An automated biological detection system and method

Abstract

The present invention relates to the field of biological detection technology, and discloses an automated biological detection system and method. The automated biological detection system includes: a sample tray, on which a reagent tank and a cleaning tank are fixedly arranged at a radial position; a reagent tray, on which a sample tank is arranged at a radial position; a rotation driving structure, connected to the reagent tray and the sample tray; an aspiration and injection member, which is used for aspirating and injecting samples; a first lifting driving member, connected to the aspiration and injection member; a first position sensor, installed at the aspiration and injection station; a detection member, which is used for detecting samples; a second lifting driving member, connected to the detection member; a second position sensor, installed at the detection station; and a controller, electrically connected to the rotation driving member, the first lifting driving member, the electromagnetic control valve, the second lifting driving member, the detection member, the first position sensor, and the second position sensor. By performing aspiration, injection, and cleaning at the same station, sequential operations can be completed.

Description

Technical Field

[0001] The present invention relates to the technical field of biological detection, and particularly relates to an automated biological detection system and method. Background Art

[0002] With the development and progress of science and technology as well as instruments, humans are exploring more and more frequently. The analysis of biomolecular interactions is of great significance for revealing the molecular mechanisms of life processes. Since there is a huge number of biomolecules, high-throughput analysis tools are required to systematically analyze the interactions between biomolecules.

[0003] Currently, for the detection of biomolecules, most are manual operations. Manual detection has certain detection errors, and the operation is cumbersome, which is not convenient for mass production detection. Summary of the Invention

[0004] To solve the above technical problems, the present invention provides an automated biological detection system, and the automated biological detection system includes:

[0005] A sample tray, in which a reagent groove and a cleaning groove are fixedly arranged at a radial position. The inside of the reagent groove is used for containing reagents or placing open bottles containing reagents; the inside of the cleaning groove is used for placing cleaning liquids or open bottles containing cleaning liquids;

[0006] A reagent tray, in which a sample groove is arranged at a radial position. The inside of the sample groove is used for containing samples or placing open bottles containing samples;

[0007] A rotation driving structure, connected to the reagent tray and the sample tray, and used for driving the sample tray and the reagent tray to rotate;

[0008] An inhalation and injection member, used for inhaling and injecting samples;

[0009] A first lifting driving member, connected to the inhalation and injection member, and used for driving the inhalation and injection member to lift. The inhalation and injection member is installed at an inhalation and injection station;

[0010] A first position sensor, installed at the inhalation and injection station, and used for sensing the entry of the sample groove, the reagent groove, and the cleaning groove into the inhalation and injection station;

[0011] A detection member, used for detecting samples.

[0012] A second lifting driving member, connected to the detection member, and used for driving the detection member to lift. The detection member is installed at a detection station;

[0013] A second position sensor, installed at the detection station, and used for sensing the entry of the reagent groove and the cleaning groove into the detection station;

[0014] A controller is used to control a rotation driving member to drive a reagent disc to rotate, so that a sample trough enters an inhalation and injection station; a first lifting driving member drives an inhalation and injection member into the interior of the sample trough, and the lifting amount of the inhalation and injection member corresponds to a preset inhalation amount; the controller controls the reagent disc to rotate so that the sample trough leaves the inhalation and injection station; the sample disc rotates so that a reagent trough enters the inhalation and injection station, and the first lifting driving member drives the inhalation and injection member into the interior of the reagent trough and injects a sample into the interior of the reagent trough; the sample disc rotates so that the reagent trough enters a detection station, and a second lifting driving member drives a detection member into the interior of the reagent trough for detection; a cleaning trough enters the inhalation and injection station, and the first lifting driving member drives the inhalation and injection member into the interior of the cleaning trough for cleaning; the cleaning trough enters the detection station, and the detection member enters the interior of the cleaning trough for cleaning.

[0015] Preferably, a cleaning member is arranged inside the cleaning trough.

[0016] Preferably, the cleaning member is one or a combination of a stirring blade, a bubble generator or an ultrasonic generator.

[0017] Preferably, the rotation driving structure includes a motor, a two-way one-way pulley, a second driven pulley and a first driven pulley; the output shaft of the motor is coaxially connected with the two-way one-way pulley, the first driven pulley is coaxially and fixedly connected with the reagent disc, the second driven pulley is coaxially and fixedly connected with the sample disc, the two-way one-way pulley is connected with the first driven pulley and the second driven pulley by a belt, the transmission directions of the two-way one-way pulley are opposite, when the motor rotates forward, it drives the first driven pulley to rotate to drive the reagent disc to rotate; when the motor rotates reversely, it drives the second driven pulley to rotate to drive the sample disc to rotate.

[0018] Preferably, the inhalation and injection member includes an electromagnetic control valve, a guide rod and a containing barrel. The guide rod is fixedly connected to the end of the first lifting driving member. One end of the guide rod slides through a blocking piece and extends into the interior of the containing barrel. The containing barrel is fixedly connected with the blocking piece. The blocking piece is connected to the first lifting driving member by a spring. A piston is fixedly connected to the end of the guide rod, and the piston is hermetically and slidably embedded into the interior of the containing barrel.

[0019] Preferably, the interior of the guide rod and the piston is a hollow structure and is connected to a connection port. The connection port is connected to an inert gas source through a pipeline. An electromagnetic control valve is arranged in the connection port. The electromagnetic control valve is used to control the flow rate of the inert gas. When the inhalation and injection member finishes injection, the electromagnetic control valve opens, and the inert gas enters the interior of the containing barrel. The inert gas enters the interior of the reagent trough through the containing barrel, avoiding the reagent entering the interior of the containing barrel to form residues, and the inert gas entering the interior of the reagent liquid promotes the mixing of the sample and the reagent.

[0020] Preferably, a one-way blocking piece is arranged inside the piston, and the one-way blocking piece is used to limit the internal flow direction of the hollow structure.

[0021] Preferably, a needle is detachably and sealingly connected to the port of the storage barrel.

[0022] Preferably, the automated biological detection system further includes a housing, a sample disk and a reagent disk are rotatably arranged inside the housing, and one ends of the sample disk and the reagent disk extend out of the housing.

[0023] The present invention also provides an automated biological detection method, which applies the above-mentioned automated biological detection system. The automated biological detection system includes the following steps:

[0024] S1. The rotation driving member drives the reagent disk to rotate so that the sample groove enters the suction and injection station;

[0025] S2. The first lifting driving member drives the suction and injection member to enter the inside of the sample groove for suction, and the lifting amount of the suction and injection member corresponds to a preset suction amount;

[0026] S3. The reagent disk rotates to make the sample groove leave the suction and injection station, and the sample disk rotates to make the reagent groove enter the suction and injection station;

[0027] S4. The first lifting driving member drives the suction and injection member to enter the inside of the reagent groove and injects the sample into the inside of the reagent groove;

[0028] S5. The sample disk rotates to make the reagent groove enter the detection station;

[0029] S6. The second lifting driving member drives the detection member to enter the inside of the reagent groove for detection;

[0030] S7. The cleaning groove enters the suction and injection station, and the suction and injection member enters the inside of the cleaning groove for cleaning;

[0031] S8. The cleaning groove enters the detection station, and the detection member enters the inside of the cleaning groove for cleaning.

[0032] The technical effects and advantages of the present invention: By performing suction, injection, and cleaning at the same station, sequential operations can be completed, simplifying the device structure and enabling sequential operations. The integrated operations of suction, injection, and cleaning are completed, with a high degree of automation. Description of the Drawings

[0033] Figure 1 It is a three-dimensional structural schematic diagram of an automated biological detection system proposed by the present invention.

[0034] Figure 2 It is an internal structural schematic diagram of an automated biological detection system proposed by the present invention.

[0035] Figure 3 This is a schematic diagram of the internal combined structure of an automated biological detection system proposed by the present invention.

[0036] Figure 4 This is a three-dimensional structure diagram of an inhalation and injection component in an automated biological detection system proposed by the present invention.

[0037] Figure 5 This is a schematic diagram of the internal structure of an inhalation and injection component in an automated biological detection system proposed by the present invention.

[0038] Figure 6 This is a schematic diagram of a detection component in an automated biological detection system proposed by the present invention.

[0039] Figure 7 This is a flowchart of an automated biological detection method proposed by the present invention.

[0040] Explanation of reference numerals: housing 1, controller 2, sample tray 3, reagent tray 4, first lifting drive member 5, inhalation and injection member 6, sample trough 7, first driven pulley 8, motor 9, double-sided one-way pulley 10, second driven pulley 11, reagent trough 12, cleaning trough 13, second lifting drive member 14, detection member 15, connection port 16, electromagnetic control valve 17, spring 18, guide rod 19, blocking piece 20, storage barrel 21, needle 22, piston 23, one-way blocking piece 24. Detailed implementation manners

[0041] The present invention will be further described in detail below with reference to the accompanying drawings and specific implementation manners. The embodiments of the present invention are given for purposes of illustration and description, and are not exhaustive or limit the present invention to the disclosed form. Many modifications and variations are obvious to those of ordinary skill in the art. The embodiments are selected and described to better illustrate the principles and practical applications of the present invention, and enable those of ordinary skill in the art to understand the present invention and thus design various embodiments with various modifications suitable for specific purposes.

[0042] Embodiment 1

[0043] Reference Figures 1 to 3 , in this embodiment, an automated biological detection system is proposed. The automated biological detection system includes: sample tray 3, reagent tray 4, inhalation and injection member 6, detection member 15, and controller 2.

[0044] The housing 1 forms an installation support structure. The housing 1 can be a box structure. Support legs are provided at the bottom of the housing 1, and a backing plate is fixedly connected to the bottom of the support legs. The support legs support the housing 1 to a specified height, and the setting of the backing plate increases the stability of the support of the support legs. Universal wheels are provided at the four corners of the bottom of the housing 1. The setting of the universal wheels facilitates the movement of the housing 1, and a clamping plate for braking is provided on the universal wheels to facilitate the fixation of the housing 1.

[0045] The sample tray 3 is rotatably connected to the housing 1. A reagent tank 12 and a cleaning tank 13 are fixedly provided at the radial position of the sample tray 3. The inside of the reagent tank 12 is used to hold reagents or for placing open bottles containing reagents. The inside of the cleaning tank 13 is used to place cleaning liquid or for placing open bottles containing cleaning liquid. One end of the sample tray 3 can extend out of the housing 1, so as to facilitate the placement and replacement of samples and cleaning liquid.

[0046] The reagent tray 4 is rotatably connected to the housing 1. A sample slot 7 is provided at the radial position of the reagent tray 4. The inside of the sample slot 7 is used to hold samples or for placing open bottles containing samples. Of course, the sample tray 3 and the reagent tray 4 can be open, which will not be elaborated here.

[0047] The rotation drive structure is connected to the reagent tray 4 and the sample tray 3, and is used to drive the sample tray 3 and the reagent tray 4 to rotate. The rotation drive structure includes a motor 9, a double-sided one-way pulley 10, a second driven pulley 11 and a first driven pulley 8. The motor 9 is fixedly installed on the housing 1, and the output shaft of the motor 9 is coaxially connected with the double-sided one-way pulley 10. The first driven pulley 8 is coaxially and fixedly connected to the reagent tray 4, and the second driven pulley 11 is coaxially and fixedly connected to the sample tray 3. The double-sided one-way pulley 10 is connected to the first driven pulley 8 and the second driven pulley 11 by a belt. The transmission directions of the double-sided one-way pulley 10 are opposite. When the motor 9 rotates forward, it drives the first driven pulley 8 to rotate, causing the reagent tray 4 to rotate. At this time, the second driven pulley 11 cannot rotate. When the motor 9 rotates in the reverse direction, it drives the second driven pulley 11 to rotate, causing the sample tray 3 to rotate, thereby controlling the conversion of workstations.

[0048] The suction and injection member 6 is arranged in the housing 1 in a liftable manner and is used for sucking and injecting samples.

[0049] The first lifting drive member 5 is installed inside the housing 1 and is connected to the suction and injection member 6, and is used to drive the suction and injection member 6 to lift. The suction and injection member 6 is installed at the suction and injection workstations.

[0050] Reference Figure 4 、 Figure 5, the suction and injection member 6 may include an electromagnetic control valve 17, a guide rod 19, a storage barrel 21 and a guide rod 19. The guide rod 19 may be fixedly connected to the end of the first lifting drive member 5. One end of the guide rod 19 slides through the blocking piece 20 and extends into the interior of the storage barrel 21. The storage barrel 21 is fixedly connected to the blocking piece 20. The blocking piece 20 is connected to the first lifting drive member 5 by a spring 18. A piston 23 is fixedly connected to the end of the guide rod 19. The piston 23 is hermetically and slidably embedded into the interior of the storage barrel 21. When the first lifting drive member 5 drives the suction and injection member 6 to descend, the storage barrel 21 extends into the interior of the sample tank 7. The blocking piece 20 covers the top of the sample tank 7 to compress the spring 18. The guide rod 19 pushes the piston 23 into the storage barrel 21. When the first lifting drive member 5 drives the suction and injection member 6 to lift, under the action of the spring 18, the blocking piece 20 resets, causing the piston 23 to slide in the reverse direction to form a negative pressure inside the storage barrel 21, thereby completing the suction of the sample. The storage barrel 21 enters the interior of the reagent tank 12, causing the piston 23 to slide inside the storage barrel 21 to complete the injection of the sample. The interior of the guide rod 19 and the piston 23 is a hollow structure and is connected to the connection port 16. The connection port 16 is connected to an inert gas source through a pipeline. An electromagnetic control valve 17 is provided in the connection port 16. The electromagnetic control valve 17 is used to control the flow rate of the inert gas. When the suction and injection member 6 completes the injection, the electromagnetic control valve 17 opens, and the inert gas enters the interior of the storage barrel 21. The inert gas enters the interior of the reagent tank 12 through the storage barrel 21, avoiding the entry of the reagent into the interior of the storage barrel 21 to form residues. The inert gas enters the interior of the reagent liquid to promote the mixing of the sample and the reagent. And the inert gas protects the mixed sample reagent. By controlling the telescopic amount of the first lifting drive member 5, the sliding amount of the piston 23 inside the storage barrel 21 is adjusted, thereby controlling the injection amount of the sample piece. A one-way blocking piece 24 is provided inside the piston 23. The one-way blocking piece 24 is used to limit the flow direction inside the hollow structure, avoiding the entry of the reagent into the interior of the hollow structure, and ensuring the entry of the inert gas into the interior of the storage barrel 21. A needle 22 is detachably and hermetically connected to the port of the storage barrel 21. The setting of the needle 22 facilitates the suction and injection of the suction and injection member 6 into the interior of the sample tank 7 and the reagent tank 12, avoiding the contamination caused by the entry of the storage barrel 21 into the reagent. Replacing the needle 22 avoids cross-contamination.

[0051] The first position sensor is installed at the suction and injection station for sensing the entry of the sample tank 7, the reagent tank 12, and the cleaning tank 13 into the suction and injection station.

[0052] The detection member 15 is liftably arranged inside the housing 1 and is used for detecting the sample.

[0053] The second lifting drive member 14 is installed inside the housing 1 and is connected to the detection member 15 for driving the detection member 15 to lift and lower. The detection member 15 is installed at the detection station.

[0054] The second position sensor is installed at the detection station and is used to sense the entry of the reagent tank 12 and the cleaning tank 13 into the detection station.

[0055] The controller 2 is electrically connected to the rotation driving member, the first lifting driving member 5, the electromagnetic control valve 17, the second lifting driving member 14, the detection member 15, the first position sensor, and the second position sensor. Put the sample into the interior of the sample tank 7, and put the corresponding detection reagent into the interior of the reagent tank 12. The controller 2 controls the rotation driving member to drive the reagent disc 4 to rotate, so that the sample tank 7 enters the suction and injection station. The first lifting driving member 5 drives the suction and injection member 6 to descend into the interior of the sample tank 7. The lifting amount of the suction and injection member 6 corresponds to a preset suction amount. The controller 2 controls the rotation of the reagent disc 4 to make the sample tank 7 leave the suction and injection station. The sample disc 3 rotates to make the reagent tank 12 enter the suction and injection station. The first lifting driving member 5 drives the suction and injection member 6 to descend into the interior of the reagent tank 12 and injects the sample into the interior of the reagent tank 12. The sample disc 3 rotates to make the reagent tank 12 enter the detection station. The second lifting driving member 14 drives the detection member 15 to enter the interior of the reagent tank 12 for detection. At this time, the cleaning tank 13 enters the suction and injection station. The first lifting driving member 5 drives the suction and injection member 6 to enter the interior of the cleaning tank 13 for cleaning. Thus, the cleanliness of the suction and injection member 6 is ensured. The cleaning tank 13 enters the detection station, and the detection member 15 enters the interior of the cleaning tank 13 for cleaning. By performing suction, injection, and cleaning at the same station, the structure of the device is simplified, and sequential operations can be achieved. The integrated operation of suction, injection, and cleaning is completed. A cleaning member is provided inside the cleaning tank 13. The cleaning effect of the cleaning liquid can be enhanced through the cleaning member. The cleaning member can be one or a combination of a stirring blade, a bubble generator, or an ultrasonic generator. The controller 2 can include a display member and an input member. The suction and injection amount and the detection method can be input through the input member, so that data can be input. The display member can display the detection signal for easy observation.

[0056] Embodiment 2

[0057] In this embodiment, an automated biological detection method is proposed, including the following steps:

[0058] S1. The rotation driving member drives the reagent disc 4 to rotate, so that the sample tank 7 enters the suction and injection station.

[0059] S2. The first lifting driving member 5 drives the suction and injection member 6 to enter the interior of the sample tank 7 for suction. The lifting amount of the suction and injection member 6 corresponds to a preset suction amount.

[0060] S3. The reagent disc 4 rotates to make the sample tank 7 leave the suction and injection station. The sample disc 3 rotates to make the reagent tank 12 enter the suction and injection station.

[0061] S4. The first lifting drive 5 drives the suction and injection member 6 into the interior of the reagent tank 12 and injects the sample into the interior of the reagent tank 12.

[0062] S5. The sample tray 3 rotates to move the reagent tank 12 into the detection station.

[0063] S6. The second lifting drive 14 drives the detection member 15 into the interior of the reagent tank 12 for detection.

[0064] S7. The cleaning tank 13 moves to the suction and injection station, and the suction and injection member 6 enters the interior of the cleaning tank 13 for cleaning.

[0065] S8. The cleaning tank 13 moves to the detection station, and the detection member 15 enters the interior of the cleaning tank 13 for cleaning.

[0066] Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art and related fields without creative efforts shall fall within the protection scope of the present invention. The structures, devices, and operation methods not specifically described and explained in the present invention, unless otherwise specified and limited, are implemented according to the conventional means in the art.

Claims

1. An automated biological detection system, characterized in that: the automated biological detection system includes: a sample tray, on which reagent grooves and cleaning grooves are fixedly arranged at radial positions. The inside of the reagent groove is used for containing reagents or placing open bottles containing reagents; the inside of the cleaning groove is used for placing cleaning liquid or open bottles containing cleaning liquid; a reagent tray, on which a sample groove is arranged at a radial position. The inside of the sample groove is used for containing samples or placing open bottles containing samples; a rotation driving structure, connected to the reagent tray and the sample tray, and used for driving the reagent tray and the sample tray to rotate; the rotation driving structure includes a motor, a two-way one-way pulley, a second driven pulley and a first driven pulley; the output shaft of the motor is coaxially connected with the two-way one-way pulley, the first driven pulley is coaxially and fixedly connected with the reagent tray, the second driven pulley is coaxially and fixedly connected with the sample tray, the two-way one-way pulley is connected with the first driven pulley and the second driven pulley through a belt, and the transmission directions of the two-way one-way pulley are opposite. When the motor rotates forward, it drives the first driven pulley to rotate to make the reagent tray rotate; when the motor rotates backward, it drives the second driven pulley to rotate to make the sample tray rotate; an inhalation and injection member, used for inhaling and injecting samples; the inhalation and injection member includes an electromagnetic control valve, a guide rod and a containing barrel. The guide rod is fixedly connected to the end of a first lifting driving member. One end of the guide rod slides through a blocking piece and extends into the inside of the containing barrel. The containing barrel is fixedly connected with the blocking piece. The blocking piece is connected to the first lifting driving member through a spring. A piston is fixedly connected to the end of the guide rod, and the piston is hermetically and slidably embedded into the inside of the containing barrel; the inside of the guide rod and the piston is a hollow structure and is connected to a connection port. The connection port is connected to an inert gas source through a pipeline; an electromagnetic control valve is arranged in the connection port, and the electromagnetic control valve is used for controlling the flow rate of the inert gas. When the inhalation and injection member finishes injection, the electromagnetic control valve opens, and the inert gas enters the inside of the containing barrel. The inert gas enters the inside of the reagent groove through the containing barrel, avoiding the entry of reagents into the inside of the containing barrel to form residues, and the inert gas entering the inside of the reagent liquid promotes the mixing of the sample and the reagent; a first lifting driving member, connected to the inhalation and injection member, and used for driving the inhalation and injection member to lift. The inhalation and injection member is installed at the inhalation and injection station; a first position sensor, installed at the inhalation and injection station, and used for sensing the entry of the sample groove, the reagent groove and the cleaning groove into the inhalation and injection station; a detection member, used for detecting samples; a second lifting driving member, connected to the detection member, and used for driving the detection member to lift. The detection member is installed at the detection station; a second position sensor, installed at the detection station, and used for sensing the entry of the reagent groove and the cleaning groove into the detection station; A controller is used to control a rotation driving member to drive a reagent disk to rotate, so that a sample tank enters an inhalation and injection station; a first lifting driving member drives an inhalation and injection member into the interior of the sample tank, and the lifting amount of the inhalation and injection member corresponds to a preset inhalation amount; the controller controls the reagent disk to rotate so that the sample tank leaves the inhalation and injection station; the sample disk rotates to make the reagent tank enter the inhalation and injection station, and the first lifting driving member drives the inhalation and injection member into the interior of the reagent tank and injects the sample into the interior of the reagent tank; the sample disk rotates to make the reagent tank enter the detection station, and a second lifting driving member drives a detection member into the interior of the reagent tank for detection; the cleaning tank enters the inhalation and injection station, and the first lifting driving member drives the inhalation and injection member into the interior of the cleaning tank for cleaning; the cleaning tank enters the detection station, and the detection member enters the interior of the cleaning tank for cleaning.

2. An automated biological detection system according to claim 1, wherein: A cleaning member is provided inside the cleaning tank.

3. An automated biological detection system according to claim 2, wherein: The cleaning member is one or a combination of a stirring blade, a bubble generator, or an ultrasonic generator.

4. An automated biological detection system according to claim 1, wherein: A one-way blocking piece is provided inside the piston, and the one-way blocking piece is used to restrict the flow direction inside the hollow structure.

5. An automated biological detection system according to claim 1, wherein: A needle is detachably and sealingly connected to the port of the storage barrel.

6. An automated biological detection system according to claim 1, wherein: The automated biological detection system further includes a housing, the sample disk and the reagent disk are rotatably arranged inside the housing, and one end of the sample disk and the reagent disk extends out of the housing.

7. An automated biological detection method using the automated biological detection system according to any one of claims 1-6, wherein: The automated biological detection system includes the following steps: S1. The rotation driving member drives the reagent disk to rotate, so that the sample tank enters the inhalation and injection station; S2. The first lifting driving member drives the inhalation and injection member into the interior of the sample tank for inhalation, and the lifting amount of the inhalation and injection member corresponds to a preset inhalation amount; S3. The reagent disk rotates to make the sample tank leave the inhalation and injection station, and the sample disk rotates to make the reagent tank enter the inhalation and injection station; S4. The first lifting driving member drives the inhalation and injection member into the interior of the reagent tank and injects the sample into the interior of the reagent tank; S5. The sample disk rotates to make the reagent tank enter the detection station; S6. The second lifting driving member drives the detection member into the interior of the reagent tank for detection; S7. The cleaning tank enters the inhalation and injection station, and the inhalation and injection member enters the interior of the cleaning tank for cleaning; S8. The cleaning tank enters the detection station, and the detection member enters the interior of the cleaning tank for cleaning.

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