Production Application of Lactic Acid Bacteria Fermentation Technology in the Fine Processing of Chinese Medicinal Material Scutellaria baicalensis Georgi

Abstract

The present invention discloses the production application of lactic acid bacteria fermentation technology in the processing of Chinese medicinal material polygonatum sibiricum. It has changed the traditional processing technology of Chinese medicinal materials. After the pretreatment of raw polygonatum sibiricum, water is added, followed by high-temperature sterilization and cooling; then Lactobacillus plantarum HM6008 is inoculated for constant-temperature fermentation; and then it is dried to form a polygonatum sibiricum Chinese medicinal material product. The said Chinese medicinal material product has a suitable taste, rich nutritional value, and the characteristics of effectively increasing the content of sapogenin, reducing the content of 5-HMF, and increasing the content of active ingredients. The application of this lactic acid bacteria technology in the processing of Chinese medicinal materials can form new application methods and effects for products such as lactic acid bacteria beverages, medicinal diet foods, processing and preparation of Chinese medicinal materials, and medicinal and edible homologous foods such as lactic acid bacteria candies.

Description

Technical Field

[0001] The present invention relates to the field of microbial fermentation, and particularly to a preparation method of a Chinese medicinal material product using polygonatum as a raw material. Background Art

[0002] In the use of polygonatum, the steaming process is mostly adopted, and effective components such as total sapogenins are extracted through steaming. However, 5-hydroxymethylfurfural (5-HMF) will also be generated at the same time.

[0003] The saponins in polygonatum, mainly dioscin, are one of the characteristic and main active components of polygonatum. Total saponins of polygonatum kingianum can effectively reconcile and relieve type 2 diabetes and have an obvious hypoglycemic effect.

[0004] 5-Hydroxymethylfurfural (5-HMF) is a light yellowish-brown solid with a pleasant smell and high chemical reactivity. There are controversies about its safety risks and health care effects. Most research reports believe that 5-HMF is an endogenous harmful component generated in food processing.

[0005] The main result of this study is to obtain a strain of Lactobacillus plantarum, and through the process of lactic acid bacteria fermentation, the total sapogenin is retained to the greatest extent and 5-HMF is generated at the lowest limit. Summary of the Invention

[0006] The purpose of the present invention is to provide a Chinese medicinal material product using polygonatum as a raw material, which has a suitable taste, improved flavor, and at the same time has improved nutritional value and is easy for people to accept.

[0007] To achieve the above purpose, the present invention provides a preparation method of a Chinese medicinal material product using polygonatum as a raw material, which is characterized in that: pretreatment of raw polygonatum: cut raw polygonatum into slices, wash the surface mud with clean water, and set aside;

[0008] Pretreatment before fermentation: add water to polygonatum and sterilize at high temperature, then cool;

[0009] Fermentation: inoculate Lactobacillus plantarum HM6008 and ferment at a constant temperature;

[0010] Filter and collect the supernatant to form a liquid product, or directly dry to form a polygonatum Chinese medicinal material product.

[0011] Further, in the pretreatment step before fermentation, the weight ratio of polygonatum to water is (1-20):100.

[0012] Further, the sterilization condition in the pretreatment before fermentation is sterilization at 100°C - 121°C for 20 - 40 minutes.

[0013] Further, the sterilization condition in the pretreatment before fermentation is sterilization at 100°C - 121°C for 30 minutes.

[0014] Further, during the pre-fermentation treatment, it is cooled to 37 ± 3°C.

[0015] Further, the Lactobacillus plantarum is Lactobacillus plantarum fermentation broth, bacterial powder or strain.

[0016] Further, the inoculation amount in the fermentation step is such that the initial viable cell count reaches 1×10 6 CFU / mL to 1×10 8 CFU / mL after inoculation.

[0017] Further, the constant temperature fermentation time in the fermentation step is 18 - 24 h.

[0018] The present invention also provides a traditional Chinese medicine product prepared from polygonatum sibiricum as the raw material.

[0019] The present invention uses Lactobacillus plantarum HM6008 for fermentation, which has the characteristic of enhancing the taste; other active ingredients are also brought during the fermentation process, increasing the nutritional value, effectively increasing the content of sapogenin, and reducing the content of 5-HMF (5-hydroxymethylfurfural).

[0020] The traditional Chinese medicine product of the present invention can be a lactic acid bacteria beverage, a medicinal diet food, a new process traditional Chinese medicine, a lactic acid bacteria candy, etc. Detailed Description of the Embodiment

[0021] The embodiments of the present invention are described in detail below. The examples of the embodiments are intended to explain the present invention and should not be construed as limiting the present invention. For those without specific technical or conditions noted in the examples, the techniques or conditions described in the literature in the field or according to the product specifications are followed. Those reagents or instruments without the manufacturer noted are all conventional products that can be obtained through commercial purchase.

[0022] The strains used in the following examples are as follows:

[0023] Strain name: Lactobacillus plantarum HM6008,

[0024] Date of deposit: August 10, 2015,

[0025] Depositary institution: Depositary institution: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China, China General Microbiological Culture Collection Center (CGMCC),

[0026] Deposit number: CGMCC No. 11212,

[0027] This strain has been disclosed in CN105238713A on January 13, 2016.

[0028] Strain name: Lactobacillus plantarum HM6068,

[0029] Date of deposit: August 10, 2015,

[0030] Depositary institution: China General Microbiological Culture Collection Center (CGTCC), No. 13, Beiyitiao, Zhongguancun, Haidian District, Beijing, China

[0031] Accession number: CGMCC No. 11214,

[0032] This strain was publicly disclosed in CN106399208A on February 15, 2017.

[0033] Strain name: Lactobacillus plantarum HM60S1,

[0034] Date of deposit: August 10, 2015,

[0035] Depositary institution: China General Microbiological Culture Collection Center (CGMCC), No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China

[0036] Accession number: CGMCC No. 11217,

[0037] This strain was publicly disclosed in CN105132333A on December 9, 2015.

[0038] Strain name: Lactobacillus plantarum HM6055,

[0039] Date of deposit: August 10, 2015,

[0040] Depositary institution: China General Microbiological Culture Collection Center (CGMCC), No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China

[0041] Accession number: CGMCC No. 11213,

[0042] This strain was publicly disclosed in CN105274023A on January 27, 2016.

[0043] The Lactobacillus bulgaricus powder, Lactobacillus casei, and Lactobacillus paracasei purchased from Chr. Hansen.

[0044] Example 1: Preparation of the polygonatum Chinese herbal medicine product

[0045] Pretreatment of raw polygonatum: Cut raw polygonatum into slices, wash the surface mud with clean water, and set aside;

[0046] Pretreatment before fermentation: 10 g of washed polygonatum sibiricum, add 100 g of water, sterilize at 100℃ - 121℃ for 0.5 h, and cool to 37℃;

[0047] Fermentation: Inoculate Lactobacillus plantarum HM6008 to make the viable cell count reach 1×10 6 CFU / mL (referring to the initial viable cell count of the whole environment after inoculating lactic acid bacteria), and ferment at a constant temperature for 18 - 24 hours;

[0048] Dry to form the polygonatum sibiricum Chinese medicinal material product.

[0049] Example 2: Preparation of polygonatum sibiricum Chinese medicinal material product

[0050] Pretreatment of raw polygonatum sibiricum: Cut raw polygonatum sibiricum into slices, wash the surface mud with clean water, and set aside;

[0051] Pretreatment before fermentation: 1 g of washed polygonatum sibiricum, add 100 g of water, sterilize at 100℃ - 121℃ for 20 minutes, and cool to 37℃;

[0052] Fermentation: Inoculate Lactobacillus plantarum HM6008 to make the viable cell count reach 1×10 7 CFU / mL (referring to the initial viable cell count of the whole environment after inoculating lactic acid bacteria), and ferment at a constant temperature for 18 - 24 hours;

[0053] Dry to form the polygonatum sibiricum Chinese medicinal material product.

[0054] Example 3: Preparation of polygonatum sibiricum Chinese medicinal material product

[0055] Pretreatment of raw polygonatum sibiricum: Cut raw polygonatum sibiricum into slices, wash the surface mud with clean water, and set aside;

[0056] Pretreatment before fermentation: 20 g of washed polygonatum sibiricum, add 100 g of water, sterilize at 100℃ - 121℃ for 40 minutes, and cool to 37℃;

[0057] Fermentation: Inoculate Lactobacillus plantarum HM6008 to make the viable cell count reach 1×10 8 CFU / mL (referring to the initial viable cell count of the whole environment after inoculating lactic acid bacteria), and ferment at a constant temperature for 18 - 24 hours;

[0058] Dry to form the polygonatum sibiricum Chinese medicinal material product.

[0059] Comparative example 1: Replace the bacteria with Lactobacillus plantarum HM6068, and the others are the same as in Example 1.

[0060] Comparative example 2: Replace the bacteria with Lactobacillus plantarum HM60S1, and the others are the same as in Example 1.

[0061] Comparative example 3: Replace the bacteria with Lactobacillus plantarum HM6055, and the others are the same as in Example 1.

[0062] Comparative Example 4: The bacteria was changed to Lactobacillus casei, and the others were the same as in Example 1.

[0063] Comparative Example 5: The bacteria was changed to Lactobacillus bulgaricus powder, and the others were the same as in Example 1.

[0064] Comparative Example 6: The bacteria was changed to Lactobacillus paracasei, and the others were the same as in Example 1.

[0065] Comparative Example 7: Treated in the same way as in Example 1, without inoculating lactic acid bacteria, but fermenting under the same environmental temperature.

[0066] Comparative Example 8: Fresh polygonatum sibiricum was washed, sliced, naturally dried in the sun, crushed, sieved through a 60-mesh sieve, and then dried in a forced-air drying oven at 90 °C until constant weight.

[0067] Comparative Example 9: The prepared polygonatum sibiricum Chinese medicinal material product by the nine-steaming and nine-boiling process: put it into a steamer, bring to a boil over high heat, then simmer for one hour over low heat, take it out and dry in the sun. Repeat the above steps nine times to obtain the polygonatum sibiricum Chinese medicinal material product.

[0068] Flavor test: Five flavor evaluators were selected to directly taste the polygonatum sibiricum Chinese medicinal material products with different treatments in Examples 1-3 and Comparative Examples 1-9 (the evaluation criteria are shown in Table 1), and the evaluation contents were recorded as shown in Table 2.

[0069] Table 1 Flavor Evaluation Index Table

[0070] Standard Score (total score: 10 points) With the natural fermentation aroma of lactic acid bacteria, effectively enhancing the taste 8-10 There is no obvious difference in taste between adding lactic acid bacteria fermentation and non-fermentation 6-8 There is an unpleasant flavor brought by fermentation, or the taste becomes worse 3-6 There is an unpleasant flavor brought by fermentation, and at the same time the taste becomes worse 0-3

[0071] Table 2 Effect Verification Result Table of the Dried Polygonatum sibiricum Chinese Medicinal Material Product

[0072]

[0073]

[0074] It can be seen that the highest score of the prepared polygonatum Chinese medicinal material product using Lactobacillus plantarum HM6008 of the present invention and fermenting by the method of the present invention is 9.8. The scores of the polygonatum Chinese medicinal material products prepared by fermentation with other Lactobacillus plantarum in Comparative Examples 1-3 are 2.0-6.3; the score of the polygonatum Chinese medicinal material product prepared by replacing with Lactobacillus casei in Comparative Example 4 is 3.5; the score of the polygonatum Chinese medicinal material product prepared by replacing with Lactobacillus bulgaricus in Comparative Example 5 is 4.0; the score of the polygonatum Chinese medicinal material product prepared by replacing with Lactobacillus paracasei in Comparative Example 6 is 2.5; the score of the polygonatum Chinese medicinal material product obtained by not inoculating lactic acid bacteria but keeping warm in the same environment in Comparative Example 7 is 6.6; the score of the polygonatum Chinese medicinal material product obtained by directly drying and sieving in Comparative Example 8 is 7.1, and the score of the polygonatum Chinese medicinal material product prepared by the traditional nine-steaming and nine-boiling process in Comparative Example 9 is 8, which is also lower than the score of 9.8 of the polygonatum Chinese medicinal material product prepared by fermentation with Lactobacillus plantarum HM6008 of the present invention.

[0075] Nutritional value test: Lactobacillus plantarum HM6008 was compared with each control group in terms of nutritional components (determination of sapogenin content and 5-HMF content), and the results are shown in Table 2. The determination of sapogenin content refers to Sheng Xian, Yang Zhengming, Chen Yijun, etc. Changes in the contents of polysaccharides and saponins during the "nine-steaming and nine-boiling" processing of polygonatum [J]. Journal of Hunan Normal University (Medical Science), 2015, 12(5): 141-144. The determination of 5-HMF content refers to GB / T 18932.18—2003.

[0076] It can be seen that by using Lactobacillus plantarum HM6008 of the present invention and fermenting by using the method of the present invention, the saponin content of the prepared polygonatum Chinese medicinal material product is the highest, which is 0.582%, and the 5-HMF content is the lowest, which is 1340.3 mg·kg-1; for Comparative Examples 1-3, the saponin content of the polygonatum Chinese medicinal material products prepared by fermenting with other Lactobacillus plantarum is 0.254-0.361%, and the 5-HMF content is 3721.54-3422.49 mg·kg-1; for Comparative Example 4, the saponin content of the polygonatum Chinese medicinal material product prepared by replacing it with Lactobacillus casei fermentation is 0.293%, and the 5-HMF content is 3358.97 mg·kg-1; for Comparative Example 5, the saponin content of the polygonatum Chinese medicinal material product prepared by replacing it with Lactobacillus bulgaricus fermentation is 0.281%, and the 5-HMF content is 3448.55 mg·kg-1; for Comparative Example 6, the saponin content of the polygonatum Chinese medicinal material product prepared by replacing it with Lactobacillus paracasei fermentation is 0.279%, and the 5-HMF content is 3125.41 mg·kg-1; for Comparative Example 7, no lactic acid bacteria are inoculated, but the saponin content of the polygonatum Chinese medicinal material product obtained by heat preservation in the same environment is 0.284, and the 5-HMF content is 3248.32 mg·kg-1; for Comparative Example 8, the saponin content of the polygonatum Chinese medicinal material product obtained by directly drying in the sun and sieving is 0.348, and the 5-HMF content is 2229.72 mg·kg-1; for Comparative Example 9, the saponin content of the polygonatum Chinese medicinal material product prepared according to the traditional nine-steaming and nine-boiling process is 0.398, and the 5-HMF content is 3109.67 mg·kg-1.

[0077] Although the embodiments of the present invention have been shown and described above, it can be understood that the above embodiments are exemplary and should not be construed as limiting the present invention. Those of ordinary skill in the art can make changes, modifications, substitutions, and variations to the above embodiments within the scope of the present invention without departing from the principles and purposes of the present invention.

Claims

1. A method for preparing a Chinese medicinal product using Polygonatum sibiricum as raw material, characterized in that: Pretreatment of raw polygonatum: slice the raw polygonatum, wash the surface mud with clean water, and set aside; Pre-fermentation treatment: add water to Polygonatum sibiricum and sterilize at high temperature, then cool; Fermentation: Inoculate Lactobacillus plantarum HM6008 and ferment at a constant temperature; the inoculation amount of the fermentation step is such that the initial viable count reaches 1×10 6 CFU / mL to 1×10 8 CFU / mL; The product is then dried to form the Chinese medicinal product Polygonatum sibiricum.

2. The method for preparing the Chinese medicinal material product using Polygonatum sibiricum as raw material as claimed in claim 1, characterized in that: In the pre-fermentation treatment step, the weight ratio of polygonatum to water is (1-20):

100.

3. The method for preparing the Chinese medicinal material product using Polygonatum sibiricum as raw material as claimed in claim 1, characterized in that: The sterilization condition in the pre-fermentation treatment is 100° C.-121° C. sterilization for 20-40 minutes.

4. The method for preparing the Chinese medicinal material product using Polygonatum sibiricum as raw material as claimed in claim 3, characterized in that: The sterilization condition in the pre-fermentation treatment is sterilization at 100° C.-121° C. for 30 minutes.

5. The preparation method of the Chinese medicinal material product using polygonatum sibiricum as the raw material according to claim 1, characterized in that, During the pre-fermentation treatment, the mixture was cooled to 37±3°C.

6. The preparation method of the traditional Chinese medicine product with polygonatum sibiricum as the raw material according to claim 1, characterized in that, The plant lactobacillus is plant lactobacillus fermentation liquid, bacterial powder or bacterial strain.

7. The method for preparing the Chinese medicinal material product using Polygonatum sibiricum as raw material as claimed in claim 1, characterized in that: The constant temperature fermentation time of the fermentation step is 18-24 hours.

8. A Chinese medicinal product prepared according to any one of claims 1 to 7 using Polygonatum sibiricum as raw material.

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