Preimplantation genetic diagnosis embryo biopsy device

Abstract

The present invention discloses a pre-implantation genetic diagnosis embryo biopsy device, comprising an incubation table, a transparent cover and a rotating seat, wherein a plurality of culture stations are arranged on the incubation table, each culture station having an incubation tank suitable for placing a culture dish and a sampling tank suitable for inserting a sampling tube; the transparent cover is located above the incubation table, and the transparent cover is provided with an opening suitable for revealing a culture station; the rotating seat is arranged at the bottom of the incubation table, and is used to drive the incubation table to rotate relative to the transparent cover, so as to selectively switch one of the culture stations to the opening. The pre-implantation genetic diagnosis embryo biopsy device provided according to an embodiment of the present invention can be used for biopsy sampling, ensure the correlation between the sample and the embryo in the culture dish, and reduce the possible risks caused by human operation errors.

Description

Technical Field

[0001] The present invention relates to the technical field of medical equipment, and in particular to a pre-implantation genetic diagnosis embryo biopsy device. Background Art

[0002] Preimplantation Genetic Diagnosis (PGD) is based on in vitro fertilization and embryo transfer technology. It combines micromanipulation technology, embryology, genetics and molecular biology research. Before the embryo is implanted into the uterine cavity, it uses DNA analysis technology to diagnose chromosomal abnormalities or genetic diseases in the microbiopsied embryo cells, selects normal embryos for implantation, and thus prevents the birth of children with genetic diseases.

[0003] During the preimplantation genetic diagnosis process, the biopsy sample and the cultured embryo need to be associated and marked to prevent the biopsy result from not matching the embryo, leading to errors in embryo transplantation and ultimately irreparable consequences. Currently, the sample and embryo are usually coded and associated, that is, the culture dish and the sampling tube are associated and marked. However, in some cases, during the biopsy process, the doctor may inject the sample obtained from the culture dish into an unrelated sampling tube with a certain probability, which poses a certain risk. Summary of the invention

[0004] The present invention aims to solve one of the technical problems in the related art at least to a certain extent. To this end, the purpose of the present invention is to provide a preimplantation genetic diagnosis embryo biopsy device.

[0005] To achieve the above-mentioned purpose, a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention comprises:

[0006] An incubation platform, wherein a plurality of incubation stations are provided on the incubation platform, each of which has an incubation tank suitable for placing a culture dish and a sampling tank suitable for inserting a sampling tube;

[0007] A transparent cover, the transparent cover is located above the incubation platform, and the transparent cover is provided with an opening suitable for revealing a culture station;

[0008] A rotating seat is arranged at the bottom of the incubation table and is used to drive the incubation table to rotate relative to the transparent cover so as to selectively switch one of the culture stations to the opening.

[0009] According to the embryo biopsy device for preimplantation genetic diagnosis provided by the embodiment of the present invention, the transparent cover is arranged above the incubation platform, the rotating seat can drive the incubation platform to rotate relative to the transparent cover, and then selectively switch the last culture station of the incubation platform to rotate to the opening on the transparent cover, and each culture station has an incubation tank and a sampling tank, a culture dish can be placed in the incubation tank, and a sampling tube can be placed in the sampling tank, so, in the biopsy process, after placing the culture dish and the sampling tube associated with each other on each culture station, and because the opening can only reveal one culture station, therefore, the sample taken out from the culture dish can only be injected into the associated sampling tube, ensuring the correlation between the sample and the embryo in the culture dish, reducing the possible risk caused by human error. Further, by rotating each culture station for biopsy, the biopsy efficiency is improved. In addition, the incubation platform can provide a suitable culture temperature environment, which is conducive to embryo culture.

[0010] In addition, the preimplantation genetic diagnosis embryo biopsy device according to the above embodiment of the present invention may also have the following additional technical features:

[0011] According to one embodiment of the present invention, the rotating seat comprises:

[0012] Base;

[0013] A rotating disk, the rotating disk is pivotally arranged on the base around its own axis, and the incubation table is fixed on the rotating disk and can rotate with the rotating disk;

[0014] A turbine and a worm, wherein the turbine is arranged at the bottom of the rotating disk and is coaxial with the rotating disk, the worm is arranged in the base and can pivot around its own axis, and the worm is meshed with the turbine;

[0015] A driving motor is connected to the worm to drive the worm to rotate.

[0016] According to one embodiment of the present invention, a accommodating cavity is provided on the top surface of the base, a cylindrical boss is provided on the bottom of the accommodating cavity, a sleeve portion is provided on the bottom of the rotating disk, and the sleeve portion is sleeved on the cylindrical boss so that the rotating disk can pivot around its own axis relative to the base.

[0017] According to one embodiment of the present invention, a through hole is provided at the center of the incubation table, and a positioning piece is provided on the cylindrical boss;

[0018] A downwardly protruding connecting column is provided at the bottom of the transparent cover, a positioning hole is provided at the bottom of the connecting column, the connecting column passes through the through hole, and the positioning hole is sleeved with the positioning piece to fix the transparent cover and the base relatively in the circumferential direction.

[0019] According to one embodiment of the present invention, the incubation station comprises:

[0020] A base, the base is fixed on the rotating base, the base is provided with the through hole, a plurality of the sampling grooves surrounding the outside of the through hole, and an annular groove surrounding the outside of the plurality of the sampling grooves, and the plurality of the sampling grooves are arranged at equal intervals;

[0021] A heating film, the heating film is formed in an annular shape and is arranged in the annular groove;

[0022] A heat conducting plate is formed in a ring shape and is attached above the heating film. A plurality of the incubation grooves are arranged on the heat conducting plate. The plurality of the incubation grooves are arranged at equal intervals and correspond one-to-one to the plurality of the sampling grooves.

[0023] According to one embodiment of the present invention, the base comprises:

[0024] A cylindrical portion, wherein the through hole is arranged at the center of the cylindrical portion, and a plurality of sampling slots are arranged on the top surface of the cylindrical portion and surround the through hole;

[0025] The annular portion is formed by protruding radially outward from the upper end of the cylindrical portion, and the annular groove is arranged on the top surface of the annular portion.

[0026] According to an embodiment of the present invention, the upper end of the cylindrical portion protrudes radially toward the annular groove to form a positioning portion, and the inner edge of the heat conducting plate is provided with a positioning opening that cooperates with the positioning portion.

[0027] According to one embodiment of the present invention, a plurality of optical coupling baffles are arranged at intervals on the outer circumference of the rotating disk, and the plurality of optical coupling baffles correspond one-to-one to the plurality of the culture stations;

[0028] The base is provided with an upwardly extending bracket, and the bracket is provided with a photoelectric switch. Each time the rotating disk rotates a predetermined angle, one of the plurality of optical coupling baffles rotates to the photoelectric switch in rotation, so that the photoelectric switch generates a trigger signal, and the trigger signal is used to control the drive motor to stop, so that one of the plurality of culture stations switches to the opening.

[0029] According to one embodiment of the present invention, the upper end of the accommodating cavity is open to form an opening, the opening is provided with a lip edge protruding radially inward, and the lip edge is provided with an elastic power supply contact connected to the power source;

[0030] A conductive ring is provided on the bottom surface of the rotating disk, and the conductive ring is in electrical contact with the elastic power supply contact. The electrode of the heating film is provided at the bottom of the base and is electrically connected to the conductive ring.

[0031] According to an embodiment of the present invention, the cross-section of the positioning hole is T-shaped, and the cross-section shape of the positioning member is matched with the positioning hole.

[0032] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0034] Figure 1 1 is a schematic diagram of the structure of a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0035] Figure 2 is an exploded view of a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0036] Figure 3 is a partial exploded view of a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0037] Figure 4 is an exploded view of an incubation platform in a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0038] Figure 5 is an exploded view of a rotating seat in a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0039] Figure 6 It is a schematic structural diagram of a rotating disk in a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0040] Figure 7 It is a schematic structural diagram of a transparent cover in a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention;

[0041] Figure 8 It is a cross-sectional view of a base in a preimplantation genetic diagnosis embryo biopsy device according to an embodiment of the present invention.

[0042] Reference numerals:

[0043] 10. Incubation platform;

[0044] 101. Base;

[0045] 101a, cylindrical portion;

[0046] 1011a, positioning unit;

[0047] 101b, annular portion;

[0048] H11, sampling trough;

[0049] H12, through hole;

[0050] H13, annular groove;

[0051] 102. Heating film;

[0052] 103. Heat conducting plate;

[0053] 1031, positioning port;

[0054] H10, incubation tank;

[0055] 104. Optocoupler baffle;

[0056] 20. Transparent cover;

[0057] 201, connecting column;

[0058] 2011, positioning hole;

[0059] H20, opening;

[0060] 30. Rotating seat;

[0061] 301, base;

[0062] 3011, lip edge;

[0063] 3012, elastic power supply contact;

[0064] 302, rotating disk;

[0065] 3021, sleeve part;

[0066] 3022, conductive ring;

[0067] 303, turbine;

[0068] 304, worm;

[0069] 305, driving motor;

[0070] 306, cylindrical boss;

[0071] 3061, positioning piece;

[0072] 307, bracket;

[0073] 308. Photoelectric switch;

[0074] 40. Petri dish;

[0075] 50. Sampling tube.

[0076] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0077] The embodiments of the present invention are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but are not to be construed as limiting the present invention. All other embodiments obtained by ordinary technicians in the field without creative work based on the embodiments of the present invention are within the scope of protection of the present invention.

[0078] In the description of the present invention, it is to be understood that the terms “center”, “longitudinal”, “lateral”, “length”, “width”, “thickness”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside”, “outside”, “clockwise”, “counterclockwise”, “axial”, “circumferential”, “radial”, etc., indicating orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0079] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features. In the description of the present invention, the meaning of "plurality" is two or more, unless otherwise clearly and specifically defined.

[0080] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0081] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0082] The following describes in detail the embryo biopsy device for preimplantation genetic diagnosis according to an embodiment of the present invention with reference to the accompanying drawings.

[0083] Reference Figures 1 to 8 As shown, the preimplantation genetic diagnosis embryo biopsy device provided according to an embodiment of the present invention includes an incubation table 10, a transparent cover 20 and a rotating seat 30.

[0084] Specifically, a plurality of culture stations are arranged on the incubation platform 10, each of which has an incubation tank H10 suitable for placing a culture dish 40 and a sampling tank H11 suitable for inserting a sampling tube 50. Preferably, the plurality of culture stations are arranged circumferentially and spaced apart around an axis.

[0085] That is to say, each culture station can be placed with a culture dish 40 and a sampling tube 50, and the culture dish 40 and the sampling tube 50 can be associated by coding or other associated identification methods, that is, the culture dish 40 and the sampling tube 50 placed on each culture station are associated. In addition, the incubation tank H10 can heat the culture dish 40 placed therein and maintain a predetermined temperature, providing a suitable temperature environment for the embryo in the culture dish 40, which is conducive to the cultivation of the embryo and reduces the impact on the embryo during the biopsy process. The sampling tank H11 has a certain depth, which is convenient for the sampling tube 50 to be inserted into the sampling tank H11, and the sampling tube 50 is kept vertically fixed on the incubation table 10.

[0086] The transparent cover 20 is located above the incubation platform 10 , and an opening H20 suitable for revealing one culture station is provided on the transparent cover 20 , that is, the opening H20 on the transparent cover 20 can only reveal one culture station.

[0087] The rotating seat 30 is disposed at the bottom of the incubation table 10 to drive the incubation table 10 to rotate relative to the transparent cover 20 so as to selectively switch one of the culture stations to the opening H20 .

[0088] That is to say, the rotating seat 30 can drive the incubation table 10 to rotate relative to the transparent cover 20. Since the opening H20 on the transparent cover 20 can only reveal one culture station, by rotating the incubation table 10, the culture station on the incubation table 10 can be switched to be opposite to the opening H20, and then one culture station can be selectively rotated to a position opposite to the opening H20, thereby facilitating biopsy sampling of the embryo on the culture station.

[0089] During the biopsy sampling process, the doctor can perform blastomere biopsy or blastocyst trophoblast cell biopsy under the micromanipulation system, take samples from the embryo in the culture dish 40 through the biopsy needle, and after successful sampling, inject the sample into the sampling tube 50.

[0090] According to the embryo biopsy device for preimplantation genetic diagnosis provided by the embodiment of the present invention, the transparent cover 20 is arranged above the incubation table 10, and the rotating seat 30 can drive the incubation table 10 to rotate relative to the transparent cover 20, thereby selectively switching the previous culture station of the incubation table 10 to rotate to the opening H20 on the transparent cover 20, and each culture station has an incubation tank H10 and a sampling tank H11, and the incubation tank H10 can be placed in the culture dish 40, and the sampling tank H11 can be placed in the sampling tube 50, so, in the biopsy process, after placing the culture dishes 40 and the sampling tube 50 with associated marks on each culture station, and since the opening H20 can only reveal one culture station, the sample taken from the culture dish 40 can only be injected into the associated sampling tube 50, ensuring the correlation between the sample and the embryo in the culture dish 40, and reducing the possible risks caused by human operation errors. Further, by rotating each culture station for biopsy, the biopsy efficiency is improved. In addition, the incubation platform 10 can provide a suitable culture temperature environment, which is conducive to embryo culture.

[0091] Reference Figure 5 As shown, in one embodiment of the present invention, the rotating seat 30 includes a base 301, a rotating disk 302, a turbine 303, a worm 304 and a driving motor 305, wherein the base 301 can be placed on a flat object such as a work surface, or the base 301 has a certain height and can be directly placed on the ground. The rotating disk 302 is pivotally arranged on the base 301 around its own axis, and the incubation table 10 is fixed on the rotating disk 302 and can rotate with the rotating disk 302.

[0092] The turbine 303 is arranged at the bottom of the rotating disk 302 and is coaxial with the rotating disk 302. The worm 304 is arranged in the base 301 and can pivot around its own axis. The worm 304 is meshed with the turbine 303. The driving motor 305 is connected to the worm 304 to drive the worm 304 to rotate.

[0093] That is, the output shaft of the driving motor 305 is connected to the worm 304, the worm 304 is meshed with the turbine 303, and the turbine 303 is coaxially fixed on the rotating disk 302, and the incubation table 10 is fixed on the rotating disk 302. When the driving motor 305 is working, the driving motor 305 can drive the worm 304 to rotate, and the worm 304 further drives the rotating disk 302 to rotate through the turbine 303, so that the incubation table 10 can rotate synchronously with the rotating disk 302, realizing the rotation drive of the incubation table 10, and its structure is simple, and the cooperation of the turbine 303 and the worm 304 can make the rotation drive reliable and stable.

[0094] Reference Figure 2 and Figure 5 As shown, in one example of the present invention, a accommodating cavity is provided on the top surface of the base 301, a cylindrical boss 306 is provided on the bottom of the accommodating cavity, a sleeve portion 3021 is provided on the bottom of the rotating disk 302, and the sleeve portion 3021 is sleeved on the cylindrical boss 306 so that the rotating disk 302 can pivot around its own axis relative to the base 301.

[0095] In this embodiment, the sleeve portion 3021 at the bottom of the rotating disk 302 is extended into the accommodating cavity and is socketed with the cylindrical boss 306 at the bottom of the accommodating cavity, thereby realizing the pivot connection between the rotating disk 302 and the base 301. In addition, the above-mentioned connection method is convenient for disassembly and assembly, and the rotation is stable and reliable.

[0096] In one embodiment of the present invention, a through hole H12 is provided at the center of the incubation table 10, and a positioning piece 3061 is provided on the cylindrical boss 306. A downwardly protruding connecting column 201 is provided at the bottom of the transparent cover 20, and a positioning hole 2011 is provided at the bottom of the connecting column 201. The connecting column 201 passes through the through hole H12, and the positioning hole 2011 is sleeved with the positioning piece 3061, so that the transparent cover 20 and the base 301 are relatively fixed in the circumferential direction.

[0097] During the assembly of the biopsy device, the rotating disk 302 can be rotated and matched with the cylindrical boss 306 at the bottom of the accommodating chamber through the sleeve portion 3021, and then the incubation table 10 can be fixed on the rotating disk 302 through the screw fasteners. Finally, the connecting column 201 at the bottom of the transparent cover 20 is passed through the through hole H12 on the incubation table 10 and then extended into the accommodating chamber. The positioning hole 2011 on the connecting column 201 is plugged into the positioning piece 3061 on the cylindrical boss 306 to achieve the installation and assembly of the transparent cover 20. In addition, the transparent cover 20 can be relatively fixed to the base 301. The structure is simple, the assembly is very convenient, and it is easy to disassemble and clean.

[0098] Preferably, the cross-section of the positioning hole 2011 is T-shaped, and the cross-sectional shape of the positioning member 3061 is adapted to the positioning hole 2011. In this way, the positioning hole 2011 with the T-shaped interface cooperates with the positioning member 3061, so that the transparent cover 20 and the base 301 can be relatively fixed in the circumferential direction, and need to be plugged and assembled in a certain direction to ensure that the opening H20 is always located in a fixed position.

[0099] Reference Figure 4 As shown, in some embodiments of the present invention, the incubation table 10 includes a base 101, a heating film 102 and a heat conducting plate 103. The base 101 is fixed on the rotating base 30. The base 101 is provided with the through hole H12, the plurality of sampling grooves H11 surrounding the outside of the through hole H12, and the annular grooves H13 surrounding the outside of the plurality of sampling grooves H11. The plurality of sampling grooves H11 are arranged at equal intervals.

[0100] The heating film 102 is formed in an annular shape and is disposed in the annular groove H13. The heat conducting plate 103 is formed in an annular shape and is attached above the heating film 102. The heat conducting plate 103 is provided with a plurality of the incubation grooves H10, which are arranged at equal intervals and correspond one to one with the plurality of the sampling grooves H11.

[0101] That is, a plurality of sampling grooves H11 are provided on the base 101, and are arranged at intervals between the through hole H12 and the annular groove H13, and the heating film 102 and the heat conducting plate 103 are stacked in the annular groove H13, and the heat conducting plate 103 is located above the heating film 102. Correspondingly, a plurality of incubation grooves H10 are arranged at intervals on the heat conducting plate 103. In use, the culture dish 40 can be placed in the incubation groove H10, and heated by the heating film 102. The heat of the heating film 102 is transferred to the heat conducting plate 103, and then transferred to the culture dish 40 through the heat conducting plate 103, so that the culture dish 40 can be incubated. In addition, the sampling tube 50 is inserted into the sampling hole of the base 101. Since the sampling hole is provided on the base 101, rather than directly on the heat conducting plate 103 or the heating film 102, the heating of the sampling tube 50 is reduced, and the sample is prevented from being improperly heated.

[0102] Reference Figure 4 and Figure 8 As shown, in one embodiment of the present invention, the base 101 includes a cylindrical portion 101a and an annular portion 101b, wherein a through hole H12 is provided at the center of the cylindrical portion 101a, and a plurality of sampling grooves H11 are provided on the top surface of the cylindrical portion 101a and surround the through hole H12. The annular portion 101b is formed by radially protruding outward from the upper end of the cylindrical portion 101a, and the annular groove H13 is provided on the top surface of the annular portion 101b.

[0103] That is to say, the base 101 is mainly composed of a cylindrical portion 101a and an annular portion 101b, and the height of the cylindrical portion 101a is greater than the height of the annular portion 101b. Since the sampling tube 50 is relatively long, the corresponding sampling groove H11 needs to have a greater depth, while the height dimension of the culture dish 40 is relatively small, and the depth of the corresponding incubation groove H10 is relatively shallow. Therefore, the sampling groove H11 is arranged on the cylindrical portion 101a, and the annular groove H13 is arranged on the annular portion 101b, which can easily meet the depth requirements of the incubation groove H10 and the sampling groove H11. In addition, the temperature transfer between the annular portion 101b and the cylindrical portion 101a is also slower.

[0104] Reference Figure 3 and Figure 4 As shown, in an example of the present invention, the upper end of the cylindrical portion 101a protrudes radially toward the annular groove H13 to form a positioning portion 1011a, and the inner edge of the heat conducting plate 103 is provided with a positioning opening 1031 that cooperates with the positioning portion 1011a.

[0105] That is to say, the positioning hole 1031 on the heat conducting plate 103 can cooperate with the positioning portion 1011a on the base 101, so that the heat conducting plate 103 is relatively fixed to the base 101 when installed in the annular groove H13, ensuring that the heat conducting plate 103 is reliably installed and easy to assemble. At the same time, it ensures that the incubation groove H10 on the heat conducting plate 103 will not shift, ensuring that the culture dish 40 and the sampling tube 50 always remain associated.

[0106] Reference Figures 1 to 4 As shown, in one embodiment of the present invention, a plurality of optical coupling baffles 104 are provided at intervals on the outer circumference of the rotating disk 302 , and the plurality of optical coupling baffles 104 correspond one-to-one to the plurality of the culture stations.

[0107] An upwardly extending bracket 307 is provided on the base 301, and a photoelectric switch 308 is provided on the bracket 307. Each time the rotating disk 302 rotates a predetermined angle, one of the multiple optical coupling baffles 104 rotates to the photoelectric switch 308 in rotation, so that the photoelectric switch 308 generates a trigger signal, and the trigger signal is used to control the drive motor 305 to stop, so that one of the multiple culture stations switches to the opening H20.

[0108] That is to say, when the driving motor 305 drives the rotating disk 302 to rotate, each time an optical coupling baffle 104 on the rotating disk 302 rotates to the photoelectric switch 308, the photoelectric switch 308 can generate a trigger signal, and the driving motor 305 stops working when receiving the trigger signal, so that the rotating disk 302 can stop rotating and remain at this position. Since the multiple optical coupling baffles 104 correspond to the multiple culture stations one by one, when the rotating disk 302 stops, a culture station on the incubation table 10 just rotates to the opening H20 of the transparent cover 20.

[0109] In other words, in this embodiment, the relative position of the plurality of optical coupling baffles 104 and the photoelectric switch 308 during rotation can realize automatic switching of the culture station to the opening H20, thereby ensuring reliable and stable switching of the culture station.

[0110] Reference Figure 5 and Figure 6 As shown, in one embodiment of the present invention, the upper end of the accommodating cavity is opened to form an opening, and the opening is provided with a lip edge 3011 protruding radially inward, and the lip edge 3011 is provided with an elastic power supply contact 3012 connected to the power source.

[0111] A conductive ring 3022 is provided on the bottom surface of the rotating disk 302 . The conductive ring 3022 is in electrical contact with the elastic power supply contact 3012 . The electrode of the heating film 102 is provided at the bottom of the base 101 and is electrically connected to the conductive ring 3022 .

[0112] In this embodiment, the heating film 102 can be stably and reliably powered by the conductive ring 3022 at the bottom of the rotating disk 302 and the elastic power supply contact 3012 on the base 301 .

[0113] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification and the features of the different embodiments or examples, without contradiction.

[0114] The above description is only a preferred embodiment of the present invention, and does not limit the patent scope of the present invention. All equivalent structural changes made by using the contents of the present invention specification and drawings under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields are included in the patent protection scope of the present invention.

Claims

1. A preimplantation genetic diagnosis embryo biopsy device, characterized in that: include: An incubation platform, wherein a plurality of incubation stations are arranged on the incubation platform, each of which has an incubation tank suitable for placing a culture dish and a sampling tank suitable for inserting a sampling tube; the plurality of incubation stations are circumferentially spaced around an axis; A transparent cover, the transparent cover is located above the incubation platform, and the transparent cover is provided with an opening suitable for revealing a culture station; A rotating seat, which is disposed at the bottom of the incubation table and is used to drive the incubation table to rotate relative to the transparent cover so as to selectively switch one of the culture stations to the opening; The rotating seat comprises a base, a rotating disk, a worm wheel, a worm and a driving motor. The rotating disk is pivotally arranged on the base around its own axis, and the incubation table is fixed on the rotating disk and can rotate with the rotating disk; the worm wheel is arranged at the bottom of the rotating disk and is coaxial with the rotating disk, the worm is arranged in the base and can pivot around its own axis, and the worm is meshed with the worm wheel; the driving motor is connected to the worm to drive the worm to rotate; The top surface of the base is provided with a receiving cavity, the bottom of the receiving cavity is provided with a cylindrical boss, the bottom of the rotating disk is provided with a sleeve portion, and the sleeve portion is sleeved on the cylindrical boss so that the rotating disk can pivot around its own axis relative to the base.

2. The preimplantation genetic diagnosis embryo biopsy device according to claim 1, characterized in that: A through hole is provided at the center of the incubation table, and a positioning piece is provided on the cylindrical boss; A downwardly protruding connecting column is provided at the bottom of the transparent cover, a positioning hole is provided at the bottom of the connecting column, the connecting column passes through the through hole, and the positioning hole is sleeved with the positioning piece to fix the transparent cover and the base relatively in the circumferential direction.

3. The preimplantation genetic diagnosis embryo biopsy device according to claim 2, characterized in that: The incubation platform comprises: A base, the base is fixed on the rotating base, the base is provided with the through hole, a plurality of the sampling grooves surrounding the outside of the through hole, and an annular groove surrounding the outside of the plurality of the sampling grooves, and the plurality of the sampling grooves are arranged at equal intervals; A heating film, the heating film is formed in an annular shape and is arranged in the annular groove; A heat conducting plate is formed in a ring shape and is attached above the heating film. A plurality of the incubation grooves are arranged on the heat conducting plate. The plurality of the incubation grooves are arranged at equal intervals and correspond one-to-one to the plurality of the sampling grooves.

4. The preimplantation genetic diagnosis embryo biopsy device according to claim 3, characterized in that: The base comprises: A cylindrical portion, wherein the through hole is arranged at the center of the cylindrical portion, and a plurality of sampling slots are arranged on the top surface of the cylindrical portion and surround the through hole; The annular portion is formed by protruding radially outward from the upper end of the cylindrical portion, and the annular groove is arranged on the top surface of the annular portion.

5. The preimplantation genetic diagnosis embryo biopsy device according to claim 4, characterized in that: The upper end of the cylindrical portion protrudes radially toward the annular groove to form a positioning portion, and the inner edge of the heat conducting plate is provided with a positioning opening matched with the positioning portion.

6. The preimplantation genetic diagnosis embryo biopsy device according to claim 3, characterized in that: The outer circumferential surface of the rotating disk is provided with a plurality of optical coupling baffles at intervals, and the plurality of optical coupling baffles correspond one-to-one to the plurality of the culture stations; The base is provided with an upwardly extending bracket, and the bracket is provided with a photoelectric switch. Each time the rotating disk rotates a predetermined angle, one of the plurality of optical coupling baffles rotates in rotation into the photoelectric switch, so that the photoelectric switch generates a trigger signal, and the trigger signal is used to control the drive motor to stop, so that one of the plurality of culture stations switches to the opening.

7. The preimplantation genetic diagnosis embryo biopsy device according to claim 3, characterized in that: The upper end of the accommodating cavity is open to form an opening, and the opening is provided with a lip edge protruding radially inward, and the lip edge is provided with an elastic power supply contact connected to the power source; A conductive ring is provided on the bottom surface of the rotating disk, and the conductive ring is in electrical contact with the elastic power supply contact. The electrode of the heating film is provided at the bottom of the base and is electrically connected to the conductive ring.

8. The preimplantation genetic diagnosis embryo biopsy device according to claim 2, characterized in that: The cross section of the positioning hole is T-shaped, and the cross section shape of the positioning piece is matched with the positioning hole.

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