A closed nucleic acid detection consumable and detection method

Abstract

The present invention relates to the field of nucleic acid detection technology, and discloses a closed nucleic acid detection consumable, including a connector, a sample tube and a reaction tube. The connector is provided with a sample puncture tube and a reaction puncture tube at a certain angle thereto, the sample puncture tube and the reaction puncture tube are located on the same side of the connector and are respectively located at both ends of the connector, the connector is provided with a flow channel connecting the sample puncture tube and the reaction puncture tube, the connector is provided with a first positioning seat connected to the sample puncture tube and a second positioning seat connected to the reaction puncture tube, the reaction tube is plugged and connected with the second positioning seat to achieve a fully closed connection, prevent sample leakage, avoid infection, and prevent sample contamination, and can achieve quantitative transfer of samples and retention of samples, which is highly practical and suitable for mass production. In addition, a closed nucleic acid detection method is provided, using the above-mentioned consumables to transfer the sample from the sample to the flow channel and then to the reaction tube through two centrifugations.

Description

Technical Field

[0001] The present invention relates to the technical field of nucleic acid detection, and in particular to a closed nucleic acid detection consumable and a detection method. Background Art

[0002] In the field of PCR testing for infectious diseases, sample preservation fluid or nucleic acid releaser is usually pre-injected into the sample tube, and then the collected sample is placed in the sample tube, which is sealed and then transported to the testing site for testing. During testing, the sample tube cap needs to be reopened for sampling, and a quantitative sample solution is dripped into the reaction tube containing the nucleic acid amplification reaction reagent. Since the sample tube cap is open and in contact with the external environment, there is a risk of infection of the tester or contamination of the sample. And the test usually needs to be carried out in a place with the corresponding level of protection, which is not conducive to the rapid diagnosis and screening of infectious diseases.

[0003] Chinese invention patent CN102534011A (published on July 4, 2012) discloses a fully enclosed method and device for rapid fluorescence detection of target nucleic acid amplification products. The method is that after the amplification reaction is completed, the reaction tube is placed in a closed device without opening the cover. The wall of the reaction tube is broken in the closed device, so that the amplification product in the reaction tube reacts with the detection liquid preset in the closed device, and then fluorescence detection is performed to interpret the result. The device includes a PCR tube, a centrifuge tube and a thimble plug. The PCR tube can be placed in the centrifuge tube. The thimble plug has a puncture needle that can pierce the PCR tube, and the centrifuge tube has a sealable tube cover. The patent is that by placing the PCR tube in the centrifuge tube, when the tube cover of the centrifuge tube is closed, the tube cover exerts pressure on the PCR tube, and the thimble plug in the centrifuge tube will pierce the PCR tube, so that the solution in the PCR tube falls into the centrifuge tube, thereby realizing the transfer of the solution in a sealed environment. However, this patent is to achieve the transfer of the amplified nucleic acid solution, and does not disclose how to transfer the sample solution placed in the sample tube to the PCR tube after sampling. In addition, the existing PCR tubes have already used fluorescent PCR solutions, so there is no need to transfer to centrifuge tubes. If the sample solution in the sample tube is transferred to the PCR reaction tube through the structure of this patent, the transfer can be achieved in a closed environment to avoid infection. However, the structure of this patent will cause all the solution in the sample tube to enter the PCR reaction tube, resulting in excessive sample, affecting the nucleic acid test results, and the sample solution cannot be retained and cannot be repeatedly tested. Summary of the invention

[0004] The object of the present invention is to provide a closed nucleic acid detection consumable and a detection method that can quantitatively transfer samples and retain samples.

[0005] In order to achieve the above-mentioned purpose, the present invention provides a closed nucleic acid detection consumable, including a connector, a sample tube and a reaction tube, the connector is provided with a sample puncture tube and a reaction puncture tube at a certain angle thereto, the sample puncture tube and the reaction puncture tube are located on the same side of the connector and are respectively located at two ends of the connector, the connector is provided with a flow channel connecting the sample puncture tube and the reaction puncture tube, the connector is provided with a first positioning seat and a second positioning seat, the sample puncture tube passes through the first positioning seat, the sample tube is plug-connected to the first positioning seat, the reaction puncture tube passes through the second positioning seat, and the reaction tube is plug-connected to the second positioning seat.

[0006] As a preferred embodiment, the reaction puncture tube is connected to one end of the flow channel, and the connection point between the sample puncture tube and the flow channel is at a certain distance from the other end of the flow channel, so that the connection between the sample puncture tube and the flow channel is located between the other end of the flow channel to form a quantitative chamber.

[0007] As a preferred solution, the sample puncture tube is provided with a main pipeline and a connecting pipeline, the main pipeline is connected with the flow channel through the connecting pipeline, and the diameter of the connecting pipeline is smaller than the diameter of the main pipeline.

[0008] As a preferred solution, a leakage groove is provided on the bottom surface of the flow channel, the leakage groove is located at one end where the reaction puncture tube is connected to the connector, and the reaction puncture tube is in communication with the leakage groove.

[0009] As a preferred solution, the sample tube is threadedly connected to the first positioning seat, and the reaction tube is threadedly connected to the second positioning seat.

[0010] As a preferred solution, the sample tube is snap-connected to the first positioning seat, and the reaction tube is snap-connected to the second positioning seat.

[0011] As a preferred solution, the side of the connector facing away from the sample puncture tube and the reaction puncture tube is a transparent plate.

[0012] As a preferred solution, the connector, the sample tube and the reaction tube are all arranged in plurality, and the plurality of connectors are arranged in parallel and sequentially connected into a whole, so that the plurality of sample tubes are arranged in rows on one side of the whole, and the reaction tubes are arranged in rows on the other side of the whole.

[0013] The present invention also provides a closed nucleic acid detection method, comprising the following steps:

[0014] (1) A sample puncture tube and a reaction puncture tube are vertically connected at both ends of the connector, and a flow channel connecting the sample puncture tube and the reaction puncture tube is provided in the connector, wherein the reaction puncture tube is connected to one end of the flow channel, and a certain distance is provided between the connecting point between the sample puncture tube and the flow channel and the other end of the flow channel, so that a quantitative chamber is formed between the connecting point between the sample puncture tube and the flow channel and the other end of the flow channel;

[0015] (2) plugging a sample tube containing a nucleic acid sample onto the connector so that the sample puncture tube is inserted into the sample tube, and plugging a reaction tube containing a nucleic acid amplification reaction reagent onto the connector so that the reaction puncture tube is inserted into the reaction tube, wherein the length of the reaction puncture tube inserted into the reaction tube is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube;

[0016] (3) Tilt or horizontally place the entirety of the connector, the sample tube, and the reaction tube so that the quantitative chamber and the bottom end of the reaction tube are located on the same straight line, and take a certain point on the straight line where the quantitative chamber and the reaction tube are located as the first rotation center, wherein the bottom end of the reaction tube is closer to the first rotation center than the quantitative chamber, and then centrifuge the entirety of the connector, the sample tube, and the reaction tube around the first rotation center so that the liquid in the sample tube is transferred to the quantitative chamber;

[0017] (4) Invert the entirety of the connector, the sample tube, and the reaction tube after being connected, select a point on a straight line that intersects the connector and passes through the bottom end of the reaction tube as a second rotation center, the quantitative chamber is closer to the second rotation center relative to the bottom end of the reaction tube, and the second rotation center, the quantitative chamber, and the bottom end of the reaction tube are not located on the same straight line, and then centrifuge the entirety of the connector, the sample tube, and the reaction tube after being connected around the second rotation center to transfer the liquid in the quantitative chamber to the reaction tube.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] The present invention arranges a sample puncture tube and a reaction puncture tube on the connector. When the sample tube and the reaction tube are plugged into the first positioning seat and the second positioning seat respectively so that the sample tube, the reaction tube and the connector form an integral body, the sample puncture tube pierces the sample tube and extends into the sample tube, and the reaction puncture tube pierces the reaction tube and extends into the reaction tube, so that the sample tube tightened after sampling and the reaction tube containing PCR reaction reagents can be directly connected to the connector without opening the tube cover, thereby realizing a fully closed connection, preventing the sample in the sample tube from leaking out, avoiding infection, and preventing the sample from being contaminated; the sample tube, the reaction tube and the connector are connected. After the connector is connected, the solution in the sample tube can enter the flow channel through the sample puncture tube by inversion, shaking or centrifugation, and then the sample solution in the flow channel can flow into the reaction tube by inversion, shaking or centrifugation. Due to the limited space of the flow channel, the solution in the sample tube cannot all flow into the flow channel, and the quantitative transfer of the sample solution in the sample tube to the reaction tube can be achieved. After the sample is transferred, the connector is located above the sample tube and the reaction tube, and the solution in the sample tube will no longer flow into the reaction tube through the flow channel, and the unreacted sample can be retained. When it is necessary to detect again, the sample tube can be removed and taken out for use. The consumables of the present invention are simple in structure and highly practical, which is conducive to mass production. In addition, the present invention also provides a closed nucleic acid detection method, which is centrifuged twice to rotate the sample from the sample tube to the flow channel of the connector and then transfer it to the reaction tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the structure of the closed nucleic acid detection consumables of Example 1 of the present invention.

[0021] Figure 2 It is an exploded view of the closed nucleic acid detection consumables of Example 1 of the present invention.

[0022] Figure 3 It is a cross-sectional view of the closed nucleic acid detection consumables according to the first embodiment of the present invention.

[0023] Figure 4 It is a schematic structural diagram of a connector according to a first embodiment of the present invention.

[0024] Figure 5 Schematic diagram of the structure of the sample tube according to the first embodiment of the present invention.

[0025] Figure 6 It is a schematic diagram of the structure of the reaction tube of Example 1 of the present invention.

[0026] Figure 7 It is a schematic diagram of the structure of the closed nucleic acid detection consumables of the second embodiment of the present invention.

[0027] Figure 8 It is a schematic diagram of the structure of the closed nucleic acid detection consumables of embodiment 3 of the present invention.

[0028] Fig. 9 Schematic diagram of step (3) of the closed nucleic acid detection method of embodiment 1 of the present invention.

[0029] Fig.10 Schematic diagram of step (4) of the closed nucleic acid detection method of embodiment 1 of the present invention.

[0030] In the figure, 1-connector; 101-flow channel; 102-quantitative chamber; 103-leakage groove; 2-sample tube; 201-snap hole; 3-reaction tube; 301-snap edge; 4-sample puncture tube; 401-main pipeline; 402-connecting pipeline; 5-reaction puncture tube; 6-first positioning seat; 7-second positioning seat; 701-positioning block; 702-snap protrusion; 8-snap block; 9-first rotation center; 10-second rotation center. DETAILED DESCRIPTION

[0031] The specific implementation of the present invention is further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0032] In the description of the present invention, it should be noted that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0033] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0034] Furthermore, in the description of the present invention, unless otherwise specified, “plurality” means two or more.

[0035] Embodiment 1

[0036] like Figures 1 to 6As shown, a closed nucleic acid detection consumable according to a preferred embodiment of the present invention comprises a connector 1, a sample tube 2 and a reaction tube 3. The connector 1 is provided with a sample puncture tube 4 and a reaction puncture tube 5 which are at a certain angle thereto. The sample puncture tube 4 and the reaction puncture tube 5 are located on the same side of the connector 1 and are respectively located at two ends of the connector 1. The connector 1 is provided with a flow channel 101 which connects the sample puncture tube 4 and the reaction puncture tube 5. The connector 1 is provided with a first positioning seat 6 and a second positioning seat 7. The sample puncture tube 4 passes through the first positioning seat 6, the sample tube 2 is plug-connected to the first positioning seat 6, the reaction puncture tube 5 passes through the second positioning seat 7, and the reaction tube 3 is plug-connected to the second positioning seat 7. In this embodiment, a sample puncture tube 4 and a reaction puncture tube 5 are arranged on the connector 1. When the sample tube 2 and the reaction tube 3 are respectively plugged into the first positioning seat 6 and the second positioning seat 7 so that the sample tube 2, the reaction tube 3 and the connector 1 form a whole, the sample puncture tube 4 pierces the sample tube 2 and extends into the sample tube 2, and the reaction puncture tube 5 pierces the reaction tube 3 and extends into the reaction tube 3, so that the sample tube 2 tightened after sampling and the reaction tube 3 containing PCR reaction reagents can be directly connected to the connector 1 without opening the tube cover, thereby realizing a fully closed connection, preventing the sample in the sample tube 2 from leaking out, avoiding infection, and preventing the sample from being contaminated; the sample tube 2, the reaction tube 3 and the connector are connected. After the connection, the solution in the sample tube 2 can enter the flow channel 101 through the sample puncture tube 4 by inversion, shaking or centrifugation, and then the sample solution in the flow channel 101 can flow into the reaction tube 3 by inversion, shaking or centrifugation. Due to the limited space of the flow channel 101, the solution in the sample tube 2 cannot all flow into the flow channel 101, and the sample solution in the sample tube 2 can be quantitatively transferred to the reaction tube 3. After the sample is transferred, the connector 1 is located above the sample tube 2 and the reaction tube 3, and the solution in the sample tube 2 will no longer flow into the reaction tube 3 through the flow channel 101, and the unreacted sample can be retained. When it is necessary to detect again, the sample tube 2 can be removed and taken out for use. The consumables of this embodiment are simple in structure and highly practical, which is conducive to mass production. In this embodiment, the sample puncture tube 4 and the reaction puncture tube 5 are vertically connected to the connector 1.

[0037] Furthermore, the reaction puncture tube 5 of the present embodiment is connected with one end of the flow channel 101, and the connection point between the sample puncture tube 4 and the flow channel 101 is at a certain distance from the other end of the flow channel 101, so that the connection between the sample puncture tube 4 and the flow channel 101 is between the other end of the flow channel 101 to form a quantitative chamber 102. After the connector 1, the sample tube 2 and the reaction tube 3 are connected, when centrifuging, the quantitative chamber 102 is used as the farthest end from the rotation center. Under the action of centrifugal force, the sample solution in the sample tube 2 will be thrown into the quantitative chamber 102 and fill the quantitative chamber 102, so as to achieve quantitative removal of the sample solution, and then all the solution in the quantitative chamber 102 will be transferred to the reaction tube 3 by centrifugation, so as to achieve quantitative transfer of the sample solution.

[0038] Optionally, the sample puncture tube 4 is provided with a main pipeline 401 and a connecting pipeline 402, the main pipeline 401 is connected to the flow channel 101 through the connecting pipeline 402, the diameter of the connecting pipeline 402 is smaller than the diameter of the main pipeline 401, which can prevent the surface tension from blocking the sample solution at the connection between the sample puncture tube 4 and the flow channel 101, and can control the flow rate. In addition, the bottom surface of the flow channel 101 of this embodiment is provided with a leakage groove 103, the leakage groove 103 is located at one end where the reaction puncture tube 5 is connected to the connector 1, and the reaction puncture tube 5 is connected to the leakage groove 103 to prevent the surface tension of the liquid, so that the sample solution can pass through the reaction puncture tube 5 more smoothly and fall into the reaction tube 5. Furthermore, the length of the reaction puncture tube 5 of this embodiment is greater than that of the sample puncture tube 4, which facilitates identification of the connection position of the sample tube 2 and the reaction tube 3, and the sample tube 2 needs to store a throat swab or a nasal swab, and the length of the sample tube 2 is greater than that of the reaction tube 3. When the solution in the sample tube 2 is transferred to the flow channel 101, and inverted, tilted or centrifuged, the solution in the reaction tube 3 can be against the tube mouth of the reaction tube 3 and does not flow into the flow channel 101 from the reaction puncture tube 5, and the solution in the sample tube 2 can smoothly enter the flow channel 101 through the sample puncture tube 4. Optionally, the length of the reaction puncture tube 5 of this embodiment is greater than 0.5 times the length of the reaction tube 3, which can ensure the stock of the reaction reagent and prevent the reaction reagent from flowing into the flow channel 101 when the sample solution is transferred from the sample tube 2 to the flow channel 101.

[0039] In this embodiment, the sample tube 2 is threadedly connected to the first positioning seat 6, and the reaction tube 3 is threadedly connected to the second positioning seat 7, so that when the sample tube 2 is tightened on the first positioning seat 6, the sample puncture tube 4 pierces the sample tube 2 and extends into the sample tube 2, and when the reaction tube 3 is tightened on the second positioning seat 7, the reaction puncture tube 5 pierces the reaction tube 3 and extends into the reaction tube 3. In addition, the side of the connector 1 of this embodiment away from the sample puncture tube 4 and the reaction puncture tube 5 is a transparent plate, which can be used to observe the liquid in the flow channel 101.

[0040] Embodiment 2

[0041] like Figure 7As shown, the difference between this embodiment and the first embodiment is that the sample tube 2 of this embodiment is snap-connected with the first positioning seat 6, and the reaction tube 3 is snap-connected with the second positioning seat 7. The first positioning seat 6 is provided with a snap-lock block 8, and the sample tube 2 is provided with a snap-lock hole 201. When the sample tube 2 is plugged into the first positioning seat 6, the snap-lock block 8 cooperates with the snap-lock hole 201, so that the sample tube 2 can be firmly connected to the first positioning seat 6. The second positioning seat 7 includes two positioning blocks 701, which are arranged at intervals to form a slot for accommodating the end of the reaction tube 3 to be inserted. The positioning blocks 701 are provided with a snap-lock protrusion 702, and one end of the reaction tube 3 is provided with a circle of snap-lock edge 301. When the reaction tube 3 is inserted between the two positioning blocks 701, the snap-lock edge 301 is located between the snap-lock protrusion 702 and the connector 1, so that the reaction tube 3 is firmly connected to the connector 1. The other structures of this embodiment are the same as those of the first embodiment, and will not be repeated here.

[0042] Embodiment 3

[0043] like Figure 8 As shown, the difference between this embodiment and the first embodiment is that the connector 1, sample tube 2 and reaction tube 3 of this embodiment are all provided in plurality, and the plurality of connectors 1 are provided in parallel and sequentially connected to form a whole, so that the plurality of sample tubes 2 are arranged in a row on one side of the whole, and the reaction tubes 3 are arranged in a row on the other side of the whole. It can be used for the detection of multiple samples. The other structures of this embodiment are the same as those of the first embodiment, and will not be repeated here.

[0044] The present invention also provides a closed nucleic acid detection method, comprising the following steps:

[0045] (1) A sample puncture tube 4 and a reaction puncture tube 5 are vertically connected to the two ends of the connector 1, respectively. A flow channel 101 connecting the sample puncture tube 4 and the reaction puncture tube 5 is provided in the connector 1, wherein the reaction puncture tube 5 is connected to one end of the flow channel 101, and a certain distance is provided between the connecting point between the sample puncture tube 4 and the flow channel 101 and the other end of the flow channel 101, so that a quantitative chamber 102 is formed between the connecting point between the sample puncture tube 4 and the flow channel 101 and the other end of the flow channel 101;

[0046] (2) Insert the sample tube 2 containing the nucleic acid sample into the connector 1, insert the sample puncture tube 4 into the sample tube 2, insert the reaction tube 3 containing the nucleic acid amplification reaction reagent into the connector 1, insert the reaction puncture tube 5 into the reaction tube 3, wherein the length of the reaction puncture tube 5 inserted into the reaction tube 3 is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube 3;

[0047] (3) If Fig. 9As shown, the whole after the connector 1, the sample tube 2 and the reaction tube 3 are connected is tilted or placed horizontally, so that the quantitative chamber 102 and the bottom end of the reaction tube 3 are located on the same straight line, and a certain point on the straight line where the quantitative chamber 102 and the reaction tube 3 are located is taken as the first rotation center 9, wherein the bottom end of the reaction tube 3 is closer to the first rotation center 9 than the quantitative chamber 102, and then the whole after the connector 1, the sample tube 2 and the reaction tube 3 are connected is centrifuged around the first rotation center 9, so that the sample tube is transferred to the quantitative chamber;

[0048] (4) If Fig.10 As shown, the entirety of the connector 1, sample tube 2 and reaction tube 3 after being connected is inverted, and a point on the straight line intersecting the connector 1 and passing through the bottom end of the reaction tube 3 is selected as the second rotation center 10. The quantitative chamber 102 is closer to the second rotation center 10 relative to the bottom end of the reaction tube 3. The second rotation center 10, the quantitative chamber 102 and the bottom end of the reaction tube 3 are not located on the same straight line. Then, the entirety of the connector 1, sample tube 2 and reaction tube 3 after being connected is centrifuged around the second rotation center 10 to transfer the liquid in the quantitative chamber to the reaction tube.

[0049] According to the detection method provided by the present invention, after the first centrifugation in step (3), the quantitative sample solution can be retained in the quantitative chamber 102, and the nucleic acid amplification reaction reagent in the reaction tube 3 is gathered at the top of the reaction tube 3. Since the length of the reaction puncture tube 5 inserted into the reaction tube 3 is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube 3, the reaction reagent cannot flood the port of the reaction puncture tube 5, and the reagent will not enter the flow channel 101; after the second centrifugation in step (4), the sample solution in the quantitative chamber 102 enters the reaction tube 3 along the flow channel 101 and the reaction puncture tube 5 under the action of centrifugal force, and is thrown to the bottom of the reaction tube 3 together with the reaction reagent by the centrifugal force, and the sample and the reagent are mixed at the same time. The sample solution in the sample tube 2 also returns to the bottom of the sample tube 2 under the action of centrifugation. After sampling, the sample tube cover 202 is tightened onto the sample tube body 201, and the sample tube cover 202 is no longer opened thereafter, thereby reducing the risk of infection. Finally, the whole body of the connector 1, the sample tube 2 and the reaction tube 3 is placed in a dedicated PCR instrument, and PCR detection can be performed.

[0050] In summary, the embodiment of the present invention provides a closed nucleic acid detection consumable, which arranges a sample puncture tube 4 and a reaction puncture tube 5 on a connector 1. When the sample tube 2 and the reaction tube 3 are respectively plugged into the first positioning seat 6 and the second positioning seat 7 so that the sample tube 2, the reaction tube 3, and the connector 1 form a whole, the sample puncture tube 4 pierces the sample tube 2 and extends into the sample tube 2, and the reaction puncture tube 5 pierces the reaction tube 3 and extends into the reaction tube 3, so that the sample tube 2 tightened after sampling and the reaction tube 3 containing PCR reaction reagents can be directly connected to the connector 1 without opening the tube cover, thereby realizing a fully closed connection, preventing the sample in the sample tube 2 from leaking out, avoiding infection, and preventing the sample from being contaminated; the sample tube 2. After the reaction tube 3 is connected to the connector 1, the solution in the sample tube 2 can be passed through the sample puncture tube 4 into the flow channel 101 by inversion, shaking or centrifugation, and then the sample solution in the flow channel 101 can be flowed into the reaction tube 3 by inversion, shaking or centrifugation. Due to the limited space of the flow channel 101, the solution in the sample tube 2 cannot all flow into the flow channel 101, and the quantitative transfer of the sample solution in the sample tube 2 to the reaction tube 3 can be achieved. After the sample is transferred, the connector 1 is located above the sample tube 2 and the reaction tube 3, and the solution in the sample tube 2 will no longer flow into the reaction tube 3 through the flow channel 101, and the unreacted sample can be retained. When it is necessary to detect again, the sample tube 2 can be removed and taken out for use. The consumables of this embodiment are simple in structure and highly practical, which is conducive to mass production. In addition, the present invention also provides a method for closed nucleic acid detection, wherein the sample solution in the above-mentioned sample tube is transferred to the flow channel of the connector by a first centrifugation, and the sample solution in the flow channel is transferred to the reaction tube by a second centrifugation.

[0051] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and substitutions can be made without departing from the technical principles of the present invention. These improvements and substitutions should also be regarded as the scope of protection of the present invention.

Claims

1. A closed nucleic acid detection consumable, characterized in that: The connector comprises a connector, a sample tube and a reaction tube. The connector is provided with a sample puncture tube and a reaction puncture tube which are at a certain angle thereto. The sample puncture tube and the reaction puncture tube are located on the same side of the connector and are located at two ends of the connector respectively. The connector is provided with a flow channel which connects the sample puncture tube and the reaction puncture tube. The connector is provided with a first positioning seat and a second positioning seat. The sample puncture tube passes through the first positioning seat, and the sample tube is plug-connected with the first positioning seat. The reaction puncture tube passes through the second positioning seat, and the reaction tube is plug-connected with the second positioning seat. The reaction puncture tube is connected to one end of the flow channel, and the connection point between the sample puncture tube and the flow channel is at a certain distance from the other end of the flow channel, so that the connection between the sample puncture tube and the flow channel is located between the other end of the flow channel to form a quantitative chamber; The sample puncture tube is provided with a main pipeline and a connecting pipeline. The main pipeline is communicated with the flow channel through the connecting pipeline. The diameter of the connecting pipeline is smaller than the diameter of the main pipeline.

2. The closed nucleic acid detection consumable according to claim 1, characterized in that: A liquid leakage groove is provided on the bottom surface of the flow channel. The liquid leakage groove is located at one end where the reaction puncture tube is connected to the connector. The reaction puncture tube is in communication with the liquid leakage groove.

3. The closed nucleic acid detection consumable according to claim 1, characterized in that: The sample tube is threadedly connected to the first positioning seat, and the reaction tube is threadedly connected to the second positioning seat.

4. The closed nucleic acid detection consumable according to claim 1, characterized in that: The sample tube is snap-connected to the first positioning seat, and the reaction tube is snap-connected to the second positioning seat.

5. The closed nucleic acid detection consumable according to claim 1, characterized in that: The side of the connector facing away from the sample puncture tube and the reaction puncture tube is a transparent plate.

6. The closed nucleic acid detection consumable according to claim 1, characterized in that: The connector, the sample tube and the reaction tube are all arranged in plurality, and the plurality of connectors are arranged in parallel and sequentially connected to form a whole, so that the plurality of sample tubes are arranged in a row on one side of the whole, and the reaction tubes are arranged in a row on the other side of the whole.

7. A closed nucleic acid detection method, characterized in that: Using the closed nucleic acid detection consumable according to any one of claims 1 to 6, the method comprises the following steps: (1) A sample puncture tube and a reaction puncture tube are vertically connected at both ends of the connector, and a flow channel connecting the sample puncture tube and the reaction puncture tube is provided in the connector, wherein the reaction puncture tube is connected to one end of the flow channel, and a certain distance is provided between the connecting point between the sample puncture tube and the flow channel and the other end of the flow channel, so that a quantitative chamber is formed between the connecting point between the sample puncture tube and the flow channel and the other end of the flow channel; (2) plugging a sample tube containing a nucleic acid sample onto the connector so that the sample puncture tube is inserted into the sample tube, and plugging a reaction tube containing a nucleic acid amplification reaction reagent onto the connector so that the reaction puncture tube is inserted into the reaction tube, wherein the length of the reaction puncture tube inserted into the reaction tube is greater than the height of the nucleic acid amplification reaction reagent in the reaction tube; (3) Tilt or horizontally place the entirety of the connector, the sample tube, and the reaction tube so that the quantitative chamber and the bottom end of the reaction tube are located on the same straight line, and take a certain point on the straight line where the quantitative chamber and the reaction tube are located as the first rotation center, wherein the bottom end of the reaction tube is closer to the first rotation center than the quantitative chamber, and then centrifuge the entirety of the connector, the sample tube, and the reaction tube around the first rotation center so that the liquid in the sample tube is transferred to the quantitative chamber; (4) Invert the entirety of the connector, the sample tube, and the reaction tube after being connected, select a point on a straight line that intersects the connector and passes through the bottom end of the reaction tube as a second rotation center, the quantitative chamber is closer to the second rotation center relative to the bottom end of the reaction tube, and the second rotation center, the quantitative chamber, and the bottom end of the reaction tube are not located on the same straight line, and then centrifuge the entirety of the connector, the sample tube, and the reaction tube after being connected around the second rotation center to transfer the liquid in the quantitative chamber to the reaction tube.

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