Use of a fungicidal composition containing cedrol and plant essential oils and their main components in the control of plant diseases and molds

Abstract

The application discloses a bactericidal composition containing hinokitiol and plant essential oil and main components thereof, and effective components are hinokitiol and oregano essential oil, clove essential oil, thyme essential oil, carvacrol, eugenol and thymol. The mass fraction ratio of the hinokitiol and the oregano essential oil, the clove essential oil, the thyme essential oil, the carvacrol, the eugenol and the thymol is 1:1, 1:10, 1:30 and 1:50. The bactericidal composition can be used for preventing and treating rhynchosporium, sclerotinia sclerotiorum, tomato gray mold, wheat scab, rice blast, cotton wilt and aspergillus flavus. The components of the application are all natural products and harmless to the environment. The application can reduce the influence of pesticides on the environment, reduce the cost of using pesticides, delay or overcome the generation of disease resistance, prolong the service life of the pesticides, and is beneficial to the comprehensive management of diseases.

Description

TECHNICAL FIELD

[0001] The present application relates to a fungicidal composition, which is used for preventing and treating plant diseases caused by Rhizoctonia solani, Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, Magnaporthe oryzae and Aspergillus flavus, and the use of cypressol and essential oil and its main components as effective components. BACKGROUND

[0002] Plant pathogenic fungi are one of the most common and harmful diseases in crop cultivation. According to the estimate of the Food and Agriculture Organization of the United Nations, the annual loss of grain and cotton production due to diseases is more than 10% worldwide. China is a large agricultural and grain-producing country. Wheat and rice are the main grain crops, and rape, tomato, cotton, potato, corn, watermelon, and pepper are the main economic crops. At present, all of them are suffering from diseases to varying degrees, which not only leads to a reduction in crop yield and causes huge economic losses, but also seriously threatens the quality and safety of agricultural products and international trade. Grain reserves are an effective means to avoid food shortages. Cereals are prone to Aspergillus flavus, which poses a great risk to food safety. Among the many measures to prevent and control plant pathogenic fungi, chemical control is still the mainstream method, and it can achieve high control efficiency in the early stage of prevention and control. However, due to long-term unreasonable use, fungi have developed varying degrees of resistance to these chemical fungicides, resulting in the need for increased dosage to achieve ideal fungicidal effect, and further causing more drug residues and stronger side effects. In order to cope with this drug resistance and reduce the use of chemical fungicides, combined use of drugs is particularly important, which not only can reduce the amount of drug used, increase the sensitivity of the pathogen to fungicides, expand the antibacterial spectrum, and reduce environmental pollution, but also more importantly, can slow down the emergence of fungicide resistance.

[0003] Cypressol is a natural monoterpenoid compound extracted from Chamaecyparis formosana, which has antibacterial, antioxidant, and antitumor effects, and is a highly safe plant secondary metabolite. Studies have shown that cypressol can control postharvest diseases of peaches and eggplants and maintain postharvest quality.

[0004] Essential oil (EO) is a general term for volatile oil-like liquid substances with aroma (and / or fragrance) extracted from plant tissues such as roots, stems, leaves, flowers, fruits, seeds, branches, or secretions. The main components are terpenes, phenols, aldehydes, alcohols, and esters, etc. organic matter. In recent decades, research has found that essential oils and single components have strong characteristics of inhibiting or killing bacteria and fungi and other microorganisms.

[0005] The experiment found that the combination of cypress alcohol and plant essential oil and its main components has obvious synergistic effect in preventing and treating rhizoctonia solani, sclerotinia sclerotiorum, tomato botrytis blight, wheat scab, rice blast, cotton fusarium wilt and aspergillus flavus. While improving the prevention and treatment effect, the drug dosage can be reduced, meeting the demand of "zero growth of pesticides". SUMMARY

[0006] The purpose of the present application is to provide a fungicide composition containing cypress alcohol and plant essential oil and its main components for preventing and treating rhizoctonia solani, sclerotinia sclerotiorum, tomato botrytis blight, wheat scab, rice blast, cotton fusarium wilt and aspergillus flavus.

[0007] To achieve the above purpose, the technical solution of the present application is as follows:

[0008] A fungicide composition containing cypress alcohol and plant essential oil and its main components, comprising active component A and active component B, the active component A is cypress alcohol, the active component B is plant essential oil and its main components, and the weight ratio of active component A to active component B is 1:1, 1:10, 1:30 or 1:50.

[0009] The fungicide composition provided by the present application has the following beneficial effects:

[0010] 1) The fungicide composition provided by the present application shows obvious synergistic effect within a certain ratio range, and the prevention and treatment effect of the composition is obviously improved compared with single agent.

[0011] 2) The fungicide composition provided by the present application reduces the drug cost while improving the prevention and treatment effect, and expands the antibacterial spectrum.

[0012] 3) The fungicide composition provided by the present application is a safe and green natural product, harmless to the environment, and meets the requirements of green agriculture and green development.

[0013] 4) The effective components of the fungicide composition provided by the present application have different mechanisms of action, the application of the composition can delay or overcome the development of disease resistance, prolong the service life of the pesticide, and is beneficial to the comprehensive control of diseases. DETAILED DESCRIPTION

[0014] In order to better understand the essence of the present application, the following examples are further described, but the present application is not limited to these examples. In these examples, all percentages are weight percentages unless otherwise specified.

[0015] Example 1: A fungicide composition containing cypress alcohol and plant essential oil and its main components, comprising active component A and active component B (as shown in structural formula 1), the active component A is cypress alcohol, and the active component B is plant essential oil and its main components.

[0016]

[0017] Example 2: Synergistic effect of thujaplicin and active component B on Sclerotinia sclerotiorum in vitro

[0018] The plant pathogenic fungi used in this experiment were the strains preserved in the laboratory at 4℃, and the culture medium used was potato agar glucose medium (PDA for short). The PDA medium formula was: potato (peeled) 200 g, glucose 20 g, agar 15 g, distilled water 1000 mL, natural PH.

[0019] The synergistic effect of fungicide mixture was calculated according to the formula of Wadley (1967) after mixing different pesticides:

[0020]

[0021]

[0022] In the formula, a, b respectively represent the proportion of thujaplicin (A) and plant essential oil and its main component (B) in the mixture, EC 50 (A), EC 50 (B) respectively represent the actual observed EC 50 value, EC 50 (Exp) represents the theoretical EC 50 value of A, B two kinds of pesticides mixed according to a:b, EC 50 (Obs) is the actual observed EC 50 value of A, B two kinds of pesticides mixed according to a:b. SR>1.5 is synergistic; SR<0.5 is antagonistic; SR between 0.5 and 1.5 indicates additive effect.

[0023] The synergistic effect of thujaplicin and active component B on Sclerotinia sclerotiorum in vitro is shown in Table 1.

[0024] Table 1. Synergistic effect of thujaplicin and active component B on Sclerotinia sclerotiorum in vitro

[0025]

[0026] As can be seen from Table 1, thujaplicin and active component B mixed in mass ratio of 1:1, 1:10, 1:30, 1:50 have obvious synergistic effect on Sclerotinia sclerotiorum, especially thujaplicin and clove essential oil in mass ratio of 1:50, SR is 2.13.

[0027] Example 2: Synergistic effect of thujaplicin and active component B on Sclerotinia sclerotiorum in vitro

[0028] The indoor virulence synergistic effect of cypressol and active component B on Sclerotinia sclerotiorum is shown in Table 2.

[0029] Table 2. Indoor virulence synergistic effect of cypressol and active component B on Sclerotinia sclerotiorum

[0030]

[0031]

[0032] From Table 2, it can be seen that cypressol and active component B have synergistic effect on Sclerotinia sclerotiorum when the mass ratio is 1:1, 1:10, 1:30, and 1:50, especially when cypressol is compounded with plant essential oil, which has obvious synergistic effect. When the mass ratio is 1:1, the SR values of cypressol and the three plant essential oils are 41.69, 27.28 and 14.81 respectively.

[0033] Example 3: Indoor activity determination of cypressol and active component B on tomato gray mold

[0034] The indoor virulence synergistic effect of cypressol and active component B on tomato gray mold is shown in Table 3.

[0035] Table 3. Indoor virulence synergistic effect of cypressol and active component B on tomato gray mold

[0036]

[0037]

[0038] From Table 3, it can be seen that cypressol and active component B have no synergistic effect on tomato gray mold when the mass ratio is 1:1, 1:10, 1:30, and 1:50, and most of the compositions are in additive effect. Only when cypressol:clove essential oil is 1:50, it shows synergistic effect, and the SR is 1.65.

[0039] Example 4: Indoor activity determination of cypressol and active component B on wheat scab

[0040] The indoor virulence synergistic effect of cypressol and active component B on wheat scab is shown in Table 4.

[0041] Table 4. Indoor virulence synergistic effect of cypressol and active component B on wheat scab

[0042]

[0043]

[0044] From Table 4, it can be seen that the mixture of cypress alcohol and active component B in a mass ratio of 1:1, 1:10, 1:30 and 1:50 has no synergistic effect on wheat scab, and all the compositions are in additive effect.

[0045] Example 5: Indoor activity determination of mixture of cypress alcohol and active component B on rice blast

[0046] The indoor virulence synergistic effect of mixture of cypress alcohol and active component B on rice blast is shown in Table 5.

[0047] Table 5. Indoor virulence synergistic effect of mixture of cypress alcohol and active component B on rice blast

[0048]

[0049]

[0050] From Table 5, it can be seen that the mixture of cypress alcohol and active component B in a mass ratio of 1:1, 1:10, 1:30 and 1:50 has no synergistic effect on rice blast, and all the compositions are in additive effect.

[0051] Example 6: Indoor activity determination of mixture of cypress alcohol and active component B on cotton fusarium

[0052] The indoor virulence synergistic effect of mixture of cypress alcohol and active component B on cotton fusarium is shown in Table 6.

[0053] Table 6. Indoor virulence synergistic effect of mixture of cypress alcohol and active component B on cotton fusarium

[0054]

[0055]

[0056] From Table 6, it can be seen that the mixture of cypress alcohol and active component B in a mass ratio of 1:1, 1:10, 1:30 and 1:50 has no synergistic effect on cotton fusarium, and all the compositions are in additive effect.

[0057] Example 7: Indoor activity determination of mixture of cypress alcohol and active component B on aspergillus flavus

[0058] The indoor virulence synergistic effect of mixture of cypress alcohol and active component B on aspergillus flavus is shown in Table 7.

[0059] Table 7. Indoor virulence synergistic effect of mixture of cypress alcohol and active component B on aspergillus flavus

[0060]

[0061]

[0062] From Table 7, it can be seen that the mixture of cypress alcohol and active component B has obvious synergistic effect on Aspergillus flavus when the mass ratio is 1:1, 1:10, 1:30 and 1:50, especially when the mass ratio is 1:1, 1:10 and 1:50, the SR is 4.35 at most, and the rest of the compositions are in additive effect.

[0063] In summary, it can be seen that the antibacterial activity of the combination of cypress alcohol and plant essential oil and its main component composition is higher than that of single agent at the same concentration, and the above compositions are particularly prominent in the prevention and control of Sclerotinia sclerotiorum, Magnaporthe grisea and Aspergillus flavus, and show obvious synergistic effect, for example, cypress alcohol: oregano essential oil = 1:1, the SR against Sclerotinia sclerotiorum is as high as 41.69; cypress alcohol: clove essential oil = 1:1, the SR against Magnaporthe grisea is 4.54; cypress alcohol: carvacol = 1:10, the SR against Magnaporthe grisea is 3.45; all the compositions show synergistic effect and additive effect, and have no antagonistic effect. The present application is conducive to prolonging the service life of the pesticide and conducive to the comprehensive management of diseases.

Claims

1. Use of a fungicidal composition comprising cedrol and oregano essential oil for the control of Sclerotinia sclerotiorum in Brassica napus, characterized in that The mass ratio of cypress alcohol and oregano essential oil was 1:1, 1:10 and 1:30, respectively. The mass ratio of cypress alcohol and oregano essential oil was 1:1, 1:10 and

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