid-based cytology is a part of exfoliative cytology. It involves placing exfoliated cytology specimens that are difficult to handle by traditional methods into an intermediate liquid to remove interfering components such as blood and mucus that may affect the diagnosis, thereby increasing the diagnostic rate.

[0003] Liquid-based cytology is mainly used to stain human cell samples with cytological stains to produce standardized cytological smears, which are convenient for diagnosis and meet the requirements of clinical screening and family planning surveys. It is of great significance for medical testing and biological research, especially in cytology and bacterial microbiological testing (gynecological tumor screening, tuberculosis screening, pleural and abdominal effusion tumor cell examination, etc.). However, the manual sample addition, staining, color fixation and slide preparation process is cumbersome and complex, time-consuming and labor-intensive, and difficult to stain and prepare in large quantities and with high efficiency. Summary of the invention

[0004] The object of the present invention is to provide a liquid-based cell preparation device, which can avoid the subjectivity of manual sampling and adding samples, and improve the efficiency and accuracy of preparation.

[0005] The embodiment of the present invention is achieved as follows:

[0006] An embodiment of the present application provides a liquid-based cell preparation device, including a base plate, and a loading component, a circulating transmission component, a preparation component and a discharging component arranged on the base plate, wherein the circulating transmission component includes a turntable, and a plurality of positioning seat components in a circular array on the turntable, the loading component is used to load a glass slide and a sedimentation bin on the positioning seat assembly, the preparation component is used to prepare the glass slide, and the discharging component is used to take out and collect the sedimentation bin and the prepared glass slide.

[0007] Optionally, the loading component includes a slide loading component, which includes a mounting seat, on which a silo is provided, and the silo is used to store slides; the slide loading component also includes a first pushing component that cooperates with the silo transmission, and a second pushing component that cooperates with the mounting seat; the first pushing component includes a first motor and a first gear driven by the first motor, the first pushing component also includes a first slide rail and a first slider slidably connected to the first slide rail, the first slider is respectively provided with a first rack and a first push claw, the first rack is meshed with the first gear, and the first push claw cooperates with the material bin to push the slide in the material bin toward the mounting seat; the second pushing component includes a second motor and a second gear driven by the second motor, the second pushing component also includes a second slide rail and a second slider slidably connected to the second slide rail, the second slider is respectively provided with a second rack and a second push claw, the second rack is meshed with the second gear, and the second push claw cooperates with the mounting seat to push the slide on the mounting seat toward the positioning seat assembly.

[0008] Optionally, the positioning seat assembly includes a positioning seat, and a pressing slider slidably connected to the positioning seat, an elastic member is arranged between the pressing slider and the positioning seat, a first sliding groove is arranged on the pressing slider to allow the slide to slide in, a limiting block is also arranged on the positioning seat, a second sliding groove is arranged on the limiting block, and a corresponding snap-in protrusion is arranged on the sedimentation bin, and the snap-in protrusion can slide into the second sliding groove.

[0009] Optionally, a pressing assembly is arranged above the positioning seat assembly, and the pressing assembly includes a connecting plate, and a third motor and a third slide rail arranged on the connecting plate, the third motor is drivingly connected to the first lead screw, a transmission nut is arranged on the first lead screw, a third slide rail is provided with a third slider slidingly connected to the third slide rail, the third slider is connected to the transmission nut, and a pressure claw is also arranged on the transmission nut or the third slider, and the pressure claw can push the pressing slider to make the pressing slider slide along the positioning seat.

[0010] Optionally, the loading assembly also includes a sedimentation bin loading assembly, which includes at least one barrel, and a translation assembly and a discharge assembly that cooperate with the barrel; the barrel is used to stack sedimentation bins, and the bottom of the barrel has a notch to expose the sedimentation bins; the translation assembly includes a first motor and a first connecting gear driven by the first motor, the translation assembly also includes a first linear slide and a fixed seat slidably connected to the first linear slide, the barrel is arranged on the fixed seat, and the fixed seat is also provided with a first connecting rack meshing with the first connecting gear; the discharge assembly includes a second motor and a second connecting gear driven by the second motor, the discharge assembly also includes a second linear slide and a first sliding block slidably connected to the second linear slide, the first sliding block is provided with a push rod and a second connecting rack, the second connecting rack meshes with the second connecting gear, the push rod corresponds to the barrel, and is used to push the sedimentation bin in the barrel toward the positioning seat assembly.

[0011] Optionally, the circulating transmission assembly further includes a driving member, a rotating seat is correspondingly arranged on the bottom plate, the turntable is arranged on the rotating seat, and the driving member is drivingly connected to the rotating seat so that the rotating seat drives the turntable to rotate.

[0012] Optionally, the film making component includes a sample liquid adding component, the sample liquid adding component includes a first mounting plate and a second mounting plate arranged at intervals, a first sample rack conveying component is arranged on the first mounting plate, and is used to translate the sample rack placed on the first mounting plate, the sample rack is used to place a sample bottle containing the sample liquid, the sample liquid adding component also includes a sampling component, a sample injection component connected to the sampling component is arranged on the bottom plate, and is used to inject the extracted sample liquid into the sedimentation chamber; the sample liquid adding component also includes a second sample rack conveying component, and is used to convey the sample rack placed on the first mounting plate to the second mounting plate.

[0013] Optionally, the first sample rack conveying assembly includes a first rotating motor, a first synchronous wheel is provided at the output end of the first rotating motor, and the first synchronous wheel is connected to the first idle wheel through a first synchronous belt; the first sample rack conveying assembly also includes a first linear guide, a first connecting member is slidably provided on the first linear guide, the first connecting member is fixedly connected to the first synchronous belt, and a pushing member is provided on the first connecting member for pushing the sample rack to move along the extension direction of the first linear guide; the second sample rack conveying assembly includes a first supporting frame, and a second rotating motor arranged on the first supporting frame, the second rotating motor is drivingly connected to the second lead screw, a sliding frame is provided on the second lead screw, a second linear guide is also provided on the first supporting frame, the second linear guide is slidably connected to the sliding frame, a bracket assembly is provided on the sliding frame, the bracket assembly includes a third rotating motor arranged on the sliding frame, the third rotating motor is drivingly connected to the third lead screw, a bracket is provided on the third lead screw, a third linear guide is also provided on the sliding frame, the third linear guide is slidably connected to the bracket, and the bracket is used to hold up the sample rack.

[0014] Optionally, a first guide rail is provided on the second mounting plate, a guide slider is slidably provided on the first guide rail, a slide table is provided on the guide slider, so that the sample rack can slide on the slide table, and a baffle is also provided on the slide table for limiting the sample rack.

[0015] Optionally, the sampling component includes a second support frame, and a first drive motor arranged on the second support frame, the first drive motor is connected to the first screw rod, a second sliding block is slidably arranged on the second support frame, the first screw rod is connected to the first fixed plate, and the first fixed plate is connected to the second sliding block, a second drive motor is arranged on the first fixed plate, the second drive motor is connected to the second screw rod, a third sliding block is arranged on the second screw rod, a sampling needle is arranged on the third sliding block, and an information collection component is also arranged on the third sliding block, and the information collection component is used to collect information on the sample bottle; the sampling component includes a third support frame A rack, and a third driving motor arranged on the third supporting rack, the third driving motor is drivingly connected to the third screw rod, the third screw rod is provided with a fourth sliding block, the fourth sliding block is slidingly connected to the third supporting rack, a second fixed plate is provided on the fourth sliding block, the second fixed plate is provided with a fourth driving motor, the fourth driving motor is drivingly connected to the fourth screw rod, a fifth sliding block is provided on the fourth screw rod, an injection needle is provided on the fifth sliding block, and the sampling needle and the injection needle are connected through a pump body; the sampling component includes a first cleaning tank for cleaning the sampling needle, and the injection component includes a second cleaning tank for cleaning the injection needle.

[0016] Optionally, the film making component also includes a reagent liquid adding component, which includes a plurality of liquid supply bottles and liquid supply pipelines respectively connected to the liquid supply bottles, and a liquid supply pump is provided on the liquid supply pipeline for pumping the reagent liquid in the liquid supply bottle into the sedimentation bin through the liquid supply pipeline, wherein the reagent liquid includes a separation liquid, a buffer solution, a staining liquid, a flushing liquid and a transparent liquid.

[0017] Optionally, the film-making component also includes a waste liquid extraction component, which includes a liquid extraction pump, a liquid extraction pipeline and a waste liquid bottle. The liquid extraction pump is arranged on the liquid extraction pipeline and is used to extract the waste liquid into the waste liquid bottle through the liquid extraction pipeline; the waste liquid extraction component also includes a third cleaning tank for cleaning the liquid extraction pipeline.

[0018] Optionally, the discharge assembly includes a sedimentation bin removal assembly, the sedimentation bin removal assembly includes a first drive motor, a second synchronous wheel is provided at the output end of the first drive motor, and the second synchronous wheel is connected to the second idle wheel through a second synchronous belt; the sedimentation bin removal assembly also includes a second guide rail, a second connecting member is slidably provided on the second guide rail, the second connecting member is fixedly connected to the second synchronous belt, and a hook is provided on the second connecting member; the sedimentation bin removal assembly also includes a guide groove, which is used to make the sedimentation bin on the positioning seat assembly slide out along the guide groove.

[0019] Optionally, the material discharging assembly also includes a glass slide removal assembly, which includes a material removal assembly and a material box cooperating with the material removal assembly, wherein a plurality of protrusions are arranged at intervals in the material box so that the glass slides can be placed at intervals in the material box; the material removal assembly includes a first bracket, and a second drive motor arranged on the first bracket, the second drive motor is drive-connected to a screw rod, a second bracket is arranged on the screw rod, and the second bracket is slidably connected to the first bracket; a third drive motor is arranged on the second bracket, a third synchronous wheel is arranged at the output end of the third drive motor, and the third synchronous wheel is transmission-connected to a third idle wheel through a third synchronous belt; the glass slide removal assembly also includes a third guide rail, a third connecting member is slidably arranged on the third guide rail, the third connecting member is fixedly connected to the third synchronous belt, a manipulator is arranged on the third connecting member, and the manipulator is used to grab the glass slide.

[0020] Optionally, the liquid-based cell preparation device further comprises a coding component, which is located above the positioning seat component and is used for coding the glass slide, and the coding component is communicatively connected with the information acquisition component.

[0021] The beneficial effects of the embodiments of the present invention include:

[0022] The liquid-based cell preparation device provided by the embodiment of the present invention automates the preparation process by means of a loading component, a circulating transmission component, a preparation component and a discharging component arranged on a bottom plate, without the need for manual participation in the preparation. The loading component is used to facilitate the loading of the required glass slide and sedimentation bin on the positioning seat component. Under the action of the circulating transmission component, while driving the positioning seat component to move, the glass slide and the sedimentation bin move synchronously, so as to facilitate the orderly progress of the preparation process. The preparation component arranged on the bottom plate can be used to inject the required sample liquid, extract waste liquid such as supernatant, and automatically add reagents such as staining liquid, thereby completing the preparation process of the glass slide. After the preparation is completed, the sedimentation bin can be taken out by the discharging component, and the prepared glass slide can be taken out and collected for subsequent use. The above-mentioned form can avoid the subjectivity of manual sampling and adding, and improve the efficiency and accuracy of the preparation. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without creative work.

[0024] Figure 1 A schematic diagram of the structure of a liquid-based cell preparation device provided in an embodiment of the present invention;

[0025] Figure 2One of the structural schematic diagrams of the slide loading assembly provided in an embodiment of the present invention;

[0026] Figure 3 A second structural schematic diagram of a slide loading assembly provided in an embodiment of the present invention;

[0027] Figure 4 A schematic diagram of the structure of a positioning seat assembly provided in an embodiment of the present invention;

[0028] Figure 5 A schematic diagram of the structure of a pressing assembly provided in an embodiment of the present invention;

[0029] Figure 6 One of the structural schematic diagrams of the sedimentation bin loading assembly provided in an embodiment of the present invention;

[0030] Figure 7 A second structural schematic diagram of a sedimentation bin loading assembly provided in an embodiment of the present invention;

[0031] Figure 8 A schematic diagram of the structure of a circulating transmission assembly provided in an embodiment of the present invention;

[0032] Fig. 9 A schematic diagram of the structure of a sample liquid adding component provided in an embodiment of the present invention;

[0033] Fig.10 A schematic structural diagram of a first sample rack transport assembly provided in an embodiment of the present invention;

[0034] Fig.11 One of the structural schematic diagrams of the second sample rack transport assembly provided in an embodiment of the present invention;

[0035] Fig.12 A second structural schematic diagram of a second sample rack transport assembly provided by an embodiment of the present invention;

[0036] Fig.13 A schematic diagram of the structure of the cooperation between the second mounting plate and the slide provided in an embodiment of the present invention;

[0037] Fig.14 A schematic diagram of the structure of a sampling component provided in an embodiment of the present invention;

[0038] Fig.15 A schematic diagram of the structure of a sample injection assembly provided in an embodiment of the present invention;

[0039] Fig.16 A schematic diagram of the structure of a sedimentation bin removal assembly provided in an embodiment of the present invention;

[0040] Fig.17 A schematic diagram of the structure of a slide removal assembly provided in an embodiment of the present invention.

[0041] Icons: 10-bottom plate; 103-glass slide; 105-sedimentation bin; 1052-clamping protrusion; 20-loading assembly; 21-glass slide loading assembly; 211-mounting seat; 212-bin; 213-first pushing assembly; 2131-first motor; 2132-first gear; 2133-first slide rail; 2134-first slider; 2135-first rack; 2136-first push claw; 214-second pushing assembly; 2141-second motor; 2142-second gear; 2143-second slide rail; 2144-second slider; 2145-second rack; 2146-second push claw; 23-sedimentation bin loading assembly; 231-barrel; 232-translation assembly; 2321-first motor ;2322-first connecting gear;2323-first linear slide;2324-fixed seat;2325-first connecting rack;234-discharging assembly;2341-second motor;2342-second connecting gear;2343-second linear slide;2344-first sliding block;2345-push rod;2346-second connecting rack;30-circular transmission assembly;31-turntable;32-positioning seat assembly;321-positioning seat;322-pressing slider;3222-first slide;323-elastic member;324-limiting block;3242-second slide;33-driving member;34-rotating seat;40-film making assembly;41-sample liquid adding assembly;412-sampling assembly;4120-first cleaning assembly Washing tank; 4121-second support frame; 4122-first drive motor; 4123-first screw rod; 4124-second sliding block; 4125-first fixed plate; 4126-second drive motor; 4127-second screw rod; 4128-third sliding block; 4129-sampling needle; 414-injection assembly; 4140-second washing tank; 4141-third support frame; 4142-third drive motor; 4143-third screw rod; 4144-fourth sliding block; 4145-second fixed plate; 4146-fourth drive motor; 4147-fourth screw rod; 4148-fifth sliding block; 4149-injection needle; 42-first mounting plate; 43-second mounting plate; 431-first guide rail; 43 2-guide slider; 434-slide; 435-baffle; 44-first sample rack conveying assembly; 441-first rotating motor; 442-first synchronous wheel; 443-first synchronous belt; 444-first idler wheel; 445-first linear guide; 446-first connecting member; 447-pushing member; 45-sample rack; 451-sample bottle; 46-second sample rack conveying assembly; 461-first supporting frame; 462-second rotating motor; 463-second lead screw; 464-sliding frame; 465-second linear guide; 467-bracket assembly; 4671-third rotating motor; 4672-third lead screw; 4673-bracket; 4674-third linear guide; 47-information acquisition assembly; 50-discharging assembly;51-settling bin taking out assembly; 511-first drive motor; 512-second synchronous wheel; 513-second synchronous belt; 514-second idler wheel; 515-second guide rail; 516-second connecting piece; 517-hook; 518-guide groove; 53-slide taking out assembly; 531-material taking out assembly; 5312-first bracket; 5313-second drive motor; 5314-screw; 5315-second bracket; 5316-third drive motor; 5317-third synchronous wheel; 5318-third synchronous belt; 5319-third idler wheel; 533-material box; 534-third guide rail; 535-third connecting piece; 536-manipulator; 60-pressing assembly; 61-connecting plate; 62-third motor; 63-third slide rail; 64-first lead screw; 65-transmission nut; 66-third slider; 67-pressing claw. ; DETAILED DESCRIPTION

[0042] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0043] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0044] It should be noted that similar reference numerals and letters represent similar items in the following drawings, so once an item is defined in one drawing, it does not need to be further defined and explained in the subsequent drawings. In addition, the terms "first", "second", "third", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0045] In the description of the present invention, it is also necessary to explain that, unless otherwise clearly specified and limited, the terms "disposed" and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] Please refer to Figure 1The present embodiment provides a liquid-based cell preparation device, including a base plate 10, and a loading component 20, a circulating transmission component 30, a preparation component 40 and a discharging component 50 arranged on the base plate 10, wherein the circulating transmission component 30 includes a turntable 31, and a plurality of positioning seat components 32 arranged in a circular array on the turntable 31, the loading component 20 is used to load the slide 103 and the sedimentation bin 105 on the positioning seat component 32, the preparation component 40 is used to prepare the slide 103, and the discharging component 50 is used to take out and collect the sedimentation bin 105 and the prepared slide 103.

[0047] Specifically, the loading assembly 20, the film-making assembly 40 and the discharging assembly 50 are distributed around the circulating transmission assembly 30, and cooperate with the circulating transmission assembly 30 to complete the steps of automatic loading, film-making and automatic discharging. Through the turntable 31 of the circulating transmission assembly 30, during the forward or reverse rotation of the turntable 31, the positioning seat assembly 32 on the turntable 31 corresponds to the loading assembly 20, the film-making assembly 40 and the discharging assembly 50 respectively to complete the corresponding operations.

[0048] The liquid-based cell preparation device provided by the embodiment of the present invention, through the loading component 20, the circulating transmission component 30, the preparation component 40 and the discharging component 50 arranged on the bottom plate 10, makes the preparation process automatic, and does not require manual participation in the preparation. The loading component 20 is used to facilitate the required slide 103 and the sedimentation bin 105 to be loaded on the positioning seat component 32. Under the action of the circulating transmission component 30, while driving the positioning seat component 32 to move, the slide 103 and the sedimentation bin 105 are synchronized to facilitate the orderly progress of the preparation process. Through the preparation component 40 arranged on the bottom plate 10, the required sample liquid injection, the extraction of waste liquid such as supernatant, and the automatic addition of reagents such as staining liquid can be performed, thereby completing the preparation process of the slide 103. After the preparation is completed, the sedimentation bin 105 can be taken out through the discharging component 50, and the prepared slide 103 can be taken out and collected for subsequent use. The above method can avoid the subjectivity of manual sampling and improve the efficiency and accuracy of film production.

[0049] like Figure 2 and Figure 3As shown, the loading assembly 20 includes a glass slide loading assembly 21, which includes a mounting seat 211, on which a material bin 212 is provided, and the material bin 212 is used to store the glass slide 103; the glass slide loading assembly 21 also includes a first pushing assembly 213 that is transmission-coordinated with the material bin 212, and a second pushing assembly 214 that is coordinated with the mounting seat 211; the first pushing assembly 213 includes a first motor 2131 and a first gear 2132 that is drive-connected to the first motor 2131, the first pushing assembly 213 also includes a first slide rail 2133 and a first slider 2134 that is slidably connected to the first slide rail 2133, and the first slider 2134 is respectively provided with a first rack 2135 and a first push claw 2136, The first rack 2135 is meshed with the first gear 2132, and the first push claw 2136 cooperates with the hopper 212 to push the glass slide 103 in the hopper 212 toward the mounting seat 211; the second pushing assembly 214 includes a second motor 2141 and a second gear 2142 driven and connected to the second motor 2141, the second pushing assembly 214 also includes a second slide rail 2143 and a second slider 2144 slidably connected to the second slide rail 2143, and the second slider 2144 is respectively provided with a second rack 2145 and a second push claw 2146, the second rack 2145 is meshed with the second gear 2142, and the second push claw 2146 cooperates with the mounting seat 211 to push the glass slide 103 on the mounting seat 211 toward the positioning seat assembly 32.

[0050] Specifically, the extension direction of the first slide rail 2133 is perpendicular to the extension direction of the second slide rail 2143. In the process of loading the glass slide 103, the bottom glass slide 103 in the bin 212 is first pushed sideways to the mounting seat 211 by the first push claw 2136. Then, the glass slide 103 is pushed toward the positioning seat assembly 32 by the second push claw 2146 to complete the loading process of the glass slide 103.

[0051] like Figure 6 and Figure 7As shown, the loading assembly 20 also includes a sedimentation bin loading assembly 23, and the sedimentation bin 105 loading assembly includes at least one barrel 231, and a translation assembly 232 and a discharge assembly 234 that cooperate with the barrel 231; the barrel 231 is used to stack the sedimentation bins 105, and the bottom of the barrel 231 has a notch to expose the sedimentation bins 105; the translation assembly 232 includes a first motor 2321 and a first connecting gear 2322 that is driven and connected to the first motor 2321, the translation assembly 232 also includes a first linear slide rail 2323 and a fixed seat 2324 that is slidably connected to the first linear slide rail 2323, the barrel 231 is arranged on the fixed seat 2324, and the fixed seat 2324 is also provided with a first connecting rack 2325 meshing with the first connecting gear 2322; the discharge assembly 234 includes a second motor 2341 and a second connecting gear 2342 drivingly connected to the second motor 2341, the discharge assembly 234 also includes a second linear slide rail 2343 and a first sliding block 2344 slidingly connected to the second linear slide rail 2343, the first sliding block 2344 is provided with a push rod 2345 and a second connecting rack 2346, the second connecting rack 2346 is meshed with the second connecting gear 2342, the push rod 2345 corresponds to the barrel 231, and is used to push the sedimentation bin 105 in the barrel 231 toward the positioning seat assembly 32.

[0052] Specifically, when the sedimentation bin 105 is loaded into the positioning seat assembly 32, the push rod 2345 is driven by the second motor 2341 to push the sedimentation bin 105 out of the barrel 231. When there are multiple barrels 231, when the sedimentation bin 105 in one of the barrels 231 is completely consumed, in order to ensure the continuous loading of the sedimentation bin 105, the barrel 231 can be driven by the first motor 2321 to translate, so that another barrel 231 with the sedimentation bin 105 corresponds to the push rod 2345, so as to facilitate the continuous loading of the sedimentation bin 105.

[0053] like Figure 4 As shown, the positioning seat assembly 32 includes a positioning seat 321, and a pressing slider 322 slidably connected to the positioning seat, an elastic member 323 is arranged between the pressing slider 322 and the positioning seat, a first slide groove 3222 is arranged on the pressing slider 322 to allow the slide glass 103 to slide in, a limiting block 324 is also arranged on the positioning seat 321, a second slide groove 3242 is arranged on the limiting block 324, and a snap-in protrusion 1052 is correspondingly arranged on the sedimentation bin 105, and the snap-in protrusion 1052 can slide into the second slide groove 3242.

[0054] Specifically, the initial position of the first slide groove 3222 corresponds to the slideway of the slide loading assembly 21 pushing the slide 103, so that the slide 103 can slide into the first slide groove 3222. Since contact sealing is required between the sedimentation bin 105 and the slide 103, the sedimentation bin 105 and the slide 103 are abutted against each other under the action of the elastic member 323. Therefore, when the sedimentation bin 105 slides into the second slide groove 3242, the pressing slider 322 needs to be pressed down to avoid interference between the sedimentation bin 105 and the slide 103 when the sedimentation bin 105 slides into the second slide groove 3242. After the sedimentation bin 105 is in place, the pressing of the pressing slider 322 is released, and the required contact sealing is achieved when the elastic member 323 recovers its elastic deformation.

[0055] like Figure 5 As shown, a pressing assembly 60 is arranged above the positioning seat assembly 32, and the pressing assembly 60 includes a connecting plate 61, and a third motor 62 and a third slide rail 63 arranged on the connecting plate 61, the third motor 62 is drivingly connected to the first lead screw 64, a transmission nut 65 is arranged on the first lead screw 64, and a third slide rail 63 is provided with a third slider 66 slidably connected to the third slide rail 63, the third slider 66 is connected to the transmission nut 65, and a pressing claw 67 is also arranged on the transmission nut 65 or the third slider 66, and the pressing claw 67 can push the pressing slider 322 to make the pressing slider 322 slide along the positioning seat 321.

[0056] Specifically, when loading the sedimentation bin 105, the pressing claw 67 is controlled by the third motor 62 to press down, so that the pressing slider 322 slides along the positioning seat 321. In this way, the second slide groove 3242 can correspond to the sedimentation bin 105 loading component, which is convenient for loading the sedimentation bin 105 and can also avoid interference between the sedimentation bin 105 and the slide glass 103, which is conducive to the smooth loading. It should be noted that a pressing component 60 is provided at the sedimentation bin 105 loading position and the sedimentation bin 105 collection position. When taking out the sedimentation bin 105, the pressing slider 322 needs to be pressed down to separate the sedimentation bin 105 from the slide glass 103, ensuring that the sedimentation bin 105 will not affect the slide glass 103 after the preparation is completed when it is taken out.

[0057] like Figure 8 As shown, the circulating transmission assembly 30 also includes a driving member 33. A rotating seat 34 is correspondingly arranged on the bottom plate 10. The turntable 31 is arranged on the rotating seat 34. The driving member 33 is drivingly connected to the rotating seat 34 so that the rotating seat 34 drives the turntable 31 to rotate.

[0058] Specifically, the driving member 33 may be a servo motor to precisely control the turntable 31. The driving member 33 and the rotating seat 34 may be connected by a belt. The present application does not impose any specific restrictions on this, as long as the required transmission relationship can be met. When the turntable 31 rotates, the positioning seat assemblies 32 at different positions on the turntable 31 are driven in a circular cycle to achieve the required film making process.

[0059] like Figures 9 to 12 As shown, the film making component 40 includes a sample liquid adding component 41, and the sample liquid adding component 41 includes a first mounting plate 42 and a second mounting plate 43 which are spaced apart. A first sample rack conveying component 44 is provided on the first mounting plate 42 for translating a sample rack 45 placed on the first mounting plate 42, and a sample bottle 451 containing sample liquid is placed on the sample rack 45. The sample liquid adding component 41 also includes a sampling component 412, and a sample injection component 414 which is connected to the sampling component 412 is provided on the bottom plate 10 for injecting the extracted sample liquid into the sedimentation chamber 105; the sample liquid adding component 41 also includes a second sample rack conveying component 46 for conveying the sample rack 45 placed on the first mounting plate 42 to the second mounting plate 43.

[0060] Specifically, the first sample rack conveying component 44 pushes the sample rack 45 placed on the first mounting plate 42 to the sampling component 412, so that the sampling component 412 can sample the sample bottles 451 on the sample rack 45. After the sampling of the sample bottles 451 on the sample rack 45 at the sampling component 412 is completed, the sample rack 45 is conveyed to the second mounting plate 43 by the second sample rack conveying component 46, so that the next sample rack 45 can be pushed to the sampling component 412 for sampling.

[0061] like Fig.10 As shown, the first sample rack conveying assembly 44 includes a first rotary motor 441, and a first synchronous wheel 442 is provided at the output end of the first rotary motor 441, and the first synchronous wheel 442 is connected to the first idle wheel 444 through a first synchronous belt 443; the first sample rack conveying assembly 44 also includes a first linear guide 445, and a first connecting member 446 is slidably provided on the first linear guide 445, and the first connecting member 446 is fixedly connected to the first synchronous belt 443, and a pushing member 447 is provided on the first connecting member 446, which is used to push the sample rack 45 to move along the extension direction of the first linear guide 445. Thus, the continuous conveying sampling action of the sample rack 45 is realized.

[0062] like Fig.11 and 12As shown, the second sample rack conveying assembly 46 includes a first support frame 461, and a second rotating motor 462 arranged on the first support frame 461, the second rotating motor 462 is drivingly connected to the second lead screw 463, a sliding frame 464 is arranged on the second lead screw 463, a second linear guide 465 is also arranged on the first support frame 461, the second linear guide 465 is slidably connected to the sliding frame 464, a bracket assembly 467 is arranged on the sliding frame 464, the bracket assembly 467 includes a third rotating motor 4671 arranged on the sliding frame 464, the third rotating motor 4671 is drivingly connected to the third lead screw 4672, a bracket 4673 is arranged on the third lead screw 4672, a third linear guide 4674 is also arranged on the sliding frame 464, the third linear guide 4674 is slidably connected to the bracket 4673, and the bracket 4673 is used to hold up the sample rack 45.

[0063] Specifically, after sampling of the sample bottle 451 on the sample rack 45 at the sampling component 412 is completed, the sample rack 45 is lifted by the bracket 4673, so that the sample rack 45 is transferred from the first mounting plate 42 to the second mounting plate 43 and pushed forward one position to facilitate the continuous placement of the sample rack 45.

[0064] like Fig.13 As shown, a first guide rail 431 is provided on the second mounting plate 43, a guide slider 432 is slidably provided on the first guide rail 431, a slide table 434 is provided on the guide slider 432, so that the sample rack 45 can slide on the slide table 434, and a baffle 435 is also provided on the slide table 434 for limiting the sample rack 45.

[0065] Specifically, the sample rack 45 transferred to the second mounting plate 43 by the second sample rack transfer assembly 46 is located on the slide 434 and can slide along the slide 434. When the sample rack 45 placed on the slide 434 reaches a preset condition, the slide 434 can be pulled out to facilitate the sample rack 45 to be taken out for recycling. In order to facilitate the pulling out of the slide 434, a handle can be provided on the baffle 435 so that the slide 434 can be pulled out by the handle.

[0066] like Fig.14As shown, the sampling assembly 412 includes a second support frame 4121, and a first drive motor 4122 disposed on the second support frame 4121, the first drive motor 4122 is connected to the first screw rod 4123, a second sliding block 4124 is slidably disposed on the second support frame 4121, the first screw rod 4123 is connected to the first fixed plate 4125, and the first fixed plate 4125 is connected to the second sliding block 4124, a second drive motor 4126 is disposed on the first fixed plate 4125, the second drive motor 4126 is connected to the second screw rod 4127, a third sliding block 4128 is disposed on the second screw rod 4127, a sampling needle 4129 is disposed on the third sliding block 4128, and an information collection assembly 47 is also disposed on the third sliding block 4128, and the information collection assembly 47 is used to collect information on the sample bottle 451. In this way, when the sample in the sample bottle 451 is extracted, the information of the sample can be recorded, so as to facilitate the recording and archiving of the detection of the sample.

[0067] Please refer to Fig.15 The injection assembly 414 includes a third support frame 4141, and a third drive motor 4142 disposed on the third support frame 4141, the third drive motor 4142 is drivingly connected to a third screw rod 4143, a fourth sliding block 4144 is disposed on the third screw rod 4143, the fourth sliding block 4144 is slidably connected to the third support frame 4141, a second fixing plate 4145 is disposed on the fourth sliding block 4144, and the second fixing plate 4145 is disposed on the fourth drive motor 4142. 146, the fourth driving motor 4146 is drivingly connected to the fourth screw rod 4147, the fourth screw rod 4147 is provided with a fifth sliding block 4148, the fifth sliding block 4148 is provided with a sample injection needle 4149, the sampling needle 4129 is connected to the sample injection needle 4149 through a pump body; the sampling component 412 includes a first cleaning tank 4120 for cleaning the sampling needle 4129, and the sample injection component 414 includes a second cleaning tank 4140 for cleaning the sample injection needle 4149.

[0068] Specifically, when cleaning the sampling needle 4129 and the injection needle 4149, not only the outer wall surface but also the inner surface should be cleaned to avoid affecting the accuracy of the next sample detection. When cleaning the inner wall surface, cleaning can be performed by extracting a cleaning liquid.

[0069] Optionally, the film-making component 40 further includes a reagent liquid adding component, which includes a plurality of liquid supply bottles and liquid supply pipelines respectively connected to the liquid supply bottles, and a liquid supply pump is provided on the liquid supply pipeline to pump the reagent liquid in the liquid supply bottle into the sedimentation bin 105 through the liquid supply pipeline, wherein the reagent liquid includes a separation liquid, a buffer solution, a staining liquid, a flushing liquid and a transparent liquid. Specifically, the reagent liquid adding component can be set to a plurality of components, each of which is responsible for adding a reagent liquid. In this way, there is no need to clean the reagent liquid adding component, which is conducive to ensuring the efficient progress of the film-making process.

[0070] Optionally, the film-making component 40 also includes a waste liquid extraction component, which includes a liquid extraction pump, a liquid extraction pipeline and a waste liquid bottle. The liquid extraction pump is arranged on the liquid extraction pipeline, and is used to extract the waste liquid into the waste liquid bottle through the liquid extraction pipeline; the waste liquid extraction component also includes a third cleaning tank, which is used to clean the liquid extraction pipeline. Specifically, the specific structure of the waste liquid extraction component can adopt the structural form of the injection component 414 to extract liquid, except that the flow direction of the liquid is changed by the liquid extraction pump. Among them, when the waste liquid is extracted, the liquid extraction pipeline also needs to be cleaned to avoid affecting the film-making effect.

[0071] like Fig.16 As shown, the discharge assembly 50 includes a sedimentation bin removal assembly 51, and the sedimentation bin removal assembly 51 includes a first drive motor 511. The output end of the first drive motor 511 is provided with a second synchronous wheel 512, and the second synchronous wheel 512 is transmission-connected to the second idle wheel 514 through a second synchronous belt 513; the sedimentation bin removal assembly 51 also includes a second guide rail 515, and a second connecting member 516 is slidingly provided on the second guide rail 515. The second connecting member 516 is fixedly connected to the second synchronous belt 513, and a hook 517 is provided on the second connecting member 516; the sedimentation bin removal assembly 51 also includes a guide groove 518, which is used to make the sedimentation bin 105 on the positioning seat assembly 32 slide out along the guide groove 518.

[0072] Specifically, in the initial state, the hook 517 is located inside the positioning seat assembly 32 and does not affect the positioning seat assembly 32 to rotate with the turntable 31. When the sedimentation bin 105 needs to be taken out, the sedimentation bin 105 is made to correspond to the hook 517 to hook out the sedimentation bin 105. When hooking out the sedimentation bin 105, the sedimentation bin 105 needs to be separated from the slide.

[0073] like Fig.17As shown, the discharging assembly 50 also includes a glass slide taking-out assembly 53, which includes a material taking-out assembly 531 and a material box 533 cooperating with the material taking-out assembly 531, wherein a plurality of protrusions are arranged at intervals in the material box 533 so that the glass slides 103 are placed at intervals in the material box 533; the material taking-out assembly 531 includes a first bracket 5312 and a second driving motor 5313 arranged on the first bracket 5312, wherein the second driving motor 5313 is drivingly connected to a screw rod 5314, wherein a second bracket 5315 is arranged on the screw rod 5314, and the second bracket 5315 is connected to the first bracket 5312. 312 is slidingly connected; a third driving motor 5316 is arranged on the second bracket 5315, and a third synchronous wheel 5317 is arranged at the output end of the third driving motor 5316, and the third synchronous wheel 5317 is transmission-connected to the third idle wheel 5319 through a third synchronous belt 5318; the glass slide taking-out assembly 53 also includes a third guide rail 534, and a third connecting member 535 is slidingly arranged on the third guide rail 534, and the third connecting member 535 is fixedly connected to the third synchronous belt 5318, and a manipulator 536 is arranged on the third connecting member 535, and the manipulator 536 is used to grab the glass slide 103.

[0074] By adopting the above-mentioned method, through the cooperation between the first driving motor 511 and the second driving motor 5313, the slides 103 that have been prepared can be collected, which is beneficial to improving the collection efficiency and the automation of the collection.

[0075] Optionally, the liquid-based cell preparation device further includes a coding component, which is located above the positioning seat component 32 and is used to code the glass slide 103. The coding component is communicatively connected to the information collection component 47. In this way, the information on the sample bottle 451 corresponds to the information on the glass slide 103, which is conducive to improving the accuracy of the data and facilitating statistical sorting.

[0076] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A liquid-based cell preparation device, characterized in that: The invention comprises a bottom plate, and a loading assembly, a circulating transmission assembly, a film-making assembly and a discharging assembly arranged on the bottom plate, wherein the circulating transmission assembly comprises a turntable, and a plurality of positioning seat assemblies arranged in a circumferential array on the turntable, the loading assembly is used to load a glass slide and a sedimentation bin on the positioning seat assembly, the film-making assembly is used to make films of the glass slide, and the discharging assembly is used to take out and collect the sedimentation bin and the glass slide after making films; The loading assembly includes a slide loading assembly, the slide loading assembly includes a mounting seat, a material bin is provided on the mounting seat, and the material bin is used to store the slides; the slide loading assembly also includes a first pushing assembly that cooperates with the material bin in transmission, and a second pushing assembly that cooperates with the mounting seat; The slide making assembly includes a sample liquid adding assembly, which includes a first mounting plate and a second mounting plate arranged at intervals, a first sample rack conveying assembly is arranged on the first mounting plate, and is used to translate the sample rack placed on the first mounting plate, and the sample rack is used to place a sample bottle containing the sample liquid, the sample liquid adding assembly also includes a sampling assembly, and a sample injection assembly connected to the sampling assembly is arranged on the bottom plate, and is used to inject the extracted sample liquid into the sedimentation chamber; the sample liquid adding assembly also includes a second sample rack conveying assembly, and is used to convey the sample rack placed on the first mounting plate to the second mounting plate; The discharging assembly includes a sedimentation bin taking-out assembly, and the sedimentation bin taking-out assembly includes a first driving motor, and the output end of the first driving motor is provided with a second synchronous wheel, and the second synchronous wheel is connected to the second idle wheel through a second synchronous belt; the sedimentation bin taking-out assembly also includes a second guide rail, and a second connecting piece is slidably provided on the second guide rail, and the second connecting piece is fixedly connected to the second synchronous belt, and a hook claw is provided on the second connecting piece; the sedimentation bin taking-out assembly also includes a guide groove, which is used to make the sedimentation bin on the positioning seat assembly slide out along the guide groove.

2. The liquid-based cell preparation device according to claim 1, characterized in that: The first pushing assembly includes a first motor and a first gear drivingly connected to the first motor, the first pushing assembly also includes a first slide rail and a first slider slidably connected to the first slide rail, the first slider is respectively provided with a first rack and a first push claw, the first rack is meshed with the first gear, and the first push claw cooperates with the bin to push the slide in the bin toward the mounting seat; The second pushing assembly includes a second motor and a second gear drivingly connected to the second motor. The second pushing assembly also includes a second slide rail and a second slider slidably connected to the second slide rail. The second slider is respectively provided with a second rack and a second push claw. The second rack is meshed with the second gear. The second push claw cooperates with the mounting seat to push the slide on the mounting seat toward the positioning seat assembly.

3. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The positioning seat assembly includes a positioning seat and a pressing slider slidably connected to the positioning seat, an elastic member is arranged between the pressing slider and the positioning seat, a first slide groove is arranged on the pressing slider to allow the slide to slide in, a limiting block is also arranged on the positioning seat, a second slide groove is arranged on the limiting block, and a snap-in protrusion is correspondingly arranged on the sedimentation bin, and the snap-in protrusion can slide into the second slide groove.

4. The liquid-based cell preparation device according to claim 3, characterized in that: A pressing assembly is arranged above the positioning seat assembly, and the pressing assembly includes a connecting plate, and a third motor and a third slide rail arranged on the connecting plate, the third motor is drivingly connected to the first lead screw, a transmission nut is arranged on the first lead screw, and a third slide rail is provided with a third slider slidably connected to the third slide rail, the third slider is connected to the transmission nut, and a pressure claw is also arranged on the transmission nut or the third slider, and the pressure claw can push the pressing slider to make the pressing slider slide along the positioning seat.

5. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The loading assembly also includes a sedimentation bin loading assembly, which includes at least one barrel, and a translation assembly and a discharge assembly that cooperate with the barrel; the barrel is used to stack the sedimentation bins, and the bottom of the barrel has a notch to expose the sedimentation bins; the translation assembly includes a first motor and a first connecting gear driven by the first motor, the translation assembly also includes a first linear slide rail and a fixed seat slidably connected to the first linear slide rail, the barrel is arranged on the fixed seat, and the fixed seat is also provided with a first connecting rack meshing with the first connecting gear; the discharge assembly includes a second motor and a second connecting gear driven by the second motor, the discharge assembly also includes a second linear slide rail and a first sliding block slidably connected to the second linear slide rail, the first sliding block is provided with a push rod and a second connecting rack, the second connecting rack meshes with the second connecting gear, and the push rod corresponds to the barrel, and is used to push the sedimentation bin in the barrel toward the positioning seat assembly.

6. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The circulating transmission assembly also includes a driving member. A rotating seat is correspondingly arranged on the bottom plate. The turntable is arranged on the rotating seat. The driving member is drivingly connected to the rotating seat so that the rotating seat drives the turntable to rotate.

7. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The first sample rack conveying assembly includes a first rotary motor, a first synchronous wheel is provided at the output end of the first rotary motor, and the first synchronous wheel is connected to the first idle wheel through a first synchronous belt; the first sample rack conveying assembly also includes a first linear guide, a first connecting member is slidably provided on the first linear guide, the first connecting member is fixedly connected to the first synchronous belt, and a pushing member is provided on the first connecting member for pushing the sample rack to move along the extension direction of the first linear guide; the second sample rack conveying assembly includes a first support frame, and a second rotary motor provided on the first support frame, the second rotary motor is connected to the second lead screw for driving, a sliding frame is provided on the second lead screw, a second linear guide is also provided on the first support frame, the second linear guide is slidably connected to the sliding frame, a bracket assembly is provided on the sliding frame, the bracket assembly includes a third rotary motor provided on the sliding frame, the third rotary motor is connected to the third lead screw for driving, a bracket is provided on the third lead screw, a third linear guide is also provided on the sliding frame, the third linear guide is slidably connected to the bracket, and the bracket is used to hold up the sample rack.

8. The liquid-based cell preparation device according to claim 7, characterized in that: The second mounting plate is provided with a first guide rail, a guide slider is slidably provided on the first guide rail, a slide table is provided on the guide slider, so that the sample rack can slide on the slide table, and a baffle is also provided on the slide table for limiting the sample rack.

9. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The sampling component includes a second support frame, and a first driving motor arranged on the second support frame, the first driving motor is drivingly connected to the first screw rod, a second sliding block is slidably arranged on the second support frame, the first screw rod is connected to the first fixed plate, and the first fixed plate is connected to the second sliding block, a second driving motor is arranged on the first fixed plate, the second driving motor is drivingly connected to the second screw rod, a third sliding block is arranged on the second screw rod, a sampling needle is arranged on the third sliding block, and an information collection component is also arranged on the third sliding block, and the information collection component is used to collect information on the sample bottle; The injection assembly includes a third support frame, and a third drive motor arranged on the third support frame, the third drive motor is drivingly connected to the third screw rod, the third screw rod is provided with a fourth sliding block, the fourth sliding block is slidably connected to the third support frame, the fourth sliding block is provided with a second fixed plate, the second fixed plate is provided with a fourth drive motor, the fourth drive motor is drivingly connected to the fourth screw rod, the fourth screw rod is provided with a fifth sliding block, the fifth sliding block is provided with an injection needle, and the sampling needle is connected to the injection needle through a pump body; The sampling component comprises a first cleaning tank for cleaning the sampling needle, and the injection component comprises a second cleaning tank for cleaning the injection needle.

10. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The film making component also includes a reagent liquid adding component, which includes a plurality of liquid supply bottles and liquid supply pipelines respectively connected to the liquid supply bottles. The liquid supply pipelines are provided with liquid supply pumps for pumping the reagent liquid in the liquid supply bottles into the sedimentation bin through the liquid supply pipelines, wherein the reagent liquid includes separation liquid, buffer solution, staining liquid, flushing liquid and transparent liquid.

11. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The film-making component also includes a waste liquid extraction component, which includes a liquid extraction pump, a liquid extraction pipeline and a waste liquid bottle. The liquid extraction pump is arranged on the liquid extraction pipeline and is used to extract the waste liquid into the waste liquid bottle through the liquid extraction pipeline; the waste liquid extraction component also includes a third cleaning tank for cleaning the liquid extraction pipeline.

12. The liquid-based cell preparation device according to claim 1 or 2, characterized in that: The material discharging assembly also includes a glass slide removal assembly, which includes a material removal assembly and a material box cooperating with the material removal assembly, wherein a plurality of protrusions are arranged at intervals in the material box so that the glass slides can be placed at intervals in the material box; the material removal assembly includes a first bracket, and a second driving motor arranged on the first bracket, the second driving motor is connected to the screw rod for driving, the screw rod is provided with a second bracket, and the second bracket is slidably connected to the first bracket; a third driving motor is arranged on the second bracket, and a third synchronous wheel is arranged at the output end of the third driving motor, and the third synchronous wheel is connected to the third idle wheel through a third synchronous belt; the glass slide removal assembly also includes a third guide rail, a third connecting member is slidably arranged on the third guide rail, the third connecting member is fixedly connected to the third synchronous belt, a manipulator is arranged on the third connecting member, and the manipulator is used to grab the glass slide.

13. The liquid-based cell preparation device according to claim 9, characterized in that: The liquid-based cell preparation device also includes a coding component, which is located above the positioning seat component and is used to code the glass slide. The coding component is communicatively connected with the information collection component.

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