A fine-tuning type modeling device for spinal cord injury animal models

Abstract

The present invention discloses a fine-tuning type modeling device for spinal cord injury animal models, which mainly includes a clamp body driven by a lead screw transmission mechanism and its opening / closing state indication system. By rotating the adjusting wheel on the adjusting screw of the lead screw transmission mechanism, the insulating chucks at the lower parts of the two opening / closing plates of the clamp body are clamped and opened. During the opening / closing movement of the clamp body, the contact state between the insulating chuck and the spinal cord can be determined through the detection circuit composed of the metal sheet on the insulating chuck and the indicator light. The present invention has the characteristics of low cost, simple operation, and fine quantitative measurement, and is suitable for making spinal cord injury animal models with stable states.

Description

Technical Field

[0001] The present invention relates to a clamping spinal cord injury modeling tool for making a small animal model of spinal cord injury in basic and clinical medical animal experiments, and particularly relates to a fine-tuning type spinal cord injury animal model modeling device. Background Art

[0002] Spinal cord injury (SCI) often occurs due to various accidents, which can cause disabilities such as paraplegia, and has a high disability rate. The sensory, motor, and autonomic nerve functions below the damaged segment of the patient are absent, seriously affecting people's life and work and causing a huge social and economic burden. The repair and rehabilitation treatment after spinal cord injury have always been difficult points that modern medicine needs to overcome. Among them, acute spinal cord injury has always been a hot spot in basic research in the field of spinal surgery. Since the pathophysiological mechanism after spinal cord injury is very complex, it is very important to establish a model through experimental animals.

[0003] Allen pioneered the standardized experimental research on spinal cord injury in 1911 by using a heavy object to fall and impact the dorsal side of the spinal cord. The method of establishing the model requires incising the midline of the animal's back, removing the lamina, and exposing the spinal cord dura mater. Then, an object of a certain weight is freely dropped from a certain height and strikes the spinal cord, thereby causing spinal cord injury. Because most spinal cord injuries in clinical practice are caused by spinal fracture and dislocation, this method cannot fully simulate spinal cord injury in clinical practice. Moreover, spinal cord injury often involves the anterior part of the spinal cord, causing great damage to the anterior spinal artery and relatively less damage to the posterior spinal artery. At the same time, the degree of spinal cord injury caused by impact is related to tissue characteristics such as the diameter, texture, deformation, and displacement degree of the spinal cord. These characteristics of the spinal cord tissue itself are greatly affected by individual differences in animals, lacking personalized intervention standards. The ventral spinal cord injury model designed by Guo Bangfu et al. in 1984 supplemented the Allen dorsal injury modeling method, and the obtained animal model is similar to spinal cord injury in clinical practice. However, due to the lack of impact force, high surgical operation difficulty, and high requirements for equipment, the widespread use of this model is limited. The clamping spinal cord injury animal modeling was first proposed and used by Rivlin in 1978. Specifically, an aneurysm clip is used to cause spinal cord injury. It is necessary to perform a laminectomy on the target segment of the spine first, and then the aneurysm clip is used to close and clamp around the spinal cord with a specific force, which can produce different degrees of acute injury, thereby replicating the common continuous spinal cord compression in clinical practice, and using the obtained model to prove the relationship between the severity of nerve injury and the severity and duration of compression.

[0004] Clamping spinal cord injury is more similar to the pathology and physiology of clinical acute spinal cord injury, suitable for the research of spinal nerve regeneration and related therapeutic experiments. At the same time, the operation is simpler and the cost of model production is lower, so it is widely used at present. However, due to reasons such as individual differences in animals, inconsistent clamping force and clamping angle, and difficulty in controlling the proportion of spinal cord clamping compression, the animal model lacks stability, that is, the degree of spinal cord injury in animals after treatment varies significantly.

[0005] The main reason for the difficulty in quantifying the clamping force during the animal modeling process of clamping spinal cord injury is that the clamping instrument used has a rotary opening and closing mode (similar to the clamping process of tweezers, pliers or vascular clamps). During the process of clamping the spinal cord, the force direction of the spinal cord cannot be ensured to be radial to the spinal cord, and the force magnitude and the rotary opening and closing angle of the clamping instrument are not linearly related, making it difficult to determine the actual clamping force. For this reason, in Chinese Patent CN110584821A, a relative translational opening and closing method of two clamping pliers is adopted, and the clamping force is easier to accurately control, but there are still the following problems: 1) Using pressure as a signal, it is impossible to accurately determine the proportion of spinal cord injury caused by spinal cord clamping compression; 2) Controlling the force using the pressure signal method requires a higher cost, and the control accuracy is affected by the measurement reliability of the equipment. In the face of the differences in the spinal cord sizes of different animal individuals, in order to make the proportion of spinal cord injury close, it is usually achieved by controlling the animal weight, but it is not accurate. Summary of the Invention

[0006] In order to solve the problem in the prior art that it is difficult to create a model with a uniform degree of spinal cord injury for different individuals with anatomical differences, the present invention provides a fine-tuning type spinal cord injury animal model modeling device to provide a spinal cord injury animal model with a quantifiable degree of spinal cord injury and more stable results.

[0007] To achieve the above object, the present invention adopts the following technical solutions:

[0008] A fine-tuning type spinal cord injury animal model modeling device, the modeling device includes a clamping body, a clamping opening and closing adjusting device, and an opening and closing state indicating device; the clamping opening and closing adjusting device includes a fixing plate and a lead screw transmission mechanism arranged on the fixing plate; the clamping body includes a first opening and closing plate and a second opening and closing plate (constituting a pair of opening and closing plates) connected to the lead screw transmission mechanism, and the distance between the first opening and closing plate and the second opening and closing plate can be adjusted in a translational manner through the lead screw transmission mechanism, and each opening and closing plate is connected to a corresponding insulating chuck; the opening and closing state indicating device includes a detection circuit that is turned on as a loop by contacting the spinal cord, and the detection circuit includes conductive contacts arranged on the insulating chucks corresponding to the first opening and closing plate and the second opening and closing plate respectively, and a light source device connected in series with each conductive contact through a wire.

[0009] Preferably, the lead screw transmission mechanism includes an upper slide bar and a lower slide bar serving as guide rails, and a rotatable adjusting screw and an adjusting wheel serving as rotating components. The upper slide bar and the lower slide bar are parallel to each other and are spaced apart on the fixed plate. The adjusting wheel is arranged between the upper slide bar and the lower slide bar through the adjusting screw. The two opening and closing plates (the first opening and closing plate and the second opening and closing plate) of the clamp body are respectively connected to the adjusting screw through adjusting nuts arranged outside the two end faces of the adjusting wheel. The upper slide bar, the lower slide bar, and the adjusting screw respectively penetrate through the two opening and closing plates (the first opening and closing plate and the second opening and closing plate) of the clamp body.

[0010] Preferably, the rotation directions of the two adjusting nuts outside the two end faces of the adjusting wheel are opposite. When the adjusting wheel that can rotate coaxially and at the same speed as the adjusting screw drives the adjusting screw to rotate circumferentially, the two opening and closing plates (the first opening and closing plate and the second opening and closing plate) of the clamp body can perform opening and closing movements along the axial directions of the upper and lower slide bars, thereby changing the distance between the two opening and closing plates.

[0011] Preferably, the clamp opening and closing adjusting device includes two relatively arranged fixed plates. The two ends of the upper slide bar, the lower slide bar, and the adjusting screw are respectively connected to the corresponding sides (inner sides) of the two fixed plates.

[0012] Preferably, the conductive contact piece is a metal layer arranged at the lower part of the insulating chuck, and the light source device is an LED indicator lamp with a power supply. When the two opening and closing plates (the first opening and closing plate and the second opening and closing plate) of the clamp body drive the corresponding insulating chucks to translate towards each other and make the corresponding two conductive contact pieces contact each other (the insulating chucks are clamped), or make the corresponding two conductive contact pieces contact a conductive body such as the spinal cord, the detection circuit forms a loop and the LED indicator lamp lights up, thereby assisting the naked eye to judge the contact state between the spinal cord and the pliers.

[0013] Preferably, the modeling device further includes a fixing device. The fixing device includes a lifting and fixing bracket connected to the fixed plate of the clamp opening and closing adjusting device. The lifting and fixing bracket can adjust the clamp opening and closing adjusting device and the clamp body to an appropriate height by moving up and down, so as to facilitate the clamping and pressing of the animal spinal cord (by controlling the insulating chucks on the two opening and closing plates to squeeze the spinal cord with a certain clamping force).

[0014] Preferably, the fixing device further includes a base, and the lifting and fixing bracket is arranged on the base.

[0015] The calibration method of the above fine-tuning type spinal cord injury animal model modeling device includes the following steps:

[0016] 1) Make the first opening and closing plate and the second opening and closing plate drive the corresponding insulating chucks to translate towards each other, and make the conductive contact pieces on the corresponding insulating chucks contact each other. When the light source device in the detection circuit is powered on, record the distance between the first opening and closing plate and the second opening and closing plate and use it as X0;

[0017] 2) Place the animal spinal cord between the first opening and closing plate and the second opening and closing plate, then move the first opening and closing plate and the second opening and closing plate driven by the corresponding insulating chucks towards each other, and make the corresponding two conductive contacts (10) move towards the animal spinal cord. When the light source device in the detection circuit is powered on, record the distance between the first opening and closing plate and the second opening and closing plate as X1.

[0018] Preferably, the calibration method further includes the following steps:

[0019] 3) Determine the moving distances of the first opening and closing plate and the second opening and closing plate corresponding to the target spinal cord compression ratio according to X0 and X1.

[0020] Preferably, the clamping force of the clamping body during the process of compressing the animal spinal cord is determined by controlling the moving distance of the first opening and closing plate and the second opening and closing plate driven by the lead screw transmission mechanism and / or the rotation time of the rotating component of the lead screw transmission mechanism.

[0021] The beneficial effects of the present invention are reflected in:

[0022] The present invention uses a lead screw transmission mechanism to change the clamping body from a rotary opening and closing mode to a translational opening and closing mode, which can not only give a constant clamping force and clamping angle to the spinal cord, but also adjust the proportion of the animal spinal cord damaged by compression through the moving distance of the opening and closing plate, so as to cause quantitative damage to the animal spinal cord, and conveniently realize the modeling process of spinal cord injury animal models with different injury degrees. For different animal species or different individuals of the same species, quantitative adjustment can be carried out at the individual level of experimental animals, so as to ensure the same injury degree of the spinal cord model and improve the stability of building spinal cord injury models using different individuals with anatomical differences. At the same time, the present invention can conveniently and quickly determine the states when the insulating chuck is completely clamped and just touches the spinal cord through the detection circuit, thus providing a reference point for quantitatively adjusting the proportion of spinal cord injury caused by compression, and can indirectly calculate the spinal cord diameter. The present invention has the characteristics of delicate structure, simple operation, low modeling cost, and fine quantitative model, and can be used to make spinal cord injury animal models with stable states.

[0023] Furthermore, the clamping body of the present invention can be perpendicular to the horizontal plane, the force direction of the spinal cord during clamping is radially inward along the spinal cord, the force magnitude is realized by rotating the adjusting wheel, and the proportion of spinal cord injury caused by compression is proportional to the translational distance of the opening and closing plate of the clamping body.

[0024] Furthermore, in the present invention, the clamping force can be quantitatively calculated according to the lead screw movement principle, and the effective quantitative control of the clamping force during the spinal cord compression process can be realized by adjusting the rotation time of the adjusting wheel and / or the moving distance of the opening and closing plate. Description of the Drawings

[0025] Figure 1Schematic diagram of the structure of a fine-tuning spinal cord injury animal model maker;

[0026] Figure 2 Schematic diagram of the zeroing state of the model maker;

[0027] Figure 3 Schematic diagram of the state of the model maker clamping the spinal cord;

[0028] Figure 4 Schematic diagram of the process of making a spinal cord injury model by clamping injury with the model maker;

[0029] In the figure: lifting and fixing bracket 1, fixing plate 2, upper sliding rod 3, lower sliding rod 4, adjusting screw 5, adjusting wheel 6, adjusting nut 7, opening and closing plate 8, insulating chuck 9, conductive contact 10, indicator light 11, counterweight base 12. Specific implementation manners

[0030] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments. The embodiments are only used to explain the present invention and do not limit the protection scope of the present invention.

[0031] (1) Structure and control principle of a fine-tuning spinal cord injury animal model maker

[0032] See Figure 1, the fine-tuning spinal cord injury animal model making device includes a clamping body, a clamping opening / closing adjusting device, a fixing device, and an opening / closing state indicating device. The clamping body includes two opening / closing plates 8. The clamping opening / closing adjusting device includes two left and right fixing plates 2 that are perpendicular to the horizontal plane and arranged oppositely. Between the two fixing plates 2, upper sliding rods 3, adjusting screws 5, and lower sliding rods 4 of equal length and arranged in parallel are fixed from top to bottom. The two ends of the upper and lower sliding rods 3 and 4 are fixed to the corresponding fixing plates 2 at the corresponding ends and cannot rotate circumferentially (circumferentially fixed), and the two ends of the adjusting screw 5 are movably connected to the corresponding fixing plates 2 (not circumferentially fixed) and can rotate circumferentially; the two opening / closing plates 8 of the clamping body vertically pass through the three parallel rods (the upper and lower sliding rods 3, 4, and the adjusting screw 5), and are symmetrically placed on the left and right along the adjusting wheel 6 fixed at the midpoint position of the adjusting screw 5. The two opening / closing plates 8 are connected to the adjusting screw 5 through an adjusting nut 7 fixed on each opening / closing plate 8 (the nut is embedded in the adjusting screw through hole on the opening / closing plate), and the two adjusting nuts 7 are screwed in opposite directions (corresponding to the threaded sections of the adjusting screw 5 on both sides of the adjusting wheel 6). By using the lead screw transmission mechanism formed by the adjusting screw 5, the upper and lower sliding rods 3, 4, and the adjusting nuts 7 on the opening / closing plates 8, when the adjusting wheel 6 is rotated to drive the coaxial rotation of the adjusting screw 5, the two opening / closing plates 8 of the clamping body can make an opening / closing movement towards or away from each other along the axial direction of the adjusting screw 5 under the guidance of the upper and lower sliding rods 3 and 4, and by reducing the speed of the lead screw transmission, the fine-tuning of the distance between the two opening / closing plates 8 can be realized. On the relatively inner sides of the lower parts of the two opening / closing plates 8 of the clamping body, an insulating chuck 9 is respectively installed. The insulating chuck 9 is processed from the tip of a pair of tweezers, with the tip facing downwards. Twice the thickness of the root (upper end) of the insulating chuck 9 should be greater than the thickness of the adjusting wheel 6 to ensure that the adjusting wheel 6 can still rotate when the two tweezers tips are completely closed. Thus, through the process of rotating the adjusting wheel 6, the fine adjustment of the opening / closing state of the clamping body is realized.

[0033] The fixing device includes a counterweight base 12 placed on the test bench and a lifting and fixing bracket 1. The lifting and fixing bracket 1 is respectively connected to the counterweight base 12 and one of the two fixing plates 2 located above the right side of the counterweight base 12. Through the lifting and fixing bracket 1, the distance between the main body of the model making device (i.e., the clamping opening / closing adjusting device and the clamping body) and the counterweight base 12 below it can be adjusted. The counterweight plate installed in the counterweight base 12 extends towards the left side of the counterweight base 12 to ensure the stability of the main body of the model making device.

[0034] The opening and closing state indicating device includes a detection circuit formed by connecting a conductive contact piece 10, a wire, and a low-power LED indicator light 11 with a power supply in series. Among them, there are two conductive contact pieces 10, both made of ultra-thin metal sheets (for example, a coating with a thickness of about 1 / 100 mm). To prevent the bone on both sides of the spinal canal from contacting the insulating chuck 9 to form a circuit and causing the indicator light 11 to turn from off to on, the conductive contact pieces 10 are embedded in the form of ultra-thin metal sheets only on the opposite side (inner side) of the lower part of the two insulating chucks 9. Therefore, when the two insulating chucks 9 are short-circuited or only the inner side contacts the spinal cord, the indicator light turns from off to on (the conductive contact piece is on one side of the spinal cord, and the bone side contacts the insulating chuck without forming a circuit).

[0035] See Figure 2 , rotate the adjustment wheel 6. When the two insulating chucks 9 are completely in contact, the detection circuit is short-circuited to form a circuit. When the indicator light 11 just lights up, the distance between the two opening and closing plates 8 is recorded as 0 or 0% (the position of the opening and closing plates 8 can be marked on the rod body of the upper sliding rod 3 or the lower sliding rod 4 with a fine marker), corresponding to the two insulating chucks 9 being completely clamped shut.

[0036] See Figure 3 and Figure 4 , when using the insulating chuck 9 to clamp the exposed spinal cord of an experimental animal, rotate the adjustment wheel 6. The two conductive contact pieces 10 move towards the spinal cord. When they just contact the outer edges of both sides of the spinal cord, the detection circuit forms a circuit, causing the indicator light 11 to light up. At this time, the distance between the two opening and closing plates 8 is recorded as 1 or 100% (the position of the opening and closing plates 8 can be marked on the rod body of the upper sliding rod 3 or the lower sliding rod 4 with a fine marker), corresponding to the insulating chuck 9 just contacting the spinal cord. Further rotating the adjustment wheel 6 will cause the spinal cord to be compressed and injured by the insulating chuck 9. When the two opening and closing plates 8 move to reach the target compression state, the positions of the two opening and closing plates 8 can be confirmed by marking them on the rod body of the upper sliding rod 3 or the lower sliding rod 4 with a fine marker. That is, the injury degree of the spinal cord injury model caused by clamping can be selected according to the percentage value between 0% and 100%. During the process of compressing the spinal cord, the compression time is determined according to the time from when the indicator light 11 starts to light up to when it goes out. That is, the clamping time starts when the indicator light 11 lights up as the conductive contact piece 10 in the detection circuit contacts the spinal cord to form a circuit, and ends when the circuit formed by the detection circuit is disconnected (the conductive contact piece 10 no longer contacts the spinal cord) and the indicator light goes out.

[0037] (2) Modeling examples of the fine-tuning type spinal cord injury animal model making device

[0038] Taking rats as an example, the specific experimental process operation steps are as follows:

[0039] Fifteen rats weighing 180 - 220 g were selected, fasted and water - deprived for 12 hours. Before the operation, they were anesthetized by intraperitoneal injection of 10% chloral hydrate (4 ml / kg) and the back was shaved to prepare a sterile environment for the operation. The four limbs of the rats were fixed to the four feet of the animal fixing plate. In the prone position, the back skin and muscles were successively cut open to expose the spinous process. The paravertebral muscles and the spine were separated through a micro - spinal retractor. The 10th thoracic vertebra was selected, and the bilateral laminae were cut off in total. The superior and inferior articular processes, spinous process and laminae of the vertebra were completely removed. At this time, most of the spinal cord was exposed. The head of the forceps was disassembled and disinfected by ultraviolet irradiation.

[0040] Completely close the insulating collet 9. When the indicator light 11 just lights up, measure the distance between the two opening - closing plates 8 with a vernier caliper, and record this distance as X0.

[0041] Dry the liquid around the exposed spinal cord. By adjusting the height and orientation of the lifting and fixing bracket 1, make the insulating collet 9 just stuck in the two - side gaps between the spinal canal and the spinal cord, align the center of the spinal cord with the marked X0 position. Slightly rotate the adjusting wheel 6. When the indicator light 11 just starts to light, measure the distance between the two opening - closing plates 8, and record this distance as X1 (the spinal cord diameter is X1 - X0). The movable distance range of the opening - closing plate 8 is between X0 and X1. After that, if the target compression ratio reaches a% of the spinal cord diameter, then when moving to the end point, the distance X between the two opening - closing plates 8 a The calculation formula is:

[0042] X a = a%×(X1 - X0)+X0

[0043] And the moving distance l of the unilateral opening - closing plate 8 is Xa / 2.

[0044] Rotate the adjusting wheel 6 to make the two opening - closing plates 8 reach a distance of X a position. After timing for 30 seconds, adjust the two opening - closing plates 8 to a distance of X1 position, and remove the modeling device. Then suture the muscles and skin in turn and disinfect. After the anesthetized rats wake up, implement nursing care, and relevant experimental interventions or morphological and behavioral evaluations of the degree of spinal cord injury can be carried out. The force during the compression process is proportional to the torque T of rotating the adjusting wheel 6:

[0045] T = J·β:

[0046] Among them, J is the inertia of the rotating parts (adjusting wheel and adjusting screw) during the rotation process, J=(mass of the adjusting wheel + mass of the adjusting screw)×square of the radius of the adjusting wheel; β is the average angular acceleration of the rotation. According to the relationship between the lead - screw transmission distance and the rotation radian, within t seconds, the moving distance of the unilateral opening - closing plate is l, and the rotated radian is 2πl / L (where L is the lead of the adjusting screw, that is, the distance that the spiral advances when rotating 360°). According to the angular - acceleration - time and rotation - radian relationship equation can be solved to obtain

[0047] The relationship between the torque T and the output force P theoretically follows the formula for the output force of a lead screw motion:

[0048]

[0049] Among them, the total mass m of the rotating components, the radius r of the adjusting wheel, the friction coefficient η, and the lead L of the adjusting screw are all constant values. By controlling the opening and closing distance (determined by the above l) and the rotation time (i.e., the above t), the clamping force can be indirectly determined.

[0050] According to the operation of this example, when the spinal cord was clamped to 20% of its original level, all the rats showed typical hindlimb dysfunction and could not urinate autonomously after the operation, and artificial assisted urination was required. The wound healed well after the operation. One week after the operation, the rats recovered the function of autonomous urination.

[0051] In summary, the above-mentioned fine-tuning type spinal cord injury animal model maker has a simple structure, is easy to quantitatively control, has stable modeling results, has no additional adverse effects on animals, and can be used in the research of clinical basic medicine for spinal cord injury with high requirements for model stability.

Claims

1. A spinal cord injury animal model making device, characterized in that: The modeling device includes a clamping body, a clamping opening / closing adjusting device, and an opening / closing state indicating device; the clamping opening / closing adjusting device includes a fixing plate (2) and a lead screw transmission mechanism arranged on the fixing plate (2); the clamping body includes a first opening / closing plate and a second opening / closing plate with adjustable spacing connected to the lead screw transmission mechanism, and insulating chucks (9) for pressing the animal spinal cord and adjusting the proportion of the damaged animal spinal cord according to the moving distance of the opening / closing plates are respectively connected to the first opening / closing plate and the second opening / closing plate; the opening / closing state indicating device includes a detection circuit, and the detection circuit includes conductive contacts (10) arranged on the insulating chucks (9) respectively connected to the first opening / closing plate and the second opening / closing plate and a light source device connected to each conductive contact (10); The lead screw transmission mechanism includes a guide rail for the first opening / closing plate and the second opening / closing plate to translate, a rotatable adjusting screw (5), and an adjusting wheel (6). The adjusting wheel (6) is connected to the adjusting screw (5). The first opening / closing plate and the second opening / closing plate are respectively connected to the adjusting screw (5) through adjusting nuts (7) arranged outside the two end faces of the adjusting wheel (6), and the screwing directions of the adjusting nuts (7) outside the two end faces of the adjusting wheel (6) are opposite; Determine the clamping force of the clamping body during the process of compressing the animal's spinal cord by controlling the moving distance of the first opening and closing plate and the second opening and closing plate driven by the lead screw drive mechanism and the rotation time of the rotating component of the lead screw drive mechanism. And when the moving distance of the unilateral opening and closing plate within t seconds is l i.e., Xa / 2, determine the magnitude of the clamping force according to the following formula, that is, the output force P : P = Among them, m is the total mass of the rotating components, namely the adjusting wheel and the adjusting screw, r is the radius of the adjusting wheel, η is the friction coefficient, L is the lead of the adjusting screw; X a = a% × (X1 - X0) + X0 a% is the target compression ratio of the clamped spinal cord, X1 - X0 is the spinal cord diameter, and X0 is the distance between the two opening / closing plates when the insulating chuck is completely clamped.

2. The spinal cord injury animal model making device according to claim 1, wherein: The guide rail includes an upper sliding rod (3) and a lower sliding rod (4). The upper sliding rod (3) and the lower sliding rod (4) are parallel to each other and are spaced apart on the fixing plate (2). The adjusting wheel (6) is arranged between the upper sliding rod (3) and the lower sliding rod (4) through the adjusting screw (5). The upper sliding rod (3), the lower sliding rod (4), and the adjusting screw (5) penetrate through the first opening / closing plate and the second opening / closing plate.

3. The spinal cord injury animal model making device according to claim 2, characterized in that: Both ends of the upper sliding rod (3), the lower sliding rod (4), and the adjusting screw (5) are respectively connected to the fixing plate (2).

4. The spinal cord injury animal model making device according to claim 1, wherein: The conductive contact (10) is a metal layer arranged at the lower part of the insulating chuck (9), and the light source device is an LED indicator light (11) with a power supply.

5. The spinal cord injury animal model making device according to claim 1, characterized in that: The modeling device further includes a fixing device, and the fixing device includes a lifting and fixing bracket (1) connected to the fixing plate (2).

6. The spinal cord injury animal model making device according to claim 5, characterized in that: The fixing device further includes a base, and the lifting and fixing bracket (1) is arranged on the base.

7. A calibration method for a spinal cord injury animal model making device as described in claim 1, characterized in that: This calibration method includes the following steps: 1) Make the first opening / closing plate and the second opening / closing plate drive the corresponding insulating chucks (9) to translate towards each other, and make the conductive contacts (10) on the corresponding insulating chucks (9) contact each other. When the light source device in the detection circuit is powered on, record the distance between the first opening / closing plate and the second opening / closing plate and use it as X0, X0 = 0 or 0%; 2) After placing the animal spinal cord between the first opening / closing plate and the second opening / closing plate, make the first opening / closing plate and the second opening / closing plate drive the corresponding insulating chucks (9) to translate towards each other, and make the corresponding two conductive contacts (10) move towards the animal spinal cord. When the light source device in the detection circuit is powered on, record the distance between the first opening / closing plate and the second opening / closing plate and use it as X1, X1 = 1 or 100%; The animal model of spinal cord injury is a clamped spinal cord injury model, and the degree of spinal cord injury is selected according to the value between X0 and X1.

8. The calibration method according to claim 7, wherein: The calibration method further includes the following steps: 3) Determine the moving distances of the first opening and closing plate and the second opening and closing plate corresponding to the target spinal cord compression ratio according to X0 and X1.

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