Nucleic acid detection kit

Abstract

The present invention relates to a nucleic acid detection kit, comprising: a main shell, having a plurality of accommodating chambers and at least one plunger cavity with an opening at one end, the main shell also having a liquid injection hole and an exhaust hole respectively connected to the plunger cavity; a plunger assembly, detachably inserted in the plunger cavity; a reaction chamber, detachably installed in the main shell, the reaction chamber having a detection chamber and a liquid injection channel and an exhaust channel respectively connected to the detection chamber, when the reaction chamber is connected to the main shell, the liquid injection channel can be selectively connected to the liquid injection hole, and the exhaust channel can be selectively connected to the exhaust hole; wherein, when the plunger assembly is inserted into the plunger cavity, the plunger assembly and the plunger cavity jointly form a positive pressure that causes the liquid in the plunger cavity to flow into the liquid injection channel through the liquid injection hole. In the above nucleic acid detection kit, the nucleic acid sample can be injected into the reaction chamber without centrifugation, thereby simplifying the nucleic acid extraction step and improving the nucleic acid extraction efficiency.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, in particular to a nucleic acid detection kit. Background Art

[0002] Nucleic acid is the carrier of genetic information of living organisms and is an essential component of all known life forms. There are two types of nucleic acids, DNA and RNA, which mainly exist in the cell nucleus and exist in a state of binding to proteins. With the rapid development of molecular biology, the research and analysis of nucleic acids has been continuously promoted and applied in the fields of clinical diagnosis, food safety, environmental testing, agriculture, forestry and animal husbandry.

[0003] Polymerase Chain Reaction (PCR) technology is a technique for rapid in vitro DNA amplification. This technology can be used in basic research such as gene isolation, cloning and nucleic acid sequence analysis. In addition, it can also be used in any other place containing DNA or RNA, such as in the process of disease diagnosis.

[0004] However, the current PCR reaction tube and reagent strip are two independent parts. After completing the nucleic acid purification process in the reagent strip, the nucleic acid sample needs to be added to the PCR reaction tube, which is manually covered with a sealing cap and placed in a centrifuge for high-speed centrifugation before the nucleic acid sample can be injected into the PCR detection chamber. Therefore, the operation process is relatively complicated and the operating efficiency is low. Summary of the invention

[0005] Based on this, it is necessary to provide a nucleic acid detection kit to address the problem of complex nucleic acid extraction operations, which can achieve the technical effect of simplifying nucleic acid extraction operations.

[0006] According to one aspect of the present application, a nucleic acid detection kit is provided, comprising:

[0007] A main housing having a plurality of accommodating chambers and at least one plunger cavity with an opening at one end, and the main housing also has a liquid injection hole and an exhaust hole respectively connected to the plunger cavity;

[0008] a plunger assembly, detachably inserted into the plunger cavity from the open end of the plunger cavity; and

[0009] A reaction chamber is detachably mounted on the main housing, wherein the reaction chamber has a detection chamber and a liquid injection channel and an exhaust channel respectively connected to the detection chamber, and when the reaction chamber is matched with the main housing, the liquid injection channel can be selectively connected to the liquid injection hole, and the exhaust channel can be selectively connected to the exhaust hole;

[0010] Wherein, when the plunger assembly is inserted into the plunger cavity, the plunger assembly and the plunger cavity together form a positive pressure that causes the liquid in the plunger cavity to flow into the injection channel through the injection hole.

[0011] In one embodiment, when the plunger assembly is inserted into the plunger cavity, the plunger assembly switches from the first state to the second state;

[0012] When the plunger assembly is in the first state, the injection hole and the exhaust hole are both connected to the plunger cavity;

[0013] When the plunger assembly is in the second state, the plunger assembly closes the liquid injection hole and the exhaust hole.

[0014] In one embodiment, the distance between the injection hole and the opening end of the plunger cavity is greater than the distance between the exhaust hole and the opening end of the plunger cavity.

[0015] In one embodiment, the plunger cavity includes a first cavity and a second cavity sequentially arranged along the insertion direction of the plunger assembly, the inner diameter of the first cavity is larger than the inner diameter of the second cavity, the exhaust hole is connected to the first cavity, and the injection hole is connected to the second cavity; the plunger assembly includes a first sealing section and a second sealing section connected to each other, and the outer diameter of the first sealing section is larger than the outer diameter of the second sealing section;

[0016] When the plunger assembly is in the first state, the first sealing section has a clearance fit with one end of the first cavity away from the second cavity; when the plunger assembly is in the second state, the first sealing section has an interference fit with one end of the first cavity away from the second cavity, and the end of the second sealing section away from the first sealing section has an interference fit with the second cavity.

[0017] In one embodiment, the plunger assembly includes a plunger body and a plunger covering body, wherein the plunger body includes a connecting portion and a sealing portion connected to each other;

[0018] The plunger covering body covers the outside of the sealing portion to form the first sealing section and the second sealing section. The plunger covering body can generate a recoverable deformation under the action of an external force to achieve an interference fit with the plunger cavity.

[0019] In one embodiment, the nucleic acid detection kit further includes a first seal and a second seal;

[0020] The first sealing member is disposed at the inlet end of the injection channel, and the first sealing member can be opened or closed according to the external pressure to connect or disconnect the injection channel and the injection hole;

[0021] The second sealing member is disposed at the outlet end of the exhaust passage, and the second sealing member can be opened or closed according to the external pressure to connect or disconnect the exhaust passage and the exhaust hole.

[0022] In one embodiment, the main shell is provided with a snap-in hole; the reaction chamber includes a reaction chamber body and a reaction chamber clamping arm, the detection chamber is arranged in the reaction chamber body, one end of the reaction chamber clamping arm is connected to one side of the reaction chamber body, and the other end of the reaction chamber clamping arm can be detachably clamped in the snap-in hole.

[0023] In one embodiment, the reaction chamber body is injection molded from a transparent material.

[0024] In one embodiment, a plurality of the containing chambers and the plunger chambers are spaced apart and arranged around an axis.

[0025] In one embodiment, a plurality of the containing bins and the plunger cavity are arranged in a straight line at intervals, and the plunger cavity is located on the same side of all the containing bins.

[0026] In the above-mentioned nucleic acid detection kit, the reaction chamber can be detachably mounted on the main housing, and the nucleic acid detection kit can cooperate with a pipette to process the sample to be tested in multiple containing chambers to obtain the required nucleic acid sample, and then inject the nucleic acid sample into the plunger cavity through the pipette head, and then press the nucleic acid sample into the reaction chamber through the plunger assembly. In the process of pressing the nucleic acid sample into the reaction chamber, the gas in the reaction chamber can be discharged through the exhaust hole, so that the nucleic acid sample can smoothly enter the reaction chamber. Therefore, the process of the plunger assembly pressing the nucleic acid sample into the reaction chamber replaces the centrifugation process in the prior art, so that the nucleic acid sample can be smoothly injected into the reaction chamber without centrifugation, thereby simplifying the nucleic acid extraction step and improving the nucleic acid extraction efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 This is a schematic diagram of the structure of a nucleic acid detection kit according to an embodiment of the present invention;

[0028] Figure 2 for Figure 1 A schematic diagram of the decomposition of the nucleic acid detection kit shown;

[0029] Figure 3 for Figure 1 A cross-sectional view of the main shell of the nucleic acid detection kit shown;

[0030] Figure 4 for Figure 1 A cross-sectional view of the plunger assembly of the nucleic acid detection kit shown;

[0031] Figure 5 for Figure 1A cross-sectional view of a reaction chamber of the nucleic acid detection kit shown;

[0032] Figure 6 for Figure 1 A schematic diagram of the plunger assembly of the nucleic acid detection kit shown in the first state;

[0033] Figure 7 for Figure 6 A partial enlarged view of the middle A;

[0034] Figure 8 for Figure 1 A schematic diagram of the plunger assembly of the nucleic acid detection kit shown in the second state;

[0035] Fig. 9 for Figure 8 A partial enlarged view of point B in the middle;

[0036] Fig.10 This is a schematic structural diagram of a nucleic acid detection kit according to another embodiment of the present invention;

[0037] Fig.11 for Fig.10 A schematic diagram of the decomposition of the nucleic acid detection kit shown;

[0038] Fig.12 for Fig.10 A cross-sectional view of the nucleic acid detection kit shown.

[0039] Description of Figure Numbers:

[0040] 100. Nucleic acid detection kit; 10. Main shell; 11. Shell bottom wall; 13. Shell top wall; 15. Shell side wall; 152. Liquid injection hole; 154. Exhaust hole; 17. Accommodation chamber; 19. Plunger cavity; 192. First cavity; 194. Second cavity; 20. Plunger assembly; 21. Plunger body; 212. Connecting part; 214. Sealing part; 23. Plunger covering body; 232. First sealing section; 234. Second sealing section; 30. Pipette tip; 40. Reaction chamber; 41. Reaction chamber body; 412. Detection chamber; 414. Liquid injection channel; 416. Exhaust channel; 43. Reaction chamber holding arm; 50. First sealing member; 60. Second sealing member. DETAILED DESCRIPTION

[0041] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific embodiments disclosed below.

[0042] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as limiting the present invention.

[0043] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In the description of the present invention, the meaning of "plurality" is at least two, such as two, three, etc., unless otherwise clearly and specifically defined.

[0044] In the present invention, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0045] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0046] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "upper", "lower", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0047] like Figure 1 and Figure 2 As shown, an embodiment of the present invention provides a nucleic acid detection kit 100, through which an operator can extract nucleic acid from a sample. The nucleic acid detection kit 100 includes a main housing 10, a plunger assembly 20, a pipette tip 30, and a reaction chamber 40. The main housing 10 has a plurality of receiving chambers 17 with one end open and at least one plunger cavity 19 with one end open. Part of the receiving chamber 17 is used to store reagents, and part of the receiving chamber 17 is used to accommodate the plunger assembly 20 and the pipette tip 30. The reaction chamber 40 is detachably mounted on the main housing 10.

[0048] In the above-mentioned nucleic acid detection kit 100, the reaction chamber 40 is detachably mounted on the main housing 10. The nucleic acid detection kit 100 can cooperate with a pipette to process the sample to be tested in a plurality of containing chambers 17 to obtain the required nucleic acid sample, and then inject the nucleic acid sample into the plunger cavity 19 through the pipette head 30, and then press the nucleic acid sample into the reaction chamber 40 through the plunger assembly 20. In the process of pressing the nucleic acid sample into the reaction chamber 40, the gas in the reaction chamber 10 can be discharged at the same time, so that the nucleic acid sample can smoothly enter the reaction chamber 40. Therefore, the process of the plunger assembly 20 pressing the nucleic acid sample into the reaction chamber 40 replaces the centrifugation process in the prior art, so that the nucleic acid sample can be smoothly injected into the reaction chamber 40 without centrifugation, thereby simplifying the nucleic acid extraction step and improving the nucleic acid extraction efficiency.

[0049] Specifically, in some embodiments, the main housing 10 is in a long strip structure, and a plurality of accommodating chambers 17 and plunger cavities 19 are sequentially arranged in intervals along the length direction of the main housing 10, and the plunger cavity 19 is provided at one end in the length direction of the main housing 10. The following embodiments take the main housing 10 in a long strip structure as an example to describe the specific structure of the nucleic acid detection kit 100.

[0050] Please continue reading Figure 1 and Figure 2 The main housing 10 has a housing bottom wall 11, a housing top wall 13 and a housing side wall 15. The housing bottom wall 11 and the housing top wall 13 are arranged along a first direction (i.e. Figure 1The housing side wall 15 is connected between the housing bottom wall 11 and the housing top wall 13. One end of each accommodating bin 17 and the plunger cavity 19 is connected to the housing top wall 13, and the other end of the accommodating bin 17 and the plunger cavity 19 extends from the housing top wall 13 toward the housing bottom wall 11 along the first direction.

[0051] Combination Figure 3 As shown, the plunger cavity 19 includes a first cavity 192 and a second cavity 194 which are sequentially arranged along a first direction. One end of the first cavity 192 is connected to the housing top wall 13 to form an open end of the plunger cavity 19, and the other end of the first cavity 192 extends from the housing top wall 13 toward the housing bottom wall 11, and one end of the second cavity 194 is connected to one end of the first cavity 192 toward the housing bottom wall 11, and the other end of the second cavity 194 extends toward the housing bottom wall 11, and the inner diameter of the first cavity 192 is greater than the inner diameter of the second cavity 194.

[0052] The housing side wall 15 at one end of the main housing 10 provided with the plunger cavity 19 is provided with a liquid injection hole 152 and a gas exhaust hole 154, the liquid injection hole 152 and the gas exhaust hole 154 are arranged at intervals in the first direction, and the distance between the liquid injection hole 152 and the open end of the plunger cavity 19 is greater than the distance between the gas exhaust hole 154 and the open end of the plunger cavity 19. One end of the liquid injection hole 152 is connected to the outer surface of the housing side wall 15, and the other end of the liquid injection hole 152 extends along the length direction of the main housing 10 until it is connected to the plunger cavity 19; one end of the gas exhaust hole 154 is connected to the outer surface of the housing side wall 15, and the other end of the gas exhaust hole 154 extends along the length direction of the main housing 10 until it is connected to the plunger cavity 19. Preferably, the liquid injection hole 152 is connected to the bottom of the second cavity 194 away from the housing bottom wall 11, and the gas exhaust hole 154 is connected to one end of the first cavity 192 close to the housing top wall 13.

[0053] In this way, the reaction chamber 40 can be connected to the upper and lower ends of the plunger cavity 19 in the first direction through the injection hole 152 and the exhaust hole 154 respectively. The nucleic acid sample in the plunger cavity 19 can flow into the reaction chamber 40 through the injection hole 152, and the gas in the reaction chamber 40 can also enter the plunger cavity 19 through the exhaust hole 154.

[0054] like Figure 4As shown, the plunger assembly 20 includes a plunger body 21 and a plunger coating 23, and the plunger body 21 includes a connecting portion 212 and a sealing portion 214 that are connected to each other. The connecting portion 212 is used to connect to an external driving device, and the external driving device can drive the plunger assembly 20 to move through the connecting portion 212. The plunger coating 23 is coated outside the sealing portion 214, and the plunger coating 23 can produce a recoverable deformation under the action of an external force to interfere with the plunger cavity 19. Specifically, in some embodiments, the plunger body 21 is formed of a hard rubber material with a relatively high hardness, so that it has good support, and the plunger coating 23 is formed of a soft rubber material with a relatively low hardness, so that it can be deformed to a certain extent to interfere with the plunger cavity 19, thereby ensuring the sealing of the plunger assembly 20.

[0055] Furthermore, the plunger covering body 23 forms a first sealing segment 232 and a second sealing segment 234. The first sealing segment 232 covers the sealing portion 214 and is connected to one end of the connecting portion 212. The outer diameter of the first sealing segment 232 is greater than the outer diameter of the second sealing segment 234 and the inner diameter of the first cavity 192. The length of the first sealing segment 232 in the first direction is less than the length of the first cavity 192 in the first direction. The diameter of the second sealing segment 234 is greater than the inner diameter of the second cavity 194. The length of the second sealing segment 234 in the first direction is greater than the length of the second cavity 194 in the second direction.

[0056] See also Figures 6 to 9 The plunger assembly 20 can be inserted into the plunger cavity 19 along the first direction to form a positive pressure with the plunger cavity 19 to make the liquid in the plunger cavity 19 flow into the injection channel 414 through the injection hole 152, thereby replacing the centrifugal process in the prior art.

[0057] Specifically, when the plunger assembly 20 is inserted into the plunger cavity 19, the plunger assembly 20 switches from the first state to the second state. When the plunger assembly 20 is in the first state, the first sealing section 232 of the plunger assembly 20 is gap-fitted with one end of the first cavity 192 away from the second cavity 194 and is spaced apart at the side of the exhaust hole 154 facing the top wall 13 of the housing, and the second sealing section 234 of the plunger assembly 20 is spaced apart at the side of the injection hole 152 facing the top wall 13 of the housing and is interference-fitted with the second cavity 194, so that the injection hole 152 and the exhaust hole 154 are in an exposed state and communicated with the plunger cavity 19, the liquid in the plunger cavity 19 can flow into the injection hole 152, and the gas in the exhaust hole 154 can also enter the plunger cavity 19, and then flow to the external environment through the gap between the first sealing section 232 and the first cavity 192. When the plunger assembly 20 is in the second state, the first sealing section 232 is interference fit with the end of the first cavity 192 away from the second cavity 194 and closes the exhaust hole 154, and the end of the second sealing section 234 away from the first sealing section 232 is interference fit with the second cavity 194 and closes the injection hole 152, so that no liquid flows into the injection hole 152 and no gas flows out of the exhaust hole 154.

[0058] like Figure 5 As shown, the reaction chamber 40 is detachably inserted in a direction perpendicular to the first direction on a side of the main housing 10 provided with a plunger cavity 19 to communicate with the plunger cavity 19. Specifically, the main housing 10 is provided with two spaced-apart clamping holes, and the reaction chamber 40 includes a reaction chamber body 41 and two reaction chamber clamping arms 43. The reaction chamber body 41 is a hollow flat shell structure, and has a detection cavity 412 and a liquid injection channel 414 and an exhaust channel 416 communicating with the detection cavity 412. One end of each reaction chamber clamping arm 43 is connected to the reaction chamber body 41, and the other end of the reaction chamber clamping arm 43 is detachably clamped in the clamping hole of the main housing 10. When the reaction chamber 40 is connected to the main shell 10 through the reaction chamber clamping arm 43, the injection channel 414 is connected to the injection hole 152, and the exhaust channel 416 is connected to the exhaust hole 154. The liquid in the injection hole 152 can flow into the detection chamber 412 through the injection channel 414, and the gas in the detection chamber 412 can be discharged into the plunger chamber 19 through the exhaust channel 416 and the exhaust hole 154.

[0059] In some embodiments, the nucleic acid detection kit 100 also includes a first seal 50 and a second seal 60. The first seal 50 is disposed at the inlet end of the injection channel 414. The first seal 50 can be opened or closed according to the external pressure to connect or disconnect the injection channel 414 and the injection hole 152. The second seal 60 is disposed at the outlet end of the exhaust channel 416. The second seal 60 can be opened or closed according to the external pressure to connect or disconnect the exhaust channel 416 and the exhaust hole 154.

[0060] Please refer again Figures 6 to 9 Specifically, when the plunger assembly 20 switches from the first state to the second state along the first direction, the positive pressure formed by the plunger assembly 20 and the liquid storage chamber forces the first seal 50 to open, so the liquid in the plunger chamber 19 enters the injection channel 414 through the first seal 50, and the pressure in the detection chamber 412 increases accordingly, forcing the second seal 60 to open, so the gas in the detection chamber 412 enters the exhaust channel 416 through the second seal 60. When the plunger assembly 20 switches to the second state, the positive pressure applied to the first seal 50 disappears, and the first seal 50 and the second seal 60 return to the closed state. In this way, the injection channel 414 can be selectively connected to the injection hole 152, and the exhaust channel 416 can be selectively connected to the exhaust hole 154, so as to ensure the sealing of the detection chamber 412 and avoid leakage of the liquid in the detection chamber 412.

[0061] In some embodiments, the reaction chamber body 41 is injection molded from a transparent material, so that the operator can easily observe the amount of liquid in the reaction chamber 40. It can be understood that the material and processing method of forming the reaction chamber body 41 are not limited and can be set as needed to meet different needs.

[0062] The basic principle of nucleic acid extraction using the above nucleic acid detection kit 100 is as follows:

[0063] First, the samples to be tested are processed in a plurality of containing chambers 17 to obtain the desired nucleic acid samples.

[0064] Then, the reaction chamber 40 is installed on the main housing 10 , the injection channel 414 of the reaction chamber 40 is connected to the injection hole 152 , and the exhaust channel 416 of the reaction chamber 40 is connected to the exhaust hole 154 .

[0065] Afterwards, the nucleic acid sample is introduced into the plunger cavity 19 using the pipette tip 30 , and then the plunger assembly 20 is inserted into the plunger cavity 19 along the first direction using an external driving device.

[0066] As the plunger assembly 20 moves, the plunger assembly 20 is first in the first state, the second sealing section 234 of the plunger assembly 20 blocks one end of the second cavity 194 connected to the first cavity 192, the first sealing member 50 and the second sealing member 60 are opened under pressure, and the nucleic acid sample in the plunger cavity 19 enters the detection cavity 412 of the reaction chamber 40 through the injection hole 152 and the injection channel 414, and the gas in the detection cavity 412 is discharged into the plunger cavity 19 through the exhaust channel 416 and the exhaust hole 154.

[0067] As the plunger assembly 20 continues to move, the plunger assembly 20 switches to the second state, the first sealing segment 232 closes the exhaust hole 154, and the second sealing segment 234 closes the injection hole 152, so there is no flow of liquid or gas in the injection channel 414 and the exhaust channel 416, so the first seal 50 and the second seal 60 return to the closed state.

[0068] Finally, the reaction chamber 40 is removed from the main housing 10, and the plunger assembly 20 is taken out of the plunger cavity 19 by an external driving device.

[0069] See also Figures 10 to 12 In some other embodiments, the main shell 10 has a columnar structure, and a plurality of accommodating chambers 17 and plunger chambers 19 are arranged at intervals around the central axis of the main shell 10 . The structure of the plunger chamber 19 is the same as that in the above-mentioned embodiment, so that the nucleic acid sample in the plunger chamber 19 can also be smoothly injected into the reaction chamber 40.

[0070] In the above-mentioned nucleic acid detection kit 100, the nucleic acid sample is pressurized and injected into the reaction chamber 40 by the plunger assembly 20, thereby eliminating the centrifugation process in the prior art, thereby simplifying the nucleic acid extraction process, saving experimental costs, and improving experimental efficiency. Moreover, the entire nucleic acid extraction process can be completed in the nucleic acid detection kit 100, optimizing the user experience of the nucleic acid detection kit 100.

[0071] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0072] The above-mentioned embodiments only express several implementation methods of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the scope of the invention patent. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A nucleic acid detection kit, characterized in that: include: A main housing having a plurality of accommodating chambers and at least one plunger cavity with an opening at one end, and the main housing also has a liquid injection hole and an exhaust hole respectively connected to the plunger cavity; A plunger assembly is detachably inserted into the plunger cavity from the open end of the plunger cavity; A reaction chamber is detachably mounted on the main housing, wherein the reaction chamber has a detection chamber and a liquid injection channel and an exhaust channel respectively connected to the detection chamber, and when the reaction chamber is matched with the main housing, the liquid injection channel can be selectively connected to the liquid injection hole, and the exhaust channel can be selectively connected to the exhaust hole; as well as A pipette tip, housed in the containing chamber; Wherein, when the plunger assembly is inserted into the plunger cavity, the plunger assembly and the plunger cavity together form a positive pressure that causes the liquid in the plunger cavity to flow into the injection channel through the injection hole.

2. The nucleic acid detection kit according to claim 1, characterized in that: When the plunger assembly is inserted into the plunger cavity, the plunger assembly switches from the first state to the second state; When the plunger assembly is in the first state, the injection hole and the exhaust hole are both connected to the plunger cavity; When the plunger assembly is in the second state, the plunger assembly closes the liquid injection hole and the exhaust hole.

3. The nucleic acid detection kit according to claim 2, characterized in that: The distance between the injection hole and the opening end of the plunger cavity is greater than the distance between the exhaust hole and the opening end of the plunger cavity.

4. The nucleic acid detection kit according to claim 2, characterized in that: The plunger cavity comprises a first cavity and a second cavity sequentially arranged along the insertion direction of the plunger assembly, the inner diameter of the first cavity is larger than the inner diameter of the second cavity, the exhaust hole is connected to the first cavity, and the injection hole is connected to the second cavity; the plunger assembly comprises a first sealing section and a second sealing section connected to each other, and the outer diameter of the first sealing section is larger than the outer diameter of the second sealing section; When the plunger assembly is in the first state, the first sealing section has a clearance fit with one end of the first cavity away from the second cavity; when the plunger assembly is in the second state, the first sealing section has an interference fit with one end of the first cavity away from the second cavity, and the end of the second sealing section away from the first sealing section has an interference fit with the second cavity.

5. The nucleic acid detection kit according to claim 4, characterized in that: The plunger assembly comprises a plunger body and a plunger covering body, wherein the plunger body comprises a connecting portion and a sealing portion connected to each other; The plunger covering body covers the outside of the sealing portion to form the first sealing section and the second sealing section. The plunger covering body can generate a recoverable deformation under the action of an external force to achieve an interference fit with the plunger cavity.

6. The nucleic acid detection kit according to claim 1, characterized in that: The nucleic acid detection kit also includes a first sealing member and a second sealing member; The first sealing member is disposed at the inlet end of the injection channel, and the first sealing member can be opened or closed according to the external pressure to connect or disconnect the injection channel and the injection hole; The second sealing member is disposed at the outlet end of the exhaust passage, and the second sealing member can be opened or closed according to the external pressure to connect or disconnect the exhaust passage and the exhaust hole.

7. The nucleic acid detection kit according to claim 1, characterized in that: The main shell is provided with a snap-in hole; the reaction chamber includes a reaction chamber body and a reaction chamber clamping arm, the detection chamber is arranged in the reaction chamber body, one end of the reaction chamber clamping arm is connected to one side of the reaction chamber body, and the other end of the reaction chamber clamping arm is detachably clamped in the snap-in hole.

8. The nucleic acid detection kit according to claim 1, characterized in that: The reaction chamber body is injection-molded from a transparent material.

9. The nucleic acid detection kit according to any one of claims 1 to 8, characterized in that: The plurality of accommodating chambers and the plunger chambers are spaced apart and arranged around an axis.

10. The nucleic acid detection kit according to any one of claims 1 to 8, characterized in that: The plurality of accommodating chambers and the plunger chambers are arranged at intervals along a straight line.

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