A non-human primate behavioral data acquisition device and data acquisition method

Abstract

The present invention discloses a non-human primate behavioral data acquisition device and a data acquisition method, wherein the non-human primate behavioral data acquisition device includes an animal movement device, a plurality of stereo cameras and an analysis terminal, the animal movement device includes a treadmill, a hanger, an animal restraint and a plurality of fluorescent spray paints, the hanger is set above the running belt of the treadmill, the hanger is provided with a suspension rod extending toward the running belt, the end of the suspension rod is provided with a first buckle, the animal restraint is provided with a second buckle, and the first buckle is correspondingly buckled with the first buckle; the plurality of fluorescent spray paints are used to mark the joints; the number of the plurality of stereo cameras is not less than four, and the plurality of stereo cameras are arranged at intervals in the peripheral area of ​​the treadmill to collect animal gait data and transmit the data to the analysis terminal. The present invention can effectively collect non-human primate behavioral gait data and analyze the non-human primate behavioral gait data.

Description

Technical Field

[0001] The present invention relates to the technical field of non-human primate behavioral data collection, and in particular to a non-human primate behavioral data collection device and a data collection method. Background Art

[0002] In medical experiments targeting a specific disease, collecting research data through experimental animals is an extremely important part, such as research on acute spinal cord injury. For acute spinal cord injury, monkeys are the most suitable experimental animals. At least three types of data need to be collected for monkeys on acute spinal cord injury. The first type is the behavioral data of normal individuals, the second type is the behavioral data of individuals with corresponding injuries, and the third type is the behavioral data of injured individuals in the recovery state after treatment. By comparing the three types of behavioral data, a clear study and exploration of acute spinal cord injury can be conducted. However, at present, there is a lack of a device that can accurately collect the walking trajectory of non-human primates for the collection of the three types of behavioral data. Summary of the invention

[0003] The purpose of the present invention is to provide a non-human primate behavioral data acquisition device and data acquisition method. By using monkeys as experimental animals of non-human primates, the monkey gait data can be effectively collected, the error of traditional behavioral acquisition methods can be greatly reduced, and the monkey gait data can be analyzed to form the joint movement trajectory of the monkey's forelimbs and hindlimbs.

[0004] To achieve the above object, the present invention provides the following technical solutions:

[0005] A non-human primate behavioral data acquisition device includes an animal movement device, a plurality of stereo cameras and an analysis terminal. The animal movement device includes a treadmill, a hanger, an animal restraint suit and a plurality of fluorescent spray paints. The hanger is mounted above the running belt of the treadmill, the hanger is provided with a suspension rod extending toward the running belt, the end of the suspension rod is provided with a first buckle, the animal restraint suit is worn on the non-human primate, and the animal restraint suit is provided with a second buckle for the first buckle to be correspondingly buckled with the first buckle to restrict the non-human primate on the running belt of the treadmill; the plurality of fluorescent spray paints are used to mark multiple joints of the non-human primate; the number of the plurality of stereo cameras is not less than four, and the plurality of stereo cameras are arranged at intervals in the peripheral area of ​​the treadmill to collect animal gait data of the non-human primate on the running belt and transmit the data to the analysis terminal; the analysis terminal processes the collected gait data and transforms it into the movement trajectory of the front and rear limb joints of the non-human primate.

[0006] As a further technical solution of the present invention: the hanger includes a main frame, which is erected above the treadmill parallel or vertically relative to the running belt of the treadmill, and the main frame includes two relatively arranged support frames, a mounting rod and a hanger seat, and the two support frames are relatively arranged at the front and rear ends of the treadmill or on both sides of the treadmill; the two ends of the mounting rod are relatively arranged on the two support frames, so that the mounting rod is installed between the two support frames; the hanger seat is provided with a movable end and a mounting end, the movable end is arranged on the mounting rod, and the hanger is uprightly arranged on the mounting end.

[0007] As a further technical solution of the present invention: the hanger includes a forelimb hanger, which is arranged at the rear end of the treadmill, and the forelimb hanger includes a seat frame and a hanging rod, the seat frame is arranged at the rear end of the treadmill, one end of the hanging rod is arranged on the seat frame, and the other end of the hanging rod is extended toward the running belt, and the other end of the hanging rod is extended to both sides relative to each other to provide two forelimb hanging plates, the forelimb hanging plates are located behind the hanger, so that the armpits of the non-human primate can clamp the forelimb hanging plates, so that the forelimbs of the non-human primate are hung behind the forelimb hanging plates, thereby fixing the forelimbs of the non-human primate.

[0008] As a further technical solution of the present invention: there are two types of animal restraint garments, namely a vest type used before surgery and a restraint garment type used after surgery, the vest type includes a first vest body, the restraint garment type includes a second vest body with the same structure as the first vest type and a restraint abdominal belt integrally connected to the second vest body.

[0009] As a further technical solution of the present invention: a front chest section is provided in the middle part of the first vest body, and two rear belt sections are oppositely provided on both sides of the vest body, a sleeve section is provided between each rear belt section and the front chest section, and a sleeve opening is provided on the sleeve section, and a first connecting structure is provided between the two rear belt sections for detachable connection, and buckles are respectively provided on the outer surfaces of the two rear belt sections so that the front chest section can fit the front chest of the non-human primate, and the arms pass through the sleeve opening, and the two rear belt sections overlap at the back and are connected by the connecting structure, and the second buckle is passed through the buckles of the two rear belt sections, and the first vest body is worn on the non-human primate.

[0010] As a further technical solution of the present invention: the restraint abdominal belt is provided with a main body section and two connecting sections arranged on both sides of the main body section, the top of the main body section is connected to the bottom ends of the front chest section and the cuff section of the second vest body, and a second connecting structure is provided between the two connecting sections for detachable connection, so that after the second vest body is worn on the chest position of the non-human primate, the main body section fits the front abdomen and the muscular lateral abdomen, and the two connecting sections are overlapped and connected on the monkey's back through the second connecting structure, and the restraint abdominal belt is worn on the abdomen of the non-human primate to cover and protect the sutured wounds on the non-human primate.

[0011] In addition, the present invention necessarily provides a data collection method for the non-human primate behavioral data collection device.

[0012] A data collection method for a non-human primate behavioral data collection device is as follows: non-human primate behavioral gait analysis includes behavioral gait training, behavioral gait data collection and data analysis; before starting behavioral gait data collection, the experimental animal needs to receive behavioral gait training so that the experimental animal can maintain cooperation during the formal data collection process; the training is conducted three times a week until the animal fully cooperates, and the animal with good performance will be appropriately rewarded during the training process; after the training is completed, behavioral gait data collection can be started, and the time for collecting the behavioral gait data of the experimental animal is before surgery, 3 months after surgery, and 1 year after surgery;

[0013] Behavioral gait data collection: The collection process of behavioral gait data needs to be completed by at least four stereo cameras in conjunction with an analysis terminal equipped with behavioral gait analysis-related software MotionRecorder. Before data collection, the experimental animals are marked with multiple joints on the body by fluorescent spray paint, including bilateral iliac spines, hip joints, knee joints, ankle joints, toe joints, shoulder joints, elbow joints, and wrist joints. Then, the experimental animals walk on the animal motion device of the behavioral gait analyzer at a speed of 3 km / h. The animal gait data is collected by four stereo cameras with a camera frequency of 60 Hz. The collected gait data is transmitted to the analysis terminal, filtered and processed by the 3DCalculator software in the analysis terminal, and the real-time 3D coordinates of each fluorescent marker are obtained. The collected three-dimensional model data is converted into a stick-shaped three-dimensional model. Finally, 10 consecutive walking cycles are completed to carry out the next step of behavioral gait data analysis.

[0014] Behavioral gait data analysis: The data analysis process of behavioral gait needs to be completed through the behavioral gait analysis related software KineAnalyzer in the analysis terminal. First, the behavioral gait data converted into a stick-shaped three-dimensional model is imported into the KineAnalyzer software to complete the gait setting, obtain the stride length of the front and rear limbs, and the proportion of the limb support period. Finally, the fluorescent marker points of each joint collected by the data are averaged to obtain the joint movement trajectory of the front and rear limbs.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: the present invention proposes a data acquisition method for a non-human primate behavioral data acquisition device, which effectively acquires the behavioral gait data of non-human primates through analysis among an animal motion device, a plurality of stereo cameras and an analysis terminal, greatly reduces the error of traditional behavioral acquisition methods, and analyzes the behavioral gait data of non-human primates to form the joint movement trajectory of the forelimbs and hindlimbs. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 Layout diagram of the non-human primate behavioral data collection device.

[0017] Figure 2 A first schematic diagram of an animal locomotion device.

[0018] Figure 3 The second schematic diagram of the animal movement device.

[0019] Figure 4 This is a diagram of the unfolded state of the vest-type animal restraint suit.

[0020] Figure 5 This is a diagram of the wearing state of the vest-type animal restraint clothing.

[0021] Figure 6 This is a diagram of the unfolded state of the restraint-type animal restraint suit.

[0022] Figure 7 This is a diagram of the wearing state of a restraint-type animal restraint suit.

[0023] Figure 8 Schematic diagram of the rod-shaped three-dimensional model of the analysis terminal.

[0024] Fig. 9 This is the movement trajectory convergence diagram of the control group.

[0025] Fig.10 This is the convergence diagram of the motion trajectories of the experimental group.

[0026] Fig.11 This is a comparison chart of the effective time of collection between the control group and the experimental group. DETAILED DESCRIPTION

[0027] The specific implementation of the present invention is described in detail below in conjunction with the accompanying drawings. It should be understood that the specific implementation described here is only used to illustrate and explain the present invention, and is not used to limit the protection scope of the present invention.

[0028] See also Figure 1 , Figure 2 and Figure 3A non-human primate behavior data collection device includes an animal movement device 10, a plurality of stereo cameras 20 and an analysis terminal 30, wherein the animal movement device 10 includes a treadmill 11, a hanger 12, an animal restraint suit 13 and a plurality of fluorescent spray paints 14, wherein the hanger 12 is mounted above the running belt of the treadmill 11, and the hanger 12 is provided with a suspension rod 101 extending toward the running belt, and the end of the suspension rod 101 is provided with a first buckle 102, and the animal restraint suit 13 is worn on the monkey, and the animal restraint suit 13 is provided with a second buckle 103 for the first buckle 102 to be connected with the first buckle 103. 02 correspondingly buckled to restrict the monkey on the running belt of the treadmill 11; a number of fluorescent spray paints 14 are used to mark multiple joints of the monkey's body, marking the monkey's bilateral iliac spines, hip joints, knee joints, ankle joints, toe joints, shoulder joints, elbow joints and wrist joints in turn; the number of a number of stereo cameras 20 is not less than four, and the number of stereo cameras 20 is arranged at intervals in the peripheral area of ​​the treadmill 11 to collect animal gait data of the monkey on the running belt and transmit the data to the analysis terminal 30; the analysis terminal 30 processes the collected gait data and transforms it into the movement trajectory of the monkey's front and rear limb joints.

[0029] Furthermore, the hanger 12 includes a main frame 121, which is erected above the treadmill 11 parallel to or vertically relative to the running belt of the treadmill 11. The main frame 121 includes two relatively arranged support frames 1211, a mounting rod 1212 and a hanger seat 1213. The two support frames 1211 are relatively arranged at the front and rear ends of the treadmill 11 or on both sides of the treadmill 11; the two ends of the mounting rod 1212 are relatively arranged on the two support frames 1211, so that the mounting rod 1212 is installed between the two support frames 1211; the hanger seat 1213 is provided with a movable end and a mounting end, the movable end is arranged on the mounting rod 1212, and the hanger 101 is uprightly arranged on the mounting end.

[0030] Furthermore, the movable end can be movably disposed on the mounting rod 1212 along the mounting rod 1212 , and a first locking structure is disposed on the movable end, and the movable end is fixed to the mounting rod 1212 through the first locking structure.

[0031] Furthermore, the suspension rod 101 is movably arranged upright on the mounting end, and a second locking structure is provided on the mounting end, and the suspension rod 101 is fixed to the mounting end by the second locking structure.

[0032] Further, the hanger 12 includes a forelimb hanger 122, which is arranged at the rear end of the treadmill 11, and includes a seat frame 1221 and a hanging rod 1222, wherein the seat frame 1221 is arranged at the rear end of the treadmill 11, one end of the hanging rod 1222 is arranged on the seat frame 1221, and the other end of the hanging rod 1222 is extended toward the running belt, and the other end of the hanging rod 1222 is relatively extended to both sides and provided with two forelimb hanging plates 1223, wherein the forelimb hanging plates 1223 are located behind the hanger 101, so that the monkey's armpits can clamp the forelimb hanging plates 1223, so that the monkey's forelimbs are hung behind the forelimb hanging plates 1223, thereby fixing the monkey's forelimbs. It can be understood that the forelimb hanger 122 is mainly used to accurately collect the movement trajectory of the monkey's hind limb joints and increase the data comparison items.

[0033] For further information, see Figure 4 , Figure 5 , Figure 6 and Figure 7 There are two types of animal restraint garments 13, namely a vest type 131 used before surgery and a restraint garment type 132 used after surgery. The vest type 131 includes a first vest body 41, and the restraint garment type 132 includes a second vest body 42 with the same structure as the first vest type 131 and a restraint abdominal belt 43 integrally connected to the second vest body 42.

[0034] Furthermore, a front chest section 411 is provided in the middle of the first vest body 41, and two rear belt sections 412 are oppositely provided on both sides of the vest body, a sleeve section 413 is provided between each rear belt section 412 and the front chest section 411, and a sleeve opening 414 is provided on the sleeve section 413, and a first connecting structure 4121 is provided between the two rear belt sections 412 for detachable connection, and buckles 415 are respectively provided on the outer surfaces of the two rear belt sections 412, so that the front chest section 411 can fit the monkey's chest, and the monkey's arms can pass through the sleeve opening 414, and the two rear belt sections 412 are overlapped on the monkey's back and connected by the connecting structure, and the second buckle 103 is passed through the buckles 415 of the two rear belt sections 412, and the first vest body 41 is put on the monkey.

[0035] Furthermore, the restraining abdominal belt 43 is provided with a main body section 431 and two connecting sections 432 arranged on both sides of the main body section 431. The top of the main body section 431 is connected to the bottom ends of the front chest section 411 and the sleeve section 413 of the second vest body 42. A second connecting structure 4321 is provided between the two connecting sections 432 for detachable connection. After the second vest body 42 is worn on the monkey's chest, the main body section 431 fits the monkey's front abdomen and muscular flanks, and the two connecting sections 432 are overlapped and connected on the monkey's back through the second connecting structure 4321. The restraining abdominal belt 43 is worn on the monkey's abdomen to cover and protect the sutured wounds on the monkey's body.

[0036] Furthermore, the first connection structure 4121 and the second connection structure 4321 may be formed by a zipper, a Velcro or a buckle structure. In this embodiment, the first connection structure 4121 and the second connection structure 4321 are formed by a Velcro.

[0037] Understandable, combined with reference Figure 8 The present invention provides a data collection method for a non-human primate behavioral data collection device, as follows: non-human primate behavioral gait analysis includes behavioral gait training, behavioral gait data collection and data analysis; before starting behavioral gait data collection, the experimental animals need to receive behavioral gait training so that the experimental animals can maintain cooperation during the formal data collection process; the training is conducted three times a week until the animals fully cooperate, and animals with good performance will be appropriately rewarded during the training process; after the training is completed, behavioral gait data collection can be started, and the time for collecting behavioral gait data of the experimental animals is before surgery, 3 months after surgery, and 1 year after surgery;

[0038] Behavioral gait data collection: The behavioral gait data collection process needs to be completed by at least four stereo cameras 20 in conjunction with an analysis terminal 30 equipped with behavioral gait analysis related software MotionRecorder software. Before data collection, the experimental animals are marked with multiple joints on the body by fluorescent spray paint 14, including bilateral iliac spines, hip joints, knee joints, ankle joints, toe joints, shoulder joints, elbow joints, and wrist joints; then, the experimental animals walk on the animal motion device 10 of the behavioral gait analyzer at a speed of 3km / h, and the animal gait data is collected by four stereo cameras with a camera frequency of 60Hz. The collected gait data is transmitted to the analysis terminal 30, filtered and processed by the 3DCalculator software in the analysis terminal 30, and the real-time 3D coordinates of each fluorescent marker are obtained, and the collected three-dimensional model data is converted into a stick-shaped three-dimensional model. Finally, 10 consecutive walking cycles are completed to carry out the next step of behavioral gait data analysis;

[0039] Behavioral gait data analysis: The data analysis process of behavioral gait needs to be completed through the behavioral gait analysis related software KineAnalyzer software in the analysis terminal 30. First, the behavioral gait data converted into a stick-shaped three-dimensional model is imported into the KineAnalyzer software to complete the gait setting, and the stride length of the front and rear limbs and the proportion of the limb support period are obtained. Finally, the fluorescent marking points of each joint collected by the data are averaged to obtain the joint movement trajectory of the front and rear limbs.

[0040] Specifically, the patent of the present invention is compared with the original comparative method and an experimental scheme is designed to prove the advantages of the patent of the present invention.

[0041] Number of animals: 20 cynomolgus monkeys were divided into 2 groups. The behavioral data of the experimental group were collected by the patented method of the present invention, and the data of the control group were collected by the original behavioral method. The behavioral data of each animal was collected for 100 seconds, and the effective data time of the standard gait of each cynomolgus monkey's behavioral data was recorded.

[0042] Duration of animal behavior data collection: Each behavioral collection lasts 100 seconds, with a total of 10 animals. Each animal repeats the test 3 times and takes the average value. The collection process of behavioral gait data needs to be completed by at least four stereo cameras in conjunction with an analysis terminal equipped with behavioral gait analysis related software MotionRecorder software. Before data collection, the experimental animals are marked with multiple joints on the body by fluorescent spray paint, including bilateral iliac spines, hip joints, knee joints, ankle joints, toe joints, shoulder joints, elbow joints, and wrist joints; then, the experimental animals walk on the animal motion device of the behavioral gait analyzer at a speed of 3km / h, and the animal gait data is collected by four stereo cameras with a camera frequency of 60Hz. The collected gait data is transmitted to the analysis terminal, filtered and automatically identified by the 3DCalculator software in the analysis terminal, and the real-time 3D coordinates of each fluorescent marker are obtained, and the collected three-dimensional model data is converted into a stick-shaped three-dimensional model. Among them, the gait data that can be automatically identified by the analysis terminal is the valid data of the standard gait, which is counted in seconds.

[0043] See also Fig. 9 , Fig.10 and Fig.11 Animal behavior data were collected from the experimental and control groups of crab-eating monkeys. The data collection duration of the experimental group was 20.2±2.7 seconds, and the data collection duration of the control group was 11.8±3.7 seconds.

[0044] from Fig. 9 , Fig.10 From the comparison of trajectories, we can see that the trajectory of the control group is messy and irregular, and most of the data are invalid, while the gait trajectory of the experimental group is clear and regular, and most of the data are valid.

[0045] In summary, the data acquisition method of the non-human primate behavioral data acquisition device of the present invention effectively collects the behavioral gait data of non-human primates through analysis between the animal movement device 10, a plurality of stereo cameras 20 and the analysis terminal 30, greatly reduces the error of the traditional behavioral acquisition method, and analyzes the behavioral gait data of non-human primates to form the joint movement trajectory of the front and rear limbs.

[0046] As long as it does not violate the creative ideas of the present invention, any combination of the various embodiments of the present invention shall be regarded as the contents disclosed by the present invention; within the technical concept of the present invention, any combination of various simple modifications of the technical scheme and different embodiments that does not violate the creative ideas of the present invention shall be within the protection scope of the present invention.

Claims

1. A non-human primate behavioral data acquisition device, characterized in that: The invention comprises an animal exercise device (10), a plurality of stereo cameras (20) and an analysis terminal (30). The animal exercise device (10) comprises a treadmill (11), a hanger (12), an animal restraint suit (13) and a plurality of fluorescent spray paints (14). The hanger (12) is mounted above the running belt of the treadmill (11). The hanger (12) is provided with a suspension rod (101) extending toward the running belt. The end of the suspension rod (101) is provided with a first buckle (102). The animal restraint suit (13) is worn on a non-human primate. The animal restraint suit (13) is provided with a second buckle (103) for the second buckle (103) to be used for the second buckle (103). A buckle (102) is correspondingly buckled with the first buckle (102) to restrict the non-human primate on the running belt of the treadmill (11); a plurality of fluorescent spray paints (14) are used to mark multiple joints of the non-human primate; a number of stereo cameras (20) is not less than four, and the plurality of stereo cameras (20) are arranged at intervals in the peripheral area of ​​the treadmill (11) to collect animal gait data of the non-human primate on the running belt and transmit the data to the analysis terminal (30); the analysis terminal (30) processes the collected gait data and converts it into a movement trajectory of the front and rear limb joints of the non-human primate; The hanger (12) comprises a main frame (121), the main frame (121) being mounted above the treadmill (11) in parallel or vertical relation to the running belt of the treadmill (11), the main frame (121) comprising two supporting frames (1211) arranged opposite to each other, a mounting rod (1212) and a hanging rod seat (1213), the two supporting frames (1211) being arranged opposite to each other at the front and rear ends of the treadmill (11) or at both sides of the treadmill (11); the two ends of the mounting rod (1212) being arranged opposite to each other on the two supporting frames (1211), so that the mounting rod (1212) is mounted between the two supporting frames (1211); the hanging rod seat (1213) is provided with a movable end and a mounting end, the movable end being arranged on the mounting rod (1212), and the hanging rod (101) being arranged upright on the mounting end; The hanger (12) comprises a forelimb hanger (122), the forelimb hanger (122) being arranged at the rear end of the treadmill (11), the forelimb hanger (122) comprising a seat frame (1221) and a hanging rod (1222), the seat frame (1221) being arranged at the rear end of the treadmill (11), one end of the hanging rod (1222) being arranged on the seat frame (1221), the other end of the hanging rod (1222) being arranged to extend toward the running belt, the other end of the hanging rod (1222) being arranged to extend to opposite sides and to have two forelimb hanging plates (1223), the forelimb hanging plates (1223) being arranged at the rear of the hanger (101), so that the armpits of the non-human primate can clamp the forelimb hanging plates (1223), so that the forelimbs of the non-human primate are hung at the rear of the forelimb hanging plates (1223), thereby fixing the forelimbs of the non-human primate.

2. The non-human primate behavioral data acquisition device according to claim 1, characterized in that: There are two types of animal restraint garments (13), namely a vest type (131) used before surgery and a restraint garment type (132) used after surgery. The vest type (131) includes a first vest body (41), and the restraint garment type (132) includes a second vest body (42) having the same structure as the first vest type (131) and a restraint abdominal belt (43) integrally connected to the second vest body (42).

3. The non-human primate behavioral data acquisition device according to claim 2, characterized in that: A front chest section (411) is provided in the middle of the first vest body (41), and two rear belt sections (412) are provided on opposite sides of the vest body. A sleeve section (413) is provided between each rear belt section (412) and the front chest section (411), and a sleeve opening (414) is provided on the sleeve section (413). A first connecting structure (4121) is provided between the two rear belt sections (412) for detachable connection. Buckles (415) are provided on the outer surfaces of the two rear belt sections (412) so that the front chest section (411) can fit the front chest of a non-human primate and the arms can pass through the sleeve opening (414). The two rear belt sections (412) are overlapped at the back and connected through the connecting structure. The second buckle (103) is passed through the buckles (415) of the two rear belt sections (412), and the first vest body (41) is worn on the non-human primate.

4. The non-human primate behavioral data acquisition device according to claim 3, characterized in that: The restraining abdominal belt (43) is provided with a main body section (431) and two connecting sections (432) arranged on both sides of the main body section (431). The top of the main body section (431) is connected to the front chest section (411) and the bottom end of the sleeve section (413) of the second vest body (42). A second connecting structure (4321) is provided between the two connecting sections (432) for detachable connection. After the second vest body (42) is worn on the chest of a non-human primate, the main body section (431) fits the front abdomen and the lateral abdomen. The two connecting sections (432) are overlapped and connected on the monkey's back through the second connecting structure (4321). The restraining abdominal belt (43) is worn on the abdomen of the non-human primate to cover and protect the sutured wound on the non-human primate.

5. A data collection method for the non-human primate behavioral data collection device according to any one of claims 1 to 4, characterized in that: Behavioral gait analysis of non-human primates includes behavioral gait training, behavioral gait data collection and data analysis; Before the start of behavioral gait data collection, experimental animals need to receive behavioral gait training so that they can maintain cooperation during the formal data collection process; the training is conducted three times a week until the animals fully cooperate, and animals with good performance will be appropriately rewarded during the training process; after the training is completed, behavioral gait data collection can begin. The time for collecting behavioral gait data of experimental animals is before surgery, 3 months after surgery, and 1 year after surgery; Behavioral gait data collection: The behavioral gait data collection process needs to be completed by at least four stereo cameras (20) in conjunction with an analysis terminal (30) equipped with behavioral gait analysis related software MotionRecorder software. Before data collection, the experimental animals are marked with multiple joints on the body using fluorescent paint (14), including bilateral iliac spines, hip joints, knee joints, ankle joints, toe joints, shoulder joints, elbow joints, and wrist joints. Then, the experimental animals walk on the animal movement device (10) of the behavioral gait analyzer at a speed of 3 km / h. The animal gait data is collected by four stereo cameras with a camera frequency of 60 Hz. The collected gait data is transmitted to the analysis terminal (30), filtered and processed by the 3DCalculator software in the analysis terminal (30), and the real-time 3D coordinates of each fluorescent marker are obtained, and the collected three-dimensional model data is converted into a stick-shaped three-dimensional model. Finally, 10 consecutive walking cycles are completed to carry out the next step of behavioral gait data analysis. Behavioral gait data analysis: The behavioral gait data analysis process needs to be completed through the behavioral gait analysis related software KineAnalyzer software in the analysis terminal (30). First, the behavioral gait data converted into a rod-shaped three-dimensional model is imported into the KineAnalyzer software to complete the gait setting and obtain the stride length of the front and rear limbs and the proportion of the limb support period. Finally, the data collected from each joint fluorescent marker point is averaged to obtain the joint movement trajectory of the front and rear limbs.

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