A B10 cell detection device

Abstract

The present invention discloses a B10 cell detection device, including a main control module, the main control module includes two contacts, the two contacts are electrically connected to a power supply module and a sampling module, the main control module is composed of an embedded microprocessor and a data storage card, the power supply module between the power supply module and the main control module is an input end, used for the power supply work of the main control module and its electrical connection structure, the sampling module between the sampling module and the main control module is an output end, used for extracting the periodontal inflammation tissue fluid required for detection. The present invention can simultaneously eliminate the impurities and foreign matter contained in the periodontal inflammation tissue fluid and the vacuoles on the surface of the tissue fluid by setting a filtering module and a defoaming module, prevent the two from interfering with the B10 cell detection result, make the detection result more accurate, and help dental practitioners better treat patients' periodontal inflammation.

Description

Technical Field

[0001] The present invention relates to the technical field of cell detection, and in particular to a B10 cell detection device. Background Art

[0002] Regulatory B cells are a type of B cell subset that has a negative immune regulatory effect. Among B cells, the regulatory B cells that secrete IL-10 and can exert immune regulatory effects through IL-10 are called B10 cells. B10 cells are involved in regulating the immune response of periodontitis, and can inhibit the expression of inflammatory factors such as TNF-α, IL-1β, RANKL that promote osteoclast activation and inhibit the absorption of alveolar bone. They play a vital role in the immune response of periodontitis. Therefore, the detection of B10 cells is undoubtedly very critical.

[0003] In the prior art, the detection method for B10 cells is to extract periodontal inflammation tissue fluid, and then directly perform ultrasonic detection on the extracted tissue fluid, and use the reflected ultrasonic information to obtain B10 cell data in the tissue fluid. However, when the tissue fluid is extracted, it will contain impurities and foreign matter. Direct placement for detection will affect the accuracy of the detection. In addition, during the transfer of the tissue fluid, more vacuoles will be generated on the liquid surface. These vacuoles also greatly interfere with the detection results. Therefore, it is necessary to propose a B10 cell detection device to solve the above problems. Summary of the invention

[0004] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a B10 cell detection device.

[0005] In order to achieve the above object, the present invention adopts the following technical solutions:

[0006] A B10 cell detection device comprises a main control module, the main control module comprises two contacts, the two contacts are electrically connected to a power supply module and a sampling module respectively, the main control module is composed of an embedded microprocessor and a data storage card, the power supply module is an input end between the power supply module and the main control module, and is used for powering the main control module and its electrical connection structure, the sampling module is an output end between the sampling module and the main control module, and is used for extracting periodontal inflammation tissue fluid required for detection, the sampling module is connected to a filtering module, and the filtering module is used for filtering impurities contained in the extracted periodontal inflammation tissue fluid The filtering module is electrically connected to a defoaming module, the defoaming module is used to remove floating bubbles on the surface of the extracted periodontitis tissue fluid, the defoaming module is electrically connected to a detection module, the detection module is used to detect cell data in the periodontitis tissue fluid, the detection module is electrically connected to a collection module, the collection module is used to collect the cell data detected by the detection module, the collection module is electrically connected to an analysis module, the analysis module is used to analyze the B10 cell data contained in the cell data, the analysis module is electrically connected to a recording module, the recording module is used to record the B10 cell data analyzed by the analysis module.

[0007] Preferably, the filtration module includes a liquid collector and a filter bottle, a filter net is fixedly connected between the inner walls of the lower end of the filter bottle, the filter bottle is connected to the liquid collector through a liquid inlet pipe, and the two ends of the liquid inlet pipe are respectively located at the lower ends of the liquid collector and the filter bottle, and the upper end of the filter bottle is connected to a first liquid outlet pipe.

[0008] Preferably, the debubble module includes a debubble bottle and a detector, the lower end of the debubble bottle is connected to the first liquid outlet pipe, a liquid pump is arranged between the debubble bottle and the detector, the liquid inlet end of the liquid pump is connected to the lower end of the debubble bottle through the second liquid outlet pipe, the liquid outlet end of the liquid pump is connected to the detector through the third liquid outlet pipe, the upper end of the debubble bottle is fixedly connected to the device chamber, and a debubble mechanism for puncturing floating bubbles on the liquid surface is installed in the debubble bottle.

[0009] Preferably, the debubble mechanism comprises a sliding plate slidably connected between the inner walls of the upper end of the debubble bottle, the lower end of the sliding plate is provided with spikes, and a first pushing mechanism for pushing the sliding plate to reciprocate in a vertical direction is installed in the device chamber.

[0010] Preferably, the first pushing mechanism includes a sliding plug and a connecting rod slidably connected between the inner walls of the device chamber, one end of the connecting rod is fixedly connected to the lower end of the sliding plug, and the other end of the connecting rod is fixedly connected to the upper end of the sliding plate, a motor is fixedly installed on the side wall of the device chamber, and a second pushing mechanism for pushing the sliding plug to reciprocate in the vertical direction is installed in the device chamber.

[0011] Preferably, the second pushing mechanism includes a rotating wheel and a rotating rod, the rotating wheel is rotatably connected to the inner wall of the device chamber and is coaxially fixedly connected to the motor output shaft, one end of the rotating rod is rotatably connected to the non-axial side wall of the rotating wheel, and the other end of the rotating rod is rotatably connected to the upper end of the sliding plug.

[0012] Preferably, the detection module includes an ultrasonic transmitting unit, a carrying unit and an ultrasonic collecting unit, the carrying unit is used to carry periodontal inflammatory tissue fluid, the ultrasonic transmitting unit is used to generate ultrasonic waves to the periodontal inflammatory tissue fluid on the carrying unit, and the ultrasonic collecting unit is used to collect ultrasonic waves reflected by the periodontal inflammatory tissue fluid.

[0013] The present invention has the following beneficial effects:

[0014] 1. By setting up a filtration module, the tissue fluid can be transferred through the filter bottle, so that the tissue fluid passes through the filter net in the filter bottle. During the penetration process, the impurities and foreign matter contained in the tissue fluid will be filtered by the filter net, thereby ensuring that the tissue fluid put into the subsequent test will not have foreign matter and impurities that interfere with the B10 cell test results;

[0015] 2. By setting up a debubble module, the motor drives the rotating wheel to rotate, and the rotating wheel drives the sliding plug to move back and forth up and down through the rotating rod. The sliding plug pushes the sliding plate to move back and forth up and down through the connecting rod, so that the sharp thorns can puncture the vacuoles on the surface of the tissue fluid, thereby eliminating the influence of the vacuoles on the B10 cell detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a system block diagram of a B10 cell detection device proposed by the present invention;

[0017] Figure 2 A system block diagram of the detection module proposed by the present invention;

[0018] Figure 3 This is a schematic diagram of the structure of the filtering module proposed by the present invention;

[0019] Figure 4 This is a schematic diagram of the structure of the defoaming module proposed in the present invention;

[0020] Figure 5 for Figure 4 A schematic diagram of the enlarged structure of .

[0021] In the figure: 1, liquid collector; 2, filter bottle; 3, liquid inlet pipe; 4, filter screen; 5, first liquid outlet pipe; 6, defoaming bottle; 7, detector; 8, liquid pump; 9, second liquid outlet pipe; 10, third liquid outlet pipe; 11, sliding plate; 12, spikes; 13, device chamber; 14, sliding plug; 15, connecting rod; 16, motor; 17, rotating wheel; 18, rotating rod. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0023] In the description of the present invention, it is necessary to understand that the terms "upper", "lower", "front", "back", "left", "right", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship are based on the orientation or position relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0024] Reference Figure 1-5 A B10 cell detection device includes a main control module, which includes two contacts, and the two contacts are electrically connected to a power supply module and a sampling module respectively. The main control module is composed of an embedded microprocessor and a data storage card. The power supply module is an input end between the power supply module and the main control module, which is used for powering the main control module and its electrical connection structure. The sampling module is an output end between the sampling module and the main control module, which is used for extracting the periodontal inflammation tissue fluid required for detection. The sampling module is connected to a filtering module, which is used to filter impurities contained in the extracted periodontal inflammation tissue fluid. The filtering module includes a liquid collector 1 and a filtering bottle 2. A filter screen 4 is fixedly connected between the inner walls of the lower end of the filtering bottle 2. The filtering bottle 2 is connected to the liquid collector 1 through a liquid inlet pipe 3, and the two ends of the liquid inlet pipe 3 are respectively located at the lower ends of the liquid collector 1 and the filtering bottle 2. The upper end of the filtering bottle 2 is connected to a first liquid outlet pipe 5.

[0025] The filtering module is electrically connected to a debubble module, which is used to remove floating bubbles on the liquid surface of the extracted periodontitis tissue fluid. The debubble module includes a debubble bottle 6 and a detector 7. The lower end of the debubble bottle 6 is connected to the first liquid outlet pipe 5. A liquid pump 8 is arranged between the debubble bottle 6 and the detector 7. The liquid inlet end of the liquid pump 8 is connected to the lower end of the debubble bottle 6 through the second liquid outlet pipe 9, and the liquid outlet end of the liquid pump 8 is connected to the detector 7 through the third liquid outlet pipe 10. The upper end of the debubble bottle 6 is fixedly connected to a device chamber 13, and a debubble mechanism for puncturing floating bubbles on the liquid surface is installed in the debubble bottle 6.

[0026] The defoaming mechanism includes a sliding plate 11 slidably connected between the inner walls of the upper end of the defoaming bottle 6, and a spike 12 is provided at the lower end of the sliding plate 11. A first pushing mechanism for pushing the sliding plate 11 to reciprocate in the vertical direction is installed in the device chamber 13.

[0027] The first pushing mechanism includes a sliding plug 14 and a connecting rod 15 which are slidably connected between the inner walls of the device chamber 13. One end of the connecting rod 15 is fixedly connected to the lower end of the sliding plug 14, and the other end of the connecting rod 15 is fixedly connected to the upper end of the sliding plate 11. A motor 16 is fixedly installed on the side wall of the device chamber 13. A second pushing mechanism for pushing the sliding plug 14 to reciprocate in the vertical direction is installed in the device chamber 13.

[0028] The second pushing mechanism includes a rotating wheel 17 and a rotating rod 18. The rotating wheel 17 is rotatably connected to the inner wall of the device chamber 13 and is coaxially fixedly connected to the output shaft of the motor 16. One end of the rotating rod 18 is rotatably connected to the non-axial part of the side wall of the rotating wheel 17, and the other end of the rotating rod 18 is rotatably connected to the upper end of the sliding plug 14.

[0029] The defoaming module is electrically connected to a detection module, which is used to detect cell data in periodontitis tissue fluid. The detection module includes an ultrasonic transmitting unit, a carrying unit and an ultrasonic collecting unit. The carrying unit is used to carry the periodontitis tissue fluid, the ultrasonic transmitting unit is used to generate ultrasonic waves to the periodontitis tissue fluid on the carrying unit, and the ultrasonic collecting unit is used to collect ultrasonic waves reflected by the periodontitis tissue fluid.

[0030] The detection module is electrically connected to a collection module, which is used to collect the cell data detected by the detection module. The collection module is used to collect the cell data detected by the detection module. The collection module is electrically connected to an analysis module, which is used to analyze the B10 cell data contained in the cell data. The analysis module is electrically connected to a recording module, which is used to record the B10 cell data analyzed by the analysis module.

[0031] In the present invention, when it is necessary to detect B10 cells in periodontal inflammation tissue fluid, the sampling module can be used to extract the tissue fluid and place the tissue fluid into the liquid collector 1. Then, under the action of the liquid pump 8, when the tissue fluid passes through the filter bottle 2, the filter net 4 in the filter bottle 2 filters the tissue fluid to remove impurities and foreign matter, thereby avoiding the influence of impurities and foreign matter on the detection results.

[0032] Then the tissue fluid passes through the debubble bottle 6, the motor 16 is started, the motor 16 drives the rotating wheel 17 to rotate, the rotating wheel 17 drives the sliding plug 14 to move back and forth up and down through the rotating rod 18, and the sliding plug 14 pushes the sliding plate 11 to move back and forth up and down through the connecting rod 15, so that the spike 12 can puncture the vacuole on the surface of the tissue fluid, thereby eliminating the influence of the vacuole on the B10 cell detection result.

[0033] The tissue fluid can then enter the detection module for testing, using ultrasound to obtain B10 cell data information and record it to help dental practitioners better treat periodontal inflammation.

[0034] The above description is only a preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can make equivalent replacements or changes according to the technical scheme and inventive concept of the present invention within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A B10 cell detection device, comprising a main control module, characterized in that: The main control module includes two contacts, and the two contacts are electrically connected to a power supply module and a sampling module respectively. The main control module is composed of an embedded microprocessor and a data storage card. The power supply module between the power supply module and the main control module is an input end, which is used for powering the main control module and its electrical connection structure. The sampling module between the sampling module and the main control module is an output end, which is used for extracting the periodontal inflammation tissue fluid required for detection. The sampling module is connected to a filtering module, which is used to filter impurities contained in the extracted periodontal inflammation tissue fluid. The filtering module is electrically connected to a defoaming module, which is used to remove bubbles on the surface of the extracted periodontal inflammation tissue fluid. The defoaming module is electrically connected to a detection module, the detection module is used to detect cell data in periodontitis tissue fluid, the detection module is electrically connected to a collection module, the collection module is used to collect cell data detected by the detection module, the collection module is electrically connected to an analysis module, the analysis module is used to analyze B10 cell data contained in the cell data, the analysis module is electrically connected to a recording module, the recording module is used to record the B10 cell data analyzed by the analysis module; the filtering module includes a liquid collector and a filtering bottle, a filtering net is fixedly connected between the inner walls of the lower end of the filtering bottle, the filtering bottle is connected to the liquid collector through a liquid inlet pipe, and the two ends of the liquid inlet pipe are respectively located at the collecting The lower end of the liquid container and the filter bottle is connected with a first liquid outlet pipe at the upper end of the filter bottle; the debubble module includes a debubble bottle and a detector, the lower end of the debubble bottle is connected with the first liquid outlet pipe, a liquid pump is arranged between the debubble bottle and the detector, the liquid inlet end of the liquid pump is connected with the lower end of the debubble bottle through the second liquid outlet pipe, and the liquid outlet end of the liquid pump is connected with the detector through the third liquid outlet pipe; the upper end of the debubble bottle is fixedly connected with a device chamber, and a debubble mechanism for puncturing floating bubbles on the liquid surface is installed in the debubble bottle; the debubble mechanism includes a sliding plate slidably connected between the inner walls of the upper end of the debubble bottle, and the lower end of the sliding plate is provided with spikes, and a device for pushing the sliding plate to move in a vertical direction is installed in the device chamber. A first pushing mechanism for reciprocating motion; the first pushing mechanism includes a sliding plug and a connecting rod slidably connected between the inner walls of the device chamber, one end of the connecting rod is fixedly connected to the lower end of the sliding plug, and the other end of the connecting rod is fixedly connected to the upper end of the sliding plate, a motor is fixedly installed on the side wall of the device chamber, and a second pushing mechanism for pushing the sliding plug to reciprocate in the vertical direction is installed in the device chamber; the second pushing mechanism includes a rotating wheel and a rotating rod, the rotating wheel is rotatably connected to the inner wall of the device chamber and is coaxially fixedly connected to the output shaft of the motor, one end of the rotating rod is rotatably connected to the non-axial part of the side wall of the rotating wheel, and the other end of the rotating rod is rotatably connected to the upper end of the sliding plug;The detection module includes an ultrasonic transmitting unit, a carrying unit and an ultrasonic collecting unit, wherein the carrying unit is used to carry periodontal inflammation tissue fluid, the ultrasonic transmitting unit is used to generate ultrasonic waves to the periodontal inflammation tissue fluid on the carrying unit, and the ultrasonic collecting unit is used to collect ultrasonic waves reflected by the periodontal inflammation tissue fluid. ;

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