A round-leaved sicklepod decomposition microbial inoculum, its preparation method and application

Abstract

The present invention relates to the technical field of microbial decomposition, and particularly relates to a Corchorus rotundus decomposing microbial inoculum, a preparation method and an application thereof. The decomposing microbial inoculum comprises Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, white rot fungi, Aspergillus tubingensis, Pseudomonas and an activator. The activator comprises raw materials in the following parts by weight: 40-60 parts of apatite powder, 15-20 parts of glucose, 5-10 parts of minerals and 5-10 parts of citric acid. The decomposing microbial inoculum disclosed by the present invention has a high decomposition rate for Corchorus rotundus, and can significantly improve the decomposition rates of total carbon, total nitrogen and total phosphorus of Corchorus rotundus.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial decomposition, and specifically relates to a chamaecrista rotundifolia decomposition microbial inoculum, a preparation method thereof and an application thereof. Background Art

[0002] Chamaecrista rotundifolia is a leguminous green manure native to North America, Central America and northern South America. After being introduced into China in the 1980s, it has strong adaptability in southern China. Research shows that chamaecrista rotundifolia has characteristics such as acid tolerance, barren tolerance, strong resistance, rapid growth and the ability to germinate and grow spontaneously.

[0003] Fruit trees are an important economic crop. In recent years, the area of orchards has been continuously expanding. In order to pursue higher economic benefits, fruit farmers have long adopted a clean tillage mode and applied a large amount of chemical fertilizers during the process of growing fruit trees. The change in the tillage mode and the large application of chemical fertilizers have changed the nutrient status, microbial community structure and activity of the soil, etc., which have an adverse impact on the growth of fruit trees and the soil environment.

[0004] After being turned under as green manure, chamaecrista rotundifolia will bring new organic substances to the soil, and these organic substances provide organic nutrients for the reproduction and activities of soil bacteria. However, the low natural decomposition rate after chamaecrista rotundifolia is turned under results in the inability to provide nutrients for the growth of fruit trees in a timely manner, affecting the yield and quality of fruit trees. At present, there is no chamaecrista rotundifolia decomposition microbial inoculum on the market. Summary of the Invention

[0005] In order to solve the technical problem of the low decomposition rate of chamaecrista rotundifolia after being turned under as green manure, the present invention discloses a chamaecrista rotundifolia decomposition microbial inoculum, a preparation method thereof and an application thereof.

[0006] A Corchorus rot-decomposing microbial agent, the rot-decomposing microbial agent comprising Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis, Pseudomonas and an activator; the preservation number of Aspergillus niger (Aspergillus niger van Tieghem) is GDMCC NO. 3.250; the preservation number of Trichothecium roseum is GDMCC NO. 3.500; the preservation number of Trichoderma longibrachiatum is GDMCC NO. 3.140; the preservation number of Bacillus subtilis is GDMCC NO. 1.1665; the preservation number of Phanerochaete chrysosporium is GDMCC NO. 3.383; the preservation number of Aspergillus tubingensis is GDMCC NO. 3.577; the preservation number of Pseudomonas is GDMCC NO. 1.462; the above-mentioned microbial strains can all be purchased from the Guangdong Provincial Center for Microbial Strains.

[0007] Preferably, for a Corchorus rot-decomposing microbial agent, the microbial contents in the rot-decomposing microbial agent are as follows: Aspergillus niger 0.5 - 0.7×10 9 cfu / g, Trichothecium roseum 0.6 - 0.8×10 9 cfu / g, Trichoderma longibrachiatum 1.2 - 1.5×10 9 cfu / g, Bacillus subtilis 1.0 - 1.3×10 9 cfu / g, Phanerochaete chrysosporium 1.2 - 1.4×10 9 cfu / g, Aspergillus tubingensis 0.9 - 1.2×10 9 cfu / g, Pseudomonas 1.1 - 1.3×10 9 cfu / g; the activator comprises raw materials in the following parts by weight: 40 - 60 parts of apatite powder, 15 - 20 parts of glucose, 5 - 10 parts of minerals, 5 - 10 parts of citric acid.

[0008] Preferably, the mass ratio of the microorganisms to the activator is 20 - 35:1 - 3.

[0009] Preferably, the apatite powder is hydroxyapatite powder with a particle size of 50 - 100 μm.

[0010] The preparation method of the above-mentioned Corchorus rot-decomposing microbial agent is prepared by the following steps:

[0011] (1) Preparation of activator: Weigh citric acid, minerals and glucose by weight parts, add water with a mass 5 - 15 times of theirs, after complete dissolution, add hydroxyapatite powder, then add it into a reaction kettle, stir, the temperature in the reaction kettle is 40 - 50 °C, the pressure is 1.2 - 1.5 MPa, after stirring for 30 - 60 min, carry out vacuum drying to obtain the activator;

[0012] (2) Mixing the activator and microorganisms: Add Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis, Pseudomonas and the activator into a reaction kettle, the temperature in the reaction kettle is 20 - 35 °C, the pressure is 1.3 - 1.7 MPa, the stirring time is 1 - 1.5 h, stir evenly, carry out vacuum drying, then the Corchorus rotundus decomposing microbial agent is obtained.

[0013] Application of the said decomposing microbial agent to decompose Corchorus rotundus in red - soil dry - land orchard soil, the specific application method is as follows:

[0014] (1) Pretreatment: Wash the sundries adhered to the surface of Corchorus rotundus, dry it for later use;

[0015] (2) Soaking: Put Corchorus rotundus into the bacterial water containing the decomposing microbial agent and soak for 5 - 8 min;

[0016] (3) Decomposing: Turn and press the soaked Corchorus rotundus into the soil.

[0017] Preferably, add nitrogen - fixing bacteria to the decomposing microbial agent in the said step (2), the mass ratio of the decomposing microbial agent to the nitrogen - fixing bacteria is 45 - 60:1 - 4.

[0018] Preferably, add high - carbon - based soil remediation organic fertilizer in the said step (3).

[0019] Preferably, add high - carbon - based soil remediation organic fertilizer in the said step (3), the mass ratio of the high - carbon - based soil remediation organic fertilizer to the dry weight of Corchorus rotundus is 1 - 3:3 - 5.

[0020] The present invention discloses a Corchorus rotundus decomposing microbial agent, its preparation method and its application. Using the decomposing microbial agent made of Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis, Pseudomonas and the activator to treat Corchorus rotundus can significantly improve the decomposition rates of plant biomass, total carbon, total nitrogen and total phosphorus; in addition, the combined use of high - carbon - based soil remediation organic fertilizer and the decomposing microbial agent will have a better effect than using the decomposing microbial agent alone; while the combined use of nitrogen - fixing bacteria and the decomposing microbial agent has an effect between that of high - carbon - based soil remediation organic fertilizer + decomposing microbial agent and using the decomposing microbial agent alone. The high - carbon - based soil remediation organic fertilizer contains natural minerals, humic acid and trace element components, which enhance the microbial activity in the decomposing microbial agent and promote the decomposition of Corchorus rotundus; as a symbiotic bacterium of leguminous plants, adding nitrogen - fixing bacteria to the decomposing microbial agent can also promote the decomposition of Corchorus rotundus. Description of the Drawings

[0021] Figure 1 It is a graph of the biomass change of Chamaecrista rotundifolia plants.

[0022] Figure 2 It is a graph of the cumulative decomposition rate of the biomass of Chamaecrista rotundifolia.

[0023] Figure 3 It is a graph of the change in the total carbon of Chamaecrista rotundifolia.

[0024] Figure 4 It is a graph of the cumulative decomposition rate of the carbon of Chamaecrista rotundifolia.

[0025] Figure 5 It is a graph of the change in the total nitrogen of Chamaecrista rotundifolia.

[0026] Figure 6 It is a graph of the cumulative decomposition rate of the nitrogen of Chamaecrista rotundifolia.

[0027] Figure 7 It is a graph of the change in the total phosphorus of Chamaecrista rotundifolia.

[0028] Figure 8 It is a graph of the cumulative decomposition rate of the phosphorus of Chamaecrista rotundifolia.

[0029] Figure 9 It is a graph of the change in the total potassium of Chamaecrista rotundifolia.

[0030] Figure 10 It is a graph of the cumulative decomposition rate of the potassium of Chamaecrista rotundifolia. Detailed implementation mode

[0031] To make the objectives, technical solutions and advantages of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, rather than all of the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0032] Example 1

[0033] A kind of decomposition bacteria agent for Chamaecrista rotundifolia, the microbial contents in the decomposition bacteria agent are respectively: Aspergillus niger 0.5×10 9 cfu / g, Trichothecium roseum 0.6 - ×10 9 cfu / g, Trichoderma longibrachiatum 1.2×10 9 cfu / g, Bacillus subtilis 1.0×10 9 cfu / g, Phanerochaete chrysosporium 1.2×10 9 cfu / g, Aspergillus tubingensis 0.9×10 9 cfu / g, Pseudomonas sp. 1.1×10 9cfu / g; The activator comprises raw materials in the following parts by weight: 40 parts of apatite powder, 15 parts of glucose, 5 parts of minerals, and 5 parts of citric acid; the particle size of the hydroxyapatite powder is 50 μm; the mass ratio of the microorganism to the activator is 20:1.

[0034] A method for preparing a decomposing agent of Chamaecrista rotundifolia, the specific steps are as follows:

[0035] (1) Prepare the activator: Weigh citric acid, minerals and glucose by parts by weight, add water 5 times their mass, after complete dissolution, add hydroxyapatite powder, then add it to a reaction kettle, stir, the temperature in the reaction kettle is 40 °C, the pressure is 1.2 MPa, after stirring for 30 min, carry out reduced-pressure drying to obtain the activator;

[0036] (2) Mix the activator and the microorganism: Add Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis, Pseudomonas and the activator to a reaction kettle, the temperature in the reaction kettle is 35 °C, the pressure is 1.7 MPa, the stirring time is 1.5 h, stir evenly, and carry out reduced-pressure drying to obtain the decomposing agent of Chamaecrista rotundifolia.

[0037] Example 2

[0038] A decomposing agent of Chamaecrista rotundifolia, the microbial contents in the decomposing agent are respectively: Aspergillus niger 0.7×10 9 cfu / g, Trichothecium roseum 0.8×10 9 cfu / g, Trichoderma longibrachiatum 1.5×10 9 cfu / g, Bacillus subtilis 1.3×10 9 cfu / g, Phanerochaete chrysosporium 1.4×10 9 cfu / g, Aspergillus tubingensis 1.2×10 9 cfu / g, Pseudomonas 1.3×10 9 cfu / g; The activator comprises raw materials in the following parts by weight: 60 parts of apatite powder, 20 parts of glucose, 10 parts of minerals, and 10 parts of citric acid; the particle size of the hydroxyapatite powder is 100 μm; the mass ratio of the microorganism to the activator is 35:3.

[0039] A method for preparing a decomposing agent of Chamaecrista rotundifolia, the specific steps are as follows:

[0040] (1) Prepare the activator: Weigh citric acid, minerals and glucose by parts by weight, add water 15 times their mass, after complete dissolution, add hydroxyapatite powder, then add it to a reaction kettle, stir, the temperature in the reaction kettle is 50 °C, the pressure is 1.5 MPa, after stirring for 60 min, carry out reduced-pressure drying to obtain the activator;

[0041] (2) Mixing the activator and microorganisms: Add Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis, Pseudomonas and the activator into a reaction kettle. The temperature in the reaction kettle is 35 °C, the pressure is 1.7 MPa, and the stirring time is 1.5 h. Stir evenly and then conduct vacuum drying to obtain the decomposing microbial agent for Chamaecrista rotundifolia.

[0042] Example 3

[0043] A decomposing microbial agent for Chamaecrista rotundifolia, wherein the microbial contents in the decomposing microbial agent are as follows: Aspergillus niger 0.5 - 0.7×10 9 cfu / g, Trichothecium roseum 0.7×10 9 cfu / g, Trichoderma longibrachiatum 1.4×10 9 cfu / g, Bacillus subtilis 1.2×10 9 cfu / g, Phanerochaete chrysosporium 1.3×10 9 cfu / g, Aspergillus tubingensis 1.0×10 9 cfu / g, Pseudomonas 1.2×10 9 cfu / g; the activator contains the following raw materials by weight: 50 parts of apatite powder, 18 parts of glucose, 8 parts of minerals, 8 parts of citric acid; the particle size of the hydroxyapatite powder is 60 μm; the mass ratio of the microorganisms to the activator is 25:2.

[0044] A method for preparing a decomposing microbial agent for Chamaecrista rotundifolia, the specific steps are as follows:

[0045] (1) Prepare the activator: Weigh citric acid, minerals and glucose by weight, add 10 times the mass of water, dissolve completely, then add hydroxyapatite powder, and then add it into a reaction kettle, stir. The temperature in the reaction kettle is 45 °C, the pressure is 1.3 MPa, stir for 50 min, and then conduct vacuum drying to obtain the activator;

[0046] (2) Mixing the activator and microorganisms: Add Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis, Pseudomonas and the activator into a reaction kettle. The temperature in the reaction kettle is 28 °C, the pressure is 1.5 MPa, the stirring time is 1.2 h, stir evenly, and then conduct vacuum drying to obtain the decomposing microbial agent for Chamaecrista rotundifolia.

[0047] Experimental Example

[0048] 1. Materials and Methods

[0049] 1.1 Test materials:

[0050] Test soil: Red soil dryland orchard soil

[0051] Test fertilizer: High-carbon-based soil remediation organic fertilizer (purchased from the market)

[0052] Test green manure: Fresh Chamaecrista rotundifolia plants

[0053] Decomposition inoculant: Prepared from Example 1

[0054] Nitrogen-fixing bacteria: Purchased from the market

[0055] 1.2 Test methods

[0056] The test plot area is 20 m 2 , with 3 replicates, randomly arranged in plots, and sampled 7 times. Select a square plot with a large area, convenient drainage and irrigation, medium and uniform soil fertility, no obstacle facilities and other human impacts nearby (no fertilizer tests have been conducted on the previous crops) for dry land tests. Use 84 customized special nylon mesh bags to hold 500 - 1000 g of fresh weight of Chamaecrista rotundifolia (dry weight is about 100 - 200 g), weigh and mark, and tie a plastic label with a waterproof number on each bag. Mark the landmark signs with a benchmark connection for each bag to facilitate sampling. Place the green manure net in dry land at a depth of about 1 - 30 cm, with a length of about 30 cm and a width of 30 cm.

[0057] Treatment 1: Treat Chamaecrista rotundifolia with the decomposition inoculant prepared in Example 1.

[0058] Treatment 2: Soak Chamaecrista rotundifolia with the decomposition inoculant prepared in Example 1 and then treat it with high-carbon-based soil remediation organic fertilizer. The mass ratio of high-carbon-based soil remediation organic fertilizer to the dry weight of Chamaecrista rotundifolia can be 1 - 3:3 - 5. In this treatment, the high-carbon-based soil remediation organic fertilizer is equal in weight to the dry weight of Chamaecrista rotundifolia.

[0059] Treatment 3: Soak and treat Chamaecrista rotundifolia by adding nitrogen-fixing bacteria to the decomposition inoculant prepared in Example 1. The mass ratio of the decomposition inoculant to nitrogen-fixing bacteria can be 45 - 60:1 - 4. In this treatment, the mass ratio of the decomposition inoculant to nitrogen-fixing bacteria is 20:1.

[0060] Treatment 4: Chamaecrista rotundifolia without treatment with the decomposition inoculant.

[0061] Take fresh Chamaecrista rotundifolia samples, dry them, measure the moisture coefficient, and measure the total nitrogen, total phosphorus, total potassium, and total carbon contents. Sample the Chamaecrista rotundifolia in the above Treatments 1 - 4 on the 10th, 20th, 30th, 40th, 50th, 60th, and 70th days respectively. Mark the sampled time, treatment name, sampler and other information on the samples taken. After each sampling, wash and measure the remaining residue dry weight and the nutrient (carbon, nitrogen, phosphorus, potassium) contents in the soil at the corresponding time. Calculate the decomposition degree of Chamaecrista rotundifolia. The decomposition degree of Chamaecrista rotundifolia % = (dry weight loss of Chamaecrista rotundifolia / original dry weight of Chamaecrista rotundifolia) × 100%.

[0062] 2. Results and analysis

[0063] 2.1 Changes in the biomass of Chamaecrista rotundifolia

[0064] Figure 1 and Figure 2 respectively represent the biomass change and the cumulative decomposition rate of biomass during the decomposition process of the Chamaecrista rotundifolia plant. As can be seen from Figure 1 it, during the 1st - 10th days under different treatments, the decomposition rate of the Chamaecrista rotundifolia plant was the fastest and the biomass decline was the largest, and then gradually became flat; during the whole decomposition process, the biomass of Chamaecrista rotundifolia treated with high - carbon - based soil remediation organic fertilizer + decomposing agent decreased the most, followed by decomposing agent + nitrogen - fixing bacteria, decomposing agent, and non - application of decomposing agent treatments in sequence. As can be seen from Figure 2 it, the cumulative decomposition rate of biomass of the plants treated with high - carbon - based soil remediation organic fertilizer + decomposing agent was the highest, followed by decomposing agent + nitrogen - fixing bacteria, decomposing agent, and non - application of decomposing agent treatments in sequence; during the 1st - 10th days under different treatments, the decomposition rate was the largest, and those of high - carbon - based soil remediation organic fertilizer + decomposing agent, decomposing agent + nitrogen - fixing bacteria, decomposing agent, and non - application of decomposing agent treatments were 52.6%, 52.1%, 50.8%, and 48.4% respectively, and then gradually became flat; compared with the non - application of decomposing agent treatment, the decomposition rates of high - carbon - based soil remediation organic fertilizer + decomposing agent, decomposing agent + nitrogen - fixing bacteria, and decomposing agent treatments increased by 17.3%, 12.8%, and 8.5% respectively.

[0065] 2.2 Total carbon change of Chamaecrista rotundifolia plant

[0066] Figure 3 and Figure 4 respectively represent the plant change and the cumulative decomposition rate of total carbon during the decomposition process of the Chamaecrista rotundifolia plant. As can be seen from Figure 3 it, during the 1st - 10th days under different treatments, the decomposition rate of the Chamaecrista rotundifolia plant was the fastest and the total carbon of the plant decreased the most, and then gradually became flat; during the whole decomposition process, the total carbon of Chamaecrista rotundifolia treated with high - carbon - based soil remediation organic fertilizer + decomposing agent decreased the most, followed by decomposing agent + nitrogen - fixing bacteria, decomposing agent, and non - application of decomposing agent treatments in sequence. As can be seen from Figure 4 it, the cumulative decomposition rate of total carbon of the plants treated with high - carbon - based soil remediation organic fertilizer + decomposing agent was the highest, followed by decomposing agent + nitrogen - fixing bacteria, decomposing agent, and non - application of decomposing agent treatments in sequence; during the 1st - 10th days under different treatments, the decomposition rate was the largest, and those of high - carbon - based soil remediation organic fertilizer + decomposing agent, decomposing agent + nitrogen - fixing bacteria, decomposing agent, and non - application of decomposing agent treatments were 65.1%, 54.4%, 53.1%, and 51.7% respectively, and then gradually became flat; compared with the non - application of decomposing agent treatment, the decomposition rates of high - carbon - based soil remediation organic fertilizer + decomposing agent, decomposing agent + nitrogen - fixing bacteria, and decomposing agent treatments increased by 21.4%, 10.9%, and 9.7% respectively.

[0067] 2.3 Total nitrogen change of Chamaecrista rotundifolia plant

[0068] Figure 5 and Figure 6respectively represent the changes in total nitrogen and the cumulative decomposition rate of total nitrogen in the plant during the decomposition process of Chamaecrista rotundifolia. It can be seen from Figure 5 that during the 1st - 10th days under different treatments, the decomposition rate of Chamaecrista rotundifolia plants was the fastest, and the decline in total nitrogen of the plants was the largest, and then gradually became flat; during the entire decomposition process, the decline in total nitrogen of Chamaecrista rotundifolia plants treated with high - carbon - based soil remediation organic fertilizer + decomposition agent was the largest, followed by decomposition agent + nitrogen - fixing bacteria, decomposition agent, and non - application of decomposition agent treatments in sequence. It can be seen from Figure 6 that the cumulative decomposition rate of total nitrogen of plants treated with high - carbon - based soil remediation organic fertilizer + decomposition agent was the highest, followed by decomposition agent + nitrogen - fixing bacteria, decomposition agent, and non - application of decomposition agent treatments in sequence; during the 1st - 10th days under different treatments, the decomposition rate was the largest, and the decomposition rates of high - carbon - based soil remediation organic fertilizer + decomposition agent, decomposition agent + nitrogen - fixing bacteria, decomposition agent, and non - application of decomposition agent treatments were 65.5%, 61.5%, 60.3%, and 54.5% respectively, and then gradually became flat; compared with the non - application of decomposition agent treatment, the decomposition rates of high - carbon - based soil remediation organic fertilizer + decomposition agent, decomposition agent + nitrogen - fixing bacteria, and decomposition agent treatments increased by 9.4%, 7.5%, and 5.4% respectively.

[0069] 2.4 Changes in total phosphorus of Chamaecrista rotundifolia plants

[0070] Figure 7 and Figure 8 respectively represent the changes in total phosphorus and the cumulative decomposition rate of total phosphorus in the plant during the decomposition process of Chamaecrista rotundifolia. It can be seen from Figure 7 that during the 1st - 10th days under different treatments, the decomposition rate of Chamaecrista rotundifolia plants was the fastest, and the decline in total phosphorus of the plants was the largest, and then gradually became flat; during the entire decomposition process, the decline in total phosphorus of Chamaecrista rotundifolia plants treated with high - carbon - based soil remediation organic fertilizer + decomposition agent was the largest, followed by decomposition agent + nitrogen - fixing bacteria, decomposition agent, and non - application of decomposition agent treatments in sequence. It can be seen from Figure 8 that the cumulative decomposition rate of total phosphorus of plants treated with high - carbon - based soil remediation organic fertilizer + decomposition agent was the highest, followed by decomposition agent + nitrogen - fixing bacteria, decomposition agent, and non - application of decomposition agent treatments in sequence; during the 1st - 10th days under different treatments, the decomposition rate was the largest, and the decomposition rates of high - carbon - based soil remediation organic fertilizer + decomposition agent, decomposition agent + nitrogen - fixing bacteria, decomposition agent, and non - application of decomposition agent treatments were 49.9%, 47.3%, 45.4%, and 34.9% respectively, and then gradually became flat; compared with the non - application of decomposition agent treatment, the decomposition rates of high - carbon - based soil remediation organic fertilizer + decomposition agent, decomposition agent + nitrogen - fixing bacteria, and decomposition agent treatments increased by 12.9%, 4.7%, and 4.4% respectively.

[0071] 2.5 Changes in total potassium of Chamaecrista rotundifolia plants

[0072] Figure 9 and Figure 10 respectively represent the changes in total potassium and the cumulative decomposition rate of total potassium in the plant during the decomposition process of Chamaecrista rotundifolia. It can be seen fromFigure 9 It can be seen that during the 1st to 10th days under different treatments, the decomposition rate of the Chamaecrista rotundifolia plants was the fastest, and the decline in the total potassium of the plants was the largest, and then it gradually became flat; during the entire decomposition process, the total potassium of the Chamaecrista rotundifolia plants treated with the high-carbon-based soil remediation organic fertilizer + decomposition agent had the largest decline, followed by the decomposition agent + nitrogen-fixing bacteria, the decomposition agent, and the treatment without the decomposition agent in sequence. From Figure 10 It can be seen that the cumulative decomposition rate of the total potassium of the plants treated with the high-carbon-based soil remediation organic fertilizer + decomposition agent was the highest, followed by the decomposition agent + nitrogen-fixing bacteria, the decomposition agent, and the treatment without the decomposition agent in sequence; during the 1st to 10th days under different treatments, the decomposition rate was the largest, and the high-carbon-based soil remediation organic fertilizer + decomposition agent, the decomposition agent + nitrogen-fixing bacteria, the decomposition agent, and the treatment without the decomposition agent were 74.1%, 71.3%, 71.0%, and 61.4% respectively, and then it gradually became flat; compared with the treatment without the decomposition agent, the decomposition rates of the high-carbon-based soil remediation organic fertilizer + decomposition agent, the decomposition agent + nitrogen-fixing bacteria, and the decomposition agent treatments increased by 0.9%, 0.8%, and 0.8% respectively. Different treatments had little effect on the release of potassium in the plants.

[0073] In summary, the decomposition agent prepared by the technical solution of the present invention can improve the decomposition rates of the biomass, total carbon, total nitrogen, and total phosphorus of the Chamaecrista rotundifolia; although the decomposition agent has little effect on the total cumulative decomposition rate of the total potassium of the Chamaecrista rotundifolia, during the 1st to 10th days after the Chamaecrista rotundifolia was treated with the decomposition agent, the decomposition rate of the total potassium of the plants in the decomposition agent treatment group was greater than that in the group without the decomposition agent treatment; based on the comprehensive evaluation of the cumulative decomposition rates of the biomass, total carbon, total nitrogen, total phosphorus, and total potassium, the decomposition agent disclosed in the present invention has a good effect on decomposing the Chamaecrista rotundifolia. Preferably, adding the high-carbon-based remediation organic fertilizer or nitrogen-fixing bacteria to the decomposition of the Chamaecrista rotundifolia by the decomposition agent has a better effect than using the decomposition agent alone.

Claims

1. A roundleaf tickclover decomposition microbial agent, characterized in that, The decomposition bacterial agent contains Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, white rot fungus, Aspergillus tubingensis, Pseudomonas and an activator; The contents of Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, Phanerochaete chrysosporium, Aspergillus tubingensis and Pseudomonas in the putrefactive bacteria agent are respectively: Aspergillus niger 0.5 - 0.7×10 9 cfu / g, Trichothecium roseum 0.6 - 0.8×10 9 cfu / g, Trichoderma longibrachiatum 1.2 - 1.5×10 9 cfu / g, Bacillus subtilis 1.0 - 1.3×10 9 cfu / g, Phanerochaete chrysosporium 1.2 - 1.4×10 9 cfu / g, Aspergillus tubingensis 0.9 - 1.2×10 9 cfu / g and Pseudomonas 1.1 - 1.3×10 9 cfu / g; the activator comprises the following raw materials in parts by weight: 40 - 60 parts of apatite powder, 15 - 20 parts of glucose, 5 - 10 parts of minerals and 5 - 10 parts of citric acid; The preservation number of Aspergillus niger is GDMCC NO.3.250; the preservation number of Trichothecium roseum is GDMCC NO.3.500; the preservation number of Trichoderma longibrachiatum is GDMCC NO.3.140; the preservation number of Bacillus subtilis is GDMCC NO.1.1665; the preservation number of white rot fungus is GDMCC NO.3.383; the preservation number of Aspergillus tubingensis is GDMCC NO.3.577; the preservation number of Pseudomonas is GDMCC NO.1.462; The mass ratio of Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, white rot fungus, Aspergillus tubingensis and Pseudomonas to the activator is 20-35:1-3.

2. The aeschynomene rot decomposing microbial agent according to claim 1, wherein The apatite powder is hydroxyapatite powder with a particle size of 50-100 μm.

3. A method for preparing the Corchorus rotundus L. decomposing microbial agent according to any one of claims 1-2, characterized in that, The decomposition bacterial agent is prepared by the following steps: (1) Preparation of the activator: Weigh citric acid, minerals and glucose by weight, add water 5-15 times their mass, dissolve completely, add apatite powder, then add it to a reaction kettle, stir, the temperature in the reaction kettle is 40-50 °C, the pressure is 1.2-1.5 MPa, stir for 30-60 min, and then carry out vacuum drying to obtain the activator; (2) Mixing the activator and microorganisms: Add Aspergillus niger, Trichothecium roseum, Trichoderma longibrachiatum, Bacillus subtilis, white rot fungus, Aspergillus tubingensis, Pseudomonas and the activator to a reaction kettle, the temperature in the reaction kettle is 20-35 °C, the pressure is 1.3-1.7 MPa, the stirring time is 1-1.5 h, stir evenly, and carry out vacuum drying to obtain the decomposition bacterial agent for Chamaecrista rotundifolia.

4. Application of a decomposition bacterial agent according to any one of claims 1-2 in decomposing Chamaecrista rotundifolia.

5. The application according to claim 4, wherein The specific application method is as follows: (1) Pretreatment: Wash the sundries adhering to the surface of Chamaecrista rotundifolia and set aside after drying; (2) Soaking: Soak Chamaecrista rotundifolia in the bacterial water containing the decomposition bacterial agent for 5-8 min; (3) Decomposition: Turn and press the soaked Chamaecrista rotundifolia into the soil.

6. The application of the putrefying microbial agent according to claim 5 in putrefying Chamaecrista rotundifolia, characterized in that, Add nitrogen-fixing bacteria to the decomposition bacterial agent in step (2), and the mass ratio of the decomposition bacterial agent to the nitrogen-fixing bacteria is 45-60:1-4.

7. Use of the putrefying microbial agent according to claim 5 in putrefying Chamaecrista rotundifolia, characterized in that, Add high-carbon-based soil remediation organic fertilizer in step (3).

8. The application of the putrefying microbial agent according to claim 7 in putrefying Chamaecrista rotundifolia, characterized in that, The mass ratio of the high-carbon-based soil remediation organic fertilizer to the dry weight of Chamaecrista rotundifolia is 1-3:3-5.

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