Automatic suction device for T lymphocytes

Abstract

The invention belongs to the technical field of inspection and extraction, and particularly relates to an automatic T lymphocyte suction device which comprises an automatic suction device body, the automatic suction device body comprises a bearing table, a rotating disc with a circumferential rotating function is arranged on the bearing table, and a plurality of centrifugal assemblies are evenly distributed on the rotating disc. A centrifugal tube feeding assembly facilitating feeding of centrifugal tubes is arranged in front of one side of the bearing table, a tube carrying and conveying assembly is arranged on the portion, located on one side of the centrifugal tube feeding assembly, of the bearing table, a tube feeding mechanism is arranged at the end of the conveying belt mechanism, and a reaction reagent adding assembly is arranged on one side of the tube carrying and conveying assembly. A sample feeding assembly is arranged on one side of the upper portion of the workbench, and a first suction assembly is arranged above the multi-direction adjusting assembly. According to the invention, multiple samples can be processed in batches, the working efficiency and the cell extraction purity are improved, the influence of manual operation on the result is reduced, the accuracy of the detection effect is ensured, and the use requirements are met.

Description

Technical Field

[0001] The invention belongs to the technical field of testing and extraction, and in particular relates to an automatic T lymphocyte aspiration device. Background Art

[0002] Tuberculosis is a chronic and slow-onset infectious disease that is prone to occur in young people. Mycobacterium tuberculosis infection in the lungs can cause a chronic respiratory infectious disease. Mycobacterium tuberculosis is transmitted to healthy people by pulmonary tuberculosis patients who excrete bacteria through loud talking and coughing droplets.

[0003] The T-cell spot test for tuberculosis infection uses tuberculosis-specific mixed antigens A and B to stimulate the tuberculosis-specific activated T lymphocytes present in the peripheral blood mononuclear cells of tuberculosis-infected patients to secrete gamma interferon. The enzyme-linked immunosorbent assay is then used to detect the number of effector T cells that respond to tuberculosis, thereby providing auxiliary diagnosis for tuberculosis infection. However, in the current test process, there are problems in that sample processing is mainly manual, the operation steps are cumbersome, and the time cost is high. In particular, the purification and extraction of T lymphocytes requires repeated centrifugation. During the separation of T lymphocytes, the influence of human technical operation is significant, which directly determines the quality of the test results. Therefore, in order to address the drawbacks of the above sample processing process, an automatic T lymphocyte aspiration device is provided that can realize batch processing of multiple samples to replace the inconvenience of manual operation one by one, improve work efficiency and cell extraction purity, reduce the influence of human operation on the results, reduce contamination, ensure the accuracy of the test results, and effectively meet the needs of users. Summary of the Invention

[0004] In response to the technical problems existing in the above-mentioned T lymphocyte extraction process, the present invention proposes an automatic T lymphocyte aspiration device with a reasonable design, simple structure, convenient processing, and the ability to process multiple samples in batches, thereby replacing the inconvenience caused by manual operation one by one, improving work efficiency and cell extraction purity, and reducing the impact of human operation on the results, reducing contamination, ensuring the accuracy of detection results, and effectively meeting usage requirements.

[0005] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is an automatic T lymphocyte aspiration device, including an automatic aspiration device body, the automatic aspiration device body including a carrying platform, a workbench is provided on the rear side of the carrying platform, and is characterized in that a rotating disk with a circumferential rotation function is provided on the carrying platform, a plurality of centrifuge components are evenly distributed on the rotating disk, a centrifuge tube loading component for facilitating the loading of centrifuge tubes is provided in front of one side of the carrying platform, a tube-carrying conveying component is provided on the carrying platform on one side of the centrifuge tube loading component, the tube-carrying conveying component includes a conveyor belt mechanism spanning between the centrifuge tube loading component and the rotating disk, a loading shell is placed on the conveyor belt mechanism, and the end of the conveyor belt mechanism The top of the device is provided with an upper tube mechanism, and corresponds to the rotating disk, a reaction reagent adding component is provided on one side of the carrier tube conveying component, a sample loading component is provided on the upper side of the workbench, a material holding mechanism for receiving and placing samples is provided on one side of the sample loading component, and a transfer mechanism integrating material collecting, discharging, opening and external delivery functions is provided on the rear side of the material holding mechanism, a multi-directional adjustment component which can be adjusted horizontally and vertically is provided on one side of the material holding mechanism, a first suction component is provided above the multi-directional adjustment component, a protective component integrating the sealing and unsealing functions is provided on one side of the carrier platform, a screw moving component is provided on one side of the protective component, and a second suction component is provided on the screw moving component.

[0006] Preferably, there are two rotating disks set up above and below, and a circular hole is opened to facilitate the placement of the centrifugal assembly. The centrifugal assembly includes a mounting shell, a driving motor is arranged below the mounting shell, a rotating shell is arranged inside the mounting shell, and is connected to the output end of the driving motor, a plurality of clamping strips are evenly distributed on the inside of the rotating shell, a clamping groove adapted to the clamping strip is opened on the outer periphery of the loading shell, an arc-shaped clamping plate with elastic properties is arranged inside the loading shell, and a centrifuge tube is placed in the middle position between the plurality of clamping plates.

[0007] Preferably, the centrifuge tube loading assembly includes a tube loader, and a first horizontal cylinder capable of longitudinal movement is provided above the output end of the tube loader, a first carrier plate is provided at the output end of the first horizontal cylinder, a first vertical cylinder is provided on the first carrier plate, a lifting rod is provided at the output end of the first vertical cylinder, a second carrier plate is provided at the lower end of the lifting rod, and a first clamping cylinder is provided on the second carrier plate.

[0008] Preferably, one side of the conveyor belt mechanism is placed below the centrifuge tube loading assembly, and the other side is placed on the outside of the rotating disk. The upper tube mechanism includes a loading support frame, a loading support plate is provided above the loading support frame, a first longitudinal cylinder is provided on the loading support plate, a second vertical cylinder is provided at the output end of the first longitudinal cylinder, and a second clamping cylinder is provided below the output end of the second vertical cylinder.

[0009] Preferably, the reaction reagent adding component includes a support frame designed in a concave shape, an inclined sliding rod is provided on one side of the support frame, a sliding seat is provided on the sliding rod, two injection tubes are provided side by side on the sliding seat, a liquid storage box for conveying reaction reagents to the injection tubes is provided above the support frame, a stabilizing support plate is provided below the two sliding rods, a driving cylinder is provided on the stabilizing support plate, and its output end is connected to the sliding seat.

[0010] Preferably, the sample loading assembly includes a conveyor belt assembly, a guide plate with a closing shape from the outside to the inside is provided above the conveyor belt assembly, a transfer tray is provided on one side of the conveyor belt assembly, a plurality of limiting holes are provided on the outside of the transfer tray, and the material holding mechanism includes a stabilizing frame with an open-shaped design, a vertical screw rod assembly is provided in the stabilizing frame, a lifting plate is provided on the outside of the vertical screw rod assembly, a vertical rod is provided on the outside of the lifting plate and passes through the stabilizing frame, a flat plate is provided above the vertical rod, and a bearing tube is provided on the flat plate for bearing and limiting the sample.

[0011] Preferably, the transfer mechanism includes a vertical pole, a sliding limit seat is provided between the two vertical poles, two groups of longitudinally movable and adjustable transfer components are provided on the sliding limit seat, the transfer component includes a moving seat, a third vertical cylinder is provided on the moving seat, the output end of the third vertical cylinder is provided with a cross support plate designed in an L shape, a second horizontal cylinder is provided on the inner side of the cross support plate, a T-shaped plate is provided at the output end of the second horizontal cylinder, a first hollow rotating platform is provided below the T-shaped plate, the output end of the first hollow rotating platform is provided with a connecting plate, and a third clamping cylinder is provided on the connecting plate.

[0012] Preferably, the multi-directional adjustment component includes a synchronous belt longitudinal movement component, a screw horizontal movement component is arranged above the synchronous belt longitudinal movement component, and its output end is connected to the first suction component, the first suction component includes an L-shaped mounting plate, a slide is arranged on the rear side of the mounting plate, and an L-shaped moving plate is arranged on one side of the slide, a fourth vertical cylinder for driving the moving plate is arranged on the mounting plate, a second hollow rotating platform is arranged on one side of the moving plate, a rotating tube is provided at the output end of the second hollow rotating platform, and it passes through the inside of the second hollow rotating platform to the outside of the moving plate, a rotating plate is provided on the outside of the rotating tube, and a fourth clamping cylinder arranged perpendicular to it is provided below the rotating plate, and a kneading mechanism is provided in the rotating plate above the fourth clamping cylinder.

[0013] Preferably, the kneading mechanism includes a stepping motor, the output end of the stepping motor is provided with a driving gear, a driving gear is provided below one side of the driving gear and is meshed with the driving gear, a driven gear is provided on one side of the driving gear and is meshed with the driving gear, the teeth on the driving gear and the driven gear are both arc-shaped, a rotating shaft is provided at the geometric center of the driving gear and the driven gear, an eccentric wheel is provided on the outside of the rotating shaft, and the left and right eccentric wheels are arranged in a mirror image.

[0014] Preferably, the protective component includes a cover-applying machine, a support seat is provided above the output end of the cover-applying machine, a second longitudinal cylinder is provided above the support seat, a third carrier plate is provided at the output end of the second longitudinal cylinder, a fifth vertical cylinder is provided at the third carrier plate, a lifting frame is provided at the output end of the fifth vertical cylinder and passes through the third carrier plate, a suction cup with a suction function is provided below the lifting frame, and a discharge hole is provided in the output end of the cover-applying machine close to the support seat side.

[0015] Compared with the prior art, the advantages and positive effects of the present invention are:

[0016] 1. The present invention provides an automatic T lymphocyte aspiration device. The rotating disk is used to receive centrifuge tubes containing reaction reagents and samples, centrally realize centrifugal processing of the tubes, and circulate them at various processing steps to ensure the smooth progress of the processing work. The sample loading component, transfer mechanism, multi-directional adjustment component and first aspiration component are used. The cooperation between the various equipment components can conveniently place the sample into the centrifuge tube containing the reaction reagent, replacing the manual sampling and placement of the sample, improving the work process and avoiding sample contamination. The protective component is used to provide the prerequisite for the centrifugal processing of the sample, ensuring the use requirements. The second aspiration component is used to conveniently aspirate the required sample layer in the centrifuge tube after the reaction and output it to a designated location, improving the work process. The device has a reasonable design, simple structure, easy processing and can realize batch processing of multiple samples, replacing the inconvenience of manual operation one by one, improving work efficiency and cell extraction purity, and reducing the impact of human operation on the results, reducing contamination, ensuring the accuracy of the detection effect, and effectively meeting the use requirements. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0018] Figure 1 This is a schematic diagram of the structure of the automatic T lymphocyte aspiration device;

[0019] Figure 2 It is a structural schematic diagram of the centrifuge tube feeding assembly;

[0020] Figure 3 It is a schematic diagram of the structure of the sample loading assembly;

[0021] Figure 4 is a structural schematic diagram of the first suction component;

[0022] Figure 5 This is a structural diagram of the first suction component from another perspective;

[0023] Figure 6 It is a structural diagram of the protection component;

[0024] Figure 7 is a structural schematic diagram of the second suction component;

[0025] Figure 8 It is a structural schematic diagram of the centrifugal assembly;

[0026] Figure 9 It is a front view of the internal structure of the loading shell;

[0027] In the above figures, 1, carrier platform; 2, workbench; 3, rotating disk; 4, centrifugal assembly; 41, mounting shell; 42, drive motor; 43, rotating shell; 431, clamping strip; 44, centrifugal tube; 5, centrifugal tube loading assembly; 51, tube loading machine; 52, first horizontal cylinder; 53, first carrier plate; 54, first vertical cylinder; 55, lifting rod; 56, second carrier plate; 57, first clamping cylinder; 6, conveyor belt mechanism; 7, loading shell; 71, clamping groove; 72, pressing plate; 8, tube loading mechanism; 81, loading support frame; 82, loading support plate; 83, first longitudinal 84, second vertical cylinder; 85, second gripping cylinder; 9, reaction reagent adding assembly; 91, support frame; 92, slide bar; 93, slide seat; 94, syringe; 95, liquid storage box; 96, stabilizing support plate; 97, driving cylinder; 10, sample loading assembly; 101, conveyor belt assembly; 102, guide plate; 103, transfer tray; 1031, limiting hole; 11, material holding mechanism; 111, stabilizing frame; 112, vertical screw rod assembly; 113, lifting plate; 114, vertical rod; 115, flat plate; 116, carrying tube; 12, transfer mechanism; 121. Vertical pole; 122. Sliding limit seat; 123. Transfer assembly; 1231. Moving seat; 1232. Third vertical cylinder; 1233. Cross brace; 1234. Second horizontal cylinder; 1235. T-shaped plate; 1236. First hollow rotating platform; 1237. Connecting plate; 1238. Third clamping cylinder; 13. Multi-directional adjustment assembly; 131. Synchronous belt longitudinal movement assembly; 132. Screw rod horizontal movement assembly; 14. First suction assembly; 141. Mounting plate; 1411. Slide; 142. Moving plate; 143. Fourth vertical cylinder; 1 44. Second hollow rotating platform; 145. Rotating tube; 146. Rotating plate; 147. Fourth clamping cylinder; 15. Protective assembly; 151. Covering machine; 1511. Feeding hole; 152. Support seat; 153. Second longitudinal cylinder; 154. Third carrier plate; 155. Fifth vertical cylinder; 156. Lifting frame; 157. Suction cup; 16. Screw moving assembly; 17. Second suction assembly; 18. Kneading mechanism; 181. Stepping motor; 182. Driving gear; 183. Active gear disc; 184. Driven gear disc; 185. Rotating shaft; 186. Eccentric wheel. DETAILED DESCRIPTION

[0028] In order to more clearly understand the above-mentioned objects, features and advantages of the present invention, the present invention is further described below in conjunction with the accompanying drawings and embodiments. It should be noted that, in the absence of conflict, the embodiments of the present application and the features therein can be combined with each other.

[0029] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways than those described herein. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0030] Examples, such as Figures 1 to 9 As shown, a T lymphocyte automatic aspiration device includes an automatic aspiration device body, the automatic aspiration device body includes a carrying platform 1, a workbench 2 is provided on the rear side of the carrying platform 1, a rotating disk 3 with a circumferential rotation function is provided on the carrying platform 1, and a plurality of centrifugal components 4 are evenly distributed on the rotating disk 3. A centrifuge tube loading component 5 for facilitating the loading of centrifuge tubes 44 is provided in front of one side of the carrying platform 1, a tube conveying component is provided on the carrying platform 1 on one side of the centrifuge tube loading component 5, and the tube conveying component includes a conveying component spanning between the centrifuge tube loading component 5 and the rotating disk 3. The conveyor belt mechanism 6 is provided with a loading shell 7 on the conveyor belt mechanism 6, and the interior thereof is used to place a centrifuge tube 44, providing convenient conditions for the storage of samples and reaction reagents. The end of the conveyor belt mechanism 6 is provided with a tube mechanism 8, which corresponds to the rotating disk 3. A reaction reagent adding component 9 is provided on one side of the tube transport component. A sample loading component 10 is provided on one side of the workbench 2. A material receiving mechanism 11 for receiving and placing the sample is provided on one side of the sample loading component 10. The rear side of the material receiving mechanism 11 is provided with a transport mechanism 1 that integrates the functions of collecting, unloading, opening the cover and delivering the sample. 2. A multi-directional adjustment component 13 that can be adjusted horizontally and vertically is provided on one side of the material holding mechanism 11. A first suction component 14 is provided above the multi-directional adjustment component 13. A protective component 15 that integrates the functions of sealing and unsealing is provided on one side of the carrier 1. A screw moving component 16 is provided on one side of the protective component 15. A second suction component 17 is provided on the screw moving component 16. The specific components of the second suction component 17 are the same as those of the first suction component 14. Their functions are to first clamp the disposable medical straw, The air inside the rubber head is expelled by simulating manual kneading, and then the straw is placed in the target material to be sucked, and then the kneading mechanism 18 is used to loosen it to complete the corresponding suction action. When the target material is stored in the straw, it is placed in the appropriate position according to the next working process, and then the kneading mechanism 18 is controlled to simulate kneading to complete the discharge of the target material, ensuring the working process. As for the screw cylinder assembly, it provides a prerequisite for driving the second suction assembly 17 to move closer to or away from the rotating disk 3 to achieve the suction or discharge of the material, ensuring the working process;

[0031] The specific work flow is described as follows: when using the automatic aspiration device body mentioned in this embodiment to carry out the corresponding T lymphocyte aspiration work, the equipment needs to be placed in a sterile environment that complies with the processing flow to prevent the possibility of external factors affecting the test results. When in use: a certain amount of centrifuge tubes 44 are placed in the centrifuge tube loading assembly 5, and the centrifuge tube loading assembly 5 is used to arrange multiple centrifuge tubes 44 neatly, and with the help of the first clamping cylinder 57, they are placed in the loading shell 7 of the conveyor belt mechanism 6, and then the conveyor belt mechanism 6 transports the loading shell 7 and the centrifuge tube 44. When it moves to the position of the reaction reagent adding assembly 9, the conveyor belt mechanism 6 stops conveying and waits for the reaction reagent to be injected into the centrifuge tube 44. After the reaction reagent is added, the conveyor is The belt mechanism 6 continues to run, and when it moves to the upper tube mechanism 8, the upper tube mechanism 8 transports the above components together to the centrifugal assembly 4 of the rotating disk 3, and then controls the rotating disk 3 to rotate, and transports the centrifuge tube 44 containing the reaction reagent to a position close to the first suction assembly 14, and under the action of the multi-directional adjustment assembly 13, it realizes adjustment at different positions. During the rotation of the above-mentioned centrifuge tube 44, the sample loading assembly 10 receives the sample tube and inputs it into the rotating disk 103. Relying on its rotation, it drives the sample to move toward a position close to the material holding mechanism 11. When the sample tube arrives at the position close to the material holding mechanism 11, the transfer mechanism 12 is controlled to operate so that it clamps the sample tube and cooperates with another transfer mechanism 12 to realize The end caps of the sample tubes are removed and placed in the material holding mechanism 11. The front transport mechanism 12 continues to perform the clamping action, and the rear transport mechanism 12 first outputs the end caps outward. In this process, after the first suction component 14 completes the suction of the sample under the action of the multi-directional adjustment component 13, the rear transport mechanism 12 outputs the sample tube outward. Of course, the above-mentioned export position is a centralized position to avoid contamination. The first suction component 14 containing the sample moves toward the rotating disk 3 and places the sample in the centrifuge tube 44. It then puts the disposable straw into a unified position and clamps a new disposable straw from the outside, waiting for the next processing step. The centrifuge tube 44 containing the sample and reaction reagent continues to rotate with the rotating disk 3 until it is close to the rotating disk 3. At the position near the protective component 15, the upper end of the centrifuge tube 44 is sealed by the protective component 15. After the sealing work is completed, centrifugal processing is carried out. After the centrifugal processing is completed, the end cover is removed by the protective component 15 and collected and guided to a unified position. The centrifuge tube 44 after centrifugation continues to rotate. When it rotates to a position close to the second suction component 17, the screw moving component 16 drives the second suction component 17 to approach the rotating disk 3, and inserts the suction pipe into the centrifuge tube 44 in an inclined manner, and corresponds to the target material layer. Repeat the simulated manual kneading action to achieve the suction of the target material and ensure the working process. After the target material layer is sucked, the screw moving component 16 is controlled to adjust the position of the second suction component 17.The target material layer is output to the designated collection position. As for the centrifuge tube 44 after the target material layer is taken out, it continues to rotate. After it rotates to the idle position of the loading platform, a manipulator can be set up outside to take out the loading shell 7 and the centrifuge tube 44 in the centrifuge assembly 4 together. The centrifuge assembly 4 continues to work. Under the influence of the inside of the loading shell 7, after it is taken out as a whole, it can press down on the top of the centrifuge tube 44 to separate it from the loading shell 7, and the centrifuge tube 44 is collected centrally. The loading shell 7 can be transported back to the conveyor belt mechanism 6 to realize a closed processing loop, which greatly improves the functionality of the device and meets the use requirements.

[0032] In the above process, the rotating disk 3 is used to receive the centrifuge tube 44 containing the reaction reagents and samples, and the centrifugal treatment thereof is centralized and transferred to each processing step to ensure the smooth progress of the processing. The sample loading assembly 10, the transfer mechanism 12, the multi-directional adjustment assembly 13 and the first suction assembly 14 are used to cooperate with each other to conveniently place the sample into the centrifuge tube 44 containing the reaction reagent, replacing the manual sampling and placement of the sample, thereby improving the work process and avoiding contamination of the sample. The protective assembly 15 is used to provide the prerequisite for the centrifugal processing of the sample and ensure the use requirements. The second suction assembly 17 is used to conveniently absorb the required sample layer in the centrifuge tube 44 after the reaction and output it to a designated location, thereby improving the work process. The device has a reasonable design, a simple structure, and is easy to process. It can realize batch processing of multiple samples, replacing the inconvenience of manual operation one by one, improving work efficiency and cell extraction purity, and reducing the impact of human operation on the results, reducing contamination, ensuring the accuracy of the detection effect, and effectively meeting the use requirements.

[0033] The centrifugal assembly 4 is provided with a plurality of springs 46 on its upper and lower sides, and a plurality of springs 47 are provided on its lower side to facilitate the installation of the centrifugal assembly 4. The centrifugal assembly 4 is provided with a plurality of springs 46 on its upper and lower sides, and a plurality of springs 47 are provided on its lower and upper sides to facilitate the installation of the centrifugal assembly 4. The centrifugal assembly 4 is provided with a plurality of springs 46 on its upper and lower sides, and a plurality of springs 47 are provided on its upper and lower sides to facilitate the installation of the centrifugal assembly 4 The limiting of the material shell 7 ensures the stability of its installation and provides convenient conditions for the subsequent centrifugal work. An arc-shaped and elastic clamping plate 72 is provided in the loading shell 7, and a centrifuge tube 44 is placed in the middle position between multiple clamping plates 72. Specifically, when the centrifuge tube 44 is placed in the loading shell 7, the clamping plate 72 is deformed and can limit and clamp the position of the centrifuge tube 44 to a certain extent, reducing the possibility of its displacement. At the same time, a through hole corresponding to the centrifuge tube 44 is opened at the bottom of the loading shell 7. After the T lymphocyte layer in the centrifuge tube 44 is absorbed, the loading shell 7 can be taken out of the centrifuge assembly 4 by an external manipulator, and then the centrifuge tube 44 is pressed down. In this way, the centrifuge tube 44 can be placed in a unified collection position, and the loading shell 7 can be repeatedly input onto the conveyor belt mechanism 6 to meet the subsequent processing and use requirements, thereby realizing a closed loop of processing work, simple and convenient operation, and strong functionality.

[0034] In order to realize the convenient loading of the centrifuge tube 44, the centrifuge tube loading assembly 5 includes a tube loading machine 51. The tube loading machine 51 is an existing mature and conventional technical means. Its function is to arrange a batch of centrifuge tubes 44 in sequence to ensure the smooth progress of its subsequent loading work. Further, a first horizontal cylinder 52 capable of longitudinal movement is provided above the output end of the tube loading machine 51, and a first carrier plate 53 is provided at the output end of the first horizontal cylinder 52. A first vertical cylinder 54 is provided on the first carrier plate 53. For the establishment of the first horizontal cylinder 52 and the first vertical cylinder 54 established above, it refers to that they can be extended and retracted in the horizontal or vertical direction respectively to ensure that they can realize the driving action in the corresponding direction to meet the use requirements. The output end of the first vertical cylinder 54 is provided There is a lifting rod 55, and a second carrier plate 56 is provided at the lower end of the lifting rod 55. A first clamping cylinder 57 is provided on the second carrier plate 56. For the clamping cylinder set up, it can be a finger cylinder or other cylinders with clamping function. The clamping of its output end is used to clamp the corresponding workpiece to ensure the working process. When in use, the position of the first clamping cylinder 57 can be adjusted by using the first horizontal cylinder 52 set up to make it correspond to the lead-out position of the centrifuge tube 44, and then the first vertical cylinder 54 is controlled to operate, driving the first clamping cylinder 57 to move downward and clamp the centrifuge tube 44, and then the first horizontal cylinder 52 is used to adjust the position of the centrifuge tube 44 and place it in the loading shell 7, providing a prerequisite for subsequent centrifugal processing.

[0035] In order to put the centrifuge tube 44 containing the reaction reagent into the centrifuge assembly 4 in the rotating disk 3, one side of the conveyor belt mechanism 6 is placed below the centrifuge tube loading assembly 5, and the other side is placed on the outside of the rotating disk 3. The upper tube mechanism 8 includes a loading support frame 81, and a loading support plate 82 is provided above the loading support frame 81. A first longitudinal cylinder 83 is provided on the loading support plate 82. It is worth noting that the first longitudinal cylinder 83 can be extended and retracted in the longitudinal direction to ensure the effective implementation of the corresponding action. The output end of the first longitudinal cylinder 83 A second vertical cylinder 84 is provided, and a second clamping cylinder 85 is provided below the output end of the second vertical cylinder 84. The specific description is: the first longitudinal cylinder 83 is used to adjust its longitudinal position, that is, to switch between the rotating disk 3 and the conveyor belt mechanism 6. The second vertical cylinder 84 is used to conveniently clamp the workpiece on the conveyor belt mechanism 6 and place it on the rotating disk 3. The operation is simple and convenient, which realizes the convenient transfer of workpieces between multiple devices, ensures the smooth progress of processing work, and meets the use requirements.

[0036] In order to facilitate the addition of reaction reagents to the centrifuge tube 44, the reaction reagent adding component 9 includes a support frame 91 with a concave shape, a sliding rod 92 arranged obliquely is provided on one side of the support frame 91, a sliding seat 93 is provided on the sliding rod 92, and two injection tubes 94 are arranged side by side on the sliding seat 93. A liquid storage tank 95 for transporting reaction reagents to the injection tubes 94 is provided above the support frame 91. Of course, a pump body is also provided between the liquid storage tank 95 and the injection tube 94 to facilitate the extraction of the reaction reagents. The lower part of the two sliding rods 92 is provided with a liquid storage tank 95 for transporting reaction reagents to the injection tubes 94. A stabilizing support plate 96 is provided on the side, and a driving cylinder 97 is provided on the stabilizing support plate 96, and its output end is connected to the sliding seat 93. The specific description is: when the conveyor belt mechanism 6 drives the loading shell 7 containing the centrifuge tube 44 to be transported to the position near the reaction reagent adding component 9, the driving cylinder 97 is controlled to operate, so that it contracts and drives the injection tube 94 to move obliquely downward into the centrifuge tube 44. After adjusting to the appropriate position, the pump body is controlled to operate to inject the reaction reagent into the centrifuge tube 44, providing convenient conditions for the subsequent placement of samples and ensuring the work progress.

[0037] In order to realize convenient loading of sample tubes, the sample loading assembly 10 includes a conveyor belt assembly 101, and a guide plate 102 with a closing shape from the outside to the inside is provided above the conveyor belt assembly 101, wherein the gap between the two guide plates 102 corresponds to the specifications of the sample tubes, and rows of sample tubes are placed on the conveyor belt assembly 101, and its conveying function is utilized to transport each sample tube to the rotating material tray 103, and then the subsequent transfer mechanism 12 is relied upon to take the material, so as to ensure the smooth progress of the sampling work. A rotating material tray 103 is provided on one side of the conveyor belt assembly 101, and a plurality of limiting holes 1031 are provided on the outer side of the rotating material tray 103. The specifications of the limiting holes 1031 are adapted to the sample tubes, so as to ensure that each sample tube can be stably rotated and output; in order to ensure convenient absorption of samples in the sample tubes, the material holding mechanism 11 includes a stable frame designed in the shape of an open letter. 111. A vertical screw rod assembly 112 is provided in the stabilizing frame 111, and a lifting plate 113 is provided on the outside of the vertical screw rod assembly 112. A vertical rod 114 is provided on the outside of the lifting plate 113 and passes through the stabilizing frame 111. A flat plate 115 is provided above the vertical rod 114. A carrying tube 116 is provided on the flat plate 115 for carrying and limiting the sample. Specifically, the carrying tube 116 is used to receive the sample tube that has been transported to ensure the stability of its placement position. After the sample tube is placed in the carrying tube 116, the first suction component 14 moves to the top near the material holding mechanism 11, and then the vertical screw rod assembly 112 is used to adjust the vertical position of the carrying tube 116, so that it rises slowly and cooperates with the first suction component 14 to complete the suction of the sample. The operation is simple and convenient, and the use functionality is strong.

[0038] In order to facilitate the placement of the sample tube from the sample loading component 10 into the material holding mechanism 11 and to facilitate the processing of the sample tube after the sample is sucked, the transport mechanism 12 includes a vertical rod 121, a sliding limit seat 122 is provided between the two vertical rods 121, and two groups of longitudinally movable and adjustable transport components 123 are provided on the sliding limit seat 122. The transport component 123 includes a moving seat 1231, which is engaged with the sliding limit seat 122 in a snap-fit manner, thereby ensuring the stability of the moving seat 1231 and ensuring its smoothness during movement. A third vertical cylinder 1232 is provided on the moving seat 1231. The output end of the cylinder 1232 is provided with a horizontal support plate 1233 of L-shaped design, and a second horizontal cylinder 1234 is provided on the inner side of the horizontal support plate 1233. The output end of the second horizontal cylinder 1234 is provided with a T-shaped plate 1235. A first hollow rotating platform 1236 is provided below the T-shaped plate 1235. The output end of the first hollow rotating platform 1236 is provided with a connecting plate 1237. The third clamping cylinder 1238 is provided on the connecting plate 1237. The specific description is as follows: after the conveyor belt assembly 101 transports the sample to the rotating material tray 103, as the rotating material tray 103 rotates, it gradually moves to the position close to the material holding mechanism 11. Position, when a sample reaches the above position, the transport assembly 123 on the right side is operated, and the cooperation between the third vertical cylinder 1232, the second horizontal cylinder 1234 and the third clamping cylinder 1238 is used to clamp the sample tube and move it to a position close to the carrying mechanism. Since the sample tube generally has an end cap, another transport assembly 123 is used to control its third clamping cylinder 1238 to approach the end cap of the sample tube and clamp it, and respectively control the two first hollow rotating platforms 1236 to rotate, and the two rotation directions are opposite, so as to remove the end cap of the sample tube. After the end cap is removed, the transport assembly holding the end cap is clamped. 123 outputs the end cap to the outside, and another transfer component 123 places the sample tube on the material holding mechanism 11, waiting for the sample to be absorbed. After the transfer component 123 places the sample tube on the material holding mechanism 11, it performs the work of clamping the next sample tube. At this time, the previous transfer component 123 waits for the completion of sample absorption after outputting the end cap to the designated position, and then clamps the sample tube after sampling, and outputs it to the external centralized position again. This reciprocating process not only realizes the convenient loading of the sample tube, but also can output each tool to the designated position in a classified manner, greatly improving the functionality of the device and ensuring usage needs.

[0039] In order to realize convenient suction of samples and ensure that its working process complies with the operating specifications, the multi-directional adjustment component 13 includes a synchronous belt longitudinal movement component 131. As for the synchronous belt longitudinal movement component 131, it includes two driving wheels, and a synchronous belt is set between the two driving wheels. The two ends of the synchronous belt are respectively connected to the bottom of the screw rod transverse movement component 132. In this way, the driving wheel drives the synchronous belt to rotate and act on the screw rod transverse movement component 132 to realize convenient adjustment of its longitudinal position. A screw rod transverse movement component 132 is set above the synchronous belt longitudinal movement component 131, and its output end is connected to the first suction component 14. The establishment of the screw rod transverse movement component 132 utilizes the rotation of the ball screw in the prior art to realize the translational movement of the equipment. The above-mentioned devices are all existing mature and conventional technical means, and the specific working principle will not be repeated. In this embodiment, the cooperation between the above-mentioned two devices can, on the one hand, facilitate the first suction component 14 to clamp the external disposable medical pipette and put it into the sample suction. During the sampling process, the sample after sampling is output to the centrifuge tube 44. On the other hand, the disposable straw that has completed the work can be output to the outside, waiting for the subsequent clamping of a new disposable straw, thereby ensuring the relative sterility during the sample processing process and the accuracy of the test results. Furthermore, the first suction component 14 includes an L-shaped mounting plate 141, wherein a hollow rotating platform is also provided between the bottom of the mounting plate 141 and the output end of the screw lateral movement component 132, for adjusting the circumferential position of the first suction component 14 to meet the needs of different processing operations. For example, when clamping a disposable medical straw from the outside, the fourth clamping cylinder 147 faces outward. When the sample is sucked from the sample tube, its position is adjusted to the material holding mechanism 11 by rotation. When the sample needs to be discharged, the fourth clamping cylinder 147 can be rotated to face the rotating disk 3. Of course, the fourth clamping cylinder 147 can also be not directed toward the rotating disk 3. When the sample is output, it is only necessary to ensure that the second hollow rotating platform 144 can tilt the straw.A slide bar 1411 is provided on the rear side of the mounting plate 141, which limits the lifting direction of the movable plate 142 and ensures its smoothness during movement. A movable plate 142 with an L-shaped design is provided on one side of the slide bar 1411, and a fourth vertical cylinder 143 for driving the movable plate 142 is provided on the mounting plate 141. A second hollow rotating platform 144 is provided on one side of the movable plate 142, and a rotating tube 145 is provided at the output end of the second hollow rotating platform 144, and passes through the inside of the second hollow rotating platform 144 to the outside of the movable plate 142, a rotating plate 146 is provided on the outside of the rotating tube 145, and a fourth clamping cylinder arranged perpendicular to the rotating plate 146 is provided below the rotating plate 146 147. The kneading mechanism 18 is located within the rotating plate 146 above the fourth clamping cylinder 147. Specifically, the extension and retraction of the fourth vertical cylinder 143 is used to drive the movable plate 142 to different vertical positions, facilitating its aspiration of samples from the sample tube. The second hollow rotating platform 144 is configured to adjust the vertical angles between the fourth clamping cylinder 147 and the kneading mechanism 18. This allows the lower end of the fourth clamping cylinder 147 to tilt and contact the sidewall of the sample tube or centrifuge tube 44 when clamping a medical pipette. This complies with existing operating technical specifications, ensures convenient sample aspiration and delivery, and safeguards the work process.

[0040] In order to ensure the effective implementation of the suction action, the kneading mechanism 18 includes a stepping motor 181, and the output end of the stepping motor 181 is provided with a driving gear 182, and a driving gear 183 meshing with it is provided on the lower side of the driving gear 182, and a driven gear 184 meshing with it is provided on one side of the driving gear 183. The teeth on the driving gear 183 and the driven gear 184 are both arc-shaped, and the geometric centers of the driving gear 183 and the driven gear 184 are provided with a rotating shaft 185. The outer side of the rotating shaft 185 is provided with an eccentric wheel 186, and the left and right eccentric wheels 186 are arranged in a mirror image. The specific description is: when the fourth clamping cylinder 147 in the first suction component 14 clamps the disposable medical straw, the top of the medical straw, that is, the rubber head of the straw, corresponds to the position of the kneading mechanism 18, and the first Before the suction component 14 enters the sample tube, the stepper motor 181 is first controlled to operate, the driving gear 182 rotates and acts on the active gear plate 183, and the driven gear plate 184 engages with it, thereby driving the rotating shaft 185 to rotate, and further acts on the eccentric wheel 186. The big heads of the left and right eccentric wheels 186 are close to each other and act on the rubber head to discharge the internal air. When the pipette enters the sample tube, the stepper motor 181 is controlled to operate in the reverse direction, and the big head of the eccentric wheel 186 rotates outward, so that the sample in the sample tube can be sucked. After the sample is transported to the centrifuge tube 44 with the pipette, the stepper motor 181 is controlled to operate again to perform the air discharge action, that is, the sample can be output to the centrifuge tube 44, providing convenient conditions for the subsequent centrifugal processing of the sample and reagent, and ensuring usage requirements.

[0041] In order to ensure the convenience of centrifugal tube 44 in carrying out centrifugal work and avoid the possibility of material splashing and causing waste, the protective component 15 includes a cover machine 151, a support 152 is provided above the output end of the cover machine 151, a second longitudinal cylinder 153 is provided above the support 152, a third carrier plate 154 is provided at the output end of the second longitudinal cylinder 153, a fifth vertical cylinder 155 is provided on the third carrier plate 154, a lifting frame 156 is provided at the output end of the fifth vertical cylinder 155, and passes through the third carrier plate 154, and a lifting frame 156 is provided below the lifting frame 156. A suction cup 157 with a suction function is provided, and a discharge hole 1511 is provided in the output end of the capping machine 151 near the support 152. Specifically, the protection component 15 is established in a similar manner to the working principle of the centrifuge tube loading component 5. The capping machine 151 in the prior art is used to deliver the cap that can be adapted to the upper tube mouth of the centrifuge tube 44, so that it can be placed on or detached from the centrifuge tube 44 in a plug-in manner. That is, the tube loading machine 51 delivers each cap to its output end, and uses the second longitudinal cylinder 153 to adjust the longitudinal position of the suction cup 157. , and then use the operation of the fifth vertical cylinder 155 to make the suction cup 157 suck the cover, and then under the action of the second longitudinal cylinder 153, make the cover close to the centrifuge tube 44, and with the help of the fifth vertical cylinder 155, press the cover on the centrifuge tube 44 to complete the sealing of the tube, ensuring the smooth progress of subsequent centrifugation. When the centrifugation is completed, adjust the suction effect of the suction cup 157, pull the cover out from above the centrifuge tube 44 after centrifugation, and then, with the cooperation of each cylinder, guide the used cover out from the discharge hole 1511. Of course, a material holding box or an inclined discharge plate can be installed below the discharge hole 1511 to collect the used caps in a centralized manner and improve the work process. Of course, for the above-mentioned caps, when a screw-type is selected, a clamping and rotating mechanism can be added to one side of the suction cup 157. Of course, the structure of the clamping and rotating mechanism is also an existing mature and commonly used technical means. The specific working principle will not be repeated. After the suction cup 157 places the cap on the centrifuge tube 44, the cap can be disassembled and assembled with the help of this structure to ensure the work process and meet the use requirements.

[0042] The above description is merely a preferred embodiment of the present invention and does not constitute any other form of limitation to the present invention. Any person skilled in the art may utilize the technical contents disclosed above to change or modify them into equivalent embodiments with equivalent changes for application in other fields. However, any simple modification, equivalent change, and modification of the above embodiments made in accordance with the technical essence of the present invention without departing from the technical solution of the present invention shall still fall within the scope of protection of the technical solution of the present invention.

Claims

1. A T lymphocyte automatic aspiration device, comprising an automatic aspiration device body, the automatic aspiration device body comprising a carrying platform, a workbench being provided on the rear side of the carrying platform, characterized in that: The carrier platform is provided with a rotating disk with a circumferential rotation function, and a plurality of centrifuge components are evenly distributed on the rotating disk. A centrifuge tube loading component is provided in front of one side of the carrier platform for loading centrifuge tubes. A tube conveying component is provided on the carrier platform located on one side of the centrifuge tube loading component. The tube conveying component includes a conveyor belt mechanism spanning between the centrifuge tube loading component and the rotating disk, a loading shell is placed on the conveyor belt mechanism, and a tube loading mechanism is provided at the end of the conveyor belt mechanism, and corresponds to the rotating disk. A reaction reagent adding component is provided on one side of the tube conveying component. A sample loading assembly is provided on one side above the workbench, a material holding mechanism for receiving and placing samples is provided on one side of the sample loading assembly, a transfer mechanism integrating material collecting, material discharging, lid opening and external delivery functions is provided on the rear side of the material holding mechanism, a multi-directional adjustment assembly that can be adjusted horizontally and vertically is provided on one side of the material holding mechanism, a first suction assembly is provided above the multi-directional adjustment assembly, a protective assembly integrating lid sealing and lid removing functions is provided on one side of the supporting platform, a screw moving assembly is provided on one side of the protective assembly, and a second suction assembly is provided on the screw moving assembly.

2. The automatic T lymphocyte aspiration device according to claim 1, characterized in that: There are two rotating disks set up above and below, and a circular hole is opened to facilitate the placement of the centrifugal assembly. The centrifugal assembly includes a mounting shell, a driving motor is arranged below the mounting shell, a rotating shell is arranged inside the mounting shell, and is connected to the output end of the driving motor, a plurality of clamping strips are evenly distributed on the inside of the rotating shell, and a clamping groove adapted to the clamping strip is opened on the outer periphery of the loading shell, and an arc-shaped clamping plate with elastic properties is arranged inside the loading shell, and a centrifuge tube is placed in the middle position between the plurality of clamping plates.

3. The automatic T lymphocyte aspiration device according to claim 2, characterized in that: The centrifuge tube loading assembly includes a tube loader, and a first horizontal cylinder capable of longitudinal movement is provided above the output end of the tube loader, a first carrier plate is provided at the output end of the first horizontal cylinder, a first vertical cylinder is provided on the first carrier plate, a lifting rod is provided at the output end of the first vertical cylinder, a second carrier plate is provided at the lower end of the lifting rod, and a first clamping cylinder is provided on the second carrier plate.

4. The automatic T lymphocyte aspiration device according to claim 3, characterized in that: One side of the conveyor belt mechanism is placed below the centrifuge tube loading assembly, and the other side is placed on the outside of the rotating disk. The upper tube mechanism includes a loading support frame, a loading support plate is provided above the loading support frame, a first longitudinal cylinder is provided on the loading support plate, a second vertical cylinder is provided at the output end of the first longitudinal cylinder, and a second clamping cylinder is provided below the output end of the second vertical cylinder.

5. The automatic T lymphocyte aspiration device according to claim 4, characterized in that: The reaction reagent adding component includes a support frame designed in a concave shape, an inclined sliding rod is provided on one side of the support frame, a sliding seat is provided on the sliding rod, two injection tubes are provided side by side on the sliding seat, a liquid storage box for transporting reaction reagents to the injection tubes is provided above the support frame, a stabilizing support plate is provided below the two sliding rods, a driving cylinder is provided on the stabilizing support plate, and its output end is connected to the sliding seat.

6. The automatic T lymphocyte aspiration device according to claim 5, characterized in that: The sample loading assembly includes a conveyor belt assembly, a guide plate with a closing shape from the outside to the inside is provided above the conveyor belt assembly, a rotating material tray is provided on one side of the conveyor belt assembly, and a plurality of limiting holes are provided on the outer side of the rotating material tray, and the material holding mechanism includes a stabilizing frame with an open-shaped design, a vertical screw rod assembly is provided in the stabilizing frame, a lifting plate is provided on the outer side of the vertical screw rod assembly, a vertical rod is provided on the outer side of the lifting plate and passes through the stabilizing frame, a flat plate is provided above the vertical rod, and a bearing tube for bearing and limiting the sample is provided on the flat plate.

7. The automatic T lymphocyte aspiration device according to claim 6, characterized in that: The transfer mechanism includes a vertical pole, a sliding limit seat is provided between the two vertical poles, two groups of longitudinally movable and adjustable transfer components are provided on the sliding limit seat, the transfer component includes a moving seat, a third vertical cylinder is provided on the moving seat, the output end of the third vertical cylinder is provided with a cross brace designed in an L shape, a second horizontal cylinder is provided on the inner side of the cross brace, a T-shaped plate is provided at the output end of the second horizontal cylinder, a first hollow rotating platform is provided below the T-shaped plate, the output end of the first hollow rotating platform is provided with a connecting plate, and a third clamping cylinder is provided on the connecting plate.

8. The automatic T lymphocyte aspiration device according to claim 7, characterized in that: The multi-directional adjustment component includes a synchronous belt longitudinal movement component, a screw rod transverse movement component is provided above the synchronous belt longitudinal movement component, and its output end is connected to the first suction component, the first suction component includes an L-shaped mounting plate, a slide is provided on the rear side of the mounting plate, and an L-shaped movable plate is provided on one side of the slide, a fourth vertical cylinder for driving the movable plate is provided on the mounting plate, a second hollow rotating platform is provided on one side of the movable plate, a rotating tube is provided at the output end of the second hollow rotating platform, and passes through the inside of the second hollow rotating platform to the outside of the movable plate, a rotating plate is provided on the outside of the rotating tube, and a fourth clamping cylinder arranged perpendicular to it is provided below the rotating plate, and a kneading mechanism is provided in the rotating plate above the fourth clamping cylinder.

9. The automatic T lymphocyte aspiration device according to claim 8, characterized in that: The kneading mechanism includes a stepping motor, an output end of the stepping motor is provided with a driving gear, a driving gear is provided below one side of the driving gear and is meshed with the driving gear, a driven gear is provided on one side of the driving gear and is meshed with the driving gear, the teeth on the driving gear and the driven gear are both arc-shaped, a rotating shaft is provided at the geometric center of the driving gear and the driven gear, an eccentric wheel is provided on the outside of the rotating shaft, and the left and right eccentric wheels are arranged in a mirror image.

10. The automatic T lymphocyte aspiration device according to claim 9, characterized in that: The protective component includes a cover-applying machine, a support seat is provided above the output end of the cover-applying machine, a second longitudinal cylinder is provided above the support seat, a third carrier plate is provided at the output end of the second longitudinal cylinder, a fifth vertical cylinder is provided on the third carrier plate, a lifting frame is provided at the output end of the fifth vertical cylinder and passes through the third carrier plate, a suction cup with an attraction function is provided below the lifting frame, and a discharge hole is provided in the output end of the cover-applying machine close to the support seat.

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