Sample precision controller and its usage method

Abstract

The present invention belongs to the field of egg cell operation instruments, and discloses a sample precision controller and a using method thereof. Among them, the sample precision controller includes a main machine chamber, a handheld part and a connecting pipe. The main machine chamber is equipped with a display and a driving mechanism. The driving mechanism is provided with a connection port. The handheld part includes a gripping part, and a through connection channel is arranged on the gripping part. One end of the connecting pipe is communicated with the end of the connection channel, and the other end of the connecting pipe is communicated with the connection port. The driving mechanism is separated from the handheld part, which improves the control precision during the egg stripping operation.

Description

Technical Field

[0001] The invention belongs to the field of egg cell and embryo operation instruments, in particular to a sample precision control instrument and a use method thereof. Background Art

[0002] "Test tube baby" is a baby that is born by artificially fertilizing eggs and sperm in vitro, undergoing early embryonic development, and then transplanted into the mother's uterus for development. During the entire in vitro fertilization and cultivation process, egg peeling and sample transfer are important parts of the daily work of assisted reproductive laboratories. Egg peeling is the process of removing the granulosa cells surrounding the egg to expose the egg. It is mainly used to assess the maturity of the oocyte, assess the fertilization of the egg, facilitate egg freezing and facilitate micromanipulation. The operational details during egg peeling directly affect the results of subsequent operational links; sample transfer involves the transfer of various types of sperm, eggs, and fertilized eggs from one type of culture medium to other culture media, and also has high requirements for the stability and accuracy of its operation.

[0003] Among the existing egg stripping tools, for example, a Chinese patent with application number 201310034724.3 discloses a novel in vitro fertilization egg stripping operator, which is operated by hand and forms a pressure difference by squeezing a soft capsule to absorb liquid. However, this method relies on manual pressing each time, and the suction force cannot be controlled. In addition, the hand force during the pressing process is prone to shaking, so it is easy to fail to complete the suction at the same time during the operation, resulting in a significant reduction in control accuracy and affecting the operation efficiency. Summary of the invention

[0004] The purpose of the present invention is to improve the shortcomings of the prior art and provide a sample precision controller and a method for using the same to improve the control accuracy during egg stripping operations and sample transfer.

[0005] The technical solution is as follows:

[0006] The sample precision controller includes a main chamber, a handpiece and a connecting tube. The main chamber is equipped with a display and a driving mechanism. The driving mechanism is provided with a connecting port. The handpiece includes a gripping portion. The gripping portion is provided with a through connecting channel. One end of the connecting tube is connected to the end of the connecting channel, and the other end of the connecting tube is connected to the connecting port.

[0007] In one embodiment, the driving mechanism includes a power member and a plunger member, the connecting port is provided at the bottom of the plunger member, and the power member drives the plunger member to move.

[0008] In one embodiment, the drive mechanism further includes a transmission member, the power member is a linear motion motor, the plunger member includes a plunger rod and a plunger sleeve, the plunger rod is matched with the plunger sleeve, a connection port is opened at the bottom of the plunger sleeve, one end of the transmission member is connected to the output end of the linear motion motor, and the other end is connected to the plunger rod.

[0009] In one embodiment, the transmission member includes a guide block and a transmission block. The guide block has a first end and a second end, the first end and the second end form a right angle, the first end is slidably matched with the outer shell of the linear motion motor, the second end is connected to the output end of the linear motion motor, the transmission block is fixedly connected to the second end of the guide block, and the transmission block is simultaneously connected to one end of the plunger rod.

[0010] In one embodiment, an adapter is further installed in the main chamber, the adapter is connected to the lower end of the plunger sleeve, and the connection port is located within the adapter, and the connecting pipe is communicated with the adapter.

[0011] In one embodiment, an oviduct stripping tube is further connected to the connection channel of the grasping portion.

[0012] In one embodiment, a receiving member is provided on the outside of the main chamber, both side portions of the receiving member protrude, and the hand-held member can be placed between the protrusions of the receiving member.

[0013] In one embodiment, the drive mechanism, the connecting pipe and the hand-held member are injected with liquid.

[0014] In one embodiment, a foot pedal interface is further provided in the main chamber.

[0015] The usage method of the sample precision controller includes the following steps:

[0016] Install the oviduct stripping tube on the grasping portion;

[0017] Turn on the power of the main chamber, and the display shows and starts the program;

[0018] Grasp the hand-held member, move it to the pre-use position, start the drive mechanism to generate a pressure difference, the connecting pipe is communicated with the connection port, and under the conduction of the connecting pipe, the pressure difference is conducted to the connection channel of the grasping portion and then continues to be conducted to the oviduct stripping tube;

[0019] Due to the pressure difference, the oviduct stripping tube can suck and spray liquid or liquid containing egg cells.

[0020] The technical solution provided by the present invention has the following advantages and effects:

[0021] 1. After assembly, start the main chamber. The display in the main chamber will show the started program, and then grasp the handheld part, which is installed with an egg extraction tube at this time. When the main chamber is started, the driving mechanism is started to generate a pressure difference, and then it is conducted to the connection channel of the grasping part under the action of the connecting pipe. Therefore, the egg extraction tube can suck and spit out liquid and liquid containing egg cells under the action of the pressure difference. During this process, the operation is to pick up the handheld part, and there is no driving mechanism on the handheld part, and its structure is relatively simple, so the handheld part is also lighter and not easy to get tired after long-term grasping. In addition, since the driving mechanism is set on the main chamber, when liquid needs to be sucked and spit out, power is provided by the main chamber, so the vibration sensation will not be conducted to the handheld part, and thus the control accuracy is greatly improved when operating the handheld part.

[0022] 2. The pressure difference generated by the driving mechanism needs to be transmitted through the connecting pipe. If gaseous medium is directly used for transmission, due to the obvious compression effect, it will cause conduction hysteresis, which will amplify the error for each small amount of egg extraction operation and affect the liquid suction accuracy. Here, liquid is injected into the connecting pipe and the driving mechanism, specifically in the plunger sleeve and the connecting channel of the handheld part. The liquid has poor compressibility, so the conduction efficiency is high, and the pressure difference conducted by the driving mechanism can be quickly conducted to the egg extraction tube with low delay and low error. Brief Description of the Drawings

[0023] The drawings here show specific examples of the technical solutions of the present invention and form a part of the specification together with the specific implementation manners, and are used to explain the technical solutions, principles and effects of the present invention.

[0024] Unless otherwise specified or defined, in different drawings, the same reference numerals represent the same or similar technical features, and for the same or similar technical features, different reference numerals may also be used for representation.

[0025] Figure 1 is a three-dimensional structural schematic diagram of an embodiment of the present invention;

[0026] Figure 2 is a structural schematic diagram of removing the protection plate of an embodiment of the present invention;

[0027] Figure 3 is a structural schematic diagram of the back of an embodiment of the present invention;

[0028] Figure 4 is a structural schematic diagram of the handheld part of an embodiment of the present invention;

[0029] Figure 5 is an exploded structural schematic diagram of an embodiment of the present invention;

[0030] Figure 6 is a cross-sectional structural schematic diagram before the driving mechanism operates in an embodiment of the present invention;

[0031] Figure 7 It is a schematic cross-sectional structure diagram when the driving mechanism of the embodiment of the present invention operates;

[0032] Figure 8 It is a schematic three-dimensional structure diagram of the cooperation between the operation of the driving mechanism and the adapter of the embodiment of the present invention;

[0033] Figure 9 It is a schematic cross-sectional structure diagram of the cooperation between the operation of the driving mechanism and the adapter of the embodiment of the present invention.

[0034] Explanation of reference numerals:

[0035] 10. Main engine room; 11. Display; 12. Driving mechanism; 121. Power component; 1211. Linear motion motor; 122. Plunger component; 1221. Plunger rod; 1222. Plunger sleeve; 123. Transmission component; 1231. Guide block; 1232. Transmission block; 13. Adapter; 131. Adapter port; 14. Placing component; 15. Foot pedal interface; 16. Position detector; 17. Control button; 18. Support component; 20. Handheld component; 21. Gripping part; 30. Connecting pipe. Detailed implementation manners

[0036] For the convenience of understanding the present invention, the specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings of the specification.

[0037] Unless otherwise specified or defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the technical field to which the present invention belongs. In the case of combining the technical solutions of the present invention with a realistic scenario, all technical and scientific terms used herein may also have meanings corresponding to the purpose of implementing the technical solutions of the present invention.

[0038] Unless otherwise specified or defined, the "first, second..." used herein is only for distinguishing names and does not represent a specific quantity or order.

[0039] Unless otherwise specified or defined, the term "and / or" used herein includes any and all combinations of one or more related listed items.

[0040] It should be noted that when an element is considered to be "fixed to" another element, it can be directly fixed to the other element or there can be an intermediate element; when an element is considered to be "connected to" another element, it can be directly connected to the other element or there can be an intermediate element at the same time; when an element is considered to be "mounted on" another element, it can be directly mounted on the other element or there can be an intermediate element at the same time. When an element is considered to be "provided in" another element, it can be directly provided in the other element or there can be an intermediate element at the same time.

[0041] In this solution, it can be used for the operation of egg cells and embryos. Specifically, in this embodiment, the operation of egg cells is taken as an example.

[0042] As Figures 1 to 6 shown, the sample precision controller includes a main chamber 10, a handheld part 20 and a connecting pipe 30. A display 11 and a driving mechanism 12 are installed in the main chamber 10. The driving mechanism 12 is provided with a connection port. The handheld part 20 includes a gripping part 21. A through connection channel is provided on the gripping part 21. One end of the connecting pipe 30 is communicated with the end of the connection channel, and the other end of the connecting pipe 30 is communicated with the connection port. An ovum stripping pipe is also connected to the connection channel of the gripping part 21.

[0043] After assembly is completed, start the main chamber 10. The display 11 of the main chamber 10 will display the started program. Then grip the handheld part 20. At this time, the handheld part 20 is installed with an ovum stripping pipe. The main chamber 10 is started, and the driving mechanism 12 is started to generate a pressure difference, which is then conducted to the connection channel of the gripping part 21 under the action of the connecting pipe 30. Therefore, the ovum stripping pipe can suck and spit out liquid and liquid containing egg cells under the action of the pressure difference. During this process, the operation is to pick up the handheld part 20, and there is no driving mechanism 12 on the handheld part 20, and its structure is relatively simple, so the handheld part 20 is also lighter and not easy to get tired after being gripped for a long time. In addition, since the driving mechanism 12 is provided on the main chamber 10, when liquid needs to be sucked and spit out, power is provided by the main chamber 10, so the vibration feeling will not be conducted to the handheld part 20. Therefore, the control precision of the handheld part 20 is greatly improved during operation.

[0044] The above is the ovum stripping pipe used in the ovum stripping mode. When transferring samples, a sample transfer pipe is needed, and its space is larger than that of the ovum stripping pipe. The large space of the sample transfer pipe can be suitable for embryo transfer.

[0045] As Figures 5 to 7 shown, the driving mechanism 12 includes a power member 121 and a plunger member 122. The connection port is provided at the bottom of the plunger member 122, and the power member 121 drives the plunger member 122 to move. The pressure difference generated by the movement of the plunger member 122 is conducted to the handheld part 20 through the connecting pipe 30.

[0046] Specifically, the driving mechanism 12 further includes a transmission member 123, the power member 121 is a linear motion motor 1211, the plunger member 122 includes a plunger rod 1221 and a plunger sleeve 1222, the plunger rod 1221 is matched with the plunger sleeve 1222, a connection port is opened at the bottom of the plunger sleeve 1222, one end of the transmission member 123 is connected to the output end of the linear motion motor 1211, and the other end is connected to the plunger rod 1221. The linear motion motor 1211 makes a linear movement, and then drives the plunger rod 1221 to move up and down under the action of the transmission member 123. The plunger rod 1221 is in sliding fit with the plunger sleeve 1222, and the pressure difference generated by the up and down movement of the plunger rod 1221 is conducted to the connecting pipe 30 through the connection port.

[0047] As Figure 1 and Figure 2 shown, the plunger member 122 is provided with a spare part, which is installed on the main engine room 10 and blocked by a protection plate. When the plunger member 122 needs to be replaced, the protection plate is removed to replace the plunger member 122.

[0048] As Figure 6 and Figure 7 shown, the transmission member 123 includes a guide block 1231 and a transmission block 1232. The guide block 1231 has a first end and a second end, the first end and the second end are at a right angle, the first end is in sliding fit with the outer shell of the linear motion motor 1211, the second end is connected to the output end of the linear motion motor 1211, the transmission block 1232 is fixedly connected to the second end of the guide block 1231, and the transmission block 1232 is simultaneously connected to one end of the plunger rod 1221. Since the output end of the linear motion motor 1211 cannot provide lateral force, and in order to protect the output end from being damaged by lateral force, the guide block 1231 is provided. The guide block 1231 is in sliding fit with the outer shell of the linear motion motor 1211, and the second end is used for force at this time. Then, the plunger rod 1221 is connected by the transmission block 1232 to complete the movement of the linear motion motor 1211 driving the plunger rod 1221, which can ensure the sliding precision problem. In the case of egg extraction with high-precision requirements, the precision can be improved, and at the same time, the linear motion motor 1211 and the plunger member 122 can be protected from being damaged by lateral force.

[0049] As Figure 8 and Figure 9As shown, a transfer member 13 is also installed in the main engine room 10. The transfer member 13 is connected to the lower end of the plunger sleeve 1222, and the connection port is located inside the transfer member 13. The connecting pipe 30 is communicated with the transfer member 13. The transfer member 13 forms a connecting medium between the plunger sleeve 1222 and the connecting pipe 30, and the transfer member 13 plays a role in fixing the plunger sleeve 1222. Two transfer ports 131 are provided on the transfer member 13. One of the transfer ports 131 is used to communicate with the connecting pipe 30, and the other transfer port 131 is blocked. The first transfer port 131 communicates with the second transfer port 131.

[0050] The driving mechanism 12, the connecting pipe 30, and the handheld member 20 are filled with liquid. The pressure difference generated by the driving mechanism 12 needs to be transmitted through the connecting pipe 30. If gaseous medium is directly used for transmission, due to the obvious compression effect, there will be a lag in conduction, which will cause the error to be amplified for each small amount of egg stripping operation and affect the liquid suction accuracy. Here, the connecting pipe 30 and the driving mechanism 12, specifically in the plunger sleeve 1222 and the connecting channel of the handheld member 20, are filled with liquid. The liquid has poor compressibility, so the conduction efficiency is high, and the pressure difference conducted by the driving mechanism 12 can be quickly conducted to the egg stripping tube with low delay and low error. Specifically, liquid paraffin oil is used in this embodiment.

[0051] When injecting liquid, it is injected through the second transfer port 131 and blocked after being filled. The replenishment and discharge of liquid can be completed through the second transfer port 131.

[0052] As Figure 1 and Figure 2 shown, a receiving member 14 is provided outside the main engine room 10. The two side parts of the receiving member 14 protrude, and the handheld member 20 can be placed between the protrusions of the receiving member 14. During the operation gap, the handheld member 20 can be placed on the receiving member 14 instead of on the workbench, which may contaminate the handheld member 20 or even accidentally damage the handheld member 20.

[0053] As Figure 3 shown, the main engine room 10 is also provided with a foot pedal interface 15. During the egg stripping operation and the egg transfer operation, the start and stop of the driving mechanism 12 are controlled by foot, avoiding the need to operate the driving mechanism 12 by hand while holding the handheld member 20.

[0054] A power supply connection port is also provided on the back of the main engine room 10, and the foot pedal interface 15 is also provided on the back of the main engine room 10.

[0055] As Figure 4 shown, a support member is provided at the bottom of the main engine room 10, and the support member can be folded. When retracted, the main engine room 10 lies flat, and when unfolded, the main engine room 10 has an inclined angle, which is convenient for viewing the display 11.

[0056] As Figure 1 and Figure 2 shown, operation buttons 17 are provided on the main engine room 10. The operating member is used to select program settings. Of course, the cost can also be increased to set the display 11 as a touch screen for direct touch operation.

[0057] As Figure 8 shown, a position detector 16 is provided inside the main engine room 10, and a baffle is provided on the guiding block 1231. The position detector 16 detects the position where the baffle is located to control the movement limit of the driving linear motion motor 1211.

[0058] The usage method of the sample precision controller includes the following steps: an ovum extraction tube is installed on the grasping part 21; the power supply of the main engine room 10 is turned on, and the display 11 displays and starts the program; the handheld part 20 is grasped and moved to the pre-use position, and the driving mechanism 12 is started to generate a pressure difference. The connecting pipe 30 is communicated with the connection port. Under the conduction of the connecting pipe 30, the pressure difference is conducted to the connection channel of the grasping part 21 and then continues to be conducted to the ovum extraction tube; due to the action of the pressure difference, the ovum extraction tube can suck and spit out liquid or liquid containing oocytes.

[0059] There are various program settings for the sample precision controller, such as the ovum extraction mode and the sample transfer mode.

[0060] The ovum extraction mode is the operation of removing peripheral granulosa cells from the cumulus-oocyte complex. In the ovum extraction mode, the liquid retention volume and the liquid suction and spit volume during operation are set. The liquid retention volume is the amount of liquid pre-aspirated, which can also be understood as the balanced liquid volume. After a new ovum extraction tube is immersed in the liquid, this part of the liquid volume will be aspirated in a set amount to balance the internal and external pressures of the new ovum extraction tube. After the entire ovum extraction operation is completed, this part of the liquid retention volume will remain in the ovum extraction tube and will not be ejected, which can effectively prevent the sample from being adhered or left inside the ovum extraction tube. The liquid suction volume during operation is equal to the liquid spit volume.

[0061] The sample transfer mode is the sample transfer operation. In the sample transfer mode, there are usually two parameters, one is the lower limit of liquid spitting, and the other is the upper limit of liquid suction. The upper limit of liquid suction is the upper limit of the liquid inhalation volume. Setting the upper limit is to prevent the liquid from being inhaled without limit, resulting in liquid contamination or even sample loss. The lower limit of liquid spitting is the lower limit of the liquid reserved after the liquid is spit out, to prevent a large number of bubbles from being blown out during the unlimited liquid spitting operation. It is set according to the size of the sample transfer tube. The commonly set range of the lower limit of liquid spitting is 0 - 99.9 μL, and the set range of the upper limit of liquid suction is 0.1 - 99.9 μL.

[0062] The liquid suction and ejection speed can also be set. The liquid suction and ejection speed has multiple levels, and commonly there are three speeds: low, medium, and high. More speeds can also be set in different usage scenarios. After setting the liquid suction and ejection speed, the speeds in the egg stripping mode and the sample transfer mode are also set accordingly. The speed output is controlled by a foot pedal externally connected to the main engine room 10. The right foot pedal is for setting the selection of the egg stripping mode, and the left foot pedal is default for the low-speed egg stripping mode; in the sample transfer mode, according to the set lower limit of liquid ejection, upper limit of liquid suction, and speed, the right foot pedal is for the suction operation, and the left foot pedal is for the ejection operation.

[0063] Of course, the specific settings are made according to the usage requirements, and only one case is listed here.

[0064] When referring to the drawings for explanation, it is to explain the newly appeared features; in order to avoid the description being not concise enough due to repeated reference to the drawings, for the features that have been described clearly, the drawings are not referenced one by one.

[0065] The purpose of the above embodiments is to exemplarily reproduce and deduce the technical solutions of the present invention, and to completely describe the technical solutions, purposes, and effects of the present invention. The purpose is to make the public understand the disclosed content of the present invention more thoroughly and comprehensively, and it does not limit the protection scope of the present invention.

[0066] The above embodiments are not an exhaustive list based on the present invention. In addition, there may be multiple other embodiments not listed. Any substitution and improvement made on the basis of not violating the concept of the present invention fall within the protection scope of the present invention.

Claims

1. Sample precision controller, characterized in that, It includes a main body chamber, a handheld part, and a connecting pipe. The main body chamber is equipped with a display and a driving mechanism. The driving mechanism is provided with a connection port. The handheld part includes a gripping portion, and a through connection channel is provided on the gripping portion. One end of the connecting pipe is communicated with the end of the connection channel, and the other end of the connecting pipe is communicated with the connection port; The driving mechanism includes a power member and a plunger member. The connection port is provided at the bottom of the plunger member, and the power member drives the plunger member to move; The driving mechanism further includes a transmission member. The power member is a linear motion motor. The plunger member includes a plunger rod and a plunger sleeve. The plunger rod is matched with the plunger sleeve. The connection port is opened at the bottom of the plunger sleeve. One end of the transmission member is connected to the output end of the linear motion motor, and the other end is connected to the plunger rod; The transmission member includes a guiding block and a transmission block. The guiding block has a first end and a second end. The first end and the second end are at a right angle. The first end is slidably matched with the outer shell of the linear motion motor, and the second end is connected to the output end of the linear motion motor. The transmission block is fixedly connected to the second end of the guiding block, and the transmission block is simultaneously connected to one end of the plunger rod. A position detector is arranged inside the main body chamber, and a baffle is arranged on the guiding block. The position detector detects the position where the baffle is located; The driving mechanism, the connecting pipe, and the handheld part are filled with liquid, and the plunger sleeve and the connection channel of the handheld part are filled with liquid; The liquid used is liquid paraffin oil; An oviduct stripping tube is connected to the other end of the connection channel of the gripping portion.

2. The sample precision controller according to claim 1, wherein The main body chamber is further equipped with an adapter. The adapter is connected to the lower end of the plunger sleeve, and the connection port is located inside the adapter. The connecting pipe is communicated with the adapter.

3. The sample precision controller according to claim 1 or 2, characterized in that, A placing member is arranged on the outside of the main body chamber. The two side portions of the placing member protrude, and the handheld part can be placed between the protrusions of the placing member.

4. The sample precision controller according to claim 1 or 2, wherein The main body chamber is further provided with a foot pedal interface.

5. Method for using a sample precision controller, using the sample precision controller according to any one of claims 1 to 4, characterized in that, It includes the following steps: An oviduct stripping tube is installed on the gripping portion; The power supply of the main body chamber is turned on, and the display shows and starts the program; Grip the handheld part, move it to the pre-use position, start the driving mechanism, generate a pressure difference, the connecting pipe is communicated with the connection port, and under the conduction of the connecting pipe, the pressure difference is conducted to the connection channel of the gripping portion and then continues to be conducted to the oviduct stripping tube; Due to the action of the pressure difference, the oviduct stripping tube can suck and spray liquid or liquid containing egg cells.

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