A method and system for filtration separation of mesenchymal stem cells

Abstract

The present invention discloses a mesenchymal stem cell filtration separation method and system. The operating jaw is installed on the free end of the operating arm through a bearing, and the separation and filtration equipment is clamped and fixed by the operating jaw. Then, the filtration separation equipment is slightly adjusted by rotating the support arm, the steering arm and the operating arm. In addition, the rotatable visual microscope is added to facilitate the filtration separation operation of the mesenchymal stem cells, so that the filtration separation effect of the mesenchymal stem cells is better.

Description

Technical Field

[0001] The present invention relates to the field of biotechnology, and in particular to a method and system for filtering and separating mesenchymal stem cells. Background Art

[0002] Currently, stem cells refer to primitive cells with the potential for self-renewal and multidirectional differentiation. They are the origin cells of the body and can differentiate into one or more cells that constitute human tissues or organs under specific conditions. Stem cells need to be filtered and separated during cultivation.

[0003] The existing separation of stem cells needs to be completed quickly to ensure that the stem cells remain viable in different environments, so operators choose to perform the separation operation on a storage box. However, since the storage box is not convenient for operation, the effect of the filtration separation of mesenchymal stem cells is affected. Summary of the invention

[0004] The purpose of the present invention is to provide a method and system for filtering and separating mesenchymal stem cells, aiming to solve the technical problem in the prior art that operators choose to perform separation operations on storage boxes, which is inconvenient to operate on storage boxes and affects the effect of filtering and separating mesenchymal stem cells.

[0005] To achieve the above-mentioned purpose, the present invention adopts a mesenchymal stem cell filtration separation system, which includes a box and a supporting device; the supporting device includes a supporting frame, a placing table, a visual microscope, an adjustment component and a stabilizing component, the supporting frame is fixedly connected to the box and is located on one side of the box, the placing table is fixedly connected to the supporting frame and is located on a side of the supporting frame away from the box, the visual microscope is rotatably connected to the supporting frame and is located on a side of the supporting frame close to the placing table, the adjustment component is rotatably connected to the supporting frame and fixedly connected to the visual microscope; the stabilizing component includes a supporting arm, a steering arm, an operating arm and an operating clamping claw, the supporting arm is rotatably connected to the supporting frame and is located on a side of the supporting frame away from the visual microscope, the steering arm is rotatably connected to the supporting arm and is located on a side of the supporting arm away from the supporting frame, the operating arm is rotatably connected to the steering arm and is located on a side of the steering arm away from the supporting arm, and the operating clamping claw is rotatably connected to the operating arm and is located on a side of the operating arm away from the steering arm.

[0006] Wherein, the adjustment component includes a storage seat and a steering shaft, the storage seat is fixedly connected to the visual microscope and is located on a side of the visual microscope close to the placement table, the steering shaft is fixedly connected to the storage seat and is rotatably connected to the support frame, and is located on a side of the support frame close to the storage seat.

[0007] Wherein, the stabilizing component further comprises an adjusting dial, which is fixedly connected to the supporting frame and rotatably connected to the supporting arm, and is located on a side of the supporting frame close to the supporting arm.

[0008] Wherein, the stabilizing assembly further comprises a first steering bearing, which is fixedly connected to the support arm and rotatably connected to the steering arm, and is located on a side of the support arm close to the steering arm.

[0009] Wherein, the stabilizing assembly further comprises a second steering bearing, which is fixedly connected to the steering arm and rotatably connected to the operating arm, and is located on a side of the steering arm close to the operating arm.

[0010] Wherein, the stabilizing assembly further comprises a third steering bearing, which is fixedly connected to the operating arm and rotationally connected to the operating clamping claw, and is located on a side of the operating arm close to the operating clamping claw.

[0011] The present invention also includes a method for filtering and separating mesenchymal stem cells, comprising the following steps:

[0012] Take out the stem cell placement tray from the box and place it on the placement table;

[0013] Turn the visual microscope close to the placement plate and install the separation tool on the operating jaws;

[0014] Rotate the operating clamp to drive the operating arm, the steering arm and the support arm to rotate;

[0015] Under the support of the operating clamps, stem cells are filtered and separated.

[0016] The present invention provides a mesenchymal stem cell filtration separation method and system, wherein the operating jaw is installed on the free end of the operating arm through a bearing, and the separation and filtration equipment is clamped and fixed by the operating jaw. Then, the filtration separation equipment is slightly adjusted by rotating the support arm, the steering arm and the operating arm. In addition, the rotatable visual microscope is added to facilitate the filtration separation operation of the mesenchymal stem cells, thereby achieving a better filtration separation effect of the mesenchymal stem cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a structural schematic diagram of the supporting device of the present invention.

[0019] Figure 2 It is a schematic diagram of the structure of the stabilizing assembly of the present invention.

[0020] Figure 3 It is a schematic diagram of the connection structure between the placement tray and the box body of the present invention.

[0021] Figure 4 The present invention is a flow chart of the method for separating mesenchymal stem cells by filtration.

[0022] In the figure: 1-box, 2-support device, 3-placing plate, 21-support frame, 22-placing table, 23-visual microscope, 24-adjusting component, 25-stabilizing component, 26-storage rack, 27-sealing cover, 100-mesenchymal stem cell filtration separation system, 241-storage seat, 242-steering shaft, 251-support arm, 252-steering arm, 253-operating arm, 254-operating clamp, 255-adjusting turntable, 256-first steering bearing, 257-second steering bearing, 258-third steering bearing. DETAILED DESCRIPTION

[0023] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, and should not be construed as limiting the present invention.

[0024] In the description of the present invention, it should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, in the description of the present invention, "multiple" means two or more, unless otherwise clearly and specifically defined.

[0025] See also Figures 1 to 3The present invention provides a mesenchymal stem cell filtration separation system 100, comprising a housing 1 and a support device 2; the support device 2 comprises a support frame 21, a placement table 22, a visual microscope 23, an adjustment component 24 and a stabilization component 25, the support frame 21 is fixedly connected to the housing 1 and is located on one side of the housing 1, the placement table 22 is fixedly connected to the support frame 21 and is located on a side of the support frame 21 away from the housing 1, the visual microscope 23 is rotatably connected to the support frame 21 and is located on a side of the support frame 21 close to the placement table 22, the adjustment component 24 is rotatably connected to the support frame 21 and is fixedly connected to the visual microscope 23 Fixed connection; the stabilizing component 25 includes a support arm 251, a steering arm 252, an operating arm 253 and an operating clamp 254, the support arm 251 is rotatably connected to the support frame 21 and is located on a side of the support frame 21 away from the visual microscope 23, the steering arm 252 is rotatably connected to the support arm 251 and is located on a side of the support arm 251 away from the support frame 21, the operating arm 253 is rotatably connected to the steering arm 252 and is located on a side of the steering arm 252 away from the support arm 251, and the operating clamp 254 is rotatably connected to the operating arm 253 and is located on a side of the operating arm 253 away from the steering arm 252.

[0026] In this embodiment, the box body 1 is a rectangular body with a hollow interior, and has an opening on the front side of the box body 1 for placing the placement tray 3. The placement tray 3 is placed on the storage rack 26, and the number of the storage racks 26 is two, so that multiple placement trays 3 can be placed; the top of the box body 1 is threadedly installed with the support frame 21, and the support frame 21 supports the placement table 22, and the placement table 22 is used to install the placement tray 3, so that the placement tray 3 can be quickly taken out and placed on the placement table 22; the visual microscope 23 is installed on the left side of the support frame 21 through the adjustment component 24, so that the visual microscope 23 can be rotated to magnify the mesenchymal stem cells in the placement tray 3, so as to facilitate filtering and separation; the support frame 21 is installed on the right side of the support frame 21 through the bearing The support arm 251 is axially rotated, and the steering arm 252 is installed at the free end of the support arm 251 through a bearing. The steering arm 252 can tilt and rotate around the support arm 251. The operating arm 253 is rotatably connected to the steering arm 252 through a rotating shaft and can rotate around the steering arm 252. The operating clamp 254 is installed at the free end of the operating arm 253 through a bearing. The separation and filtration equipment is clamped and fixed by the operating clamp 254, and then the filtration separation equipment is slightly adjusted by rotating the support arm 251, the steering arm 252 and the operating arm 253. In addition, the rotatable visual microscope 23 facilitates the filtration separation operation of the mesenchymal stem cells, so that the filtration separation effect of the mesenchymal stem cells is better.

[0027] For further information, see Figure 1 The adjustment component 24 includes a storage seat 241 and a steering shaft 242. The storage seat 241 is fixedly connected to the visual microscope 23 and is located on a side of the visual microscope 23 close to the placement table 22. The steering shaft 242 is fixedly connected to the storage seat 241 and is rotatably connected to the support frame 21, and is located on a side of the support frame 21 close to the storage seat 241.

[0028] In this embodiment, the storage seat 241 is sleeved on the outer side of the visual microscope 23 and is rotatably connected to the support frame 21 through the steering shaft 242, so that the steering of the visual microscope 23 can be adjusted, which is more convenient to use.

[0029] For further information, see Figure 2 The stabilizing component 25 further includes an adjusting dial 255 , which is fixedly connected to the supporting frame 21 , and rotatably connected to the supporting arm 251 , and is located on a side of the supporting frame 21 close to the supporting arm 251 .

[0030] In this embodiment, the bottom of the adjusting dial 255 is threadedly mounted on the right side of the support frame 21, and the top is rotatably connected to the support arm 251 through a bearing, thereby driving the support arm 251 to perform axial rotation.

[0031] For further information, see Figure 2 The stabilizing assembly 25 further includes a first steering bearing 256 , which is fixedly connected to the support arm 251 and rotatably connected to the steering arm 252 , and is located on a side of the support arm 251 close to the steering arm 252 .

[0032] In this embodiment, the outer ring of the first steering bearing 256 is threadedly fixed to the support arm 251, and the inner ring of the first steering bearing 256 is threadedly installed to the steering arm 252, and is driven to rotate by the balls between the outer ring and the inner ring, so that the steering arm 252 rotates around the support arm 251.

[0033] For further information, see Figure 2 The stabilizing assembly 25 further includes a second steering bearing 257 , which is fixedly connected to the steering arm 252 , rotatably connected to the operating arm 253 , and is located on a side of the steering arm 252 close to the operating arm 253 .

[0034] In this embodiment, the outer ring of the second steering bearing 257 is threadedly fixed to the steering arm 252, and the inner ring of the second steering bearing 257 is threadedly installed to the operating arm 253, and is driven to rotate by the balls between the outer ring and the inner ring, so that the operating arm 253 rotates around the steering arm 252.

[0035] For further information, see Figure 2 The stabilizing assembly 25 further includes a third steering bearing 258 , which is fixedly connected to the operating arm 253 , and rotatably connected to the operating clamp 254 , and is located on a side of the operating arm 253 close to the operating clamp 254 .

[0036] In this embodiment, the outer ring of the third steering bearing 258 is threadedly mounted on the free end of the operating arm 253 and is threadedly mounted with the operating jaw 254 via an inner ring, so that the operating jaw 254 can rotate around the operating arm 253 .

[0037] For further information, see Figure 3 The supporting device 2 further includes a storage rack 26 , which is fixedly connected to the box body 1 and is located inside the box body 1 .

[0038] In this embodiment, the storage rack 26 is threadedly mounted on the side wall inside the box body 1 and supports the placement tray 3, so that the placement tray 3 can be stably placed in the box body 1 for storage.

[0039] For further information, see Figure 1 The supporting device 2 further includes a sealing cover plate 27 , which is rotatably connected to the box body 1 and is located on a side of the box body 1 close to the storage rack 26 .

[0040] In this embodiment, the sealing cover plate 27 is rotatably connected to the box body 1 via a rotating shaft and covers the open side of the box body 1, so that the storage space inside the box body 1 can be sealed when the disk 3 is stored.

[0041] See also Figure 4 , a method for separating mesenchymal stem cells by filtration, comprising the following steps:

[0042] S901: Take out the stem cell placement tray 3 from the box 1 and place it on the placement table 22;

[0043] S902: Rotate the visual microscope 23 to be close to the placement plate 3, and install the separation tool on the operating clamp 254;

[0044] S903: Rotate the operating jaw 254 to drive the operating arm 253, the steering arm 252 and the support arm 251 to rotate;

[0045] S904: With the support of the operating clamp 254, the stem cells are filtered and separated.

[0046] In this embodiment, the operating jaw 254 is installed on the free end of the operating arm 253 through a bearing. The separation and filtration equipment is clamped and fixed by the operating jaw 254, and then the filtration separation equipment is slightly adjusted by rotating the support arm 251, the steering arm 252 and the operating arm 253. In addition, the rotatable visual microscope 23 facilitates the filtration separation operation of the mesenchymal stem cells, thereby improving the filtration separation effect of the mesenchymal stem cells.

[0047] What is disclosed above is only a preferred embodiment of the present invention, and it certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope of the invention.

Claims

1. A mesenchymal stem cell filtration separation system, characterized in that: It includes a box body and a supporting device; The support device includes a support frame, a placement table, a visual microscope, an adjustment component and a stabilization component. The support frame is fixedly connected to the box and is located on one side of the box. The placement table is fixedly connected to the support frame and is located on a side of the support frame away from the box. The visual microscope is rotatably connected to the support frame and is located on a side of the support frame close to the placement table. The adjustment component is rotatably connected to the support frame and is fixedly connected to the visual microscope. The stabilizing assembly comprises a supporting arm, a steering arm, an operating arm and an operating clamping claw, wherein the supporting arm is rotatably connected to the supporting frame and is located at a side of the supporting frame away from the visual microscope, the steering arm is rotatably connected to the supporting arm and is located at a side of the supporting arm away from the supporting frame, the operating arm is rotatably connected to the steering arm and is located at a side of the steering arm away from the supporting arm, and the operating clamping claw is rotatably connected to the operating arm and is located at a side of the operating arm away from the steering arm; The supporting device also includes a storage rack and a sealing cover plate. The storage rack is fixedly connected to the box body and is located in the box body. The sealing cover plate is rotatably connected to the box body and is located on one side of the box body close to the storage rack.

2. The mesenchymal stem cell filtration separation system according to claim 1, characterized in that: The adjustment component includes a storage seat and a steering shaft, the storage seat is fixedly connected to the visual microscope and is located on a side of the visual microscope close to the placement table, the steering shaft is fixedly connected to the storage seat and rotatably connected to the support frame, and is located on a side of the support frame close to the storage seat.

3. The mesenchymal stem cell filtration separation system according to claim 1, characterized in that: The stabilizing assembly further comprises an adjusting dial, which is fixedly connected to the supporting frame and rotatably connected to the supporting arm, and is located on a side of the supporting frame close to the supporting arm.

4. The mesenchymal stem cell filtration separation system according to claim 1, characterized in that: The stabilizing assembly further comprises a first steering bearing, which is fixedly connected to the support arm and rotatably connected to the steering arm, and is located on a side of the support arm close to the steering arm.

5. The mesenchymal stem cell filtration separation system according to claim 1, characterized in that: The stabilizing assembly further comprises a second steering bearing, which is fixedly connected to the steering arm and rotatably connected to the operating arm, and is located on a side of the steering arm close to the operating arm.

6. The mesenchymal stem cell filtration separation system according to claim 1, characterized in that: The stabilizing assembly further comprises a third steering bearing, which is fixedly connected to the operating arm and rotationally connected to the operating clamping claw, and is located on a side of the operating arm close to the operating clamping claw.

7. A method for filtration separation of mesenchymal stem cells, applicable to the mesenchymal stem cell filtration separation system according to any one of claims 1 to 6, characterized in that: The following steps are included: Take out the stem cell placement tray from the box and place it on the placement table; Turn the visual microscope close to the placement plate and install the separation tool on the operating jaws; Rotate the operating clamp to drive the operating arm, the steering arm and the support arm to rotate; Under the support of the operating clamps, stem cells are filtered and separated.

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