Microbial drug sensitivity detection image acquisition system

Abstract

The present invention discloses a microbial drug sensitivity detection image acquisition system, including a base, on which a light source adjustment unit, an image acquisition unit and an image adjustment unit are arranged. The present invention has the advantages of realizing rapid detection of drug sensitivity reactions, low cost, clear images, rapid light adjustment, simple operation, and low requirements on light sources. At the same time, the growth and reproduction of bacterial strains can be observed in real time, which can be applied to drug sensitivity detection to improve detection efficiency; it takes into account the characteristics of large field of view and high resolution, with a resolution of 0.5um, and a field of view that is consistent with the size of a CMOS chip, up to 1 inch, much higher than a microscope objective; it realizes precise positioning, which is conducive to the acquisition of bacterial shadow images.

Description

Technical Field

[0001] The present invention relates to the field of microbial detection, and in particular to an image acquisition system for microbial drug sensitivity detection. Background Art

[0002] There are two types of existing drug sensitivity tests. One is the manual operation method, which takes a long time, is inefficient, requires high professional quality of personnel, has strong subjective consciousness, and is prone to errors. The other is to rely on automated instruments, such as BD, Beckman and other instruments, which detect growth or color changes through optical modules. However, there is a big flaw, that is, it takes a long time, generally 8-16 hours, and is expensive, which is a heavy burden for small and medium-sized hospitals. Summary of the invention

[0003] The present invention aims to provide a microbial drug sensitivity detection image acquisition system, aiming to provide a fast and low-cost image acquisition device for drug sensitivity detection, so as to realize rapid detection of drug sensitivity reactions.

[0004] To achieve the above purpose, the present invention can adopt the following technical solutions:

[0005] The microbial drug sensitivity detection image acquisition system of the present invention comprises a base station, on which a light source adjustment unit, an image acquisition unit and an image adjustment unit are arranged;

[0006] The light source adjustment unit comprises

[0007] A light source sleeve, used for emitting parallel light vertically downward;

[0008] An angle adjustment device, used to adjust the incident angle of the optical axis of the light source sleeve so that the optical axis is centered;

[0009] A first slide, used for fixing the light source sleeve to move up and down along the first slide; the first slide is fixedly connected to the angle adjustment device through a first support, and the angle adjustment device is fixedly connected to the base through a support;

[0010] The image acquisition unit comprises an acquisition platform and an image sensor arranged on the platform, wherein the image sensor is used to acquire sample images;

[0011] The image adjustment unit is used to adjust the position of the image acquisition unit to obtain the best shooting position; the image adjustment unit includes

[0012] The second support member is used to fix the collection platform; the second support member is provided with a second slide, and the second slide is slidably matched with the second slide, thereby forming an up and down moving mechanism; the second slide is fixedly connected to the third slide through a support plate, and the third slide is arranged on the third slide slidably matched with the third slide, thereby forming a left and right horizontal moving mechanism; the third slide is fixed on the fourth slide, and the fourth slide is arranged on the fourth slide slidably matched with the fourth slide, thereby forming a front and back horizontal moving mechanism, and the fourth slide is fixed to the base.

[0013] Furthermore, a dial is provided on the first support member for indicating the incident angle of the optical axis of the light source sleeve.

[0014] Preferably, the light source sleeve includes a light source, an optical micropore, an optical lens and an adjustment knob; the light source uses a quasi-monochromatic LED light source; the optical micropore is used to control the size of the light emitting area of ​​the light source; the optical micropore is placed at the focus of the optical lens, so that the light emitted by the micropore becomes parallel light, and the light divergence angle is ≤ plus or minus 5 degrees.

[0015] Furthermore, an adjusting bolt is respectively arranged on the four corners of the bottom surface of the base, for supporting and adjusting the horizontal state of the base; a spare storage table is arranged on the base, for placing a spare light source sleeve, so as to facilitate the rapid replacement of the light source sleeve.

[0016] The advantages of the present invention are that it can quickly detect drug sensitivity reactions. At the same time, the system has low cost, clear images, can quickly adjust the light, is easy to operate, and has low requirements on the light source. Specifically:

[0017] 1. The present invention can observe the growth and reproduction of bacterial strains in real time, and is applied to drug sensitivity detection to improve detection efficiency;

[0018] 2. The present invention takes into account the characteristics of large field of view and high resolution, with a resolution of 0.5um. The field of view is consistent with the size of CMOS chip, up to 1 inch, which is much higher than the microscope objective lens;

[0019] 3. The present invention removes the CMOS surface protection glass and the Bayer color lens array, reducing the distance between the sample and the CMOS to 300um;

[0020] 4. The present invention pastes a high-transmittance film seat on the surface of the CMOS chip as a disposable consumable, and the film is TC-treated to reduce the risk of CMOS damage, greatly reducing the cost of the entire system;

[0021] 5. The present invention adds micro-holes and shaping lenses to control the light-emitting angle of the LED light source, thereby reducing the interference of the inclined light on the shadow imaging;

[0022] 6. This system has a micron-level adjustment control device that can accurately control the spacing between each device to achieve precise positioning, which is conducive to the acquisition of bacterial shadow images. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 It is a schematic diagram of the structure of the present invention (the spare storage table is hidden).

[0024] Figure 2 It is an axonometric structural schematic diagram of the present invention.

[0025] Figure 3 It is an operation flow chart of the present invention. DETAILED DESCRIPTION

[0026] like Figure 1-2 As shown, a microbial drug sensitivity detection image acquisition system includes a base 4, a light source adjustment unit, an image acquisition unit and an image adjustment unit arranged on the base 4.

[0027] The light source adjustment unit includes an angle adjustment device 1.1, which is used to adjust the incident angle of the optical axis through the optical axis centering knob 1.2 on the angle adjustment device 1.1, so that the optical axis of the light source is centered; a tripod support 1.3, which is fixed to the base 4 through bolts, and the other end is fixedly connected to the angle adjustment device 1.1, and is used to support the entire light source adjustment unit; a first slide 1.4 cooperates with a first slide seat 1.5 to form an adjustment structure that can move up and down, and the first slide seat 1.5 is connected and fixed to the dial of the angle adjustment device 1.1 through a first support member 1.6, and the dial is used to indicate the incident angle of the optical axis of the light source sleeve 1.8, and the light source sleeve 1.8 is moved up and down by adjusting the adjustment knob 1.7 on the first slide 1.4; the light source sleeve 1.8 is fixed to the fixing member 1.10 through a detachable bolt 1.9, and is used to realize the rapid replacement of the light source sleeve 1.8; the other end of the fixing member 1.10 is connected and fixed to the first slide 1.4.

[0028] The light source sleeve 1.8 includes a light source, an optical micropore, an optical lens and an adjustment knob 1.11; the light source uses a quasi-monochromatic LED light source, and this embodiment uses the Silicon Valley Light Engine LZ1-00R102 model; the optical micropore is used to control the size of the light emitting area of ​​the light source, and this embodiment uses an optical micropore with a light transmission diameter of 300um manufactured by Shiqiang Technology, so that the optical micropore can be regarded as a point light source; the optical lens uses an EDMUND optics LENS PCX 12MM DIA x 12MM FL VIS-NIR plano-convex lens, and the optical micropore is placed at the focus of the lens, so that the light emitted by the micropore becomes approximately parallel light, and the light divergence angle is ≤ plus or minus 5 degrees.

[0029] The image acquisition unit includes an image sensor 2.1, which is fixed on the acquisition platform 2.2 and is used to acquire sample images; the acquisition platform 2.2 is fixed to the second support member 2.3 through a card slot connection to facilitate the loading and unloading of the acquisition platform 2.2; the second support member 2.3 is fixed on the second slide 3.1 of the image adjustment unit and is used to adjust the position of the image acquisition unit to obtain the best shooting position.

[0030] The image sensor 2.1 adopts the commonly used complementary metal oxide semiconductor (CMOS) as the platform photosensitive module, and removes the CMOS surface protection glass and the Bayer color lens array, thereby reducing the distance between the sample and the CMOS to 300um; this embodiment adopts a 0.5um pixel size CMOS chip, Vipai's vertically charge-transferring Pixel Sensors (VPS), with a pixel size of 0.5um, and removes the CMOS chip surface protection glass, making the collected image clearer and with a higher resolution. The resolution of the entire image acquisition system can reach 0.5um, which meets the general bacteria detection requirements.

[0031] The image adjustment unit includes a second slide 3.1 connected and fixed to the second support member 2.3, the second slide 3.1 and the second slide seat 3.2 form an up-and-down movement mechanism by sliding cooperation, and the image sensor 2.1 is moved up and down by adjusting the adjustment knob 3.3 on the second slide seat 3.2; the third slide 3.4 is fixedly connected to the second slide seat 3.2 through an L-shaped support plate 3.5, the third slide 3.4 and the third slide seat 3.6 form a left-right horizontal movement mechanism by sliding cooperation, and the image sensor 2.1 is moved left and right by adjusting the adjustment knob 3.7 on the third slide seat 3.6. The fourth slide 3.8 is fixedly connected to the third slide 3.6, and the fourth slide 3.8 and the fourth slide 3.9 form a front-to-back horizontal movement mechanism through sliding cooperation. The front-to-back horizontal movement of the image sensor 2.1 is achieved by adjusting the adjustment knob 3.10 on the fourth slide 3.9; the fixing plate 3.11 is fixedly connected to the fourth slide 3.9 and the base 4 respectively, and the entire image adjustment unit is fixed on the base 4; the bottom surfaces of the second slide 3.2, the third slide 3.6 and the fourth slide 3.9 are provided with adjustment bolts for positioning after the adjustment is completed to prevent the slides from sliding.

[0032] An adjusting bolt 4.1 is respectively arranged on the four corners of the bottom surface of the base 4 for supporting and adjusting the horizontal state of the base 4. A spare storage table 4.2 is arranged on the base 4 for placing a spare light source sleeve 4.3 to facilitate the rapid replacement of the light source sleeve 1.8.

[0033] The specific usage of this microbial drug sensitivity detection image acquisition system is as follows:

[0034] Step 1: Adjust the adjustable anchor bolts 4.1 to make the base 4 level.

[0035] Step 2: Turn on the light source and adjust the distance between the lens of the image adjustment unit and the CMOS so that the entire image acquisition area is illuminated.

[0036] Step 3: Adjust the optical axis centering knob 1.2 to center the optical axis.

[0037] Step 4: Use the adjustment knob 1.11 on the top of the light source sleeve 1.8 to adjust the distance between the light source and the microholes to ensure uniform illumination in the image acquisition area.

[0038] Step 5, stick a disposable film on the CMOS surface with the protective glass removed. The film has been treated with TC hydrophilicity to facilitate the growth of adherent bacteria and long-term observation. Then add the sample and let it stand for 2 minutes.

[0039] Step 6: Adjust the CMOS exposure time so that the overall brightness of the image meets the normal needs of the human eye.

[0040] Step 7, take a photo, save the image, and complete the image acquisition.

[0041] Step 8, tear off the film and repeat steps 5-7.

[0042] When the microbial drug sensitivity detection image acquisition system acquires images, it only needs to be adjusted once to realize multiple detections, thereby achieving the purpose of quickly acquiring drug sensitivity reaction images and realizing rapid detection of drug sensitivity reactions; if the light source needs to be replaced, it is only necessary to unscrew the detachable bolt 1.9 and replace it with the spare light source sleeve 4.3, which is simple and convenient to operate; the system has a simple structure and a low cost, which reduces the cost of drug sensitivity detection and realizes low-cost detection of drug sensitivity reactions.

Claims

1. A microbial drug sensitivity detection image acquisition system, comprising a base station, characterized in that: The base is provided with a light source adjustment unit, an image acquisition unit and an image position adjustment unit; The light source adjustment unit comprises A light source sleeve, used for emitting parallel light vertically downward; It includes a light source, an optical microhole, an optical lens and an adjustment knob; the light source uses a quasi-monochromatic LED light source; The optical micropores are used to control the light emitting area of ​​the light source; The optical microhole is placed at the focus of the optical lens, so that the light emitted from the microhole becomes parallel light, and the light divergence angle is ≤ plus or minus 5 degrees; An angle adjustment device, used to adjust the incident angle of the optical axis of the light source sleeve so that the optical axis is centered; A first slide, used for fixing the light source sleeve to move up and down along the first slide; the first slide is fixedly connected to the angle adjustment device through a first support, and the angle adjustment device is fixedly connected to the base through a support; The image acquisition unit includes an acquisition platform and an image sensor arranged on the platform, and the image sensor is used to acquire sample images; the image sensor is a CMOS image sensor, and the CMOS surface protection glass and the Bayer color lens array are removed, so that the distance between the sample and the CMOS is reduced to 300um; a high-transmittance film seat is pasted on the surface of the CMOS chip as a disposable consumable, and the film is TC-treated; The image adjustment unit is used to adjust the position of the image acquisition unit to obtain the best shooting position; the image adjustment unit includes A second support member is used to fix the collection platform; a second slide is arranged on the second support member, and the second slide is connected to the second slide by a second slide that is slidably matched with the second slide, thereby forming an up-and-down moving mechanism; the second slide is fixedly connected to the third slide by a support plate, and the third slide is arranged on the third slide that is slidably matched with the second slide, thereby forming a left-right horizontal moving mechanism; the third slide is fixed on the fourth slide, and the fourth slide is arranged on the fourth slide that is slidably matched with the second slide, thereby forming a front-and-back horizontal moving mechanism, and the fourth slide is fixed to the base; The acquisition method of the microbial drug sensitivity detection image acquisition system is: Step 1, adjust the adjustable anchor bolts to make the base level; Step 2, turn on the light source and adjust the distance between the lens of the image adjustment unit and the CMOS so that the entire image acquisition area is illuminated; Step 3, adjust the optical axis centering knob to center the optical axis; Step 4: Use the adjustment knob on the top of the light source sleeve to adjust the distance between the light source and the microhole to ensure uniform illumination in the image acquisition area; Step 5, stick a disposable film on the CMOS surface with the protective glass removed. The film is treated with TC hydrophilicity to facilitate the growth of adherent bacteria and long-term observation. Then drop the sample and let it stand. Step 6: Adjust the CMOS exposure time so that the overall brightness of the image meets the normal needs of the human eye; Step 7, take a photo, save the image, and complete the image acquisition; Step 8, tear off the disposable consumable film and repeat steps 5-7.

2. The microbial drug sensitivity detection image acquisition system according to claim 1, wherein: The first supporting member is provided with a dial for indicating the incident angle of the optical axis of the light source sleeve.

3. The microbial drug sensitivity detection image acquisition system according to claim 1 or 2, wherein: An adjusting bolt is respectively arranged on the four corners of the bottom surface of the base, which is used to support and adjust the horizontal state of the base; a spare storage table is arranged on the base, which is used to place a spare light source sleeve, so as to facilitate the rapid replacement of the light source sleeve.

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