A microfluidic PCR chip and PCR detection method

Abstract

The present invention discloses a microfluidic PCR chip and a PCR detection method, which reacts in a closed state to avoid exchange with the outside air. The chip includes: a body with multiple functional cavities; a rotating chamber, which is rotatably arranged in the body, a transfer chamber is formed therein, and a first connecting port connected to the transfer chamber is provided on the rotating chamber; and a piston, a closed piston chamber is provided on the body, the piston is movably arranged in the piston chamber, and the piston chamber is divided into a first chamber and a second chamber isolated from each other by the piston; the body is also provided with multiple liquid flow channels respectively connected to the multiple functional chambers, a first air flow channel connecting the first chamber and the transfer chamber, and a second air flow channel connecting the second chamber and the internal space of the body except the piston chamber; one end of each liquid flow channel extends to the rotating chamber respectively, and when the rotating chamber is in any working position, one of the liquid flow channels is aligned with the first connecting port so that the corresponding functional chamber is connected to the transfer chamber.

Description

Technical Field

[0001] The invention belongs to the technical field of biological detection, and relates to a microfluidic PCR chip and a PCR detection method. Background Art

[0002] Microfluidic chips transplant the basic operation units such as sample preparation, mixing, reaction, separation, detection, cell culture, sorting, lysis, etc. involved in the fields of chemistry or biology onto a very small chip, and construct a microchannel network throughout the chip. Microfluidic chips can be used as biological or chemical microreactors or microsystems. Nucleic acid detection has the characteristics of high sensitivity and good specificity, and occupies an extremely important position in life sciences and medical testing. Combining microfluidic chip technology with nucleic acid detection technology, a microfluidic PCR chip that can be used for high-throughput and fully automatic nucleic acid analysis and detection of biological samples has been developed, which has the advantages of low reagent consumption, fast analysis speed, high detection throughput, high degree of automation, and use by non-professionals. It is particularly suitable for multi-index rapid quantitative detection of nucleic acid molecules in biological samples. However, the functional cavity of the existing PCR chip needs to be exchanged with the external gas, and the exhaust gas is discharged directly or after filtration from the chip body, which poses a risk of polluting the environment. Summary of the invention

[0003] The purpose of the present invention is to provide a microfluidic PCR chip, which can integrate nucleic acid extraction, amplification and detection, and react in a closed state to avoid exchange with the outside air, and is safe to use.

[0004] In order to achieve the above object, the present invention adopts the following technical scheme:

[0005] A microfluidic PCR chip, comprising a body with a plurality of functional cavities, the microfluidic chip further comprising:

[0006] A rotating chamber, which is rotatably disposed in the body, a transfer chamber is formed in the rotating chamber, and a first communication port communicating with the transfer chamber is opened on the rotating chamber; and

[0007] A piston, which is used to drive the liquid to flow between the functional chamber and the transfer chamber. The body is provided with a closed piston chamber, the piston is movably arranged in the piston chamber, and the piston chamber is divided into a first chamber and a second chamber isolated from each other by the piston;

[0008] The main body is provided with a plurality of liquid flow channels respectively connected to the plurality of functional chambers, a first air flow channel connecting the first chamber and the transfer chamber, and a second air flow channel connecting the second chamber and the internal space of the main body except the piston chamber;

[0009] One end of each of the liquid flow channels extends to the rotating chamber, and the rotating chamber has a plurality of working positions. In any of the working positions, the liquid flow channel of one of the functional chambers is aligned with the first connecting port so that the corresponding functional chamber is connected to the transfer chamber.

[0010] The internal space refers to the space in the body that is not connected to the outside. More preferably, the internal space includes the functional cavity, the microchannel connected to the functional cavity and the gap between the functional cavities. The internal space does not include the piston cavity and the cavity connected to the external space.

[0011] In some preferred embodiments, an annular connecting groove is further provided on the main body, and a second connecting port connected to the connecting groove is provided on the rotating bin. The shape of the connecting groove is the same as the rotation path of the second connecting port so that the connecting groove is connected to the transfer chamber in any of the working positions, and the first air flow channel is connected to the connecting groove.

[0012] In some preferred embodiments, the first communication port is located outside the communication groove.

[0013] In some preferred embodiments, the functional chamber includes a reaction chamber, and the second airflow channel is connected to the reaction chamber.

[0014] In some preferred embodiments, there are multiple reaction chambers, and the microfluidic chip includes a control valve capable of connecting or blocking the reaction chamber and the transfer chamber.

[0015] In some preferred embodiments, a plurality of third air flow channels are further provided on the main body, each of the reaction chambers is respectively connected to one of the liquid flow channels and one of the third air flow channels, a plurality of first microchannels and a plurality of second microchannels corresponding to the plurality of reaction chambers are respectively provided on the control valve, the transfer chamber and the piston chamber are located on one side of the control valve, and the reaction chamber is located on the other side of the control valve, the control valve is movably inserted in the main body and has a connecting position and a blocking position, in the connecting position, the liquid flow channel of the reaction chamber and the corresponding first microchannel are connected and connected, and its third air flow channel is connected to the second air flow channel by the corresponding second microchannel; in the blocking position, the liquid flow channel of the reaction chamber and the first microchannel deviate from each other, and the third air flow channel and the second microchannel deviate from each other.

[0016] In some preferred embodiments, a plurality of the first microchannels and a plurality of the second microchannels are arranged in parallel.

[0017] In some preferred embodiments, the first communication port has a circular rotating path, one end of each of the liquid flow channels extends to the rotating path, and the liquid flow channels are spaced around the rotating path.

[0018] In some preferred embodiments, the functional chamber includes a purification chamber, a magnetic rotor is disposed in the purification chamber, and the microfluidic chip further includes a magnet device for driving the magnetic rotor to move.

[0019] In some preferred embodiments, the body includes a base layer and a cover layer, the functional cavity is opened on the base layer, the functional cavity includes a sample chamber, the cover layer is covered on the base layer and the two are sealed, and the cover layer is provided with an injection hole connected to the sample chamber.

[0020] The present invention also adopts the following technical solution:

[0021] A PCR detection method, using the microfluidic PCR chip as described above, the PCR detection method comprises the following steps:

[0022] A. Rotate the rotating chamber to connect the first connecting port with the first functional chamber. At this time, the second air flow channel is connected with the second functional chamber. Move the piston to allow the liquid to flow from the first functional chamber into the transfer chamber of the rotating chamber or from the transfer chamber into the first functional chamber.

[0023] In some preferred embodiments, the PCR detection method further comprises the following steps:

[0024] B. Move the control valve to connect the first communication port with the reaction chamber in the functional chamber through the corresponding liquid flow channel. At this time, the second gas flow channel is connected with the reaction chamber. Move the piston to allow the liquid to flow from the transfer chamber into the reaction chamber.

[0025] C. Move the control valve to disconnect the liquid flow channel of the reaction chamber, and disconnect the gas flow channel from the reaction chamber.

[0026] The present invention adopts the above solution, which has the following advantages compared with the prior art:

[0027] The microfluidic PCR chip and PCR detection method of the present invention selectively connect the functional cavity and the transfer cavity by rotating the rotating chamber, and provide positive pressure or negative pressure to the transfer cavity by moving the piston, thereby realizing pipetting; and the two cavities on both sides of the piston are connected with the internal cavity of the body, gas circulation is realized in the chip body, nucleic acid extraction and nucleic acid amplification are performed in a fully sealed state, and environmental pollution and toxic and harmful substances entering the air through exhaust gas are avoided, and a series of functions such as nucleic acid extraction, purification, amplification and detection are integrated, and the operation is simple and safe to use. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] In order to more clearly illustrate the technical solution of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0029] Figure 1 It is a three-dimensional schematic diagram of a microfluidic PCR chip according to an embodiment of the present invention at one viewing angle.

[0030] Figure 2 yes Figure 1 The shown three-dimensional schematic diagram of the microfluidic PCR chip from another perspective.

[0031] Figure 3 yes Figure 1 Top view of the microfluidic PCR chip shown.

[0032] Figure 4 yes Figure 1 Front view of the microfluidic PCR chip shown.

[0033] Figure 5 yes Figure 1 A top view of the base layer and the rotation chamber of the microfluidic PCR chip.

[0034] Figure 6 yes Figure 1 Bottom view of the microfluidic PCR chip.

[0035] Figure 7 yes Figure 1 Schematic diagram of the microchannels within the microfluidic PCR chip shown.

[0036] Figure 8 yes Figure 1 Schematic diagram of the substrate layer of the microfluidic PCR chip.

[0037] Fig. 9 yes Figure 1 A perspective view of the control valves of the microfluidic PCR chip is shown.

[0038] in,

[0039] 1. body; 11. base layer; 110. step hole; 111. step surface; 112. transfer hole; 113. connecting groove; 12. cover layer; 121. injection hole; 13. piston;

[0040] 20, sample chamber; 21, reagent chamber; 22, purification chamber; 23, waste liquid chamber; 24, reaction chamber; 251, first chamber; 252, second chamber; 26, liquid flow channel; 27, first air flow channel; 28, second air flow channel; 29, third air flow channel;

[0041] 3. Rotating chamber; 30. Transfer chamber; 31. First communication port; 32. Second communication port; 33. Engagement groove;

[0042] 4. control valve; 41. first microchannel; 42. second microchannel;

[0043] 5. Magnet device. DETAILED DESCRIPTION

[0044] The preferred embodiments of the present invention are described in detail below in conjunction with the accompanying drawings so that the advantages and features of the present invention can be more easily understood by those skilled in the art. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0045] As shown in this specification and claims, the terms "comprises" and "include" only indicate the inclusion of the steps and elements that have been clearly identified, and these steps and elements do not constitute an exclusive list, and the method or device may also include other steps or elements. The term "and / or" as used herein includes any combination of one or more of the relevant listed items.

[0046] It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature, or it may be indirectly fixed or connected to the other feature. In addition, the descriptions of up, down, left, right, etc. used in the present invention are only relative to the relative positional relationship of the components of the present invention in the drawings.

[0047] It is further understood that in the present disclosure, "plurality" refers to two or more than two, and other quantifiers are similar thereto.

[0048] This embodiment provides a microfluidic PCR chip that integrates nucleic acid extraction, purification, amplification, and detection. Figures 1 to 9 As shown, the microfluidic PCR chip includes a body 1 with multiple functional cavities, a rotating chamber 3 rotatably arranged in the body 1, and a piston 13 for driving the flow of liquid. The functional cavity refers to a chamber in the body 1 for placing reagents and materials required for the PCR reaction, providing a reaction area required for the reaction, etc. A transfer cavity 30 is formed in the rotating chamber 3, and a first connecting port 31 connected to the transfer cavity 30 is formed on the rotating chamber 3. By rotating the rotating chamber 3, the transfer cavity 30 can be connected to any functional cavity through a liquid flow channel, thereby realizing liquid transfer.

[0049] The body 1 is provided with a closed piston chamber, in which the piston 13 is movably disposed, and can perform reciprocating linear motion in the piston chamber. The piston chamber is divided by the piston 13 into a first chamber 251 and a second chamber 252 that are isolated from each other. As the piston 13 moves, the first chamber 251 decreases and the second chamber 252 increases, or the first chamber 251 increases and the second chamber 252 decreases. By moving the piston 13, positive pressure or negative pressure can be provided to the transfer chamber 30, that is, power for liquid circulation can be provided.

[0050] The body 1 is also provided with a plurality of liquid flow channels 26 respectively connected to the plurality of functional chambers, a first air flow channel 27 connecting the first chamber 251 and the transfer chamber 30, and a second air flow channel 28 connecting the second chamber 252 and the internal space of the body 1 except the piston chamber. One end of each of the liquid flow channels 26 extends to the rotating chamber 3, and the rotating chamber 3 has a plurality of working positions. In any of the working positions, one of the liquid flow channels 26 is aligned with the first communication port 31 so that the corresponding functional chamber is connected to the transfer chamber 30, and the second air flow channel 28 is connected to the internal space of the body 1 that is not connected to the outside. The body 1 is also provided with an annular communication groove 113, and the rotating chamber 3 is provided with a second communication port 32 connected to the communication groove 113. The shape of the communication groove 113 is the same as the rotation path of the second communication port 32 so that the communication groove 113 is connected to the transfer chamber 30 in any of the working positions, and the first air flow channel 27 is connected to the communication groove 113. That is to say, no matter where the rotating chamber 3 rotates to, the first airflow channel 27 always connects the first chamber 251 of the piston chamber with the transfer chamber 30 through the connecting groove 113 and the second connecting port 32. When the piston 13 moves, the gas pressures of the above-mentioned multiple functional chambers on the body 1 can be balanced through the gaps in the body 1. The above-mentioned internal space includes the above-mentioned functional chambers, the microchannels connected to the functional chambers, and the gaps between the functional chambers, but does not include the piston chamber and the cavity connected to the external space.

[0051] The chip body 1 is in the shape of a flat plate placed horizontally; the rotating chamber 3 is rotatably embedded in the body 1, and its rotation axis extends vertically; the piston 13 is inserted in the body 1 so as to be movable in the horizontal direction, and one end of the piston 13 preferably extends outside the body 1, so as to facilitate the external drive device for driving the piston 13 to move. The number of the first communication port 31 is one, and it rotates along a circular rotation path; the number of the second communication ports 32 can be one, two or more, and the distance from the rotation axis is equal. The first communication port 31 and the second communication port 32 are both opened on the bottom wall of the rotating chamber 3, and the second communication port 32 is located on the inner side of the first communication port 31; the rotation path of the second communication port 32 is also circular and forms concentric rings of different radii with the rotation path of the first communication port 31. The communication groove 113 is located below the second communication port 32. The first airflow channel 27 is opened in the body 1, one end of which is connected to the communication groove 113, and the other end is connected to the first cavity 251 of the piston 13. One end of each of the liquid flow channels 26 extends to the rotation path of the first communication port 31 and is arranged at intervals around the cover rotation path.

[0052] The functional chamber includes a sample chamber 20, a plurality of reagent chambers 21, a purification chamber 22, a waste liquid chamber 23 and a plurality of reaction chambers 24; the plurality of reaction chambers 24 are arranged side by side on one side of the rotating chamber 3, and the other functional chambers are evenly arranged around the other side of the rotating chamber 3. The microfluidic PCR chip includes a control valve 4 that can connect or block the reaction chamber 24 and the transfer chamber 30. Specifically in this embodiment, the body 1 is also provided with a plurality of third air flow channels 29, and each of the reaction chambers 24 is respectively connected with a liquid flow channel 26 and a third air flow channel 29. The control valve 4 is provided with a plurality of first microchannels 41 and a plurality of second microchannels 42 corresponding to the plurality of reaction chambers 24, respectively. The first microchannel 41 can block or connect the liquid flow channel 26 of the reaction chamber 24. When it is aligned with a certain liquid flow channel 26, the liquid flow channel 26 is connected, and when it is misaligned with the liquid flow channel 26, the liquid flow channel 26 is blocked by the control valve 4. The transfer chamber 30 and the piston chamber are located on one side of the control valve 4, and the reaction chamber 24 is located on the other side of the control valve 4. The control valve 4 is movably inserted in the body 1 and has a connecting position and a blocking position. In the connecting position, the liquid flow channel 26 of the reaction chamber is connected and connected with the corresponding first microchannel 41, and its third air flow channel 29 is connected to the second air flow channel 28 by the corresponding second microchannel 42, and the second air flow channel 28 is connected with each second microchannel 42; in the blocking position, the liquid flow channel 26 of each reaction chamber deviates from the first microchannel 41, and the third air flow channel 29 deviates from the second microchannel 42. The control valve 4 can be switched between the connecting position and the blocking position by moving one end distance.

[0053] The control valve 4 is movably disposed in the body 1 in the horizontal direction, and a plurality of reaction chambers 24 are sequentially arranged side by side on the same side of the control valve 4 along the moving direction of the control valve 4. A plurality of the first microchannels 41 and a plurality of the second microchannels 42 are arranged in parallel. Specifically, the first microchannels 41 and the second microchannels 42 are parallel to each other, such as extending in the up-down direction. A plurality of first holes are provided on the upper surface of the control valve 4, and a plurality of second holes are provided on the lower surface of the control valve 4. The positions and numbers of the first holes and the second holes correspond one to one, and a corresponding group of the first holes and the second holes serve as the inlet and outlet of a first microchannel 41 or a second microchannel 42, respectively.

[0054] The body 1 includes a base layer 11 and a cover layer 12. The materials of the cover layer 12 and the base layer 11 can be polymers such as PS, PMMA, PDMA, and PC. The processing methods of the cover layer 12 and the base layer 11 include but are not limited to photolithography, injection molding, machining, laser cutting, etc.; the connection methods of the two include but are not limited to ultrasonic bonding, thermal bonding, anodic bonding, low-temperature bonding, etc. The thickness of the cover layer 12 and the base layer 11 are 1-20 mm respectively. A step hole 110 is provided in the middle of the cover layer 12, and the aperture of the lower part is smaller than the aperture of the upper part. The step surface 111 of the step hole 110 is respectively provided with a pipetting hole 112 corresponding to each liquid flow channel 26 and the above-mentioned connecting groove 113. The rotating bin 3 is rotatably embedded in the step hole 110. The bottom surface of the rotating bin 3 is provided with a coupling groove 33 for an external drive device, and the external drive device can be a motor for driving the rotating bin 3 to rotate.

[0055] The functional cavity and the piston cavity are both provided on the base layer 11. The cover layer 12 is covered on the base layer 11 and the two are sealed. The cover layer 12 is provided with an injection hole 121 connected to the sample cavity 20; after injection, the injection hole 121 can be sealed by a cover or a film. In addition, there is a space between the cover layer 12 and the base layer 11 that enables the functional cavities to be connected to achieve air pressure balance between the functional cavities. The reagents required for the PCR reaction, such as lysate, purification agent, eluent, etc., are pre-placed in the reagent cavity 21. Preferably, in this embodiment, the purification agent is isopropanol. A magnetic rotor and magnetic beads are provided in the purification cavity 22, and the microfluidic PCR chip also includes a magnet device 5 for driving the magnetic rotor to move, and the magnet device 5 is close to the purification cavity 22. The waste liquid cavity 23 is used to recover waste liquid generated during nucleic acid extraction and the like.

[0056] This embodiment also provides a PCR detection method, using the microfluidic PCR chip as described above. The PCR detection method comprises the following steps:

[0057] A. Rotate the rotating chamber 3 to make the first connecting port 31 connected with the first functional chamber. At this time, the second air flow channel 28 is connected with the second functional chamber. Move the piston 13 to make the liquid flow from the first functional chamber into the transfer chamber 30 of the rotating chamber 3 or from the transfer chamber 30 into the first functional chamber.

[0058] The PCR detection method also includes the following steps:

[0059] B. Move the control valve 4 to connect the first communication port 31 with the reaction chamber 24 in the functional chamber through the corresponding liquid flow channel 26. At this time, the second gas flow channel 28 is connected with the reaction chamber 24. Move the piston 13 to allow the liquid to flow from the transfer chamber 30 into the reaction chamber 24.

[0060] C. Move the control valve 4 to disconnect the liquid flow channel 26 of the reaction chamber 24, and disconnect the gas flow channel and the reaction chamber 24.

[0061] The principles and implementation process of nucleic acid extraction, purification, amplification and detection of the above-mentioned microfluidic PCR chip are as follows:

[0062] 1. The rotating chamber 3 rotates on the main body 1 and rotates to different angles. The first communication port 31 of the rotating chamber 3 will be connected to the liquid flow channels 26 of different functional chambers on the main body 1.

[0063] 2. After the above-mentioned liquid flow channel 26 is connected, the piston 13 is moved to realize the transmission of the liquid; by accurately moving the piston 13, the precise quantitative function can be realized.

[0064] 3. After the reagent is added to the purification chamber 22, the magnet device 5 is rotated, and the magnetic rotor in the purification chamber 22 rotates accordingly, thereby achieving mixing of the magnetic beads.

[0065] 4. After the liquid is transferred to the 12 reaction chambers 24, the control valve 4 is moved to completely isolate the reagents in the reaction chambers 24 from the internal cavity.

[0066] 5. The chambers and microchannels on the base layer 11 are completely sealed by the cover layer 12, and all liquid transmission is achieved through internal circulation through the piston 13 to prevent contamination.

[0067] 6. The PCR reagent amplification reaction is achieved through temperature cycle heating, and then the optical module is used for detection to achieve the extraction, amplification and detection of nucleic acid.

[0068] The present embodiment is an integrated multi-channel microfluidic PCR chip, which not only integrates a series of functions such as nucleic acid extraction, purification, amplification and detection, but also performs nucleic acid extraction and nucleic acid amplification in a fully sealed state to avoid environmental pollution; it also realizes multi-channel detection requirements, and has the characteristics of simple operation process, low reagent consumption, fast analysis speed, etc., and is particularly suitable for high-throughput, fully automatic nucleic acid analysis and detection of biological samples.

[0069] The above embodiment is only for illustrating the technical concept and features of the present invention, and is a preferred embodiment. Its purpose is to enable people familiar with the technology to understand the content of the present invention and implement it accordingly, and it cannot be used to limit the protection scope of the present invention. Any equivalent transformation or modification made according to the principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A microfluidic PCR chip, comprising a body having a plurality of functional cavities, characterized in that: The microfluidic chip also includes: A rotating chamber, which is rotatably disposed in the body, a transfer chamber is formed in the rotating chamber, and a first communication port communicating with the transfer chamber is opened on the rotating chamber; and A piston, which is used to drive the liquid to flow between the functional chamber and the transfer chamber. The body is provided with a closed piston chamber, the piston is movably arranged in the piston chamber, and the piston chamber is divided into a first chamber and a second chamber isolated from each other by the piston; The main body is provided with a plurality of liquid flow channels respectively connected to the plurality of functional chambers, a first air flow channel connecting the first chamber and the transfer chamber, and a second air flow channel connecting the second chamber and the internal space of the main body except the piston chamber; One end of each of the liquid flow channels extends to the rotating chamber, and the rotating chamber has a plurality of working positions. In any of the working positions, the liquid flow channel of one of the functional chambers is aligned with the first connecting port so that the corresponding functional chamber is connected to the transfer chamber.

2. The microfluidic PCR chip according to claim 1, characterized in that: The main body is also provided with an annular connecting groove, and the rotating bin is provided with a second connecting port which is interconnected with the connecting groove. The shape of the connecting groove is the same as the rotation path of the second connecting port so that the connecting groove is connected with the transfer chamber in any of the working positions, and the first air flow channel is connected with the connecting groove.

3. The microfluidic PCR chip according to claim 2, characterized in that: The first communication port is located outside the communication groove.

4. The microfluidic PCR chip according to claim 1, characterized in that: The functional chamber includes a reaction chamber, and the second air flow channel is communicated with the reaction chamber.

5. The microfluidic PCR chip according to claim 4, characterized in that: There are multiple reaction chambers, and the microfluidic chip includes a control valve that can connect or block the reaction chamber and the transfer chamber.

6. The microfluidic PCR chip according to claim 5, characterized in that: The main body is also provided with a plurality of third air flow channels, each of the reaction chambers is respectively connected to one of the liquid flow channels and one of the third air flow channels, the control valve is provided with a plurality of first microchannels and a plurality of second microchannels corresponding to the plurality of reaction chambers, the transfer chamber and the piston chamber are located on one side of the control valve, and the reaction chamber is located on the other side of the control valve, the control valve is movably inserted in the main body and has a connecting position and a blocking position, in the connecting position, the liquid flow channel of the reaction chamber is connected and connected to the corresponding first microchannel, and its third air flow channel is connected to the second air flow channel by the corresponding second microchannel; in the blocking position, the liquid flow channel of the reaction chamber and the first microchannel deviate from each other, and the third air flow channel and the second microchannel deviate from each other.

7. The microfluidic PCR chip according to claim 6, characterized in that: A plurality of the first microchannels and a plurality of the second microchannels are arranged in parallel.

8. The microfluidic PCR chip according to claim 1, characterized in that: The first communication port has a circular rotating path, one end of each of the liquid flow channels extends to the rotating path, and the liquid flow channels are arranged at intervals around the rotating path.

9. The microfluidic PCR chip according to claim 1, characterized in that: The functional cavity comprises a purification cavity, a magnetic rotor is arranged in the purification cavity, and the microfluidic chip further comprises a magnet device for driving the magnetic rotor to move.

10. The microfluidic PCR chip according to claim 1, characterized in that: The body comprises a base layer and a cover layer, the functional cavity is opened on the base layer, the functional cavity comprises a sample chamber, the cover layer is covered on the base layer and the two are sealed, and the cover layer is provided with an injection hole connected with the sample chamber.

11. A PCR detection method, characterized in that: Using the microfluidic PCR chip according to any one of claims 1 to 10, the PCR detection method comprises the following steps: A. Rotate the rotating chamber to connect the first connecting port with the first functional chamber. At this time, the second air flow channel is connected with the second functional chamber. Move the piston to allow the liquid to flow from the first functional chamber into the transfer chamber of the rotating chamber or from the transfer chamber into the first functional chamber.

12. The PCR detection method according to claim 11, characterized in that: The PCR detection method also includes the following steps: B. Move the control valve to connect the first communication port with the reaction chamber in the functional chamber through the corresponding liquid flow channel. At this time, the second gas flow channel is connected with the reaction chamber. Move the piston to allow the liquid to flow from the transfer chamber into the reaction chamber. C. Move the control valve to disconnect the liquid flow channel of the reaction chamber, and disconnect the gas flow channel from the reaction chamber.

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