A solid beverage containing a human aging BAZ2B gene inhibitor and a preparation method thereof

Abstract

The present application relates to a solid beverage containing a human aging BAZ2B gene inhibitor and a preparation method thereof. The yeast fermented solid beverage containing a BAZ2B gene inhibitor has the following components in parts by weight: 150-200 parts of calcium citrate; 100-150 parts of L-malate magnesium; 4-20 parts of bovine colostrum; 15-25 parts of L-serine; 40-60 parts of mucuna powder; 10-50 parts of trehalose; 2-6 parts of L-dopa, 0.01-0.05 parts of BAZ2B gene inhibitor; 20-30 parts of yeast extract, 0.5-1.5 parts of vitamin C; 0.01-0.02 parts of edible flavor; 0.01-0.05 parts of stevioside. The present application adds BAZ2B gene inhibitors and yeast extracts, so that the solid beverage can not only provide sufficient nutrients for new cells, but also delay aging by inhibiting the expression of the BAZ2B gene.

Description

Technical Field

[0001] The present application relates to the technical field of functional beverages, and more specifically, to a solid beverage containing a human aging BAZ2B gene inhibitor and a preparation method thereof. Background Art

[0002] As people's living standards improve, they pay more and more attention to their own health. Take functional solid beverages as an example. They appear in powder, granular or block form. They are popular among consumers because of their convenience and rich nutrients. They can provide the human body with sufficient vitamins, amino acids and energy to ensure the normal metabolism and growth and development of the human body. Long-term drinking is beneficial to maintaining human health.

[0003] Functional solid beverages in related technologies are solid products with a water content of no more than 5% made from fructose, dairy products, egg products or plant extracts as main raw materials, with appropriate amounts of nutrients added. After being taken, they can supplement the nutritional needs of the human body.

[0004] However, with the aging of the population, more and more elderly people are experiencing a significant decline in their behavioral and memory abilities due to aging, cerebrovascular diseases and other reasons. Some elderly people even suffer from Alzheimer's disease, which has brought a heavy burden to their families. Therefore, there is an urgent need to develop a functional beverage that can not only supplement the daily needs of the human body, but also delay aging, that is, improve the decline in behavior and memory. Summary of the invention

[0005] In order to solve the above technical problems, the present application provides a solid beverage containing a human aging BAZ2B gene inhibitor and a preparation method thereof.

[0006] In the first aspect, the present application provides a solid beverage containing a human aging BAZ2B gene inhibitor, using the following technical solution:

[0007] A solid beverage containing a human aging BAZ2B gene inhibitor, comprising the following components in parts by weight:

[0008] 150-200 parts of calcium citrate;

[0009] 100-150 parts of L-magnesium malate;

[0010] 4-20 servings of bovine colostrum;

[0011] 15-25 parts of L-serine;

[0012] 40-60 parts of Mucuna flour;

[0013] Trehalose 10-50 parts;

[0014] L-DOPA 2-6 parts;

[0015] BAZ2B gene inhibitor 0.01-0.05 parts;

[0016] Yeast extract 20-30 parts;

[0017] Vitamin C 0.5-1.5 parts;

[0018] 0.01-0.02 parts of edible flavor;

[0019] Stevioside 0.01-0.05 parts.

[0020] By adopting the above technical solution, the solid beverage with the above components and proportions can not only supplement basic nutrients for the human body, but also, by adding BAZ2B gene inhibitors and yeast extracts, give the solid beverage the effect of delaying human aging, improving behavioral ability and memory decline, and the effect is better after long-term drinking;

[0021] Among them, the BAZ2B gene inhibitor achieves the purpose of delaying aging, improving behavioral ability and memory decline by inhibiting the expression of the BAZ2B gene in the body. Yeast extract has a synergistic effect on the inhibition of the BAZ2B gene inhibitor. The selenium, amino acids, B vitamins, nucleotides, peptides, etc. in it can also promote the growth and development of new cells.

[0022] Analysis shows that the possible reason is that the BAZ2B gene is an important factor regulating the aging process. Its expression level is positively correlated with the progression of diseases such as Alzheimer's disease, and it regulates the functionality of mitochondria, which will accelerate the decline of mitochondrial function. Therefore, by inhibiting the expression of the BAZ2B gene, the function of aging cells and mitochondria in aging cells can be maintained, thereby achieving the effect of delaying aging. Experiments have shown that downregulation of the BAZ2B gene can significantly alleviate aging and improve memory degeneration and behavioral ability degeneration during the aging process.

[0023] Preferably, the yeast extract is a selenium-enriched yeast extract, and the selenium content in the selenium-enriched yeast extract is not less than 2000 mg / kg.

[0024] By adopting the above technical scheme, the selected selenium-enriched yeast is non-toxic and harmless, and has higher biological activity and food safety than inorganic selenium. The selenium-enriched yeast extract with the above selenium content can not only be used as a selenium nutritional additive to delay aging and regulate the blood selenium level in the human body, but can also be compounded with the BAZ2B gene inhibitor to enhance its inhibitory effect on the BAZ2B gene.

[0025] Preferably, the extraction method of the selenium-enriched yeast extract is as follows:

[0026] A. Yeast activation and expansion culture: First, pick beer yeast and inoculate it into the activation medium at a volume ratio of 1:(100-200) and culture it at a constant temperature of 30°C for 24-36 hours for activation. Then, transfer the activated yeast to the seed culture medium for expansion culture to obtain the activated seed liquid;

[0027] B. Selenium-enriched yeast fermentation culture: adding the activated seed liquid to the sterilized fermentation medium, and fermenting at 30-35° C. for 16-32 hours, during which the reducing sugar concentration in the fermentation medium is controlled to be not less than 1.2%, to obtain selenium-enriched yeast fermentation liquid;

[0028] The fermentation medium contains sodium selenite;

[0029] C. Preparation of selenium-enriched yeast extract:

[0030] (1) First, the selenium-enriched yeast fermentation liquid prepared in B is heated to 70-80° C., autolyzed for 0.5-1 h, then the temperature is adjusted to 40-50° C., the pH is adjusted to 6.0-7.0, and then an extraction enzyme is added. The amount of the extraction enzyme added is calculated based on the weight of the selenium-enriched yeast fermentation liquid, which is 100-160 mg of extraction enzyme / g of selenium-enriched yeast fermentation liquid. The hydrolysis reaction is carried out for 12-16 h to obtain a hydrolyzate;

[0031] The extraction enzyme is one or more of transglutaminase, nuclease, acid protease and papain;

[0032] (2) The hydrolyzate obtained in (1) is heated to 90-100° C., inactivated for 10-15 min, centrifuged at 3000-3500 r / min for 15-20 min, the supernatant is removed, and then powdered and dried to obtain the selenium-enriched yeast extract.

[0033] By adopting the above technical scheme, the selenium-enriched yeast extract obtained by the above preparation process is rich in selenium and has a high content of nutrients such as amino acids, B vitamins, nucleotides, and polypeptides, so it can better ensure the growth and development of new cells, and the selenium-enriched yeast extract has a synergistic effect on the inhibition of BAZ2B gene inhibitors.

[0034] Preferably, the activation medium used in A is YPD medium;

[0035] The seed culture medium used in A is composed of YPD culture medium and sodium selenite, and the weight ratio of the YPD culture medium to sodium selenite is 1:(0.001-0.002).

[0036] By adopting the above technical scheme, the seed culture medium within the above components and proportion range can increase the organic selenium content in yeast cells through primary screening without affecting the normal growth and development of yeast, thereby ensuring the retention of each effective ingredient in the yeast extract, enhancing its combined inhibitory effect with the BAZ2B gene inhibitor, and giving the solid beverage a better anti-aging and health-care effect.

[0037] Preferably, in B, the reducing sugar solution is added in a flowing manner to control the reducing sugar concentration in the fermentation medium to be not less than 1.2%;

[0038] The reducing sugar solution is composed of a glucose aqueous solution with a mass percentage concentration of 40-60% and sodium selenite in a weight ratio of 1:(0.2-0.3). The flow rate of the reducing sugar aqueous solution is 30-80 mg / h based on per liter of selenium-enriched yeast fermentation liquid.

[0039] By adopting the above technical scheme, the reducing sugar solution with the above flow addition amount and component ratio is not easy to affect the growth and development of selenium-enriched yeast. At the same time, it can ensure the nutrient supply for the normal proliferation and growth of yeast during the flow addition process, and has a certain screening effect, which is beneficial to the enrichment and proliferation of selenium-enriched yeast.

[0040] Preferably, the fermentation medium described in B, calculated by weight percentage, has the following raw material composition and content:

[0041] Yeast powder 3.5-4.5%

[0042] Soybean peptone 3.2-3.6%

[0043] Glucose 1.6-2.0%

[0044] Trehalose 1.0-1.6%

[0045] Adenine 0.005-0.01%

[0046] Sodium selenite 0.015-0.020%

[0047] The balance is water.

[0048] By adopting the above technical scheme, the fermentation medium with the above components and proportions can not only ensure the nutrient supply for the normal proliferation and growth of yeast during the fermentation process, but also has a screening effect, which is beneficial to the enrichment and proliferation of selenium-enriched yeast. In addition, the sodium selenite content is appropriate, and it is not easy to cause a large number of yeast deaths in the early stage of cultivation due to a high initial content, thereby affecting the fermentation effect.

[0049] Preferably, the extracting enzyme in C consists of transglutaminase, nuclease and papain.

[0050] By adopting the above technical scheme, the extraction enzymes of the above components have a better combination effect and can fully extract the effective ingredients in the yeast extract. Compared with using a single enzyme or other enzyme combinations, the compound effect of the yeast extract and the BAZ2B gene inhibitor is better.

[0051] Preferably, the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain in a weight ratio of 1: (0.5-0.8): (2-3).

[0052] By adopting the above technical scheme, the extraction enzyme with the above components and proportions has the best extraction effect on yeast extract, and can effectively extract selenium, amino acids, nucleic acids and other nutrients in the yeast extract, thereby ensuring the functional value of the yeast extract.

[0053] In a second aspect, the present application provides a method for preparing a solid beverage containing a human aging BAZ2B gene inhibitor, using the following technical solution:

[0054] S1. First, mix the components in the raw material with deionized water in a weight percentage of 1: (2-3), preferably 1: 2.5, then heat to 60-65 ° C and continue stirring, circulate and homogenize 3-5 times at a pressure of 50-60 MPa and a temperature of 60-65 ° C to obtain a mixed liquid;

[0055] S2. After sterilizing the mixed liquid prepared in S1, put it into the spray freeze dryer, and control the air inlet temperature to 120-140℃ and the air inlet flow rate to 200-260m 3 / h, the outlet air temperature is 80-100℃, preferably the inlet air temperature is controlled at 130℃, and the inlet air flow rate is 230m 3 / h, and the outlet air temperature is 90°C, so that a yeast fermented solid beverage containing BAZ2B gene inhibitor can be produced.

[0056] By adopting the above technical scheme, the process is simplified while various operating conditions are easy to control. The obtained yeast fermented solid beverage has a high retention of effective ingredients and a low water content. It is not easy to deteriorate or corrupt due to its own enzymes or microorganisms, which is conducive to long-term storage and transportation.

[0057] In summary, this application has the following beneficial effects:

[0058] 1. The present application adds BAZ2B gene inhibitor and yeast extract, so that the solid beverage can not only provide necessary nutrients for the human body, but also effectively delay aging, improve behavioral ability and memory decline. The reaction mechanism is as follows: by inhibiting the expression of BAZ2B gene, the function of senescent cells and mitochondria in senescent cells is maintained, thereby giving the solid beverage a better health care effect;

[0059] 2. The present application further ensures the efficacy of solid beverages in delaying aging and improving behavioral and memory decline by adding selenium-enriched yeast extract. The reason for this may be that the selenium element contained in it can not only be used as a selenium nutritional additive to delay aging and regulate the blood selenium level in the human body, but can also be compounded with the BAZ2B gene inhibitor to enhance its inhibitory effect on the BAZ2B gene;

[0060] 3. The preparation method in the present application is relatively simple, and the operating conditions are easy to control. The prepared solid beverage has a high retention of effective ingredients and is easy to store and transport, and is not prone to deterioration during storage, so it is suitable for industrial production. DETAILED DESCRIPTION

[0061] The present application is further described in detail below with reference to the embodiments.

[0062] Raw materials / equipment Specifications / Models Purchasing manufacturers BAZ2B gene inhibitors CAS 1619994-68-1 Shanghai Taoshu Biotechnology Co., Ltd. YPD medium Item No.: J903-100G Shanghai Aiyan Biotechnology Co., Ltd. Yeast powder CAS NO:8013-01-2 Hubei Honghan Biotechnology Co., Ltd. Brewer's yeast CMCC(F)98007 Beijing Boyan Kechuang Biotechnology Co., Ltd. Papain Product No.FDG-4003 Xiasheng (Beijing) Biotechnology Development Co., Ltd. Glutamine transaminase Product No.FDG-3901 Xiasheng (Beijing) Biotechnology Development Co., Ltd. Nuclease Product No.FDG-2253 Xiasheng (Beijing) Biotechnology Development Co., Ltd.

[0063] Note: The BAZ2B gene inhibitor in the above table is GSK2801

[0064] Preparation Example

[0065] Preparation Example 1-6

[0066] A fermentation medium, the components and corresponding weight parts are shown in the following table:

[0067] Table: Components and corresponding weights in Preparation Examples 1-6 (kg)

[0068]

[0069]

[0070] Preparation Example 7

[0071] A selenium-enriched yeast extract, the extraction method of which is as follows:

[0072] A. Yeast activation and expansion culture: After the beer yeast preserved in the glycerol tube is naturally thawed, 0.5 mL of the beer yeast is picked out and inoculated into a 500 mL conical flask containing 100 mL of activation culture medium at a volume ratio of 1:200, and cultured at a constant temperature of 30°C for 24 hours for activation. Then, the activated yeast is transferred to a culture tank containing 2 L of seed culture medium at a volume ratio of 1:20, and cultured at a constant temperature of 30°C for 36 hours for expansion to obtain an activated seed solution;

[0073] The activation medium used in A was YPD medium;

[0074] The seed culture medium used in A is composed of YPD culture medium and sodium selenite, and the weight ratio of YPD culture medium to sodium selenite is 1:0.0005;

[0075] B. Selenium-enriched yeast fermentation culture: add the activated seed liquid into 20L of sterilized fermentation medium at a volume ratio of 1:10, and ferment at 30°C for 16 hours, during which the reducing sugar concentration in the fermentation medium is controlled to be not less than 1.2% by adding reducing sugar solution, so as to obtain selenium-enriched yeast fermentation liquid;

[0076] The reducing sugar solution is composed of a glucose aqueous solution with a mass percentage concentration of 30% and sodium selenite in a weight ratio of 1:0.1, and the flow rate of the reducing sugar aqueous solution is 20 mg / h based on each liter of selenium-enriched yeast fermentation liquid;

[0077] The fermentation medium was prepared in Preparation Example 1;

[0078] C. Preparation of selenium-enriched yeast extract:

[0079] (1) First, the selenium-enriched yeast fermentation liquid prepared in B is heated to 70° C., autolyzed for 0.5 h, then the temperature is adjusted to 40° C., the pH is adjusted to 6.0, and then an extraction enzyme is added. The amount of the extraction enzyme added is calculated based on the weight of the selenium-enriched yeast fermentation liquid, which is 100 mg of extraction enzyme / g of selenium-enriched yeast fermentation liquid. The hydrolysis reaction is carried out for 12 h to obtain a hydrolyzate;

[0080] The extraction enzyme is transglutaminase;

[0081] (2) The hydrolyzate obtained in (1) is heated to 90° C., inactivated for 10 min, centrifuged at 3000 r / min for 15 min, the supernatant is removed, and then powdered and dried at 160° C. to obtain the selenium-enriched yeast extract.

[0082] Preparation Example 8

[0083] A selenium-enriched yeast extract, which is different from Preparation Example 7 in that the extraction method is as follows:

[0084] A. Yeast activation and expansion culture: After the beer yeast preserved in the glycerol tube is naturally thawed, 1.0 mL of the beer yeast is picked out and inoculated into a 500 mL conical flask containing 100 mL of activation culture medium at a volume ratio of 1:100, and cultured at a constant temperature of 30°C for 30 hours for activation. Then, the activated yeast is transferred to a culture tank containing 2 L of seed culture medium at a volume ratio of 1:20, and cultured at a constant temperature of 30°C for 36 hours for expansion to obtain an activated seed solution;

[0085] B. Selenium-enriched yeast fermentation culture: add the activated seed liquid into the sterilized 20L fermentation medium at a volume ratio of 1:10, and ferment at 30°C for 24 hours, during which the reducing sugar concentration in the fermentation medium is controlled to be not less than 1.2% by adding reducing sugar solution, so as to obtain selenium-enriched yeast fermentation liquid;

[0086] C. Preparation of selenium-enriched yeast extract:

[0087] (1) First, the selenium-enriched yeast fermentation liquid prepared in B is heated to 75° C., autolyzed for 0.75 h, then the temperature is adjusted to 45° C., the pH is adjusted to 6.5, and then an extraction enzyme is added. The amount of the extraction enzyme added is calculated based on the weight of the selenium-enriched yeast fermentation liquid, which is 130 mg of extraction enzyme / g of selenium-enriched yeast fermentation liquid. The hydrolysis reaction is carried out for 14 h to obtain a hydrolyzate;

[0088] (2) The hydrolyzate obtained in (1) was heated to 95° C. and inactivated for 12 min. The hydrolyzate was centrifuged at 3200 r / min for 18 min, the supernatant was removed, and then the hydrolyzate was spray-dried at 160° C. The other conditions were the same as those in Preparation Example 7, thereby obtaining the selenium-enriched yeast extract.

[0089] Preparation Example 9

[0090] A selenium-enriched yeast extract, which is different from Preparation Example 7 in that the extraction method is as follows:

[0091] A. Yeast activation and expansion culture: After the beer yeast preserved in the glycerol tube is naturally thawed, 1.0 mL of the beer yeast is picked out and inoculated into a 500 mL conical flask filled with 200 mL activation medium at a volume ratio of 1:200, and incubated at a constant temperature of 30°C for 36 hours for activation. Then, the activated yeast is transferred to a culture tank filled with 4 L seed culture medium at a volume ratio of 1:20, and incubated at a constant temperature of 30°C for 36 hours for expansion to obtain an activated seed solution;

[0092] B. Selenium-enriched yeast fermentation culture: add the activated seed liquid into 20L sterilized fermentation medium at a volume ratio of 1:10, and ferment at 35°C for 32 hours, during which the reducing sugar concentration in the fermentation medium is controlled to be not less than 1.2% by adding reducing sugar solution, so as to obtain selenium-enriched yeast fermentation liquid;

[0093] C. Preparation of selenium-enriched yeast extract:

[0094] (1) First, the selenium-enriched yeast fermentation liquid prepared in B is heated to 80° C., autolyzed for 1.0 h, then the temperature is adjusted to 50° C., the pH is adjusted to 7.0, and then an extraction enzyme is added. The amount of the extraction enzyme added is calculated based on the weight of the selenium-enriched yeast fermentation liquid, which is 160 mg of extraction enzyme / g of selenium-enriched yeast fermentation liquid. The hydrolysis reaction is carried out for 16 h to obtain a hydrolyzate;

[0095] (2) The hydrolyzate prepared in (1) was heated to 100°C, inactivated for 15 min, centrifuged at 3500 r / min for 20 min, the supernatant was removed, and then dried by spraying at 160°C. The other conditions were the same as those in Preparation Example 7, thereby obtaining the selenium-enriched yeast extract.

[0096] Preparation Example 10

[0097] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the seed culture medium used in A is composed of YPD culture medium and sodium selenite in a weight ratio of 1:0.001, other conditions are the same as Preparation Example 7.

[0098] Preparation Example 11

[0099] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the seed culture medium used in A is composed of YPD culture medium and sodium selenite in a weight ratio of 1:0.0015, other conditions are the same as Preparation Example 7.

[0100] Preparation Example 12

[0101] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the seed culture medium used in A is composed of YPD culture medium and sodium selenite in a weight ratio of 1:0.002, other conditions are the same as Preparation Example 7.

[0102] Preparation Example 13

[0103] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the seed culture medium used in A is composed of YPD culture medium and sodium selenite in a weight ratio of 1:0.003, other conditions are the same as Preparation Example 7.

[0104] Preparation Example 14

[0105] A selenium-enriched yeast extract, which is different from Preparation Example 7 in that, except that the reducing sugar solution in B is composed of a glucose aqueous solution with a mass percentage concentration of 40% and sodium selenite in a weight ratio of 1:0.2, and the flow rate of the reducing sugar aqueous solution is 30 mg / h per liter of selenium-enriched yeast fermentation liquid, other conditions are the same as Preparation Example 7.

[0106] Preparation Example 15

[0107] A selenium-enriched yeast extract, which is different from Preparation Example 7 in that, except that the reducing sugar solution in B is composed of a glucose aqueous solution with a mass percentage concentration of 50% and sodium selenite in a weight ratio of 1:0.25, and the flow rate of the reducing sugar aqueous solution is 55 mg / h per liter of selenium-enriched yeast fermentation liquid, other conditions are the same as Preparation Example 7.

[0108] Preparation Example 16

[0109] A selenium-enriched yeast extract, which is different from Preparation Example 7 in that, except that the reducing sugar solution in B is composed of a glucose aqueous solution with a mass percentage concentration of 60% and sodium selenite in a weight ratio of 1:0.3, and the flow rate of the reducing sugar aqueous solution is 80 mg / h per liter of selenium-enriched yeast fermentation liquid, other conditions are the same as Preparation Example 7.

[0110] Preparation Example 17

[0111] A selenium-enriched yeast extract, which is different from Preparation Example 7 in that, except that the reducing sugar solution in B is composed of a glucose aqueous solution with a mass percentage concentration of 70% and sodium selenite in a weight ratio of 1:0.4, and the flow rate of the reducing sugar aqueous solution is 90 mg / h per liter of selenium-enriched yeast fermentation liquid, other conditions are the same as Preparation Example 7.

[0112] Preparation Examples 18-22

[0113] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except for the use of the fermentation medium used in B, other conditions are the same as those in Preparation Example 7. The specific corresponding relationships are shown in the following table.

[0114] Group Fermentation medium Preparation Example 18 Prepared from Preparation Example 2 Preparation Example 19 Prepared from Preparation Example 3 Preparation Example 20 Prepared by Preparation Example 4 Preparation Example 21 Prepared from Preparation Example 5 Preparation Example 22 Prepared from Preparation Example 6

[0115] Preparation Example 23

[0116] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain in a weight ratio of 1:0.3:1, other conditions are the same as Preparation Example 7.

[0117] Preparation Example 24

[0118] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain in a weight ratio of 1:0.5:2, other conditions are the same as Preparation Example 7.

[0119] Preparation Example 25

[0120] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain in a weight ratio of 1:0.65:2.5, other conditions are the same as Preparation Example 7.

[0121] Preparation Example 26

[0122] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain in a weight ratio of 1:0.8:3, other conditions are the same as Preparation Example 7.

[0123] Preparation Example 27

[0124] A selenium-enriched yeast extract is different from Preparation Example 7 in that, except that the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain in a weight ratio of 1:1:4, other conditions are the same as Preparation Example 7.

[0125] Performance testing

[0126] The yeast fermented solid beverages containing the BAZ2B gene inhibitor prepared in each embodiment and comparative example were grouped for testing. Each group of test subjects consisted of 10 24-month-old C57 / BL6 nude mice. The spatial learning and memory ability of the elderly mice was tested by the Barnesmaze test. The specific testing steps and standards are as follows:

[0127] First, a yeast fermented solid beverage containing a BAZ2B gene inhibitor was mixed with warm water at a ratio of 1:1 to prepare a yeast fermented beverage containing a BAZ2B gene inhibitor. Then, the yeast fermented beverage containing a BAZ2B gene inhibitor was gavaged at 0.2 mL / mouse three times a day. After gavage for 30 days, the Barnesmaze experiment was started. The mice in the control group were not gavaged, and other living conditions of the mice were kept the same.

[0128] The elderly mice were trained to find the target hole for four consecutive days, with four training sessions a day and 15 minutes between each training session. On the fifth day, the mice's ability to find the target hole was tested to reflect their spatial learning and memory abilities. The time required for the mice to find the target hole for the first time and the number of explorations of the target hole were recorded, and the average values ​​were taken for recording. The behavioral experiment was double-blind and in compliance with the ethics of experimental animals.

[0129] Note: The Barnes maze test is a term for Alzheimer's disease announced by the National Committee for the Examination of Scientific and Technological Terminology in 2019. It can be used to detect the spatial reference memory and working memory abilities of animals. Therefore, it has reference value for whether it can delay aging, improve behavior and / or memory decline.

[0130] Example

[0131] Example 1

[0132] A solid beverage containing a human aging BAZ2B gene inhibitor, wherein the components and their corresponding weights are shown in Table 1, and the beverage is prepared by the following preparation method:

[0133] S1. First, mix the components in the raw material with deionized water in a weight percentage of 1:2, heat to 60°C and stir evenly, and circulate and homogenize 4 times at a pressure of 60MPa and a temperature of 65°C to obtain a mixed liquid;

[0134] The yeast extract in the raw material is prepared in Preparation Example 7;

[0135] S2: After sterilizing the mixed liquid prepared in S1, put it into the spray freeze dryer and control the air inlet temperature to 130℃ and the air inlet flow rate to 220m 3 / h, and the outlet air temperature is 90°C, so that a yeast fermented solid beverage containing BAZ2B gene inhibitor can be produced.

[0136] Embodiment 2-6

[0137] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that the components and their corresponding weights are shown in the following table.

[0138] Table: Components and corresponding weights in Examples 1-6 (kg)

[0139]

[0140]

[0141] Comparative Example 1

[0142] A yeast fermented solid beverage, which is different from Example 1 in that it does not contain BAZ2B gene inhibitor and yeast extract, and other conditions are the same as Example 1.

[0143] Comparative Example 2

[0144] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that it does not contain yeast extract, and other conditions are the same as Example 1.

[0145] The solid beverages prepared in the above Examples 1-6 and Comparative Examples 1-2 were sampled and subjected to performance testing. The time (s) for each group of mice to find the target hole for the first time and the number of explorations of the target hole (times) were measured respectively. The test results are recorded in the following table:

[0146]

[0147]

[0148] It can be seen from the above table that the solid beverages prepared in Examples 1-6, in addition to providing supplementary nutrients, all have excellent anti-aging effects, and can effectively improve the decline in behavioral ability and memory ability caused by aging. The elderly mice in the test group can find the target hole quickly, and the time it takes to find the target hole for the first time is 22.8-30.8s, and the behavioral ability is relatively high, and the number of explorations of the target hole is 3.3-3.7 times.

[0149] Furthermore, Example 3 and Example 4 are preferred embodiments, in which the time for finding the target hole for the first time is 22.8-30.8 s, and the number of explorations of the target hole is 3.3-3.7 times. It can be seen that when the BAZ2B gene inhibitor is used at a dosage of 0.04 kg, its effect of delaying aging and improving behavioral and memory decline is better.

[0150] When the dosage of the BAZ2B gene inhibitor increased to 0.06kg, its anti-aging and improvement effects did not continue to increase as expected, but there was no obvious change in the effect, or even a slight decrease. The reason may be that the dosage of the inhibitor was high and exceeded the upper limit of the mice's tolerance, which caused a certain stress response in the mice and increased the expression of the BAZ2B gene, which led to a weakening of the anti-aging and improvement of behavioral and memory decline effects.

[0151] In particular, the solid beverage prepared in Example 3 has the best effect in delaying aging, improving behavioral ability and memory decline. The time it takes to find the target hole for the first time is only 22.8 seconds, and the number of explorations of the target hole is as high as 3.7 times. The reason for this may be that, due to the components in the above-mentioned ratio, in addition to the BAZ2B gene inhibitor that can inhibit the BAZ2B gene, the selenium element, amino acids, B vitamins, nucleotides, polypeptides, etc. in the selenium-enriched yeast extract have a certain degree of synergistic effect on the inhibition of the BAZ2B gene inhibitor, and are beneficial to promoting the growth and development of new cells. Therefore, appropriately increasing the dosage of selenium-enriched yeast extract is beneficial to ensuring the efficacy of the solid beverage in delaying aging, improving behavioral ability and memory decline.

[0152] It can also be seen from the above table that, compared with Example 1 and the control group, Comparative Example 1 basically has no effect of delaying aging and improving behavioral and memory decline due to the lack of the addition of BAZ2B gene inhibitor and yeast extract. The time it takes to find the target hole for the first time is as high as 58.1s, which is 89% higher than that of Example 1; the number of explorations of the target hole is only 1.7 times, which is 48% lower than that of Example 1.

[0153] It can also be seen from the above table that, compared with Example 1, since yeast extract is not added for compounding, the efficacy of Comparative Example 2 in delaying aging and improving behavioral and memory decline is slightly reduced, and the time for finding the target hole for the first time is as high as 34.1s, which is 11% higher than that of Example 1; the number of explorations of the target hole is only 2.9 times, which is 12% lower than that of Example 1.

[0154] In summary, the BAZ2B gene inhibitor and yeast extract with the above components and proportions, after being added to solid beverages, can help delay human aging and have the effect of improving behavioral and memory decline. There is a certain compounding effect between the BAZ2B gene inhibitor and the yeast extract, which not only increases the nutritional value of the beverage, but also gives the beverage better health and anti-aging effects.

[0155] Embodiment 7-8

[0156] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that the yeast extract used is used differently, and the specific corresponding relationship is shown in the table below.

[0157] Group Yeast Extract Example 7 Prepared from Preparation Example 8 Example 8 Prepared from Preparation Example 9

[0158] The solid beverages prepared in the above Examples 7-8 were sampled and subjected to performance testing. The time (s) for each group of mice to find the target hole for the first time and the number of explorations of the target hole (times) were measured respectively. The test results are recorded in the following table:

[0159]

[0160] As can be seen from the above table, the solid beverages prepared in Example 1 and Examples 7-8, in addition to providing supplementary nutrients, have excellent anti-aging effects and can effectively improve the decline in behavioral ability and memory ability caused by aging. The elderly mice in the test group can find the target hole quickly, and the time it takes to find the target hole for the first time is 29.3-30.8s, and the behavioral ability is relatively high, and the number of explorations of the target hole is 3.3-3.4 times.

[0161] In particular, the solid beverage prepared in Example 7 has the best effect in delaying aging, improving behavioral ability and memory decline. The time it takes to find the target hole for the first time is only 29.3s, and its behavioral ability is relatively high. The number of explorations of the target hole is as high as 3.4 times. It can be seen that this process is the optimal process.

[0162] In summary, the yeast extract extracted by the above-mentioned extraction process has high contents of selenium, amino acids, B vitamins, nucleotides, polypeptides, etc., and has the best compounding effect on BAZ2B gene inhibitors. It can ensure the growth and development of new cells while enhancing the inhibitory expression of BAZ2B gene inhibitors.

[0163] Examples 9-12

[0164] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that the yeast extract used is used differently, and the specific corresponding relationship is shown in the table below.

[0165] Group Yeast Extract Example 9 Prepared from Preparation Example 10 Example 10 Prepared from Preparation Example 11 Embodiment 11 Prepared from Preparation Example 12 Example 12 Prepared from Preparation Example 13

[0166] The solid beverages prepared in the above Examples 9-12 were sampled and subjected to performance testing. The time (s) for each group of mice to find the target hole for the first time and the number of explorations of the target hole (times) were measured respectively. The test results are recorded in the following table:

[0167]

[0168] As can be seen from the above table, the solid beverages prepared in Example 1 and Examples 9-12 not only provide supplementary nutrients, but also have excellent anti-aging effects, and can effectively improve the decline in behavioral ability and memory ability caused by aging. The elderly mice in the test group can find the target hole quickly, and the time for them to find the target hole for the first time is 26.3-30.8s, and their behavioral ability is relatively high, and the number of explorations of the target hole is 3.3-3.5 times.

[0169] Furthermore, the solid beverages prepared in Examples 9-11 have good effects on delaying aging, improving behavioral ability and memory decline, and the time to find the target hole for the first time is 26.3-27.7s, and the number of explorations of the target hole is 3.4-3.5 times. It can be seen that when the seed culture medium used in A is composed of YPD culture medium and sodium selenite in a weight ratio of 1: (0.001-0.002), the extraction effect of the yeast extract is better, and the retention of effective ingredients such as selenium, amino acids, and nucleic acids is higher.

[0170] In particular, the solid beverage prepared in Example 10 has the best effect of delaying aging, improving behavioral ability and memory decline, and the time for finding the target hole for the first time is only 26.3s, and the number of explorations of the target hole is as high as 3.5 times. It can be seen that when the seed culture medium used in A is composed of YPD culture medium and sodium selenite at a weight ratio of 1:0.0015, the extraction effect of the yeast extract is the best, the retention of active ingredients such as selenium, amino acids, nucleic acids, etc. is the highest, and it has the strongest synergistic effect on the inhibition of BAZ2B gene inhibitors.

[0171] In summary, the seed culture medium with the above components and proportions can increase the selenium content in yeast cells through primary screening without affecting the normal growth and development of yeast, thereby ensuring the retention of each effective ingredient in the yeast extract and enhancing its combined inhibitory effect with the BAZ2B gene inhibitor.

[0172] Examples 13-16

[0173] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that the yeast extract used is used differently, and the specific corresponding relationship is shown in the table below.

[0174] Group Yeast Extract Example 13 Prepared from Preparation Example 14 Embodiment 14 Prepared from Preparation Example 15 Embodiment 15 Prepared from Preparation Example 16 Example 16 Prepared from Preparation Example 17

[0175] The solid beverages prepared in the above Examples 9-12 were sampled and subjected to performance testing. The time (s) for each group of mice to find the target hole for the first time and the number of explorations of the target hole (times) were measured respectively. The test results are recorded in the following table:

[0176]

[0177]

[0178] As can be seen from the above table, the solid beverages prepared in Example 1 and Examples 13-16 not only provide supplementary nutrients, but also have excellent anti-aging effects, and can effectively improve the decline in behavioral ability and memory ability caused by aging. The elderly mice in the test group can find the target hole quickly, and the time it takes to find the target hole for the first time is 25.8-30.8s, and the behavioral ability is relatively high, and the number of explorations of the target hole is 3.3-3.5 times.

[0179] Furthermore, the solid beverages prepared in Examples 13-15 have good effects on delaying aging, improving behavioral ability and memory decline, and the time to find the target hole for the first time is 25.8-27.2s, and the number of explorations of the target hole is 3.5 times. It can be seen that the reducing sugar solution in B is composed of a 50% glucose aqueous solution and sodium selenite in a weight ratio of 1:0.25, and the flow rate of the reducing sugar aqueous solution is 55 mg / h based on the selenium-enriched yeast fermentation liquid per liter. The retention of active ingredients such as selenium, amino acids, and nucleic acids in the yeast extract is relatively high, and it has a better compounding effect on the BAZ2B gene inhibitor.

[0180] In particular, the solid beverage prepared in Example 15 has the best effect of delaying aging, improving behavioral ability and memory decline. The time for finding the target hole for the first time is only 25.8s, and the number of explorations of the target hole is as high as 3.5 times. It can be seen that the reducing sugar solution in B is composed of a 60% glucose aqueous solution and sodium selenite in a weight ratio of 1:0.3. The flow rate of the reducing sugar aqueous solution is 80 mg / h per liter of selenium-enriched yeast fermentation liquid. This process is the optimal process.

[0181] In summary, the reducing sugar solution with the above components and proportions can not only ensure the nutritional supply for the normal proliferation and growth of yeast during the feeding process, but also has a certain screening effect, which is beneficial to the enrichment and proliferation of selenium-enriched yeast, and the sodium selenite with the above concentration and feeding amount is not easy to affect the growth and development of selenium-enriched yeast, thereby giving the solid beverage better anti-aging and health-care effects.

[0182] Examples 17-21

[0183] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that the yeast extract used is used differently, and the specific corresponding relationship is shown in the table below.

[0184] Table: Comparison of yeast extract usage in Examples 17-21

[0185]

[0186]

[0187] The solid beverages prepared in the above Examples 17-21 were sampled and subjected to performance testing. The time (s) for each group of mice to find the target hole for the first time and the number of explorations of the target hole (times) were measured respectively. The test results are recorded in the following table:

[0188]

[0189] As can be seen from the above table, the solid beverages prepared in Example 1 and Examples 17-21, in addition to providing supplementary nutrients, have excellent anti-aging effects and can effectively improve the decline in behavioral ability and memory ability caused by aging. The elderly mice in the test group can find the target hole quickly, and the time it takes to find the target hole for the first time is 30.1-30.8s, and the behavioral ability is relatively high, and the number of explorations of the target hole is 3.2-3.4 times.

[0190] In particular, the solid beverage prepared in Example 19 has the best effect of delaying aging, improving behavioral ability and memory decline. The time for finding the target hole for the first time is only 30.1s, and the number of explorations of the target hole is as high as 3.4 times. It can be seen that the fermentation medium under this component and ratio is conducive to the growth and extraction of selenium-enriched yeast; when the amount of sodium selenite in the fermentation medium exceeds 0.015kg, it delays aging, improves behavioral ability and memory decline. The reason for this may be that the initial concentration of sodium selenite is high, which is not conducive to the growth of yeast species.

[0191] In summary, the fermentation medium with the above components and proportions can not only ensure the nutrient supply for the normal proliferation and growth of yeast during the fermentation process, but also has a screening effect, which is beneficial to the enrichment and proliferation of selenium-enriched yeast. When the sodium selenite content is high, it is not conducive to the initial growth and development of yeast.

[0192] Examples 22-26

[0193] A solid beverage containing a human aging BAZ2B gene inhibitor, which is different from Example 1 in that the yeast extract used is used differently, and the specific corresponding relationship is shown in the table below.

[0194] Group Yeast Extract Embodiment 22 Prepared from Preparation Example 23 Embodiment 23 Prepared from Preparation Example 24 Embodiment 24 Prepared from Preparation Example 25 Embodiment 25 Prepared from Preparation Example 26 Embodiment 26 Prepared from Preparation Example 27

[0195] The solid beverages prepared in the above Examples 22-26 were sampled and subjected to performance testing. The time (s) for each group of mice to find the target hole for the first time and the number of explorations of the target hole (times) were measured respectively. The test results are recorded in the following table:

[0196]

[0197] As can be seen from the above table, the solid beverages prepared in Examples 22-26 not only provide supplementary nutrients, but also have excellent anti-aging effects, and can effectively improve the decline in behavioral ability and memory ability caused by aging. The elderly mice in the test group can find the target hole quickly, and the time for finding the target hole for the first time is 26.6-28.4s, which is 8-14% lower than that in Example 1; and the behavioral ability is relatively high, and the number of explorations of the target hole is 3.4-3.6 times, which is 3-9% higher than that in Example 1.

[0198] Furthermore, the solid beverages prepared in Examples 23-25 ​​have good effects on delaying aging, improving behavioral ability and memory loss, and the time for finding the target hole for the first time is 26.6-27.2s, and the number of explorations of the target hole is 3.5-3.6 times. It can be seen that when the extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain, and the weight ratio is 1: (0.5-0.8): (2-3), the extraction efficiency of yeast is high, and the retention of effective ingredients such as selenium, amino acids, and nucleic acids in the obtained yeast extract is high, thus giving it a better compounding effect on BAZ2B gene inhibitors, ensuring the efficacy of the solid beverage in delaying aging, improving behavioral ability and memory loss.

[0199] In particular, the solid beverage prepared in Example 24 has the best effect in delaying aging, improving behavioral ability and memory decline. The time it takes to find the target hole for the first time is only 26.6 seconds, and the number of explorations of the target hole is as high as 3.6 times. It can be seen that the extraction enzyme ratio is the optimal ratio.

[0200] In summary, the extraction enzymes with the above components and ratios have better extraction effects, and the obtained yeast extract has the highest retention of various nutrients and effective ingredients, which is beneficial to improving the compounding effect of yeast extract on BAZ2B gene inhibitors.

[0201] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A selenium-enriched yeast extract capable of delaying aging, characterized in that: The selenium content in the selenium-enriched yeast extract is not less than 2000 mg / kg; The extraction method of the selenium-enriched yeast extract is as follows: A. Yeast activation and expansion culture: First, pick beer yeast and inoculate it into the activation culture medium at a volume ratio of 1: (100-200) and culture it at a constant temperature of 30℃ for 24-36 hours for activation. Then transfer the activated yeast to the seed culture medium for expansion culture to obtain the activated seed liquid; The activation medium used in the A is YPD medium; The seed culture medium used in A is composed of YPD culture medium and sodium selenite, and the weight ratio of YPD culture medium to sodium selenite is 1: (0.001-0.002); B. Selenium-enriched yeast fermentation culture: Add the activated seed liquid into the sterilized fermentation medium, and ferment at 30-35°C for 16-32 hours, during which the reducing sugar concentration in the fermentation medium is controlled to be not less than 1.2%, to obtain selenium-enriched yeast fermentation liquid; The fermentation medium contains sodium selenite; C. Preparation of selenium-enriched yeast extract: (1) First, the selenium-enriched yeast fermentation liquid prepared in B is heated to 70-80°C, and autolyzed for 0.5-1h, and then the temperature is adjusted to 40-50°C and the pH is adjusted to 6.0-7.0, and then an extraction enzyme is added. The amount of the extraction enzyme added is calculated based on the weight of the selenium-enriched yeast fermentation liquid, and the amount is 100-160 mg of extraction enzyme / g of selenium-enriched yeast fermentation liquid. The hydrolysis reaction is carried out for 12-16h to obtain a hydrolyzate; The extracting enzyme in C is composed of glutamine aminotransferase, nuclease and papain, and the weight ratio is 1: (0.5-0.8): (2-3); The hydrolyzate prepared in (1) is heated to 90-100°C, inactivated for 10-15 minutes, and then centrifuged at 3000-3500 r / min for 15-20 minutes, the supernatant is taken, and then spray-dried to obtain the selenium-enriched yeast extract.

2. The selenium-enriched yeast extract according to claim 1, characterized in that In B, the reducing sugar solution was added in a flow manner to control the reducing sugar concentration in the fermentation medium to be no less than 1.2%; The reducing sugar solution is composed of a glucose aqueous solution with a mass percentage concentration of 40-60% and sodium selenite in a weight ratio of 1: (0.2-0.3). The flow rate of the reducing sugar aqueous solution is 30-80 mg / h based on each liter of selenium-enriched yeast fermentation liquid.

3. The selenium-enriched yeast extract according to claim 1, characterized in that The fermentation medium described in B, calculated by weight percentage, has the following raw material composition and content: Yeast powder 3.5-4.5% Soybean peptone 3.2-3.6% Glucose 1.6-2.0% Trehalose 1.0-1.6% Adenine 0.005-0.01% Sodium selenite 0.015-0.020% The balance is water.

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