Microscopic sample preparation device and cell phenotype control device

Abstract

The present invention provides a microscopic sample preparation device and a cell phenotype control device, comprising: a molding plate, having a plurality of molding holes penetrating two opposite surfaces of the molding plate, for molding a plurality of solid culture media; a sample pool plate, having a plurality of sample holes penetrating two opposite surfaces of the sample pool plate, for accommodating a plurality of solid culture media; a cover glass, for being placed on one surface of the sample pool plate; and a pressing plate, provided with a plurality of pressing columns, for pressing a plurality of solid culture media in a plurality of molding holes into a plurality of sample holes and onto the cover glass. The present invention can realize the preparation of a plurality of solid culture media at one time, complete the pressing operation of a plurality of samples at one time, save time, effectively improve the experimental efficiency, and is simple, convenient, and quick to operate, and is not prone to operating errors or crushing the cover glass.

Description

Technical Field

[0001] The invention relates to the technical field of microscopic experiments, in particular to a microscopic sample preparation device and a cell phenotype control device. Background Art

[0002] In the process of biological or medical experiments, in order to better observe single bacteria or cells under a microscope, the following sample preparation process is often used: drop the bacterial / cell suspension on the cut solid culture medium (such as agar block); after the agar surface is slightly dried, press the side of the agar block with the suspension on the glass cover slip; then place it on an inverted microscope to observe the bacteria or cells. The process described above can be called the tableting process.

[0003] The above experimental process requires manual operations such as cutting agar blocks, dropping bacteria / cell suspension on agar blocks, and pressing the agar blocks with suspension on glass coverslips. Experimenters must operate carefully to obtain samples with better preparation effects. For example, the glass coverslips may be crushed. In addition, when pressing a large number of experimental samples, they need to operate one by one. The whole experimental process is time-consuming and inefficient, consuming a lot of time and energy of experimenters, and is prone to operational errors, which seriously affects the progress of scientific research or medical diagnosis. Summary of the invention

[0004] In order to solve the problems pointed out by the above background technology, an embodiment of the present invention provides a microscopic sample preparation device and a cell phenotype control device.

[0005] According to one aspect of an embodiment of the present invention, there is provided a microscopic sample preparation device, comprising: a molding plate, having a plurality of molding holes penetrating two opposite surfaces of the molding plate, for molding a plurality of solid culture media; a sample pool plate, having a plurality of sample holes penetrating two opposite surfaces of the sample pool plate, for accommodating a plurality of the solid culture media; a cover glass for being placed on one surface of the sample pool plate; and a pressing plate, provided with a plurality of pressing columns, for pressing the plurality of solid culture media in the plurality of the molding holes into the plurality of the sample holes and onto the cover glass.

[0006] In some embodiments, it also includes: a first glass sheet for being placed on the bottom surface of the molding plate; a baffle ring for being placed on the top surface of the molding plate to form a pouring trough for pouring liquid culture medium together with the top surface of the molding plate, and the pouring trough is connected to the plurality of molding holes; a second glass sheet for being placed on the top surface of the molding plate after removing the baffle ring to mold the solid culture medium together with the first glass plate and the molding holes.

[0007] In some embodiments, the microscopic sample preparation device also includes: a storage box, including a storage cavity with an open top for placing the molding plate, a bottom support frame for supporting the molding plate, and a side limiting frame for limiting the outer periphery of the molding plate.

[0008] In some embodiments, it also includes: a pressure ring for pressing the cover glass against the surface of the sample pool plate.

[0009] In some embodiments, the pressure ring and the sample pool plate are fixed by magnetic attraction.

[0010] In some embodiments, it also includes: a black sticky pad for sticking the cover glass to the surface of the sample pool plate, the sticky pad having a plurality of openings penetrating two opposite surfaces of the sticky pad, and the plurality of openings are used to correspond one-to-one with the plurality of sample holes.

[0011] In some embodiments, it also includes: a positioning tray, including a accommodating cavity with an open top for placing the sample pool plate, a middle supporting protrusion for supporting the cover glass and the sample pool plate, and an outer limiting protrusion for limiting the outer periphery of the cover glass and the sample pool plate.

[0012] In some embodiments, it also includes: a drying rack, having at least one left support block and at least one right support block, the left support block and the right support block are arranged at intervals on the left and right and correspond to each other, and the left support block and the right support block are respectively provided with slots for inserting the forming plate on the sides facing each other.

[0013] In some embodiments, the pressing plate is further provided with a handle, and the handle and the pressing column are respectively provided on two opposite surfaces of the pressing plate.

[0014] In some embodiments, it also includes: a moisturizing plate, provided with a plurality of through holes, which are used to connect with the plurality of sample holes one by one, a water storage tank is provided on one surface of the moisturizing plate, the plurality of through holes are connected to the water storage tank, and the plurality of through holes are separated from the water storage tank by a flow-blocking ring wall; a transparent cover plate, used to cover the surface of the moisturizing plate to close the water storage tank.

[0015] In some embodiments, it also includes: an intermediate connecting plate, which is provided with a plurality of through holes corresponding one-to-one to the plurality of forming holes, and the intermediate connecting plate is used to detachably connect the forming plate and the cell culture plate of the cell phenotype control device.

[0016] According to another aspect of an embodiment of the present invention, a cell phenotype control device is provided, comprising a cell culture plate and the microscopic sample preparation device of the aforementioned embodiment.

[0017] The beneficial effects of the embodiments of the present invention include at least:

[0018] 1. The embodiment of the present invention can prepare multiple solid culture media at one time and complete the tableting operation of multiple samples at one time by providing a forming plate with multiple forming holes, a sample pool plate with multiple sample holes and a pressing plate with multiple pressing columns, thereby saving time and effectively improving the experimental efficiency. The operation is simple, convenient and fast, and it is not easy to make operating errors or crush the cover glass.

[0019] 2. In the embodiment of the present invention, the first glass sheet and the second glass sheet are arranged to form the solid culture medium together with the forming plate, thereby ensuring that the surface of the solid culture medium is flat, and providing a culture medium with a higher surface quality for the drop suspension;

[0020] 3. The storage box in the embodiment of the present invention can store liquid culture medium, provide good support for the forming plate when preparing solid culture medium, and also play a role in positioning and aligning the forming plate, the first glass sheet and the second glass sheet;

[0021] 4. In the embodiment of the present invention, a drying rack is provided to basically dry the solid culture medium before dropping the suspension, thereby shortening the drying time during the dropping of the suspension, preventing a large amount of water from being generated during the subsequent tableting process, and thus preventing the microorganisms in the suspension from migrating with the water and failing to meet the experimental requirements;

[0022] 5. The embodiment of the present invention provides a moisturizing member to moisturize the solid culture medium in the sample pool plate, thereby preventing the solid culture medium from dehydrating and deforming and separating from the cover glass, thereby facilitating the experimenter to perform microscopic observation for a long time;

[0023] 6. In the embodiment of the present invention, a middle connecting plate is provided to detachably connect the forming plate and the cell culture plate, thereby forming a set of cell phenotype control devices that are easy to assemble and disassemble, so as to facilitate optogenetic experiments.

[0024] With reference to the following description and accompanying drawings, specific embodiments of the present invention are disclosed in detail, indicating the manner in which the principles of the present invention can be adopted. It should be understood that the embodiments of the present invention are not limited in scope. Within the spirit and scope of the appended claims, the embodiments of the present invention include many changes, modifications and equivalents. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] The included drawings are used to provide a further understanding of the embodiments of the present invention, which constitute a part of the specification, are used to illustrate the implementation of the present invention, and together with the text description, explain the principles of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. In the drawings:

[0026] Figure 1is a schematic diagram of an example of some components of the microscopic sample preparation device according to an embodiment of the present invention;

[0027] Figure 2 Schematic diagram of an example of a molding plate of a microscopic sample preparation device according to an embodiment of the present invention;

[0028] Figure 3 is a schematic diagram of an example of a sample pool plate of a microscopic sample preparation device according to an embodiment of the present invention;

[0029] Figure 4 Schematic diagram of an example of a flow barrier of a microscopic sample preparation device according to an embodiment of the present invention;

[0030] Figure 5 is a schematic diagram of an example of a storage box of a microscopic sample preparation device according to an embodiment of the present invention;

[0031] Figure 6 is a schematic diagram of an example of a sample preparation component of a microscopic sample preparation device according to an embodiment of the present invention;

[0032] Figures 7 to 10 It is a schematic diagram of the coordination of components of the microscopic sample preparation device in the sample preparation process according to an embodiment of the present invention;

[0033] Fig.11 yes Fig.10 A cross-sectional view of the components in FIG.

[0034] Fig.12 and Fig.13 Schematic diagram of an example of a drying rack of a microscopic sample preparation device according to an embodiment of the present invention;

[0035] Figures 14 to 16 Schematic diagram of an example of a sample pool plate assembly of a microscopic sample preparation device according to an embodiment of the present invention;

[0036] Fig.17 is a schematic diagram of a sticky pad of a microscopic sample preparation device according to an embodiment of the present invention being placed between a sample pool plate and a cover glass;

[0037] Fig.18 is a schematic diagram of an example of a positioning tray of a microscopic sample preparation device according to an embodiment of the present invention;

[0038] Fig.19 and Fig. 20 is a schematic diagram of placing a sample pool plate assembly of a microscopic sample preparation device according to an embodiment of the present invention on a positioning tray;

[0039] Fig.21 and Fig. 22 It is a schematic diagram of placing a sample pool plate assembly and a molding plate of a microscopic sample preparation device according to an embodiment of the present invention on a positioning tray;

[0040] Fig.23 Schematic diagram of an example of a pressing plate of a microscopic sample preparation device according to an embodiment of the present invention;

[0041] Fig.24 and Fig.25 It is a schematic diagram of the coordination of components of the microscopic sample preparation device according to an embodiment of the present invention during the tablet pressing process;

[0042] Fig.26 and Fig. 27 This is a schematic diagram of the coordination of components of the microscopic sample preparation device according to an embodiment of the present invention after tableting;

[0043] Fig.28 Schematic diagram of an example of a moisturizing member of a microscopic sample preparation device according to an embodiment of the present invention;

[0044] Fig.29 and Fig.30 It is a schematic diagram of the cooperation between the moisturizing member and the sample pool plate of the microscopic sample preparation device according to an embodiment of the present invention;

[0045] Fig.31 Schematic diagram of an example of a central connecting plate of a microscopic sample preparation device according to an embodiment of the present invention;

[0046] Fig.32 and Fig.33 Schematic diagram of an example of a cell phenotype control device according to an embodiment of the present invention.

[0047] Description of main component numbers:

[0048] 101, forming plate; 102, forming hole; 103, sample pool plate; 104, cover glass; 105, pressing plate;

[0049] 106, sample hole; 107, pressure column; 108, handle; 109, positioning pin installation hole; 110, positioning hole;

[0050] 111, first glass sheet; 112, baffle; 113, second glass sheet; 114, first magnet mounting hole;

[0051] 115, second magnet mounting hole; 116, storage box; 117, storage cavity; 118, bottom support frame;

[0052] 119, side limit frame; 120, operation avoidance groove; 121, pressure ring; 122, third magnet mounting hole;

[0053] 123, limiting ridge; 124, positioning tray; 125, accommodating cavity; 126, middle supporting protrusion;

[0054] 127, outer limiting protrusion; 128, drying rack; 129, left support block; 130, right support block;

[0055] 131. slot; 132. lower support plate; 133. upper support plate; 134. column; 135. moisturizing member;

[0056] 136, moisturizing plate; 137, transparent cover plate; 138, through hole; 139, water storage tank; 140, flow-blocking ring wall;

[0057] 141. middle connecting plate; 142. through hole; 143. sticky pad; 144. positioning pin;

[0058] 200. solid culture medium; 300. cell culture plate; 301. microwell; 400. light source control box. DETAILED DESCRIPTION

[0059] With reference to the accompanying drawings, the above and other features of the present invention will become apparent through the following description. In the description and the accompanying drawings, specific embodiments of the present invention are specifically disclosed, which show some embodiments in which the principles of the present invention can be adopted. It should be understood that the present invention is not limited to the described embodiments, but rather, the present invention includes all modifications, variations and equivalents falling within the scope of the appended claims.

[0060] In the embodiments of the present invention, the terms "first", "second", etc. are used to distinguish different elements in terms of name, but do not indicate the spatial arrangement or time order of these elements, etc., and these elements should not be limited by these terms. The term "and / or" includes any one and all combinations of one or more of the associated listed terms. The terms "comprising", "including", "having", etc. refer to the existence of the stated features, elements, components or components, but do not exclude the existence or addition of one or more other features, elements, components or components.

[0061] In the embodiments of the present invention, the singular forms "a", "the", etc. may include plural forms and should be broadly understood as "a kind" or "a type" rather than being limited to the meaning of "one"; in addition, the term "said" should be understood to include both singular and plural forms, unless the context clearly indicates otherwise; in addition, the term "according to" should be understood as "at least partially based on...", and the term "based on" should be understood as "at least partially based on...", unless the context clearly indicates otherwise; in addition, the term "plurality" means two or more, unless otherwise specified.

[0062] In an embodiment of the present invention, the use of the adjective or adverb modifiers "up" and "down", "top" and "bottom", "inside" and "outside", "front" and "back" is only for the convenience of relative reference between multiple groups of terms, and does not describe any specific directional limitation on the modified terms.

[0063] The following describes implementation modes of the embodiments of the present invention with reference to the accompanying drawings.

[0064] Embodiments of the first aspect

[0065] An embodiment of the first aspect of the present invention provides a microscopic sample preparation device.

[0066] The microscopic sample preparation device of the embodiment of the present invention comprises a sample preparation component and a tablet pressing component, such as Figure 1 As shown, the sample preparation component includes a molding plate 101, and the molding plate 101 has a plurality of molding holes 102 respectively penetrating two opposite surfaces of the molding plate 101, and the plurality of molding holes 102 are used to mold a plurality of solid culture media 200 (such as Fig.11 As shown), a plurality of solid culture media 200 are prepared at a time, for example, the solid culture media 200 is an agar block; Fig.14 , Fig.23 As shown, the pressing assembly includes a sample pool plate 103, a cover glass 104 and a pressing plate 105. The sample pool plate 103 has a plurality of sample holes 106 that pass through two opposite surfaces of the sample pool plate 103. The plurality of sample holes 106 can correspond one-to-one to the plurality of forming holes 102. The cover glass 104 is used to be placed on a surface of the sample pool plate 103. The pressing plate 105 is provided with a plurality of pressing columns 107. The plurality of pressing columns 107 can correspond one-to-one to the plurality of forming holes 102, so as to press the plurality of solid culture media 200 in the plurality of forming holes 102 into the plurality of sample holes 106 and onto the cover glass 104. For example, a handle 108 is further provided on the pressing plate 105. The handle 108 and the pressing column 107 are respectively arranged on the opposite side surfaces of the pressing plate 105, so that the pressing plate 105 can be pressed down by holding the handle 108.

[0067] The sample preparation using the microscopic sample preparation device of this embodiment may include the following steps:

[0068] Step S100: Prepare solid culture medium (e.g. agar block): Fig.11 As shown, a liquid culture medium (such as agar solution) is poured into a plurality of molding holes 102 of a molding plate 101, and the liquid culture medium is cooled and solidified to become a solid culture medium 200;

[0069] Step S200: dripping: dropping the bacterial / cell suspension onto the surface (called the front side) of a plurality of solid culture media 200;

[0070] Step S300: tableting: Figure 24 to Figure 27As shown, the cover glass 104 is placed on the bottom surface of the sample pool plate 103, the molding plate 101 is placed on the top surface of the sample pool plate 103 (the front of the solid culture medium 200 faces downward), and the pressing plate 105 is placed on the molding plate 101. At this time, the pressing column 107, the molding hole 102 and the sample hole 106 correspond one by one from top to bottom. The pressing plate 105 is pressed down until the pressing column 107 presses the multiple solid culture media 200 in the multiple molding holes 102 into the multiple sample holes 106 and onto the cover glass 104, so that multiple microscopic samples of bacteria / cell suspension droplets in contact with the cover glass 104 can be obtained. Subsequently, the sample pool plate 103 can be placed on the microscope platform, and the suspension droplets on the solid culture medium 200 can be observed through the cover glass 104.

[0071] The microscopic sample preparation device of this embodiment can be used to prepare multiple solid culture media at one time and complete the tableting operations of multiple samples at one time, saving time and effectively improving experimental efficiency. The operation is simple and convenient, and it is not easy to make operating errors or crush the cover glass.

[0072] Furthermore, the height of the pressing column 107 should not be less than the height of the forming hole 102 , so as to press the solid culture medium 200 out of the forming hole 102 and into the sample hole 106 .

[0073] Furthermore, the aperture of the sample hole 106 is larger than the aperture of the forming hole 102 to prevent the edge of the solid culture medium 200 from contacting the hole wall of the sample hole 106 and being damaged when the solid culture medium 200 is pressed into the sample hole 106, and also to prevent the residual gas under the solid culture medium 200 pressed into the sample hole 106 from causing pressure buildup.

[0074] In some embodiments, Figure 2 , Figure 3 and Fig.23 As shown, the forming plate 101, the sample pool plate 103 and the pressing plate 105 can all be square plates, and the length and width of the forming plate 101, the sample pool plate 103 and the pressing plate 105 can be the same. The multiple forming holes 102 on the forming plate 101 can be arranged in multiple rows and columns, such as 8 rows and 12 columns, that is, the number of forming holes 102 can be 96, and the arrangement and number of the sample holes 106 and the arrangement and number of the pressing columns 107 are consistent with the arrangement and number of the forming holes 102, so as to form a one-to-one correspondence.

[0075] Further, if Figure 2 As shown, hole position marks can be set on the two vertical sides of the molding plate 101, such as numerical characters (such as 1 to 12) in the length direction and alphabetical characters (such as A to H) in the width direction, so as to facilitate the description of the position of each molding hole 102, that is, to facilitate the description of the position of multiple samples.

[0076] Further, if Figure 3 , Fig. 20 As shown, positioning pin mounting holes 109 can be respectively provided at two opposite sides of the sample pool plate 103 to fix the positioning pins 144. Figure 2 , Fig. 20 , Fig.21 As shown, positioning holes 110 can be respectively provided at two opposite side plates of the molding plate 101 to be plugged with the positioning pins 144 for positioning, so that in the tableting step of step S300, the relative positions of the sample pool plate 103 and the molding plate 101 are positioned so that the two are centered and aligned.

[0077] In some embodiments, Figure 6 As shown, the sample preparation component may also include a first glass sheet 111, a baffle 112 and a second glass sheet 113. For example, the first glass sheet 111 and the second glass sheet 113 are ultra-thin glasses with a thickness of less than 0.2 mm. The first glass sheet 111 is used to be placed on the bottom surface of the molding plate 101 to close the lower end of the molding hole 102. The baffle 112 is used to be placed on the top surface of the molding plate 101, that is, the baffle 112 surrounds the outside of the multiple molding holes 102 to form a pouring trough for pouring liquid culture medium together with the top surface of the molding plate 101, and the pouring trough is connected to the multiple molding holes 102; the second glass sheet 113 is used to be placed on the top surface of the molding plate 101 after the baffle 112 is removed, so as to mold the sample together with the first glass plate and the molding hole 102, so as to ensure that the surface of the solid culture medium is flat, and provide a culture medium with a higher surface quality for the dripping suspension.

[0078] When the microscopic sample preparation device of this embodiment is used to prepare a sample, in the step of preparing a solid culture medium in step S100, the following steps may be specifically included:

[0079] Step S110: Figure 7 , Figure 8 As shown, a first glass sheet 111 is placed on the bottom surface of the forming plate 101, and a baffle 112 is placed on the top surface of the forming plate 101;

[0080] Step S120: pouring the liquid culture medium into the pouring trough inside the baffle 112 (for example, using a large-volume pipette), the liquid culture medium flows from the pouring trough into the multiple forming holes 102, wherein the injection amount of the liquid culture medium needs to ensure that after the multiple forming holes 102 are filled with the liquid culture medium, the liquid level of the liquid culture medium is still higher than the top surface of the forming plate 101. After the pouring is completed, observe whether each forming hole 102 is filled with the liquid culture medium. If it is not full, gently shake the forming plate 101 to evenly distribute the liquid culture medium to ensure that the multiple forming holes 102 are filled with the liquid culture medium.

[0081] Step S130: After the plurality of forming holes 102 are filled with liquid culture medium, the flow blocking ring 112 is removed from the top surface of the forming plate 101;

[0082] Step S140: Fig. 9 As shown, the second glass sheet 113 is slowly lowered against one side of the molding plate 101, so that the second glass sheet 113 contacts and soaks the liquid culture medium on the top surface of the molding plate 101, ensuring that there are no bubbles, until the second glass sheet 113 is entirely attached to the top surface of the molding plate 101;

[0083] Step S150: Fig.10 , Fig.11 As shown, the second glass sheet 113 is pressed downward evenly to squeeze out a layer of liquid culture medium remaining between the second glass sheet 113 and the top surface of the molding plate 101, so that as little liquid culture medium as possible remains between the molding plate 101 and the second glass sheet 113, so as to ensure that both end surfaces of the solid culture medium 200 molded in each molding hole 102 are flat;

[0084] Step S160: After the liquid culture medium in the molding hole 102 is cooled and solidified, the residual agar on the edge of the molding plate 101 is cleaned, and then the first glass sheet 111 and the second glass sheet 113 are respectively pushed away along the bottom and top surfaces of the molding plate 101 to obtain a cylindrical solid culture medium 200 with flat end surfaces.

[0085] Furthermore, the baffle 112 and the forming plate 101 can be fixed and attached by magnetic attraction, and the assembly and disassembly is very convenient. Figure 4 As shown, at least two opposite sides of the baffle ring 112 are provided with first magnet mounting holes 114 for mounting the first magnet; Figure 2 As shown, second magnet mounting holes 115 are provided at at least two opposite side edges of the molding plate 101 for mounting a second magnet, the second magnet corresponds to the first magnet, and when the baffle ring 112 is placed on the top surface of the molding plate 101, the first magnet and the second magnet are magnetically attracted to make the baffle ring 112 and the molding plate 101 fit and fix, and the magnet and the magnet mounting hole can be fixed by interference fit.

[0086] In order to facilitate the removal of the baffle ring 112, side ears (such as Figure 4 , Figure 8 As shown in FIG. 1 , when the spoiler 112 is placed on the top surface of the molding plate 101 , the two side ears are located on the outside of the molding plate 101 rather than being in contact with the molding plate 101 .

[0087] Furthermore, the shapes of the first glass sheet 111 , the baffle ring 112 , and the second glass sheet 113 may all be squares that are consistent with the shape of the forming plate 101 .

[0088] In some embodiments, Figure 5 As shown, the microscopic sample preparation device may further include a storage box 116, which is used to support and position the molding plate 101 when preparing the solid culture medium 200, and to store the overflowed liquid culture medium. The storage box 116 has a storage cavity 117 with an open top for placing the molding plate 101, a bottom support frame 118 for supporting the molding plate 101, and a side limiting frame 119 for limiting the outer periphery of the molding plate 101. Figure 5 In the example, the bottom support frame 118 and the side limit frame 119 are both arranged in the storage cavity 117. For example, the bottom support frame 118 and the side limit frame 119 are both rib plates. An operation avoidance groove 120 can be set on the outer wall of the storage box 116 to facilitate the placement of components such as the molding plate 101.

[0089] When the microscopic sample preparation device of this embodiment is used to prepare a sample, in step S110, as shown in FIG. Figures 6 to 8 As shown, the first glass sheet 111, the forming plate 101 and the baffle ring 112 can be stacked from bottom to top on the bottom support frame 118 in the storage cavity 117, and the side limit frame 119 limits the horizontal position of the first glass sheet 111, the forming plate 101 and the baffle ring 112 so that the three are centered and aligned. Fig. 9 As shown, when the second glass sheet 113 is pressed down, the squeezed liquid culture medium flows into the receiving cavity 117 .

[0090] In some embodiments, Figures 14 to 16 As shown, the microscope sample preparation device may further include a pressure ring 121, and the pressure ring 121 is used to press the cover glass 104 against the surface of the sample pool plate 103 so that the cover glass 104 and the surface of the sample pool plate 103 fit tightly.

[0091] When preparing samples using the microscope sample preparation device of this embodiment, in the pressing step of step S300, the cover glass 104 can be placed on the bottom surface of the sample pool plate 103, and then the pressure ring 121 can be placed on the bottom surface of the cover glass 104 to fix the cover glass 104 and the sample pool plate 103 through the pressure ring 121.

[0092] In a feasible technical solution, the pressure ring 121 is fixed to the sample pool plate 103 by magnetic attraction. Specifically, the pressure ring 121 is made of metal that can be attracted by a magnet, and at least two opposite sides of the sample pool plate 103 are provided with a third magnet mounting hole 122 (such as Figure 3 As shown), it is used to install the third magnet. When the pressure ring 121 is placed on the surface of the cover glass 104 facing away from the sample pool plate 103, the third magnet and the pressure ring 121 are magnetically attracted (as shown in FIG. Fig.15 As shown), the cover glass 104 and the sample pool plate 103 can fit tightly together and be easily assembled and disassembled.

[0093] Further, if Figure 3 As shown, two limiting ridges 123 can be set on the surface of the sample pool plate 103 for mounting the cover glass 104. The two limiting ridges 123 are respectively arranged at two opposite sides of the sample pool plate 103. On the one hand, they limit the cover glass 104 to the left and right, and on the other hand, they limit the pressure ring 121 to the left and right.

[0094] In other embodiments, Fig.17 As shown, the microscope sample preparation device can also include a black sticky pad 143, which is used to stick the cover glass 104 to the surface of the sample pool plate 103. The sticky pad 143 has a plurality of openings that penetrate the two opposite surfaces of the sticky pad 143. When the sticky pad 143 is stuck to the surface of the sample pool plate 103, the plurality of openings are connected to the plurality of sample holes 106 in a one-to-one correspondence.

[0095] Compared with the fixing method of the pressure ring in the previous embodiment, the sticky pad 143 of this embodiment is all over the sample pool plate 103, and can be well attached to the cover glass 104 and the sample pool plate 103 with a smooth surface. The sticky pad 143 is easily separated from the surface of the cover glass 104 and the surface of the sample pool plate 103 after encountering water, and can be repeatedly pasted. The thickness of the sticky pad 143 should be as thin as possible and the color should be black, so that no light can leak between the holes when performing optogenetic experiments.

[0096] However, the present invention is not limited thereto, and the cover glass 104 and the sample pool plate 103 may also be fixed by other existing connection methods that are easy to disassemble and assemble.

[0097] In some embodiments, Fig.18 As shown, the microscopic sample preparation device may further include a positioning tray 124, the positioning tray 124 includes a receiving cavity 125 with an open top for placing the sample pool plate 103, a middle supporting protrusion 126 for supporting the cover glass 104 and the sample pool plate 103, and an outer limiting protrusion 127 for limiting the outer periphery of the cover glass 104 and the sample pool plate 103. When the sample pool assembly consisting of the cover glass 104, the sample pool plate 103 and the pressure ring 121 is placed on the middle supporting protrusion 126 (as shown in FIG. Fig.19 , Fig. 20 As shown in FIG. 1 , the pressure ring 121 is suspended outside the middle support protrusion 126, rather than being fitted to the middle support protrusion 126. The surface of the middle support protrusion 126 is a plane, so as to provide a good support effect for the cover glass 104.

[0098] In the tablet pressing step of step S300, the sample pool assembly can be placed on the middle support protrusion 126 of the positioning tray 124 with the pressing ring 121 facing downward (eg, Figures 18 to 20Then, the forming plate 101 is placed on the top surface of the sample pool plate 103 with the front side of the solid culture medium 200 facing downward (as shown in FIG. Fig.21 , Fig. 22 As shown in the figure, the outer limiting protrusion 127 of the positioning tray 124 limits the outer periphery of the sample pool assembly and the molding plate 101, so that the sample pool plate 103 and the molding plate 101 are aligned to prevent the two from being misaligned. Since the cover glass 104 is supported by the middle supporting protrusion 126, when the pressing plate 105 is pressed down, the cover glass 104 is not easily deformed or damaged.

[0099] In some embodiments, Fig.12 As shown, the microscopic sample preparation device can also include a drying rack 128 for drying the solid culture medium 200 on the molding plate 101. The drying rack 128 has at least one left support block 129 and at least one right support block 130. The left support block 129 and the right support block 130 are arranged at intervals on the left and right and correspond to each other. The left support block 129 and the right support block 130 are respectively provided with slots 131 for inserting the molding plate 101 on the side facing each other, so that the molding plate 101 can be dried on the drying rack 128 in a suspended state to prevent the solid culture medium 200 in the molding hole 102 from contacting the drying rack 128 and being deformed.

[0100] When the microscopic sample preparation device of this embodiment is used to prepare a sample, in the step of preparing a solid culture medium in step S100, a step S170 may also be included: Fig.13 As shown, the forming plate 101 is placed on the drying rack 128 to dry the solid culture medium 200. After the solid culture medium 200 is basically dried, the suspension is dripped on the solid culture medium 200, thereby shortening the drying time during the process of dripping the suspension, preventing a large amount of water from being generated during the subsequent tableting process, and thus preventing the microorganisms in the suspension from migrating with the water and failing to meet the experimental requirements.

[0101] Further, if Fig.12 , Fig.13 As shown, the drying rack 128 can have multiple left support blocks 129 and multiple right support blocks 130. The multiple left support blocks 129 are arranged at intervals in the height direction of the drying rack 128. Correspondingly, the multiple right support blocks 130 are arranged at intervals in the height direction of the drying rack 128 and correspond one-to-one with the multiple left support blocks 129 in the horizontal direction.

[0102] Further, if Fig.12 , Fig.13As shown, the drying rack 128 also includes a lower support plate 132, an upper support plate 133, and a plurality of columns 134 (such as copper columns) connecting the lower support plate 132 and the upper support plate 133. For example, the lower support plate 132 and the upper support plate 133 are both square hollow frames, and there are four columns 134, which are respectively connected and supported from the four corners of the lower support plate 132 and the upper support plate 133. The left support block 129 and the right support block 130 can be fixed on the columns 134.

[0103] In some embodiments, Fig.28 , Fig.29 , Fig.30 As shown, the microscopic sample preparation device can also include a moisturizing component 135, which includes a moisturizing plate 136 and a transparent cover plate 137. The moisturizing plate 136 has a plurality of through holes 138 that penetrate two opposite surfaces of the moisturizing plate 136, and the plurality of through holes 138 are used to be connected with the plurality of sample holes 106 in a one-to-one correspondence. A water storage tank 139 is provided on one surface of the moisturizing plate 136, and the plurality of through holes 138 are connected with the water storage tank 139 to allow water vapor in the water storage tank 139 to enter the through holes 138. The plurality of through holes 138 are separated from the water storage tank 139 by a blocking ring wall 140 to limit the water in the water storage tank 139 from flowing into the through holes 138; the transparent cover plate 137 can be a glass cover plate, which is used to cover the surface of the moisturizing plate 136 to close the water storage tank 139.

[0104] During the experiment, when the microscopic sample needs to be observed for a long time, the moisturizing member 135 can be used to moisturize the solid culture medium 200 in the sample pool plate 103 to prevent the solid culture medium 200 from separating from the cover glass 104 due to dehydration and deformation (such as shrinking toward the middle).

[0105] When the microscopic sample preparation device of this embodiment is used to prepare a sample, the method may further include step S400: Fig.29 , Fig.30 As shown, the lower surface of the moisturizing plate 136 facing away from the water reservoir 139 is placed in contact with the surface of the sample pool plate 103 facing away from the cover glass 104, with the water reservoir 139 facing upward. Water is added to the water reservoir 139, and then the transparent cover plate 137 is covered on the upper surface of the moisturizing plate 136 to seal the water reservoir 139 to obtain a closed moisturizing space. The water in the water reservoir 139 evaporates, so that a certain humidity is maintained in the moisturizing space, thereby effectively preventing the solid culture medium 200 in the sample pool plate 103 from dehydration.

[0106] In addition, since the multiple through holes 138 correspond one-to-one to the multiple sample holes 106 and the transparent cover 137 is made of transparent material, it can ensure that the light source above the microscope device can smoothly illuminate the solid culture medium 200 without affecting the normal progress of the optical experiment.

[0107] In some embodiments, Fig.31 As shown, the microscopic sample preparation device also includes a middle connecting plate 141, which is provided with a plurality of through holes 142 corresponding to the plurality of forming holes 102 one by one. The middle connecting plate 141 is used to detachably connect the forming plate 101 and the cell culture plate 300 of the cell phenotype control device (such as Fig.32 As shown in the figure, the microscopic sample preparation device and the cell phenotype control device form a set of devices, which are easy to assemble and disassemble. When the molding plate 101 and the cell culture plate 300 are connected through the intermediate plate 141, the multiple molding holes 102 on the molding plate 101, the multiple sample holes 106 of the sample pool plate 103, the multiple through holes 142 on the intermediate plate 141 and the multiple microholes 301 on the cell culture plate 300 correspond to each other.

[0108] The intermediate connecting plate 141 and the forming plate 101 can be connected by magnetic attraction, and the intermediate connecting plate 141 and the cell culture plate 300 can be connected by interference fit. For example, two opposite sides of the intermediate connecting plate 141 are provided with magnet mounting holes for mounting a fourth magnet, and the fourth magnet corresponds to the second magnet of the forming plate 101, so as to fix the intermediate connecting plate 141 and the forming plate 101 by magnetic attraction.

[0109] Embodiments of the second aspect

[0110] The second aspect of the present invention provides a cell phenotype control device, such as Fig.32 , Fig.33 As shown, the cell phenotype control device includes a cell culture plate 300, a light source control box 400 and the microscopic sample preparation device described in the embodiment of the first aspect. Since the structure of the microscopic sample preparation device has been described in detail in the embodiment of the first aspect, its content is incorporated here and the description is omitted here.

[0111] In the embodiment of the present invention, for other structures of the cell phenotype control device, reference may be made to the relevant art, and the description is omitted here. For example, reference may be made to other structures of the cell phenotype control device in the Chinese invention patent application with patent application number CN202011476070.6.

[0112] The present invention has been described above in conjunction with specific embodiments, but it should be clear to those skilled in the art that these descriptions are exemplary and are not intended to limit the scope of protection of the present invention. Those skilled in the art may make various modifications and variations to the present invention based on the spirit and principles of the present invention, and these modifications and variations are also within the scope of the present invention.

[0113] The preferred embodiments of the present invention have been described above with reference to the accompanying drawings. Many features and advantages of these embodiments are clear from this detailed description, and therefore the appended claims are intended to cover all such features and advantages of these embodiments that fall within their true spirit and scope. In addition, since many modifications and changes are readily apparent to those skilled in the art, the embodiments of the present invention are not intended to be limited to the precise structure and operation illustrated and described, but all suitable modifications and equivalents that fall within their scope may be included.

Claims

1. A microscopic sample preparation device, characterized in that: include: A forming plate having a plurality of forming holes penetrating through two opposite surfaces of the forming plate, for forming a plurality of solid culture media; A sample pool plate, having a plurality of sample holes penetrating two opposite surfaces of the sample pool plate, for accommodating a plurality of the solid culture media, wherein the aperture of the sample holes is larger than the aperture of the forming holes; A cover glass, used for being placed on a surface of the sample pool plate; A pressing plate, provided with a plurality of pressing columns, for pressing the plurality of solid culture media in the plurality of the forming holes into the plurality of the sample holes and onto the cover glass; A first glass sheet, used for being placed on the bottom surface of the forming plate; A baffle ring, used for being placed on the top surface of the molding plate, so as to enclose together with the top surface of the molding plate a pouring trough for pouring liquid culture medium, wherein the pouring trough is connected to the plurality of molding holes; A second glass sheet, used to be placed on the top surface of the molding plate after the blocking ring is removed, so as to mold the solid culture medium together with the first glass sheet and the molding hole; The sample preparation using the microscopic sample preparation device comprises the following steps: Step S100, preparing a solid culture medium: pouring a liquid culture medium into a plurality of forming holes of a forming plate, and the liquid culture medium becomes a solid culture medium after being cooled and solidified; Step S200: Dropping: dropping the bacterial / cell suspension onto the surface of multiple solid culture media, called the front side; Step S300: Pressing: Place a cover glass on the bottom surface of the sample pool plate, place a molding plate on the top surface of the sample pool plate, with the front of the solid culture medium facing downward, and place a pressing plate on the molding plate. At this time, the pressing columns, molding holes and sample holes correspond one by one from top to bottom. Press the pressing plate downward until the pressing columns press the multiple solid culture media in the multiple molding holes into the multiple sample holes and onto the cover glass, so that multiple microscopic samples of bacteria / cell suspension droplets in contact with the cover glass can be obtained. Subsequently, the sample pool plate can be placed on a microscope platform, and the suspension droplets on the solid culture medium can be observed through the cover glass.

2. The microscopic sample preparation device according to claim 1, characterized in that: The microscopic sample preparation device also includes: The storage box comprises a storage cavity with an open top for placing the forming board, a bottom support frame for supporting the forming board, and a side limiting frame for limiting the outer periphery of the forming board.

3. The microscopic sample preparation device according to claim 1, characterized in that: Also includes: A pressure ring is used to press the cover glass onto the surface of the sample pool plate.

4. The microscopic sample preparation device according to claim 3, characterized in that: The pressure ring and the sample pool plate are fixed by magnetic attraction.

5. The microscopic sample preparation device according to claim 1, characterized in that: Also includes: The black sticky pad is used to stick the cover glass to the surface of the sample pool plate. The sticky pad has a plurality of openings penetrating two opposite surfaces of the sticky pad. The plurality of openings are used to correspond one-to-one with the plurality of sample holes.

6. The microscopic sample preparation device according to claim 1, characterized in that: Also includes: The positioning tray comprises a receiving cavity with an open top for placing the sample pool plate, a middle supporting protrusion for supporting the cover glass and the sample pool plate, and an outer limiting protrusion for limiting the outer periphery of the cover glass and the sample pool plate.

7. The microscopic sample preparation device according to claim 1, characterized in that: Also includes: The drying rack comprises at least one left support block and at least one right support block, wherein the left support block and the right support block are arranged at intervals on the left and right sides and correspond to each other, and slots for inserting the forming plates are respectively provided on the sides of the left support block and the right support block facing each other.

8. The microscopic sample preparation device according to claim 1, characterized in that: The pressing plate is also provided with a handle, and the handle and the pressing column are respectively provided on two opposite surfaces of the pressing plate.

9. The microscopic sample preparation device according to claim 1, characterized in that: Also includes: A moisturizing plate is provided with a plurality of through holes for correspondingly communicating with the plurality of sample holes one by one. A water storage tank is provided on one surface of the moisturizing plate. The plurality of through holes are connected with the water storage tank. The plurality of through holes are separated from the water storage tank by a flow-blocking ring wall. A transparent cover plate is used to cover the surface of the moisturizing plate to close the water storage tank.

10. The microscopic sample preparation device according to claim 1, characterized in that: Also includes: The middle connecting plate is provided with a plurality of through holes corresponding to the plurality of forming holes one by one, and the middle connecting plate is used to detachably connect the forming plate and the cell culture plate of the cell phenotype control device.

11. A cell phenotype control device, characterized in that: The invention comprises a microscopic sample preparation device and a cell culture plate as described in any one of claims 1 to 10.

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