Synthetic chimeric vaccinia virus

Abstract

The present invention relates in various aspects to synthetic chimeric vaccinia viruses or compositions comprising the same, and the development and use of systems and methods for producing the synthetic chimeric vaccinia viruses. The synthetic chimeric vaccinia viruses are particularly suitable as viral vaccines or for producing oncolytic responses and pharmaceutical preparations.

Description

Background Art

[0001] The sequence listing associated with this application is submitted electronically in text format via EFS-Web and is hereby incorporated by reference into the specification in its entirety. The text file containing the sequence listing is named 104545-0031-WO-SequenceListing.txt. The text file was created on May 2, 2019, and is 288,652 bytes in size.

[0002] Poxviruses (members of the Poxviridae family) are double-stranded DNA viruses that can infect both humans and animals. Poxviruses are divided into two subfamilies based on their host range. The Chordopoxviridae subfamily, which infects vertebrate hosts, consists of eight genera, four of which (Orthopoxvirus, Parapoxvirus, Molluscipoxvirus, and Yatapoxvirus) are known to infect humans. Smallpox is caused by infection with variola virus (VARV), a member of the genus Orthopoxvirus (OPV). The OPV genus includes many genetically related and morphologically identical viruses, including camelpox virus (CMLV), cowpox virus (CPXV), ectromelia virus (ECTV, "mousepox agent"), horsepox virus (HPXV), monkeypox virus (MPXV), rabbitpox virus (RPXV), raccoonpox virus, skunkpox virus, taterapox virus, Uasin Gishu disease virus, vaccinia virus (VACV), variolavirus (VARV) and volepox virus (VPV). In addition to VARV, at least three other OPVs (including VACV, MPXV and CPXV) are known to infect humans. To date, vaccination with "live" VACV is the only proven protection against smallpox. An aggressive vaccination program led to the eradication of smallpox in 1980, and routine smallpox vaccination of the general public was discontinued. However, there remains a need to find new safe and effective methods for individual vaccination against VARV and other OPVs.

[0003] Various formulations of VACV have been used as smallpox vaccines. Most of these vaccines are composed of multiple related viruses (e.g., Dryvax), and one contains a single molecular clone, ACAM2000. However, like Dryvax and other VACV vaccines, even ACAM2000 is associated with serious side effects including cardiomyopathy and pericarditis. To reduce the risk, the ACAM2000 vaccine, like other live vaccines, has many contraindications, excluding individuals with cancer, immunodeficiency, organ transplant recipients, patients with atopic dermatitis, eczema, psoriasis, heart disease, and patients taking immunosuppressants. It is estimated that 15-50% of the US population falls into one of these categories, confirming the need for the development of safer vaccines or vaccination regimens (Kennedy et al., 2007 Kennedy R, Poland GA. 2007. T-Cell epitope discovery for variola and vaccinia viruses. Rev Med Virol 7:93-113). Therefore, there is a need to develop a vaccine with efficacy similar to that of Dryvax or ACAM2000. TM But a safer vaccine.

[0004] Production safety, purity, power and effective vaccine need quality assurance program to ensure consistency and the continuity of vaccine production process. In the past, embryonic egg or primary chicken embryo fibroblast culture have been used to cultivate viruses to prepare vaccines against yellow fever, influenza, measles and mumps. These matrices are considered to be acceptable because the exogenous factors that think can infect chicken will not infect humans and not cause disease to humans (FDA briefing document vaccine and related biological products advisory committee meeting (FDA Briefing Document Vaccines and Related Biological Products Advisory Committee Meeting), on September 19, 2012). However, if the virus tropism changes, safety can be compromised.

[0005] Other matrices have been used to culture viruses for vaccine production, such as calf lymph for the smallpox vaccine. After calves are vaccinated against smallpox, lymph containing white blood cells is extracted and stored in capillaries. This is then used for human vaccination against smallpox. However, there is a risk of contamination with bovine spongiform encephalopathy or scrapie prions. Although regulations and guidelines for modern vaccines state that all materials used must come from BSE-free areas, there are no regulations or guidelines regarding the status of scrapie-free areas. Of particular concern is the fact that the Dryvax vaccine produced in 1980–1982 was not scrutinized by modern methods. Specifically, these stock solutions were not tested for adventitious agents (Murphy and Osburn. Emerging Infectious Diseases. www.cdc.gov / eid .Volume 11, No.7, July 2005).

[0006] Therefore, there is a need to develop a vaccine with efficacy similar to that of existing Dryvax or ACAM2000 TM vaccines, but are safer, replicable, and free of residual cells, residual DNA, prions, and exogenous agents.

[0007] The present application provides chimeric vaccinia viruses assembled and replicated from chemically synthesized DNA that are safe, replicable, and free of contaminants. Because chemical genome synthesis does not rely on a natural template, a variety of structural and functional modifications of the viral genome are possible. Chemical genome synthesis is particularly useful when no natural template is available for genetic replication or modification by conventional molecular biology methods. SUMMARY OF THE INVENTION

[0009] One aspect of the present invention provides synthetic chimeric vaccinia viruses, methods for producing such viruses, and uses of the viruses, for example, as immunogens, in immunogenic formulations, in in vitro assays, as vectors for heterologous gene expression, or as oncolytic agents for treating cancer. The synthetic chimeric vaccinia viruses of the present application are characterized by one or more modifications relative to wild-type vaccinia virus.

[0010] In one aspect, the invention is based on the discovery that synthetic chimeric vaccinia viruses (eg, scVACV) can be generated from chemically synthesized overlapping segments of the vaccinia virus genome.

[0011] Thus, in one aspect, the present invention relates to synthetic chimeric vaccinia viruses (e.g., scVACV) that are replicated and reactivated from DNA derived from synthetic DNA, wherein the viral genome of the virus differs from the wild-type genome of the virus by being characterized by one or more modifications derived from a group comprising chemically synthesized DNA, cDNA, or genomic DNA.

[0012] In another aspect, the present invention relates to a method for preparing a synthetic chimeric vaccinia virus (scVACV), comprising the steps of: (i) chemically synthesizing overlapping DNA segments corresponding to substantially the entire viral genome of a vaccinia virus; (ii) transfecting the overlapping DNA segments into helper virus-infected cells; (iii) culturing the cells to produce a mixture of helper virus and synthetic chimeric vaccinia particles in the cells; and (iv) plating the mixture on host cells specific for scVACV to recover the scVACV.

[0013] In another aspect, the present invention relates to a synthetic chimeric vaccinia virus (scVACV) produced by the method of the present invention.

[0014] In another aspect, the present invention relates to a pharmaceutical composition comprising the synthetic chimeric vaccinia virus (scVACV) of the present invention and a pharmaceutically acceptable carrier.

[0015] In another aspect, the present invention relates to a method of inducing an oncolytic response in a subject, comprising administering to the subject a composition comprising a scVACV of the present invention.

[0016] In another aspect, the present invention relates to a method for expressing a heterologous protein in a host cell, comprising introducing a heterologous nucleic acid sequence into the scVACV of the present invention, infecting the host cell with the scVACV, and culturing the host cell under conditions for expressing the heterologous protein.

[0017] In another aspect, the present invention relates to a method of triggering or enhancing an immune response against vaccinia virus, comprising administering to a subject in need thereof a composition comprising a scVACV of the present invention.

[0018] In another aspect, the present invention relates to a method of triggering or enhancing an immune response to smallpox virus infection, comprising administering to the subject a composition comprising a scVACV of the present invention.

[0019] In another aspect, the present invention relates to a method of triggering or enhancing an immune response to monkeypox virus infection, comprising administering to the subject a composition comprising a scVACV of the present invention.

[0020] In another aspect, the invention relates to a method of immunizing a human subject to protect said subject from infection with smallpox virus, comprising administering to said subject a composition comprising a scVACV of the invention.

[0021] In another aspect, the present invention relates to a method of treating a smallpox virus infection comprising administering to the subject a composition comprising a scVACV of the present invention.

[0022] In another aspect, the present invention relates to a method of treating cancer in a subject, comprising administering to a subject in need thereof a composition comprising a scVACV of the present invention. Sequence Listing <110> EVANS, DAVID NOYCE, RYAN LEDERMAN, SETH <120> Synthetic chimeric vaccinia virus <130> 104545-0031-WO1 <140> <141> <150> 62 / 665,973 <151> 2018-05-02 <160> twenty two <170> PatentIn version 3.5 <210> 1 <211> 18525 <212> DNA <213> Vaccinia virus <400> 1 tagggataac agggtaatga agaagctctt cgccagaagc tagcaagagg tttaatattt 60 ttgtgagacc atcgaagaga gaaagagaat aaaaatattt tatgactcca ttgaagagag 120 aaagagaaaa tgagaatgag aataaaaata ttttagtgac accatcagaa agaggtttaa 180 tatttttgtg agaccatcga agagagaaag agaataaaaa tattttatga ctccattgaa 240 gagagaaaga gaaaatgaga atgagaataa aaatatttta gtgacaccat cagaaagagg 300 tttaatattt tttatgagac catcaaagag agaaagagaa taaaaatatt tttgtaaaac 360 tttttttg agaccatca agagagaaag agataaaaa tattttgta aaactttttt 420 tatgagacca tcaagagag aaagaata aaatatttt tgtaaaactt ttttttagag 480 accatcaag aggaagag aaaaaata ttttgtaaa acttttttta tgagaccatc 540 aaagagagaa agagaataa atattttg taaaactttt ttttagagac catchagag 600 agaaagagaaaaatatt ttgtaaaac ttttttg agaccatcha agagagaaag 660 agataaaaa tattttgta aaactttttt tatgagacca tcaagagag aaagaata 720 aaatatttt tgtaaaactt tttttgag accatcagaa agaggttta tattttgtg 780 ataccctgaa aggaatagg ataggaata ggaatagtgt cataatcgta tcacactatt 840 gagacagaaaagaaggt cgcgagaggt aactttttgt gatgtagtt aagaacattt 900 ttgttttgca aaccggaata tagtgtccgg tacacttt taattcgtgg tgtgcctgaa 960 tcgttcgatt aaccctactc atccaatttc agatgaatag agttatcgat tcagacacac 1020 gctttgagtt ttgttgaatc gatgagtgaa gtatcatcgg ttgcaccttc agatgccgat 1080 ccgtcgacat acttgaatcc atccttgacc tcaagttcag atgattcctt gcacatgtct 1140 ccgatacgaa cgctaaactc tagattcttg acgcattttg tatcgacgat cgttgaaccg 1200 atgatatctt cgtaactcac tttcttatga gagatgttag acccgagtac tggatgggtc 1260 ttgatgtcgc tgtctttctc ttcttcgcta catctgatgt cgatagacac ctcacagtct 1320 ttgatcatag caagagcttc ttcatgagtg atcgcgggag agtccttacc ttgtcctggg 1380 gacacgctgg acaatctagc attcactgtg tttccatcag cggattctga gatggattta 1440 atctgaggac atttggtgaa tccaaagttc attctcagac ctccaccgat gatggagtaa 1500 taagtggtag gaggatctac atcctcgact gatgtggaat catcttctga ttccacctcg 1560 ggatctggat ctgactcgga ctctgtaatt tccgttacgg attggcaaat cttatcattg 1620 gtcggtgttt ggtcttgctt tgtgactttg ataataacat cgattcccat atgatgtttg 1680 ttttcttctt ccgtacacga ggaggaggat tgctgaagac tggcaggcac atgcatgcca 1740 ggacgatata ttgtttcatg attgctattg attgattact gttctttatg attctacttc 1800 cttaccgtgc aataaattag aatatatttt ctacttttac gagaaattaa ttatgtatt 1860 tatgggtgaa aaacttacta taaaaagcgg gtgggtttgg aattagtgat cagtttatgt 1920 atatcgcaac taccgggcat atggctatcg acatcgagaa cattacccac atgataagag 1980 attgtatcag tttcgtagtc ttgagtattg gtattactat atagtatata gatgtcgacg 2040 ctatagac agtctccgaa tgcggcatga tagtatcatt ctttgctttc gttaactgtt 2100 tggaggaaga atctttgtta ttgcatttaa tctcgaaatt cagagtgcac acctttctcc 2160 tgtaaagaaa cctgaagttg ctaccttat aggacagag aagtatccat cacgaaagac 2220 gggatcgcag tctttatgat tcatagtaat agttagttcc gacgttgaga tggattcgct 2280 gagaccggta gtggtcgtcc gagtacacga tgtgtcgtta actggataca ggttaatttc 2340 cacatcgata tagttaaacg tatttctggg tacgggttcg catttatctg cggagaagac 2400 ggtgtgagaa tatgttccga gaccacacgg agaacagatg acgtctccgg atactccgta 2460 tcctattcca cattttgttt gggaaacaca tgccttgcat ccggatgatc ctttgagaag 2520 acaataatat ccgggagagc attcacagat tctattgtga gtagtgttac acgatcgcat 2580 cttccgttac aacttagaca agcgggtaaa tgattattgc gagatgtgaa ggtacccgaa 2640 ccacacggcg tacattgtgt gttagtcttg ctatcgcata atctggaagc gtatgttccc 2700 ggacacaaat tatggcgttt gtattcgttg tctttacact ttccatcgga tggtgcatgc 2760 ggtgctatat ctcttccgtt tattattata catgagagaa acaatatata cgagtataat 2820 acggacttca taatttaata atgtagtaat cgttgtcttg ttcctgtttc ctacttctcc 2880 aatcatatag atattttctt tctatcatgg ataatatttg taatggttct ttccgtacaa 2940 catactgttt agatgatatt gcgcataatt tccggaggca aatacgatag tctagattgg 3000 ccatcgatgg tagactctaa tttattgagt gctttgtcga cgagtttact tttacgctcc 3060 atcgatagat ggcactgttc tatgagatcg tcgtacatgg gaaatgaaat gtgactgtct 3120 gaatgtatgg ctttaagata gctgtgatac cgtatacagg tcggtgtcgg agattcgaat 3180 ctctttaagg cgacttatgt cacgatgatg gaatctatct tatcgaatga tatattttc 3240 ataaatacac ttttatagtc ctcgtttaaa cagaatttac tatgtagttc cgcgaatgac 3300 tcgtccctta ataggcagta ggctagtatc ttttttacgt agtaatcgtc gtagggagag 3360 aattctgaca tcttgtagaa caacgattta atcataggta ctttcagtct gtggtggatg 3420 atgtcattca caacatccgc cttgtatatg atgtttctgt tttcaaacac caagtcgaat 3480 accgtcttta gtcggaaggt tgatgtcgta tccgatgtat gaggcaacat tgttgttaca 3540 attttgaaag gcggtattat aatattcgtc tttctgaatg tcgaacctat ctagtagata 3600 ccgtagtata ttgagagtgt atccttgatt atgttttatg aatagataaa gtagatgttg 3660 tccttcttcc ttttgttcgt gccaattgag taacattatg agaatatgac ctgttgcaca 3720 atcgttccat gatgggtgta caatcaagat tattacgtat cctcgagata aaagagcata 3780 caccacacga ggactatgtt tggtatactg ttgaaggtaa gtgtgtaacg gcgttaatgt 3840 ttgctccata atctattatc gcgtagatga atcgcttctc ggctcgcatc ttagtgtgac 3900 ttgacttgta ataattgctt ttgtagaacg tggatatgtg tttacagtag taatgaagag 3960 aagtgagttc atcctcgtcg gcgcaattag ggtcggatcc tttgtacaga acgtaatagt 4020 ttaagctccc attgaattta tatctagat aacacagcaa tattcggat gatttacta 4080 agtcatcaat gtgtccgtt agtatatcaa agatcttgtt atcgattgat agtggtcatc 4140 cttgctatca aagttacgca tgccgtggtg taacaatc tttatacag atggattaaa 4200 tcgtgtattc atcgtatagc aatgtaatgg agagttacct cgtttattca gatcgcagtg 4260 tttaatact agcttaaca gatgagacga tgtatccaca tcaagaacg windows 4320 atgacaaaca ttgttgacag aaacgtgacc ttcattctta ccgtcgtcca taaatacgtt 4380 aggtatgtac cacatactgt cgcgaacgat gcgtacaatc tcgtccatct cataatgatt 4440 tactttttca taattaaga tgtgaaagaaaccgaaca tattatttt tagtaatgtt 4500 tatgcgagac ataaaata aactccgtgt ttatgatgcc ggtaaatgtt tttatcatct 4560 tggacggaat cgatttgta atatgtcatg gaacaatg aaacaggaca ttatcgctcc 4620 atgataaattt atttaatgga gtaataagt atctccatgg gtaatttcga atcaagtta 4680 tcgtctgtat taatgttgtc cactatggag tcgatcctct cattgttctt tacagtttct 4740 gtaatgatgg acgttagttc ttttttgtac catttgatgt cggattcttt gcgtatctca 4800 atctgtggcg tttgcttcgt ttaaataata tatcaaacat ggagacgcct gatatgtagg 4860 cattcttcat tctattaatg tctactctat agcgctttag ttccttatga cgaccggcga 4920 tatcatactt tactttagaa ggaaaatcat catctatgat taaggcgtat ctgatacagg 4980 cgaataatgg ttcaggatat agatagcgta tatctctatt aaatgcgtca atcatagtct 5040 ctagagtggg atggtagcta agtaataaat caactagctt cgttttgttt tctcttctgt 5100 aactactttt ctggatggcc gtattgatta tcgagcgtga tgttgtaaca ctcgctccat 5160 attccaataa ccgctttgca aattgtatat tattgacatc gaccgcgtaa tatagtagag 5220 ttttattctc attatcgatc atatctatat tatccatgta cttgcttagt atatcaaata 5280 catctattag tatggtttca taacagtgat acccgcaatt attaaatctc gataatatca 5340 gaccgtacat acatagacgg ccattgttag atatgtgatt tacagccgcg tgtccatatt 5400 ttccacgata aaccttacga cgtttacatc gacgagatta ttattaacaa agtagtcgtg cagaggatag ttgttgtccg ttatctaaca tgcatcgaac gaccatatcg ccgtaatgta agtagtttat caacatggct tgtacgatgg attcatcctg ttgtctaaat ctctttagaa tgttatcgat gatgtagtgg ttatattctc tggaatcgta cgaagtaata ctacgcatta cgtcgacaag ctctcaata ctctcaata 5700. 5700. cgtcgacaag ctctcaata ctctcaata atggggttac tctaaatcgc ttgtttagat aatacgcctc taatataggg ctgacgtcgt atactctaca cgtgtccaca tcctttatta fatherattt aacaatctct atatctatgg ttgagaaaga ccagtagtat tggatgggta aagatcctcc ttcgtctctg ccatggatgg 5880 aaacattgtt attgatcaaa catttatta catccttgga tagattga gattctctat gagacgatat atagtaatga agagagttct actgttgtac actgttgtac actgttgtac gaatacgta accggtgctg taacattctg atttaagaag ccatagcaat acttctggtc tcggattagg cgtcgttacg fathers ccaatccgag accattgatt gcataattcg tattcttgga cggacgtatc cgttatcca caattaggta ttttagcaga cgtaagtcga 6180 tattatccga atacagatcg aaatcattta tattcgactt gagttcgtta gaggaattcg 6240 atagctgga tatcagtaga tgcacaattt gagatttgac gtatctatgc ttactgtata 6300 ctcctagcgg agttaatcct tcgttgtttc tacaaagtct ctcgactccg cgagagagta 6360 acagtcgaac aatcttaatg tctgtatcgc atttattgaa gacgtaacaa tgtagcgcat 6420 tgtttcctcg tctatctata tgttttgata agttgtgaca cgtttcaatt tctagtttta 6480 ttttttgta cgtcacatct tcatccagta gacgacatag aatacatgtg caatccatag 6540 ctattctggt gctaattatt cctatttcac gaaaaatgat gaaggcaatc attcctcata 6600 agatgataaa aagtgtagtg agagagcatg aaggagattt agtatttagc agtgcggata 6660 tgatccaaga gggtgagata gtcgttctcg ttcagaatct ttcgcagcat aagtagtatg 6720 tcgatatact tatcgttgaa gactcttcca gagacgatag ctgattgagt acaaagtcca 6780 atgattgcac gaagttcttc ggcggttttc atggagtcat ttctgatgaa acatttaatg 6840 atctccacgc aattgcgat attgcccac ggaagtgaat ccttcaactc accaccaaag 6900 agctccgttg catcagttct gaaagagatg agaagcctgt agagaggccc tgcagtttct 6960 ctatgggtcc atctatgaga aacccacagg atgtattcag tcagacaatg tctgacgtcg 7020 gccacggtat tcagggagtc cttagtagcg tggcaatgac agggtctgaa ctgggcacaa 7080 ggaaaggcca ttgtaaaggt agacctgtag ccgtttatgc tatagaggg ctttaatttc 7140 catttttta atgggttgtg gatgaggaat gagagtgata tcatattgag atacgtagtt 7200 atgtagaggt gtatttccta tattatttac tttcggttc atattttacc aactctttaa 7260 taaatttct ttcacgatgc atcttattaa atgacgtttt ctcataagtg gacatataga 7320 tgcagaagta atgaagaaaa gtattacctc tatcatctgc ataattaggg tctgctcctt 7380 ttcttaacaa cttatacagt acgtagtagt ttatcggttt taaatcaagt ctagaatata 7440 tagtggatta atatattttt atattagcta aagctatcta tactatcaga aagcatatca 7500 ttctcaactt catcatgagt taatatttg tgtaatggaa tgtgaccatc actgtcatga 7560 catactcctt taataggtt tttaaacag atgattcaa tccttcattc attagataac 7620 agtgtaacgg agtcgtacct tctagtt tgtttatatc acagcattct acaacagtc 7680 taaacaatag agaagacgga cagactttaa cgtataaatg acacatgtta tcgatattcg 7740 ttgatgaatt attattaac gtagttatga taaatgatc taacgacatc tctcgctaga 7800 gataaaatct agtatcgtat cattaaacat ctttgcatca tactcgcata gcatagtttt 7860 tcaatattaa tacaatattt aaagactta ttcggaagt attttatac atgtatcatc 7920 gatggagatc catatgagga gtcacttgta gttcttcagt agtaataca gtgctatcat 7980 cgatagtata atatatgta gaaggttcat atgttgttgt attggagta actgttgta 8040 gttcttccgt ggaatcaata attackactaa cagcaatagt atattatat aaatatgttc 8100 cgttgatatc acatatttta atgactcat ttctaacacc ctcagctata tctgtccaat 8160 taaatgtagc cacaatcta ctacgttctc tttgattgac tacttgtacg gtagcgacgc 8220 tacactatct ttattgtctt ctacatgctc cattgaatg tcatgataca acgcagtttt 8280 tcttatgcat gttcataac accacgaaca tgtcgcagta agatatatag ccagagataa 8340 tttctgtaaa ttcatgatg ccggtcataa acagcccgt caataattgt ggctatatat 8400 tcagtttata gagcaaata attaagcaca atagcgctta atctcaaat atgttatgtt 8460 tattttttc atattaaca tactggttaa aatccttata aggctct tcatctataa 8520 atcaagatca taattacatt tagacagtgg ttcatgtttt aaaaatgt tctttttgtg 8580 tgaatagga atatactaat caataatcaa ccatcgaccc cattacgata gtatgcaggc 8640 aacccccat tagagaggta cgtgtaatca gtctctccag ttttagttatt tttataagtc 8700 attgttacat aaacggcttt taaacagtct cctcgatat aagccatatc tggaattta 8760 ttaatactc gagtcatttt acgcacggtc aaaaagtaa gtaatgtcga cgacttctta 8820 cattctatag aaacacctag atactcatt ttctttgga aaatatccctc agactctgat 8880 ttgaacaatg cacgacctat agtaaaccgt gaccaataag ttatattagt caatggtata 8940 tccaaaccat caggtgtgga tagtacgccg atagtccagt ctttggtatc gatagtgtag 9000 ttattgaact gagaagttac cgtatagtct ttttggtcat ctctaaacaa ggaaactaat 9060 acctctacac tattgaacga tttatcttcc gtaatgggtg gaataacggg aataaagt 9120 ggactagcga tggatgaagt cacgaatata agacacgcta ttaatccgta tatcatcatt 9180 ttgatattac ttataataac gatttgttta atttttagtt tatactatta attgtaaatg 9240 atattattat ttttttaagt attatcagct ttagtttata ctatttgtaa tatttagaca 9300 tagataaacg tgataaaagt ctatttgttt atatttattg cggatagcag tatttcccta 9360 taaaaagtat acgtcctgtg ttgtctttaa tcatgtacat gaatggatgg tttatgtaga 9420 ccttcgtacg atataccatc gaaaagttag tcataaatac tcctgtaacg gccgatgctt 9480 ctgtatactc ctcattaaca tctataaacg tcgtatgtag aaatttttct acagtgatag 9540 tttcattaca catcttgcta aaatctgcat aatatccgaa tatattagta agtcctaaat 9600 tttctagaat aggtaccaga ttatacggtt ctgtcatttc cactttaaac tttggcatat 9660 acaagtctat acttttagta gataacatac cacaccattt tttaaatttt tcatctgtta 9720 tattttttc tattgttatat ataccttcta tgtcgtccgg tagtataatc accatactag 9780 agtttccctc gtatggaata tcgaataag agaatcctcc gaataattca ttaatatgta 9840 catattgcaa gttattctcg gtacccacca tcatatcac gctggtaact atattcttag 9900 aaatataaa cttgtctgta tatgtagat gtttagaaaa tggatatttc cacattgctt 9960 taaaatggac ggcgctaca actgtcatac gagtattaat ggatagcgga ctagtcaata 10020 aggaattaat tttaccattt gtcattgtct taacccattc gttgattagt tccttttgttt 10080 ggttagcatt attaaagttt acagtttga aatcgctttt tattttttgt aggaaggagg 10140 catggaactc gatactacg ctaccgtata ttttattgc ggtagctagt gtcgcacaat 10200 acggaatatc tacgtccatg tcattattgt catccttctt atcatcgggt gtattctcat 10260 tcatattctc tatatatttt gatagttgtt cagctgtaga accagctgct ccatgattta 10320 gagaata agtagataa atggaactg gagaatca aacatttca tcagggtgtt 10380 ttacgattag ttctttaag atatccatgg tatagaccaa acaatacga taacgatata 10440 tatcataaat aaataatgtt aaatttcagt ttatgtttgt accccgtatt catacttaac 10500 aaattggtat tacataccat taataatgca agcataaaa atcgttagta gatgttttcta 10560 atataggtt ccgtaagcaa agatataag atgaagggt atgataaaa tcaatcgtta 10620 tctaaaatga tcatactcat ttattttatt ctattatatt aacacataca ttttacag 10680 spider atattgtat tgttattttt atatttta spider atatttat 10740 tagtttatat tactgaatta ataatataa attcccaatc ttgtcataaa cacacactga 10800 gaaacagcat aaacacagaa tccatcaaa atgtcgatga atatctgat gttgttgttc 10860 gctgctatga taatcagatc attcgccgat agtggtaacg ctatcgaac agamttgcca 10920 gaaattacaa acgctacaac agatattcca gctatcagat tatgcggtcc agagggagat 10980 ggatattgtt tacacggtga ctgtatccac gctagagata tcgacggtat gtattgtaga 11040 tgctctcatg gttatacagg cattagatgt cagcatgtag tattagtag ctatcaacgt 11100 tcagaaaaac caacactac aacgtcatat atcccatctc ccggtattat gcttgtatta 11160 gtaggcatta ttattatgtg ttgtctatta tctgtttata ggttcactcg acgaactaaa 11220 ctacctctac aagatatggt tgtgccataa ttttataaa ttttttt gagtattttt 11280 aaaaatgt aaagtgta tgtcttatgt atatttataa aaatgctaaa tatgcgatgt 11340 atctatgtta tttgtattta tctaacaat acctctacct ctagatatta tacaaaatt 11400 ttttattca gcatattaaa gtaaaatcta gttaccttga aaatgaatac agtgggtggt 11460 tccgtatcac cagtaagaac ataatagtcg atacagtat ccgattgaga tttgcatac 11520 atactagtc tagaaagaaa tttgtaatca tcttctgtga cgggagtcca tatatctgta 11580 tcatcgtcta gtttatcagt gtcccatgct atatttctgt tatcatcatt agttaatgaa 11640 aaactctc gtgcttcaga aaagtcaat attgtatcca tacatacatc tccaaaacta 11700 tcgcttatac gtttatcttt aacgatacct ggttattac taacagacat 11760 tttccagatc tattgactat aactcctata gttccacat caaccaagta atgatcatct 11820 attgttatat aacaataaca taacttttt ccattttat cagtatgtat atctatatca 11880 acgtcgtcgt tgtagtgaat agtagttatt gatcttat atgaaacgga tatgtctaga 11940 acggcaattg tcttacgtcc agttacact ttctttgat taaagtctag agtctttgca 12000 aacatatat ccttatccga ctttatattt cctgtaggt ggtataattt tattttgcct 12060 ccacatatcg gtgtttccaa atattact agacaatatt ccatatagtt attagttaag 12120 ggtacccaat tagacacgt acgcttatta tcatcattg gatcgtattt cataaagtt 12180 attgtgttat cgatgtcaac acatctaca tttttaatc gtctatatag tattttctg 12240 atatttcta taatatcaga attgtctcc atcggagtt gtatactc ggaatcagtt 12300 acatgtttaa atattctct gatgtcattc cttatacaat caattcatt atttaacagt 12360 ttaatagtct gtagaccttt atcgtcgtaa atatccattg tcttattagt tacgcttatt 12420 tttatgtgtt tttacgttga tttattatat ttataagaa tgattgttg acgaatcacg 12480 agaactatta agacacatta ttaggtatat attaaaaa agttttgat tacgatgtta 12540 Window stack windows helpcast ctacattctt atacatcgt 12600 aatcaaaga attgcaattt tgatgtataa caactgtcaa tgggttatgg aattgtatat 12660 tacatattat acggtatgtt ggtaacgaca ataccgatc ggtaattgtc tgccggtgta 12720 atagaattat atattactat ctattacacc ggccttgtat acatataat aagttgtggt 12780 agtatgatct ccatatttat aatttaggac ttgtattca gtatttttgg aatcataaaa 12840 aaaaaaaa agttttacta atttaaaattt taaaaagtat ttacatttt ttcactgtttt 12900 agtcgcggat atggaatttcg atcctgccaa atcaataca tcatctatag atcatgtaac 12960 atattaca tacatagatg aaccaatga tatagacta acagtagca ttccgaaa 13020 tattatatca atacacaa aaaatcca cattttggcta atcaatttcg ggcttggaaa 13080 aaacgtatcg ccggaagga ctatatgact aacttacta gagatacagg atacaaca 13140 tcaaactta ctgaaactat acgtaactgt CAaaaaata gaacatata tggtctatat 13200 atacactaca atttagttat tattggata accgatgtga ttcaatca atttagaa 13260 ggttggtaaa ttggtacata gctataata cctatacacc cataataca acaaccattt 13320 ctgagttgga tatcatcaa atactgata atacgagga cgtgtataga gtaagtaag 13380 aaaaagaatg tggaatttgc tatgaagttg tttactcaa acgatagata ctttggtttta 13440 ttggattcgt gtaatcatat attttgcata acatgtatca atatatggca taaaacacga 13500 agagaaaccg gtgcgtcgga taattgtcct atatgtcgta cccgttttag aaacataaca 13560 atgagcaagt tctataagct agttaacta taaataaaa gtttaatttg ttgacgacgt 13620 atgtcgttat tttctcgta tgaagatta aattcaatc attcgttgt ttctaatata 13680 atctgccgta ttggatggat tctcagaca attgcattta gattatatca taataaaaa 13740 tagtagcaca caacacttc agcaacatt ctttttatata atgccatcta tcgtagtgag 13800 gacacaagtg aacctataat gagcaattt attagtatcg gttacatgaa ggactttg 13860 tagagtgacg attctaccat ctatggtacg aacggtttca tcttctttga tgccatcacc 13920 cagatgttct ataaacttgg tatcctcgtc cgatctcata tcctttgcca accaatacat 13980 gtagctaaaa ttaggcataa atttcacaca tccagtgcaa cgaaattctc cagaagatgt 14040 tacgatgttt aggttaggac atttgatttc gtcggcatta acatatgggt gaacacacccc 14100 atacatgaag gcgatgagaa ataggattct catcttgcca aaatatcact agaaaaaatt 14160 tattatcaa ttttaaaggt ataaaaaata cttattgttg ctcgaatatt ttgtatttga 14220 tggtatacgg aagattagaa atgtaggtat tatcatcaac tgattctatg gttttatgta 14280 ttctatcatg tttcactatt gcgttggaaa tatatcata tgcttccaca tatattttat 14340 tttgttttaa ctcatatac tcacgtaatt ctggattatt ggcatatcta tgaataattt 14400 tagctccatg atcagtaaat attaatgaga acatagtatt accacctacc attatttttt 14460 tcatctcatt caattcttaa ttgcaaagat ctatatataatc attatagcgt tgacttatgg 14520 actctggaat cttagacgat gtacagtcat ctataatcat ggcatattta atacattgtt 14580 ttagcata gtagttatct acgatgttag atatttctct caatgaatca atcacataat 14640 ctaatgtagg tttatgacat atagcattt tcagcagttc aatgtttta gattcgttga 14700 tggcaatggc tatacatgta tatccgttat ttgatctaat gttgacatct gaaccggatt 14760 ctagcagtaa agatactaga gattgtttat tatactaac agccttgtga agagtgttt 14820 ctcctcgttt gtcaatcatg ttaatgtctt taggatagg taggcaatg tttagtac 14880 taagaatttgg gcaagcataa gatagtcac aaagaccct tttgtagta taagtgtaaa 14940 aattaaca ttcatagttg gatttacata ggtgtccaat cgggatctct ccatcatcga 15000 gataattgat ggcatctccc ttccttttt agtagatatt tcatcgtgta agaatcaata 15060 ttaatattc taaagtatcc gtgtatagcc tctttatta ccacagttcc atattccact 15120 agaggatat cgccgaatgt catatactca attagtatat gttggaggac atccgagttc 15180 attgttttca attcaaaa gatggtttcc ttcatttc tccatagtgg tacaatacta 15240 cacattattc cgtgaggctt tccattctcc aaaaacaatt tgaccaaatc tacatcttta 15300 ttgtatctat aatcactatt tagataatca gccataattc ctcgagtgca acatgttaga 15360 tcgtctatat atgaataagc agtgttatct attcctttca ttaacaattt aacgatgtct 15420 atatctatat gagatgactt aatatatat tgaagacctg tacaatagtt tttatctata 15480 aaagacggct tgattccgtg attaattaga cattaacaa cttccggacg cacatatgct 15540 ctcgtatccg actttgaata cagatgcaat acggtaccgc aatttcgtag ttgataatca 15600 tcatacgcgt atcagtactc gtcctcataa agaacactgc agccattttc tatgaacaaa 15660 tcaataattt taggaacagg atcattgtca ttacataat ttctataact gaacgatggt 15720 tttcacattt aacactcaag tcaaatccat gttctaccaa cacctttatc aagtcaacgt 15780 ctacattttt ggatttcata tagctgaata tattaaagtc attatgttg ctaaatccag 15840 tggcttctag tagagccatc gctatatcct ttaactttaa catgtctact atttgtgtat 15900 tcttctaatg gggtagctgt ctccaatttt tgcgtaatgg attagtgcca ctgtctagta 15960 gtagtttgac gacctcgaca ttattacaat gctcattaaa aaggtatgcg tgtaaagcat tattcttgaa ttggttcctg gtatcattag gatctctgtc tctcaacatc tgtttaagtt catcgagagc cacctcctca ttttccaaat agtcaaacat tttgactgaa tgagctactg tgaactctat acacccacac aactatgtc attaaatatc atgtcaaaaa cttgtacaat fathers fathers tgtttaatt ttaccagttc cagattttac acctccgtta accccactgg acgatcctcc tccccacatt ccaccgccac cagatgtata agttttagat cctttattac taccatcatg tccatggata aagacactcc acatgccgcc actaccccct 16440. 16440. tcacgagtac 16500. 16500. 16500. 16500. 16500. 16500. 16500. 16500. 16500 aagtctctag tcacaagagc aatactacca acctacacta ttatgatt atagtttcta tttttatagg aacgcgtacg agaaaatcaa atgtctaatt tctaacggta gtgttgataa acgattatcg tcaatggata cctcctctat catgtcgtct attttcttac tttgttctat 16680 taacttatta gcattatata ttatttgatt ataaaactta tattgcttat tagcccaatc 16740 tgtaaatatc ggattattaa catatcgttt ctttgtaggt ttatttaaca tgtacatcac 16800 tgtaagcatg tccgtaccat ttatttaat ttgacgcata tccgcaattt ctttttcgca 16860 gtcggttata aattctatat atgatggata catgctacat gtgtacttat aatcgactaa 16920 tatgaagtac ttgatacata ttttcagataa cgatttatta ttaccaccta tgaatagata 16980 cctgtgatcg tctaggtaat caactgtttt cttaatacat tcgatggttg gtaatttact 17040 cagaataatt tccaatatct taatatataa ttctgctatt tctggaatat atttatctgc 17100 17160 gccattatct attaaccatt ctactaggct gacactatgc gacttaatac aatgataaag 17220 tatactacat ccatgtttat ctattttgtt tatatcatta atatacggct tacaaagttt 17280 tagtatcgat aacacatcca actcacgcat agagaaggta gggaataatg gcataaattatt 17340 tattaggtta tcatcattgt cattattac aactaagtttt ccatttttta aatatactc 17400 gandaacttta ggatctctat tgccaaattt ttgaaatat ttattat gctttaatct 17460 atataatgta gctccttcat caatcataca tttaataca ttgatgtata ctgtatgata 17520 agatacatat tcaacaata gatcttgtat agaatctgta tatcttta gattgtgga 17580 tattaggata ttattacgta aactattaca caatctaaa ataaaacg tatcacggtc 17640 gatataatagt tgatcaacta tataattatc gattttgtga ttttctctcc taaactgttt 17700 acgtaaatag ttagataga tattcattag ttcatgacca ctatagttac tatcgaataa 17760 cgcgtcaaat atttcccgtt taatatcgca ttgtcaaga taatataga gtgtggtatg 17820 ttcacgataa gtataatac gcatctcttt ttcgtgtgaa attaatagt ttcacgtc 17880 cieatgta gcaataccat cttgtgacct agtaata taatataga gaactgtttt 17940 acccattcta tcatcataat cagtggtgta gtcgtaatcg taatcgtcta attcatcatc 18000 ccaattataa tattcaccag cacgtctaat ctgttctatt ttgatcttgt atccatactg 18060 tatgttgcta catgtaggta ttcctttatc caataatagt ttaaacacat ctacattggg 18120 atttgatgtt gtagcgtatt tttctacaat attaatacca tttttgatac tatttatttc 18180 tatacctttc gaaattagta atttcaataa gtctatatcg atgttatcag aacatagata 18240 ttcgaatata tcaaaatcat tgatattttt atagtcgact gacgacaata acaaaatcac 18300 aacatcgttt ttgatattat tatttttctt ggtaacgtat gcctttaatg gagtttcacc 18360 atcatactca tataatggat ttgcaccact ttctatcaat gattgtgcac tgctggcatc 18420 gatgttaaat gttttacaac tatcatagag tatcttatcg ttaaccatga ttggttgttg 18480 atgctatcgc attttttggt ttctttctag ggataacagg gtaat 18525 <210> 2 <211> 24931 <212> DNA <213> Vaccinia virus <400> 2 tagggataac agggtaattg tttaaatttt accagttcca gattttacac ctccgttaac 60 cccactggac gatcctcctc cccacattcc accgccacca gatgtataag ttttagatcc 120 ttttattacta ccatcatgtc catggataaa gataccac atgccgccac taccccttt 180 agagacata ttaatagac ttaggacaa gtttaacaat aaattaatc acgagtaccc 240 tactaccaac ctacactatt atatgatt agttctctatt tttacagtac cttaactaaa 300 gtctctagtc acaagagcaa tactaccaac ctacactatt atatgatt agtttctatt 360 tttataggaa cgcgtacgag aaatcaaat gtctaatttc taacggtagt gttgataaac 420 gattatcgtc aatggatacc tcctctatca tgtcgtctat ttcttactt tgttctatta 480 acttattagc attatatatt atttgatt aaaacttata ttgcttatta gcccaatctg 540 taaatatcgg attackaca tatcgttct tgtaggttt attackatg tacatcactg 600 taagcatgtc cgtaccattt attaattt gacgcatatc cgcaatttct tttcgcagt 660 cggttataaa ttctatatat gatggataca tgctacatgt gtacttataa tcgactaata 720 tgaagtactt gatacatatt ttcagtaacg atttatt accacctag ataagtacc 780 tgtgatcgtc taggtaatca actgttttct taatacattc gatggttggt aatttactca 840 gataatttc caatatctta atatataatt ctgctatttc tggaatatat ttatctgcca 900 gtataacaca atagtatata catgtaaacc catattttgt tattatta atgtctgtgc 960 cattatctat taaccattct actaggctga cactatgcga cttaataca tgataagta 1020 tactacatcc atgtttatct atttgttta taccattaat atacggctta caagtttta 1080 gtatcgataa cacatccaac tcacgcatag agaagtagg gatataatggc atatattta 1140 ttaggttatc atcattgtca ttatttacaa ctaagtttcc atttttttaa atatactcga 1200 CAACttagg atctctattg ccaattttt gaaaatatttt atttatatgc ttaaatctat 1260 ataatgtagc tccttcatca atcatacatt taatacatt gatgtatact gtatgataag 1320 atacatattc taacaataga tcttgtatag aatctgtata tcttttaaga attgtgata 1380 ttaggatatt attacgtaaa ctattacaca attctaaaat ataaacgta tcacggtcga 1440 ataatagttg atcaactata taattatcga tttgtgat ttctctccta aactgtttac 1500 gtaaatagtt agatagaata ttcattagtt catgaccact atagttacta tcgaataacg 1560 cgtcaaatat ttcccgttta atatcgcatt tgtcaagata ataatagagt gtggtatgtt 1620 cacgataagt ataataacgc atctcttttt cgtgtgaaat taaatagttt atcacgtcca 1680 aagatgtagc ataaccatct tgtgacctag taataatata ataatagaga actgttttac 1740 ccattctatc atcataatca gtggtgtagt cgtaatcgta atcgtctaat tcatcatccc 1800 aattataata ttcaccagca cgtctaatct gttctatttt gatcttgtat ccatactgta 1860 tgttgctaca tgtaggtatt cctttatcca ataatagttt aaacacatct acattgggat 1920 ttgatgttgt agcgtatttt tctacaatat taataccatt tttgatacta tttatttcta 1980 tacctttcga aattagtaat ttcaataagt ctatatcgat gttatcagaa catagatatt 2040 cgaatatatc aaaatcattg atatttttat agtcgactga cgacaataac aaaatcacaa 2100 catcgttttt gatattatta tttttcttgg taacgtatgc ctttaatgga gtttcaccat 2160 catactcata taatggattt gcaccacttt ctatcaatga ttgtgcactg ctggcatcga 2220 tgttaaatgt tttacaacta tcatagagta tcttatcgtt aaccatgatt ggttgttgat 2280 gctatcgcat tttttggttt ctttcatttc agttatgtat ggatttagca cgtttgggaa 2340 gcatgagctc atatgatttc agtactgtag tgtcagtact attagtttca ataagatcaa 2400 tctctagatc tatagaatca aaacacgata ggtcagaaga taatgaatat ctgtaggctt 2460 cttgttgtac tgtaacttct cgttttgtta gatgtttgca tcgtgcttta acatcaatgg 2520 tacaaatttt atcctcgctt tgtgtatcat attcgtccct actataaaat tgtatattca 2580 gattatcatg cgatgtgtat acgctaacgg tatcaataaa cggagcacac catttagtca 2640 taacagtaat ccaaaatttt ttaaagtata tcttaacgaa agaagttgtg tcattgtcta 2700 cggtgtatgg tactagatcc tcataagtgt atatatctag agtaatgttt aatttatcaa 2760 atggttgata atatggatcc tcatgacaat ttccgaagat ggaaatgaga tatagacacg 2820 caataaatct aattgcggac atggttactc cttaaaaaaa tacgaataat caccttggct 2880 atttagtaag tgtcatttaa cactatactc atattaatcc atggactcat aatctctata 2940 cgggattaac ggatgttcta tatacggggga tgagtagttc tcttctttaa ctttatactt 3000 tttactaatc atattagac tgatgtatgg gtaatagtgt ttgaagagct cgttctcatc 3060 atcagaataa atcaatatct ctgttttttt gttatacaga tgtattacag cctcatatat 3120 tacgtaatag aacgtgtcat ctaccttatt aactttcacc gcatagttgt ttgcaaatac 3180 ggttaatcct ttgacctcgt cgatttccga caatctggg cgtataatga atctaaactt 3240 taatttcttg taatcattcg aaatattt tagtttgcat ccgtagttat cccctttatg 3300 taactgtaaa tttctcaacg cgatatctcc attaataatg atgtcgaatt cgtgctgtat 3360 acccatactg aatggatgaa cgaataccga cggcgttaat agtaatttac tttttcatct 3420 ttacatattg ggtactagtt ttactatcat aagtttataa attccacaag ctactatgga 3480 ataagccaac catcttagta taccacacat gtcttaaagt ttattaatta attacatgtt 3540 gttttatata tatcgctacg aatttaaaca gagaaatcag tttaggaaaa aaaattatct 3600 atctacatca tcacgtctct gtattctacg atagagtgct actttaagat gcgacagatc 3660 tgtgtcatca aaatatact ccattaaaat gattattccg gcagcgaact tgatattgga 3720 tatatcacaa cctttgttaa tatctacgac aatagacagc agtcccatgg ttccataaac 3780 agtgagttta tctttctttg aagagatatt ttgtagagat cttataaaac tgtcgaatga 3840 catcgcattt atatctttag ctaaatcgta tatgttacca tcgtaatatc taaccgcgtc 3900 tatcttaaac gtttccatcg ctttaaagac gtttccgata gatggcctca tttcatcagt 3960 4020 aaacgaatcg gctttattat acgcattcat gataaactta atgaaaaatg tttttcgttg 4080 gatgaatagt atgtcttaat aattgttat attttcatta attaatattt agtaacgagt 4140 acactctata aaaacgagaa tgacataact agttatcaaa gtgtctagga cgcgtaattt 4200 tcatatggta tagatcctgt aagcattgtc tgtattctgg agctattttc tctatcgcat 4260 4320 cgtctatatt aactctttta ttttctagcc atcgtaatac catgtttaag atagtatatt 4380 ctctagttac tacgatctca tcgttgtcta gaatatcaca tactgaatct acatccaatt 4440 ttagaaattg gtctgtgtta catatctctt ctatattatt gttgatgtat tgtcgtagaa 4500 aactattacg tagaccattt tctttataaa acgaatatat agtactccaa ttatctttac 4560 cgatatattt gcacacataa tccattctct caatcactac atctttaaga ttttcgttgt 4620 taagatattt ggctaaacta tataattcta ttagatcatc aacagaatca gtatatattt 4680 ttctagatcc aaagacgaac tctttggcgt cctctataat attcccagaa aagatatttt 4740 cgtgttttag tttatcgaga tctgatctgt tcatatacgc catgattgta cggtacgtta 4800 tgataaccgc ataaaataaa aatccatttt catttttaac caatactatt cataattgag 4860 attgatgtaa tactttgtta ctttgaacgt aaagacagta cacggatccg tatctccaac 4920 aagcacgtag taatcaaatt tggtgttgtt aaacttcgca atattcatca atttagatag 4980 aaacttatac tcatcatctg ttttaggaat ccatgtatta ttaccacttt ccattatccc 5040 aggctatgtt tcgtccatca tcgttgcgca gagtgaataa ttcttttgta ttcggtagtt 5100 caaatatata atccatgcat agatcggtaa agctattgta gatgtgattt ttcctaaatc 5160 tatataaaa ctcgtttact agcaaacact ttcctgattt atcgaccaag acacatatgg 5220 tttctaaatc tatcaagtgg tggggatcca tagttatgac gcagtaacat atattattac 5280 attcttgact gtcgctaata tctaaatatt tattgttatc gtattggatt ctgcatatag 5340 atggcttgta tgtcaaagat atagaacaca taaccaattt atagtcgcgc tttacattct 5400 cgaatctaaa gttaagagat ttagaaaaca ttatatcctc ggatgatgtt atcactgttt 5460 ctggagtagg atatattaaa gtctttacag attcgtccg attcaaataa attactaaat 5520 aatatcccac attatcatct gttagagtag tatcattaaa tctattatat tttatgaaaa 5580 atatatcact tgctcacctc tatatttcgt acatttttaa actgtttgta tatatctct 5640 ctgatatatt tattgtgtcg gtagacgata ccgttacatt tgaattaatg gtgttccatt 5700 ttacaacttt taacaagttg accaattcat ttctaatagt atcaaactct ccatgattaa 5760 atattttaat agtatccatt ttatatcact acggacacaa agtagctgac ataaaccatt 5820 gtataattt tatgttttat gtttattagc gtacacattt tggaagttcc ggctgccatg 5880 tatttcctgg agagcaagta gatgatgagg aaccagatag tttatatccg tacttgcact 5940 taaagtctac attgtcgttg tatgagtatg atcttttaaa cccgctagac aagtatccgt 6000 ttgatattgt aggatgtgga catttaacaa tctgacacgt gggtggatcg gaccattctc 6060 ctcctgaaca caggacacca gagttaccaa tcaacgaata tccactattg caactataag 6120 ttacaacgct cccatcggta taaaaatcct cgtatccgtt atgtcttccg ttggatatag 6180 atggagggga ttggcattta acagattcac aaataggtgc ctcgggattc cataccatag 6240 atccagtaga tcctaattca caatacgatt tagattcacc gatcaaatga tatccgctat 6300 tacaagagta cgttatacta gagccaaagt ctactccacc aatatcaagt tggccattat 6360 cgatatctcg aggcgatggg catctccgtt taatacattg attaaagagt gtccatccag 6420 tacctgtaca tttagcatat ataggtccca ttttttgctt tctgtatcca ggtagacata 6480 gatattctat agtgtctcct atgttgtaat tagcattagc atcagtctcc acactattct 6540 taaatttcat attaatgggt cgtgacggaa tagtacagca tgatagaacg catcctattc 6600 ccaacaatgt caggaacgtc acgctctcca ccttcatatt tatttatccg taaaaatgtt 6660 atcctggaca tcgtacaaat aaaaaaag cccatatatg ttgctattg tagaaattgt 6720 tttcacagt tgctcaaaaa cgatggcagt gacttatgag ttcacagtt agtaacata 6780 tcataatatt cgatattacg agttgacata tcgacaat tccaagtatt tgattttgga 6840 taatattcgt attttgcatc tgctatatt aagatatat caccgcaaga accacgaac 6900 atctttccta catggttaaa gtacatgtac aattctatcc atttgtctc cttaactata 6960 tattgtata gataattacg agtctcgtga gtaattccag taattacata gatgtcgccg 7020 tcgtactcta cagcataaac tatactatga tgtctaggca tgggagactt ttttatcha 7080 cgatttttag tgaacattc cacatcgtttt atactacat cgtggtataa 7140 actccaccca ttacatatat atcatcgtttt acgaataccg acgcgcctga atatctagga 7200 gtaattaagt ttggaagtct tatccattc gaagtgccgt gttcaata ttctgccaca 7260 cccgttgaaa tagaaatttc taatcctcct attacatata actttccatc gttaacacaa 7320 gtactaactt ctgattttaa cgacgacata ttagtaaccg ttttccattt ttcgtttta 7380 agatctaccc gcgatacgga ataaacatgt ctattgttaa tcatgccgcc aataatgtat 7440 agacaattat gtaaaacatt tgcattatag aattgtctat ctgtattacc gactatcgtc 7500 caatattctg ttctaggaga gtaatgggtt attgtggata tataatcaga gtttttaatg 7560 actactatat tatgttttat accatttcgt gtcactggct ttgtagattt ggatatagtt 7620 aatcccaaca atgatatagc attgcgcata gtattagtca taaacttggg atgtaaaatg 7680 ttgatgatat ctacatcgtt tggatttta tgtatccact ttaataatat catagctgta 7740 acatcctcat gatttacgtt aacgtcttcg tgggataaga tagttgtcag ttcatccttt 7800 gataatttc caaattctgg atcggatgtc accgcagtaa tattgttgat tatttctgac 7860 atcgacgcat tatatagtttt tttaattcca tatcttttag aaaagttaaa catccttata 7920 caatttgtgg aattaatatt atgaatcata gtttttacac atagatctac tacaggcgta 7980 acatcaatta ttacggcagc aactagtatc atttctacat tgtttatggt gatgttatc 8040 ttcttccagc gcatatagtc taatagcgat tcaaacgcgt gatagtttat accattcaat 8100 ataatcactt catcatttat atggtgctcc tgaatgcgtt taaaaaaatt atacggagac 8160 gccgtaataa tttccttatt cacttgtata atttcccat tgatagaaaa tatcacgctt 8220 tccattcttg aagtactata agtaattata gtataatgta aaggtttata tattcaatat 8280 tttttataaa aaaatcattt cgacattaat tccttttaa atttccgtct atcatctata 8340 gaaacgtatt ctatgaattt ataaatgct tttacgtgtc ctatcgtagg cgatagaacc 8400 gctaaaaagc ctatcgaatt tctacaaaag aatctgttat atggtatagg gagagtataa 8460 aacattaaat gcccgtactt attaaagtat tcagtagcca atcctaactc tttcgaatac 8520 ttattaatgg ctcttgttct gtacgaatct atttttttga acaacggacc tagtggtata 8580 tcttgttcta tgtatctaaa ataatgtctg actagatccg ttagtttaat atcctcagtc 8640 atcttgtcta gaatggcaaa tctaactgcg ggtttaggct ttagtttagt tttatatct 8700 acatctatgt ctttatctaa caccaaaaat samaagcta atattttatt acaatcatcc 8760 ggatattctt ctacgatctc actaactaat gtttctttgg ttatactgt atagtcacga 8820 tcagacaaat aaagaaaatc nagatgatcga tgaataatac atttaatttc atcatctgta 8880 agatttttga gatgtctcat taaatatta ttaggttag tactcattat cattcggcag 8940 ctattactta ttttatttt ttcaccata tagatcaatc attagatcat caaatatgt 9000 ttcaatcatc ctaaagagta tggtgaatga ctcttcccat ctaatttctg aacgttcacc 9060 aatgtctcta gccactttgg cactaatagc gatcattcgc ttagcgctt ctatattatt 9120 aactggttga ttcaatctat ctagcaatgg accgtcggac agcgtcattc tcatgttctt 9180 aatcaatgta catacatcgc cgtcatctac caatcatcc aacaata gctttttaaa 9240 atcatcatta taataggttt gatcgttgtc atttctccaa agaatatatc taataagtag 9300 agtcctcatg attagttaac aactattttt tatgttaat cattagtac accgctatgt 9360 ttaatactta ttcatatttt agttttagg attgagaatc atacaaaaa ttaatgcatc 9420 attaattta gaatactta gtttccacgt agttaatgaa acatttgaac tcatcgtaca 9480 ggacgttctc gtacaggacg taactataaa ccggtttata ttgttcaag atagatacaa 9540 atccgataac ttttttgacg aattctacgg gatccacttt aaagtgtca taccggttc 9600 tttttttt tttaaacaga tcaatggtgt gatgttgat aggtcttta cgaatttgat 9660 atagaatagc gttcacatat cctccataat ggtcaatcgc cattgttcg tatgtcataa 9720 attctttaat tatatgacac tgtgtattgt ttagttcatc cttgttcatt gttaggaatc 9780 tatccaaaat ggcaattata ctagaactat aggtgcgttg tatacacata ttgatgtgtc 9840 tgtttataca atcaatgata tttggatcca tgctactacc ttcgggtaaa attgtagcat 9900 catataccat ttctagtact ttaggttcat tattaccat tgcagaggac gtcatgatcg 9960 aatcataaaaatatatta tttttagtt attttgttaa aaataatcat cgaatacttc 10020 gtaagatact ccttcatgaa catagatact tacaaacgt tttatagag windowtactct 10080 acgattttta caaagtccg gatgcatag tacaagtac gcgaataacg gattaataat 10140 agatttatct agtcttactt ttctagc ttcatagtt agatcatgg ttcatagt 10200 aggattatgt aacatcagct tcgataaaat gactgggtta tttagtctta cacattcgct 10260 catacatgta tgaccgttaa ctacagagtc tacactaaaa tgattgaaca atagatagtc 10320 taccattgtt ttgtattcag atagtacagc gtagtacata gcatcttcac aaattatatc 10380 attgtctaat agatatttga cgcatcttat ggatcccact tcaacagcca tcttaaaatc 10440 atattgcttt cctttatcat tataatttc tagaacatca tctctatcat aaaagataca 10500 aatattaact gtttgatccg tataacatt gctagtcgat agcaatttgt tataagatg 10560 cgctaggctc aatgtcttaa taagaagtgt aagaggacta tctccgaatt tgttttgttt 10620 attaacatcc gttgatggaa gtaaaagatc tataatgtct acatacttaa ctgttttaga 10680 gcatacaata tggagaggtg tatttccatc atgatctggt tttgagggac taattcctag 10740 tttcatcatc catgagattg tagaagcttt tggattgtct gacataagat gtctatgaat 10800 atgattttg ccaaatttat ccactatcct ggcttcgaat ccgatggaca ttattttttt 10860 aaacactctt tctgaaggat ctgtacacgc caacaacgga ccacatcctt cttcatcaac 10920 cgagttgtta atcttggctc catactgtac caataaattt attctcta tgacttcatc 10980 atctgttccc gagagataat atagaggtgt tttatgt tattacacacg cgttttggatc 11040 tgcgccgtgc gtcagcagca tcgcgactat tctattatta ttaatttag aagctatatg 11100 caatggataa tttccatcat catccgtctc atttcctctat gagaagttc 11160 ttcgacaaat cgttcatcta gtcctttaat tccacaatac gcatgtagaa tgtgataatt 11220 atttccagaa ggttcgatag ctgtagcat attcctaaat acatctaaat ttttactatt 11280 atatttggca taaagagata gataactc ggccgacata atgttgtcca ttgtagtata 11340 aaaattaata tttctttc tattctgta tatttgcaac atttactct ctataacaaa 11400 tatcatact tagttctttt atgtcagaa ggcactggtt tagttcatct ataaatgtca 11460 cgccataact accacgcatg ccatactcag aattatgata aagatattta tccttggggt 11520 gtaggtaatg gggattaatc ttgttggat cagtctctaa gttaacacat gtcacacatg 11580 atccatttat agttatatca cacgatgatg atttatgaat tgattccgga agatcgctat 11640 cgtattttgt ggttccacaa ttcatttcca tacatgttat tgtcacacta atattgtgat 11700 gaactttatc tagccgctga gtggtaaaca acagaacaga tagtttatta tctttaccaa 11760 caccctcagc cgctgccaca aatctctgat ccgtatccat gatggtcatg tttatttcta 11820 gtccgtatcc agtcaacact atgttagcat ttctgtcgat atagctttca ctcatatgac 11880 actcaccaat aatagtagaa ttaatgtcat aatttacacc aatagtgagt tcggcggcaa 11940 agtaccaata ccggtaatct tgtcgaggag gacatatagt attcttgtat tctaccgaat 12000 acccgagaga tgcgatacaa aagagcaaga ctaatttgta aaccatctta ctcaaaatat 12060 gtaacaatag tacgatgcaa tgagtaagac aataggaaat ctatcttata tacacataat 12120 tattctatca attttaccaa ttagttagtg taatgttaac aaaaatgtgg gagaatctaa 12180 ttagtttttc tttacacaat tgacgtacat gagtctgagt tccttgtttt tgctaattat 12240 ttcatccaat ttattattct tgactatatc gagatctttt gtataggagt cagacttgta 12300 ttcaacatgc ttttctataa tcattttagc tatttcggca tcatccaata gtacattttc 12360 cagattagca gaatagatat taatgtcgta tttgaacaga gcctgtaaca tctcaatgtc 12420 tttattatct atagccaatt taatgtccgg aatgaaga agggaattat tggtgtttgt 12480 cgacgtcata tagtcgagca agagaatcat catatccacg tgtccatttt ttatagtgat 12540 gtgaatacaa ctaaggagaa tagccagatc aaaagtagat ggtatctctg aaagaaagta 12600 ggaaata cttacatcat taagcatgac ggcatgataa aatgaagttt tccatccagt 12660 tttcccatag aacatcagtc tccaattttt cttaacaaac agtttttaccg tttgcatgtt 12720 accactatca accgcataat acaatgcggt gtttcccttg tcatcaaatt gtgaatcatc 12780 cagtccactg aatagcaaaa tctttactat tttggtatct tccaatgtgg ctgcctgatg 12840 taatggaaat tcattctcta gaagattttt caatgctcca gcgttcaaca acgtacatac 12900 tagacgcacg ttattatcag ctattgcata atacaaggca ctatgtccat ggacatccgc 12960 cttaaatgta tctttactag agaaagct ttcagctgc ttagacttcc aagtattaat 13020 tcgtgacaga tccatgtctg aaacgagacg ctattagtg tatattttt cattttttat 13080 aattttgtca tattgcacca gatttaataa tatctctaat agatctgat agtagataca 13140 tggctatcgc aaaaacacat atacacatttt aaaaaata atatttatta agaaaattca 13200 gatttcacgt acccatcaat aataaaa taatgattcc tacaccgta cccatattaa 13260 ggagattcca ccttacccat aaacatata aatccagtaa tatcatgtct gatgatgaac 13320 acaaatggtg tattaaatttc cagttttca ggagatgatc tcgccgtagc taccatgata 13380 gtagatgcct ctgctacagt tccttgttcg tcgacatcta tcttgcatt ctgaaacatt 13440 ttaayatat ataatgggtc cctagtcata tgtttaaacg acgcattatc tggattaac 13500 attackaggag ccatcattc ggctatcgac ttatatccc tcttatttc gatagaaaat 13560 ctagggagtt taagattgta cactttattc cctaattgaa acgaccaata gtctaatttt 13620 gcagccgtaa tagaatctgt gaatgggtc atattatcac ctattgccag gtacatacta 13680 attack ccttatacgg aaggcgcacc attack cttcgtcatc gattgtgatt gtatttcctt gcaatttagt aactacgttc atcatgggaa ccgttttcgt accgtactta ttagtaaaac tagcattgcg tgttttagtg atatcaaacg gatattgcca tataccttta aaatatatag father tgcccataga gtattattgt cgagcatatt agaatctact acattagaca taccggatct acgttctact attachment ttttattaac cgcatctcgt ctaaagttta atctatatag gccgaatcta tgatattgtt gataatacga cggtttaata cacacagtat tatctacgaa actttgataa gttagatcag tgtacgtata tttagatgtt ttcagcttag ctaatcctga tattaattct gtaaatgctg gacccagatc tctttttctc aaatccatag tcttcaata ttctattcta gtattacctg atgcaggcaa tagcgacata aacatagaaa aacgaatacc aaacggtgag aagacaatat tatcatcttg aatattttta tacgctacta taccggcatt ggtaaatcct tgcagacgat aggtagacac tgaacacgtt aacgatagta tcaatacgc atcatgatt ttagttatt ataattac cttatttta 14400 tgttcggtat aaaaattatt gatgtctaca catccttttg taattgacat ctatatatcc 14460 tttgtataa tcactcta tcactcagtt ttcctacca gtttatccct 14520 atattcaaca tatctatcca tatgcatctt aacactctcc gccagatag cttcaaagtg 14580 aggatagtca aaagataaa tatatagagc ataatcattc tcgtatactc tgccctttat 14640 tacatcaccc gcattgggca acgaataca aaatgcaagc atcttgttaa cggggctcgta 14700 aattgggata aaattatgt ttttatct attttattca agagaatatt caggaatttc 14760 ttttccggt tgtatctcat cgcagtatat atcatttgta cattgtttca tattttttaa 14820 tagtttacac cttttagtag gactagtatc gtacaatca tagctgtatt ttgaattcca 14880 atcacgcata aaataatctt ccaattgttg acgaagacct atccatcat ccggtgtaat 14940 attaatagat gctccacatg tatccgtaaa gtaatttcct gtccaatttg aggtacctat 15000 ataggccgtt ttatcggtta ccatatattt ggcatggttt accctagaat acggaatggg 15060 aggatcagca tctggtacaa taaatagctt tacttctata tctatgtttt tagattttag 15120 catagcgata gatcttaaa agttctcat gataacgaa gatcgttgcc agcactaat 15180 caatagctta acggatactt gtctgtctat agcggatctt cttaattcat cttctatata 15240 aggccaaaac aaatttttac ccgccttcga attaata gggataagt tcatacaga 15300 tacataaacg atttactcg cattctaat acatgacaat aaagcggtta atcattggt 15360 tctttccata gtacatagtt gttgcggtgc agaagcaata atacagagt gtggacacc 15420 acttacgtta attackagag gatgatctgt attatac gacggataa agttttttcca 15480 attatatggt agattgttaa ctccaagata ccagtatacc tcaaaattt gagtgagatc 15540 cgctgccaag ttcctattat tgagatcgc atacccaat tctttgacct gagttagtga 15600 tctccaatcc atgttagcgc ttcctaaata atatgtgta ttatcagata tccaaaattt 15660 tgtatgaaga actcctccta ggatatttgt atatctatg tatcgtactt caactccggc 15720 catttgtagt ctttcaacat cctttaatgg tttgttagat ttattgacgg ctactctaac 15780 tcttactcct cttttgggta attgtacaat ctcgtttaat attatcgtgc cgaaattcgt 15840 acccacttca tccgataaac tccaataaaa agatgatata tctagtgttt ttgtggtatt 15900 ggatagaatt tccctccaca tgttaaatgt agacaaatat actttatcaa attgcatacc 15960 tataggaata gtctctgtaa tcactgcgat tgtattatcc ggattcattt tatttgttaa 16020 aaaataatcc tatatcactt cactctatta aaaatccaag tttctatttc tttcatgact 16080 gattttttaa cttcatccgt ttccttatga agatgatgtt tggcaccttc ataaattttt 16140 atttctctat tacaatttgc atgttgcatg aaataatatg cacctgaaac atcactaatc 16200 tcattgtttg ttccctggag tatgagagtc ggggggtgtt aatcttggaa attatttttc 16260 taaccttgtt ggtagccttc aagacctgac tagcaaatcc agccttaatt ttttcatgat 16320 tgattaatgg gtcgtattgg tatttataaa ctttatccat atctctagat actgattctg 16380 gacatagctt tccgactggc gcatttggtg tgatggttcc cataagtttg gcagctagca 16440 gattcagttt tgaacagca tctgcattaa ctagaggaga cattagaatc attgctgtaa 16500 acagttttgg attatcgtaa gaggctagta tagaaattgt tgctcccatg gatgaccca 16560 ataagtagat ttaatagtta ccacgtgctg taccaagtc atcaatcatc atttttcac 16620 cattacttct tccatgtcca atatgatcat gtgagaatac taaaattcct aacgatgata 16680 tgttttcagc tagttcgtca taacgtccag atgtttacc agctccatga cttatgaata 16740 ctaatgcctt aggatatgta ataggtttcc atatatgta atcattgtcc agattgaaca 16800 tacagtttgc actcatgatt cacgttatat aactacaat atttacagtt cgtttgatga 16860 tcatattatt tttagtttt attgaatatt gtaaaaacat acaattaat caatagag 16920 gaaggagacg gctactgtct ttgtgagat agtcatggcg actaaattag attgagga 16980 tgctgtttt tactttgtgg atgatgataa atatgtagt cgcgactcca tcatcgatct 17040 atagatgaa atattacgt ggagaatca tgttatagtg ttacaag atattaccag 17100 ttgtggaaga ctgtacaagg aattgatgaa gttcgatgat gtcgctatac ggtactatgg 17220. 17220. 17220. 17220. 17220. 17220. 17220. 17220 17280. cgctaccata ggaatatgtg ctaaaatcac tgaacattgg ggatacaaaa agatttcaga atctagattc caatcattgg gaaacattac agatctgatg accgacgat father cttgatactt tttctagaaa aaaaattgaa ttgatgatat aggggtcttc ataacgcata attacgt tagcattcta tatccgtgtt aaaaaaaatt atcctatcat gtatttgaga gttttatatg tagcaaacat gatagctgtg atgccaataa gctttagata ttcacgcgtg ctagtgttag ggatggtatt atctggtggt 17580. gaaatgtccg ttatataatc tacaaaacaa tcatcgcata tagtatgcga tagtagta aacattttta tagtttttac tggattcata catcgtctac ccaatttggt father attgtcgcca atcttacacc caaccccttg ttatccatta gtatagtatt aacttcgtta tttatgtcat aaactgtaaa tgattttgta gatgccatat catacatgat attcatgtcc ctattataat cattactaac tttatcacaa tatatgttga tatatctat atatgatcta 17880 gtctttgtgg gcaactgtct atacaagtcg tctaaacgtt gtttactcat atagtatcga 17940 acagccatca ttacatggtc ccgttccgtt gatagataat cgagtatgtt agtggacttg 18000 tcaaatctat ataccatatt ttctggaagt ggatatacat agtcgtgatc aacattattg 18060 ctagcctcat cttctatatc atgctata ccattatcta tatcatctac ataatctacg 18120 atattattac acataaacat cgacaacata ctattgttta ttatctaagt cctgttgatc 18180 caaacccttg atctcctcta tttgtactat ctagagattg tacttcttc agttctggat 18240 aatatatacg ttgatagatt agctgagcta ttctatctcc agtatttaca ttaaacgtac 18300 attttccatt attaataaga atgactccta tgtttcccct ataatcttcg tctattacac 18360 cacctcctat atcaatgcct tttagtgaca gaccagacct aggagctatt ctaccatagc 18420 aaatcttagg catggacata ctaatatctg tcttaattaa ctgtctttct cctggaggga 18480 tagtataatc gtagcgcta tacaaatcat atccggcagc acccggcgat tgcctagtag gagatttagc tctgttagtt tccttaacaa atctaactgg tgagttaata ttcatgttga acataaaact aatattttt ttcaaatta tttaccatcc catatattcc atgaataagt gtgatgattg tacacttcta tagtatcta attack cgataaaatc ctcctatcaa tagcagttta ttatccacta tgatcaattc tggattatcc ctcggataaa tagcatc 18840. 18840. 18840. 18840. 18840. 18840. 18840. 18840. 18840. 18840. 18840. 18840 taaccttcta ccgaacacta acgcgcatga tttataatga ggataatag tggatggtcc aaactgccac tgatcatgat tgggtagcaa atattctgta gttgtatcag tttcagaatg tcctcccatt acgtatataa cattgtttat ggatgccact gctggattac atctaggttt cagaagactc ggcatattaa cccaagcagc atccccgtgg aaccaacgct caacagatgt gggatttggt agacctccta ctacgtata tttattgtta gcggggtatcc cgctagcata 19140. cagtctgggg ctattcatcg gaggaattgg aatccaattg tttgatatat aatttacagc tatagcattg ttatgtattt cattgttcat ccatccaccg atgagatata ctacttctcc 19260 aacatgagta cttgtacaca tatggaatat atctataatt tgatccatgt tcataggata 19320 ctctatgaat ggatacttgt atgatttgcg tggttgttta tcacaatgaa atattttggt 19380 acagtctagt atccatttta cattatttat acctctggga gaaagataat ttgacctgat 19440 tacatttttg ataaggagta gcagatttcc taatctattt cttcgcttta tataccactt 19500 aatgacaaaa tcaactacat aatcctcatc tggaacattt agttcatcgc tttctagaat 19560 aagtttcata gatagataat caaaattgtc tatgatgtca tcttccagtt ccaaaaaagtg 19620 tttggcaata aagtttttag tatgacataa gagattggat agtccgtatt ctatacccat 19680 catgtaacac tcgacacaat attcctttct aaaatctcgt aagataaagt ttatacaagt 19740 gtagatgata aattctacag aggttaatat agaagcacgt aataaattga cgacgttatg 19800 actatctata tatacctttc atatacga gtaataatact atagaagtta aactgtgaat 19860 gtcaaggtct agacaaaccc tcgtaactgg atctttattt ttcgtgtatt tttgacgtaa 19920 atgtgtgcga aagtaaggag ataacttttt caatatcgta gaattgacta ttatttgcc 19980 acctatagca tcaataattg ttttgaattt cttagtcata gacaatgcta atatattctt 20040 acagtacaca gtattaacaa atatcggcat ttatgtttct ttaaaagtca acatctagag 20100 aaaaatgatt atctttttga gacataactc ccattttttg gtattcaccc acacgttttt 20160 cgaaaaaatt agttttcct tccaatgata tattttccat gaaatcaaac ggattggtaa 20220 cattataaat ttttttaaat cccaattcag aaatcaatct atccgcgacg aattctatat 20280 atgttttcat catttcacaa ttcattccta taagtttaac tggaagagcc gcagtaagaa 20340 attcttgttc aatggatact gcatctgtta taatatct aacggtttct tcactcggtg 20400 gatacaataa atgtttaaac atcaaacatg cgaaatcgca gtgcagaccc tcgtctctac 20460 taattagttc gttggaaaac gtgagtccgg gcattaggcc acgcttttta agccaaaata 20520 tggaagcgaa tgatccggaa aagaagaattc cttctactgc agcaaaggca ataagtctct 20580 ctccataacc ggcgctgtca tgtatccact tttgagccca atcggccttc ttttttacac 20640 aaggcatcgt ttctatggca ttaaagagat agtttttttc attactatct ttaacataag 20700 tatcgatcaa aagactatac atttccgaat gaatgttttc aatggccatc tgaaatccgt 20760 agaaacatct agcctcggta atctgtactt ctgtacaaaa tcgttccgcc aaattttcat 20820 tcactattcc gtcactggct gcaaaaaacg ccaatacatg ttttataaaa tatttttcgt 20880 ctggtgttag tttattccaa tcattgatat ctttagatat atctacttct tccactgtcc 20940 aaaatgatgc ctctgccttt ttatacatgt tccagatgtc ataatattgg attgggaaaa 21000 taacaaatct atttggattt ggtgcaagga tgggttccat aactaaatta acaatatcaa 21060 taaatttttt ttcagttatc tatatgcctg tacttggatt ttttgtacat cgatatcgcc 21120 gcaatcacta caataattac aagtattatt gatagcattg ttattagtac tatcataatt 21180 aaattatcga cattcatggg tgctgaataa tcgttattat catcattatc attttgtaat 21240 tgtgacatca tactaataa atcgtttgcg agattgttgt gggaagcggg catggaggat 21300 gcattatcat tattatta cgccttccat ttggattcac aaatgttacg cacattcac 21360 attttatgga aactataattt ttgtgaaac agatacaag aaactcgtc atcgttcaa 21420 ttttaacga tagtaaccg attaaacgtc gagctaattt ctaacgctag cgactctgtt 21480 ggatatgggt ttccagatat atatctttc agttccccta cgtatctata atcatctgta 21540 ggaaatggaa gatatttcca ttatctact gttcctaata tcatatgtgg tggtgtagta 21600 gaaccattaa gcgcgaaaga tgttattcg catcgtattt taacttcgca ataatttctg 21660 gttagataac gcactctacc agtcaagtca atgatattag cctttacaga tatattcata 21720 gtagtcgtaa cgatgactcc atctttaga tgcgatactc ctttgtatgt accagaatct 21780 tcgtacctca aactcgatat atttaaaca gttaatgaga tattaacgcg tttatgaat 21840 gatgatatat aaccagaagt ttatccctcg gtggctagcg ctataacctt atcattataa 21900 taccaactag tgtgattaat atgtgacacg ttagtgtggg tacaatatg tacattatcg 21960 tctacgtcgt attcgataca tccgcataca gccaacaaat ataaaatgac aaatactcta 22020 acgccgttcg tacccatctt gatgcggtt aataaatgtt ttgatttcaa tttattgtaa 22080 aaaaagattc ggtttatac tgttcgatat tctcattgct tatattttca tctatcatct 22140 ccacacagtc aaatccgtgg ttagcatgca cctcatcaac cggtaaaaga ctatcggact 22200 cttctatcat tataactcta gaatatttaa tttggtcatt attaatcaag tcaattatct 22260 tatttttaac aaacgtgagt attttactca ttttttataa aaacttttag aatatacag 22320 actctatcgt gtgtctatat cttcttttta tatccaatgt attatgtct gatttttctt 22380 catttatcat atataatggt ccaaattcta cacgtgcttc ggattcatcc agatcattaa 22440 ggttcttata attgtaacat ccttctctttc cctctctac atcttccttc ttattcttat 22500 tcttagcgtc acagaatcta ccacagcagg atcccatgac gagcgtcata ttaaactaat 22560 tcattttcaa ttataatata ctggtaatga ccattaaaat aaaaatattc ttcataaccg 22620 gtaagaaagt gaaaagttca cattgaaact atgtcagtag tatacatcat gaaatgatga 22680 tatatata ctctattttg gtggaggatt atatgatata attcgtggat aatcattttt 22740 aagacacatt tctttattcg taaatctttt cacgttaaat gagtgtccat atttgcaat 22800 ttcttcatat gatggcggtg tacgtggacg aggctgctcc tgttcttgtt gtggtcgccg 22860 actgtcgtgt ctgcgtttag atccctccat tatcgcgatt gcgtagatgg agtactatta 22920 tataccttgt attaaattt ttttattaat taaacgtata aaaacgttcc gtatctgtat 22980 ttaagagcca gatttcgtct atagaacaa atagctacag taaaaataac tagaataatt 23040 gctacaccca ctagaaacca cggatcgtaa tacggcaatc ggttttcgat aataggtgga 23100 acgtatattt tatttaagga cttaacaatt gtctgtaaac cacaatttgc ttccgcggat 23160 cctgtattaa ctatctgtaa aagcatatgt tggccgggcg gagccgaaca ttctccgata 23220 tccaatttct gtatatctat aatattatta acctccgcat acgcattaca gttctttct 23280 agcttggata ctacactagg tacatcatct agatctattc ctatttcctc agcgatagct 23340 cttctatcct ttccggaag catgaaatc acttcataa atgattcaac catgagtgtg 23400 aaactaagtc gagaattact catgcatttg ttagttattc ggaggcgcgca atttttaac 23460 tgtcctataa cctctcctat atgaatagca caagtgacat tagtagg agaatgttga 23520 gctaattttt gtaataact atcttaaaa agattataca aagttttaaa ctctttagtt 23580 tccgccattt atccagtctg agaaaatgtc tctcatata aatttcca agaaactaat 23640 tgggtgaaga atggaacct ttaatctata tttatcacag tctgttttgg tacacatgat 23700 gattcttct aatgctgtac taaattcgat atcttttcg atttctggat atgtttta 23760 taaagtatga aaagaat ggaatcgta ataccagtta tgtttaactt tgaaattgtt 23820 ttttatttc ttgttaatga ttccagccac ttgggaaag tcaagtcgt ttaatgccga 23880 tttaatacgt tcattaaaaa caactttt atcctttaga tgaattatta ttggttcatt 23940 ggaatcaaaa agtaagtat tatcggtttt aagatctgcg tgtaaaagt tgtcgcagca 24000 tggtagttcg taaattta tgtataacag agccatctgt aaaagataa acttttagta 24060 ttgtaccaaa gatttaaatc ctaatttgat agctaactcg gtatctactt tatctgcaga 24120 atacagtgct agggaaaa tattaatatt tcctcttcg tattcgtagt tagttctctt 24180 ttcatgttcg aaaagtgaa acatgcggtt aaatagtttt atacattaa tattactgtt 24240 aaactgcc gggtaaagt gggatagtaa ttcacgaat ttgatactgt cctttctctc 24300 gttaaacgcc tttaaaaaaa ctttagaaga atatctcaat gagagttcct gaccatccat 24360 agtttgtatc ataatagca acatgaag aacccgttta tacagagtat gtaaaaatgt 24420 taatttatag tttaatccca tggcccacgc accacgatt aatttttt catctccctt 24480 tagattgttg tatagaaatt tgggtactgt gaactccgcc gtagttttcca tgggactata 24540 taattttgtg gcctcgaata caattttac tacatagtta tctacttta agactatacc 24600 atatcctcct gtagatatgt gataaaaatc gtcgttatata ggataaaatc gtttatcctt 24660 ttgttggaaa aaggatgaat taatgtaatc attctctct atctttagta gtgttttccct 24720 attaaaattc ttaaaataat ttaacaatct aactgacgga gcccaatttt ggtgtaaatc 24780 taattgggac attatattgt taaaatacaa acagtctcct aatataacag tatctgataa 24840 tctatgggga gacatccatt gatattcagg ggatgaatca ttggcaacac ccatttattg 24900 tacaaaaagc ccctagggat aacagggtaa t 24931 <210> 3 <211> 23333 <212> DNA <213> Vaccinia virus <400> 3 tagggataac agggtaattc tttagtttcc gccatttatc cagtctgaga aaatgtctct 60 cataataaat ttttccaaga aactaattgg gtgaagaatg gaaaccttta atctatattt 120 atcacagtct gttttggtac acatgatgaa ttcttctaat gctgtactaa attcgatatc 180 tttttcgatt tctggatatg tttttaataa agtatgaaca aagaaatgga aatcgtaata 240 ccagttatgt ttaactttga aattgttttt tattttcttg ttaatgattc cagccacttg 300 ggaaaagtca aagtcgttta atgccgattt aatacgttca ttaaaaacaa actttttatc 360 ctttagatga attattattg gttcattgga atcaaaaagt aagatattat cgggtttaag 420 atctgcgtgt aaaaagttgt cgcagcatgg tagttcgtaa attttaatgt ataacagagc 480 catctgtaaa aagataaact ttatgtattg taccaaagat ttaaatccta atttgatagc 540 taactcggta tctactttat ctgcagaata cagtgctagg ggaaaaatta taatatttcc 600 tctttcgtat tcgtagttag ttctcttttc atgttcgaaa aagtgaaaca tgcggttaaa 660 atagtttata acattaatat tactgttaat aactgccggg taaaagtggg atagtaattt 720 cacgaatttg atactgtcct ttctctcgtt aaacgccttt aaaaaaactt tagaagaata 780 tctcaatgag agttcctgac catccatagt ttgtatcaat aatagcaaca tatgaagaac 840 ccgtttatac agagtatgta aaaatgttaa tttatagttt aatcccatgg cccacgcaca 900 cacgattaat tttttttcat ctccctttag attgttgtat agaaatttgg gtactgtgaa 960 ctccgccgta gtttccatgg gactatataa ttttgtggcc tcgaatacaa attttactac 1020 atagttatct atcttaaaga ctataccata tcctcctgta gatatgtgat aaaaatcgtc 1080 gtttatagga taaaatcgtt tatccttttg ttggaaaaag gatgaattaa tgtaatcatt 1140 ctcttctatc tttagtagtg tttccctatt aaaattctta aaataattta acaatctaac 1200 tgacggagcc caattttggt gtaaatctaa ttgggacatt atattgttaa aatacaaaca 1260 gtctcctaat ataacagtat ctgataatct atggggagac atccattgat attcagggga 1320 tgaatcattg gcaacaccca tttattgtac aaaaagcccc aatttacaaa cgaaagtcca 1380 ggtttgatag agacaaacta ttaactattt tgtctctgtt tttaatttct ttagtaatga 1440 aattattcac aatatcagta tcttctttat ctaccagaga ttttactaac ttgataacct 1500 tggctgtctc attcaatagg gtagtaatat ttgtatgtgt gatattgata tctttttgaa 1560 ttgtttcttt tagaagtgat tctttgatgg tgccagcata cgaattacaa taatgcagaa 1620 actcggttaa catgcaggaa ttatagtaag ccaattccaa ttgttgcctg tgttgtatta 1680 gagtgtcaat atgagcaatg gtgtccttgc gtttctctga tagaatgcga gcagcgattt 1740 tggcgttatc atttgacgat atttctggaa tgacgaatcc tgtttctact aactttttgg 1800 taggacaaag tgaaacaatc aagaagatag cttctcctcc tatttgtgga agaaattgaa 1860 ctcctctaga tgatctactg acgatagtat ctccttgaca gatttggac cgaattasag 1920 aagtacctgg aatgtaaagc cctgaaaccc cctcatttt taagcagatt gttgccgtaa 1980 atcctgcact gtgaccaaga tagagagctc ctttggtgaa tccatctcta tgtttcagtt 2040 taaccagaa acagtcagct gtctaaaat ttccatctct atctaataca gcatctact 2100 tgatgtcagg aactatgacc gttttaatgt tatatgtaac attgagtaaa tccttaagtt 2160 cataatcatc actgtcatca gttatgtacg atccaacaa tgtttctacc ggcatagtgg 2220 atacgaagat gctatccatc agaatgtttc cctgattagt attttctata tagctattct 2280 tctttaaacg atttccaaa tcagtaacta tgttcatttt tttaggagta ggacgcctag 2340 ccagtatgga agaggatttt ctagatccctc tctcacat ctttgatctc aatggaatgc 2400 aaaaccccat agtgaaacaa ccaacgataa aaataatatt gttttcact ttttatatat 2460 ttaccatctg actcatgat tcattatat ctttataga gctactacg tataatctt 2520 tataactgaa ctgagatata tacaccggat ctatggttttc cataattgag taatgaatg 2580 ctcggcaata actaatggca aatgtataga acaacgaaat tatactagag ttgttaaagt 2640 tatattttc tatgagctgt tccaataaat tatttgttgt gactgcgttc aagtcataaa 2700 tcatcttgat actatccagt aaacagtctt taagttctgg aattatca tcccattgta 2760 aagcccctaa ttcgactatc gaatatcctg ctctgatagc agtttcaata tcgacggacg 2820 tcaatactgt aataaaggtg gtagtattgt catcatcgtg ataaactact ggaatatggt 2880 cgttagtagg tacggtaact ttacacaacg cgatatataa ctttcctttt gtaccatttt 2940 taacgtagtt gggacgtcct gcagggtatt gttttgaaga aatgatatcg agaacagatt 3000 tgatacgata tttgttggat tcctgattat ttactataat ataatctaga cagatagatg 3060 attcgataaa tagaaaaggt atatcgttgg taggataata catccccatt ccagtattct 3120 cggatactct attaatgaca ctagttaaga acatgtcttc tattctagaa aacgaaaaca 3180 tcctacatgg actcattaaa acttctaacg ctcctgattg tgtctcgaat gcctcgtaca 3240 aggatttcaa ggatgccata gattctttga ccaacgattt agaattgcgt ttagcatctg 3300 attttttat taaatcgaat gtcggctct ctggttttgct acccaatga taacaatagt 3360 cttgtaaga taaaccgcaa gaaaatttat acgcatccat ccaatacc ctagcaccat 3420 cggatgatat taatgtatta ttatagattt tccatccaca gttattgggc cagtatactg 3480 ttagcaacgg tatatcgaat agattactca tgtacctac tagatgata gttcgtgtac 3540 tagtcataat atctttaatc caatctaaga atataaat tagacttttt acactgttaa 3600 agttaacaaa gttattaccc gggtacgtgg atatcatata tggtattggt ccattatcag 3660 taatagctcc ataaactgat acggcgatgg ttttatatg tgtttgatct aacgaggaag 3720 aaattcgcgc ccaattca tcttagata tgtatttaat atcaaacggt aacacatca 3780 tttcgggacg cgtatatgtt tctaaatttt taatccaaat ataatgatga cctatatgcc 3840 ctattatcat actgtcaact atagtacacc tagagaactt acgatacatc tgttttcctgt 3900 aatcgttaaa ttttacaaat ctataacatg ctaaaccttt tgacgacaac cattcattaa 3960 tttctgatat ggaatctgta ttctcgac cgtattgttc taaagccagt gctatatctc 4020 cctgttcgtg ggaacgcttt cgtataatat cgatcaacgg ataatctgaa gtttttggag 4080 aataatatga ctcatgatct atttcgtcca taaacaatct agacatagga attggaggcg 4140 atgatcttaa ttttgtgcaa tgagtcgtca atcctataac ttctaatctt gtaatattca 4200 tcatcgacat ataactatct atgttatcat cgtatattag tataccatga ccttcttcat 4260 ttcgtgccaa aataatatac agtcttaaat attacgcaa tatctcaata gtttcataat 4320 tgttagctgt ttcatcaag gtttgtatcc tgtttaacat gatggcgttc tatacgtttc 4380 tatttctat ttttaaatt tttaacgatt tactgtggct agatacccaa tctcttcaa 4440 atatttttt agcctcgctt acaagctgtt tatctatact attaaaactg acgaatccgt 4500 gattttggta atgggtccg tcgaaatttg ccgaagtgat atgaacatat tcgtcgtcga 4560 ctatcaacaa ttttgtatta ttctgaatag tgaaaacctt cacagataga tcattttgaa 4620 cacacaacgc gtctagactt ctggcggttg ccatagaata tacgtcgttc ttatcccaat 4680 taccaactag aagtctgatc ttaactcctc tattaatggc tgcttctata atggagttgt 4740 aaatgcggg ccaatagtag ctattaccgt cgacacgtgt agtgggaact atggccaaat 4800 gttcaatatc tatactagtc ttagctgacc tgagtttatc aataactaca tcggtatcta 4860 aatctctaga atatcccaat aggtgttccg gagaatcagt aaagaacact ccacctatag 4920 gattcttaat atgatacgca gtgctaactg gcaaacaaca agccgcagag cataaattca 4980 accatgaatt tttgcgcta ttaaaggctt taaaagtatc aaatcttcta cgaagatctg 5040 tggccagcgg gggataatca gaatatacac ctaacgtttt aatcgtatgt atagatcctc 5100 cagtaaatga cgcgtttcct acataacatc tttcatcatc tgacacccaa aaacaaccga 5160 gtagtagtcc cacattattt tttttatcta tattaacggt tataaaattt atatccgggc 5220 agtgactttg tagctctccc agatttcttt tccctcgttc atctagcaaa actattattt 5280 taatcccttt ttcagatgcc tcttttagtt tatcaaaaat aagcgctccc ctagtcgtac 5340 tcagaggatt acaacaaaaa gatgctatgt atatatattt cttagctaga gtgataattt 5400 cgttaaaaca ttcaaatgtt gttaaatgat cggatctaaa atccatattt tctggtagtg 5460 tttctaccag cctacatttt gctcccgcag gtaccgatgc aaatggccac atttagttaa cataaaaact fathercatcct gttctatcaa cgattctaga atatcatcgg ctatatcgct aaaattttca tcaaagtcga catcacaacc taactcagtc aatattaattaa gaagttccat gatgtcatct tcgtttattt ctatatccgt atccattgta gattgttgac cgattatcga 5700. gtttaaatca ttactaatac tcaatccttc agaatacaat ctgtgtttca ttgtaaattt ataggcggtg tatttaagtt ggtagtttt caattatgta tcaatatagc aacagtagtt cttgctcctc cttgattcta gcatcctctt cattattttc ttctacgtac ataaacatgt ccatacgtt agacaacaca ccgacgatgg cggccgccac agacacgaat atgactaaac cgatgaccat ttaaaaaccc ctctctagct ttcacttaaa ctgtatcgat tattctttta cattgtat fatheraaaa cattattcta tttcgaattt aggcttccaa aaatttttca tccgtaaacc the sword of the sword tgttaatagt the sword of the swordgc ttaaactatc atcatttcca cgattagaga tacaatattt acatttttt tgctgtttcg aaactttatc aatacacgtt aatacaaacc caggaaggag atattgaaac tgaggctgtt gaaaatgaaa cggtgaatac aatattcag atcatgattc cgtattctga tgatattaga actgctaatg gatgtcgatg gtatgtatct aggagtatct attttaacaa agcatcgatt tgctaatata caattatcct tttgattaat tgttatttta ttcatattct taaaaggttt catattatc aattcttcta cattaaaaat ttccattttt aattatgta gccccgcaat actcctcatt acgtttcatt ttttgtctat aatatccatt ttgttcatct 6540. cggtacatag attack tgagaagcgc attack tttgtacatt attack tgacaaatcg tcgaaaacta gttatagtta acattttatt atttgatacc ctgatattaa tacccctgcc gttactatta tttataactg atgtaaccca cgtaacatta gattatta tcgatagtaa tgcatcaacg cttccaaaat tgtctattat aaactcaccg ataatttttt tattacatgt tttcatattc attack tcaaatcttt aatcttacta cgattgtatg cgttgatatt gcaagacgtc attctaaaag acggaggatc tccatcaaat gccaaacaat cacgtacaaa gtacatggaa ataggttttg ttctattgcg catcatagat ttatatagaa 6960 cacccgtaga aatactaatt tgttttactc tataaaatac taatgcatct atttcatcgt 7020 tttgtataac gtctttccaa gtgtcaaatt ccaaattttt ttcattgata gtaccaaatt 7080 cttctatctc tttaactact tgcatagata ggtaattaca gtgatgccta catgccgttt 7140 tttgaaactg aatagatgcg tctagaagcg atgctacact agtcacaatc accactttca 7200 tatttagaat atatgtatgt aaaaatatag tagaatttca ttttgttttt ttctatgcta 7260 taaatgaatt ctcatttgc atctgctcat actccgtttt atatcaatac caaagaagga 7320 agatatctgg ttctaaaagc cgttaaagta tgcgatgtta gaactgtaga atgcgaagga 7380 agtaaagctt cctgcgtact caaagtagat aaaccctcat cacccgcgtg tgagagaaga 7440 ccttcgtccc cgtccagatg cgagagaatg aataaccctg gaaaacaagt cccgttatg 7500 agaacggaca tgctacaaaa tatgttcgcg gctaatcgcg acaacgtggc gtcgagactt 7560 ttgaactaaa atacaattat atctttttcg atattaataa atccgtgtcg cccgggtttt 7620 ttatctcttt cagtatgtga atagataggt attttatctc tattcatcat cgaatttaag 7680 agatccgata aacattgttt gtattctcca gatgtcagca tctgatacaa catatatgt 7740 gcacataaac ctctggcact tattcatgt accttcccct tatcactaag gagaatagta 7800 tttgagaaat atgtatacat gatatca tgattagat atacagaatt tgtaacactc 7860 tcgaaatcac acgatgtgtc ggcgttaaga tctatatat cactcgataa cacattttca 7920 tctagataca ctagacatttt tttaaagcta aaatagtctt tagtagtgac agtaactatg 7980 cgattatttt catcgatgat acatttcatc ggcattattt tacgcttacc atcaagact 8040 attackgtg tatatctaac gtattctagc atggttgcca tacgcgcatt aaacttttca 8100 ggatctttgg atagatcttc caatctatct atttgagaaa acattttat catgttcaat 8160 agttgaaacg tcggatccac tatatagata ttatctata agatttagg aactacgttc 8220 atggtatcct ggcgaatatt aaactatca atgatatgat tatcgttttc atcttttatc 8280 accatatagt ttctaagata tgggatttta cttaatataa tattattcc cgtgataaat 8340 tttattagaa aggccaaatc tataagaaaa gtcctagaat tagtctgaag aatatctata 8400 tcgccgtata gtatatttgg attaattaga tattagagaat atgatccgta acatatacaa 8460 cttttattat ggcgtctaag atattcttcc atcaacttat taacatttt gactagggaa 8520 gatacattat gacgtcccat tacttttgcc ttgtctatta ctgcgacgtt catagaattt 8580 agcatatctc ttgccaattc ttccattgat gttacattat aagaaatttt agaatgaaatt 8640 acatttggag ctttaatagt aagaactcct aatatgtccg tgtatgtggt cactaataca 8700 gattgtagtt ctataatcgt aaataattta cctatattat atgtttgagt ctgtttagaa 8760 aagtagctaa gtatacgatc ttttatttct gatgcagatg tatcaacatc ggaaaaaaat 8820 ctttttttat tcttttttac taaagataca aatatgtctt tgttaaaaac agttattttc 8880 tgaatatttc tagcttgtaa ttttaacata tgatattcgt tcacactagg tactctgcct 8940 aaataggttt ctataatctt taatgtaata ttaggaaaag tattctgatc aggattccta 9000 ttcattttga ggatttaaaa ctctgattat tgtctaatat tgtctctacg caaactttt 9060 cacagagcga tagagttttt gataactcgt ttttcttaag aaatataaaa ctactgtctc 9120 cagagctcgc tctatctttt attttatcta attcgataca aactcctgat actggttcag 9180 aaagtaattc attaatttc agtcctttat agaagatatt tatatagat aatacaaaat 9240 cttcagtttt tgatatcgat ctgattgatc ctagaactag atatattaat aacgtgctca 9300 ttaggcagtt tatggcagct tgataattag atatagtata ttccagttca tatttattag 9360 ataccgcatt gcccagattt tgatattcta tgaattcctc tgaaaataaa tccaaaataa 9420 ctagacattc tattttttgt ggattagtgt actctcttcc ctctatcatg ttcactactg 9480 gtgtccacga tgataaatat ctagagggaa tataatatag tccataggat gccaatctag 9540 caatgtcgaa taactgtaat tttattcttc gctcttcatt atgaattgat tcttgaggta 9600 taaacctaac acaaattata ttattagact tttcgtatgt aatgtctttc atgttataag 9660 ttttaatcc tggaatagaa tctattttaa tgaggcttt aaacgcagag ttctccaacg 9720 agtcaaagca tatactctg ttgtttttct tatatacgat gttacgattt tcttctttga 9780 atggaatagg ttttgaatt agtttatat tacacataa tagataag agtgtgcaaa 9840 tagtacgcgg aaaaaacata atagctcccc tgttttcatc catggtttta agtaaatgat 9900 cactggcttc tttagtcaat ggatattcga acattaaccg ttcatcatc attggacaga 9960 atccatattt cttaatgtaa agagtgatca aatcattgtg tttattgtac catcttgttg 10020 taaatgtgta ttcggttatc ggatctgctc ctttttct taaagtatcg atatcgatct 10080 cgtctaagaa ttcaactata tcgacatatt tcattgtat acacatacc attackacg 10140 tagaatgtat aggagagat gtaacgggaa cagggttgt tgatcgcaa actattctaa 10200 tacataattc ttctgttaat acgtcttgca cgtaatctat tatagatgcc aagatatcta 10260 tataattatt ttgtaagg atgttaacta tgtgatctat atagtagtg taataattca 10320 tgtatttga tatatgttcc aactctgtct ttgtgatgtc tagtttcgta atatctatag 10380 catcctcaaa aaatattc gcatatattc ccaagtctc agttctatct tctaaaaaat 10440 cttcaacgta tggaataata taatctattt tacctctct gatatcatta atgatatagt 10500 ttttgacact atcttctgtc aattgattct tattcactat atctaagaaa cggatagcgt 10560 ccctaggacg aactactgcc attaatatct ctattatagc ttctggacat aattcatcta 10620 ttataccaga attaatggga actattccgt atctatctaa catagtttta agaaagtcag 10680 aatctaagac ttgatgttca tatattggtt catacatgaa atgatctcta ttgatgatag 10740 tgactatttc gttctctgaa aattggtaac tcattttata tatgctttcc ttgttgatga 10800 aggataggat atactcaata gaatttgtac caacaaactg ttctcttatg aatcgtatat 10860 catcatctga aataatcatg taaggcatac attaacaat tagagacttg tctcctgtta 10920 tcaatact attcttgtga taatttatgt gtgaggcaaa tttgtccacg ttctttaatt 10980 ttgttatagt agatatcaaa tccaatggag ctacagttct tggcttaaac agatatagtt 11040 tttctggaac gaattctaca acattattat aaaggacttt gggtagataa gtgggatgaa 11100 atcctatttt aattaatgct atcgcattgt cctcgtgcaa atatccaaac gctttttgtga 11160 tagtatgaca ttcattatct agaaacgctc tacgaatc tgtcacagat atcatcttta 11220 gagaatacta gttgcgttaa tagtactaaa atttgtattt ttttatat atctcaaaa 11280 aaatttaat tttggatcc gatgtataat tagaatcaga atctaatgat gacgtaccca 11340 agaagtttat ctacagccaa tttagctgca ttattttag catctcgtttt agattttcca 11400 tcggccttat cgaatactct tcgtcgata tctacacagg cataaaatgt aggagagtta 11460 ctaggcccaa ctgattcaat acgaaagac caatctctct tagttattg gcagtactca 11520 ttaataatgg tgacaggtt agcatctttc caatcaataa ttttttagc cggaataca 11580 tcatcaaag acttatgatc ctctctcatt gatttttcgc gggatacatc atctattatg 11640 gcgtcagcca taaccagc atccggcttta tccgcctccg ttgtcataaa ccaacgagga 11700 ggaatatcgt cggagctgta caccatagca ctacgttgaa gatcgtacag agctttatta 11760 acttctcgct tctccatatt aagttgtcta gttagttgtg cagcagtagc tccttcgatt 11820 ccaatgtttt taatagccgc acacacaatc tctgcgtcag aacgctcgtc atatagatc 11880 ttagacattt ttagagagaa ctaacacaac cagcaataaa actgaaccta ctttatcatt 11940 tttttattca tcatcctctg gtggttcgtc gttcctatcg aatgtggatc tgattaaccc 12000 gtcatctata ggtgatgctg gttctggaga ttctggagga gatggattat tatctggaag 12060 aatctctgtt atttccttgt tttcatgtat cgattgcgtt gtaacattaa gattgcgaaa 12120 tgctctaaat ttgggaggct taaagtgttg tttgcaatct ctacacgcat gtctaactag 12180 tggaggttcg tcagcggctc tagtttgaat catcatcggc gtagtattcc tacttttaca 12240 gttaggacac ggtgtattgt atttctcgtc gagaacgtta aaataatcgt tgtaactcac 12300 atcctttatt ttatctatat tgtattctac tcctttctta atgcatttta taccgaataa 12360 gagatagcga aggaattctt tttcggtgcc gctagtaccc ttaatcatat cacatagtgt 12420 tttatattcc aaatttgtgg caatagacgg tttatttcta tacgatagtt tgtttctgga 12480 atcctttgag tattctatac caatattatt ctttgattcg aatttagttt cttcgatatt 12540 agattttgta ttacctatat tcttgatgta gtactttgat gatttttcca tggcccattc 12600 tattaagtct tccaagttgg catcatccac atattgtgat agtatattctc ggatatcagt 12660 agcggctacc gccattgatg ttgttcatt ggatgagtaa ctactaatgt atacattttc 12720 catttataac acttatgtat taactttgtt catttatatt tttcattat tatgttgata 12780 ttaacaaag tgaatatata tgttaataat tgtattgtgg ttatacggct acaatttcat 12840 aatgagtgga agtcagtgtc cgatgatcaa tgacgatagc tttactctga aaagaagta 12900 tcaatcgat attgcggagt acaatga aatgatag agagacaa agttcaga 12960 tagagccaaa atggtaaag aaataatca gatataga gcagcacaaa ctcattacga 13020 gattgaaa ctaggataca taaatttaa gagaatgatt atgatacta cttagaga 13080 tatagcacca tctattccaa ataatcagaa aacttataaa ctattctcgg acatttcagc 13140 catcggcaaa gcatcacgga atccaagtaa gatggtatat gctctgctgc tttacatgtt 13200 tcccaatttg tttggagatg accatagat cattcgttat agaatgcatc caatgagtaa 13260 aatcaacac aagatcttct ctccttttcaa acttaatctt attagaatat tagtggaga 13320 aagattctat aaatgaat gcagatctaa taatggaac acagttgat aaatgttga 13380 tagctgaatc tgataaatat acatagatg caagtataa cctaaaaccc atgtatagaa 13440 tcaagggaga atctgagaa gataccctct tcatcaaca gatggtagaa caatgtgtga 13500 catcccagga attggtggaa aaagtgttga agatactgtt tagagatttg ttcaagagtg 13560 gagaatacaa agcatacaga tacgatgatg atgtagaaaa tggattatt ggattggata 13620 cactaaaatt aacattgtt catgatatag ttgaaccatg tatgcctgtt cgtaggccag 13680 tggctaagat actgtgtaaa gaatggtaa ataatactt tgagaatccg ctacatatta 13740 ttggtaaaaa tcttcagag tgcattgact tgttga taggcattt catctttctc 13800 caatactaat tcaattgtt aattaata tggatagtat aaatagttat tagtgataa 13860 atagtaaaa tattattag aattaaggtg tagtacata gataactc ttcttaaaa 13920 atggatttta ttcgtagaaa gtatcttata tacacagtag aaaataatat agatttttta 13980 aaggatgata cattaagtaa agtaaacaat tttaccctca atcatgtact agctctcaag 14040 tatctagtta gcaattttcc tcaacacgtt attactaagg atgtattagc tataccaat 14100 ttttttgttt tcatacatat ggtacgatgt tgtaaagtgt acgaagcggt tttacgacac 14160 gcatttgatg cacccacgtt gtacgttaaa gcattgacta agaattattt atcgtttagt 14220 aacgcaatac aatcgtacaa ggaaaccgtg cataaactaa cacaagatga aaaattttta 14280 gaggttgccg aatacatgga cgaattagga gaacttatag gcgtaaatta tgacttagtt 14340 cttaatccat tatttcacgg aggggaaccc atcaaagata tggaaatcat ttttttaaaa 14400 ctgtttaaga aaacagactt caaagttgtt aaaaaattaa gtgttatag attacttatt 14460 tgggcttacc taagcaagaa agatacaggc atagagtttg cggataatga tagacaagat 14520 atatacactc tatttcaaca aactggtaga atagtccata gcaatctaac agaaacgttt 14580 agagattata tctttcccgg agataagact agctattggg tgtggttaaa cgaaagtata 14640 gctaatgatg cggatattgt tcttaataga cacgccatta ccatgtatga taaaattctt agttatatat actctgagat aaaacaagga cgcgttaata aaaacatgct tagttagtt father agcctgaaaa agatatcaga gaacttctgc tagatcat atatgatatt cctggagata tcctatctat tattgatgca aaaaacgacg attggaaaaa atattttatt agtttttata aagctaattt tattaacggt aatacattta ttagtgataa aacgtttaac gaggacttat tcagagttgt tgttcaata aatcccgaat atttcgataa tgaacgaatt atgtctttat tctctacgag tgctgcggac attaacgat ttgatgagtt agatattaat aacagttata tatctatat aatttatgag gtgaacgata tcacattaga tacaatggat gatatgaaga agtgtcaaat ctttaacgag gatacgtcgt attatgttaa ggaatacaat acatacctgt ttttgcacga gtcggatccc atggtcatag agaacggat actaaagaaa ctgtcatcta taaatccaa gagtagcgg ctgaacttgt ttagcaaaaa cattttaaaa tattattag acggacaatt ggctcgtcta ggtcttgtgt tagat taaggagac 15360 ttgttagtta aaatgataaa ccatcttaag tctgtggagg atgtatccgc attcgttcga 15420 ttttctacag ataaaaaccc tagtattctt ccatcgctaa tcaaactat tttagctt 15480 tataatattt ccatcatcgt cttattca aggttttga gagataatct atatcatgta 15540 gagaatttct tggataaag catccatcta accaagacgg atagaata tatacttca 15600 ttgataagac acggtagatc atagaacaga ccaaatatat tattaatat ttgtatatac 15660 atagatataa ttatcacata ttaaaaattc acacatttt gataatggg aactgctgca 15720 acaatcaga ctcccaccaa attaatgaat aaagaaaatg cagaatgat tttggaaaaa 15780 attgttgatc atatagttat gtatattagt gacgaatcaa gtgattcaga aaataatcct 15840 gatatattg atttcgtaa cagatacgaa gactatagat ctctcattat aaaagtgat 15900 cacgagtttg taagctatg taaaaatcat gcggagaaaa gttctccaga aacgcaacaa 15960 atgatttaca aacacatata cgaacaata cttattccag tatctgaagt actattaaaa 16020 cctataatgt ccatgggtga cataattaca tataacggat gtaaagacaa tgaatggatg 16080 ctagaacaac tctctaccct aaactttaac aatctccgca catggaactc atgtagcata 16140 ggcaatgtaa cgcgtctgtt ttatacattt tttagttatc tgatgaaaga taaactaaat 16200 atataagtat aatcccattc taatacttta acctgatgta ttagcatctt attagaatat 16260 taacctaact aaaagacata acataaaaac tcattacata gttgataaaa agcggtagga 16320 tataaatatt atggctgcca ccgttccgcg ttttgacgac gtgtacaaaa atgcacaaag 16380 aagaattcta gatcaagaaa cattttttag tagaggtcta agtagaccgt taatgaaaaa 16440 cacatatcta tttgataatt acgcgtatgg atggatacca gaaactgcaa tttggagtag 16500 tagatacgca aacctagatg ctagtgacta ttatcccatt tcgttgggat tacttaaaaa 16560 gtttgagttt ctcatgtctc tatataaagg tcctattccc gtatatgaag aaaaagtaaa 16620 tactgaattc attgctaatg gatctttctc cggtagatat gtatcatatc ttagaaagtt 16680 ttctgctctt ccaacaaacg agtttattag ttttttgtta ctgacttcca ttccaatcta 16740 taatatcttg ttctggttta aaaatactca gtttgatatt actaaacaca cattattcag 16800 atacgtctat acagataatg ccaaacacct ggcgttggct aggtatatgc atcaaacagg 16860 agactataag cctttgttta gtcgtctcaa agagaattat atatttaccg gtcccgttcc 16920 aataggtatc aaagatatag atcaccctaa tcttagtaga gcaagaagtc catccgatta 16980 tgagacatta gctaatatta gtactatatt gtactttacc aagtatgatc cggtattaat 17040 gtttttattg ttttacgtac ctgggtattc aattactaca aaaattactc cagccgtaga 17100 17160 atgcttcgta aaatgtaggt tttgaaccaa acattctttc aaagaatgag atgcataaaa 17220 ctttattatc caatagattg actatttcgg acgtcaatcg tttaaagtaa acttcgtaaa 17280 atattctttg atcactgccg agtttaaaac ttctatcgat aattgtttca tatgttttaa 17340 tatttacaag ttttttggtc catggtccat taggacaaat atatgcaaaa taatatcgtt 17400 ctccaagttc tatagtctct ggattatttt tattatattc agtaaccaaa tacatattag 17460 ggttatctgc ggatttataa tttgagtgat gcattcgact caacataaat aattctagag 17520 gagacgatct actatcaaat tcggatcgta aatctgtttc taaagaacgg agaatatcta 17580 tacatacctg attagaattc atccgtcctt cagacaacat ctcagacagt ctggttttgt 17640 acatcttaat catattctta tgaaacttgg aaacatctct tctagtttca ctagtacctt 17700 tattaattct ctcaggtaca gattttgaat tcgacgatgc tgagtatttc atcgttgtat 17760 atttcttct cgattgcata atcagattct tatataccgc ctcaaactct attttaaaat 17820 tattaaacaa tactctatta ttaatcagtc gttctaactc tttcgctatt tctatagact 17880 tatcgacatc ttgactgtct atctctgtaa acacggagtc ggtatctcca tacacgctac 17940 gaaaacgaaa tctgtaatct ataggcaacg atgttttcac aatcggatta atatctctat 18000 cgtccatata aaatggatta cttaatggat tggcaaaccg taacataccg ttagataact 18060 <h2 style=";text-align:left;direction:ltr">ctgctccatt tagtaccgat tctagataca agatcattct acgtcctatg gatgtgcaac 18120<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tcttagccga agcgtatgag tatagagcac tatttctaaa tcccatcaga ccatatactg 18180<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> agttggctac tatcttgtac gtatattgca tggaatcata gatggccttt tcagttgaac 18240<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tggtagcctg ttttaacatc tttttatatc tggctctctc tgccaaaaat gttcttaata 18300<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gtctaggaat ggttccttct atcgatctat cgaaaattgc tatttcagag atgaggttcg 18360<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gtagtctagg ttcacaatga accgtaatat atctaggagg tggatatttc tgaagcaata 18420<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gctgattatt tatttcttct tccaatctat tggtactaac aacgacaccg actaatgttt 18480<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> ccggagatag atttccaaag atacacacat taggatacag actgttataa tcaaagatta 18540<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> atacattatt actaaacatt ttttgttttg gagcaaatac cttaccgcct tcataaggaa 18600<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> acttttgttt tgtttctgat ctaactaaga tagttttagt ttccaacaat agctttaaca 18660<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gtggaccctt gatgactgta ctcgctctat attcgaatac catggattga ggaagcacat 18720<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> atgttgacgc acccgcgtct gtttttgttt ctactccata atactcccac aaatactgac 18780 acaaacaagc atcatgaata cagtatctag ccatatctaa agctatgttt agattataat 18840 ccttatacat ctgagctaaa tcaacgtcat cctttccgaa agataattta tatgtatcat 18900 taggtaaagt aggacataat agtacgactt taaatccatt ttcccaaata tctttacgaa 18960 ttactttaca tataatatcc tcatcaacag tcacataatt acctgtggtt aaaacctttg 19020 caaatgcagc ggctttgcct ttcgcgtctg tagtatcgtc accgatgaac gtcatttctc 19080 taactcctct atttaatact ttacccatgc aactgaacgc gttcttggat atagaatcca 19140 atttgtacga atccaatttt tcagattttt gaatgaatga atatagatcg aaaaatatag 19200 ttccattatt gttattaacg tgaaacgtag tattggccat gccgcctact cccttatgac 19260 tagactgatt tctctcataa atacagagat gtacagcttc ctttttgtcc ggagatctaa 19320 agataatctt ctctcctgtt aataactcta gacgattagt aatatatctc agatcaaagt 19380 tatgtccgtt aaaggtaacg acgtagtcga acgttagttc caacaattgt ttagctattc 19440 gtaacaaaac tattcagaa catagaacta gttctcgttc gtaatccatt tccattagtg 19500 actgtatcct caacatcct ctatcgacgg cttctgtat ttcctgttcc gttaacatct 19560 cttcattaat gagcgtaac ataatcgtt taccactta atcgatta cagtaacttg 19620 tatgcgagat tgggttaata atacagaag gaacttctt atcgaagtga cactctatat 19680 ctagaaataa gtacgatctt gggatatcga atctaggtat tttttagcg aacagttac 19740 gtggatcgtc acatgataa catccattgt taatctttgt caatattgc tcgtccaacg 19800 agtaacatcc gtctggagat atcccgttag aaatataaaa ccactaata ttgagaatt 19860 catccatggt ggcattttgt atgctgcgtt tctttggctc ttctcaac cacatatctg 19920 cgacggagca tttctatct ttaatatcta gattataact tattgtctcg tcaatgtcta 19980 tagttctcat ctttcccac ggcctcgcat taatggagg aggagacaat gactgatata 20040 tttcgtccgt aactacgtaa taaaagtaat gaggaatcg tataaataca gtctcaccat 20100 ttcgacatct ggatttcaga tataaaaatc tgtttcacc gtgactttca aaccaattaa 20160 tgcaccgaac atccatttct agaatttaga aatatatttt catttaaatg aatcccaaac 20220 attgggggaag agccgtatgg accattattt ttatagtact ttcgcaagcg ggtttagacg 20280 gcaacataga agcgtgtaaa cgaaaactat atactatagt cagcactctt ccatgtcctg 20340 catgtagacg gcagcgact atcgctataag aggacaataa tgtcatgtct agcgatgatc 20400 tgaattatat ttattatttt ttcatcagat tatttaacaa tttggcatct gatcccaaat 20460 acgcaatcaa tgtgtcaaag gttaaccctt tataaactta acccattata aaacttatga 20520 ttagtcacaa ctgaaataac cgcgtgatta ttttttggta taattctaca cggcatggtt 20580 tctgtgacta tgaattcaac ccccgttaca ttagtgaaat ctttaacaaa cagcaagggt 20640 tcgtcaaaga cataaaactc attgtttaca atcgaaatag accccctatc acacttaaaa 20700 taaaaaatat ccttatcctt taccaccaaa taaaattctg attggtcaat gtgaatgtat 20760 tcacttaaca gttccacaaa tttatttatt aactccgagg cacatacatc gtcggtattt 20820 tttatggcaa actttactct tccagcatcc gtttctaaaa aaatattaac gagttccatt 20880 tatatcatcc aatattattg aaatgacgtt gatggacaga tgatacaaat aagaaggtac 20940 ggtaccttg tccaccatct cctccaattc atgctctatt ttgtcattaa cttaatgta 21000 tgaaaacagt acgccacatg cttccatgac agtgtgtaac actttggata caaaatgttt 21060 gacattagta taattgtcca agactgtcaa tctataatag atagtagcta tatatattc 21120 tatgatggta ttgaagaaga tgacaacctt ggcatattga tcatttaaca cagacatggt 21180 atcaacagat agcttgaatg aaagagaatc agtaattgga ataagcgtct tctcgataga 21240 gtgtccgtat accaacatgt ctgatatttt gatgtattcc attaaattat ttagtttttt 21300 ctttttattt tcgttaaaca gcatttctgt caacggaccc caacatcgtt gaccgattaa 21360 gttttgattg atttttccgt gtaaggcgta tctagtcaga tcgtatagcc tatccaataa 21420 tccatcatct gtgcgtagat cacatcgtac actttttaat tctctataga agagcgacag 21480 acatctggag caattacaga cagcaatttc tttattctct acagatgtaa gatacttgaa 21540 gacattccta tgatgatgca gaattttgga taacacggta ttgatggtat ctgttaccat 21600 aattccttg atggctgata gtgtcagagc acaagatttc caatctttga caatttttag 21660 caccattatc tttgttttga tatctatatc agacagcatg gtgcgtctga caacacaggg 21720 attaagacgg aaagatgaaa tgattctctc aacatcttca atggatacct tgctattttt 21780 tctggcatta tctatatgtg cgagaatatc ctctagagaa tcagtatcct ttttgatgat 21840 agtggatctc aatgacatgg gacgtctaaa ccttcttatt ctatcaccag attgcatggt 21900 gatttgtctt ctttctttta tcataatgta atctctaaat tcatcggcaa attgtctata 21960 tctaaaatca tatatgaga tgtttacctc tacaaatatc tgttcgtcca atgttagagt 22020 atctacatca gttttgtatt ccaaattaaa catggcaacg gatttaattt tatattcctc 22080 tattaagtcc tcgtcgataa taacagaatg tagataatca tttaatccat cgtacatggt 22140 tggaagatgc ttgttgacaa aatctttaat tgtcttgatg aaggtgggac tatatctaac 22200 atcttgatta ataaaattta taacattgtc cataggatac tttgtaacta gttttataca 22260 catctcttca tcggtaagtt tagacagaat atcgtgaaca ggtggtatat tatattcatc 22320 agatatacga agaacaatgt ccaaatctat attgtttaat atattatata gatgtagcgt 22380 agctcctaca ggaatatctt taactaagtc aatgatttca tcaaccgtta gatctatttt 22440 aaagttaatc atataggcat tgatttttaa aaggtatgta gccttgacta cattttcatt 22500 aattaaccat tccaagtcac tgtgtgtaag aagattatat tctatcataa gcttgactac 22560 atttggtcc aataccatta aagaattctt atgatataag gaaacagctt ttaggtactc 22620 atctactcta caagaatttt ggagagcctt aacgatatca gtgacgttta ttatttcagg 22680 aggaaagaat ctaacattga gaatatcgga attaatagct tccagataca gtgattttgg 22740 caatagtccg tgtaatccat aatccagtaa cacgagctgg tgcttgctag acaccttttc 22800 aatgtttaat ttttttgaaa tagctttga taaagccttc ctcgcaaatt ccggatacat 22860 gaacatgtcg gcgacatgat taagtattgt ttttcatta ttttctcaac aagttctcaa 22920 taccccaata gatgatagaa tatcacccaa tgcgtccatg ttgtctattt ccaacaggtc 22980 gctatatcca ccaatagaag ttttcccaaa aaagattcta ggaacagttc taccaccagt 23040 aatttgttca aaataatccc gcaattcatt ttcgggttta aattctttaa tatcgacaat 23100 ttcatacgct cctcttttga aactaaactt atttagaata tccagtgcat ttctacaaaa 23160 aggacatgta tacttgacaa aaattgtcac tttgttattg gccaaccttt gttgtacaaa 23220 ttcctcggcc attttaatat ttaagtgata taaaactatc tcgacttatt taactcttta 23280 gtcgagatat atggacgcag atagctatat gatagtaggg ataacagggt aat 23333 <210> 4 <211> 26445 <212> DNA <213> Vaccinia virus <400> 4 tagggataac agggtaattt tctagaattt agaaatatat tttcatttaa atgaatccca 60 aacattgggg aagagccgta tggaccatta tttttatagt actttcgcaa gcgggtttag 120 acggcaacat agaagcgtgt aaacgaaaac tatatactat agtcagcact cttccatgtc 180 ctgcatgtag acggcacgcg actatcgcta tagaggacaa taatgtcatg tctagcgatg 240 atctgaatta tatttattat tttttcatca gattatttaa caatttggca tctgatccca 300 aatacgcaat caatgtgtca aaggttaacc ctttataaac ttaacccatt ataaaactta 360 tgattagtca caactgaaat aaccgcgtga ttattttttg gtataattct acacggcatg 420 gtttctgtga ctatgaattc aacccccgtt acattagtga aatctttaac aaacagcaag 480 ggttcgtcaa agacataaaa ctcattgttt acaatcgaaa tagaccccct atcacactta 540 aaataaaaaa tatccttatc ctttaccacc aaataaaatt ctgattggtc aatgtgaatg 600 tattcactta acagttccac aaatttattt attaactccg aggcacatac atcgtcggta 660 ttttttatgg caaactttac tcttccagca tccgtttcta aaaaaatatt aacgagttcc 720 atttatatca tccaatatta ttgaaatgac gttgatggac agatgataca aataagaagg 780 tacggtacct ttgtccacca tctcctccaa ttcatgctct attttgtcat taactttaat 840 gtatgaaaac agtacgccac atgcttccat gacagtgtgt aacactttgg atacaaaatg 900 tttgacatta gtataattgt ccaagactgt caatctataa tagatagtag ctataatata 960 ttctatgatg gtattgaaga agatgacaac cttggcatat tgatcattta acacagacat 1020 ggtatcaaca gatagcttga atgaaagaga atcagtaatt ggaataagcg tcttcgat 1080 agagtgtccg tataccaaca tgtctgatat tttgatgtat tccattaaat tatttagttt 1140 tttcttttta tttcgttaa acagcatttc tgtcaacgga ccccaacatc gttgaccgat 1200 taagttttga ttgatttttc cgtgtaaggc gtatctagtc agatcgtata gcctatccaa 1260 taatccatca tctgtgcgta gatcacatcg tacacttttt aattctctat agaagagcga 1320 cagacatctg gagcaattac agacagcaat ttctttattc tctacagatg taagatactt 1380 gaagacattc ctatgatgat gcagaattt ggataacacg gtattgatgg tatctgttac 1440 cataattcct ttgatggctg atagtgtcag agcacaagat ttccaatctt tgacaatttt 1500 tagcaccatt atctttgttt tgatatctat atcagacagc atggtgcgtc tgacaacaca 1560 gggattaaga cggaaagatg aaatgattct ctcaacatct tcaatggata ccttgctatt 1620 ttttctggca ttctatat gtgcgagaat atcctctaga gaatcagtat cctttttgat 1680 gatagtggat ctcaatgaca tgggacgtct aaaccttctt attctatcac cagattgcat 1740 ggtgatttgt cttctttcttt ttcataat gtaatctcta aattcatcgg caaattgtct 1800 atatctaaaa tcataatatg agatgtttac ctctacaaat atctgttcgt ccaatgttag 1860 agtatctaca tcagttttgt attccaaatt aaacatggca acggatttaa ttttatattc 1920 ctctattaag tcctcgtcga tataacaga atgtagataa tcatttaatc catcgtacat 1980 ggttggaaga tgcttgttga caaaatcttt aattgtcttg atgaaggtgg gactatatct 2040 aacatcttga ttaataaaat ttataacatt gtccatagga tactttgtaa ctagttttat 2100 acacatctct tcatcggtaa gtttagacag aatatcgtga acaggtggta tattatattc 2160 atcagatata cgaagaacaa tgtccaaatc tatattgttt aatatattat atagatgtag 2220 cgtagctcct acaggaatat ctttaactaa gtcaatgatt tcatcaaccg ttagatctat 2280 tttaaagtta atcatatagg cattgattttt taaaaggtat gtagccttga ctacatttc 2340 attaattaac cattccaagt cactgtgtgt aagaagatta tattctatca taagcttgac 2400 tacatttggt cccaatacca ttaaagaatt cttatgatat aaggaaacag cttttaggta 2460 ctcatctact ctacaagaat tttggagagc cttaacgata tcagtgacgt ttattattc 2520 aggaggaaag aatctaacat tgagaatatc ggaattaata gcttccagat acagtgattt 2580 tggcaatagt ccgtgtaatc cataatccag taacacgagc tggtgcttgc tagacacctt 2640 ttcaatgttt aattttttg aaataagctt tgataaagcc ttcctcgcaa attccggata 2700 catgaacatg tcggcgacat gattaagtat tgttttttca ttattttctc aacaagttct 2760 caatacccca atagatgata gaatatcacc caatgcgtcc atgttgcta tttccaacag 2820 gtcgctatat ccaccaatag aagttttccc aaaaaagatt ctaggaacag ttctaccacc 2880 agtaatttgt tcaaaataat cccgcaattc atttcgggt ttaaattctt tatatcgac 2940 aatttcatac gctcctcttt tgaaactaaa cttatttaga atatccagtg catttctaca 3000 aaaaggacat gtatacttga caaaaattgt cactttgtta ttggccaacc tttgttgtac 3060 aaattcctcg gccattttaa tatttaagtg atataaaact atctcgactt atttaactct 3120 ttagtcgaga tatatggacg cagatagcta tatgatagcc aactacagaa ggcaaacgct 3180 ataaaaaaca taattacgac gagcatattt ataaatattt ttattcagca ttacttgata 3240 tagtaatatt aggcacagtc aaacattcaa ccactctcga tacattaact ctctcatttt 3300 cttaacaaa ttctgcaata tcttcgtaaa aagattcttg aaacttttta gaatatctat 3360 cgactctaga tgaatagcg ttcgtcaaca tactatgttt tgtatacata aaggcgccca 3420 ttttaacagt ttctagtgac aaaatgctag cgatcctagg atcctttaga atcacataga 3480 ttgacgattc gtctctctta gtaactctag taaaatatc atacaatcta gtacgcgaaa 3540 tatattatc cttgacttga ggagatctaa acaatctagt tttgagaaca tcgataagtt 3600 catcgggaat gacatacata ctatctttaa tagaactctt ttcatccagt tgaatggatt 3660 cgtccttaac caactgatta atgagatctt ctattttatc atttccaga tgatatgtat 3720 gtccattaaa gttaaattgt gtagcgcttc ttttagtct agcagccaat actttaacat 3780 cactaatatc gatatacaaa ggagatgatt tatcgatggc attagaatt cgttttttcga 3840 catctgtcaa aaccaatttcc ttttgctg tatcatccag ttttccattcc tttgtaaaga 3900 aattattttc tactagacta ttaatagac tgataggat tcctccataa ttgcacaatc 3960 haaactttt cacaaacta gactttacaa gatctacagg atgcgtact tcaggttttct 4020 tagcttgtga ttttcttt tgtgacatt tcttgtgac caaccatct accattcat 4080 tgattttagc agtgaaataa gctttcaatg cacgggcact gattattg aaacgagtt 4140 gatctcaa ttccgccatt taagttcacc aaaacactt taaatacaa tatatcaata 4200 gtagtagaat aagactata aaaaaaata taattacca ataccaccc CACAACgg 4260 tattattagt tgatgtgta gttttctcat cacttagaac agatttaca atttctataa 4320 agtctgtcaa atcatctttc ggagaaccca tatatacacc aaatagcg gcgtacaact 4380 tatccattta tacattgaat attggtttt ctttatcgct atctcatca tattcatcat 4440 caatcaac aagtcccaga ttacgagcca gatctctctc tacattttca gtcattgata 4500 cacgttcact atctccagag agtccgataa cgttagccac cacttctcta tcaatgatta 4560 gtttcttgag tgcgaatgta attttgttt ccgttccgga tctatagaaa actacaggtg 4620 tgataattgc cttggccaat tgtctttctc ttttactgag tgattctagt tcaccttcta 4680 tagatctgag aatggatgat tctccagtcg aaacatattc taccatggat ccgtttaatt 4740 tgttgatgaa gatggattca tccttaaatg ttttctctgt aatagtttcc accgaaagac 4800 tatgcaaaga atttggaatg cgttccttgt gcttaatgtt tccatagacg gcttctagaa 4860 gttgatacaa cataggacta gccgcggtaa cttttatttt tagaaagtat ccatcgcttc 4920 tatcttgttt agatttattt ttaataaagtt tagtctctcc ttccaacata ataaaagtgg 4980 aagtcatttg actagataaa ctatcagtaa gttttataga gatagacgaa caattagcgt 5040 attgagaagc atttagtgta acgtattcga tacattttgc attagattta ctaatcgatt 5100 ttgcatactc tataacaccc gcacaagtct gtagagaatc gctagatgca gtaggtcttg 5160 gtgaagtttc aactctcttc ttgattacct tactcatgat taaacctaaa taattgtact 5220 ttgtaatata atgatatata ttttcacttt atctcatttg agaataaaaa tgtttttgtt 5280 taaccactgc atgatgtaca gatttcggaa tcgcaaacca ccagtggttt tattttatcc 5340 ttgtccaatg tgaattgaat gggagcggat gcgggtttcg tacgtagata gtacattccc 5400 gtttttagac cgagactcca tccgtaaaaa tgcatactcg ttagtttgga ataactcgga 5460 tctgctatat ggatattcat agattgactt tgatcgatga aggctcccct gtctgcagcc 5520 atttttatga tcgtcttttg tggaatttcc caaatagttt tataaactcg cttaatatct 5580 tctggaaggt ttgtattctg aatggatcca ccatctgcca taatcctatt cttgatctca 5640 tcattccata attttctctc ggttaaaact ctaaggagat gcggattaac tacttgaaat 5700 tctccagaca atactctccg agtgtaaata ttactggtat acggttccac cgactcatta 5760 tttcccaaaa tttgagcagt tgatgcagtc ggcataggtg ccaccaataa actatttcta 5820 agaccgtatg ttctgatttt atcttttaga ggttcccaat tccaaagatc cgacggtaca 5880 acattccaaa gatcatattg tagaataccg ttactggcgt acgatcctac atatgtatcg 5940 tatggtcctt ccttctcagc tagttcacaa ctcgcctcta atgcaccgta ataaatggtt 6000 tcgaagatct tcttatttag atcttgtgct tccaggctat caaatggata atttaagaga 6060 ataaacgcgt ccgctaatcc ttgaacacca ataccgatag gtctatgtct cttattagag 6120 atttcagctt ctggaatagg ataataatta atatctataa ttttattgag atttctgaca 6180 attactttga ccacatcctt cagtttgaga aaatcaaatc gcccatctat tacaaacatg 6240 ttcaaggcaa cagatgccag attacaaacg gctacctcat tagcatccgc atattgtatt 6300 atctcagtgc aaagattact acacttgata gttcctaaat tttgttgatt actctttttg 6360 ttacacgcat ccttataaag aatgaatgga gtaccagttt caatctgaga ttctataatc 6420 gctttccaga cgactcgagc ctttattata gatttgtatc tcctttctct ttcgtatagt 6480 gtatacaatc gttcgaactc gtctccccaa acattgtcca atccaggaca ttcatccgga 6540 cacatcaacg accactctcc gtcatccttc actcgtttca taaagagatc aggaatccaa 6600 agagctataa atagatctct ggttctatgt tcctcgtttc ctgtattctt tttaagatcg 6660 aggaacgcca tatatcaga atgccacggt tccaagtata tggccataac tccaggccgt 6720 ttgtttcctc cctgatctat gtatctagcg gtgttattat aaactctcaa cattggaata 6780 ataccgtttg atataccatt ggtaccggag atatagcttc cactggcacg aatattacta 6840 attgatagac ctattccccc tgccatttta gagattaatg cgcatcgttt taacgtgtca 6900 tagataccct ctatgctatc atcgatcatg ttaagtagaa aacagctaga catttggtga 6960 cgactagttc ccgcattaaa taaggtagga gaagcgtgcg taaaccattt ttcagaaagt 7020 agattgtacg tctcaatagc tgagtctata tcccattgat gaattcctac tgcgacacgc 7080 attaacatgt gctgaggtct ttcaacgatc ttgttgttta ttttcaacaa gtaggatttt 7140 tccaaagttt taaaaccaaa atagttgtat gaaaagtctc gttcgtaaat aataaccgag 7200 ttgagttat ccttatattt gttaactata tccatggtga tacttgaaat aatcggagaa 7260 tgtttcccat ttttaggatt aacatagttg aataaatcct ccatcacttc actaaatagt 7320 ttttttgttt ccttgtgtag atttgatacg gctattctgg cggctagaat ggcataatcc 7380 <h2 style=";text-align:left;direction:ltr">ggatgttgtg tagtacaagt ggctgctatt tcggctgcca gagtgtccaa ttctaccgtt 7440<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gttactccat tatatattcc ttgaataacc ttcatagcta ttttaatagg atctatatga 7500<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tccgtgttta agccataaca taattttcta atacgagacg tgattttatc aaacatgaca 7560<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> ttttccttgt atccatttcg tttaatgaca aacatttttg ttggtgtaat aaaaaaatta 7620<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tttaactttt cattaatagg gatttgacgt atgtagcgta caaaattatc gttcctggta 7680<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tatagataaa gagtcctata tatttgaaaa tcgttacggc tcgattaaac tttaatgatt 7740<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> gcatagtgaa tatatcatta ggattttaact ccttgactat catggcggcg ccagaaatta 7800<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> ccatcaaaag cattaataca gttatgccga tcgcagttag aacggttata gcatccacca 7860<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tttatatcta aaaattagat caaagaatat gtgacaaagt cctagttgta tactgagaat 7920<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tgacgaaaca atgtttctta catatttttt tcttattagt aaccgactta atagtaggaa 7980<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> ctggaaaact agacttgatt attctataag tatagatacc cttccaaata atattctctt 8040<h2 style=";text-align:left;direction:ltr"> <h2 style=";text-align:left;direction:ltr"> tgataaaagt tccagaaaat gtagaatttt ttaaaaagtt atcttttgct attaccaaga 8100<h2 style=";text-align:left;direction:ltr"> ttgtgtttag acgcttatta ttaatatgag tgatgaatc cacaccgcct ctagatatcg 8160 cttttattc cacattagat ggtaaatcca atagtgaac tatcttttta ggaatgtatg 8220 gactcgcgtt tagaggattg aacgtcttgg gcgtcggaaa ggatgatcg tcaacgaat 8280 aaacaatttc acaatggat gttaatgtat tagtaggaa tttttgacg ctagtggaat 8340 tgaagattct atgatgat gttctaccta ttcatccga tacatgtta atttccgaca 8400 ccaacggttt taatattcg atgatatacg gtagtctctc tttcggactt atatagctta 8460 ttccacaata cgagtcatta tatactccaa aaaacaaat aactagtata aaatctgtat 8520 cgaatgggaa aaacgaaatt atcgacatag gtatagaatc cggaacattg aacgtattaa 8580 tacttaattc ttttctgtg gtaagtaccg ataggttatt gatatgtat ggttttaaat 8640 attctataac ttgagacttg atagatatta gtgatgaatt gaaaattatt tttatcacca 8700 cgtgtgtttc aggatcatcg tcgacgcccg tcaaccacc gatggagta aataaataat 8760 cattaatata tgctctaaat attagtattt ttattaatcc ttgattatc atcttctcgt 8820 acgcgaatga ttccatgatc aagagtgatt tgagaacatc ctccggagta ttaatgggct 8880 tagtaaacag tacatcgttg caataataa agttatccaa gttaaaggat attatgcatt 8940 cgtttaaaga tatcacctca tctgacggag acaatttttt ggtaggtttt agagactttg 9000 aagctacttg tttaacaaag ttatcatcg tcgttacta ttctatttaa ttttgtagtt 9060 aatttatcac atatcacatt aattgacttt ttggtccact tttccatacg tttatattct 9120 tttaatcctg cgttatccgt ttccgttata tccagtgata gatcgtgcag gttaaataga 9180 atgctcttaa ataatgtcat tttcttatcc gctaaaaatt taaagaatgt ataaaccttt 9240 ttcagagatt tgaaactctt aggtggtgtc ctagtacaca atatcataaa caaactataa 9300 aacattccac attcagattc caacagctga ttaacttcta cattaataca gcctattttc 9360 gctccaaatg tacattcgaa aaatctgaat aaaacatcga tgtcacaatt tgtattatcc 9420 aatacagaat gtctgtgatt cgtgttaaaa ccatcggaga aggaatagaa ataaaaatta 9480 ttatagtggt ggaattcagt tggaatattg cctccggagt cataaaagga tactaaacat 9540 tgttttttat cataaattac acatttccaa tgagacaaat aacaaaatcc aaacattaca 9600 aatctagagg tagaactttt aattttgtct ttaagtatat acgataagat atgtttattc 9660 ataaacgt caaatttttc atgaatcgct aaggagttta agaatctcat gtcaaattgt 9720 cctatataat ccacttcgga tccataagca aactgagaga ctaagttctt aatacttcga 9780 ttgctcatcc aggctcctct ctcaggctct atttcatct tgacgacctt tggattttca 9840 ccagtatgta ttcctttacg tgataaatca tcgattttca aatccatttg tgagaagtct 9900 atcgccttag atactttttc ccgtagtcga ggtttaaaga aatacgctaa cggtatacta 9960 gtaggtaact caaagacatc atatagaa tggtaacgcg tctttaactc gtcggttaac 10020 tctttcttt gatcgagtc gtcgctacta ttgggtctgc tcaggtgccc cgactctact 10080 agttccaaca tcataccgat aggaatacaa gacactttgc cggcggttgt agatttatca 10140 tatttttcca ctacatatcc gttacaattt gttaaaaatt tagatacatc tatattgcta 10200 cataatccag ctagtgaata tatatgacat aataaattgg taaatcctag ttctggtatt 10260 ttactaatta ctaaatctgt atatctttcc atttatcatg gaaagaatt taccagatat 10320 cttctttttt ccaactgcg ttaatgtatt ctcttacaaa tattcacag atgaattcag 10380 taatatgagt aaacggaac gtgatagtttt ctcattggcc gtgttttccag ttataaaaca 10440 taggatggcat aacgcacacg ttgtaaaca aaggaata tacaagtta gtacagaagc 10500 acgtggaaaa aaagtatctc ctccatcact aggaaaccc gcacacataa acctaccac 10560 gaaacaat atacagtg aacacacaat aagctttgaa tgttatagtt ttctaaaatg 10620 tataacaat acagaatca attcgttcga tgagtatata ttagaggac tattagaagc 10680 tggtaatagt ttacagatat tttccaattc cgtaggtaaa cgaacagata ctataggtgt 10740 actagggaat aagtatccat ttagcaaat tccattggcc tcattaacc ctaaagcaca 10800 acgagagata ttttagcgt ggatttctca tagactgta gttttaactg gaggaactgg 10860 agtgggtaag acgtcacagg tacccagtt attgctttgg tttattatt tattttggtgg 10920 attctctact ctagataaaa tcactgactt tcacgaaaga cagtcattc tatctcttcc 10980 tagtagct tagttagat tgcatagcaa taccatttta aaatcattgg gatttaaggt 11040 actagatgga tctcctattt ctttacggta cggatctata ccggaagaat tataaacaa 11100 acaaccaaaa aaatatggaa ttgtattttc tacccataag ttatctctaa caaaactatt 11160 tagttatggc actcttatta tagacgaagt tcatgagcat gatcaaatag gagatattat 11220 tatagcagta gcgagaaagc atcatacgaa aatagattct atgtttttaa tgactgccac 11280 gttagaggat gacagggaac ggctaaaagt atttttacct aatccccgcat ttatacatat 11340 tcctggagat acactgttta aaattagcga ggtatttatt cataataaga taaatccatc 11400 ttccagaatg gcatacatag aagaagaaaa gagaaattta gttactgcta tacagatgta 11460 tactcctcct gatggatcat ccggtatagt cttttgtggca tccgttgcac agtgtcacga 11520 atataaatca tatttagaaa aaagattacc gtatgatatg tatattattc atggtaaggt 11580 tttagatata gacgaaatat tagaaaaagt gtattcatca cctaatgtat cgataattat 11640 ttctactcct tatttggaat ccagcgttac tatacacaat gttacacaca tttatgatat 11700 gggtagagtt tttgtccccg ctccttttgg aggatcgcaa caatttattt ctaaatctat 11760 gagagatcaa cgaaaaggaa gagtaggaag agttaatcct ggtacatacg tctatttcta 11820 tgatctgtct tatatgaagt ctatacagcg aatagattca gaatttctac ataattatat 11880 attgtacgct aataagttta atctaacact ccccgaagat ttgtttataa tccctacaaa 11940 tttggatatt ctatggcgta caaaggaata tatagactcg ttcgatatta gtacagaaac 12000 atggaataaa ttattatcca attattatat gaagatgata gagtatgcta aactttatgt 12060 actaagtcct attctcgctg aggagttgga taattttgag aggacgggag aattaactag 12120 tattgtacaa gaagccattt tatctctaaa tttacgaatt aagattttaa atttaaaca 12180 taaagatgat gatacgtata tacactttg taaaatatta ttcggtgtct ataacggaac 12240 aaacgctact atatattatc atagacctct aacgggatat atgaatatga tttcagatac 12300 tatatttgtt cctgtagata ataactaaaa attaaactct aatgaccaca tcttttttta 12360 gagatgaaaa attttccaca tctccttttg tagacacgac taaacatttt gcagaaaaaa 12420 gtttattagt gtttagataa tcgtatactt catcagtgta gatagtaaat gtgaacagat 12480 aaaaggtatt cttgctcaat agattggtaa attccataga atatattaat cctttcttct 12540 tgagatccca catcatttca accagagacg ttttatccaa tgatttacct cgtactatac 12600 cacatacaaa actagatttt gcagtgacgt cgtacctggt attcctacca aacaaaattt 12660 tacttttagt tcttttagaa aattctaagg tagaatctct atttgccaat atgtcatcta 12720 tggaattacc actagcaaaa aatgatagaa atatatattg atacatcgca gctggttttg 12780 atctactata ctttaaaaac gaatcagatt ccataattgc ctgtatatca tcagctgaaa 12840 aactatgttt tacacgtatt ccttcggcat ttctttttaa tgatatatct tgtttagaca 12900 atgataaagt tatcatgtcc atgagagacg cgtctccgta tcgtataaat atttcattag 12960 atgttagacg cttcattagg ggtatacttc tataaggttt cttaatcagt ccatcattgg 13020 ttgcgtcaag aactactatc ggatgttgtt gggtatctct agtgttacac atggccttac 13140. 13140. 13140. 13140. 13140. 13140. 13140. atttcatttg tcaaggatat tagtatcgt ttgctatcgt cattaatacg tgtaatgtaa tcatataaat catgcgatag ccaaggaaaa tttaaataga tgttcatcat ataatcgtcg ctaattca father ttgacattga ctaatttgta fathercctc gccacgaaga aagctctcgt attcagtttc atcgataaag gataccgtta fatheractg gttgccgata gtctcatagt ctattaagtg gtaagtttcg tacaaataca gaatccctaa aatattatct aatgttggat 13500. 13500. 13500. 13500. 13500. 13500. 13500. 13500 ttactggaat agatgaagga aatctccg gacatgctgg taaagaccca aatgtctgtt tgaagaaatc caatgttcca ggtcctaatc tcttaacaaa aattacgata ttcgatcccg atatcctttg cattctattt accagcatat cacgaactat attack tctatcatgt ctattctccc accgttat aaatcgcctc cgctaagaaa cgttagtata tccatacaat ggaatacttc atttctaaaa tagtattcgt tttctaattc tttaatgtga aatcgtatac 13800 tagaaaggga aaaattatct ttgagttttc cgttagaaaa gaaccacgaa actaatgttc 13860 tgattgcgtc cgattccgtt gctgaattaa tggatttaca ccaaaaactc atataacttc 13920 tagatgtaga agcattcgct aaaaaattag tagaatcaaa ggatataagt agatgttcca 13980 acaagtgagc aattcccaag atttcatcta tatcattctc gaatccgaaa ttagaaattc 14040 ccaagtagat atccttttc atccgatcgt tgatgaaaat acgaacttta ttcggtaaga 14100 caatcattta ctaaggagta aaataggaag taatgttcgt atgtcgttat catcgtataa 14160 attaaaggtg tgttttttac cattaagtga cattataatt ttaccaatat tggaattata 14220 atataggtgt atttgcgcac tcgcgacggt tgatgcatcg gtaaatatag ctgtatctaa 14280 tgttctagtc ggtatttcat cattcgctg tctaataata gcgttttctc tatctgtttc 14340 cattacagct gcctgaagtt tattggtcgg ataatatgta aaataataag aaatacatac 14400 gaataaaaaaaaata gatataata agatgccatt tagagatcta atttgttca 14460 acttgtccaa attcctactt acagagatg aggaatcgtt ggagatagtg tcttccttat 14520 gtagaggatt tgaaatatct tatgatgact tgataactta ctttccagat aggaatacc 14580 fatherataatttaagta ttgaacatg tagatttc ggaggatta agtaggat 14640 tccatgatac aactctgaga gatttagtct atcttagatt gtacaagtat tccaagtgta 14700 tacggccgtg ttaatta ggagataatc taaaaggcat agttgtta aaggacagga 14760 atttattat taggagca atgatgact tgatagata tctcctcag gattacactc 14820 ctcagattta tacatattct atgagcgag ttcccatagc tggttcaaaa ttaattcttt 14880 gtggattttc tcaagttaca ttatggcgt atacaacgtc gcatataaca acaataaaa 14940 aggtagatgt tctcgtttcc aaaaaatgta tagatgact agtcgatcca ataaattatc 15000 aaatacttca aaatttattt gataaggaa gcggaacat aaaaaata ctcaggaga 15060 tattttattc ggtacaggt ggccaactc cataggtagc ttttctatt tcggatttta 15120 gaatttccaa attcaccagc gatttatcgg ttttggtgaa atccaaggat ttattaatgt 15180 ccacaaatgc catttgtttt gtctgtggat tgtatttgaa aatggaaacg atgtagttag 15240 atagatgcgc tgcaaagttt cctattaggg ttccgcgctt tacgtcaccc agcatacttg 15300 aatcaccatc ctttaaaaaa aatgataaga tatcaacatg gagtatatca tactcggatt 15360 ttaattcttc tactgactca ctgacatttt cacaaatact acaatacggt ttaccgaaaa 15420 taatcaatac gttcttcatt tatgggtatc aaaaacttaa aatcgttact gctggaaaat 15480 aaatcactga cgatattaga tgataattta tacaaagtat acaatggaat atttgtggat 15540 acaatgagta tttatatagc cgtcgccaat tgtgtcagaa acttagaaga gttaactacg 15600 gtattcataa aatacgtaaa cggatgggta aaaaagggag ggcatgtaac cctttttatc 15660 gatagaggaa gtataaaaat taaacaagac gttagagaca agagacgtaa atattctaaa 15720 ttaaccaagg acagaaaaat gttagaatta gaaaagtgta catccgaaat acaaaatgtt 15780 accggattta tggaagaaga aataaaggca aaaatgcaat taaaaatcga taaacttaca 15840 tttcaaatat atttatctga ttctgataac ataaaaatat cattgaatga gatactaaca 15900 catttcaaca ataatgagaa tgttacatta ttttatgtg atgaacgaga cgcagaattc 15960 gttatgtgtc tcgaggctaa aacacatttc tctaccacag gagaatggcc gttgataata 16020 agtaccgatc agtaactat gctatttgca tctgctgata atcatcctaa gatgataaaa 16080 aacttaactc aactgtttaa atatgttcca tctgcagagg ataactattt agcaaaatta 16140 acggcgttag tgaatggatg tgatttcttt cctggactct atggggcatc tataacaccc 16200 aacaacttaa acaaaataca attgtttagt gattttacaa tcgataatat agtcactagt 16260 ttggcaatta aaaattatta tagaaagact aactctaccg tagacgtgcg taatattgtt 16320 acgtttataa acgattacgc taatttagac gatgtctact cgtatgttcc tccttgtcaa 16380 tgcactgttc aagaatttat attttccgca ttagatgaaa aatggaacaa ttttaaatca 16440 tcttatttag aaaccgttcc gttaccctgt caattaatgt acgcgttaga accacgtaag 16500 gagattgatg tttcagagt taaaacttta tcatcttata tagattcga aaatactaaa 16560 tcagatatcg atgttataaa atctatatcc tcgatttg gatattctaa cgaaaactgt 16620 aacactatag tgttcggcat ctataaggat atttactac tgagtataaa tagttcattt 16680 tactttaacg atagtctgtt aaaccaat actaaaagtg atatataat aatataggt 16740 tactagatta aaaatggtgt tccaacct gtgctctaca tgcggtaag atatttctca 16800 cgaacgatat aattgatta tacgaaaaaa atcattaaag gatgtactcg tcagtgtaaa 16860 gaacgaatgt tgtaggttaa attackacac acatagaa cctcaacgta acttacagt 16920 gcaacctcta ttggatataa actaatatgg atccggttaa ttttatcaag acatagcgc 16980 ctagaggttc tattatttt attaattata ccatgtcatt aacaagtcat ttgaatccat 17040 cgatagaaaa acatgtgggt atttattatg gtacgttatt atcggaacac ttggtagttg 17100 aatctaccta taggaaagga gttcgaatag tcccattgga tagtttttt gaaggatatc 17160 ttagtgcaaa agtatacatg ttagagaata ttcaagttat gaaatagca gctaatacgt 17220 cattaacttt attgggtatt ccgtatggat ttggtcataa tagaatgtat tgttttaaat 17280 tggtagctga atgtataaa aatgccggta ttgatacatc gtctaaacga atattgggca 17340 aagatatttt tctgagccaa aacttcacag acgataatag atggataaag atatatgatt 17400 ctaataattt aacattttgg caaattgatt accttaaagg gtgagttaat atgcataact 17460 actcctccgt tgttttttcc ctcgttcttt ttcttaacgt tgtttgccat cactctcata 17520 atgtaaagat attctaaaat ggtaaacttt tgcatatcgg acgcagaaat tggtataaat 17580 gttgtaattg tattattcc cgtcaatgga ctagtcacag ctccatcagt tttatcct 17640 ttagagtatt tctcactcgt gtctaacatt ctagagcatt ccatgatctg tttatcgttg 17700 atattggccg gaaagataga ttttttatt tttattatat tactattggc aattgtagat 17760 ataacttctg gtaaatattt ttctaccttt tcaatctctt ctattttcaa gccggctata 17820 tattctgcta tattgttgct agtatcaata ccttttcttg ctaagaagtc atatgtggta 17880 ttcactatat cagttttaac tggtagttcc attagcctttt ccacttctgc agaatca gaattggtt ctttaccaga aaatccagct actatatag gctcaccgat gatcattggc aaaatcctat attgtaccag attatgaga gcatatttca tttccaata ttctgctagt tcttgagaca ttgatttatt tgatgaatct agttggttct ctagatactc taccatttct gccgcataca ataacttgtt agtaaaatc agggttatca aagtgtttag cgtggctaga atagtgggct tgcatgtatt aaagaatgcg gtagtatgag taaccgttt taacgaatta tatagtctcc aagaatctgt ggcgttacat acatgagccg aatgacatcg aagattgtcc aatatttta atagctgctc tttgtccatt atttctatat ttgactcgca acaattgtaa attack tcactgattc ctttttcgat gccggacaat agcacaattg tttagctttg gactctatgt attcagaatt father tctcttaata cagattgcac fathercatttt gaaactatgt caaaaattgt agaacgacgc tgttctgcag ccatttaact ttaaataatt tacaaaaatt taaaatgagc atccgtataa aaatcgataa actgcgccaa attgtggcat 18600 atttttcaga gttcagtgaa gaagtgtcta taaatgtaga ctcgacggat gagttaatgt 18660 atatttttgc cgccttgggc ggatctgtaa acatttgggc cattatacct ctcagtgcat 18720 cagtgttcta ccgcggagcc gaaaatattg tgtttaatct tccggtgtcc aaggtaaaat 18780 cgtgtttgtg tagttttcac aatgatgcca tcatagatat agaacctgat ctgaaaaata 18840 atctagtaaa actttctagt tatcatgtag taagtgtcga ttgtaacaag gaactgatgc 18900 ctattaggac agatactact atttgtctaa gtatagatca aaagaaatct tatgtgttta 18960 atttcacaa gtatgaagaa aaatgttgtg gtagaaccgt cattcatcta gaatggttgt 19020 tgggcttat caagtgtatt agtcagcatc agcatctggc tattatgttt aaagatgaca 19080 atattattat gaagactcct ggtaatactg atgcattttc cagggaatat tctatgactg 19140 aatgttctca agaactacaa aagttttctt tcaaatagc tatctcgtct ctcaacaaac 19200 tacgaggatt caaaaagaga gtcaatgttt ttgaaactag aatcgtaatg gataatgacg 19260 aacatttt aggaatgttg ttttcggata gagttcaatc ctttagatc aacatcttta 19320 tggcgtttt agattaatac ttcaatgag aataatatgg gtggcggagt aagtgttgag 19380 ctccctaaac gggatccgcc tccgggagta cccactgatg agatgttatt aaacgtggat 19440 aaaatgcatg acgtgatagc tcccgctaag ctttagaat atgtgcatat aggaccacta 19500 gcaaagata agaggaata agtaagaaa agaatccag agtttagat agtcacaca 19560 ggacccggtg gtctttcggc attgttaaga caatcgtata atggaccgc accaattgc 19620 tgtcgcactt ttaatcgtac tcattattgg aaaaggatg gaagatatc agatagtat 19680 gaagaggggtg cagtattaga atcgtgttgg ccagacgttc acgacactgg aaatgcgat 19740 gttgatttat tcgactggtg tcagggggat acgttcgata gaacatatg ccatcagtgg 19800 atcggttcag cctttaatag gagtgataga actgtagagg gtcaacaatc gttaataaat 19860 ctgtataata agatgcaaac attatgtagt aagatgcta gtgtaccaat atgtgaatca 19920 tttttgcatc atttacgcgc acacaataca gaagatagca aagagatgat cgattatatt 19980 ctaagacaac agtctgcgga ctttaaacag aaatatatga gatgtagtta tcccactaga 20040 gataagttag aagagtcatt aaaatatgcg gaacctcgag aatgttggga tccagagtgt 20100 tcgaatgcca atgttaattt cttgctaaca cgtaattata ataatttagg actttgcaat 20160 attgtacgat gtaataccag cgtgaacaac ttacagatgg ataaaacttc ctcattaaga 20220 ttgtcatgtg gattaagcaa tagtgataga ttttctactg ttccccgtcaa tagagcaaaa 20280 gtagttcaac ataatattaa acattcgttc gacctaaaat tgcatttgat cagtttatta 20340 tctctcttgg tatatggat actaattgta gctatttaaa tgggtgccgc ggcaagcata 20400 cagacgacgg tgaatacact cagcgaacgt atctcgcta aattagaaca agaagcgaac 20460 gctagtgctc aaacaaaatg tgatatagaa atcggaaatt tttatccg acaaaaccat 20520 ggatgtaacc tcactgttaa aaatatgtgc tctgcggacg cggatgctca gttggatgct 20580 gtgttatcag ccgctacaga aacatatagt ggattaacac cggaacaaaa agcatacgtg 20640 ccagctatgt ttactgctgc gttaaacatt cagacgagtg taacactgt tgttagagat 20700 tttgaaaatt atgtgaaca gacttgtaat tctagcgcgg tcgtcgataa caattaaag 20760 atacaaacg taatcataga tgaatgttac ggagccccag gatctccaac aaatttggaa 20820 tttatta caggatctag caaggaat tgtgccatta aagcgttgat gcaattgacg 20880 actaaggcca ctactcaaat agcacctaga caagttgctg gtacaggagt tcagttttat 20940 atgattgtta tcggtgttat atattggca gcgttgttta tgtactatgc caagcgtatg 21000 ttgttcacat ccaccaatga taaaatcaaa cttattttag ccataagga aaacgtccat 21060 tggactactt acatggacac attctttaga acttctccga tggttattgc taccacggat 21120 atgcaaaact gaaaatatat tgataatatt ttaatagatt aacatggaag ttatcgctga 21180 tcgtctagac gatatagtga aaaaataat agcggatgaa aaatttgtag attttgttat 21240 acacggtcta gagcatcaat gtcctgctat acttcgacca tttattaggt tgtttattga 21300 tatactatta ttgttatag taatttatat ttttacggta cgtctagtaa gtagaaatta 21360 tcaatgttg tggtggtgc tagtcatcac attackattatt ttatactata 21420 atagtactag actgacttct aaaaaacatc tcacccgcca taaataatg cttgatatta 21480 aagtcttcta tttctacac tattccatct gtggaaaata atactcgac attatcgcta 21540 attgacacat cggtgagtga tatgcctata aagtaataat cttctttggg cacatatacc 21600 agtgtaccag gttctacaa cctatttact gtgctcctg tagcatactt ttctttacc 21660 ttgagaatat ccatcgtttg cttggtcaat agcgatatgt gattttttat caaccactcg 21720 aaaagtaat tggagtgttc atatcctcta cgggctatg tctcatggcc gtgtatgaaa 21780 tttaagtaac acgactgtgg tagattgtt ctatagagcc ggttgccgca atagataga 21840 actaccaata tgtctgtaca atgttaaac atttaatgat taacagaaa aacaatgttc 21900 gttctgggaa tagaaccag attaaaaca aattcgttag atatatgcc acgtttatac 21960 atggaata aaataacac agtttgaaa atacagtat catttaaca tttaacttgc 22020 ggggttaatt tcacaactt actgttttg aactgttcaa atatagcat cgatccatga 22080 gaatacgtt tagccgcctt taatagagga aatcccaccg cctttctgga tctcaccac 22140 gacgatagtt ctgaccagca actcattctct tcatcatcca cctgttttaa catatatag 22200 gcaggagata gatatccgtc attgcaat tccttctcgt agcacaca tctaatattg 22260 aaaatctc cattctctc tctgcattta tatcttgtt tcggtggctg attaggctgt 22320 agtcttggtt taggctttgg tatatcgttg ttgaatctat ttggtcatt aaatctttca 22380 tttctcctg gtatattttct atcacctcgt ttggttgat ttttgtctat attatcgttt 22440 gtaacatcgg tacggtatt catttatcac aaaaaaact tctctaatg agtctactgc 22500 tagaaaacct catcgaagaa gataccatat ttttgcagg aagtatatct gagtatgatg 22560 atttacaaat ggttattgcc ggcgcaaat ccaatttcc aagatcttg ctttctattt 22620 ttatatagt acctagaacg atgtcaaat atgagttgga gttgatcat aacgaaata 22680 tcacaggagc aatgtttacc acatgtata atatagaaa caatttgggt ctaggcgatg 22740 aaactaac tattgaagcc attgaaact atttcttgga tcctacaat gaggttatgc 22800 ctcttatcat taatatacg gatatgactg ccgtcattcc taaaaaagt ggtaggagaa 22860 agaataagaa catggttatt ttccgtcaag gatcatcacc tatctgtgt attttcgaaa 22920 ctcgtaaaa gattaatatt tataaagaa atatggaatc cgcgtcgact gagtatacac 22980 ctatcggaga siaaggct ttgatatcta aatgcggg aattaatgtc ctgaatgtgt 23040 attctccttc attcatcatg agattgaatg attctcgg attcatcat aaaaataac 23100 taggaaact tagtactaat aaggaactag aatcgtatag ttctagccct cttcagaac 23160 ccattaggtt aaatgatttt ctgggactat tggaatgtgt taaaaagaat attcctctaa 23220 cagatattcc ghaaaggat tgattactat aaatggagaa tgttcctaat gtatacttta 23280 atcctgtgtt tatagagccc acgtttaaac attctttatt aagtgtttat aaacacagat 23340 taatagtttt atttgaagta ttcgttgtat tcattctaat atatgtattt tttagatctg 23400 aattaaatat gttcttcatg cctaaacgaa aatacccga tcctattgat agattacgac 23460 gtgctaatct agcgtgtgaa gacgataaat taatgatcta tggattacca tggatgacaa 23520 ctcaaacatc tgcgttatca ataatagta aaccgatagt gtataaagat tgtgcaaagc 23580 ttttgcgatc aataaatgga tcacaaccag tatctcttaa cgatgttctt cgcagatgat 23640 gattcattt ttaagtattt ggctagtcaa gatgatgaat cttcattatc tgatatattg 23700 caaatcactc aatatctaga ctttctgtta ttattattga tccaatcaaa aaataaatta 23760 gaagccgtgg gtcattgtta tgaatctctt tcagaggaat acagacaatt gacaaaattc 23820 acagactctc aagattttaa aaaactgttt aacaaggtcc ctattgttac agatggaagg 23880 gtcaaactta ataaaggata tttgttcgac tttgtgatta gtttgatgcg attcaaaaaa 23940 gaatcagctc tagctaccac cgcaatagat cctgttagat acatagatcc tcgtcgcgat 24000 atcgcatttt ctaacgtgat ggatatatta aagtcgaata aagtgaacaa taattaattc 24060 tttattgtca tcaaaaattg aaattttatt tttttttttt ggatataaa tatactagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat 24180 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgacct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct tcgccccgcta 24360 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg 24540 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg 24720. 24720. 24720. 24720. 24720. 24720. 24720. 24720. 24720 gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga 24840 cgagctgtac aagtccggac tcaggtctag acggattcag atgacacttc acatgagcga 24900 aaaatacatc gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact 24960 gatgccttct gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg 25020 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta 25080 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg 25140 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat 25200 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga 25260 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt 25320 cgtattcgtc ccgccaatct ccggtcgcta atgatagtat tatatttttt atctaaaaaa 25380 ctaaaaaataa acattgatta aattttaata taatacttaa aaatggatgt tgtgtcgtta 25440 gataaaccgt ttgtattt tgaggaatt gatagacga accagaagt 25500 gcaatgagg tcgcaaaaa actgccgtat caggacagt taaaactatt actaggagaa 25560 ttatttttc ttagtaagtt acagcgacac ggtatattag atggtgccac cgtagtgtat 25620 atcatctg ctcccggtac acatacgt tatttgagag atcatttcta taatttagga 25680 gtgatcatca aatggatgct aattgacggc cgccatcatg atcctatttt aaatggattg 25740 cgtgatgtga ctctagtgac tcggttcgtt gatgaggaat atctacgatc catcaaaaa 25800 caactgcatc cttctaagat tattttat tctgatgtga gatccaacg aggaggaat 25860 gaacctagta cggcggattt actaagtaat tacgctctac aaatgtcat gattagtatt 25920 ttaaaccccg tggcatctag tcttaatg agatgcccgt tccagatca atgatcag 25980 gactttatta tcccacgg taaaaatg ttacacctt ttgctccttc atattcagct 26040 gaatgagat tattagtat tttaccggt gagaacatga gactgactcg agttaccaaa 26100 ttagacgctg taaatttaga aaaaagatg tactacctta attagatcgt ccgtaacaa 26160 gtagttgtta actttgatta tcctaatcag gaatatgact attttcacat gtactttatg 26220 ctgaggaccg tgtactgcaa taaaacattt cctactacta aagcaaaggt actatttcta 26280 caacaatcta tatttcgttt cttaaatatt ccaacaacat caactgaaaa agttagtcat 26340 gaaccaatac aacgtaaagt atctagcaaa gattttatgt ctaaaaacag aaatagcaag 26400 agatccgtac gcagtaataa atagaaatag ggataacagg gtaat 26445 <210> 5 <211> 26077 <212> DNA <213> Vaccinia virus <400> 5 tagggataac agggtaatta ttatattttt tatctaaaaa actaaaaata aacattgatt 60 aaattttaat ataatactta aaaatggatg ttgtgtcgtt agataaaccg tttatgtatt 120 ttgaggaaat tgataatgag ttagattacg aaccagaaag tgcaaatgag gtcgcaaaaa 180 aactgccgta tcaaggacag ttaaaactat tactaggaga attatttttt cttagtaagt 240 tacagcgaca cggtatatta gatggtgcca ccgtagtgta tataggatct gctcccggta 300 cacatatacg ttatttgaga gatcattctct ataatttagg agtgatcatc aaatggatgc 360 taattgacgg ccgccatcat gatcctattt taaatggatt gcgtgatgtg acttagtga 420 ctcggttcgt tgatgaggaa tatctacgat ccatcaaaa acactgcat ccttctaaga 480 ttattttaat ttctgatgtg agatcaaac gaggaggaa tgaactagt acggcggatt 540 tactaagtaa ttacgctcta caaatgtca tgattagtat ttaacccc gtggcatcta 600 gtcttaaatg gagatgcccg tttccagatc aatggatca ggacttt atcccacg 660 gtataaaat gttacaacct ttgctcctt catattcagc tgaatgaga ttattaagta 720 tttataccgg tgagaacatg agactgactc gagttaccaa attagacgct gtaaattatg 780 aaaaaagat gtactacctt aagatcg tccgtaacaa agtagttgtt aactttgatt 840 atcctaatca ggaatatgac tatttcaca tgtacttta gctgaggacc gtgtactgca 900 aaaacatt tcctactact aaagcaagg tactatttct acaacaatct atatttcgtt 960 tcttaatat tccaaca tcaactgaaa aagttagtca tgaaccaata caacgtaag 1020 tattctagcaa nationaltttg tctaaaaaca gaatagcaa gagatccgta cgcagtaata 1080 atagaaacg tactactgag atatactacc gatatagagt atatgattt agttacttta 1140 aaccgtta gataaaat tgattctg aaaactgtgt ttcaggtatt taacgaatca 1200 tccataaatt atactccggt tgatgatgat tatggagaac caatcattat aacatcgtat 1260 cttcaaaag gtcataacaa gtttcctgta aattttctat acatagatgt ggtaatatct 1320 gacttatttc ctagctttgt tagactagat actacagaaa ctaatatagt taatagtgta 1380 ctacaacag gtgatgtaa aaagactctt cgtctccca aaatgttaga gacggaata 1440 gttgtcaaga ttcttatcg ccctaatata ccattaaaaa ttgttagat ttccccaat 1500 aacatggtaa ctggagtaga gatagccgat agatctgtta ttcagtcgc tgattaatca 1560 attagtag attagtag acattata taatcaata tattactta tatcttgtttt 1620 agaaaaatgc taatattaaa atagctaacg ctagtaatcc aatcggaagc catttgatat 1680 ctataatagg gtatctaatt tcctgattca gatagcggac agctatattc tcggtagcta 1740 ctcgtttgga atcaaaca ttatttacat ctaatttact atctgtaatg gaaacgtttc 1800 ccaatgaaat ggtacaatcc gatacattgc attttgttat atttttttt aaagaggctg 1860 gtaacaacgc atcgcttcgt ttacatggct cgtaccaaca samaagggt aatcttgtat 1920 ctattcctat ccgtactatg cttttatcag gataaataca tttacatcgt atatcgtctt 1980 tgttagcatc aagaatgca taaatttgtt cgtccgtcat gataaaaatt taaggttaa 2040 atataactat tatttttata gttgtaataa aagggaaat ttgattgtat actttcggtt 2100 ctttaaaaga aactgacttg ataaaaatgg ctgtaatctc taaggttacg tatagtctat 2160 atgatcaaaa agagataat gctacagata ttatcattag tcatgttaaa aatgacgacg 2220 atatcggtac cgttaaagat ggtagactag gtgctatgga tggggcatta tgtaagactt 2280 gtgggaaaac ggaattggaa tgtttcggtc actggggtaa agtaagtatt tataaaactc 2340 atatagttaa gcctgaattt atttcagaaa ttattcgttt actgaatcat atatgtattc 2400 actgcggatt attgcgttca cgagaaccgt attccgacga tattaaccta aaagagttat 2460 cgggacacgc tcttaggaga ttaaaggata aaatattatc caagaaaaag tcatgttgga 2520 acagtgaatg tatgcaaccg tatcaaaaaa ttactttttc aaagaaaaag gtttgtttcg 2580 tcaacaagtt ggatgatatt aacgttccta attctctcat ctatcaaaag ttaatttcta 2640 ttcatgaaaa gttttggcca ttattagaaa ttcatcaata tccagctaac ttattttata 2700 cagactactt tcccatccct ccgttgatta ttagaccggc tattagtttt tggatagata 2760 gtatacccaa agaaaccaat gaattaactt acttattagg tatgatcgtt aagaattgta 2820 acttgaatgc tgatgaacag gttatccaga aggcggtaat agaatacgat gatattaaaa 2880 ttattctaa taacactacc agtattaatt tatcatatat cacatccggc aaaaataata 2940 tgattagaag ttatatcgtc gcccggcgaa aagatcagac cgctagatct gtaattggtc 3000 ccagtacatc tatcaccgtt aatgaggtag gaatgcccgc atatattaga aatacactta 3060 cagaaaagat atttgttaat gcctttacag tggataaagt taaacaacta ttagcatcaa 3120 accaagttaa attttacttt aataaacgat taaaccaatt aacaagaata cgccaaggaa 3180 agtttattaa aaataaaata catttattgc ctggtgattg ggtagaagta gctgttcaag 3240 aatatacaag tattattttt ggaagacagc cgtctctaca tagatacaac gtcatcgctt 3300 catctatcag agctaccgaa ggatacta tcaaaatatc tcccggaatt gccaactctc 3360 aaaatgctga ttcgacggg gatgaggaat ggatgatatt agaacaaaat cctaaagctg 3420 taattgaaca aagtattctt atgtatccga cgacgttact caaacacgat attcatggag 3480 cccctgttta tggatctatt caagatgaaa tcgtagcagc gtattcattg tttaggatac 3540 aagatctttg tttagatgaa gtattgaaca tcttggggaa atatggaaga gagttcgatc 3600 ctaaaggtaa atgtaaattc agcggtaaag atatctatac ttacttgata ggtgaaaaga 3660 ttaattatcc gggactctta aaggatggtg aaattattgc aaacgacgta gatagtaatt 3720 ttgttgtggc tatgaggcat ctgtcattgg ctggactctt atccgatcat aagtcgaacg 3780 tggaaggtat caactttatt atcaagtcat cttatgtttt taagagatat ctatctattt 3840 acggttttgg ggtgacattc aaagatctca gaccaaattc gacgttcact aataaattgg 3900 aggccatcaa cgtagaaaaa atagaactta tcaaagaagc atacgccaaa tatctcaacg 3960 atgtaagaga cgggaaaata gttccattat ctaaagcttt agaggcggac tatgtggaat 4020 ccatgttatc caacttgaca aatcttaata tccgagagat agaagaacat atgagacaaa 4080 cgctgataga tgatccagat aataacctcc tgaaaatggc caaagcgggt tataaagtaa 4140 atcccacaga actaatgtat attctaggta cttatggaca acagaggatt gatggtgaac 4200 cagcagagac tcgagtattg ggtagagttt taccttacta tcttccagac tctaaggatc 4260 cagaaggaag aggttatatt cttaattctt taacaaaagg attaacaggt tctcaatatt 4320 acttttcgat gctggttgca agatctcaat ctactgatat cgtctgtgaa acatcacgta 4380 ccggaacact ggctagaaaa atcattaaaa agatggagga tatggtggtc gacggatacg 4440 gacaagtagt tataggtaat acgctcatca agtacgccgc caattatacc aaaattctag 4500 gctcagtatg taaacctgta gatcttatct atccagatga gtccatgact tggtatttgg 4560 aaattagtgc tctgtggaat aaaataaaac agggattcgt ttactctcag aaacagaaac 4620 ttgcaaaaaa gacattggcg ccgtttaatt tcctagtatt cgtcaaaccc accactgagg 4680 ataatgctat taaggttaag gatctgtacg atatgattca taacgtcatt gatgatgtga 4740 gagagaaata cttctttacg gtatctaata tagattttat ggagtatata ttcttgacgc 4800 atcttaatcc ttctagaatt agaattacaa aagaaacggc tatcactatc tttgaaaagt 4860 tctatgaaaa actcaattat actctaggtg gtggaactcc tattggaatt atttctgcac 4920 aggtattgtc tgagaagttt acacaacaag ccctgtccag ttttcacact actgaaaaaa 4980 gtggtgccgt caaacaaaaa cttggtttca acgagtttaa taacttgact aatttgagta 5040 agaataagac cgaaattatc actctggtat ccgatgatat ctctaaactt caatctgtta 5100 agattaattt cgaatttgta tgtttgggag aattaaatcc aaacatcact cttcgaaaag 5160 aaacagataa atatgtagta gatataatag ttaatagatt atacatcaag agagcagaaa 5220 taaccgaatt agttgtcgaa tatatgattg aacgatttat ctcctttagc gtcattgtaa 5280 aggaatgggg tatggagaca ttcattgagg acgaggataa tattagattt actgtctatc 5340 taaatttcgt tgaaccagag gattgaatc ttagtaagtt tatgatggtt cttccgggggg 5400 cagccaacaa gggaagatt agtaaattca agatcctat ctctgactat acgggatatg 5460 acgacttcaa tcaaaaaa aagctcaata agatgactgt agaactcatg atctaaag 5520 aattgggttc tttcgatttg gaaaacgtca acgtgtatcc tggagtatgg atacatacg 5580 atatcttcgg tatcgaggcc gctcgtgaat acttgtgcga agccatgtta aacacctatg 5640 gagaagggtt cgattatctg tatcagcctt gtgatctctct cgctagttta ctatgtgcta 5700 gttacgaacc agatcagtg aaaattca agttcggcgc agctagtact cttaagagag 5760 ctacgttcgg agacataa gcattgttaa acgcggctct tcataaaag tcagaaccta 5820 ttaacgaata tagtagctgc cacttttta gcaaggtccc taatagga actgatatt 5880 aaatactt tatcgacttg ggtctctca tgagaatga aaggaacta tctgataaga 5940 tatctctca aagatcaag gatatggaag aacagaga cttttaattc ttcataa 6000 catatttttc tatgatctgt cttttaaacg atggattc cacaatgcg cctctcaagt 6060 ccctcataga atgatacacg taaaaaaat atgacatagg caatgactcc tattttttag 6120 acattagata tgccaaaatc atagccccgc ttcttac tcccgcagca caatgaacca 6180 acacggggctc gtttcgttga tcacatttag ataaaaggc ggttacgtcg tcaaatatt 6240 tactaatatc ggtagttgta tcatctacca acggtatatg ataatatta atattagagt 6300 taggtaatgt atatttatcc atcgtcaaat ttaaaacata tttgaactta acttcagatg 6360 atggtgcatc catagcattt ttataatttc ccaaatacac attattggtt acccttgtca 6420 ttatagtggg agatttggct ctgtgcatat ctccagttga acgtagt aagtatttat 6480 acaaactttt cttatccatt tataacgtac aaatggataa actacttta tcggtaaacg 6540 cgtgtaattt agaatacgtt agaaaagg ctatagtagg cgtacaagca gccaaaacat 6600 cacacttat attctttgtt attatattgg cattagtgc gctattactc tggttttcaga 6660 cgtctgataa tccagtcttt atgaattaa cgagatataa gcgaattaa atacggtta 6720 acgattggaa atcattaacg gatagcaaaaaattaga aagtgataga ggtagacttc 6780 tagccgctgg taaggat atttcgaat tcaatgtgt ggatttcggc gcctatttta 6840 tagctatgcg attggataag aaaacatatc tgccgcaagc tattaggcga ggtactggag 6900 acgcgtggat gttaaaaag gcggcaagg tcgatccatc tgctcacaa tttgtcagt 6960 atttgataaa accaagtct ataatgtta ttacttgtgg taatgagatg ttaatgaat 7020 taggttatag cggttatttt atgtcaccgc attggtgttc cgattttagt atatggaat 7080 agtgttagat aaatgcggta acgaatgttc ctgtaggaa ccatacagc ttagatttaa 7140 cgttaaagat gagcataac ataataaca aaattacaat aaccta acattaata 7200 caacaatcc aaaaaatgaa atcagtggag tagtaacgc gtacatact cctggataac 7260 gtttagcagc tgccgttcct attcagacc aaaaattcgg ttcatgttttcgaagcggt 7320 gttctgcaac aagtcgggga tcgtgttcta catatttggc gggcgttatcc agtatctctgcc 7380 tattgatctt catttcgttt tcgattctgg ctatttcaa aaaatccc gatgatagac 7440 ctccagactt tataatttca tccagatgt tcagcgccgt agtactcta atatatagg 7500 ctgataagct aacatcatac cctcctgtat atgtgaatat ggcatgattt ttgtccatta 7560 caagctcggt tttaacttta ttgcctgtaa taatttctct catctgtagg atatctattt 7620 ttttgtcatg cattgccttc aagacgggac gaagaaacgt aatatcctca ataacgttat 7680 cgttttctac aataactaca tattctacct ttttattttc taactcggta aaaaaattag 7740 aatcccatag ggctaaatgt ctagcgatat ttcttttcgt ttcctctgta cacatagtgt 7800 tacaaaaccc tgaaaagaag tgagtatact tgtcatcatt tctaatgttt cctccagtcc 7860 actgtataaa cgcataatcc ttgtaatgat ctggatcatc cttgactacc acaacatttc 7920 ttttttctgg cataacttcg ttgtccttta catcatcgaa cttctgatca ttaatatgct 7980 catgaacatt aggaaatgtt tctgatggag gtctatcaat aactggcaca acaataacag 8040 gagttttcac cgccgccatt tagttattga aattaatcat atacaactct ttaatacgag 8100 ttatatttc gtctatccat tgttcacat ttacatattt cgacaaaaag atataaaatg 8160 cgtattccaa tgcttctg tttaatgaat tactaaaata tacaaacacg tcactgtctg 8220 gcaataaatg atacttaga atattgtaac aatttattt gtattgcaca tgttcgtgat 8280 ctatgagttc ttctcgaat ggcataggat ctccgaatct gaaacgtat aaataggagt 8340 taggaata atatttgaga gtattggtaa tataaact ctttagcggt atattagtt 8400 tttttctc aatttctatt tttagatgtg atggaaaat gactatttt gtagcattag 8460 tatcatgaac tctaatcaa atctttatat cttcgtcaca cgttagctct ttgaagtttt 8520 taagagatgc atcagttggt tctacagatg gagtaggtgc aacaatttt tgttctacac 8580 atgtatgtac tggagccatt gttttaacta taatggtgct tgtatcgaa aactttaatg 8640 cagatagcgg aagctctcg ccgcgacttt ctacatcgta attggttct aacgccgatc 8700 tctgaatgga tactagtttt ctaagttcta atgtgattct ctgaaaatgt aaatccaatt 8760 cctccggcat tatagatgtg tatacatcgg taaataaac tatagtatcc aacgatccct 8820 tctcgcaaat tctagtctta accaaaaat cgtatataac cacggagatg gcgtatttaa 8880 gagtggattc ttctaccgtt tgttcttgg atgtcatata ggaaactata aagtccgcac 8940 tactgttaag aatgattact aacgcaacta tatagttcaa attaagcatt ttggaaacat 9000 aaaataactc tgtagacgat acttgacttt cgaataagtt tgcagacaaa cgaagaaaga 9060 acagacctct cttaatttca gaagaaaact ttttttcgta ttcctgacgt ctagagttta 9120 tatcaataag aaagttaaga attagtcggt taatgttgta tttcattacc caagtttgag 9180 atttcataat attatcaaaa gacatgataa tattaaagat aaagcgctga ctatgaacga 9240 aatagctata tggttcgctc aagaatatag tcttgttaaa cgtggaaacg ataactgtat 9300 ttttaatcac gtcagcggca tctaaattaa atataggtat atttattcca cacactctac 9360 aatatgccac accatcttca taataataa attcgttagc aaaattatta attttagtga 9420 aatagttagc gtcaactttc atagcttcct tcaatctaat ttgatgctca cacggtgcga 9480 attccactct aacatccctt ttccatgcct caggttcatc gatctctata atatctagtt 9540 ttttgcgttt cacaaacaca ggctcgtctc tcgcgatgag atctgtatag taactatgta 9600 aatgataact agatagaaag atgtagctat atagatgacg atcctttaag agaggtataa 9660 taactttacc ccaatcagat agactgttgt tatggtcttc ggaaaaagaa tttttataaa 9720 ttttccagt attttccaaa tatacgtact taacatctaa aaaatcctta atgataatag 9780 gaatggataa tccgtctatt ttataaagaa atacatatcg cacattatac ttttttttgg 9840 aaatgggaat accgatgtgt ctacataaat atgcaaagtc taaatatttt ttagagaatc 9900 ttagttggtc caaattcttt tccaagtacg gtaatagatt tttcatattg aacggtatct 9960 tcttaatctc tggttctagt tccgcattaa atgatgaaac taagtcacta tttttataac 10020 taacgattac atcacctcta acatcatcat ttaccagaat actgatcttc ttttgtcgta 10080 aatacatgtc taatgtgtta aaaaaaagat catacaagtt atacgtcatt tcatctgtgg 10140 tattcttgtc attgaaggat aaactcgtac taatctctttc tttaacagcc tgttcaaatt 10200 tatatcctat atacgaaaaa atagcaacca gtgtttgatc atccgcgtca atattctgtt 10260 ctatcgtagt gtataacaat cgtatatctt cttctgtgat agtcgatacg ttataaaggt 10320 tgataacgaa aatattttta tttcgtgaaa taaagtcatc gtaggatttt ggacttatat 10380 tcgcgtctag tagatatgct tttatttttg gaatgatctc aattagaata gtctctttag 10440 agtccattta aagttacaaa caactaggaa attggtttat gatgtataat ttttttagtt 10500 tttatagatt cttattcta tacttaaaaa atgaaaataa atacaaaggt tcttgagggt 10560 tgtgttaaat tgaaagcgag aaatatcat aaattatttc attatcgcga tatccgttaa 10620 gtttgtatcg taatggcgtg gtcaattaca aataaagcgg atactagtag cttcacaaag 10680 atggctgaaa tcagagctca tctaaaaaat agcgctgaaa ataaagataa aaacgaggat 10740 atttcccgg aagatgtaat aattccatct actaagccca aaaccaaacg agccactact 10800 cctcgtaaac cagcggctac taaaagatca accaaaaagg aggaagtgga agaagaagta 10860 gttatagagg aatatcatca aaactgaa aaaaattctc catctcctgg agtcggcgac 10920 attgtagaaa gcgtggctgc tgtagagctc gatgatagcg acggggatga tgaacctatg 10980 gtacaagttg aagctggtaa agtaaatcat agtgctagaa gcgatctttc tgacctaaag 11040 gtggctaccg acaatatcgt taaagatctt aagaaaatta ttactagaat ctctgcagta 11100 tcgacggttc taggatgt tcagcagct ggtatctcta gatattac ttctatgact 11160 aaagctatta cacacatc tgatctagtc accgagggaa atctaagt tgttcgtaaa 11220 aaagttaaaa cttgtaagaa gtaaatgcgt gcacttttt aataagatgg taaactcttt 11280 accgatata atttttaaa tcctgtatta gatgataatc cagcgtatga ggttttgcaa 11340 catgttaaaa ttcctactca ttcacagat gtagtagtat atgacaac gtgggaagg 11400 gcattaacta gatttatttt tgtgggaagc gattcaaag gacgtagaca atactttac 11460 ggaaaaatgc atgtacagaa tcgcaacgct aaagagatc gtatttttgt tagagtatat 11520 aacgttatga aacgaattta ttgtttta aaaaaaata tAAagaatc gtccacagat 11580 tccaattatc agttggcggtt ttttgtta atgaacta tgtttttat taggattggt 11640 aaatgaat atctttagga gatgaaca gtaggttat taacactaaaaaacac 11700 atagaata gtcccgatga atagttatc aagtttgtag gaaaggacaa agtttcacat 11760 gaatttgttg ttcataagtc tatagacta tataaaccgc tattgaaact gacggatgat 11820 tctagtcccg aagaatttct gttcaacaaa ctaagtgaac gaaaggtata cgaatgtatc 11880 aaacagtttg gtattagaat caaggatctc cgaacgtatg gagtcaatta tacgttttta 11940 tataattttt ggacaaatgt aaagtccata tctcctcttc cgtcaccaaa aaagttaata 12000 gcattaacta tcaaacaaac tgctgaagtt gtaggtcata ctccatcaat ttcaaaaaga 12060 gcttatatgg caacgactat tttagaaatg gtaaaggata aaaattttt agatgtagta 12120 tctaaaacta cgttcgatga attcctatct atagcgtag atcacgttaa atcatctacg 12180 gatggatgat atagatcttt acacaaataa ttacaagacc gataaatgga aatggataag 12240 cgtataaaat ctctcgcaat gaccgctttc tttggggagc taagcacatt agatattatg 12300 gcattgataa tgtctatatt taaacgccat ccaaacaata ccattttttc agtggataag 12360 gatggtcagt ttatgattga ttcgaatac gataattata aggcttctca atatttggat 12420 ctgaccctca ctccgatatt tggagatgaa tgcaagactc acgcatcgag tatagccgaa 12480 aattggcgt gtgcggatat tattaagag gatattagcg atacatca aactactccc 12540 cgtcttaaac gatttataa aaaataccgc atagatcag atactcgtat cagtcgagat 12600 acagaaaagc ttaaaatagc tctagctaaa ggcatagatt acgaatatat aaagacgct 12660 tgttaatag taaatgaaaaaactagtc gtttataata aaacacgata tggatgccaa 12720 cgtagtatca tcttctacta ttgcaacgta tatagacgct ttagcgaaga atgcttcaga 12780 attagacag aggtctaccg catacgaat aaataatgaa ttggaactag tattatta 12840 gccgccatta attackttga caatgtagt gatatctct acgattcagg atcgtttat 12900 tcgatttacc gttactaata aggaggtgt caaattaga actaagattc cattatctaa 12960 ggtacatggt ctagatgtaa aaatgtaca gttagtagat gctatagata acatagtttg 13020 ggaaaagaaa tcattagtga cggaaaatcg tctcacaaa gatgcttgt tgagactatc 13080 gagagaggaa cgtcatatat tttggatta CAagaattat ggatccctcta tccgactga 13140 attagtcaat cttattcaag caaaaacaaa aaactttacg atagacttta agctaaaata 13200 ttttctagga tccggtgccc aatctaaaag ttctttgttg cacgctatta atcatccaaa 13260 gtcaaggcct aatacatctc tggaataga attcacacct agagacaatg aaacagttcc 13320 atatgatgaa ctaataaagg aattgacgac tctctcgcgt catatattta tggcttctcc 13380 agagaatgta attctttc cgcctattaa cgcgcctata aaaaccttta tgttgcctaa 13440 acaagatata gtaggtttgg atctggaaaa tctatatgcc gtaactaaga ctgacggcat 13500 tcctataact atcagagtta catcaaacgg gttgtattgt tattttacac atcttggtta 13560 tattattaga tatcctgtta agagaataat agattccgaa gtagtagtct ttggtgaggc 13620 agttaaggat aagaactgga ctgtatatct cattaagcta atagagcccg tgaatgctat 13680 cagtgataga ctagaagaaa gtaagtatgt tgaatctaaa ctagtggata tttgtgatcg 13740 gatagtattc aagtcaaaga atacgaagg tccgtttact acaactagtg aagtcgtcga 13800 tatgttatct acatatttac caaagcaacc agaaggtgtt attctgttct attcaaaggg 13860 acctaaatct aacattgatt ttaaaattta aaggaaaat actatagacc aactgcaaa 13920 tgtagtattt aggtacatgt ccagtgaacc aattatcttt ggagaatcgt ctatctttgt 13980 agagtataag aaatttaagca acgataagg ctttcctaaa gatatggtt ctggtaagat 14040 tgtgttatat aacggcgtta attatctaaaatatctat tgttttggaat atattaac 14100 acataatgaa gtgggtatta agtccgtggt tgtacctatt aagtttatag cagaattctt 14160 agttaatgga gaatactta aacctagaat tgaataaacc atgaatata ttactcaga 14220 national ggaatcaac atatatcat agtcgacat ttaagagatc aaagcatca 14280 atctttaacg aggaataact atcggatgtg ggacatcaat acgccaataa 14340 tgataaattt agattaatc cagaagttag ttattttacg ataaacgaa ctagaggacc 14400 gttgggaatt ttcaaac acgtcaagac tcttcttatt tcttagtatt gttccaaac 14460 atttttagac gattccaaca aacgaaggt attggcgat gattttgga acggtgctga 14520 cctggaaaaa tactttg gagagattgc gttattgta gcgacggatc cggatgctga 14580 tgctatagct agaggaatg aagatacaa CAATTAAC tctggatta aaaccaagta 14640 ctacaaattt gactacattc aggaactat tcgatccgat acatttgtct ctagtgtcag 14700 agagtattc tattttggaa agtttatat catcgactgg cagtttgcta tccattattc 14760 tttcatccg agacattatg ctaccgtcat gaataactta tccgaacta ctgcttctgg 14820 aggcaaggta ttatcacta ccatggacgg agacaatta tcaaattta cagataaaaa 14880 gactttata attcataaga atttacctag tagcgaaaac tatatgtctg tagaaaaaat 14940 agctgatgat agaatagtgg tatataatcc atcaatg tctactccaa tgactgaata 15000 cattatcaa aagaacgata tagtcagagt gtttaacgaa tacggattg ttcttgtaga 15060 taacgttgat ttcgctacaa ttatagaacg aagtaaaag tttaatg gcgcatctac 15120 atggaagat agaccgtcta caaaaactt ttcgaacta atagaggag ccattaatg 15180 tgaaggttta gatgtcgaag acttacttag ttactatgtt gtttagtct ttctaagcg 15240 gtaaataata atatggtatg ggttctgata tccccgttct aaatgcatta aataattcca 15300 atagagcgat tttgttcct ataggacctt ccaactgtgg atactctgta ttgttaatag 15360 atatattaat acttttgtcg ggtaacagag gttctacgtc ttctaaaaat aaaagtttga 15420 taacatctgg cctgttcata aataaaaact tggcgattct atatactc ttattatcaa 15480 atctagccat tgtcttatag atgtgagcta ctgtaggtgt accatttgat tttctttcta 15540 atactatata tttctcga agaagttctt gcacatcatc tgggaataaa atactactgt 15600 tgagtaaatc agttatttt tttatatcga tattgatgga catttttata gttaaggata 15660 ataagtatcc caaagtcgat aacgacgata acgaagtatt tatactttta ggaaatcaca 15720 atgactttat cagattaaaa ttaacaaaat taaaggagca tgtatttttt tctgaatata 15780 ttgtgactcc agatacatat ggatctttat gcgtcgaatt aaatgggtct agttttcagc 15840 acggcggtag atatatagag gtggaggaat ttatagatgc tggaagacaa gttagatggt 15900 gttctacatc caatcatata tctaaagata tacccgaaga tatgcacact gataaatttg 15960 tcatttatga tattatacg tttgattcgt tcaagaataa acgattggta ttcgtacagg 16020 tgcctccatc attaggagat gatagctatt tgactaatcc gttattgtct ccgtattatc 16080 gtaattcagt agccagacaa atggtcaatg atatgatttt taatcaagat tcatttttaa 16140 aatatttatt agaacatctg attagaagcc actatagagt ttctaaacat ataacaatag 16200 ttagatacaa ggataccgaa gaattaaatc taacgagaat atgtataat agagataagt 16260 ttaaggcgtt tgtattcgct tggtttaacg gcgtttcgga aaatgaaaag gtactagata 16320 cgtataaaaa ggtatctaat ttgatataat gaattcagtg actgtatcac acgcgccata 16380 tactattact tatcacgatg attgggaacc agtaatgagt caattggtag agttttataa 16440 cgaagtagcc agttggctgc tacgagacga gacgtcgcct attcctgata agttctttat 16500 acagttgaaa caaccgctta gaaataaacg agtatgtgtg tgcggtatag atccgtatcc 16560 gaaagatgga actggtgtac cgttcgaatc accaaatttt acaaaaaaat caattaagga 16620 gatagcttca tctatatcta gattaaccgg agtaattgat tataaaggtt ataaccttaa 16680 tataatagac ggggttatac cctggaatta ttacttaagt tgtaattag gagaaacaaa 16740 aagtcacgcg atctactggg ataagatttc caagttactg ctgcagcata taactaaca 16800 cgttagtgtt ctttattgtt tgggtaaaac agatttctcg atatacggg ccaagttaga 16860 atccccggta actaccatag tcggatatca tccagcggct agggaccgcc aattcgagaa 16920 agatagatca tttgaatta tcaacgtttt actgaatta ɣacaagg cacctataaa 16980 ttgggctcaa gggtttattt attaatgctt tagtgaatt ttaacttgtg ttctaaatgg 17040 atgcaactat tagaggtaat gatgttattt ttgtccttaa gactataggt gtcccatcag 17100 catgtagaca aaatgagat ccaagattcg taagcatt taatgcgac gagttaaaaa 17160 gatatattga gatataatcca gatgtacac tattcgaag tcttaggt gaggagcat 17220 actctatagt cagaatttc atggatgtag atttagacgc gtgtctagac gaaatagatt 17280 atttaacggc tattcagat tttatcg aggtgtcaa ctgtgtagct agatcgcgt 17340 ttacagaatg cggtgccatt catgaaatg tataaaatc catgagatct aatttttcat 17400 tgactaagtc tashaataga gataaaaaa gttttcatat tatcttttta vakacgtata 17460 ccactatgga tacattgata gctatgaac gaacactatt agattaagt agatcatctg 17520 aaaatccact aaaagatcg atagacactg ccgtatatag gagaaaaaca actctcggg 17580 ttgtaggtac taggaaaaat ccaattgcg acactattca tgtaatgcaa ccaccccatg 17640 father agattaccta ttcacttacg tggatatga cacatagt tattacttt 17700 ctctacaacg acgattggag gatttagttc ctgataagtt atgggaacca gggtttattt 17760 cattcgaaga cgcttaaaa agagtttca aaatattcat taattctata ataacttta 17820 atgatctcga tgaaaataat tttacaacgg taccactggt catagattac gtaacacctt 17880 gtgcattatg taaaaaacga tcgcataaac atccgcatca actacgttg gaaaatggtg 17940 ctattagaat ttacaaact ggtaatccac atagttgtaa agttaaaatt gttccgttgg 18000 atggtaataa actgtttaat attgcacaa gattttaga cactaactct gttttatta 18060 ccgaacgagg tgaccatata gtttggatta atattcatg gaatttaac agcgagaac 18120 ccttgataac aaaactatt ttgtcataa gatcaact acctaggaa tattcaagcg 18180 aattactctg tccaagaaa cgaagactg tagagctaa catacgagac atgttagtag 18240 attcagtgga aaccgatacc tatccggata aacttccgtt taaaaatggt gtattggacc 18300 tggtagacgg atgttttac tctggagata atgctaaaa atacgtgt actgtatcaa 18360 ccggatttaa atttgacgat aaagttcg tcgagacag tccagaatg gagagttaa 18420 tgaatatcat taacgatc storm cggatgaaaa tagaaaaat agagagttgt 18480 acgaaaaaac tttacttagt tgtttagcg gtgctaccaa aggatgttta acatcttttt 18540 ttggagaac tgcaactgga aagtcgacaa ccaacgttt gttaaagtct gctatcggtg 18600 acctgtttgt tgagacgggt caacaattt taacagatgt attggataaa ggacctaatc 18660 catttatcgc taacatgcat ttgaaagat ctgtattctg tagcgaacta cctgattttg 18720 cctgtagtgg atcaagaa attagatctg acatattaa aaagttgaca gaaccttgtg 18780 tcattggaag accgtgtttc tcataaaa ttaataag aaaccatgcg acaatcatta 18840 tcgatactaa ttacaacct gtttttgata ggatagataa cgcattaatg agagaattg 18900 ccgtcgtgcg attcagaaca cactttctc aaccttctgg tagagaggct gctgaaaata 18960 atgacgcgta cgataaagtc aaactattag acgaggggtt agatggtaaa atacaaata 19020 atagatatag attcgcattt ctatacttgt tggtgaatg gtacagaaaa tatcatgttc 19080 ctattatgaa actatatcct acacggaag agattccgga ctttgcattc tatctcaaaa 19140 taggtactct gttggtatct agctctgtaa agcatattcc attaatgacg gacctctcca 19200 aaaagggata tatattgtac gataatgtgg tcactctcc gttgactact ttcacaga 19260 aaatatccaa gtattttaat tctagactat ttggacacga tatagagagc ttcatcaata 19320 gatataagaa atttgccaat gttagtgatg atatctgca atatatattc atagaggata 19380 tttcatctcc gtaaaatat gctcatatat ttatagaaga tatcacatat ctaaatgaat 19440 accggaatca tagatttatt tgataatcat gttgatagta taccaactat attacctcat 19500 cagttagcta ctctagatta tctagttaga actatcatag atgagaacag aagcgtgtta 19560 ttgttccata ttatgggatc aggtaaaaca ataatcgctt tgttgttcgc cttggtagct 19620 tccagattta aaaaggttta cattctagtg ccgaacatca acatcttaaa aattttcaat 19680 tataatatgg gtgtagctat gaacttgttt aatgacaaat tcatagctga gaatatcttt 19740 attcattcca caacaagttt ttatctctt aattataacg ataacgtcat taattataac 19800 ggattatctc gctacaataa ctctattttt atcgttgatg aggcacataa tatctttggg 19860 aataatactg gagaacttat gaccgtgata aaaaataaaa acaagattcc tttttacta 19920 ttgtctggat ctcccattac taacacacct aatactctgg gtcatattat agatttaatg 19980 tccgaagaga cgatagattt tggtgaaatt attagtcgtg gtaagaaagt aattcagaca 20040 cttcttaacg aacgcggtgt gatgtactt aaggatttgc ttaaggag atatcatat 20100 tacgaaatgc ctgataaga tctaccaacg atagatc acggacgtaa gtttctagat 20160 actagagtag tatattgtca catgtctaaa cttcagaga gagattatat gattactaga 20220 cgacagctat gttatcatga aatgttttgat aaaatatgt ataacgtgtc aatggcagta 20280 ttgggacaac ttaatctgat gatataattta gatacttta ttcaggaca ggataaggaa 20340 ttgtacccaa atctgaaat aaataatggc gtgttatacg gagagaatt ggtaacgtta 20400 aacattagtt ccaatttaa atactttatc aatcggatac agacactca cggaaaacat 20460 tttatatact tttctattc tacatatggc ggattggtaa ttaatatat catgctcagt 20520 aatggatatt ctgaatataa tggttctcag ggaactaatc cacatatgat aaacggcaaa 20580 ccaaaaacat ttgctatcgt tactagtaaa atgaatcgt ctttagagga tctattagat 20640 gtgtataatt ctcctgaaaa cgatgatggc agtcattga tgttttgtt ttcgtcaaac 20700 attatgtccg aatcctatac tctgaaagg gtaaggcata ttggtttat gactaccca 20760 gatacttttt ctcaatacaa ccaatctt ggacgatcta ttagaaaatt ctcttacgcc 20820 gatatttctg aaccagttaa tgtatatctt ttagccgccg tatattccga ttcaatgac 20880 gaagtgacgt cattaacga ttacacacag gatgaattta ttaatgtttt accatttgac 20940 atcaaaagc tgttatatct aaaatttaag acgaaagaa cgaatagaat attackctatt 21000 cttcaagaga tgtctgaaac gtattctct ccaccacatc catcattgt aaagtttta 21060 ttgggagaat tggtcagaca atttttttat ataatttctc gtattaagta taacgactcc 21120 aagttactta aatggttac atcagttata aaaaataag agacgctag gattacata 21180 gatgatattg taaacggtca cttctttgta tcgataaag tatttgataa atctcttta 21240 tacaaatacg aaaacgatat tattacagta ccgtttagac ttcctacga accattgtt 21300 tggggagtta actttcgtaa agaataac gtggtatctt ctccataaaa ctgatgagat 21360 atataagaa aataatgtcg agctttgtta ccaatggata ccttccagtt acatggaac 21420 ctcatgagct gacgttagac aaaaacta atattaggaa tgccgtatat aagacgtatc 21480 tccatagaga attagtggt aaatggcca agaaataga aattcgtgaa gacgtggaat 21540 tacctctcgg cgaatagtt aaatttctg tagttataa cgttccgtgt gtaataacct 21600 acgcgtatta tcacgttggg gatatagtca gaggacatt aacatcgaa gatgaatca 21660 atgtaactat tcaatgtgga gatttaatct gtaaactaag tagagattcg ggtactgtat 21720 catttagcga ttcaagtac tgctttttc gaatggtaa tgcgtatgac atggcagcg 21780 aagtcactgc cgttctaatg gaggctcaac aaggtacga atctagtttt gttttctcg 21840 cgaatatcgt cgactcataa aaagagaat agcggtaagt ataacacga atactatggc 21900 aaattgcg aatgttttat tccctcgat atatttttga taatatgaa aacatgtctc 21960 tctcaatcg vakaaccac tcataaaata gttctcgcgc gctggagagg tagttgctgc 22020 tcgtataatc tccccagaat atatacttc tgtgtcatcg ttcaatttat acggattct 22080 atagttctct gttatatat gcggtttgcc ctcatgatta vakacgaca atagtgttct 22140 gatttagat agttgatcag atgaatgtt tattggcgtt ggaaaatta tccatacagc 22200 gtctgcagag tggttgatag ttgttcctag atatgtaaaa taatccaact tactaggcag 22260 caattgtct agtaaaata ctgaatcaa cggtgcagac gtattggcgg atctaatgga 22320 atccaattga ttaactatct tttgaaata tacattttta tgatccaata cttgtaagaa 22380 tatagaaata atgataagtc catcatcgtg ttttttgcc tcttcatag aactatattt 22440 tttcttattc caatgaaca gattaatctc tccagagtat ttgtacacat ctatcaagtg 22500 attggatcca taatcgtctt cctttcccca atatatatgt agtgatgata acacatattc 22560 attggggaga aaccctccac ttatatcc tcctttaaaa ttaatcctta ctagttttcc 22620 agtgttctgg atagtggttg gtttcgactc attataatgt atgtctaacg gcttcaatcg 22680 cgcgttagaa attgctttt tagtttctat attatagga gatagttgtt gcggcatagt 22740 aaaaatgaaa tgatactgt ttaaaaatag ctcttagt gggaattaca atggatgagg 22800 aagtgatatt tgaaactcct agaaattaa tatctattaa acgaataaaa gatattccaa 22860 gatcaaaaga cacgcatgtg tttgctgcgt gtataacaag tgacggatat ccgttaatag 22920 gagctagaag aacttcattc gcgttccagg cgatattatc tcaacaaaat tcagattcta 22980 tctttagagt atccactaaa ctattacggt ttatgtacta caatgaacta agaaatct 23040 ttagacggtt gagaaaaggt tctatcaaca atatcgatcc tcactttgaa gagttaatat 23100 tattgggtgg taactagat aaaaaggaat ctattaaaga ttgtttaaga agaaattaa 23160 23220 caacacggga taaattattt aataagtat atataagtta ttgcatggcg tgttttta 23280 atcaatcgtt ggaggattta tcgcatacta gtatttacaa tgtagaaatt agaaagatta 23340 aatcattaaa tgattgtatt aacgacgata aatacgaata tctgtcttat atttataata 23400 tgctagttaa tagtaaatga acttttacag atctagtata attagtcaga ttattaagta 23460 taatagacga ctagctaagt ctattatttg caaggatgac tctcaaatta ttacactcac 23520 ggcattcgtt aaccaatgcc tatggtgtca taaacgagta tccgtgtccg ctattttatt 23580 aactactgat aacaaaatat tagtatgtaa cagacgagat agttttctct attctgaaat 23640 aattagaact agaaacatgt ttagaaagaa acgattattt ctgaattatt ccaattattt 23700 gtccaaacag gaaagaagta tactatcgtc attttttct ctatatccag ctactgctga 23760 taatgataga atagacgcta tttatccggg tggcataccc aaaaggggtg agaatgttcc 23820 agagtgttta tccagggaaa ttaaagaaga agttaatata gacaattctt ttgtattcat 23880 agacactcgg ttttttattc atggcatcat agaagatacc attattaata aattttttga 23940 ggtaatcttc tttgtcggaa gaatatctct aacgagtgat caaatcattg atacatttaa 24000 aagtaatcat gaaatcaagg atctaatatt tttagatccg aattcaggta atggactaca 24060 atacgaaatt gcaaaatatg ctctagatac tgcaaaactt aaatgttacg gccaatagagg 24120 atgttattac gaatcattaa aaaaattaac tgaggatgat tgattagaaa atataaatta 24180 atttaccatc gtgtattttt ataacgggat tgtccggcat atcatgtaga tagttaccgt ctacatcgta tactcgacca tctacgcctt taaatcctct atttattgac attaatctat tagtgga attack ttagtaccct caattagttt attack ttttttttag acgatagatc gatggctctt gaaaccaagg ttttccaacc ggactcattg tcgatcggtg agagtcttt ttcattagca tgaatccatt ctaatgatgt atgtttaaac actctaaaca attggacaaa ttcttttgat ttgctttgaa tgatttcaaa taggtcttcg tctacagtag gcataccatt agataatcta gccattataa agtgcacgtt tacatatcta cgttctggag gtgactattg gtgactattg ctcttcctac tatctgacga agacgcct cgttccatgt catatctaaa atgaagatat cattattga gaaaaaacta ataccctcgc 24780. ctccactaga agagaatacg catgttttaa tgcattctcc gttagtgttt gattcttggt 24840. 24840. 24840. 24840. 24840. 24840. 24840. 24840. 24840. 24840 agataccaaa gactttgaaa tatagtaata agatttctat tcctgactga ttaacaaatg gttcaaagac tagacattta ccatgggatg ctaatattcc siacataca tctaaattt 24960 tgacgctttt ctctttaat tcagtaata gagagatatc agccgcacta gcatcccctt 25020 tcaatagttc tcccttttta aaggtatcta atgcggattt agaaaactct ctatctctta 25080 atgaattttt aaatcatta tatagtgttg ctatctcttg cgcgtattcg cccggatcac 25140 gattttgtct ttcaggaag ctacgaacg taaacgtagt agccatacgt ctcagaatttc 25200 taaatgatga tatacctgtt tttatttcag cgagtttagc cttttgataa atttctctt 25260 gctttttcga catattaacg tatcgcatta atactgtttt cttagcgaat gatgcagacc 25320 cttctacgtc atcaaaata gaaaactcgt tattaactat atacgaacat agtcctccta 25380 gtttggagac taattttttt tcatcgacta gacgtttatt ctcaatagt gattggtgtt 25440 gtaaggatcc tggtcgtagt aagttaacca acatggtga ttcttgcaca ctattgacga 25500 taggtgtagc cgataaaaca atcatcttat ggtttttta tgcgatggtc ttggataaaa 25560 aattatatac tgaacgagta ggacggatct taccatcttc tttgattaat gatttagaaa 25620 tgaagttatg acattcatca ataatgacgc atattctact cttggaatta atagttttga 25680 tattagtaaa aaatttattt ctaaaatttt gatcatcgta attaataaaa atacaatcct 25740 tcgttatctc tggagcgtat ctgagtatag tgttcatcca aggatcttct atcaaagcct 25800 ttttcaccaa taagataata gcccaattcg tataaatatc cttaagatgt ttgagaatat 25860 atacagtagt cattgtttta ccgacacccg tttcatggaa caataaaaga gaatgcatac 25920 tgtctaatcc taagaaaact cttgctacaa aatgttgata atccttgagg cgtactacgt 25980 ctgttcccat catttcaaca ggcatattag tagttctgcg caatgcataa tcgatatagg 26040 ccgcgtgtga tttactcatt agggataaca gggtaat 26077 <210> 6 <211> 24671 <212> DNA <213> Vaccinia virus <400> 6 tagggataac agggtaattt agaaaatata aattaattta ccatcgtgta tttttataac 60 gggattgtcc ggcatatcat gtagatagtt accgtctaca tcgtatactc gaccatctac 120 gcctttaaat cctctattta ttgacattaa tctattagaa ttggaatacc aaatattagt 180 accctcaatt agtttattgg tatatttt tttagacgat agatcgatgg ctcttgaaac 240 caaggttttc caaccggact cattgtcgat cggtgagaag tctttttcat tagcatgaat 300 ccattctaat gatgtatgtt taaacactct aaacaattgg acaaattctt ttgatttgct 360 ttgaatgatt tcaaataggt cttcgtctac agtaggcata ccattagata atctagccat 420 tataaagtgc acgtttacat atctacgttc tggaggagta agaacgtgac tattgagacg 480 aatggctctt cctactatct gacgaagaga cgcctcgttc catgtcatat ctaaaatgaa 540 gatatcatta attgagaaaa aactaatacc ctcgcctcca ctagaagaga atacgcatgt 600 tttaatgcat tctccgttag tgtttgattc ttggttaaac tcagccaccg ccttgattct 660 agtatctttt gttctagatg agaactctat attagagata ccaaagactt tgaaatatag 720 tataagatt tctattcctg actgattaac aaatggttca aagactagac atttaccatg 780 ggatgctaat attcccaaac atacatctat aaatttgacg cttttctctt ttaattcagt 840 aatagagag atatcagccg cactagcatc ccctttcaat agttctccct ttttaaaggt 900 atctaatgcg gatttagaaa actctctatc tcttaatgaa tttttaaaat cattatatag 960 tgttgctatc tcttgcgcgt atcgcccgg atcacgattt tgtctttcag gaaagctatc 1020 gaacgtaaac gtagtagcca tacgtctcag aattctaaat gatgatatac ctgttttat 1080 ttcagcgagt ttagcctttt gataaatttc ttcttgcttt ttcgacatat taacgtatcg 1140 cattaatact gttttcttag cgaatgatgc agacccttct acgtcatcaa aatagaaaa 1200 ctcgttatta actatatacg aacatagtcc tcctagtttg gagactaatt cttttcatc 1260 gactagacgt ttattctcaa atagtgattg gtgttgtaag gatcctggtc gtagtaagtt 1320 aaccaacatg gtgaattctt gcacactatt gacgataggt gtagccgata aacaaatcat 1380 cttatggtttt tttaatgcga tggtcttgga taaaaatta tatactgaac gagtaggacg 1440 gatcttacca tcttctttga ttaatgattt agaaatgaag ttatgacatt catcaataat 1500 gacgcatatt ctactcttgg aattaatagt tttgatatta gtaaaaaatt tatttctaaa 1560 attttgatca tcgtaattaa taaaaataca atccttgtt atctctggag cgtatctgag 1620 tatagtgttc atccaaggat cttctatcaa agcctttttc acaataaga taatagccca 1680 attcgtataa atatccttaa gatgtttgag aatatataca gtagtcattg ttttaccgac 1740 acccgtttca tggaaata aaagagaatg catactgtct aatcctaaga aaactcttgc 1800 tacaaaatgt tgataatcct tgaggcgtac tacgtctgtt cccatcattt caacaggcat 1860 attagtagtt ctgcgcaatg cataatcgat ataggccgcg tgtgatttac tcatttatga 1920 1980 2040 atagagagc ttcgtatact ctattcgaaa actctaagaa cttattaatg aattccgtat 2100 ccactggatt gtttaaaata ctaaattgaa cactgttcac atccttccaa gaagaagact 2160 2220 ccaccataat ggcgctatct ttcaaccagc tatcgcttac gcatttagc agtctaacat 2280 ttttaaagg actacaat attctcatag tattcgattac acctctaccg ataaagttg 2340 gaagttttaat atacaat tttcgtttta aaatcaa taatggtcga aacacgtcga 2400 aggttaacat cttataatcg ctaatgtata gattgttttc agtgagatga ttattagat 2460 taatagcatc tcgttcacgt ttgacagtt tattgcgtgc gctgaggtcg gcaacacgg 2520 cgtccgcttt agtactccctc ccataatact ttacgctatt aatctttaaa atttcataga 2580 ctttatctag atcgctttct ggtaacatga tatcatgtgt aaaagtttt aacatgtcgg 2640 tcggcattct atttagatca ttactctag aaatctgaag aaagtaatta gctccgtatt 2700 ccagactagg taatgggctt ttacctagag acgattaag ttctggcaat gtttcataaa 2760 atggagaag gatagcgtt cccctcccgga tattttttac aatttcatcc atttacact 2820 ctatagtttg tttcattat tattat tatctcccat aatcttgta atacttacc 2880 cttgatcgta agatacctta tacaggtcat tacatacac taccattgt tttgtacat 2940 atagattgg atggttgaca tccatggtgg aaaactac tcgaacagat agtttatctt 3000 tccccctaga tacattagcc gtaatagttg tcggcctaaa gaatatcttt ggtgtaaagt 3060 taaaagttag ggttcttgtt ccattattgc ttttgtcag tagttcatta taattctcg 3120 agatgggtcc gttctctgaa tatagaacat catttccaaa tctaacttct agtctagaaa 3180 tatatcggt cttattctta aaatctattc ccttgatgaa gggatcgtta atgaacaaat 3240 ccttggcctt tgattcggct gatctattat ctccgtta gacgttacgt tgactagtcc 3300 aaagacttac aggaatagat gtatcgatga tgttgatact atgtgatatg tgagcaaaga 3360 ttgttctctt agtggcatca ctatatgttc cagtaatggc ggaaaacttt ttagaaatgt 3420 tatatataaa agaatttttt cgtgttccaa acattagcag attagtatga agataaacac 3480 tcatattatc aggaacatta tcaattttta catacacatc agcatcttga atagaaacga 3540 taccatcttc tggaacctca acaatctcgg cagactccgg ataaccagtc ggtgggccat 3600 cactaacaat aactagatca tccaacaatc tactcacata tgcatctata taatcttttt 3660 catcttgtga gtaccctgga tacgaaataa atttattatc cgtatttcca tataaggtt 3720 tagtataaac agagagcgat gttgccgcat gaacttcagt tacagtcgcc gttggttggt 3780 ttatttgacc tattactctc ctaggtttct ctataaacga tggtttaatt tgtacattct 3840 taaccatata tccaataaag ctcaattcag gaacataaac aaattctttg ttgaacgttt 3900 caaagtcgaa cgaagagtca cgaataacga tatcggatac tggattgaag gttaccgtta 3960 cggtaatttt tgaatcggat agtttaagac tgctgaatgt atcttccaca tcaaacggag 4020 ttttaatata aacgtatact gtagatggtt ctttaatagt gtcattagga gttaggccaa 4080 tagaaatatc attaagttca ctagaatatc cagagtgttt caaagcaatt gtattattga 4140 tacaattatt atataattct tcgccctcaa tttcccaaat aacaccgtta cacgaagaca 4200 ccgatacgta attaatacat ttatatccaa catatggtac gtaaccgaat cttcccatac 4260 ctttaacttc tggaagttcc aaactcagaa ccaaatgatt aagcgcagta atatactgat 4320 ccctaatttc gaagctagcg atagcctgat tgtctggacc atcgtttgtc ataactccgg 4380 atagagaaat atattgcggc atatataaag ttggaatttg actatcgact gcgaagacat 4440 tagaccgttt aatagagtca tccccaccga tcaaagaatt aatgatagta ttatcattt 4500 tctatttaaa atggaaaaag cttacaataa actccgtaga gaaatatcta taatttgtga 4560 gttttcctta aagtaacagc ttccgtaaac gccgtcttta tctcttagta agtttattgt 4620 atttataacc ttttccttat cttcatagaa tactaaaggc aacaaagaaa tttttggttc 4680 ttctctaaga gctacgtgag acttaaccat agacgccaac gaatccctac atattttaga 4740 acagaaatac ccaacttcac cacccttgaa tgtctcaata ctaataggtt taaaaaccaa 4800 atcttgatta caaaaccaac acttatcaat tacactattt gtcttaatag acacatctgc 4860 catagattta taatactttg gtagtataca agcgagtgct tcttctttag cgggcttaaa 4920 gactgcttta ggtgctgaaa taaccacatc tggaaggctt actcgcttag ccatttaatt 4980 acggaactat ttttttatac ttctaatgag caagtagaaa acctctcatc tacaaaaaaca 5040 tactcgtgtc cataatcctc taccatagtt acacgttttt tagatctcat atgtgctaaa 5100 aagttttcc atactaattg gttactatta tttttcgtat aatttttaac agtttgaggt 5160 tttagatttt tagttacaga agtgatatcg aatattttat ccaaaaaagaa tgaataatta 5220 attgtcttag aaggagtgtt ttcttggcaa aagaatacca agtgcttaaa tatttctact 5280 acttcattaa tcttttctgt actcagattc agtttctcat cttttacttg attgattatt 5340 tcaaagacta acttataatc ctttttattt attctctcgt tagccttaag aaaactagat 5400 acaaaatttg catctacatc atccgtggat atttgatttt tttccatgat atccaagagt 5460 tccgagataa tttctccaga acattgatga gacaataatc tccgcaatac atttctcaaa 5520 tgaataagtt tattagacac gtggaagttt gactttttt gtacctttgt acatttttga 5580 aataccgact cgcaaaaaat acaatattca tatccttgtt cagatactat accgttgtgt 5640 ctacaaccgc tacataatcg tagattcatg ttaacactct acgtatctcg tcgtccaata 5700 ttttatataa aaacatttta tttctagacg ttgccagaaa atcctgtaat atttttagtt 5760 ttttggggctg tgaataaagt atcgccctaa tatggttacc gtcctccgcc aatatagtag 5820 ttaaattatc cgcacatgca gaagaacacc gcttaggcgg attcagtaca atgttatatt 5880 tttcgtacca actcatttaa atatcataat ctaaaatagt tctgtatatat gtctagcgct 5940 atatattga tcataatcct gtgcataaat window caatgtctcg aaatcatcga 6000 catggcttct tccatagtta gagatcgtc gtcaagtta gcaacgtgat tcatcacat 6060 ttgctgtttt gaggcagca attackgaacc gtcgccattc aaccattcat aaaaaccatc 6120 gtctgaatcc attgataatt tcttgtactg gttttgaga gctcgcatca atctagcatt 6180 tctagctccc ggattgaaa cagaaagagg atcgtacatc cagggtccat tttctgtaaa 6240 taatcgta taatgtccct tcaagagat atcagacgat ccacaatca agaattgatc 6300 tccgagtttg taacaactg cggactttaa cctatacatg ataccgttta gcatgatttc 6360 tggtgatacg tcaatcggag tatcatctat tagagatcta aagccggtgt aacattctcc 6420 accaaacata ttcttattct gacgtcgttc tacataaac atcattgctc cattaacgat 6480 aacaggggaa tgacagcac tacccac attagttccc aatggatcaa tgtgtgtaac 6540 tccagaacat cttccatagc ctagttagg aggagcgaac accactctc cactattgcc 6600 atcgaatgcc atagataaa tatccttgga attgatagaa atcggactgt cggatgttgt 6660 gatcatcttc ataggatta caactatgta tggtgccgcc tgaagtttca tatcgtact 6720 gatgccgttt ataggtctag cacagaac caacgtaggt ctaaatccaa ctatagacaa 6780 atagaagcc atatctgtt cctcatctgt cataacttga gagcatccag tatgaataat 6840 cttcattaga tgggatcta ccgcatcatc atcgttacaa taaaaaattc ccattctaat 6900 gttcataatt gcttttctaa tcatggtag catgtttgct ctctgaatct ctgtggaaat 6960 tagactgat acacctgtaa tcactatcgg attatcctcc gtaagacgat taaccaaca 7020 catataatta tagacttta ctttctaaa ttcataagt tgctggatta ggctataggt 7080 gtctccatgt acatacgcgt tctcgagcgc aggaagttta ataccgaata gtgccatcag 7140 ataggatga atatagtaat tagttctctgg ttttcttaa aaaagaca aatcttgtga 7200 actagacata tcggtaaaat gcatggattg gatcgtgta gtcgacagaa gatatgatg 7260 attagatgga gagtatattt tatctactc ttgagttgg tcaccgattc taggacttagc 7320 tcgagaatga ataagtacta aaggatgagt acatttcaca gaaacactag cattgttcaa tgtgctcttt acatgggtaa ggagttgaaa tagctcgttt ctattgttc tgacaatatt tagtttattc father gcatatcctg father ttagatgtgt catacttgtt owner owner ttgcattccc atcatttctc aatctcgtac tccaatcatg 7560. cgtggatgct acttcgtcga tggaaaccat acaatccttt ttgataggct gttgagattg attatttcct gcacgtttag gtttggtacg ttgatttcta gcccctgcgg atataaagtc 7680 7740. atcgtctaca attttggata atgaattgca tacactaca gacaaagatt tatcagaagt 7800. 7800. 7800. 7800. 7800. 7800. 7800. 7800. tgcgttagat gttaaaaaaa catcgctatt gaccacggct tccattattt atattcgtag tttttactcg aaagcgtgat tttaatatcc aatcttatta cttttggat cgttcaaaac ctttgactaa ttgtagaatt tgatctattg ccctacgcgt atactccctt gcatcatata 7980. cgttcgtcac cagatcgttt gtttcggcct gaagttggtg catatctctt tcaacattcg acatgagatc cttaagggcc atatcgtcta gattttgttg agatgttgct cctggatttt 8100 gttgtgctgt tgtacatact gtaccaccag taggtgtagg agtacataca gtggccacaa 8160 taggaggttg aggaggtgta accgttggag tagtacaaga atattcca tccgattgtt 8220 gtgtacatgt agttgttggt aacgtctgag aaggttgggt agatggcggt gtcgtcgtct 8280 tttgatcttt attaattta gagatatat cctgaacagc attgctcggc gtcaacgctg 8340 gaaggagtga actcgccggc gcatcagtat ctgcagacag ccaatcaaa agattagaca 8400 tatcagatga tgtattagtt tgttgtcgtg gttttgtgt aggagcagta ctactaggta 8460 gagaatagg agccgatgta gtgtcggaa ccggaaccgg ctgtggagtt attgaatag 8520 ttggttgtag cggttggata ggctgtctgc tggcggccat catattatct ctagctagtt 8580 gttctcgcaa ctgtctttga taatacgact cttgagactt tagtcctatt tcaatcgctt 8640 catccttttt cgtatccgga tccttttctt cagaataata gattgacgac tttggtgtag 8700 aggattctgc cagcccctgt gagaacttgt taagaagtc catttaggc tttaaaattg 8760 aattgcgatt atagattaa atggcagaca cagacgact tatcgactat gatccgatg 8820 atctcactga atcgaggat gatgagaag aggaag tggagtca ctagaaacta 8880 gtgatataga tcccaatct tcttataaga tgtagaatc agcatccact catatagaag 8940 atgcgcattc caatcttaa catatagg atcatatatc tgctttaaa cgacgctata 9000 ctagacgtat aagtctattt gaatagcgg gtataatagc agaagctat aacttgcttc 9060 aacgaggaag attackcta gttcagaat ttctgacga acgatgaag caaatatgc 9120 tacatgtaat atacaagag atagaggagg gttcttgtcc tatagtcatc gaaagaacg 9180 gagaattgtt gtcggtaaac gattttgaca aagatggtct aaattccat ctagactata 9240 ttcaaat ttggaactt caaaacgat attagaattt atacgaatat cgttctctaa 9300 atgtcacaat caagtctcgc atgttcagca atttattgtc gtactttata tcgtgttcat 9360 taacgatatc ttgcaaata gtaatgattc tatctctcctt cgatagatat tcttcagaga 9420 ttattgtctt atattctttc ttgttatcag atatgaattt gatagactt tgaacattt 9480 tgatacccgt ctgtttaatt ttttctacag atattttagt tttggcagat tctatcgtat 9540 ctgtcaatag acatccaaca tcgacattcg acgtcaattg tctataaatc aacgtaaaa 9600 ttttagaaat aacattagcg aattgttgtg cattgatgtc gttatctga aacagtatga 9660 ttttaggtag cattttctta aaaagaagaa cgtatttatt gttactcagt tgaacagatg 9720 atatatccag attactaacg catctgattc cgtataccaa actttcagaa gaaatggtgt 9780 acaattgttt gtattcattc aatgtctcct tttcagaaat tagtttagag tcgaatactg 9840 caataatttt caagagatag ttttcatcag ataagatttt atttagtgta gatatgataa 9900 aactattgtt ttgttggaga acttgatacg ccgcgttctc tgtagtcgac gctctcaaat 9960 gggaaacgat ctccattatt tttttggaat cggatacaat atcttcggta tcttgacgca 10020 atctagtata catagagtta agaaatta gagtttgtac attgagcaac atgtctctaa 10080 atgtggctac aaacttttcc tttttcacat catctagttt attatatacc gatttcacaa 10140 cggcaccaga tttaaggaac cagaatgaaa aactctgata actacaatat ttcatcatag 10200 ttacgatttt atcatcttct atagttggtg tatagcgca tacctttttc tccaagactg 10260 gaaccaacgt cataaaaatg tttaaatcaa aatccatatc aacatctgat gcgctaagac 10320 cagtctcgcg ttcaagatta tctttactaa tggtgacgaa ctcatcgtat agaactctaa 10380 gttgtccat tatttattta cagatttagt tgtttaattt atttgtgctc ttccagagtt 10440 gggatagtat tttctaacg tcggtattat attattagga tctacgttca tatgtatcat 10500 aatattaatc atccacgttt tgataaatct atctttagct tctgaaataa cgtatttaaa 10560 caaaggagaa aaatttag ctacggcatc agacgcaata acattttttg taaatgtaac 10620 gtatttagac gacagatctt cgttaaaaag ttttccatct atgtagaatc catcggttgt 10680 taacaccatt cccgcgtcag attgaatagg agtttgaata gtttgttttg gaaatagatc 10740 cttcaataac ttatagttgg gtgggaaaaa atcgatttta tcactagact cttcttttt 10800 tactatcatt acctcatgaa ctatttcttg aatgagtata tgtattttct ttcctatatc 10860 ggacgcgtc attggaaaat ataccatgtc gttaactata agaatatttt tatcctcgtt 10920 tashaactga attaatcag atgtagttcg taaacgaact atacatcac 10980 tctaactata tgatatccac tagtttcctt tagtcgttta tatcttgtt ccatattagc 11040 agtcattcca tcatttaaga aggcgtcaaa gataaggg agaatgaca ttttggattc 11100 tgttacaact ttaccaaat taggatata cggacttact atcttttt caacgtcaat 11160 ttgatgaaca cacgatgaaa atgtacttcg atgagattga tcatgtagaa aaaaaaagg 11220 gatacaata ttccgcatat catgaataat attaagaat cccaccttat tatattccc 11280 caaaggatcc atgcacgtaa acattatgcc gttatcatta ataagactt ctttctcatc 11340 ggatctgtaa aagttgttac tgatttttt cattccagga tctagataat taataatgat 11400 gggttttcta ttcttattct ttgtattttg gcatatccta gaccagtaaa cagttttccac 11460 ttggtaaaa tcagcagact tttgaacgct attaaacatg gcattaatgg caataactaa 11520 aaatgtaaaa tattttcta tgttaggaat atggttttc actttaatag atatatggtt 11580 tttggccaaa atgatagata tttttttatc cgaggatagt aaaatattat tagtcgccgt ctctaaaa atgaagctag tctcgatatc caattttatt ctagaattga taggtcgc caaatgtacc ttatacgtta tatctccctt gatgcgttcc atttgtgtat ctatatcgga cacaagatct gtaaatagtt ttacgttatt aatcatcacg gtatcgccgt cgctagataa cgctaatgta ccatccaagt cccaaatgga gagatttaac tgttcatcgt tgaataaa atgattaccg gtcatattaa taagtgttc atcgtatcta gataacaacg acttataatt aatgtccaag tcttgaactc gctgaatgat cttttttaac ccagttagtt ttagattggt acgaaatata ttgttaaact ttgattctac agtaatgtcc aaatctagtt gtggaaatac ttccatcaac attgtttcaa acttgataat attattatct acatcttcgt acgatccaaa ttccggata gatgtatcgc acgctctagc cacccagata accaaaaagt cacacgctcc aggatataca ttgtataaaa agctatcgtt ttttagtagt gtttttttct aggtatac average aaaatagtat tatcaacgta actatattcc aaattattct tatgagaata throwing tcgtcctta tatctaca atttcctaa tatcccttta attgagtcat tcgaagcgtc father gtctcttaac tattttccggc tgttgtatat ttaaatgact tcgtaaaaaa father gcgacttctc atctatgtaa tcatatggag tgagatatag ggctcgttct acctcctgcc ccttacccac ctgtaatacc aattgcggac ttactatata tcgcatattt atatcgtggg gtaaagtgaa aatctactac cgatgatgta agtcttacaa tgttcgaacc agtaccagat cttaatttgg aggcctccgt agaactaggg gaggtaaata 12720. 12720. 12720. 12720. 12720. 12720. 12720. 12720 tattcgccca tagatctaag gaatgatgaga gaaaactagc actacgattc tttttacaaa gactttattt tttagatcat agagagattc attatttgtt cagatgcgtt gacgctgtaa aagacgtcac tattaccaaa aaaataca ttatcgtggc gccttatata gcacttttaa ctatcgcatc aaaaggatgc aaacttacag aaacaatgat tgaagcattc tttccagaac tatataatga acatagtaag aaatttaaat tcaactctca agtatccatc atccaagaaa 13020 aactcggata ccagtttgga aactatcacg tttatgattt tgaaccgtat tactctacag 13080 tagctctggc tattcgagat gaacattcat ctggcatttt tatatccgt caagagagtt 13140 atctggtaag ttcattatct gaaataacat atagatttta tctaattaat ctaaaatctg 13200 atcttgttca atggagtgct agtacgggcg ctgtaattaa tcaaatggta aatactgtat 13260 tgattacagt gtatgaaaag ttacaactgg tcatagaaaa tgattcacaa tttacatgtt 13320 cattggctgt ggaatcagaa cttccaataa aattacttaa agatagaaat gaattattta 13380 caaaattcat caacgagtta aaaaagacca gttcattcaa gataagcaaa cgcgataagg 13440 atacgctact aaaatatttt acttaggact ggagttagaa tttatagacg actcatttcg 13500 tttatcattg ttactattat tactattact atcattatta gtgttggcat tattagtatt 13560 cttcttgtca tcttgttcag aatatacag caatgctata cctaatacta aatacattat 13620 catgctcgca atggctctaa caacgacgaa caaaatgaa tttggtcgta gcttttgttc 13680 aaaaatac ataagaat gtctacataa atctatggcg ccattggcta cttgaatag 13740 cgccagtcct cctacagatt ttatatagc tgtataacat gatattatt catcatcaa 13800 agagacagag tcaccatctg tcatatttag atttttttc atgtgttcaa agtatcctct 13860 actcattca tattatagt tatcatact tagatttta ggacggatca atgagtaaga 13920 cttgactaga tcgtcagtag taatttgtgc atcgtctatt ctgcatccgc ttcgtcgaat 13980 aatgtatagc atcgctttga gattctccat agctatcaag tctttataca atgacatgga 14040 atatctgtg atactttat acttctccaa catcgatgcc ttacatcat cgcctacttt 14100 agcattgaaa atacgttcta ttgtgtagat ggatgtaaca agatttttaa acaacaatgc 14160 cattttacac gatgattgtc tcaagtctcc aatcgttgt ttagaacgat tagctacaga 14220 gtccaatgct tggctgacta gcatattatt atctttagaa attgtattct tcaatgaggc 14280 gtttatcata tctgtgatt cgttagtcat attackactct gactggttg taatgttatc 14340 siacatatca cctaggata cggtacacgt accaccattt gtaataatcc tatctagat 14400 gttgtatggc attgcgcaga aaatcttc tcctgtatata tctccactct cgataaatct 14460 actcagatta ttcttaatg ccttattctc tggagaaag atatcagtgt ccatcattc 14520 attatagta tacgcagaa agataccacg agtatcatt ctatchcaga tacttacgg 14580 ttccgagtca cagataatgg ttcctctcc ttcgggagat cctgcataga aatctagg 14640 acaatagttt ctatactgtc tgtaacctg ataatctcta aagtcactaa ctgataccat 14700 gaaattgaga agatcaacg ctgaagtaat taatttttct gcctcgtttt tactacaact 14760 agttttcatc aatgtagtga cgatgtattg tttagttact cttggtctaa tactgatgat 14820 agagatatta ttactccca taatggatct tctagtagtc accttaagc ccattgatgc 14880 aaatagcaga tagataagt cttggtatga ctccttttcta atagtacg gactaccttt 14940 gtcacccaac ttataccca cataagccat aacaacctct ttatagccg ttcatgagg 15000 tttatcagcc atgagcctga gtagttggaa gatctcatg aatcccgtct cagaaagtcc 15060 tatatgcatg atagatttat ctttcctggg aaactctcgt atagtcatag atgaaatact 15120 cttcaaagtt tctgaataa gattagtaac agtcttacct ccgactactc taggtacaa 15180 aaaactcta atagtgtt tctctgcgga gataatca gaaggatag agcaatagt 15240 agtattattg tgatttaaa gaccgaatac atacaggta gatttataa acatcatgtc 15300 ctgaaggttt ttagacttgt attcctcgta atccataccg tccaaaaca tggattttgt 15360 aactttgata gccgtagatc ttgttcctt cgccaacagg ttaagaaat taataagaa 15420 tttgtggttt ctacctatgt ctacaaattg cacgttttgga agcgtactg tcgttcac 15480 tgcagcattt tgaggatttc tgcagcattt tgttctg gtatatctgg 15540 aaagaatttct accagtctag gaatagaga ttgatatcgc atagaatac aaagttcat 15600 aatctcatca tcgaagagca tttgttacc attgtaataa atatccactc tgtcatatgt 15660 aaatgaag tactgttcaa acatgatgag atgtttatat gttggcatag tagtgagatc 15720 gacgttttggt aatggcaatg tattagatt aactccataa tgtctagcag catctgcgat 15780 gttataagcg tcgtcaaagc ggggtcgatc ttgtgctgtt atatattgtc taacacctat 15840 aagattatca aaatcttgtc tgcttaatac accgttaaca atttttgcct tgaatttcttt 15900 tattggtgca ttaacat ccttatagag gatgttaaac aaataagtat tatcaagtt 15960 aagatctggg tattctttt ctgctagaac atccattgag tcggagccat ctggtttaat 16020 ataaccaccg ataatctag ctctgtattc tgtatccgtc aatctaat tagaaggtg 16080 ttgagtgaaa ggtggagat cgtaaaagct gtgagtatta atataggt tagttttccga 16140 actaatgtta attggggtat taataatatc tatatttcca gcgttaagtg taacattaaa 16200 cagttttaat tcacgtgacg tggtatcaat taaatatta atgcccatt tggatatagc 16260 agcctgaagc tcatcttgtt tagttacgga tcctaatgag ttattaagca atatatcga 16320 cggatgaacg aaggttgtt taagttggtc acatactttg taatctagac atagatgcgg 16380 aagaacggta gaaactatac gaaataata ttcagagtcc tctattgat caagtaac 16440 tattgactta ataggcatca tttattagt attaaatgac gaccgtacca gtgacggata 16500 tacaaacga tttattaca gagttttcag agaatatta tccatactaac aaaaattatg 16560 aaatactct tcgtcaatg tctattctaa ctcacgttaa caacgtggta gatagagaac 16620 ataatgccgc cgtagtgtca tctccagagg aaatatcctc acacttaat gaagacttat 16680 ttccagatga tgatcaccg gccactatta tcgaacgagt actactatta 16740 ttgacgatac tccacctcct acgtttcgta gagagttatt gatatcggaa caacgtcaac 16800 aacgaaaa aagatttaat attacagtat cgaaaaatgc tgaagcaata atggaatcta 16860 gatctatgat aacttctatg caccacaaa caccatcctt gggagtagtt tatgataag 16920 aaaagaat tcagatgtta gaggatgaag tggttaatct tagaatca cgatctaata 16980 caaaatcatc tgataattta gataattta ccaaatact atttggtaag actccgtaca 17040 aatcacaga agttaatag cgtatagcca tcgttaatta tgcaatttg aacggctctc 17100 ccttatcagt cgaggacttg gatgtttgtt cagaggatga aatagataga atctataaaa 17160 cgattaaaca atatcacgaa agtagaaaac gaaaaattat cgtcactaac gtgattatta 17220 ttgtcataaa cattatcgag caggcattgc taaaactcgg atttgaagaa atcaaaggac 17280 tgagtaccga tatcacttca gaaattatcg atgtggagat cggagatgac tgcgatgctg 17340 tagcatctaa actaggaatc ggtaacagtc cggttcttaa tattgtattg tttatactca 17400 agatattcgt taaacgaatt aaaattattt aatttaatac attcccatat ccagacaaca 17460 atcgtctgga ttaatctgtt cctgtcgtct cataccggac gacatattaa tctttttatt 17520 agtgggcatc ttttagatg gtttcttttt cccagcatta actgattcga tacctagaag 17580 atcgtgattg atctctccga ccattccacg aacttctaat tggccgtctc tgacggtacc 17640 ataaactatt ttaccagcat tagtaacagc ttggacaatc tgaccatcca tcgcattgta 17700 cgatgtagta gtaactgttg ttctacgttt aggagcacca gaagtatttt tggagccctt 17760 ggatgttgat gtagaagaag acgaggattt tgattttggt ttacatgtaa tacattttga 17820 actctttgat ttgtatcac atgcgccggc agtcacatct gtttgagaat tagattatt 17880 gttgcctcct ttgacggctg catctccacc gatttgcgct agtagattt taagctgtgg 17940 tgtaatctta ttaactgttt cgatataatc atcgtaactg cttctaacgg ctaaattttt 18000 tttatccgcc atttagaagc taaaaatatt tttattg cagaagattt aactagatta 18060 tacaatgaac taatatgatc cttttccaga ttatttacaa acttggtatt tcttggttct 18120 ggaggaggcg aatttaatt cggacttgga ttcggatttt gtggttctt gatcttatta 18180 tacagcgcat ataggatggc gacggtact gctacgcaa taccgatca caaagaata 18240 ccaatcatttt attgacaata acttcactat tgatcagta tgcaatat catcttttca 18300 ctaaatagt agtaataatg attcaacaat gtcgagatat atggacgata ataacttagt 18360 tcacggaaat atcgctatga tggtgtgaa tgactccgct aactctgtgg ggcgcgcagt 18420 gctttcccca catagaataa attagcattc cgactgtgat ataatacca agtataaacg 18480 ccataatact caatactttc catgtacgag tgggactggt agactacta aagtcaataa 18540 aggcgaagat accgaaga atcaaagaa tgattccagc gattagcacg ccggaaaaat 18600 aatttccaat catagcatc atgtccattt actaataa aattttaaat cgccgaatga 18660 aaagtgga ataaacca tataaaaaca atagtttgta ctgcaaaaat atatctatt 18720 tttgttttcg aagatatggt aaattaaat agtagtacac agcatgttat actacagc 18780 agcaacggct cgtaattact tatcatttac tattttctg ggtggtggga 18840 ctcaaataat acgaatatat cacccatcca ttttatgcga tgtttatata ctctaatctt 18900 taatagatct atagacgacg ggtttaccaa caatagat tttacgatt catctaattt 18960 aaacccttcc ttaaacgtga atgatctatt atctggcata acgatgactc tacctgatga 19020 atcggacaat gtactgggcc atgtagaata attatcac gatttacgt ctacgaacat 19080 ttatatcatt tgttttaatt ttaggacgcg aaaatgga tataaaatag aaatacag 19140 atattacaac cagtgttatg gccgcgccca accaggtagg cagttttatt ttatcttta 19200 ctacaggttc tcctggatga tgtacgttac caactgcaga cgtagttcta gtacaattag 19260 acgtaagttc cgcttgggaa ttttaacg ctaaagagtt aacgttaatc gtgcacccaa 19320 cgtatttaca tctagttctt tgaacatct gattataata taaccatttt ctactctctag 19380 attcgtcggt gcactcatgt aaccaacata ccctaggtcc taatatta tctccggaat 19440 tagattttgg atattcggg caccaacaat ttctatttcc tttatgatcg ttacaaaga 19500 cgtataatgc cgtatcccca aaagtaaat atcaggacg ataattcta ataactcag 19560 aacaatatct cgcatccata tgttggagc aaatatcgga atagtagac atagccggtt 19620 tccgttttgc acgtaaccat tctaaacaat tggggttcc aggatcgttt ctacaaaatc 19680 cagtcatgaa atcatcacaa tgttctgtct tgtatattatt attaaatatt tttggacagt 19740 gtttggtatt tgtcttagaa cacatttg ccacgctatc actacgccc aggataat 19800 cctttttat aaatgacat cgttgcccgg atgctatata atcagtagcg tgttttaaat 19860 ccttaatata ttcaggagtt acctcgttct gataatagat taatgatcca ggacgaaatt 19920 tgaaagaact acatggttct ccatgaatta atacatattg tttagcaaat tcaggaacta 19980 taaaactact acaatgatct atcgacatac catctca acaaatttg ggtttaattt 20040 ctcctggaga cgttcataa taatacatat aactttctc ggcaaactta acagctctat 20100 tatattcagg ataattaaaa cctaattcca tatattgtc tcgtatatct gctattccctg 20160 gtgctatttt aattctatta agagtaacgg ctgccccat ttttaataat cgtcagtatt 20220 taaactgtta aatgttggta tatcacatt taccttattt cccgcagtat aaggttttgtt 20280 gcaggtatac tgttcaggaa tggttacatt tatactctt ctatagtcct gtctttcgat 20340 gttcatcaca tatgcaag acagaataa caaataatg windows tattaatat 20400 ctgtgaatttc gtaattacat tgattgccat atattaca gcagctacaa tacatacaat 20460 agacattccc acagtgttgc cattacctcc acgatacatt tgagttacta agcaataggt 20520 ataactaag ctagtaagg gcaatagaa agatgagata atacatca atagagat 20580 tagaggaggg ctatatagag ccaagacgaa caaaatcaaa ccgagtaacg ttctaacatc 20640 attatttttg aagattccca aataatcatt cattcctcca taatcgtttt gcatcatacc 20700 tccatcttta ggcataaacg attgctgctg ttcctctgta aataaatctt tatcaagcac 20760 tccagcaccc gcagagaagt cgtcaagcat attgtaatat cttaaataac tcatttatat 20820 attaaaaaat gtcactatta aagatggagt ataatcttta tgccgaacta aaaaaaatga 20880 cttgtggtca acccctaagt ctttttaacg aagacgggga ttttgtagaa gttgaaccgg 20940 gatcatcctt taagtttctg atacctaagg gattttacgc ctctccttcc gtaaagacga 21000 gtctagtatt tgaaacatta acaacgaccg ataataaaat cactagtatc aatccaacaa 21060 atgcgccaaa gttatatcct cttcaacgca aagtcgtatc tgaagtagtt tctaatatga 21120 ggaaaatgat cgaatcaaaa cgtcctctat acattactct tcacttggcg tgtggatttg 21180 gtaagactat taccacgtgt tatcttatgg ctacacacgg tagaaaaacc gtcatttgcg 21240 tacccataa aatgttaata catcaatgga agacacaggt agaggcagtc ggattggaac 21300 atagatatc catagatgga gtaagtagtc tattaagga actaagact CAagtccgg 21360 atgtattaat agtagtcagt agacatctga cieacgatgc cttttgtaaa tatatcaata 21420 agcattatga ttgttcatc ttggatgaat cacatacgta taatctgatg aacaatacag 21480 cagttacaag atttttagcg tattatccctc cgatgatgtg ttatttta actgctacac 21540 ctagaccatc taacagaatt tattgtaaca gtattatta tattgccaag ttatccgatc 21600 taaaaaaaac tatctatgcg gtagatagtt tttttgagcc atattccaca gataatta 21660 gaatattggt aaacgacta gatggaccat ctaataata tcatatat accgagagt 21720 tattatctgt agacgagcct agaatcaac tattacttta taccctgta gagaattca 21780 agtcaggaac tattaatcgc attttagtta tattaactaact acgtgaacat atggtattat 21840 tctacaaacg attattagat cttttcggac cagaggttgt atttagga gacgcccaaa 21900 atagacgtac tccagatatg gtcaatcaa tcaggaac aaatagattt atattcgtat 21960 ccaccttatt ttattccggt actgtttag atattcctag tttggattct ttgttcattt 22020 gctcggcagt atcaacaat atgcaatag agcaattact agggatta tgtcgagaaa 22080 cagaactatt agataggacg gtatatgtat ttcctagcac atccatcaa gaataaagt 22140 acatgatagg aaatttcgtt caacgaatta ttagtctgtc tgtagataaa ctaggattta 22200 aacaaaaaag ttcggaaa catcagaat ccgatcccac ttctgcatgt acacatcat 22260 ccagagaga acgtgttatta atagaata ttactcgca aaatcgttaa gaagtttaag 22320 cgacgatccg catgctgcgc aggccagtgt attaccccctc atagtattaa tataatccaa 22380 tgatacttt gtgatgtcgg aaatcttaac caatttagac tgacaggcag aacacgtcat 22440 gcaatcatca tcgtcatcga taactgtagt cttgggctc ttttgcgac tcttcattcc 22500 ggaacgcaca ttggtgctat ccatttaggt agtaaaaaat aagtcagaat atgccctata 22560 gcacgatcgt gcaaaacctg gtatatcgtc tctatcttta tcacaata gtgtatcgac 22620 atttttatta ttattgacct cgtttatctt ggaacatgga atggaaacat tttgttatc 22680 aacggccacc tttgccttaa ttccagatgt tgtaaatta taactaaca gtctatcatc 22740 gabacaatg aaattcttgt ttagacgtttt gtagtttacg tatgcggctc gttcgcgtct 22800 cattttttca gatttgcag gtactataat atttaaaata agaatgaat aacataggat 22860 taaaaaaaa gttatcatga cttctagcgc tgatttaac aacttaaaag attack 22920 tctgtacaa agtttgagat ttcagattc tgcggctata gaaagtata attctttggt 22980 agaatgggga acatctactt actggaaat aggcgtgcaa aaggtagcta atgtcgagac 23040 gtcaatatct gatttattag atgaggtaaaaataaccg tttaatattg atccggggcta 23100 ttacattttc ttaccggtat attttggg cgtctttatt tattcgagg gtaaaaatat 23160 ggtagaactt ggatctggaa actctttca ataccagat atatgcgaa gtgcgtgtaa 23220 caagtatta gatagtgata acggaataga ctttctgaga ttgttttgt windows 23280 atggataatg gaatgcta tatcaaata tcagtctcca gttaatat ttaaactagc 23340 tagtgagtac ggattaaca taccaata tttagaatt gaatagagg aagacacatt 23400 atttgacgac gagttatact ctattataga acgctcttt gatgataaat ttccaaaat 23460 atccatatcg tatattagt tgggagaact taggcggcaa gttgtagact tttcaaatt 23520 ctcattcatg tatattgagt ccatcaggt agatcgtata ggagataata ttttattcc 23580 tagcgttatta acaaatcag gaaaaaagat attagtaaa gatgtagacc attackaacg 23640 atccaaggtt agagacata cattgtaaa agtaaaaag aaaaacacat ttccatttt 23700 atacgactt gatgaacg gagagaac gatgagaa gtaaaac gatttaga 23760 cactatagga cgagactatt atgttaacgg aaagtatttc tctaggttg gtagtgcagg 23820 cttaaagcaa ttgactaata attagat taatgagtgc gcaactgtcg atgagttagt 23880 tgatgagatt aaaatccg gaactgtaa acgaaaata aaaaaccaat cagcatttga 23940 tttaagcaga gatgttttgg gatatccaga agcggatttt ataacgttag ttaatacat 24000 gcggttcaaa atagaaatt gtaaggttgt aaatttcaat attgaaaata ctaattgttt 24060 aaataacccg agtattgaaa ctatatatgg aaactttaac cagttcgtct caatctttaa 24120 tgtcgtcacc gatgtcaaaa aaagattatt cgagtgaaat aatatgcgcc tttgatatag 24180 gtgcaaaaaa tcctgccaga actgttttag aagtcaagga taactccgtt agggtattgg 24240 atatatcaaa attagactgg agttctgatt gggaaaggcg catagctaaa gatttgtcac 24300 aatatgaata cactacagtt cttctagaac gtcagcctag aaggtcgccg tatgttaaat 24360 ttatctattt tattaaaggc tttttatatc atacatcggc tgccaaagtt atttgcgtct 24420 cgcctgtcat gtctggtaat tcatagagag atcgaaaaaa gagatcggtc gaagcatttc 24480 ttgattggat ggacacattc ggattgcgag actccgttcc ggatagacgc aaattagacg 24540 atgtagcgga tagtttcaat ttggctatga gatacgtatt agataaatgg aatactaatt 24600 atacacctta taataggtgt aaatctagaa attacataaaa aaaaatgtaa taatagggat 24660 aacagggtaa t 24671 <210> 7 <211> 25970 <212> DNA <213> Vaccinia virus <400> 7 tagggataac agggtaattt aggtagtaaa aaataagtca gaatatgccc tatagcacga 60 tcgtgcaaaa cctggtatat cgtctctatc tttatcacaa tatagtgtat cgacattttt 120 attattattg acctcgttta tcttggaaca tggaatggaa acatttttgt tatcaacggc 180 cacctttgcc ttaattccag atgttgtaaa attataacta aacagtctat catcgacaca 240 aatgaaattc ttgtttagac gtttgtagtt tacgtatgcg gctcgttcgc gtctcatttt 300 ttcagatatt gcaggtacta taatattaaa aataagaatg aaataacata ggattaaaaa 360 taaagttatc atgacttcta gcgctgattt aactaactta aaagaattac ttagtctgta 420 caaaagtttg agattttcag attctgcggc tatagaaaag tataattctt tggtagaatg 480 gggaacatct acttactgga aaataggcgt gcaaaaggta gctaatgtcg agacgtcaat 540 atctgattat tatgatgagg taaaaaataa accgtttaat attgatccgg gctattacat 600 tttcttaccg gtatattttg ggagcgtctt tatttattcg aagggtaaaa atatggtaga 660 acttggatct ggaaacctt ttcaatacc agatatatg cgaagtgcgt gtaacaaagt 720 attagagt gataacggaa tagactttct gagatttgtt ttgttaaca atagatggat 780 aatggagat gctatatca attackcagtc tccagttaat atttaac tagctagtga 840 gtacggatta aacataccca atatttaga attgaata gaggagaca cattatttga 900 cgacgagtta tactctatta tagacgctc ttttgatgat aaatttccaa aaatatccat 960 atcgtatatt aagttgggag aacttaggcg gcaagttgta gactttttca attctcatt 1020 catgtatatt gagtccatca aggtagatcg tataggagat atatttt ttcctagcgt 1080 tataacaaaa tcaggaaaaa agatagt aaagatgta gaccatta tacgatccaa 1140 ggttagagaa catacatttg taaaagtaaa aaagaaaaac acatttcca ttttatacga 1200 ctatgatgga aacggaacag aaactagagg agagtaata aaacgaatta tagacactat 1260 aggacgagac tattatgtta acggaagta ttctctaag gttggtagtg caggcttaaa 1320 gcaattgact ataaattag atattag gtgcgcaact gtcgatgagt tagttgact 1380 gatttaaaa tccggaactg taaaacgaaaaaaaaac caatcagcat ttgatttaag 1440 cagagaatgt ttgggatatc cagaagcgga ttttaacg ttagttaata acatgcggtt 1500 caaatagaa attgtagg ttgtaattt caatattgaa atactatt gtttaataa 1560 cccgagtatt gaactatat atggaactt taaccagttc gtctcaatct ttaatgtcgt 1620 caccgatgtc aaaaaaagat tattcgagtg aaatatatg cgccttttgat ataggtgcaa 1680 aaaatcctgc cagaactgtt ttagaagtca aggatactc cgttaggta ttggatatat 1740 caaattaga ctggattct gattgggaaa ggcgcatagc windowatttg tcacaatg 1800 atacactac agttctctta gaacgtcagc ctagaaggtc gccgtagtt aaatttatct 1860 attttattaa aggcttttta tatcatacat cggctgccaa agttattgc gtctcgccctg 1920 tcatgtctgg taattcatat agagatcgaa aaagagatc ggtcgaagca tttcttgatt 1980 ggatggacac attcggattg cgagactccg ttccggatag acgcaaatta gacgatgtag 2040 cggatagttt caatttggct atgagatacg tattagataa atggaatact aattatacac 2100 cttataatag gtgtaaatct agaaattaca taaaaaaaat gtaataacgt tagtaacgcc 2160 attatggata atctatttac ctttctacat gaatagaag atagatatgc cagaactatt 2220 tttaactttc atctaataag ttgcgatgaa attaggata tatatggtct tatgaaagaa 2280 cgcatttcct cagaggatat gtttgatat atagtgtata ataagatat acatcatgcc 2340 attagaaac tagtgtattg cgacatccaa cttactaac acattattaa tcagaatacg 2400 tatccggtat ttaacgattc ttcacaagtg aaatgttgtc attatttcga tataaactca 2460 gataatagca atattagctc tcgtacagta gagatatttg agaggaaaa gtcatctctt 2520 gtatcatata ttaaaactac caataagaag agaaggtca atttagggga aaaagaa 2580 actgtacatg gaggcactaa tgcaattac tttccggta aaagtctga tgagtatctg 2640 agcactacag tcaggtccaa cattaatcaa ccttggatca aaaccattc taagagaatg 2700 agagtagata tcattaatca ctctatagta acgcgtggaa aaagctctat attackaact 2760 atagaaatta ttttacta tagacatgt gtgaaaatat tcaggattc tactatgcac 2820 attattctat ccaaggacaa ggatgaaaag gggtgtatac acatgattga caaattattc 2940. 2940. 2940. 2940. 2940. 2940. 2940. 2940. 2940 gaagtagcta atgttgtaaa ccacgtactc acggctacgg cattagga gaattattc ctaattaaga aaatggctga acacgatgtt tatggagtta gcaatttcaa aatagggatg tttaacctga catttattaa gtcgttggat cataccgttt tcccctctct gttagatgag gatagcaaaa taagttttt tagggaaa aagctcaata ttgtagcatt acgatctctg gaggattgta taattacgt gactaaatcc gagaattga tagaaatgat gaggaaaga tcgactattt taatagcat agatatagaa acggaatcgg tagatcgtct aaaagaattg cttctaaaat gaaaaaaaac actgattcag aaatggatca acgactaggg tataagtttt tggtgcctga tcctaaagcc ggagtttttt atagaccgtt acatttccaa tatgtatcgt attctaattt tatattgcat cgattgcatg aaatcttgac cgtcaagcgg ccactcttat cgtttaagaa taatacagaa cgaattatga tagattag caatgttaa gtgactcctc cagattactc acctataatc gcgagtatta aaggtaagag ttatgacgca ttagccacgt 3600 tcactgtaaa tatctttaaa gaggtaatga ccaaagaggg tatatccatc actaaaataa 3660 gtagttatga gggaaaagat tctcatttga taaaaattcc gctactaata ggatacggga 3720 ataaaaatcc acttgataca gccaagtatc ttgttcctaa tgtcataggt ggagtcttta 3780 tcaataaaca atctgtcgaa aaagtaggaa ttaatctagt agaaaagatt acaacatggc 3840 caaaatttag ggttgttaag caaactcat tcactttctc gttttcctc gtatcccctc 3900 ctaatgtatt accgacaaga tatcgccatt acaagatatc tctggatata tcacaattgg 3960 aagcgttgaa tatatcatcg acaaagacat ttataacggt caatattgtt ttgctgtctc 4020 aatatttatc tagagtgagt ctagaattca ttagacgtag tttatcatac gatatgcctc 4080 cagaagttgt ctatctagta aacgcgataa tagatagtgc taaacgaatt actgaatcta 4140 ttactgactt tatattgat acatacatta atgacctggt ggaagctgaa cacattaaac 4200 aaaaatctca gttaacgatt aacgagttca aatatgaaat gctgcataac tttttacctc 4260 atatgaacta tacacccgat caactaaagg gattttatat gatatcttta ctaagaaagt 4320 ttctctactg tatctaccac acttctagat atccagatag agattcgatg gtttgtcatc 4380 gtatactaac atacggcaaa tattttgaga cgttggcaca tgatgaatta gagaattaca 4440 taggcaacat ccgaaacgat atcatgaaca atcacaagaa cagaggcact tacgcggtaa 4500 acattcatgt actaacaact cctggactta atcatgcgtt ttctagctta ttgagtggaa 4560 agttcaaaaa gtcagacggt agttatcgaa cacatcctca ctattcatgg atgcagaata 4620 tttctattcc taggagtgtt ggattttatc cggatcaagt aaagatttca aagatgttt 4680 ctgtcagaaa atacatcca agtcaatatc tttacttttg ttcatcggac gttccggaaa 4740 gaggtcctca ggtaggttta gtatctcaat tgtctgtctt gagttccatt acaaatatac 4800 taacgtctga gtatttggat ttggaaaaga aaatttgtga gtatatcaga tcatattata 4860 aagatgatat aagttacttt gaaacaggat ttccaatcac tatagaaaat gctctagtcg 4920 catctcttaa tccaaatatg atatgtgatt ttgtaactga ctttagacgt agaaaacgga 4980 tgggattctt cggtaacttg gaggtaggta ttactttagt tagggatcac atgaatgaaa 5040 ttcgcattaa tattggagcg ggaagattag tcagaccatt cttggttgtg gataacggag 5100 agctcatgat ggatgtgtgt ccggagttag aaagcagatt agacgatatg acattctctg 5160 5220 5280 acgatttatg tgactttcct gccgaattta gagatggata tgtagcatct tcactagtgg 5340 gaatcaatca caattctgga cccagagcta ttcttggatg tgctcaagct aaacaagcta 5400 tctcttgtct gagttcggat atacgaaata aaatagacaa tggaattcat ttgatgtatc 5460 cagagaggcc aattgtcatt agtaaggctt tagaaacttc aaagattgcg gctaattgct 5520 tcggccaaca tgttactata gcattaatgt cgtacaaagg tatcaatcaa gaggatggaa 5580 ttatcatcaa aaaacaattt attcagagag gcggtctgga tatagttacc gcaaagaaac 5640 atcaagtaga aattccgttg gaaacttta ataacaaaga aagagatagg tctaacgcct 5700 attcaaatt agaaagtaat ggattagtta gactgaatgc ttcttggaa tccggagacg 5760 ctatggcacg aaatatctca tcagaactc ttgaagattga tttgctaga gataatcaga 5820 ttagcttcga tgtttccgag aatataccg atatgtacaa atctcgcgtt gaacgagtac 5880 aagtagaact tactgacaa gttaaggtac gagtattac catgaaga agagaccca 5940 ttctaggaga caaatttacc actagaacga gtcaaaggg aacagtcgcg ttgtcgcgg 6000 atgaaacgga acttccatac gacgaaaatg gtatcacacc agatgtcatt atttattcta 6060 catccatctt cttagaaaa actatatcta tgttgataga agttatttta acagccgcat 6120 attctgctaa gccgtacaac aaaaccgacc tgtctgtttt cctagtagta 6180 acgaaacatc catcgataca tatatgcaat tcgctaaca atgttatgag cattcaatc 6240 cgaaattgtc tgatgaagaa ttatcggata aaatctttg tgaaagatt ctctatgatc 6300 ctgaaacgga taagccttat gcatccaag tattttgg accaatttat tacttgcgtc 6360 tgagacatttt aactcaggac aaggcaccg ttagatgtag agtaaaag acgaagctca 6420 ttagacaagc gaatgaggga cgaaaacgtg gaggaggtat taagtttgga gaaatggaga 6480 gagactgttt atagcgcat ggcgcagcca atactattac agaagttttg aaagattcgg 6540 aagaagatta tcaagatgtg tatgtttgtg aaaattgtgg agacatagca gcacaaatca 6600 agggtattaa tacatgtctt agatgttcaa aacttaatct ctctcctctc ttaacaaaaa 6660 ttgataccac gcacgtatct aaagtatttc ttactcaaat gaacgccaga ggcgtaaaag 6720 ttaaattaga ttcgaacga aggcctcctt cgttttataa accattagat aaagttgatc 6780 tcaagccgtc ttttctggtg tatattcta gtttggtagt agatacatat caatatcatc 6840 aaattcgaga tccgaattat aaaatgggcg tggattgtta actatagaat cggacgtctg 6900 atattcgaaa atctgtggag tttcaggtttt tggtggaggt gtaactgcta cttgggatac 6960 tgaagtctga tattcagaaa gctgggggat gttctggttc gacatccacc gatggtgtca 7020 catcactaat cggttcggta acgtctgtgg atggaggtgc tacttctaca gaacctgtag 7080 cctcagttgt caacggagat acatttttaa tgcgaggaaa tgtataattt ggtaatggtt 7140 tctcatgtgg atctgaagaa gaggtaagat atctactaga aagataccga tcacgttcta 7200 gttctctttt gtagaactta actttttctt tctcagcatc tagttgatat tccaacctct 7260 tcacgttact acgttcagat ttcaattcac gttcgcatgg gttacctccg cagtttttac 7320 gagcgatttc acgttcagcc ttcatgcgtc tttccctctc tctatcgagt ttatcagagc 7380 agtctttctg aaggcgatcg aactccataa atttctccaa cgctttgatt gtttccatag 7440 atttccgaag ttcagctttt aggactgtga ttctttttct ttcgaattca cagctggatg 7500 tacaaccgtt tccattaccg ccatctctaa gtttcttttc tagatcggca acatttcatc 7560 cccatgcctt ttacattcct cgagtctact gtcgtcgaaa tatcgttcca gctccttttc 7620 gacatcaata actttagcac gttgtctctc aagctctctt ttgtagttat ctgattccct 7680 ggcacgttta agatcttcat gcaattgagt cagctcttaa cacaatctct tgcttcttcg 7740 tcatagtact tacaatcact atgggatcca ttgttaccac gtctacactc ggcgagctcg 7800 cgtttaagag attcaatttc ccgtttgtat tggtccatgt ttccattgct accaccatta 7860 gatttcagg ctgctagttg tcgttcgaga tcagaaatac gggttttctt ggaattgatt 7920 tcgtcgatgt acttggcatc gaaacactta ttaagttctt tttccaattc tacgatttta 7980 tttctttcgc gagtcaattc cctcctgtag taactatctg ttttgtcaga ttcacgctct 8040 ctacgtagac tttcttgcaa gttactaatt tgttccctag cacgtccgag tttagtttta 8100 tatgccgaat agagttctga ttcatccttt gagcagatct ctagagatcg ttcaagatcc 8160 ctgattctag tctttagcct atttacctcc tcggaagaatg ctccgttacc gttgcgttta 8220 cactcgttaa gctgtctatc aagatccatg attctatctc taagacgttg catctctctt 8280 tccatatcag cattgctttc attattacgt ctgcagtcac tcaactgtct ttcaatatct 8340 gagattctat ctctaaagacg tcgcatctct ctctgtttcg gcattggttt cattattacg 8400 tctacagtcg ttcaactgtc tttcaagatc tgatattcta gattggagtc tgctaatctc 8460 tgtagcattt tcacggcatt cactcagttg tctttcaaga tctgagattt tagattggag 8520 tctgctaatc tctgtaagat ttcctctcc gctctcgatg cagtcggtca acttattctc 8580 tagttctcta atacgcgaac gcagtgcatc aacttctttgc gtgtcttcct ggttgcgtgt 8640 acattcatcg agatctctaa cgcgtcgtcg ttcttcctca agttctctgc gtactacaga 8700 aagcgtgtcc ctatcttgtt gatatttagc aatttctgat tctagagtac tgatttgct 8760 tacgtagtta ctaatattg tcttggcctt atcaagatcc tccttgtatt tgtcgcattc 8820 cttgatatcc ctacgaagtc tggacagttc ccattcgaca ttacgacgtt tatcgatttc 8880 agctcggaga tcgtcatcgc gttgttttag ccacatacga ctgagttcaa gttctcgttg 8940 acaagatcca tctacttttc cattcctaat agtatccagt tccttttcta gttctgaacg 9000 cattctcgt tccctatcaa gcgattctt caattctcgg atagtcttct tatcaatttc 9060 tataaatct gaaccatcat ctgtcccatt ttgaatatcc ctgtgttctt tgatctcttt 9120 tgtaagtcgg tcgattcttt cggttttata aacagaatcc ctttccaaag tcctaatctt 9180 actgagttta tcactaagtt ctgcattcaa ttcggtgagt tttctcttgg cttcttccaa 9240 ctctgtttta aactctccac tatttccgca ttcttcctcg catttatcta accattcaat 9300 tagtttatta ataactagtt ggtaatcagc gattcctata gccgttctg taattgtggg 9360 aacataatta ggatctcta atgattgta tggcttgata gcatcatctt tatcattatt 9420 aggggatgg acaaccttaa ttggttggtc ctcatctcct ccagtagcgt gtggttcttc 9480 ataccagtg ttagtaatag gcttaggcaa atgcttgtcg tacgcgggca cttcctcatc 9540 catcaagtat ttataatcgg gttctacttc agaatattct ttctaagag acgcgacttc 9600 gggagttagt agagaactc tgtttctgta tctatcaacg ctggaatca tactcaagtt 9660 aaggatagcg atacctcat cgtcatcatc cgtatctctct gaacaccat catatgacat 9720 ttcatgaagt ctaacgtatt gataaacaga atcagattta gtattaaca gatccttaac 9780 ctttttagta aacgcatatg tatattttag atctccagat ttcatatatat gatcacatgc 9840 cttaaatgtc agtgcttcca tgatataatc tggaacacta atgggtgacg aaaaagatac 9900 agcaccatat gctacgttga taaataaatc tgaaccacta agtagataat gattaatgtt 9960 aaggaaaaga aaatattcag tgtataggta tgtcttggcg tcatatcttg tactaacac 10020 gctaacagt ttattgt gatcaatttc spiagaata attagagcag caggaatacc 10080 aaaaacata ttaccacatc cgtattttct atgaatca catacatgt taaaaaatct 10140 tgatagaga gcgaatct cgtctgactt aatgagtcgt agttcagcag cacatagt 10200 cataactgta atagaacat actttcctgt agtgttgatt ctagactcca catcacacc 10260 attattaaaa atagttttat atacatcttt aatctgctct ccgttaatcg tcgaacgttc 10320 tagtatacgg aacactttg atttcttatc tgtagttaat gacttagtga tatcacgaag 10380 attackacga attackatttc ttgttttct tgacagacct gattcagac tcaacatc 10440 gttccatagt tttttacct cagtggcgaa atctttggag tgcttggtac atttttcaat 10500 aaggttcgtg acctccattt attataaaaa attttattca aaacttaact acatcgggt 10560 aattataaaa tcgtagatct cccatgtggt ggaatactac catctatcgc atgtggatgg 10620 acagtaggta atggccatgg gaacagtaat gtttgcatat ttatctttct tgccagtatt 10680 actgcatatt gtcccaatgt ttcgatgtga tgttctacc tatcaactgc cgctgtatca 10740 caacaatagt gtccgatgaa attaagatta tgatccaatg tgtttaatat atgattatca 10800 agtcttatac gatccgcgtc ttttttgaca ggatcaggtt cttctacagg aagaagtttc 10860 ggcctcttat gatattcatg tctgggaaac ggtggtctag ggtgaggctc cggtatcgga 10920 gtgggttttg gattataatc atcatcgtct atgacatcat cttcgacttc gatatttatt 10980 ttgctatctt gatgatgtcc tgtatcagtt gcattttcag cactcgactg aattagcg 11040 cattcattgt ctattattac catatttcta aatccaaaat gtatatgttg aacatcacta 11100 ctatcgttga tgagtcttat agcatgaatt cgcttatcgt tatcgggttt agcttctgtc 11160 accttagcaa ttcctttttt attaaactct acataatcat atccatttct attgtttgtt 11220 ctaatataaa cgagtatagc atcattgcta aatttttcaa tagtatcgaa aacagaatat 11280 cctaaaccat ataatatata ttcaggaaca ctcaaactaa atgtccagga ttctcctaaa 11340 tacgtaaact ttaatagtgc gaaatcattc aaaaatctac cacttataga tagatagtac 11400 ataaatgcgt atagtagtct acctatctct ttattatgaa aaccggcatt acgatcatat 11460 atatcatgat atacctgtga cccgtttacg ttaaaccata aatacatggg tgatcctata 11520 aacatgaatt tatttctaat tctcagagct atagttaatt gaccgtgtaa tatttgctta 11580 catgcatact tgatacgctc attaataaga tttttatcat tgctcgttat ctcagaatcg 11640 tatatataag gagtaccatc gtgattctta ccagatatta tacaaaatac tatatataaa 11700 atatattgac caacgttagt aatcatataa atgtttaacg ttttaaattt tgtattcaat 11760 11820 gttagccatt gcattggggc tcctaatgga gattttttat tctcatccat tttaggatag 11880 gctttcataa agtccctaat aacttcgtga ataatgtttc tatgttttct actgatgcat 11940 gtatttgctt cgattttttt atcccatgtt tcatctatca tagatttaaa cgcagtaatg 12000 ctcgcaacat taacatcttg aaccgttggt acaattccgt tccataaatt tataatgttc 12060 gccatttata taactcattt tttgaatata cttttaatta acaaaagagt taagttactc 12120 atatggacgc cgtccagtct gaacatcaat ctttttagcc agagatatca tagccgctct 12180 tagagttca gcgtgatttt ccaacctaaa tagaacttca tcgttgcgtt tacaacactt 12240 ttctatttgt tcaaactttg ttgttacatt agtaatcttt ttttccaaat tagttagccg 12300 ttgtttgaga gtttcctcat tgtcgtcttc atcggcttta acaattgctt cgcgtttagc 12360 ctctggcttt ttagcagcct ttgtagaaaa aaattcagtt gctggaattg caagatcgtc 12420 atctccgggg aaaagagtc cgtccattta aagtacagat tttagaaact gacactctgc 12480 gttatttata tttggtacaa cacatggatt ataaatatcg atgttaataa catcagaaaa 12540 tgtaaagtct atacattgtt gcatcgtgtt aaatttcta atggatctag tattattggg 12600 tccaacttct gcctgaaatc caaatatgga agcggataca aaaccgtttc ctggataaac 12660 cacacatctc cacttttgct ttacatcaga aattgtgtcg ttgacatctt gaactctcct 12720 atctaatgcc ggtgttccac ctatagattt tgaatattcg aatgctgcat gagtagcatt 12780 aaatttcctta atattgccat aattttcata tattgagtaa ccctggataa aaagtaaca 12840 caccgcagcc gtcgctacca caataaaaaa aattgataga gagttcattt ataatctatt 12900 agaagctgac aaaatttttt tacacccatc agacaatgct ttaata gttcacatc 12960 tacttttgtc atatcgaacc gatggtatga ttctaccta gatttacatc cgaaaaagtt 13020 gactatgttc atagtcatta agtcattac aaaacatt ccagactctg gattataga 13080 cgatactgtt tcgtcacaat tacctacctt atcatgtga ttatgaatat tgctattag 13140 agcaccttct aagaaatcta taatacttt gaacacgat ttaaaatca accacgaata 13200 tactttacg aagaaagtta gtttacccat aggagata actataaatg gagatctaga 13260 tacaaaatcc ggatctatga tagttttaac attattat tctctattaa atacctccac 13320 atctaaaaat gttaatttg aaactagtc ttcgtttt accgtaccctg aactaaacgc 13380 tataagctct attgtttgag aactctttaa acgatattct tgaaatacat gtaacaaagt 13440 ttcctttaac tcggtcggtt tatctaccat agttacagaa tttgtatcct tatctataat 13500 ataataatca aaatcgtata aagttatata attatcgcgt tcagattggg atcttttcaa 13560 atagactaaa aaccccattt ctctagtaag tatcttatgt atatgttgt aaataatctt 13620 catggtggga atatgctcta ccgcagttat ccattcctca ttgacagcgg tagatgtatt 13680 agacaaaact attccaatgt ttaacaaggg ccattttacg agattattaa atccttgttt 13740 gataaatgta gccaatgagg gttcgagttc aacgacgatt gattctct cccgcggatg 13800 ctgcatgatg aacgacggga tgttgttcga ttgatttgga attctttc gactttttgt 13860 ttatattaaa tattttaaaa tttatagcgg atagcaattc atgtaccacg gatagtag 13920 acgcgtattg cgcatcgata tctttat tagataatt tatcataaa tgtgagaagt 13980 ttgcctcgtt aaggtcttcc atttaaatat tatataaca ttgtgtttg tatcttattc 14040 gtcttttatg gatagtttt ttactagtaa agctgcaatt accactttg tccgtaaaac 14100 aaataata acaccagctt ttcaatcg ttccaaaag tcgacggcgg acatttttaa 14160 catggcatct attttaata cacttaggtt ttggaaaa acatcatttt atattgtaa 14220 cgattcaata actaagaaa agatagat taacataag ggaatgtcat ttgtatttta 14280 taagccaaag cattctaccg ttgttaaata cttgtctgga ggaggtatat atcatgatga 14340 ttggttgta tgggagg taacattaa tgatctaag tgatgctat tttacatgga 14400 tttatcatat catggagtga caagtagtgg agcaatttac aaattgggat cgtctatcga 14460 tagactttct ctaaatagga ctattgttac aaagttaat attatgatg atacatttt 14520 tgacgacgat gattgatcgc tattgcacaa tttgttttt gtactttcta atatagtgtt 14580 taggttcttt ttcatatgag atattgatt tactaaaata tctatgttta acttttgttc 14640 tataacgtcc ttcggcgg tatcggtaca tatacgtaat tcaccttcac aaatacgga 14700 gtcttcgata atatagcca atcgattatt ggatctagct gtctgtatca tattcacat 14760 gttatatata tccttcgtt tccctttac aggcatcgat cgtagcatat ttccgcgtc 14820 tgagatggaa atgttaaaac tacaaaatg cgtaatgtta gcccgtccta atattggtac 14880 gtgtctataa gtttggcata gtagaataat agacgtgttt aaatgccttc caaagtttaa 14940 gaattctatt agagtattgc attttgatag tttatcgcct acatcatcaa aaataagtaa 15000 aaagtgtgct gattttttat gattttgtgc gacagcaata cattttcta tgttactttt 15060 agttcgtatc agattatatt ctagagcttc ctgactacta acgaaattaa tatgatttgg 15120 ccaaatgtat ccatcataat ctgggttata aacgggtgta aacaagaata tatgtttata 15180 ttttttaact agtgtagaaa acagagatag taaatagata gtttttccag atccagatcc 15240 tcccgttaaa accatcttaa acggcatttt taataaattt tctcttgaaa attgtttttc 15300 ttggaaacaa ttcataatta tatttacagt tactaaatta atttgataat aaatcaaaat 15360 atggaaaact aaggtcgtta gtagggagga gaacaaagaa ggcacatcgt gacataaata 15420 acatttatta tcatgatgac accagaaaac gacgaagagc agacatctgt gttctccgct 15480 actgtttacg gagacaaaat tcagggaaag aataaacgca aacgcgtgat tggtctatgt 15540 attagaatat ctatggttat ttcactacta tctatgatta ccatgtccgc gtttctcata 15600 gtgcgcctaa atcaatgcat gtctgctaac gaggctgcta ttactgacgc cgctgttgcc 15660 gttgctgctg catcatctac tcatagaaag gttgcgcta gcactacaca atatgatcac 15720 aaagaaagct gtaatggttt atattaccag ggttcttgtt atatattaca ttcagactac 15780 cagttattct cggatgctaa agcaaattgc actgcggaat catcaacact acccaataaa 15840 tccgatgtct tgattacctg gctcattgat tatgttgagg atacatgggg atctgatggt 15900 aatccaatta caaaaactac atccgattat caagattctg atgtatcaca agaagttaga 15960 aagtattttt gtgttaaaac aatgaactaa tatttatttt tgtacattaa taaatgaaat 16020 cgcttaatag acaaactgta agtaggttta agaagttgtc ggtgccggcc gctataatga 16080 tgatactctc aaccattatt agtggcatag gaacatttct gcattacaaa gaagaactga 16140 tgcctagtgc ttgcgccaat ggatggatac aatacgataa acattgttat ttagatacta 16200 acattaaaat gtctacagat aatgcggttt atcagtgtcg taaattacga gccagattgc 16260 ctagaccgga tactagacat ctgagagtat tgtttagtat ttttattaaa gattattggg 16320 TAGTTAAAAAACCAT AAAATGTT TAGTATTTA TAGTATAAA GATATAGATA 16380 ttagtaattt aaaaatttt aaacaactaa acagtacgac ggatgctgaa gcgtgttata 16440 tatacaagtc tggaaaactg gttaaaacag tatgtaaag tactcaatct gtactatgtg 16500 ttaaaaaatt ctacaagtga caaaaaaa tgaattaata ataagtcgtt aacgtacgcc 16560 gccatggacg ccgcgtttgt tattactcca atgggtgtgt tgactataac agatacattg 16620 tatgatgatc tcgatatctc aatcatggac ttataggac catacattat aggtaacata 16680 aaaactgtcc aaatagatgt acgggatata aaatattccg acatgcaaaa atgctacttt 16740 agcttaagg gtaaaatagt tcctcaggat tctaatgatt tggctagatt cacatttat 16800 agcatttgtg ccgcatacag atcaaaaat accatcatca tagcatgcga ctatgatatc 16860 atgttagata tagagataa acatcagcca ttttatctat tcccatctat tgatgtttttt 16920 aacgctacaa tcatagaagc gtataacctg tatacagctg gagattatca tctaatcatc 16980 aatccttcag ataatctgaa atgaattg tcgtttaatt cttcattctg catatcagac 17040 ggcaatggat ggattatat tgatgggaa tgcaatagta attttttc aaaagttg 17100 taagtaat aaaaaaaaaattga actagtagta cgtatattga gcaatcagaa 17160 atgatgctgg tacctctt cacggtgacc gtagttgcgg gaacaatatt agtagttat 17220 atattatata tttgtagga aaagatacgt actgtctata atgacaataa aattatcatg 17280 aaaattta aaagaataa gagttctaat tccagcaat ctagtaatc aactgatagc 17340 gatcagact gggaggatca ctgtagtgct atggacaaa acatgacgt agatatatt 17400 tctaggaatg agatattgga cgatgatagc ttcgctgta gtttaatatg ggataacgaa 17460 tccaatgtta tggcgcctag cacagaacac atttacgata gtgttgctgg aagcacgctg 17520 ctaataata atgatcgtaa tgaacagact atttatcaga acactacagt agtattaat 17580 gaacggaga ctgttaaagt acttaatgaa gataccaac agaatcctaa ctattcatcc 17640 aatcctttcg taaattataa taaaaccagt atttgtagca agtcaatcc gtttattaca 17700 gaacttaca ataatttag tgagaatat ccgtttagac gagcacatag cgatgatt 17760 cttaataagc aagaacaaga tcatgaacac gatgatatag atcatcggt cgtatcattg 17820 gtgtgattag tttccttttt aaaattga agtaatattt agtattattg ctgccgtcac 17880 gttgtacaa tggatatt ccctgtattc ggcattcta aattagcaa ttttattgct 17940 aaatgact gtagatatta tatagataca gaacatcaa aaattatatc tgatgagatc 18000 atagacaga tggatgaac ggtacttctt accacatct taagcgtaga agttgtaaat 18060 gatatgaga tgtaccatct tattcctcat agattcga cgattatact ctgtattagt 18120 tctgtcggag gatgtgttat ctctatagat aatgacgtca atggcaaaa tattctaacc 18180 tttcccattg atcatgctgt aatcatatcc ccactgagta aatgtgtcgt atcatgcaag 18240 ggtcctacaa ccatattggt tgttaaagcg gatataccca gcaacgatt ggtaacatcg 18300 tttacaaacg acatactata tgtaaacaat ctgtcactga ttaattatt gccgttgtct 18360 gtattcatta tagacgagt taccgactat tggatagac acatatgcga tcagatattt 18420 gcgaataata agtggtattc cattatacc atcgacaata agcagttttcc tattccatca 18480 aactgtatag gtatgtccctc tgccaagtac ataattcta gcatcgagca agatacttta 18540 attackgttt gtaacctcga gcatccattc gacttagtat aaaaaaat gcagtcgtac 18600 aattctgtac ctatcagga acaatattg tacggtagaa ttgatatat aaatgagc 18660 attagtattt ctgtgtatta atagatttct agtatgggga tcattaatca tctctaatct 18720 ctaaatacct cataaaacga aaaaaaagct attatcaat actgtacgga atggattcat 18780 tctcttctct ttttatgaaa ctctgttgta tatctactga taaaactgga agcaaaaaat 18840 ctgaaaaaa gatatagaat aagatcagg attattatgga acacgattta tataaaata 18900 caatagttcc tggttcctct tccacgtcta ctagctcgtg gtattataca catgcctagt 18960 atagtctct ttgcgttgat ggaagcaga ctagaaataa caggctaaaa tgttcagaca 19020 ccataatagt tcccaaccca gataaca gagttccatc aacacattcc tttaactca 19080 atcccaaacc caaaccgtt aaatgtatc cggccaattg atagtagata atgaggtgta 19140 cagcacatga taatttacac agtaaccaaa atgaaacac tttagtatt atagaaata 19200 tagacggtaa tgtcatcatc aacaatccga taatatgcct gagagtaaac attgacggat 19260 aaaacaaaaa tgctccgcat aacttatca tggcaatac acaaccaat acttgtaga 19320 ttcctaaatt agtagaaat acacggata tcgatgtata agtgatctcg agaataata 19380 agaataagt atgcccgta aagataaca tcacattgt ttggtaatca ttaaaccaat 19440 tagtatgaag ttgaactaat ttcacagtag atttattcc agtattatcc ccgcatgtat 19500 acgtacctgg tagatatct ttatattcta taatcaatga gatacacta tccgataacg 19560 aatgaagtct agcactagta tgccatttac ttaatatggt cgtcttggaa gttttat 19620 aagttaaaat atcatggtta tccaatttcc atctaatata ctttgtcgga ttatctatag 19680 tacacggaat aatgatggta tcattacatg ctgtatactc tatggtcttt gtagttgtta 19740 taacaaccaa cgtatagagg tatatcaacg atattctaac tcttgacatt ttttatttat 19800 ttaaaatgat acctttgtta tttattttat tctattttgc taacggtatt gaatggcata 19860 agtttgaaac gagtgaagaa ataatttcta cttacttatt agacgacgta ttatacacgg 19920 gtgttaatgg ggcggtatac acattttcaa ataataaact aaaaaaact ggtttaacta 19980 ataataatta tataacaaca tctataaaag tagaggatgc ggataaggat acattagtat 20040 gcggaaccaa taacggaaat cccaaatgtt ggaaaataga cggttcagac gacccaaaac 20100 atagaggtag aggatacgct ccttatagca aagtaacgat aatcagttac aacgaatgtg 20160 tactatctga cataaacata tcaaaagaag gaattaaacg atagagaaga tttgacggac 20220 catgtggtta tgatttattc acggcggata acgtaattcc aaaagatggt ttacgaggag 20280 cattcgtcga taagacggt acttatgaca aagtttacat tcttttcact gatactatcg 20340 gctcaaagag aattgttaaa attccgtata taacacaaat gtgcttaaac gacgaaggtg 20400 gtccatcatc gttgtctagt catagatggt cgacgttttct caagtcgaa ttagaatgtg 20460 acatcgacgg aagagttat agataaatta ttcattctaa acattaaaa acagataatg 20520 atacgatact atatgtattc ttcgatagtc cgcattatgt acctattcta tgaataccat 20580 taaacaatct tttctacgt caaattgga agatataca aagcaattgc cgtctccagc 20640 tcctgtata tgtctaccag ctggaaaagt tgttccacat accacgtttg aagtcataga 20700 aaaatataat gtactagatg atattataa gcctttatct aaccaaccta tcttcgaagg 20760 accgtctggc gttaaatggt tcgatataaa ggagaaggaa atgaacatc gggaatag 20820 atatacttc aaaagaaa attctatata ttcattcgat aaaaacta aaaaactcg 20880 cagtgcacaa gtcaatgcac aactattttc agtaatggta actgcgaaac cgttattat 20940 agcagatata gggataggag taggaatgcc acaatgaaa aaatactta aaatgtaatc 21000 ttaatcgagt acaccacacg acatgaaca aacataagac agattgct ggttatgctt 21060 gctgcgtaat atgcggtcta attgttggaa ttatttc agcgacacta ttaaaagttg 21120 window attagttcat acaccatca windows gataaagat gcatatatta 21180 gagaagattg tcctactgac tggataagct ataataataa atgtatccat tattactactg 21240 atcgaaaaac ctgggaggaa ggacgtaata catgcaagc tctaaatcca attcggatc 21300 taatttagat agagactcca acgagttaa gttttttag aagcattaga cgcggatatt 21360 gggtaggaga atccgaata ttaaaccaga taattttata gctaagaatg 21420 ccacgaagaa tggaaata tttgtagtac aacgaatact cccaaactgc attcgtgtta 21480 cactatataa cattacact acatttttat cataccacta cttcggttag atgttttaga 21540 aaaaataaa tatcgccgta ccgttcttgt ttttataaa atacatta acaattatca 21600 aattttttct ttaatatttt acgtggttga ccattctgg tggtaaaata atctcttagt 21660 gttggaatgg aatgctgttt aatgttccg cactcatcgt atatttgac gtatgcagtc 21720 acatcgttta cgcaatagtc agactgtagt tctatcatgc ttcctacatc agaaggagga 21780 acagttttaa agtctcttgg ttatcta ttgccattag tttcatga atccttttgtt 21840 ttatccactt cacattttaa ataaatgtcc actatacatt cttttgttaa ttttactaga 21900 tcgtcatggg tcatagaatt tataggttcc gtagtccatg gatccaaact agcaaacttc 21960 gcgtatacgg tatcgcgatt agtgtataca ccaactgtat gaaaattaag aaaacagttt 22020 aaaatcaa cagaaatatt taatcctccg tttgatacag atgcaccata tttggatt 22080 tcggattcac acgttgtttg tctgaggtgt tcgtcttag tgcttc ataacttcg 22140 attcccatat attctttatt gtcagaatcg cataccgatt tatcatcata cactgtttga 22200 aaactaaatg gtatacacat aaataatacg agtacattct gcaatattgt 22260 tatcgtaatt ggaaaaatag tgttcgagtg agttggatta tgtgagtatt ggattgtata 22320 ttttatttta tattttgta taagaataga atgctaatgt caagtttatt ccaatagatg 22380 tcttattaaa aacatatat aaaataac atggctgaa tggcataaa ttatcgagga 22440 tatctcaaaa aataataagt tcgaggatgc cgccatcgtt gattacaaga ctacaaagaa 22500 tgttctagct gctattccta acagaacatt tgccaagatt aatccgggtg aaattattcc 22560 tctcatcact aatcgtaata ttctaaaacc tcttattggt cagaaatatt gtattgtata 22620 tactaactct ctaatggacg agaacacgta tgctatggag ttgcttactg ggtacgcccc 22680 tgtatctccg atcgttatag cgagaactca taccgcactt atatttttga tgggtaagcc 22740 aacaacatcc agacgtgacg tgtatagaac gtgtagagat cacgctaccc gtgtacgtgc 22800 aactggtaat taaaataaaa agtaatattc atatgtagtg tcaattttaa atgatgatga 22860 tgaaatggat aatatccata ttgacgatgt caataatgcc ggtattggca tacagctcat 22920 cgattttag atttcattca gaggatgtgg aattatgtta tgggcatttg tattttgata 22980 ggatctataa tgtagtaaat ataaaatata atccgcatat tccatataga tataatttta 23040 ttaatcgcac gttaaccgta gatgaactag acgataatgt cttttttaca catggttatt 23100 ttttaaaaca caaattggt tcacttaatc ctagtttgat tgtctcatta tcaggaaact 23160 taaatataa tgatatacaa tgctcagtaa atgtatcgtg tctcattaaa aatttggcaa 23220 cgagtacatc tactatatta acatctaaac ataagactta ttctctacat cggtccaagt 23280 gtattatta aataggatat gattctatta tatggtataa agatataaat gacaagtata 23340 atggcatcta tgattttact gcaatatgta tgctaatagc gtctacattg atagtgacca 23400 tatacgtgtt taaaaaata aaaatgaact cttaattatg ctatgctatt agaaatggat 23460 aaaatcaaaa ttacggttga ttcaaaaatt ggtaatgttg ttaccatatc gtataacttg 23520 gaaaagataa ctattgatgt tacaccaaaa aagaaaagga tgtattatta gcgcaatcag 23580 ttgctgtcga agaggcaaaa gatgtcaagg tagaagaaa aaatattatc gatattgaag 23640 atgacgatga tatggatgta gaaagcgcat atacgatct ataaaaataa gtatataaat 23700 actttttatt tactgtactc ttactgtgta gtggtgatac cctactcgat tattttttta 23760 aaaaaaaat acttattctg attcttctaa ccatttccgt gttcgttcga atgccacatc 23820 gacgtcaaag ataggggagt agttgaaatc tagttctgca ttgttggtac gcacctcaaa 23880 tgtagtgttg gatatcttca acgtatagtt gttgagtagt gatggttttc taaatagaat 23940 tctcttcata tcattcttgc acgcgtacat ttttagcatc catcttggaa tcctagatcc 24000 ttgttctatt cccaatggtt tcatcaatag aagattaaac atatcgtacg aacacgatgg 24060 agagtaatcg tagcaaaagt aagcatttcc tttaatctta gatcccggat actggatata 24120 ttttgcagcc aacacgtgca tccatgcagc atttcctaca tatacccggc tatgtaccgc 24180 gttatcatcg actgtacgat acataatgtt accgtgttgc ttacattgct cgtaaaagac 24240 tttcgtcaat ttgtctcctt ctccgtaaat tccagtgggt cttaggcaac aagtatacaa 24300 ttttgctcca ttcatgatta cggaattatt ggctttcata accagttgct cggccatacg 24360 tttacttttt gcgtatacat gtcctggtga tatatcataa agggtatgct catggccgat 24420 gaatggatca ccgtgtttat tgggtcctat tgcttccatg ctactagtat agatcaaata 24480 cttgattcct aggtccacac aagctgccaa aatagtctgt gttccataat agtttacttt 24540 catgatttca ttatcggtgt attttccaaa tacatccact agagcagctg tatgaataat 24600 cagatttacc ccatttagcg cttctctcac cttatcaaag tcgtttatat cacattgtat 24660 atagtttata accttaactt tcgaggttat tggttgtgga tcttctacaa tatctatgac 24720 tctgatttct tgaacatcat ctgcactaat taacagtttt actatatacc tgcctagaaa 24780 tccggcacca ccagtaaccg cgtacacggc cattgctgcc acttataata tcaaactact 24840 tattctattt tactaaataa tggctgtttg tataataaac cacgataata tcagaggagt 24900 tatttacttt gaaccagttc atggaaaaga taaagtttta ggatcagtta ttggattaaa 24960 atccggaacg tatagtttga taattcatcg ttacggagat attagtcaag gatgtgattc 25020 cataggcagt ccagaaatat ttatcggtaa catctttgta aacagatatg gtgtagcata 25080 tgtttattta gatacagatg taaatatatt tacaattatt ggaaaggcgt tatctatttc 25140 aaaaaatgat cagagattag cgtgtggagt tattggtatt tcttacataa atgaaaagat 25200 atacatttt cttacaatta acgagaatgg cgtttgatat atcagttaat gcgtctaaaa 25260 caataaatgc attagtttac tttctactc agcaaata attagtcata cgtaatgaag 25320 ttaatgatac acactacact gtcgaatttg atagggacaa agtagttgac acgtttattt 25380 catataatag acataatgac tccatagaga taagaggggt gcttccagag gaactaata 25440 ttggttgcgc gttaatacg ccggttagta tgacttactt gtataag tatagtttta 25500 aactgatttt agcagaatat atagacaca gaatactat atccggcaat atttattcgg 25560 cattgatgac actagatgat ttggctatta aacagtatgg agacattgat ctattatta 25620 atgagaaact taaagtagac tccgattcgg gactatttga ctttgtcaac tttgtaagg 25680 atatgatatg ttgtgattct agaatagtag tagctctatc tagtcttag tctaaacatt 25740 gggaattgac aaaaaag taggtgta tggcattagc cgaacatata tctgatagta 25800 ttccaatatc tgagctatct agactacgat acaatctatg taagtatcta cgcgggcaca 25860 ctgagagcat agaggataaa tttgattatt ttgagacga tgattcgtct acatgttctg 25920 ccgtaaccga cagggaaacg gatgtataat tttagggata acagggtaat 25970 <210> 8 <211> 28837 <212> DNA <213> Vaccinia virus <400> 8 tagggataac agggtaatta cttattctga ttcttctaac catttccgtg ttcgttcgaa 60 tgccacatcg acgtcaaaga taggggagta gttgaaatct agttctgcat tgttggtacg 120 cacctcaaat gtagtgttgg atatcttcaa cgtatagttg ttgagtagtg atggttttct 180 aaatagaatt ctcttcatat cattcttgca cgcgtacatt tttagcatcc atcttggaat 240 cctagatcct tgttctattc ccaatggttt catcaataga agattaaaca tatcgtacga 300 acacgatgga gagtaatcgt agcaaaagta agcatttcct ttaatcttag atcccggata 360 ctggatatat tttgcagcca acacgtgcat ccatgcagca tttcctacat atacccggct 420 atgtaccgcg ttatcatcga ctgtacgata cataatgtta ccgtgttgct tacattgctc 480 gtaaaagact ttcgtcaatt tgtctccttc tccgtaaatt ccagtgggtc ttaggcaaca 540 agtatacaat tttgctccat tcatgattac ggaattattg gctttcataa ccagttgctc 600 ggccatacgt ttactttttg cgtatacatg tcctggtgat atatcataaa gggtatgctc 660 atggccgatg aatggatcac cgtgtttatt gggtcctatt gcttccatgc tactagtata 720 gatcaaatac ttgattccta ggtccacaca agctgccaaa atagtctgtg ttccataata 780 gtttactttc atgatttcat tatcggtgta ttttccaaat acatccacta gagcagctgt 840 atgaataatc agatttaccc catttagcgc ttctctcacc ttatcaaagt cgtttatatc 900 acattgtata tagtttataa ccttaacttt cgaggttat ggttgtggat cttctacaat 960 atctatgact ctgatttctt gaacatcatc tgcactaatt aacagtttta ctatatacct 1020 gcctagaaat ccggcaccac cagataaccgc gtacacggcc attgctgcca cttataatat 1080 caaactactt attctatttt actaataat ggctgtttgt samaaaacc acgataatat 1140 cagaggtt atttactttg aaccagttca tggaaaagat aaagttttag gatcagttat 1200 tggattaaaa tccggaacgt atagtttgat aattcatcgt tacggagata ttagtcaagg 1260 atgtgattcc ataggcagtc cagaaatatt tatcggtaac atctttgtaa acagatatgg 1320 tgtagcatat gttatttag atacagatgt aaatatattt acaattattg gaaaggcgtt 1380 atctatttca aaaaatgatc agagattagc gtgtggagtt attggtattt cttacataaa 1440 tgaaaagata atacatttc ttacaattaa cgagaatggc gtttgatata tcagttaatg 1500 cgtctaaaac aataaatgca ttagtttact tttctactca gcaaaataaa ttagtcatac 1560 gtaatgaagt taatgataca cactacactg tcgaatttga tagggacaaa gtagttgaca 1620 cgttattc atataataga cataatgact ccatagagat aagaggggtg cttccagagg 1680 aaactaatat tggttgcgcg gttaatacgc cggttagtat gacttacttg tataataagt 1740 atagttttaa actgatttta gcagaatata taagacacag aatactata tccggcaata 1800 tttattcggc attgatgaca ctagatgatt tggctattaa acagtatgga gacattgatc 1860 tattatttaa tgagaaactt aaagtagact ccgattcggg actatttgac tttgtcaact 1920 ttgtaaagga tatgatatgt tgtgattcta gaatagtagt agctctatct agtctagtat 1980 ctaaacattg ggaattgaca aataaaaagt ataggtgtat ggcattagcc gaacatatat 2040 ctgatagtat tccaatatct gagctatcta gactacgata caatctgt aagtatctac 2100 gcgggcacac tgagagcata gaggataaat ttgattttt tgagacgat gattcgtcta 2160 catgttctgc cgtaaccgac agggaaacgg atgtataattt tttttag cgtgaaggat 2220 atgataaaaa atatattgt tgtatttatc ccattccaat caccttatat gattctgtaa 2280 aaaaattata ctgtaacaca aaaggagt cttatagatg tatagaggtc agatactggt 2340 ttgataaact gtttattcca cataagtatg tttgacttta tggttagacc cgcatacttt 2400 aacaaatcac tgaaaatttgg agttaggtat tgacctca gatcagttg ccgttctgga 2460 acattaaatg tattttat gatatactcc aacgcattta tgtgggcata ciecaagtca 2520 ttactaatgg agtattccaa gagttttagt tgtctagtat ttaacaagg aagagatttc 2580 aacagactgt ttatgaactc gatgccgcc tcattgtcgc tatatattgat gatgtcgaat 2640 tctcccaata tcatcaccga tgagtagctc atcttgttat cgggatccaa gtttctaaa 2700 gatgtcatta aaccctcgat catgaatgga tttatcatca tcgtttat gttggacatg 2760 agcttagtcc gtttgtccac atctatagaa gatgatttct gattatttc atatatctct 2820 ctctttaact ccaggaactt gtcaggatgg tctactttaa tatgttctcg tctaagagat 2880 gaaaatcttt ggatggttgc acgcgacttt tctctaaagg atgacgttgc ccagatcct 2940 ctcttaaatg aatccatctt atccttggac aagatggaca gtctattttc cttagatggt 3000 ttaatatttt tgttacccat gatctaaaa ggtagaccta atcgtctcgg atgaccatat 3060 atttatttc agttttatta tacgcataaa ttgtaaaaaa tatgttaggt ttacgaaaat 3120 gtctcgtggg gcattaatcg ttttgaagg attggacaaa tctggaaaaacacaatg 3180 tatgaacatc atggaatcta taccggcaa cacgataaaa tatcttact ttcctcagag 3240 atccactgtc actggaaaga tgatagatga ctactaact cgtaaaaaaa cctataatga 3300 tcatatagtt aatctattat ttgtgcaaa tagatgggag tttgcatctt ttatacaaga acaactagaa caggaatta ctttaatagt tgatagatac gcattctctg gagtagcgta 3420 tgccgccgct aaagcgcgt caatgactct cagtaaggt tatgaatctg gattgcctaa 3480 acccgactta gttatattct tggaatctgg tagcaagaa attatagaa acgtcggcga 3540 ggaatttat gagatgtta cattccaca aaggtatta caggaata aaaaatgat 3600 tgagaagga gatattcatt ggcaaattat ttctctgaa ttcgaggaag atgtaagaa 3660 ggagttgatt aagaatag ttatagaggc tatacacacg gttactggac cagtggggca 3720 actgtggatg taatagtgaa attacatttt ttaataag atgttagtac agtgttataa 3780 atggatgaag catattactc tggcaacttg gatcagtac tcggatacgt gtccgatg 3840 cataccgaac tcgcatcaat atctcaat gttattgcca agatagaac tatagataat 3900 gatatattaa aaggacat tgtaaatttt atcatgtgta gatcaaactt ggataatcca 3960 tttatctct tcctagatac tgtatatact attatagatc aagagatcta tcagaccgaa 4020 ttgattaattt cattagacga caatgaatt atcgattgta tgttaataa gtttatgagc 4080 ttttatagg atacctaga aaatagta gatgctatca ttactctaaa atatataatg 4140 aaatccag attttaaaac tacgtagca gaagtactcg gttccagaat agcggatata 4200 ataataac aagtgatacg tgagaata ctacaattgt ctaatatat ccgcgaacga 4260 tattgtgaa atattaaaaaaaatactt ttttatta atgacgtctt ttcgtgaatt 4320 tagaaaatta tgctgtgcta tatatcaccc atcaggatat aaagaaaaat ctaaattaat 4380 tagagacttt atacagata gggatgataa atatttgatc atttaagctat tgcttcccgg 4440 Random Random RandomTaxaatt Aaaatt Random 4500 attackaaaa tctcaggag attgctaca agatttagga tagggatata taggagac 4560 tattagtaca ttctcaag aaaacacaga aatccgtcca agagataaaa gcattttaac 4620 tttagagac gtggatagtt tcttactac gttatcatcc gtaactaag aattgcatca 4680 aaaaatta ttgactgag tagcatctgt ttgtacatgt atgatttaa atgtgtagt 4740 catgcttatt gataaagatc taaaaattta agcgggccct cggtacgtac ttaacgctat 4800 tagtcctcat gcctatgatg tttttagaaa atctaataac tgaaagaga taatagaaaa 4860 tgcatctaaa caaatctag actctatatc tatttctgtt atgactccaa ttaatcccat 4920 gttagcggaa tcgtgtgatt ctgtcaataa ggcgtttaaa aaatttccat caggaatgtt 4980 tgcggaagtt aaatacgatg gtgaagagt acagttcat aaaataata acgagtttgc 5040 cttctttagt agaaacatga aaccagtact ctctcataa gtggattac tcaagaat 5100 cataccgaaa gcatttaaaa aagctacgtc tatcgtattg gattctgaaa ttgttcttgt 5160 agacgaacat atgtaccgc tcccgttttgg aagtttaggt atacacaaa agaagaata 5220 taaaaactct aacatgtgtt tgttcgtgtt tgactgttg tactttgatg gattcgatat 5280 gacggacatt ccattgtacg aacgaagatc ttttctcaa gatgttatgg ttgaatacc 5340 chaatagaata gtattctcag agttgacgaa tattagtaac gagtctcagt taactgacgt 5400 attggatgat gcactaacga gaaaattaga aggattggtc ttaaaagata ttaatggagt 5460 atacgaaccg ggaagagaa gatggttaa aaaagcga gactttga acgaggggttc 5520 catggcagat tctgccgatt tagtagtact aggtgcttac tatggtaag gagcaaaggg 5580 tggtatcatg gcagtctttc taatgggttg ttacgacgat aaatccggta aatggaagc 5640 ggttaccaag tgttcaggac acgatgata tacgttaagg gagttgcaag accattaaa 5700 gatgattaa atttaacagg atcccaaaa attccagag tggttggtag ttataaaat 5760 ctatattccc gattttgtag taggattcc aaaacaatct cagatatggg aaatttcagg 5820 agcagagtttt acatcttcca agtcacatac agcgaatgga atatcgatta gatttcctag 5880 attactagg atagagagg ataaacgtg gaagaatct actcatctaa acgatttagt 5940 aaacttgact aaatcttaat agttacatac aactgaaaa ttaaatac accattagt 6000 tggtggtcgc catggatggt gttattgtat actgtctaaa cgcgttagta aaacatggcg 6060 aggaaaaa tcatataaaa atgatttca tgattaacc atgttgtgaa aaagttaaga 6120 acgttcacat cgacggacaa tctaaaaaaca atacagtgat tgcagatttg ccatatctgg 6180 ataatgctgt atcggatgta tgcaatcga tatatatata gtatcaagaa tatccagatt 6240 tgctaatttg aaagatag atgacgatga caagactcct actggtgtat ataattattt 6300 taaacctaaa gatgccattc ctgttattat atccatagga aaggatagag atgttttgtga 6360 actattaatc tcatctgata aagcgtgtgc gtgtatagag ttaaattcat ataaagtagc cattcttccc atggatgttt ccttttttac caaaggaat gcatcattga ttattctcct gtttgatttc tctatcgatg cggcacctct cttaagaagt gtaaccgata ataatgttat fathers caccagcgtc tacatgacga gcttccgagt tccaattggt tcaagtttta cataagtata aagtccgact attgttctat attatatatg gttgttgatg gatctgtgat gcatgcgata gctgataata gaacttacgc aaatattagc aaaaatat tagacaatac 6780. gatgagtgta gatgctgtta ttttgaacca cagattagga ttcttgatag agatgagatg ctcaatggat catcgtgtga tatgaacaga cattgtatta tgatgaattt acctgatgta ggcaaatttg gatctagtat gttggggaa tatgaacctg acatgata gattgctctt ttggtggctg gtaatttaat aagaaatcga gactacattc ccggggagacg aggatatagc tactacgttt acggtatagc ctctagataa tttttttaag cacgaaataa 7080. aaaacataat tttaaaccaa tctatttcat actatttgt gtgatcacca tggacataaa gatagatatt agtatttg gtgataaatt tacggtgact actaggagggg aaaatgaaga 7140 aaaaaaaaaa tattactc tccaaaaga aaaaactact gatgttatca aacctgatta 7200 tcttgagtac gatgacttgt tagagaga tgagatgttt actattctag aggaatattt 7260 tatgtacaga ggtctattag gcctcagaat aaatatgga cgactcttta acgaattaa 7320 aaaattcgac atgatgcgg aagaacatt cggtactata gagaactca agcagaact 7380 Taggattaat tctgagagg gagcagata ctttataat tataaagg tacaaaca 7440 ggatatcgtc aaacttactg tatacgattg catatctatg ataggattgt gtgcatgcgt 7500 ggtagatgtt tggagaatg agaaactgtt ttctagatgg aaatattgtt tacgagcgat 7560 taaactgttt attaatgatc acatgcttga windowaaa tctatactgc agatagact 7620 agtgtatgtg gaatgtcat agaaagttaa aagttaatga gagcaaaat atataggtt 7680 gtattccata ttgttattt tttctgtaat agttaaaaaa tacattcgat ggtctatcta 7740 tcagattatt atgtgttata aggtacttt tctcataata actagagta tgagtagat 7800 agtgttttc aaaacatata aatctaaaat tgatggatga gatatacagc tattaatttc 7860 gaaaatatat tttaatctga taactttaaa catggatttt tgatggtggt ttaagtttta 7920 aaaaaaagat ttgttattg tagtatatga taatatcaa aagatgata taagaattt 7980 gctgactgta tgtactattt tttacattac tacattggct acggcagata tacctactc 8040 gtcactgcca cacgctccgg taaacggggc atgtgacgag ggagaatatc ttgataagag 8100 gcataatcaa tgttgtaatc agtgtccacc tggagaattt gccaaggtca gatgtagtgg 8160 tagtgataac aaaatgtg aacgctgccc acccataca tataccgcaa tccccaatta 8220 ctctaatgga tgtcatcaat gtagaaaatg cccaacagga tcatttgata aggtaagtg 8280 taccggaaca cagaacagta aatgttcgtg tcttcctggt tggtattgcg ctactgattc 8340 ttcacagact gaagattgtt gaaattgtgt accaaaagg agatgtccat gcggatactt 8400 tggtggaata gatgaacaag gaatcctat ttgtaaatcg tgctgtattg gtgaatattg 8460 caactaccta cgtaattata gacttgatcc attttctcca tgcaactat ctaaatgtaa 8520 ttaattatga tttgatgat aatgttacca tacattatat cgctacttgg ttagtgtatt 8580 attcagtag agacctatt aaattact tatctttga cgatcttgtt ataattataa 8640 tataaaaact tatggcatag taacttata ttgctacac gataaattcg taataatctg 8700 ttttgttca atttttaata ggaatctaca ggcataaaa taaaaata atttatata 8760 tactcttaca acgccatcat gatagcagc agtaaattta ttgctgttat taatggattt 8820 agaaatagtg gacgattttg tgattaat atagttatta atgatgaaag gataaacgct 8880 cataaactca tcctatctgg agcctccgaa tattttcca ttctgttttc caataatttt 8940 atcgattcta atgaatcga agttaatcta agtcattctag attcaag cgtaaacgat 9000 ttgatcgatt atatttatgg gataccttg agcctaacta acgataacgt gaaatatatt 9060 ctttcaaccg ctgatttt acaaattgga tctgctatta cggagtgtga aattacata 9120 cttaaaaatc ttgttctaa aactgtatc gatttctaca tatacgctga taatataat 9180 aacaagaaaa tagaatcagc gtcgtttaac acatattac aaatatttt gagactcatc 9240 aacgatgaaa actttaaata cttaacagag gaatcaatga taaaaatttt aagcgatgat 9300 atgttaaata taaaaaatga ggatttcgcc ccactaattc tcattaaatg gttagagagt 9360 actcaacaat catgcaccgt cgagttactt aaatgcctca gaatatcatt gctttcccca 9420 caagttataa aatcacttta tagtcatcaa ctggttagtt caatctacga atgtataaca 9480 ttcttaaaca atatagcatt cttggatgaa tcatttccta gataccatag catcgagttg 9540 atatctatcg gtataagtaa ttcgcgtgat aaaatttcca taaactgcta caatcataaa 9600 aaaatacat gggaaatgat atcttcacgt agatataggt gtagttcgc agtggccgtc 9660 ctggataata ttatctatat gatgggtgga tatgatcagt ccccgtatag aagttcaaag 9720 gttatagcgt acaatacatg tacaaattct tggatatatg atataccaga gctaaaatat 9780 cctcgttcta attgtggggg actggctgat gacgaataca tttattgtat aggcggcata 9840 cgcgatcagg attcatcgtt gacatctagt attgatagat ggaagccatc aaaaccatat 9900 tggcagaagt atgctaaaat gcgcgaacca aaatgtgata tgggggttgc gatgttaaac 9960 ggattaatat atgtcatggg tggaatcgtt aaggtgaca cgtgtaccga cgcactagag 10020 agtttatcag aagatggatg gatgaagcat caacgtcttc caataaaaat gtccaatatg 10080 tcgacgattg ttcatgatgg caatttat atatctggag gttacaacaa tagtagtgta 10140 gttaatgtaa tatcgaatct agtccttagc tataattcga tatatgatga atggaccaaa 10200 ttatcatcat taaatattcc tagattaat cccgctctat ggtcagcgca taataatta 10260 tatgtaggag gaggaatc tgatgtt storm tatctgaac atachgataa 10320 gaaaaagatt gttggacatt ggataatggt cacgtgttac cacgcaatta tataatgtat 10380 aaatgcgaac cgattaaca taaatatcca ttggaaaaa cacagtacac gatgatttt 10440 ctaaagtatt tggaagtttt tataggtagt tgatagaaca aatacataa tttgtaaaa 10500 aaatcact tttatacta ataatcacg attaccata cttttgttac taatatcatt 10560 agtatacgct acaccttttc ctcagacatc taaaaaaata ggtgatgat g caacctatc 10620 atgtaatcga aaatacaa atgactacgt tgttatgagt gcttggtata aggagcccaa 10680 ttctattatt cttttagctg ctaaaagcga cgtcttgtat tttgaaatt ataccaagga 10740 taaaatatct tacgactctc catacgatga tctagttaca actatcacaa ttaaatcatt 10800 gactgctaga gatgccggta cttatgtatg tgcattcttt atgacatcaa ctacaaatga 10860 cactgataaa gtagattatg aagaatactc cacagagttg attgtaaata cagatagtga 10920 atcgactata gacatatac tatctggatc tacacattca ccggaaacta gttctgagaa 10980 acctgattat atagataatt ctaattgctc gtcggtattc gaaatcgcga ctccggaacc 11040 aattactgat aatgtagaag atcatacaga caccgtcaca tacactagtg atagcattaa 11100 tacagtaagt gcaacatctg gagaatccac aacagacgag actccggaac caattactga 11160 taaagaagat catacagtta cagacactgt ctcatacact acagtaagta catcatctgg 11220 aattgtcact actaaatcaa ccaccgatga tacgtacaat gataatgata cagtaccacc 11280 aactactgta ggcggtagta caacctctat tagcaattat aaaaccaagg actttgtaga 11340 aatatttggt attaccgcat taattatatt gtcggccgtg gcaatattct gtattacgta 11400 ttatatatgt aataaacgtt cacgtaaata caaaacagag aacaaagtct agatttttga 11460 cttacataaa tgtctgggat agtaaaatct atcatattga gcggaccatc tggttcagga 11520 aagacagcca tagccaaaag actatgggaa tatatttgga tttgtggtgt cccataccac 11580 tagatttcct cgtcctatgg aacgagaagg tgtcgattac cattacgtta acagagaggc 11640 catctggaag ggaatagccg ccggaaactt tctagaacat actgagtttt taggaaatat 11700 ttacggaact tctaaaacag ctgtgaatac agcggctatt aataatcgta tttgtgtgat 11760 ggatctaaac atcgatggcg ttagaagtct taaaaatacg tacctaatgc cttactcggt 11820 gtatataaga cctacctctc ttaaaatggt tgaaaccaag cttcgttgta gaaacactga 11880 agctaacgat gagattcatc gtcgcgtgat attggcaaaa acggatatgg atgaggccaa 11940 cgaagcaggt ctattcgaca ctattattat tgaagatgat gtgaatttag catatagtaa 12000 gttaattcag atactacagg accgtattag aatgtatttt aacactaatt aaagacttaa 12060 gacttaaaac ttgataatta atatataac tcgtttttat atgtgtctat ttcaacgtct 12120 aatgtattag ttaatatta aaacttacca cgtaaaactt aaaatttaaa atggtattc 12180 attgacagat agatcacaca tatgaactt tcaggactt gtgttaactg acaattgcaa 12240 aaatcaatgg gtcgttggac cattaatagg aaaggtgga tttgtagta ttatactac 12300 taatgacaat attatgtag taaaaataga gcccaagct aacggatcat tattaccga 12360 acaggcattt tatactagag tacttaacc atccgttac gagaatgga aaaaatctca 12420 caataaag cacgtaggtc ttcacgtg caagcattt ggtctataca aatccattaa 12480 tgtggaatat cgattcttgg taattatag attaggtgca gatctagatg cggtgatcag 12540 agccaataat atagattac caaaaggtc ggtgatgttg atcggaatcg aaatcttaa 12600 taccatacaa tttgcacg agcaagta tctcacgga gattaag cgagtaat 12660 agtcttagat aaatagata agaataattt atatctagtg gattacggat tggttttctaa 12720 attcatgtct aatggcgaac atgttccatt tatagaaat ccaataaa tggataacgg 12780 tactctagaa tttacaccta tagattcgca taaggatac gttgtatcta gacgtggaga 12840 tctagaaaca cttggatatt gtatgattag atggttggga ggtatcttgc catggactaa 12900 gatatctgaa aaagaatt gtgcattagt aagtgccaca aaaacagaat atgttaaca 12960 tactgcgact ttgttaatga ccagtttgca atatgcacct agagaattgc tgcaatatat 13020 taccatgta aactctttga catatttga ggaacccaat tacgacgagt ttcggcacat 13080 attaatgcag gtgtatatt attaagtgtg gtgttttggtc gatgtaaaat tttgtcgat 13140 aaaaattaaaaataactta atttatt gatctcgtgt gtacaaccga atcatggcg 13200 atgttttacg cacacgctct cggtgggtac gacgagaatc ttcatgcctt tcctggaata 13260 tcatcgactg tgccaatga tgtcaggaaa tattctgttg tgtcagttta taataacaag 13320 tatgacattg taaagacaa atatatgtgg tgttacagtc aggtgaacaa gagatatatt 13380 ggagcactgc tgcctatgtt tgagtgcaat gatatctac aaattggaga tccgatccat 13440 gatcaagaag gaaatcaaat ctctatcatc acatatcgcc acaaaaacta ctatgctcta 13500 agcggaatcg ggtacgagag tctagacttg tgtttggaag gagtagggat tcatcatcac 13560 gtacttgaag caggaaacgc tgtatatgga aaagttcaac atgattattc tactatcaaa 13620 gagaaggcca aagaaatgag tacacttagt ccaggaccta taattgatta ccacgtctgg 13680 ataggagatt gtatctgtca agttactgct gtggacgtac atggaaagga aattatgaaa 13740 atgagattca aaaagggtgc ggtgcttccg atcccaaatc tggtaaaagt taaacttggg 13800 gagaatgata cagaaaatct ttcttctact atatcggcgg caccatcgag gtaaccacct 13860 ctctggaaga cagcgtgaat aatgtactca tgaaacgttt ggaaactata cgccatatgt 13920 ggtctgttgt atatgatcat tttaatattg tgaatggtaa agaatgctgt tatgtgcata 13980 cgcatttgtc taatcaaaat cttataccga gtactgtaaa aacaaatttg tacatgaaga 14040 ctatgggatc atgcattcaa atggattcca tggaagctct agagtatctt agcgaactga 14100 aggaatcagg tggatggagt cccagaccag aaatgcagg atttgaatt ccagatggag 14160 tggagacac tgaatcaatt gagagattgg cagaggatt cttcataga tcagaacttc 14220 aggctggtaa atcagttaaa ttgtaatt ctattaattg ttaaacatat acatctgttt 14280 cagctaagca actaagaaca cgtatacggc agcagcttcc ttctatactc tcatcttta 14340 ccaagggtgg atatttgttc attggattg ataatatac acacaagta attggattca 14400 cggtgggtta cgactaccctc agactggtag agaatgaat agaaagcat atcaaagac 14460 tttgtgttgt gtatttctgt gagagaaag aggacatcaa gtacacgtgt cgattcatca 14520 aggtatataa acctggggat gagactacct cgacatacgt gtgcgctatc aaagtggaaa 14580 gatgctgttg tgctgtgttt gcagattggc cagaatcatg gtatatggat actaatggta 14640 tcaataagta ttctccagat gatgggtgt cacatataaa attttatta atgtaataga 14700 gaaaata taggttgta attackcatact aaaattaat tagtactgt 14760 tatctctttt ttaactacc aactaactat atactatta atacatcgta attatagttc 14820 ttaacatcta ttaatcatta attcgcttct ttaattttt ataactaac attgttaatt 14880 gaaaagggat aacatgttac agaatataaa ttatatatgg attttttaa aaaggaaata 14940 cttgactgga gtatatattt atctcttcat tatatatagcac gcgtgttttc caatttttcc 15000 acatcccata taatacagga ttataatctc gttcgaacat acgagaaagt ggataaaaca 15060 atagttgatt tttatctag gttgccaaat ttattccata tttagaata tggggaaat 15120 attctacata tttattctat ggatgatgct aatacgaata ttataatttt ttttctagat 15180 agagtattaa atattaataa gaacgggtca tttatacaca atctcgggtt atcatcatcc 15240 attaatataa aagaatatgt atatcaatta gttaataatg atcatccaga taataaggata 15300 agactaatgc ttgaaaatgg acgtagaaca agacattttt tgtcctatat atcagataca 15360 gttaatatct atatatgtat tttaataaat catggatttt atatagatgc cgaagacagt 15420 tacggttgta cattattaca tagatgtata tatcactata agaaatcaga atcagaatca 15480 tacaatgaat taattagat attgttaat atgtccg atgtagata aaagatacg 15540 tacggaaca caccttttat cctattatgt aaacacgata tcaacgt ggaattgttt 15600 gagatatgtt tagagaatgc taatatagac tctgtagact ttaatagata tacaccctt 15660 cattatgtct catgtcgtaa taaatatgat ttgtaagt tattaatttc taaggagca 15720 aatgttaatg cgcgtaataa attcggaact actccatttt attgtggaat tatacacggt 15780 atctcgctta windows ttggaatca gacagagt windows windows 15840 atagttcgtc atttaataat ttttgatgct gttgaatctt tagattatct attatccaga 15900 ggagttattg atattaacta tcgtactata tacaacgaaa catctattta cgacgctgtc 15960 agttataatg cgtataatac gttggtctat ctattaaaca gaaatggtga tttgagacg 16020 attack gtggatgtac atgtttcg gaggagtcg storm c'aata 16080 atggaagtac tattgtctaa acgaccatct tgaaaatta tgatacagtc tatgatagca 16140 attackaac ataacagca taatgcagat ttattgaaa tgtgtataaa atactgcg 16200 tgtatgaccg attack tcttatagat gtacagtcgc tacagcaata taaatggtat attttaagat gtttcgatga aatagatatc atgaagagat gttatataaa aaataaaact gtattccaat tagttttttg tatcaaagac attaactt cggtaaacat 16380. gtattccaat tagttttttg ccttctttcg tgaatgcac tagtctcgac gtatacgga gtcgtgtacg taatatcata gcatctatta gatatcgtca gagattatt agtctattat ccaagaagct ggatgcggga gataaatggt cgtgttttcc tacgaaata aatataaaa tattggaaaa ctttaacgat aacgaactgt ccacatatct aaaaatctta 16620. aacgaactgt ccacatatct ggataaaatc acactacatc attgtttcct tttagtgctc gacagtgtat actattttta acactcataa ataaaaatga aaacgatttc cgttgttacg ttgttatgcg tactacctgc tgttgtttat tcaacatgta ctgtacccac tatgaataac gctaattaa cgtctaccga aacatcgttt fatherac agaaagttac atttacatgt gatcagggat atcattcttt ggatccaaat gctgtctgcg aaacagataa atggaaatac gaaaatccat gcaagaaaat 16920 gtgcacagtt tctgattatg tctctgaact atataataaa ccgctatacg aagtgaattc 16980 caccatgaca ctaagttgca acggcgaaac aaaatatttt cgttgcgaag aaaaaaatgg 17040 aaatacttct tggaatgata ctgttacgtg tcctaatgcg gaatgtcaac ctcttcaatt 17100 agaacacgga tcgtgtcaac cagttaaaga aaaatactca tttggggaat atataactat 17160 caactgtgat gttggatatg aggttattgg tgcttcgtac ataagttgta cagctaattc 17220 ttggaatgtt attccatcat gtcaacaaaa atgtgatata ccgtctctat ctaatggatt 17280 aatttccgga tctacatttt ctatcggtgg cgttatacat cttagttgta aaagtggttt 17340 tatactaacg ggatctccat catccacatg tatcgacggt aaatggaatc ccatactccc 17400 aacatgtgta cgatctaacg aaaaatttga tccagtggat gatggtcccg acgatgagac 17460 agatttgagc aaactctcga aagacgttgt acaatatgaa caagaaatag aatcgttaga 17520 agcaacttat catataatca tagtggcgtt aacaattatg ggcgtcatat ttttaatctc 17580 cgttatagta ttagtttgtt cctgtgacaa aaataatgac caatataagt tccataaatt 17640 gctaccgtaa atataaatcc gttaaaataa ttaataatta ataacgaaca agtatcaaaa 17700 gattaaagaa ttatagctag aatcaattga gatgtcttct tcagtggatg ttgatatcta 17760 cgatgccgtt agagcatttt tactcaggca ctattataac aagagattta ttgtgtatgg 17820 aagaagtaac gccatattac ataatatata caggctattt acaagatgcg ccgttatacc 17880 gttcgatgat atagtacgta ctatgccaaa tgaatcacgt gttaaacaat gggtgatgga 17940 tacacttaat ggtataatga tgaatgaacg cgatattact gtatgtgtgg gcaccggaat 18000 actattcatg gaaatgtttt tcgattacaa taaaaatagt atcaacaatc aactaatgta 18060 tgatataatt aatagcgtat ctataattct agctaatgag agatatagaa gcgcttttaa 18120 cgacgatggt atatacatcc gtagaaatat gattaacaag ttgtacggat acgcatctct 18180 aactactatt ggcacgatcg ctggaggtgt ttgttattat ctgttgatgc atctagttag 18240 tttgtataaa taattatttc atatactag ttaaattttt agattttaa atgtataaaa 18300 aactaataac gtttttattt gtaataggtg cagtagcatc ctattcgaat aatgagtaca 18360 ctccgtttaa taaactgagt gtaaaactct atatagatgg agtagaat atagaaaatt 18420 catatactga tgatataat gattggtgt taaattta agagtacaca atttctatta 18480 ttacagagtc atgcgacgtc ggatttgatt ccatagatat agatgttata aacgactata 18540 aaattattga tatgtatacc attgactcgt ctactattca acgcagaggt cacacgtgta 18600 gatatctac caattatca tgccattatg ataagtaccc ttatattcac aaatatgatg 18660 gtgatgagcg acatattct attactgcag agggaaatg ctataaagga aataaatatg 18720 aaatagtat gatcacgat gatacct tgagaaaca tactcttaa attggatcta 18780 cttatatatt tgatcgtcat ggacatagta atacatatta ttcaaatat gatttttaaa 18840 aatttaaaat atattacac ttcagtgaca gtagtcaat aaaacaac accatgagat 18900 attatataat tctcgcagtt ttgttcatta atagtataca tgctaaaata actagttata 18960 agtttgaatc cgtcaatttt gattccaaaa ttgaatggac tggggatggt ctatacaata 19020 tatcccttaa aaattatggc atcaagacgt ggcaaacaat gtatacaaat gtaccagaag 19080 gaagatacga catatccgca tttccaaaga atgatttcgt atctttctgg gttaaatttg 19140 aacaaggcga ttataaagtg gaagagtatt gtacggact atgcgtcgaa gtaaaattg 19200 gaccaccgac tgtaacatta actgaatacg acgaccatat caatttgtac atcgagcatc 19260 cgtatgctac tagaggtagc aaaaagattc ctatttacaa acgcggtgac atgtgtgata 19320 tctacttgtt gtatacggct aacttcacat tcggagattc taagaacca gtaccatatg 19380 atatcgatga ctacgattgc acgtctacag gttgcagcat agactttgtc aacaagaaa 19440 aagtgtgcgt gacagcacag ggagccacag aagggtttct cgaaaaaatt actccatgga 19500 gttcggaagt atgtctgaca cctaaaaaga gtgtatatac atgcgcaatt agatccaaag 19560 aagatgttcc caatttcaag gacaaaatgg ccagagttat caagaaaa tttaataaac 19620 agtctcaatc ttatttaact aaatttctcg gtagcacatc aaatgatgtt accactttc 19680 ttagcatgct taacttgact aaatattcat actatttt tattaatgat acaaaacga 19740 aaaaactg catattatac actggttaac gcccttatag gctctacca tttcagat 19800 gaggtccctg attatagtcc ttctgttccc cttatcatc tactccatgt ttattagacg 19860 atgtgagaag actgaagagg aaacatgggg attgaaata gggttgtgta taattgccaa 19920 agattttat cccgaaagaa ctgattgcag tgttcatctc ccaactgcaa gtgaaggatt 19980 gataactgaa ggcaatggat tcaggtat acgaaacacc gataattat aaaaaaagca 20040 atgtgtccgc tgtttccgtt aaatacta tttcgtaac tggcggatta ttcataaata 20100 actctaatag cacgatcgtg gttaacaata tggaaaact tgacatttat aaagacaaac 20160 aatggtcgat tatagaaatg cctatggcta gggtatatca cggcatcgac tcgacatttg 20220 gatgttata tttgccgga ggtctatccg ttaccgaaca atatggtaat ttagagaaaa 20280 acacgagat atcttgttac aatcctagaa cgaataagtg gtttgatatt tcatatacta 20340 tttataagat atccatatca tcattgtgta aactaaataa cgtctctat gtatttagta 20400 aggacattgg atatgtggaa aagtatgatg gtgcatggaa gttagtacat gatcgtctcc 20460 ccgcttaaa ggcattatca acttctcctt attgattgaa atgaaaata taatagttt 20520 ttatgtatag cagtattacc ctatagtttt attgcttact actaacatgg atacagatgt 20580 tachaatgta gagatatca t'atgaat agatagagag aaagaaa tactaaaaaa 20640 tgtagaattt gaaataata aaaacattaa ccaatgaat attagagaga 20700 agcactcgtt attack gtagtaatag tgattccatt gataaagaag ttatagaatg 20760 tatctgtcac gatgtaggaa tatagatcat atctaat tttaatc gatcaaac 20820 aaaaaaca actcgttatt acatagcagg catggaatcc ttcaagtatt gttttgataa 20880 cgatggcaag aaatggatta tcggaatac tttatattct ggtaattcaa tactatatata 20940 gttcagaaaa atttcacta gttcgttcta cattacgta atgagatag atcacaaatc 21000 acacaagcca ttgttgtctg aaatacgatt ctatatatct gtattggatc ctttgactat 21060 cgacaactgg acacgggaac gtggtataaa gtatttggct attccagatc tgtatggaat 21120 tggagaaacc gatgattata tgttcttcgt tataaaaaat tcgggaag tattcgcccc 21180 aaaggatact gatcagtct tcgaagcatg tgtcactatg ataacacgt tagtttat 21240 acactctcga ggatttaccc atggaaaat agaaccgagg atatactga ttagaataa 21300 acgtctttca ctattgact attcgaac taacaacta tacagagtg gaactcaca 21360 tatagattac aacgaggaca tgataacttc aggaattac attatatgt gtgtagaca 21420 tcatcttgga gcaacagtttt caaacgagg agatttagaa atgttgggat attgcatgat 21480 agaatggttc ggtggcaaac ttccatgga aaacgaaagt agtataaaag taaaaaca 21540 aaaaaaagaa tataaaaaat ttatagctac tttcttgag gactgttttc ctgaaggaaa 21600 tgaacctctg gattagtta gatatataga attagtatac acgttagatt attctcaaac 21660 tcctaattat aghagactac gtaaactgtt tatacagat tgaaattata ttcttttttt 21720 atagagtgtg gtagtgttac ggatatctaa tattaatatt agactatctc tatcgcgcta 21780 cacgaccaat atcgattact atggatatct tcagggaaat cgcatcttct atgaaaggag 21840 agaatgtatt tatttctcca gcgtcaatct cgtcagtatt gacaatactg tattatggag 21900 ctaatggatc cactgctgaa cagctatcaa aatatgtaga aacggaggag aacacggata 21960 aggttagcgc tcagaatatc tcattcaaat ccatgaataa agtatatggg cgatattctg 22020 ccgtgtttaa agattcctttt ttgagaaaaa ttggcgataa gtttcaaact gttgacttca 22080 ctgattgtcg cactatagat gcaatcaaca agtgtgtaga tatctttact gaggggaaaa 22140 tcaatccact attggatgaa ccattgtctc ctagcaatta gtgccgtata cttaaagca 22200 aaatggttga cgccattcga aaaggaattt accagtgatt atccctttta cgtatctccg 22260 acggaaatgg tagatgtaag tatgatgtct atgtacggca aggcatttaa tcacgcatct 22320 gtaaaggaat cattcggcaa cttttcaatc atagaactgc catatgttgg agatactagt 22380 atgatggtca ttcttccaga caagattgat ggattagaat ccatagaaca aaatctaaca gatacaaatt ttagaaatg gtgtgacttt atggatgcta tgtttataga tgttcacatt cccaagttta aggtaacagg ctcgtataat ctggtggata ctctagtaaa gtcaggactg acagaggtgt tcggttcaac tggagattat agcaatatgt gtaattcaga tgtgagtgtc gacgctatga tccacaaaac gtatagat gtcaatgaag agtacaga agcagctgca gcaacttgtg cactggtgtc agactgtgca tcaacaatta caaatgagtt ctgtgtagat catccgttca tctatgtgat taggcatgtt gatggcaaaa ttcttttcgt tggtagat 22800. tgctctccaa caactaattg ttaaccattt tttttaaaaa aatagaaaaa acatgtggta ttagtgcagg tcgttattct tccaattgca attggtaaga tgacggccaa ctttagtacc cacgtctttt caccacagca ctgtggatgt cacagactga cactattga tgacgtcaaa 23040. caatgtttga ctgaatat ttattggtcg tcctatgcat accgcaacag gcaatgcgct ggacagttgt attccacact cctctctttt cggaattgt gttcatcgac attcgcgagc tggtaaaaca tatgccgtgg gatgatgtca aagattgtgc agaaatcatc cgttgttata taccggatga gcaaaaaacc atcagagaga tttcggccat catcggactt tgtgcatatg ctgctactta ctggggaggt gaagaccatc ccactagtaa cagtctgaac gcattgtttg tgatgcttga gatgctaaat tacgtggatt fathercatcat attccggcgt atgaattgat gagttgtaca tcttgacatt ttcttctttc ttctcttctc cctttcccag 23460. aaacaaactt tttttaccca ctataaaata aaatgagtat actacctatt atatttcttt ctatattttt ttattcttca ttcgttcaga cttttaacgc gtctgaatgt atcgacaaag ggcaattt tgcatcattc atggagttag aaaacgagcc agtaatctta ccatgtcctc aaataatac gctatcatcc ggatataata tattagatat tttatgggaa aaacgaggag cggataatga tagatatta ccgatagata atggtagcaa tatgctaatt ctgaacccga cacaatcaga ctctggtatt fathers ttaccacga cgaacctac tgtgacatga tgtcgttaaa tttgacaatc gtgtctgtct cagaatcaa tatagatctt atctcgtatc 23820 cacaatagt aaatgagaga tctactggcg aaatggtag tcccaatatt atgcattta 23880 ttgctagtaa cgtaaacgca gatatat ggagcggaca tcgacgcctt agaaataaga 23940 gacttaaca acggacacct ggaattatta ccatagaaga tgttagaaaa atgatgctg 24000 gttattatac atgtgtttta gatatatat acggtggcaaacatataac gtaaccagaa 24060 ttgtaaaatt agaggtacgg gataaaata taccttctac tatgcaatta ccagatggca 24120 ttgtaacttc ataggtagt aatttgacta ttgcgtgtag agtatcgttg aggcctccca 24180 caacggacac cgacgtcttt tggataagta atggtagta ttacgaagaa gatgatgggg 24240 acggaaacgg tagatagt gtagcaata aaatctatat gaccgataag agacgtgtta 24300 ttacatcccg gttaaacatt aatcctgtta aggaagaga tgctacaacg tttacgtgta 24360 tggcgtttac tattcctagc atcagtaaaa cagttactgt tagtataacg tgaatgtatg 24420 ttgttacatt tccatgtca ttgagtttat aagaattttt attackatc ttccacaag 24480 caattgacga acgtattgct atgattact cccacgatac tatgcatatt attaatcatt 24540 aacttgcaga ctatacctag agctattttg acatactcgt gttcttgtgt aattgcagta 24600 tctatattat taagtacgt aaatcttagct atagttttat tattaattt tagatatat 24660 accgtctcct tattttaa attgccaca tcctttatta atcatgaat gggaatttct 24720 atgtcatcgt tagtatattg tgaacaaca gagcagatat ctataggaaa gggtggaatg 24780 cgatacattg atctatgtag ttttaaaca cacgcgaact ttgagaatt tatataatc 24840 attccatcga tacatccttc tatgttgaca tgtatatatc caggaattcg tttattata 24900 tcgggaatg tataaactaa aacattgccc ggaagcggtg cctctatctg cgttatatcc 24960 gttctttaact tacaaatgt aaccaatacc ttgcatgac ttgttttgtt cggcaacgtt 25020 agtttaaact tgacgaatgg attackattaca atagcatgat ccgcgcatct attaagtttt 25080 tttactttaa cgcccttgta tgttttaca gagacttat ctaatttct agtgcttgta 25140 tgtgttataa atataacggg atatagaact gaatcaccta ccttagatac ccaattacat 25200 tttatcagat ccagataata aacaaatttt gtcgccctaa ctaattctat attgttatat 25260 attttacaat tggttatgat atcatgtaat aacttggagt ctaacgcgca tcgtcgtacg 25320 tttatacaat tgtgatttag tgtagtatat ctacacatgt atttttccgc actatagtat 25380 tctggactag tgataaaact atcgttatat ctgtcttcga tgaactcatc gagatattgc 25440 tctctgtcat attcatagac ctgcataaac tttctagaca tcttacaatc cgtgttatt 25500 taggatcata tttacatatt tacgggtata tcaaagatgt tagattagtt aatgggaatc 25560 gtctataata atgaatatta aacaattata tgaggacttt taccacaaag catcataaaa 25620 atgagtcgtc gtctgattta tgttttaaat atcaaccgcg aatcaactca taaaatacaa 25680 gagaatgaaa tatatacata ttttagtcat tgcaatatag accatacttc tacagaactt 25740 gattttgtag ttaaaaacta tgatctaaac agacgacaac ctgtaactgg gtatactgca 25800 ctacactgct atttgtataa taattacttt acaaacgatg tactgaagat attattaaat 25860 catggagtgg atgtaacgat gaaaaccagt agcggacgta tgcctgttta tatattgctt 25920 actagatgtt gtaatatttc acatgatgta gtgatagata tgatagaca agtaaaaac 25980 cacttatcgc atagagacta ttcaacta ctactagagt ataaaatc tcgttacatg 26040 ttattgagg agggaat cattgagac aggtattaca ctttattaga tagggaatc 26100 gatcctaact ttaaacaaga cggatataca gcgttacatt attattat gtgtctcgca 26160 cacgtttata aaccaggtga gtgtagaaaa ccgataacga taaaaaaggc caagcgaatt 26220 atttctttgt ttatacaca tggagctaat ctaaacgcgt tagatattg tgtaataca 26280 ccattccatt tgtatcttag tattgaatg tgtaataata ttcatatgac taaaatgctg 26340 ttgactttta atccgaattt caaatatgt aaatcatg gattaacgcc tatactatgt 26400 tatataactt ccgactacat acacacgat attcttgtta tgttaataca tcactatgaa 26460 acaaatgttg gagaaatgcc gatagatgag cgtcgtataa tcgtattcga gtttatcaa 26520 acatattcta cacgtccggc agattcgata acttatttga tgaataggtt taaaaatata 26580 atattatta cccgctatga aggaagaca ttattacacg tagcatgtga atatataat 26640 acacaagtaa tagattatct tatacgtatc aacggata taatgcgtt aaccgacaat 26700 aaaaacacg ctatccaact cattatagat aaaaaaa attccccgta taccatcgat 26760 tgtttattgt atatacttag atatattgta gatagaatg tgataagatc gttggtggat 26820 caactccat ctctacctat cttcaata aaatcattg agaattcat atcctactgt 26880 atactttag atgacacatt ttacaatagg cacgttagga atccaatc taaaacgtat 26940 cgatacgcat ttcaaata catgtcgtttt gataaatacg atggtataat aactaaatgt 27000 cataagaaa caatattgct caaactatcc actgttctag acactacact atatgcagtt 27060 ttagatgcc aatattcgaa aaagttaaga agataccctca acgagttaa aaaatataat 27120 aacgataagt cctttaaaat atattctaat attgaatg agagatacct taatgtatat 27180 tataaagata tgtacgtgtc aaagtatat gataaactat ttcctgtttt cacagataaa 27240 aattgtctac taacattact accttcagaa attatatacg aaatattata catgctgaca 27300 attaacgatc tttataatat atcgtatcca cctaccaaag tatagttgta ttttctcat 27360 gcgatgtgtg taaaaaaact gatattatat aaatatttta gtgccgtata ataaagatga 27420 cgatgaaaat gatggtacat atatatttcg tatcattatc attattgtta ttgctattcc 27480 acagttacgc catagacatc gaaaatgaaa tcacagaatt cttcaataaa atgagagata 27540 ctctaccagc taaagactct aaatggttga atccagcatg tatgttcgga ggcacaatga 27600 atgatatggc cactctagga gagccattca gcgcaaagtg tcctcctatt gaagacagtc 27660 ttttatcgca cagatataaa gactatgtgg ttaaatggga aaggctagaa aagaatagac 27720 ggcgacaggt ttctaataaa cgtgttaaac atggtgattt atggatagcc aactatacat 27780 ctaaattcag taaccgtagg tatttgtgta ccgtaactac aaagaatggt gactgtgttc 27840 agggtatagt tagatctcat attagaaaac ctccttcatg cattccaaaa acatatgaac 27900 taggtactca tgataagtat ggcatagact tatactgtgg aattcttac gcaaacatt 27960 Aatadad aacttggtat aagaata aggaattta tatcgacgat attaagttatt 28020 cacaacgggg aagaaatta attackcata atccagagtt agagatagc gggaatacg 28080 actgttacgt tcattacgac gacgttagaa tcagaatga tatcgtagta tcaatgta 28140 aaatacttac gttataccg tcacagacc acaggtta actaaaaga aattgcggat 28200 atgcgtcaaa ttaataaa tggtacggac atgcttacag tgatgtacat gttaaataa 28260 cctacaaag aacgatatgt taataatccg atattacag attgggctaa taagcaat 28320 aagttttatta atcaataat atataatgct aatagttaa windowaaag windowaaata 28380 gacgacatga taggaggt atccattgac gatacgtt tatcacact accgttagaa 28440 attagacatt tgattttctc gtacgcgttc ctataaaaat agaaactata atcatataat 28500 agtgtaggtt ggtagtattg ctcttgtgac tagagactt agttaaggta ctgtaaaaat 28560 agaaactata atcatataat agtgtaggtt ggtagtaggg tactcgtgat taatttatt 28620 gttaaacttg tccttaagtc ttattaatat gtcttctaaa gggggtagtg gcggcatgtg 28680 gagtgtcttt atccatggac atgatggtag taataaagga tctaaaactt atacatctgg 28740 tggcggtgga atgtggggag gaggatcgtc cagtggggtt aacggaggtg taaaatctgg 28800 aactggtaaa atttaaacat agggataaca gggtaat 28837 <210> 9 <211> 17641 <212> DNA <213> Vaccinia virus <400> 9 tagggataac agggtaatat gacgatgaaa atgatggtac atatatattt cgtatcatta 60 tcattattgt tattgctatt ccacagttac gccatagaca tcgaaaatga aatcacagaa 120 ttcttcaata aaatgagaga tactctacca gctaaagact ctaaatggtt gaatccagca 180 tgtatgttcg gaggcacaat gaatgatatg gccactctag gagagccatt cagcgcaaag 240 tgtcctccta ttgaagacag tcttttatcg cacagatata aagactatgt ggttaaatgg 300 gaaaggctag aaaagaatag acggcgacag gtttctaata aacgtgttaa acatggtgat 360 ttatggatag ccaactatac atctaaattc agtaaccgta ggtatttgtg taccgtaact 420 acaaagaatg gtgactgtgt tcagggtata gttagatctc atattagaaa acctccttca 480 tgcattccaa aaacatatga actaggtact catgataagt atggcataga cttatactgt 540 ggaattcttt acgcaaaaca ttataataat ataacttggt ataaagataa taaggaaatt 600 aatatcgacg atattaagta ttcacaaacg ggaaagaaat taattattca taatccagag 660 ttagaagata gcggaagata cgactgttac gttcattacg acgacgttag aatcaagaat 720 gatatcgtag tatcaagatg taaaatactt acggttatac cgtcacaaga ccacaggtttt 780 aaactaaaaa gaaattgcgg atatgcgtca attaaaata aatggtacgg acatgcttac 840 agtgatgtac atgttaaata aacctacaaa gaaacgatat gttaataatc cgatatttac 900 agattgggct aataagcaat ataagtttta taatcaaata atatataatg ctaataagtt 960 atagaacaa agtaagaaaa tagacgacat gatagaggag gtatccattg acgataatcg 1020 tttatcaaca ctaccgttag aaattagaca tttgattttc tcgtacgcgt tcctataaaa 1080 atagaaacta taatcatata atagtgtagg ttggtagtat tgctcttgtg actagagact 1140 1200. 1200. 1200. 1200. 1200. ttggtagtag ggtactcgtg attaatttta ttgttaaact tgtccttaag tcttattaat atgtcttcta aagggggtag tggcggcatg tggagtgtct ttatccatgg acatgatggt agtataaag gatctaaaac ttatacatct ggtggcggtg gatgtgggg aggaggatcg tccagtgggg 1380 ttaacggagg tgtaaaatct ggaactggta aaatttaaac actaaattat ttttattaat aattgtacaa gtttttgaca tgatatttaa tgacattagt tgtgtgggtg tatagagttc acagtagctc attcagtcaa aatgtttgac tatttggaaa atgaggaggt ggctctcgat gaacttaaac agatgttgag agacagagat cctaatgata ccaggaacca attcaagaat aatgctttac acgcatacct ttttaatgag cattgtaata atgtcgaggt cgtcaaacta ctactagaca gtggcactaa tccattacgc aaaaattgga gacagctacc ccattagaag aatacacaaa tagtagcat gttaaagtta aaggatatag cgatggctct actagaagcc actggattta gcaacataaa tgactttaat atattcagct atatgaatc caaaaatgta gacgttgact tgataaggt gttggtagaa catggattg acttgagtgt taaatgtgaa 1920 aaccatcgtt cagttataga aaattatgta atgacaatga tcctgttcct aaattattg 1980 atttgttcat agaaaatggc tgcagtgttc tttatgagga cgagtactga tacgcgtatg 2040 atgattatca actacgaaat tgcggtaccg tattgcatct gtattcaag tcggatacga 2100 gagcatatgt gcgtccggaa gttgttaaat gtctaatta tcacggaatc aagccgtctt 2160 ttatagataa aaactattgt acaggtcttc atattatat taagtcatct catatagata 2220 tagacatcgt taaattgtta atgaaggaa tagatacac tgcttattca tatatagacg 2280 atctaacatg ttgcactcga ggaattatgg ctgattatct aaatagtgat tatagataca 2340 aaagatgt agatttggtc aaattgtttt tggaatgg aaagcctcac ggaataatgt 2400 gtagtattgt accactatgg agaatgata aggaaccat cttttgata ttgaaaaca 2460 tgaactcgga tgtcctccaa catatactaa ttgagtatat gatatcggc gatatccctc 2520 tagtggaata tggaactgtg gtaataag aggctataca cggatacttt agaatatta 2580 atattgattc ttacacgatg aaatatctac taaaaaagga agggagatgc catcaattat 2640 ctcgatgatg gagagatccc gattggacac ctatgtaaat ccaactatga atgttaaat 2700 ttttacactt atacatacaa aaagggtctt tgtgacatgt cttatgcttg cccaattctt 2760 agtactataa acatttgcct accttatctt aaagacatta acatgattga caaacgagga 2820 gaaacacttc ttcacaaggc tgttagatat aataaacaat ctctagtatc tttactgcta 2880 gaatccggtt cagatgtcaa cattagatca aataacggat atacatgtat agccattgcc 2940 atcaacgaat ctaaaaacat tgaactgctg aaaatgctat tatgtcataa acctacatta 3000 gattatgtga ttgattcatt gagagaaata tctaacatcg tagataacta ctatgctata 3060 aaacaatgta ttaaatatgc catgattata gatgactgta catcgtctaa gattccagag 3120 tccataagtc aacgctataa tgattatata gatctttgca attaagaatt gaatgagatg 3180 aaaaaaataa tggtaggtgg tatactatg ttctcattaa tatttactga tcatggagct 3240 aaaattattc atagatatgc caataatcca gaattacgtg agtattatga gttaaaacaa 3300 aataaaatat atgtggaagc atatgatatt atttccaacg caatagtgaa acatgataga 3360 atacataaaa ccatagaatc agttgatgat aatacctaca tttctaatct tccgtatacc 3420 atcaaataca aaatattcga gcaacaataa gtatttttta tacctttaaa attgataaat 3480 aaatttttc tagtgatatt ttggcaagat gagaatccta tttctcatcg ccttcatgta 3540 tgggtgtgtt cacccatatg ttaatgccga cgaaatcaaa tgtcctaacc taaacatcgt 3600 aacatcttct ggagaatttc gttgcactgg atgtgtgaaa tttatgccta attttagcta 3660 catgtattgg ttggcaaagg atatgagatc ggacgaggat accaagttta tagaacatct 3720 gggtgatggc atcaaagaag atgaaaccgt tcgtaccata gatggtagaa tcgtcactct 3780 acagaaagtc cttcatgtaa ccgatactaa taaatttgct cattataggt tcacttgtgt 3840 cctcactacg atagatggca tttataaaaa gaatgtttgc tgaagtagtt gtgtgctact 3900 atttttatt atgatataat ctaaatgcaa ttgtcttgag aatccatcca atacggcaga 3960 ttatattaga aacaacgaat tgaattgaat ttaatctttc atacgagaaa aataacgaca 4020 tacgtcgtca acaaattaaa ctttttattt attagttaac tagcttatag aacttgctca 4080 ttgttatgtt tctaaaacgg gtacgacata taggacaatt atccgacgca ccggtttctc 4140 ttcgtgtttt atgccatata ttgatacatg ttatgcaaaa tatatgatta cacgaatcca 4200 ataaaccaaa gtatctatcg ttttgagtaa acaacttcat agcaaattcc acattcttt 4260 tctttactta ctctatacac gtcctcgtat ttatccagta ttttgatgat atccaactca 4320 gaaatggttg ttgtattatt gggtgttatag gtattattag ctatgtacca atttaccaac 4380 cttcttaata ttgattgata atcacatcgg ttatccaatt aataactaaa ttgtagtgta 4440 tatatagacc atatatgttt ctatttttt gacagttacg tatagtttca gtaagtttg 4500 attgttgtat tcctgtatct ctagataagt tagtcatata gtcccttccg gcgatacgtt 4560 ttttccaagc ccgaaattga ttagccaaat gtggatttat ttttgtgata ttgatataat 4620 atttcggata atgcatactg ttagtcttat atcatttggt tcatctatgt attgtaatat 4680 tgttacatga tctatagatag atgtattgat tttggcagga tcgaattcca tatccgcgac 4740 taaacagtga aaaaatgta atacttttt aaatttttaaa tgtaaac tttttttat 4800 tttttatgat tccaaaaata ctgaataca agtcctaaat tataaatatg gagatcatac 4860 taccacact tattattag tattacaggc cggtgtatata tattagatata tataattcta 4920 ttacaccggc agacaattac cgatcggtat tgtcgttac icacataccg tataatatgt 4980 atatacaat tccataaccc attgacagtt gttatacatc aaaattgcaa ttctttgat 5040 tacgatgtta tagaatgta gttaattgat gtatgatgtt aatgtgtcct ctttccctctt 5100 atacatcgt aatcaaaac ttttatta tatataccta ataatgtgtc ttaatagttc 5160 tcgtgattcg tcaacaatc attcttataa atataataa atcaacgtaa aaacacataa 5220 aaataagcgt actaataag acatgata tttacgacga taaggtcta cagactatta 5280 aactgtttaa taatgaattt gattgtataa ggatgacat cagagaatta tttaaacatg 5340 taactgattc cgatagtata caacttccga tggaagacaa ttctgattt atagaaata 5400 tcagaaaaat actatataga cgattaaaaa atgtagaatg tgttgacatc gataacacaa 5460 taacttttat gaaatacgat ccaaatgatg samaaagcg tacgtgttct aattgggtac 5520 ccttaactaa taactatatg gaatattgtc tagtaatata tttggaaaca ccgatatgtg 5580 gaggcaaaat aaaattatac caccctacag gaataataa gtcggataag gatattatgt 5640 ttgcaaagac tctagacttt aaatcaaaga aagtgttaac tggacgtaag acaattgccg 5700 ttctagacat atccgtttca tataatagat caataactac tattcactac aacgacgacg 5760 5820 5880 aatgtctgtt agtaataac catctaggta taggtatcgt taaagataaa cgtataagcg 5940 atagtttgg agatgtatgt atggatacaa tatttgactt ttctgaagca cgagagttat 6000 tttcattaac taatgatgat aacagaaata tagcatggga cactgataaa ctagacgatg 6060 atacagatat atggactccc gtcacagaag atgattacaa atttctttct agacttagtat 6120 tgtatgcaaa atctcaatcg gatactgtat tcgactatta tgttcttact ggtgatacgg 6180 aaccaccac tgtattcatt ttcaggtaa ctagatttta ctttaatatg ctgaaataaa 6240 aaatttttgt atatatcta gaggtagagg tattgtttag ataataca atacataga 6300 tacatcgcat atttagcatt tttataaata tacataagac atacacttta tacatttttg 6360 taaaaatact cataaaaaaa atttaaaa attatggcac aaccatatct tgtagaggta 6420 gtttagttcg tcgagtgaac ctataaacag atatagaca acacataata ataatgccta 6480 ctaatacaag catataccg ggagatggga tatatgacgt tgtagtgttt ggttttctg 6540 aacgttgata gtctactaat actacatgct gatactaat gcctgtataa ccatgagagc 6600 atctacaata cataccgtcg atatctctag cgtggataca gtcaccgtgt aaacaatatc 6660 catctccctc tggaccgcat aatctgatag ctggaatatc tgttgtagcg tttgtaattt 6720 ctggcaatgt cgtttcgata gcgttaccac tatcggcgaa tgatctgat atcatagcag 6780 cgaacaacaa catcagatat ttcatcgaca ttttgatgg attctgtgtt tatgctgttt 6840 ctcagtgtgt gtttatgaca agattgggaa ttttatta ttaattcagt atataact 6900 aataatatat tgttaattgt gtaaataata taaaaataac aatacaatat tgaatgtgtt 6960 gctgttaaaa atgtatgtgt tataataa gaataaaata aatgagtatg atcattttag 7020 ataacgattg attttatcat tacccttcat tcttatattc tttgcttacg gaacctatat 7080 ttagaaacat ctactaacga ttttttatgc ttgcattatt aatggtatgt aataccaatt 7140 tgttaagtat gaatacgggg tacaaacata aactgaaatt taacattatt tattatgat 7200 atatatcgtt atcgttattg tttggtctat accatggata tctttaaaga actaatcgta 7260 aaacaccctg atgaaaatgt tttgatttct ccagtttcca tttatctac tttatctatt 7320 ctaaatcatg gagcagctgg ttctacagct gaacaactat caaaatatat agagaatatg 7380 aatgagaata cacccgatga taagaaggat gacaataatg acatggacgt agatattccg 7440 tattgtgcga cactagctac cgcaaataaa atatacggta gcgatagtat cgagttccat 7500 gcctccttcc tacaaaaaat aaaagacgat tttcaaactg taaactttaa taatgctaac 7560 caaacaaagg aactaatcaa cgaatgggtt aagacaatga caaatggtaa aattaattcc 7620 ttattgacta gtccgctatc cattaatact cgtatgacag ttgttagcgc cgtcctttt 7680 aaagcaatgt ggaaatatcc attttctaaa catcttacat atacagacaa gtttatatt 7740 tctaagaata tagttaccag cgttgatatg atggtgggta ccgagaataa cttgcaatat 7800 gtacatatta atgaattat cgaggattc tctattatcg atattccata cgagggaaac 7860 tctagtatgg tgattatact accggacgac atagaaggta tatataacat agaaaaaat 7920 ataacagatg aaaaatttaa aaaatggtgt ggtatgttat ctactaaaag tatagacttg 7980 tatatgccaa agtttaaagt ggaaatgaca gaaccgtata atctggtacc tattctagaa 8040 aatttaggac ttactaatat attcggatat tatgcagatt ttagcaagat gtgtaatgaa 8100 actatcactg tagaaaaatt tctacatacg acgtttatag atgttaatga ggagtataca 8160 gaagcatcgg ccgttacagg agtatttatg actaactttt cgatggtata tcgtacgaag 8220 gtctacataa accatccatt catgtacatg attaaagaca acacaggacg tatacttttt 8280 atagggaaat actgctatcc gcaataaata taaacaaata gacttttatc acgtttatct 8340 atgtctaaat attacaaata gtataaacta aagctgataa tacttaaaaa aataataata 8400 tcatttacaa ttaatatagtat aaactaaaaa ttaaacaaat cgttattata agtaatatca 8460 aaatgatgat atacggatta atagcgtgtc ttatattcgt gacttcatcc atcgctagtc 8520 cactttatat tcccgttatt ccacccatta cggaagataa atcgttcaat agtgtagagg 8580 tattagtttc cttgtttaga gatgaccaaa aagactatac ggtaacttct cagttcaata 8640 actacactat cgataccaaa gactggacta tcggcgtact atccacacct gatggtttgg 8700 atataccatt gactaatata acttattggt cacggtttac tataggtcgt gcattgttca 8760 aatcagagtc tgaggatatt ttccaaaaga aaatgagtat tctaggtgtt tctatagaat 8820 gtaagaagtc gtcgacatta cttacttttt tgaccgtgcg taaaatgact cgagtattta 8880 ataaatttcc agatatggct tattatcgag gagactgttt aaaagccgtt tatgtaacaa 8940 tgacttataa aatactaaa actggagaga ctgattacac gtacctctct aatggggggt 9000 tgcctgcata ctatcgtaat ggggtcgatg gttgattatt gattagtata ttccttattc 9060 acacaaaag aacatttta taaacatgaa accactgtct aaatgtatt atgatcttga 9120 tttatagatg aagatcagcc tttagaggat ttataccagt atgtttaata tgaaaaaaat 9180 aaacataaca tattttgaga ttaagcgcta ttgtgcttaa ttattgct ctataaactg 9240 atatatagc cacaattatt gacggggctg ttatgaccg gcaatcatga attacagaa 9300 attatctctg gctatatatc ttactgcgac atgttcgtgg tgttatgaaa catgcataag 9360 aaaaactgcg ttgtatcatg acattcaatt ggagcatgta gagacaata agatagtgt 9420 agcgtcgcta ccgtacaagt agtcaatcaa agagaacgta gtagattgtt ggctacattt 9480 aattggacag atagctga gggtgttaga atgagttca ttaaaatatg tgatatcac 9540 ggaacatatt tatatta tactattgct gttagtataa ttattgattc cacggaagaa 9600 ctaccacag ttactccaat tacaaca tatgaacctt ctacatataa ttatactc 9660 gatgatagca ctgttattac tactgaagaa ctacaagtga ctcctcatat ggatctccat 9720 cgatgataca tgtattaaaa tactttccga ataagtcttt taaatattgt attaattatg 9780 aaaaaactatg ctatgcgagt atgatgcaaa gatgtttaat gatacgatac tagattttat 9840 ctctagcgag agatgtcgtt agaatcattt atcatacta cgtttaataa taattcatca 9900 acgaatatcg ataacatgtg tcatttatac gttaaagtct gtccgtcttc tctattgttt 9960 agactgtttg tagaatgctg tgatataaac aaactagtag aaggtacgac tccgttacac 10020 tgttatctaa tgaatgaagg atttgaatca tctgttttaa aaaacctatt aaaggagtat 10080 gtcatgacag tgatggtcac attccattac acaaatattt aactcatgat gaagttgaga 10140 atgatatgct ttctgatagt atagatagct ttagctaata taaaaatata ttaatccact 10200 atatattcta gacttgattt aaaaccgata aactactacg tactgtataa gttgttaaga 10260 aaaggagcag accctaatta tgcagatgat agaggtaata cttttcttca ttacttctgc 10320 atctatatgt ccacttatga gaaaacgtca tttaataaga tgcatcgtga aaagaaattt 10380 attaaagagt tggtaaaata tgaaaccgaa agtaaataat ataggaaata cacctctaca 10440 taactacgta tctcaatatg atatcactct cattcctcat ccacaaccca ttaaaaaaaat 10500 ggaaattaaa gccctctatt agcataaacg gctacaggtc tacctttaca atggcctttc 10560 cttgtgccca gttcagaccc tgtcattgcc acgctactaa ggactccctg aataccgtgg 10620 ccgacgtcag acattgtctg actgaataca tcctgtgggt ttctcataga tggacccata 10680 gagaaactgc agggcctctc tacaggcttc tcatctcttt cagaactgat gcaacggagc 10740 tctttggtgg tgagttgaag gattcacttc cgtgggacaa tatcgacaat tgcgtggaga 10800 tcattaaatg tttcatcaga aatgactcca tgaaaaccgc cgaagaactt cgtgcaatca 10860 ttggactttg tactcaatca gctatcgtct ctggaagagt cttcaacgat aagtatatcg 10920 acatactact tatgctgcga aagattctga acgagaacga ctatctcacc ctcttggatc 10980 atatccgcac tgctaaatac taaatctcct tcatgctctc tcactacact ttttatcatc 11040 ttatgaggaa tgattgcctt catcattttt cgtgaaatag gaataattag caccagaata 11100 gctatggatt gcacatgtat tctatgtcgt ctactggatg aagatgtgac gtacaaaaaa 11160 aaaactag aaattgaaac gtgtcacaac ttatcaaac atagatag acgaggaac 11220 aatgcgctac attgttacgt cttcataaa tgcgatacag acatgat tgttcgactg 11280 ttactctc gcggagtcga gagactttgt agaacaacg aaggattaac tccgcttagga 11340 gtatacagta agcatagata cgtcaaatct caattgtgc atctactgat atccagctat 11400 tcgaattcct ctaacgaact caagtcgaat ataatgatt tcgatctgta ttcggataat 11460 atcgacttac gtctgctaaa atacctatt gtggataac ggatacgtcc gtccaagaat 11520 acgaattatg caatcaatgg tctcggattg gtggatatat acgtaacgac gcctaatccg 11580 agaccagaag tattgctatg gcttcttaa tcagaatgtt acagcaccgg ttacgtattt 11640 cgtacctgta tgtacacag tgatatgtgt aagaactctc ttcattacta tatatcgtct 11700 catagagaat ctcaatctct atccaaggat gtattaat gtttgatcaa taacaatgtt 11760 tccatccatg gcagagacga aggagatct tccaccatcc atactactg gtctttctca 11820 accatagata tagattgt taaattatta tataaagg atgtggacac gtgtagagta 11880 tacgacgtca gccctatatt agaggcgtat tatctaaaca agcgatttag agtaacccca 11940 tataatgtag acatggaaat cgttaatctt cttattgaga gacgtcatac tcttgtcgac 12000 gtaatgcgta gtattacttc gtacgattcc agagaatata accactacat catcgataac 12060 attctaaaga gatttagaca acaggatgaa tccatcgtac aagccatgtt gataaactac 12120 ttacattacg gcgatatggt cgttcgatgc atgttagata acggacaaca actatcctct 12180 gcacgactac tttgttaata ataatctcgt cgatgtaaac gtcgtaaggt ttatcgtgga 12240 aaatatggac acgcggctgt aaatcacata tctaacaatg gccgtctatg tatgtacggt 12300 ctgatattat cgagatttaa taattgcggg tatcactgtt atgaaaccat actaatagat 12360 gtatttgata tactaagcaa gtacatggat aatagata tgatcgataa tgagaataaa 12420 actctactat attacgcggt cgatgtcaat aatatacaat ttgcaaagcg gttatggaa 12480 tatggagcga gtgttacaac atcacgctcg ataatcaata cggccatcca gaaagtagt 12540 tacagagag aaaaaaaac gaagctagtt gatttac tagctacca tcccactcta 12600 gagactatga ttgacgcatt taatagagat atacgctatc tatatcctga accattattc 12660 gcctgtatca gatacgcctt atcatagat gatgatttc ctctaaagt aaagtatgat 12720 atcgccggtc gtcatagga actaaagcgc tatagagtag acattaatag atgaagaat 12780 gcctacatat caggcgtctc catgttttgat atattatta aacgaagcaa acgccacaga 12840 ttgagatacg siaagaatcc gatcaat gggtacaaaa agaactaacg tccatcatta 12900 cagaaactgt aaagaacaat gagaggatcg actccatagt ggacaactt atacagacg 12960 ataacttgat ttcgaatta cccatggaga tactttatta ctccattaaa taatttatca 13020 tggagcgata atgtcctgtt tcattgttt ccatgacata ttacaaatc gattccgtcc 13080 aagatgataa aaacatttac cggcatcata aacacggagt ttttatta tgtctcgcat 13140 aaacattact aaaaaaatat attgttcggt ttcttcac atctttaatt atgaaaagt 13200 aaatcattat gagatggacg agattgtacg catcgttcgc gagagtatgt ggtacatacc 13260 taacgtattt atggacgacg gtaagaatga aggtcacgtt tctgtcaaca atgtttgtca 13320 tatgtatttt acgttctttg atgtggatac atcgtctcat ctgtttaagc tagttattaa 13380 acactgcgat ctgaataaac gaggtaactc tccattacat tgctatacga tgaatacacg 13440 atttaatcca tctgtattaa agatattgtt acaccacggc atgcgtaact ttgatagcaa 13500 ggatgaccac tatcaatcga taacaagatc tttgatatac taacggacac cattgatgac 13560 tttagtaaat catccgatct attgctgtgt tatcttagat ataaattcaa tgggagctta 13620 aactattacg ttctgtacaa aggatccgac cctaattgcg ccgacgagga tgaactcact 13680 tctcttcatt actactgtaa acacatatcc acgttctaca aaagcaatta ttacaagtca 13740 agtcacacta agatgcgagc cgagaagcga ttcatctacg cgataataga ttatggagca 13800 aacattaacg ccgttacaca cttaccttca acagtatacc aaacatagtc ctcgtgtggt 13860 gtatgctctt ttatctcgag gatacgtaat aatcttgatt gtacacccat catggaacga 13920 ttgtgcaaca ggtcatattc tcataatgtt actcaattgg cacgaacaaa aggaagaagg 13980 acaacatcta ctttatctat tcataaaca taatcaagga tacactctca atatactacg 14040 gtatctacta gataggttcg acattcagaa agacgaatat tataataccg cctttcaaaa 14100 ttgtaacaac aatgttgcct catacatcgg atacgacatc aaccttccga ctaaagacgg 14160 tattcgactt ggtgtttgaa aacagaaaca tcatatacaa ggcggatgtt gtgaatgaca 14220 tcatccacca cagactgaaa gtacctatga ttaaatcgtt gttctacaag atgtcagaat 14280 tctctcccta cgacgattac tacgtaaaaa agatactagc ctactgccta ttaagggacg 14340 agtcattcgc ggaactacat agtaaattct gtttaaacga ggactataaa agtgtattta 14400 tgaaaaatat atcattcgat aagatagatt ccatcatcgt gacataagtc gccttaaaga 14460 gattcgaatc tccgacaccg acctgtatac ggtatcacag ctatcttaaa gccacatatt 14520 cagacagtca catttcattt cccatgtacg acgatctcat agaacagtgc catctatcga 14580 tggagcgtaa aagtaaactc gtcgacaaag cactcaataa attagagtct accatcgatg 14640 gccaatctag actatcgtat ttgcctccgg aaattatgcg caatcatc taaacagtat 14700 gttgtacgga aagaaccatt acaattatta tccatgatag aaagaaaata tctatatgat 14760 tggagaagta ggaaacagga acagacac gattactaca tattaattt atgaagtccg 14820 tattatactc gtatattattg tttctcat gtataataat aaacggaaga gatatagcac 14880 cgcatgcacc atccgatgga aagtgtaag acaacgaata caacgccat aatttgtgtc 14940 cgggaacata cgcttccaga ttagcgata gcaagactaa cacacaatgt acgccgtgtg 15000 gttcgggtac cttcacatct cgcaataatc atttacccgc tgtctaagt tgtaacggaa 15060 gatgcgatcg tgtacacta ctcacaatag aatctgtgaa tgctctcccg gatattattg 15120 tcttctcaa ggatcatccg gatgcaaggc atgtgttttcc caacaaat gtggaatagg 15180 atacggagta tccggacg tcatctgttc tccgtgtggt ctcggaacat attctcacac 15240 cgtctcttcc gcagataaat gcgaacccgt acccagaat acgttttaact atatcgatgt 15300 ggaaattaac ctgtatccag ttacgacac atcgtgtact cggacgacca ctaccggtct 15360 cagcgaatcc atctcaacgt cggactaac tattactatg atcataag actgcgatcc 15420 cgtctttcgt gatggatact tctctgtcct taataggta gcaacttcag gttctttac 15480 aggaaagg tgtgcactct gatttcgag attaaatgca atacaaaga ttctcctcc 15540 aaacagttaa cgaaagcaa gatgatact atcatgccgc attcggagac tgtctatcta 15600 gcgtcgacat ctatatacta tatatata ccaatactca agactacgaa actgataca 15660 tctcttatca tgtgggtaat gttctcgatg tcgatagcca tatgcccggt agttgcgata 15720 tacataaact gatcactaat tccaaaccca cccgcttttt atagtaagtt tttcaccat 15780 aaatacaata attaatttct cgtaaaagta gaaatatat tctaatttat tgcacggtaa 15840 ggagtagaa tcataagaa cagtaatcaa tcatgaaaca atatatcgtc 15900 ctggcatgca tgtgcctgcc agtcttcagc aatcctcctc ctcgtgtacg gagaagaaa 15960 aaacatca tattgggaatc gatgttatta tcaagtcac aaaaaaacac tornado 16020 ccaatgataa gatttgccaa tccgtaacgg aaattacaga gtccgagtca gatccagatc 16080 ccgaggtgga atcagaagat gattccacat cagtcgagga tgtagatcct cctaccactt 16140 attactccat catcggtgga ggtctgagaa tgaactttgg attcaccaaa tgtcctcaga 16200 ttaaatccat ctcagaatcc gctgatggaa acacagtgaa tgctagattg tccagcgtgt 16260 ccccaggaca aggtaaggac tctcccgga tcactcatga agaagctctt gctatgatca 16320 aagactgtga ggtgtctatc gacatcagat gtagcgaaga agaaagac agcgacatca 16380 agacccatcc agtactcggg tctaacatct ctcataagaa agtgagttac gaagatatca 16440 tcggttcaac gatcgtcgat acaaaatgcg tcaagaatct agagtttagc gttcgtatcg 16500 gagacatgtg caaggaatca tctgaacttg aggtcaagga tggattcaag tatgtcgacg 16560 gatcggcatc tgaaggtgca accgatgata cttcactcat cgattcaaca aaactcaaag 16620 cgtgtgtctg aatcgataac tctattcatc tgaaattgga tgagtagggt taatcgaacg 16680 attcaggcac accacgaatt aaaaaagtgt accggacact atattccggt ttgcaaaaca 16740 aaaatgttct taactacatt cacaaaaagt tacctctcgc gacttcttct ttttctgtct 16800 caatagtgtg atacgattat gacactattc ctattcctat tcctatttcc tttcagggta 16860 tcacaaaaat attaaacctc tttctgatgg tctcataaaa aaagttttac aaaaatattt 16920 ttattctctt tctctctttg atggtctcat aaaaaaagtt ttacaaaaat atttttattc 16980 tctttctctc tttgatggtc tcataaaaaa agttttacaa aaatattttt attctctttc 17040 tctctttgat ggtctcataa aaaaagtttt acaaaaatat ttttattctc tttctctctt 17100 tgatggtctc ataaaaaaag ttttacaaaa atatttttat tctctttctc tctttgatgg 17160 tctcataaaa aaagttttac aaaaatattt ttattctctt tctctctttg atggtctcat 17220 aaaaaaagtt ttacaaaaat atttttattc tctttctctc tttgatggtc tcataaaaaa 17280 agttttacaa aaatattttt attctctttc tctctttgat ggtctcataa aaaatattaa 17340 acctctttct gatggtgtca ctaaaatatt tttattctca ttctcatttt ctctttctct 17400 cttcaatgga gtcataaaat atttttattc tctttctctc ttcgatggtc tcacaaaaat 17460 attaaacctc tttctgatgg tgtcactaaa atatttttat tctcattctc attttctctt 17520 tctctcttca atggagtcat aaaatatttt tattctcttt ctctcttcga tggtctcaca 17580 aaaatattaa acctcttgct agcttctggc gaagagcttc ttctagggat aacagggtaa 17640 t 17641 <210> 10 <211> 2540 <212> DNA <213> Vaccinia virus <400> 10 tagggataac agggtaatga agaagctctt cgccatcaga aagaggttta atatttttgt 60 gagaccatcg aagagagaaa gagataaaac ttttttacga ctccatcaga aagaggttta 120 atatttttgt gagaccatcg aagagagaaa gagataaaac ttttttacga ctccatcaga 180 aagaggttta atatttttgt gagaccatcg aaggagaaag agataaaact tttttacgac 240 tccatcagaa agaggtttaa tatttttgtg agaccatcga aggagaaaga gataaaactt 300 ttttacgact ccatcagaaa gaggtttaat atttttgtga gaccatcgaa gagagaaaga 360 gataaaactt ttttacgact ccatcagaaa gaggtttaat attttgtga gaccatcgaa 420 gaggaaaga gataaaactt ttttacgact ccatcagaaa gaggtttaat attttgtga 480 gaccatcgaa ggagaaagag ataaacttt tttacgactc catcagaaag aggtttaata 540 tttttgtgag accatcgaag agaaagag ataaaacttt tttacgactc catcagaaag 600 aggtttaata tttttgtgag accatcgaag agaaagag ataaaacttt tttacgactc 660 catcagaaag aggtttaata tttttgtgag accatcgaag agaaagag ataaaacttt 720 tttacgactc catcagaaag aggtttaata tttttgtgag accatcgaag agaaagag 780 ataaacttt tttacgactc catcagaaag aggtttaata tttttgtgag accatcgaag 840 agaaagag ataaaacttt tttacgactc catcagaaag aggtttaata tttttgtgag 900 accatcgaag agaaagag aaagatag ttgatctaga tatttttctt agtacaaaag 960 tcaatgtttt aaaatattg gacaagaatt tgtctgtata aaaacttgtg tgaaattttg 1020 taccaaagaa aaaatgtgag foottcccc tacatggatt ttactagatc atttatatac 1080 caaaaaatat tatacgatct acgttttatt atatgatttt aacgtgtaaa ttataaacat 1140 tattttatga tatacaattg tctggtaacc tagatgggca taggggatgt tgataagctc 1200 gacgagtata tgttgttgga cgttattgtt taagaaatag ttgatgcatc agaaagaagaa 1260 taaaaaatat tttagtgaga ccatcgaaga gagaaagaga taaaaactttt ttacgactcc 1320 atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga gagaaaga taaaactttt 1380 ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga gagaaagaga 1440 taaaactttt ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga 1500 gagaaagaga taaaactttt ttacgactcc atcagaaaga ggtttaatat ttttgtgaga 1560 ccatcgaaga gagaaagaga taaaactttt ttacgactcc atcagaaaga ggtttaatat 1620 ttttgtgaga ccatcgaaga gagaaagaga taaaactttt ttacgactcc atcagaaaga 1680 ggtttaatat ttttgtgaga ccatcgaaga gagaaaga taaaactttt ttacgactcc 1740 atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga gagaaaga taaaactttt 1800 ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga gagaaagaga 1860 taaaactttt ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga 1920 gagaaagaga taaaactttt ttacgactcc atcagaaaga ggtttaatat ttttgtgaga 1980 ccatcgaaga gagaaagaga taaaactttt ttacgactcc atcagaaaga ggtttaatat 2040 ttttgtgaga ccatcgaaga gagaaagaga taaaactttt ttacgactcc atcagaaaga 2100 ggtttaatat ttttgtgaga ccatcgaaga gagaaaga taaaactttt ttacgactcc 2160 atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga gagaaaga taaaactttt 2220 ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga gagaaagaga 2280 taaaactttt ttacgactcc atcagaaaga ggtttaatat ttttgtgaga ccatcgaaga 2340 gagaaagaga taaaactttt ttacgactcc atcagaaaga ggtttaatat ttttgtgaga 2400 ccatcgaaga gagaaagaga taaaactttt ttacgactcc atcagaaaga ggtttaatat 2460 ttttgtgaga ccatcgaaga gagaaagaga taaaactttt ttacgactcc gctagcgaag 2520 aatagggata acagggtaat 2540 <210> 11 <211> 61 <212> DNA <213> Vaccinia virus <400> 11 tgtgtgaccc atgactgtag gaaactctag agtgtaagaa agatcgatcg ctttatagag 60 a 61 <210> 12 <211> 61 <212> DNA <213> Vaccinia virus <400> 12 tggtctctat aaagcgatcg atctttctta cactctagag tttcctacag tcatgggtca 60 c 61 <210> 13 <211> 157 <212> DNA <213> Vaccinia virus <400> 13 acattttttt ctagacacta aataaaatat ttaaaatat atattaatgt actaaaactt 60 atatattatt aatttatcta actaaagtta gtaaattata tatataattt tataattaat 120 ttaattttac taattttatt tagtgtctag aaaaaaa 157 <210> 14 <211> 157 <212> DNA <213> Vaccinia virus <400> 14 acattttttt ctagacacta aataaaatta gtaaaattaa attaattata aaattatata 60 tataatttac taactttagt tagataaatt aataatatat aagttttagt acattaatat 120 tatattttaa atattttatt tagtgtctag aaaaaaa 157 <210> 15 <211> 58 <212> DNA <213> Vaccinia virus <400> 15 gtgacccatg actgtaggaa actctagagt gtaagaaaga tcgatcgctt tatagaga 58 <210> 16 <211> 58 <212> DNA <213> Vaccinia virus <400> 16 gtgaccaacg accgtaggaa actctagagg gtaagaaaaa tcaatcgctt tatagaga 58 <210> 17 <211> 58 <212> DNA <213> Vaccinia virus <400> 17 gtgaccaacg accgtaggaa actctagagg gtaagaaaaa tcaatcgctt tatagaga 58 <210> 18 <211> 58 <212> DNA <213> Vaccinia virus <400> 18 gtgaccaacg accgtaggaa actctagagg gtaagaaaaa tcaatcgctt tatagaga 58 <210> 19 <211> 154 <212> DNA <213> Vaccinia virus <400> 19 acattttttt ctagacacta aataaatatt taaaatataa tattaatata ctaaaattta 60 tgtattatta atttatctaa ctaaagttag taaattatat acataatttt ataattaatt 120 taattttact atttatttag tgtctagaaa aaaa 154 <210> 20 <211> 154 <212> DNA <213> Vaccinia virus <400> 20 acattttttt ctagacacta aataaatagt aaaattaaat taattataaa attatgtata 60 taatttacta actttagtta gataaattaa taatacataa attttagtat attaatatta 120 tattttaaat atttatttag tgtctagaaa aaaa 154 <210> 21 <211> 61 <212> DNA <213> Vaccinia virus <400> 21 tgtgtgacca acgaccgtag gaaactctag agggtaagaa aaatcaatcg ctttatagag 60 a 61 <210> 22 <211> 61 <212> DNA <213> Vaccinia virus <400> 22 tggtctctat aaagcgattg atttttctta ccctctagag tttcctacgg tcgttggtca 60 c 61 Brief description of the drawings

[0024] This patent application contains at least one drawing executed in color. Copies of this patent application with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

[0025] The above summary of the invention and the following detailed description will be better understood when read in conjunction with the accompanying drawings. For the purpose of illustrating the invention, there are shown in the accompanying drawings presently preferred embodiments. However, it should be understood that the invention is not limited to the precise arrangements and instrumentalities shown.

[0026] Figure 1A and 1B . Schematic representation of the linear dsDNA VACV genome of strain ACAM2000 (Genbank accession number AY313847). Figure 1A The unmodified genomic sequence of the VACV ACAM2000 genome is elucidated, showing the naturally occurring AarI and BsaI restriction sites. Figure 1B Depicts the modified VACV ACAM2000 genome used for chemical synthesis of large dsDNA fragments. Overlapping scVACV ACAM2000 genome fragments are depicted in blue. Also shown are engineered BsaI restriction sites that were not silently mutated in the left inverted terminal repeat (LITR) and right inverted terminal repeat (RITR).

[0027] Figures 2A-2C Detailed schematic representation of the first ~1500-3000 bp of the published genomes of (A) VACV WR strain and (B) VACV ACAM2000. Tandem repeat regions are indicated by red (70 bp repeats), blue (125 bp repeats), and green (54 bp repeats) boxes. The ORF corresponding to gene C23L is also indicated in each genome. (C) Schematic representation of the direct repeat region containing the 70 bp repeats in VACV WR. This sequence was synthesized to contain a SapI restriction site at the 5' end and an NheI restriction site at the 3' end for ligating the hairpin / duplex fragment and the VACV ACAM2000 ITR fragment, respectively.

[0028] Figure 3A and 3B Vaccinia virus terminal hairpin loops were assembled with duplex DNA to the first 70 bp repeat. (A) Depicts the ordered sequence of phosphorylated oligonucleotides used to generate WR duplex DNA. (B) Depicts gel electrophoresis of WR strain duplex DNA (lane 2) and hairpin DNA alone (lane 3) and after ligation (lane 4). The ligation product (arrow) was then excised from the gel and purified, enabling ligation to the 70 bp repeat to mimic the sequence of the wtVACV ACAM2000 sequence.

[0029] Figure 4 The SapI / NheI-digested 70bp repeat fragment was ligated with the WR strain hairpin / duplex DNA fragment. The 70bp repeat fragment was digested with SapI and NheI, gel purified, and then ligated with the hairpin / duplex DNA fragment at a molar ratio of 5:1 hairpin / duplex DNA:70bp fragment. An upward shift in the approximately 2300bp band in lanes 4 and 5 indicated successful addition of the hairpin / duplex fragment. These bands were then gel-extracted from the gel and ligated to the digested VACVACAM2000 ITR fragment.

[0030] Figure 5 Digestion of the scVACV ACAM2000 fragment. The ITR fragment was digested with both NheI and I-SceI at 37°C for 2 hours, followed by dephosphorylation with alkaline phosphatase to remove phosphate groups and facilitate more efficient ligation of the fragment to the terminal hairpin loop / duplex / 70 bp tandem repeat. The other scVACV ACAM2000 DNA plasmid was linearized with I-SceI at 37°C for 2 hours, followed by heat inactivation of the restriction enzyme at 65°C for 10 minutes.

[0031] Figure 6 In vitro growth properties of .scVACV ACAM2000-WR DUP / HP. Multistep growth kinetics were measured in monkey kidney epithelial cells (BSC-40). Cells were infected at a multiplicity of infection of 0.03, virus was harvested at the indicated times, and virus was titrated on BSC-40 cells. Data represent three independent experiments. Error bars represent standard error of the mean (SEM).

[0032] Figure 7 In vitro growth properties of scVACV ACAM2000-WR DUP / HP and scVACV ACAM2000-ACAM2000 DUP / HP compared to scVACV ACAM2000-WR DUP / HP and scVACV ACAM2000-ACAM2000 DUP / HP in which the YFP-gpt marker has been replaced with the J2R gene sequence (VAC_WRΔJ2R), as well as wtVACV ACAM2000. Multistep growth kinetics were measured in monkey kidney epithelial cells (BSC-40). Cells were infected at a multiplicity of infection of 0.03, virus was harvested at the indicated times, and virus was titrated on BSC-40 cells. Error bars represent standard error of the mean (SEM).

[0033] Figure 8Restriction enzyme mapping of reactivated scVACV ACAM2000-WR DUP / HP clones. Pulsed-field gel electrophoresis analysis. Two independent scVACV ACAM2000-WR DUP / HP clones plus a VACV WR control (in which the YFP-gpt marker had been replaced with the J2R gene sequence (VAC_WRΔJ2R)) and a wtVACV ACAM2000 control (VAC_ACAM2000) were purified and then left undigested, digested with BsaI, HindIII, or NotI and PvuI. The expected deletion of almost all BsaI sites in the scVACV ACAM2000 clones was evident. Minor differences were observed in HindIII-digested scVACV ACAM2000 genomic DNA compared to VAC_WRΔJ2R and VACV_ACAM2000. Digestion of genomic DNA with NotI and PvuI cleaves the 70 bp tandem repeats found at the left and right ITR sequences. In VAC_WRΔJ2R, the approximate size of the 70 bp repeat sequence is close to 3.6 kbp. Interestingly, two bands of different sizes corresponding to the 70 bp tandem repeat (marked with *) were observed in two independent scVACV ACAM2000 clones, even though the full-length 70 bp tandem repeat element was linked to the ITR segment. When ACAM2000 genomic DNA was digested with NotI and PvuI, a band at ~4.7 kbp was observed, which may indicate the size of the 70 bp repeat sequence in ACAM2000.

[0034] Figure 9 Nucleotide sequence variations among VACV strain sequences. Figure 9 A depicts VACV nucleotide sequence variations within the duplex region in the ITRs (SEQ ID NOs: 15-18). Figure 9 B depicts the VACV ACAM2000 secondary hairpin loop, which is covalently linked to the end of the linear dsDNA genome of ACAM2000 (S form SEQ ID NO: 19 and F form SEQ ID NO: 20). The terminal loop sequence is highlighted in green. Detailed Description of the Invention

[0036] General techniques

[0037] Unless otherwise defined herein, the scientific and technical terms used in this application should have the implication that those of ordinary skill in the art usually understand. Usually, the nomenclature used in combination with the pharmacology, cell and tissue culture, molecular biology, cell and cancer biology, neurobiology, neurochemistry, virology, immunology, microbiology, genetics and protein and nucleic acid chemistry described in the present invention and its technology are those well known in the art and commonly used. In the case of conflict, this specification (including definition) shall prevail.

[0038] The practice of the present invention will employ, unless otherwise indicated, conventional techniques of molecular biology (including recombinant techniques), microbiology, cell biology, biochemistry, and immunology, which are within the skill of the art. These techniques are fully explained in the literature, such as Molecular Cloning: A Laboratory Manual, 2nd Edition (Sambrook et al., 1989) Cold Spring Harbor Press; Oligonucleotide Synthesis (MJ Gait, ed., 1984); Methods in Molecular Biology, Humana Press; Cell Biology: A Laboratory Notebook (JE Cellis, ed., 1998) Academic Press; Animal Cell Culture (RI Freshney, ed., 1987); Introduction to Cell and Tissue Culture (JP Mather and P.E. Roberts, 1998) Plenum Press; Cell and Tissue Culture: Laboratory Procedures (A. Doyle, JB Griffiths and DG Newell, eds., 1993-1998) J. Wiley & Sons; Methods in Enzymology (Academic Press, Inc.); Gene Transfer Vectors for Mammalian Cells (JM Miller and M.P. Calos, eds., 1987); Current Protocols in Molecular Biology (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) (Cell Biology, 2nd Edition) Protocols in Molecular Biology(FMAusubel et al., eds., 1987); PCR: The Polymerase Chain Reaction (Mullis et al., eds., 1994); Sambrook and Russell, Molecular Cloning: A Laboratory Manual, 3rd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (2001); Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, NY (2002); Harlow and Lane, Using Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY (1998); Coligan et al., Short Protocols in Protein Science, John Wiley & Sons, NY (2003); Short Protocols in Molecular Biology (Wiley & Sons, 1999).

[0039] Enzymatic reactions and purification techniques are performed according to manufacturer's specifications, as commonly accomplished in the art or as described herein. Nomenclature utilized in connection with, and the laboratory procedures and techniques of, analytical chemistry, biochemistry, immunology, molecular biology, synthetic organic chemistry, and medicinal and pharmacological chemistry described herein are those well known and commonly used in the art. Standard techniques are used for chemical syntheses and chemical analyses.

[0040] Throughout the present specification and examples, the word "comprises" or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated integer or group of integers but not the exclusion of any other integer or group of integers.

[0041] It should be understood that wherever the language "comprising" is used herein to describe an embodiment, other similar embodiments described as "consisting of" and / or "consisting essentially of" are also provided.

[0042] The term "including" is used to mean "including but not limited to." "Including" and "including but not limited to" are used interchangeably.

[0043] Any examples following the term "such as" or "for example" are not intended to be exhaustive or limiting.

[0044] Unless otherwise required by context, singular terms shall include pluralities and plural terms shall include the singular.

[0045] The articles "a / an" and "the" are used herein to refer to one or more than one (i.e., at least one) of the grammatical object of the article. For example, "element" refers to one element or more than one element. Reference herein to "about" a value or parameter includes (and describes) embodiments involving the value or parameter itself. For example, a description referring to "about X" includes a description of "X." Numerical ranges include the numbers defining the range.

[0046] Although the numerical ranges and parameters setting forth the broad scope of the present invention are approximate, the numerical values set forth in the specific examples are reported as accurately as possible. However, any numerical value inherently contains certain errors necessarily caused by the standard deviation found in its respective test measurements. However, all ranges disclosed herein are understood to encompass any and all subranges contained therein. For example, a range of "1 to 10" should be considered to include any and all subranges (and including the endpoints) between a minimum of 1 and a maximum of 10; that is, all subranges start with a minimum of 1 or greater (e.g., 1 to 6.1) and end with a maximum of 10 or less (e.g., 5.5 to 10).

[0047] Exemplary methods and materials are described herein, but methods and materials similar or equivalent to those described herein can also be used in the practice or testing of the present application. The materials, methods, and examples are illustrative and not intended to be limiting.

[0048] definition

[0049] Unless otherwise indicated, the following terms shall be understood to have the following meanings:

[0050] As used herein, the terms "wild-type virus," "wild-type genome," "wild-type protein," or "wild-type nucleic acid" refer to a sequence of amino acids or nucleic acids that naturally occurs within a population (e.g., a particular viral species, etc.).

[0051] The terms "chimeric" or "engineered" or "modified" (e.g., chimeric vaccinia virus, engineered polypeptide, modified polypeptide, engineered nucleic acid, modified nucleic acid) or grammatical variations thereof are used interchangeably herein to refer to a non-natural sequence that has been manipulated to have one or more changes relative to the native sequence.

[0052] As used herein, "synthetic virus" refers to a virus originally derived from synthetic DNA (e.g., chemically synthesized DNA, PCR amplified DNA, engineered DNA, polynucleotides comprising nucleotide analogs, etc., or a combination thereof) and includes its progeny, and due to natural, accidental or intentional mutations, the progeny may not necessarily be exactly the same as the original parent synthetic virus (in terms of morphology or genomic DNA complementary sequence). In some embodiments, a synthetic virus refers to a virus in which substantially all viral genomes are originally derived from synthetic DNA (e.g., chemically synthesized DNA, PCR amplified DNA, engineered DNA, polynucleotides comprising nucleotide analogs, etc., or a combination thereof). In a preferred embodiment, the synthetic virus is derived from chemically synthesized DNA.

[0053] As outlined elsewhere herein, certain positions of the viral genome may be altered. As used herein, "position" refers to a position in the genomic sequence. Corresponding positions are generally determined by alignment with other parental sequences.

[0054] As used herein, the term "residue" in the context of polypeptides refers to an amino acid unit in a linear polypeptide chain. This is what remains of each amino acid (i.e., -NH-CHR-C-) after removing water from the α-amino acid (i.e., NH2-CHR-COOH) to form a polypeptide.

[0055] As known in the art, "polynucleotide" or "nucleic acid" used interchangeably herein refer to nucleotide chains of any length, and include DNA and RNA. Nucleotides can be deoxyribonucleotides, ribonucleotides, modified nucleotides or bases and / or their analogs, or any matrix that can be incorporated into the chain by DNA or RNA polymerase. Polynucleotides can contain modified nucleotides, such as methylated nucleotides and their analogs. If present, modifications to the nucleotide structure can be imparted before or after chain assembly. The nucleotide sequence can be interrupted by non-nucleotide components. After polymerization, the polynucleotide can be further modified, such as by conjugating with a labeling component. Other types of modifications include, for example, "caps," replacement of one or more naturally occurring nucleotides with analogs; internucleotide modifications, such as, for example, those with uncharged bonds (e.g., methylphosphonates, phosphotriesters, phosphoramidates, carbamates, etc.) and those with charged bonds (e.g., phosphorothioates, phosphorodithioates, etc.); those containing pendant moieties, such as, for example, proteins (e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.); those with intercalators (e.g., acridine, psoralen, etc.); those containing chelators (e.g., metals, radioactive metals, boron, oxidized metals, etc.); those containing alkylating agents; those with modified bonds (e.g., α-anomeric nucleic acids, etc.); and unmodified forms of polynucleotides. In addition, any hydroxyl group typically present in a sugar can be replaced, for example, by a phosphonate group, a phosphate group, protected by standard protecting groups, or activated to prepare additional linkages to additional nucleotides, or can be conjugated to a solid support. 5' and 3' terminal OH can be phosphorylated or partially replaced with amine or organic blocking groups of 1-20 carbon atoms. Other hydroxyls can also be derived into standard protecting groups. Polynucleotides can also contain analog forms of ribose or deoxyribose commonly known in the art, including for example 2'-O-methyl-, 2'-O-allyl, 2'-fluoro- or 2'-azido-ribose, carbocyclic sugar analogs, α- or β-anomeric sugars, epimeric sugars (such as arabinose, xylose or lyxose, pyranose, furanose, sedoheptulose), acyclic analogs and base-free nucleoside analogs such as methyl riboside. One or more phosphodiester bonds can be replaced with alternative linking groups. These alternative linking groups include, but are not limited to, embodiments in which the phosphate is replaced by P(O)S ("thio"), P(S)S ("disulfide"), (O)NR2 ("amide"), P(O)R, P(O)OR', CO, or CH2 ("methylal"), wherein each R or R' is independently H-substituted or unsubstituted alkyl (1-20C) (optionally containing an ether (-O-) linkage), aryl, alkenyl, cycloalkyl, cycloalkenyl, or arylaldehyde. Not all bonds in a polynucleotide are identical. The foregoing description applies to all polynucleotides mentioned herein, including RNA and DNA.

[0056] The terms "polypeptide," "oligopeptide," "peptide," and "protein" are used interchangeably herein to refer to amino acid chains of any length. The chain may be linear or branched, may contain modified amino acids, and / or may be interrupted by non-amino acids. The term also includes amino acid chains that have been modified naturally or by intervention; for example, disulfide bond formation, glycosylation, lipidation, acetylation, phosphorylation, or any other manipulation or modification, such as conjugation with a labeling component. Also included within the definition are, for example, polypeptides containing one or more analogs of amino acids (including, for example, non-natural amino acids, etc.), as well as other modifications known in the art. It should be understood that a polypeptide may exist as a single chain or associated chains.

[0057] "Homologous" in all its grammatical forms and spelling variations refers to the relationship between two proteins (including proteins from superfamilies in the same species of organism, as well as homologous proteins from different species of organism) that have a "common evolutionary origin". These proteins (and their encoding nucleic acids) have sequence homology, as reflected by their sequence similarity (whether by percent identity or by the presence of specific residues or motifs and conserved positions). "Homologous" can also refer to nucleic acids that are native to viruses.

[0058] However, in common usage and in this application, the term "homologous" when modified with an adverb such as "highly" may refer to sequence similarity, and may or may not involve a common evolutionary origin.

[0059] "Heterologous" in all its grammatical forms and spelling variations can refer to a nucleic acid that is not native to a virus. It means originating from a different species or a different strain than the nucleic acid of the organism to which the nucleic acid is described as heterologous. In a non-limiting example, the viral genome of scVACV comprises a heterologous terminal hairpin loop. The heterologous terminal hairpin loop can be derived from a different viral species or from a different VACV strain.

[0060] The term "sequence similarity" in all its grammatical forms refers to the degree of identity or correspondence between nucleic acid or amino acid sequences, which may or may not share a common evolutionary origin.

[0061] " Percentage (%) of sequence identity " or " % sequence identity " relative to a reference polypeptide (or nucleotide) sequence is defined as the percentage of amino acid residues (or nucleic acids) in the candidate sequence that are identical with the amino acid residues (or nucleic acids) in the reference polypeptide (or nucleotide) sequence, after aligning the sequences and introducing gaps (if necessary) to achieve maximum percentage of sequence identity, and without considering any conservative substitutions as part of sequence identity. Comparison for the purpose of determining percentage of amino acid sequence identity can be achieved in various ways within the skill of the art, for example, using publicly available computer software such as BLAST, BLAST-2, ALIGN or Megalign (DNASTAR) software. Those skilled in the art can determine appropriate parameters for aligning sequences, including any algorithm required for achieving maximum alignment over the full length of the sequences being compared.

[0062] As used herein, "host cell" includes a single cell or cell culture that can be or has been a recipient of a virus of the invention. Host cells include the progeny of a single host cell, and due to natural, accidental, or deliberate mutation, the progeny may not necessarily be completely identical to the original parent cell (in morphology or in terms of genomic DNA complement sequence). Host cells include cells transfected and / or transformed in vivo with a poxvirus of the invention.

[0063] As used herein, "vector" refers to a construct capable of delivering and preferably expressing one or more target genes or sequences in a host cell. Examples of vectors include, but are not limited to, viral vectors, naked DNA or RNA expression vectors, plasmids, cosmids or phage vectors, DNA or RNA expression vectors combined with cationic condensing agents, DNA or RNA expression vectors encapsulated in liposomes, and certain eukaryotic cells, such as producer cells.

[0064] As used herein, an "isolated molecule" (wherein the molecule is, for example, a polypeptide, polynucleotide, or fragment thereof) is a molecule that, with respect to its origin or source of derivation, (1) is not associated with one or more naturally associated components with which it is found in its natural state, (2) is substantially free of one or more other molecules from the same species, (3) is expressed by a cell from a different species, or (4) does not occur in nature. Thus, a molecule that is chemically synthesized or expressed in a cellular system that is different from the cell from which it naturally originates will be "isolated" from its naturally associated components. A molecule can also be rendered substantially free of naturally associ...

Claims

1. A synthetic chimeric vaccinia virus (scVACV) replicated and reactivated from DNA derived from chemically synthesized DNA, wherein the genome of the scVACV is different from the corresponding wild-type genome of a vaccinia virus (VACV) strain selected from the group consisting of: Western Reserve (WR), clone 3, Tian Tian, ​​Tian Tian clone TP5, Tian Tian clone TP3, NYCBH, NYCBH clone Acambis 2000, Wyeth, Copenhagen, Lister, Lister 107, Lister-LO, Lister GL-ONC1, Lister GL-ONC2, Lister GL-ONC3, Lister GL-ONC4, ListerCTC1, Lister IMG2 (Turbo FP635), IHD-W, LC16m18, Lederle, Tashkent clone TKT3, Tashkent clone TKT4, USSR, Evans, Praha, L-IVP, V-VET1 or LIVP 6.1.1, Ikeda, EM-63, Malbran, Duke, 3737, CV-1, Connaught Laboratories, Serro 2, CM-01, NYCBH Dryvax, VACV-IOC, Chorioallantoic Vaccinia Virus Ankara (CVA), Modified Vaccinia Virus Ankara (MVA) and MVA-BN, wherein the difference is that the scVACV is characterized by a left terminal hairpin loop and a right terminal hairpin loop, the left terminal hairpin loop and the right terminal hairpin loop are derived from chemically synthesized DNA, one or both of the terminal hairpin loops of the scVACV are heterologous compared to the corresponding wild-type terminal hairpin loop of the scVACV, the loops are independently derived from vaccinia virus, and wherein one or both of the terminal hairpin loops contain a different tandem repeat region or a different number of tandem repeat regions compared to the corresponding wild-type terminal hairpin loop.

2. The scVACV of claim 1, wherein the scVACV further comprises one or more modifications that eliminate one or more unique restriction sites.

3. The scVACV according to claim 1, wherein the scVACV further comprises one or more modifications to add or repair one or more unique restriction sites.

4. The scVACV of claim 2, wherein the one or more modifications to eliminate one or more unique restriction sites comprises eliminating one or more AarI restriction sites.

5. The scVACV of claim 2, wherein the one or more modifications to eliminate one or more unique restriction sites comprises eliminating one or more BsaI restriction sites.

6. The scVACV of claim 1, wherein one or both of the heterologous terminal hairpin loops are derived from a vaccinia virus strain selected from the group consisting of Western Reserve (WR), clone 3, Tian Tian, ​​Tian Tian clone TP5, Tian Tian clone TP3, NYCBH, NYCBH clone Acambis 2000, Wyeth, Copenhagen, Lister, Lister 107, Lister-LO, Lister GL-ONC1, Lister GL-ONC2, Lister GL-ONC3, Lister GL-ONC4, Lister CTC1, Lister IMG2 (Turbo FP635), IHD-W, LC16m18, Lederle, Tashkent clone TKT3, Tashkent clone TKT4, USSR, Evans, Praha, L-IVP, V-VET1, or LIVP 6.1.1, Ikeda, EM-63, Malbran, Duke, 3737, CV-1, Connaught Laboratories, Serro 2, CM-01, NYCBH Dryvax clone DPP13, NYCBH Dryvax clone DPP15, NYCBH Dryvax clone DPP20, NYCBH Dryvax clone DPP17, NYCBH Dryvax clone DPP21, VACV-IOC, Chorioallantoic vaccinia virus Ankara (CVA), modified vaccinia virus Ankara (MVA), and MVA-BN.

7. scVACV according to claim 6, wherein one or both of the heterologous terminal hairpin loops are derived from the VACV Western Reserve (WR) strain.

8. The scVACV according to claim 1, wherein the wild-type genome of the VACV strain is the genome of the NYCBH clone Acambis 2000.

9. The scVACV of claim 1, wherein the NYCBH Dryvax is selected from the group consisting of NYCBH Dryvax clone DPP13, NYCBH Dryvax clone DPP15, NYCBH Dryvax clone DPP20, NYCBH Dryvax clone DPP17, and NYCBH Dryvax clone DPP21.

10. The scVACV according to claim 1, wherein the wild-type genome of the VACV strain is the genome of V-VET1.

11. The scVACV according to claim 1, wherein the wild-type genome of the VACV strain is the genome of the modified vaccinia virus Ankara (MVA).

12. The scVACV according to claim 1, wherein the wild-type genome of the VACV strain is the genome of MVA-BN.

13. The scVACV according to claim 1, wherein the wild-type genome of the VACV strain is the genome of VACV-IOC.

14. The scVACV of claim 7, wherein one or both of the heterologous terminal hairpin loops are independently selected from the slow form and the fast form of the vaccinia virus terminal hairpin loop.

15. The scVACV of claim 14, wherein the slow form comprises a nucleotide sequence that is at least 85% identical to the nucleotide sequence of SEQ ID NO: 13, and the fast form comprises a nucleotide sequence that is at least 85% identical to the nucleotide sequence of SEQ ID NO:

14.

16. The scVACV of claim 14, wherein the slow form comprises a nucleotide sequence that is at least 90% identical to the sequence of SEQ ID NO: 13, and the fast form comprises a nucleotide sequence that is at least 90% identical to the nucleotide sequence of SEQ ID NO:

14.

17. The scVACV of claim 14, wherein the slow form comprises a nucleotide sequence that is at least 95% identical to the nucleotide sequence of SEQ ID NO: 13, and the fast form comprises a nucleotide sequence that is at least 95% identical to the nucleotide sequence of SEQ ID NO:

14.

18. The scVACV of claim 14, wherein the slow form consists of the nucleotide sequence of SEQ ID NO: 13, and the fast form consists of the nucleotide sequence of SEQ ID NO:

14.

19. The scVACV according to any one of claims 1 to 18, wherein the scVACV is replicated and reactivated from DNA derived from overlapping chemically synthesized DNA fragments and DNA derived from chemically synthesized DNA corresponding to the left terminal hairpin loop and the right terminal hairpin loop, wherein the overlapping chemically synthesized DNA fragments are different from the corresponding wild-type genome of vaccinia virus.

20. The scVACV of claim 19, wherein the overlapping chemically synthesized DNA fragments comprise 2-14 overlapping fragments.

21. The scVACV of claim 20, wherein the overlapping chemically synthesized DNA fragments comprise 6-12 overlapping fragments.

22. The scVACV of claim 21, wherein the overlapping chemically synthesized DNA fragments comprise 9 overlapping fragments.

23. The scVACV of claim 1, wherein the scVACV is reactivated using Leporpoxvirus-catalyzed recombination and reactivation.

24. The scVACV according to claim 23, wherein the rabbit poxvirus is selected from the group consisting of Sheppard's fibroma virus (SFV), rabbit fibroma virus, rabbit fibroma virus, squirrel fibroma virus and myxoma virus.

25. A method for preparing a synthetic chimeric vaccinia virus (scVACV), wherein The following steps are involved: (i) chemically synthesizing overlapping DNA fragments corresponding to a wild-type vaccinia virus selected from the group consisting of Western Reserve (WR), clone 3, Tian Tian, ​​Tian Tian clone TP5, Tian Tian clone TP3, NYCBH, NYCBH clone Acambis 2000, Wyeth, Copenhagen, Lister, Lister 107, Lister-LO, Lister GL-ONC1, Lister GL-ONC2, Lister GL-ONC3, Lister GL-ONC4, Lister CTC1, Lister IMG2 (Turbo FP635), IHD-W, LC16m18, Lederle, Tashkent clone TKT3, Tashkent clone TKT4, USSR, Evans, Praha, L-IVP, V-VET1, or LIVP 6.1.1, Ikeda, EM-63, Malbran, Duke, 3737, CV-1, Connaught Laboratories, Serro 2, CM-01, NYCBH Dryvax, VACV-IOC, chorioallantoic vaccinia virus Ankara (CVA), modified vaccinia virus Ankara (MVA), and MVA-BN; (ii) chemically synthesizing DNA corresponding to: (a) The left terminal hairpin loop of vaccinia virus and the right terminal hairpin loop of vaccinia virus; or (b) a left terminal hairpin loop of a vaccinia virus and a right terminal hairpin loop of a vaccinia virus, wherein one or both of the terminal hairpin loops comprise a different tandem repeat region or a different number of tandem repeat regions compared to the corresponding wild-type terminal hairpin loops, wherein one or both of the terminal hairpin loops in (a) and (b) are heterologous compared to the terminal hairpin loop of the corresponding wild-type vaccinia virus, and ligating the terminal hairpin loop of the chemically synthesized DNA derived from (a) or (b) to the left and right ends of the overlapping DNA fragments derived from the chemically synthesized DNA of step (i); (iii) transfecting the ligated DNA from step (ii) into helper virus infected cells; (iv) culturing the cells to produce a mixture of helper virus and scVACV particles in the cells; and (v) plating the mixture on host cells specific for the scVACV to recover the scVACV.

26. The method of claim 25, wherein the helper virus is selected from the group consisting of: rabbit pox virus, fowl pox virus, and psoralen inactivated helper virus.

27. The method of claim 26, wherein the rabbit poxvirus is selected from the group consisting of Sheppard's fibroma virus (SFV), rabbit fibroma virus, rabbit fibroma virus, squirrel fibroma virus, and myxoma virus.

28. The method of claim 25, wherein the helper virus-infected cells are BGMK cells.

29. The method of any one of claims 25 to 28, wherein the overlapping DNA fragments comprise: (i) a nucleotide sequence that is at least 85% identical to the sequence of SEQ ID NOs: 1-9; (ii) a nucleotide sequence that is at least 90% identical to the sequence of SEQ ID NOs: 1-9; (iii) a nucleotide sequence that is at least 95% identical to the sequence of SEQ ID NOs: 1-9; or (iv) a nucleotide sequence consisting of the sequence of SEQ ID NOs: 1–9.

30. A pharmaceutical composition comprising the scVACV of any one of claims 1 to 24 and a pharmaceutically acceptable carrier.

31. The pharmaceutical composition of claim 30, wherein the scVACV is inactivated.

32. The pharmaceutical composition of claim 31, wherein the inactivation is performed by heat, UV or formalin.

33. Use of a composition comprising the scVACV of any one of claims 1 to 24 or a pharmaceutical composition of any one of claims 30 to 32 in the preparation of a medicament for triggering or enhancing an immune response against one or more of vaccinia virus, smallpox virus and monkeypox virus.

34. Use of a composition comprising the scVACV of any one of claims 1 to 24 or a pharmaceutical composition of any one of claims 30 to 32 in the preparation of a medicament, wherein the medicament is for immunizing a human subject to protect the subject from one or more of smallpox virus infection, vaccinia virus infection, and monkeypox virus infection.

35. Use of a composition comprising the scVACV of any one of claims 1 to 24 or a pharmaceutical composition of any one of claims 30 to 32 in the preparation of a medicament, wherein the medicament is used to treat one or more of smallpox virus infection, vaccinia virus infection, and monkeypox virus infection.

36. The use according to any one of claims 33 to 35, wherein the composition comprising the scVACV or the pharmaceutical composition is formulated for skin scarification, intramuscular or intravenous administration.

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