A multifunctional liquid reaction device

Abstract

The present invention discloses a multifunctional liquid reaction device, which includes a pathway switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the liquid storage unit; the pathway switching unit, the injection unit and the reaction unit are respectively communicably connected to the control unit. The multifunctional liquid reaction device provided in the present application can provide an accurate, automatic and efficient experimental device for chemical detection experiments. It can realize functions such as accurate liquid inlet, multiple liquids participating in the reaction, constant temperature heating (time, temperature controllable, settable), reaction and automatic processing of experimental results. It can realize accurate quantitative automatic injection and automatic cleaning of multiple reagents. The use of in-situ constant temperature heating technology and PID constant temperature heating technology ensures more accurate temperature control and improves efficiency and accuracy. It is worthy of large-scale promotion and use.

Description

Technical Field

[0001] The invention relates to the technical field of experimental instruments, in particular to a multifunctional liquid reaction device. Background Art

[0002] In biochemistry teaching or scientific research, when conducting a certain biochemical experiment, the experimenter often needs to add two or more reagents and mix them, or let them stand, or heat them to a constant temperature, and keep them for a certain period of time, and finally get the experimental results according to a certain phenomenon or concentration. In this process, the experimenter usually uses manual methods, such as measuring cylinders, measuring bottles, droppers, etc. to add and mix liquids. This operation method causes many human interference factors. When adding reagents manually, errors often occur randomly due to the experimenter's mood, technique, proficiency, operating habits, etc., and are uncontrollable. Therefore, different results or even large errors occur under the same experimental conditions.

[0003] At the same time, when doing biochemical experiments, a large number of repeated experiments are often required for the same experimental conditions. A large amount of repetitive work such as adding reagents, constant temperature digestion, and result judgment also requires manual participation, which adds a lot of workload for the experimenters.

[0004] In addition, in the digestion process of biochemical experiments, reagents are usually loaded into test tubes and digested with the help of specific digestion instruments. Some experiments require higher digestion temperatures, or some reagents are highly corrosive and toxic. Therefore, it is dangerous to move the test tubes and easily cause injuries. Summary of the invention

[0005] The present invention provides a multifunctional liquid reaction device, which can realize functions such as accurate liquid feeding, multiple liquids participating in the reaction, constant temperature heating (time and temperature are controllable and settable), automatic reaction, automatic processing of experimental results, etc.

[0006] The present invention provides the following scheme:

[0007] A multifunctional liquid reaction device, comprising:

[0008] A passage switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the liquid storage unit; the passage switching unit, the injection unit and the reaction unit are respectively connected to the control unit in a communicable manner;

[0009] The passage switching unit includes a common pipeline, a first branch pipeline and a plurality of second branch pipelines; the liquid storage unit is connected to the common pipeline, the reaction unit is connected to the first branch pipeline; the second branch pipelines are respectively used to be connected to different solution containers in a one-to-one correspondence;

[0010] The control unit is used to perform the following operations:

[0011] Generate an experimental strategy according to the received experimental requirements, wherein the experimental strategy includes aspiration strategy, injection strategy and reaction strategy;

[0012] The liquid aspiration strategy is sent to the pathway switching unit and the injection unit respectively, the pathway switching unit is used to connect the common pipeline with the target second branch pipeline according to the liquid aspiration strategy, and the target second branch pipeline is connected to the container of the target solution; the injection unit is used to transfer the target solution to the liquid storage unit after the common pipeline is connected with the target second branch pipeline;

[0013] The injection strategy is sent to the pathway switching unit and the injection unit respectively, the pathway switching unit is used to connect the common pipeline with the first branch pipeline according to the injection strategy, and the injection unit is used to transfer the target solution from the liquid storage unit to the reaction unit after the common pipeline is connected with the first branch pipeline;

[0014] The reaction strategy is sent to the reaction unit so that the reaction unit forms a target reaction environment inside the reaction unit according to the reaction strategy.

[0015] Preferably: the reaction unit includes an inlet end and an outlet end, and the inlet end and the outlet end are respectively configured with a pressure relief valve; the pressure relief valve is communicatively connected to the control unit; the control unit is used to send the reaction strategy to the pressure relief valve, so that the pressure relief valve adjusts the on-off state according to the reaction strategy.

[0016] Preferably: the reaction strategy includes an injection start instruction and an injection end instruction; the pressure relief valve is used to adjust to a conducting state after receiving the injection start instruction and to adjust to a blocking state after receiving the injection end instruction.

[0017] Preferably: the reaction unit includes a main body made of transparent material, on which a photoelectric signal acquisition component is arranged, the photoelectric signal acquisition component is communicatively connected to the control unit, and the control unit is used to judge the concentration of the reagent in the main body according to the situation of the reagent absorbing the target wavelength light collected by the photoelectric signal acquisition component.

[0018] Preferably: the photoelectric signal acquisition component includes a light source and a photocell arranged relatively on the main body, and the reaction unit also includes a light-shielding shell arranged outside the main body.

[0019] Preferably: the reaction unit comprises a temperature control component, the temperature control component is communicatively connected to the control unit, and the temperature control component is used to adjust the temperature inside the reaction unit according to the reaction strategy so as to form a target reaction environment inside the reaction unit.

[0020] Preferably: the channel switching unit comprises a multi-channel switching valve island including a switching power component, and the multi-channel switching valve island comprises a sapphire valve core and a valve head having a polytrifluoroethylene corrosion-resistant layer.

[0021] Preferably: the valve hole of the multi-channel switching valve island is positioned by means of a code disk optical coupling, and the switching power component includes a planetary reduction box motor.

[0022] Preferably: the injection unit comprises a vertical injection pump; the vertical injection pump is respectively connected to the liquid storage unit and a pure water supply unit including a pure water valve; the liquid storage unit comprises a liquid storage ring.

[0023] Preferably: the control unit includes an STM32F373CBT6 single chip microcomputer, and the control unit is also connected to an audible and visual alarm component.

[0024] According to the specific embodiments provided by the present invention, the present invention discloses the following technical effects:

[0025] Through the present invention, a multifunctional liquid reaction device can be realized. In one implementation mode, the device may include a pathway switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the liquid storage unit; the pathway switching unit, the injection unit and the reaction unit are respectively communicably connected to the control unit; the pathway switching unit includes a common pipeline, a first branch pipeline and a plurality of second branch pipelines; the liquid storage unit is connected to the common pipeline, and the reaction unit is connected to the first branch pipeline; the second branch pipelines are respectively used to be connected to different solution containers in a one-to-one correspondence. The multifunctional liquid reaction device provided in the present application can provide an accurate, automatic and efficient experimental device for chemical detection experiments. It can realize functions such as accurate liquid inlet, multiple liquids participating in the reaction, constant temperature heating (time, temperature controllable, settable), reaction and automatic processing of experimental results. It can realize accurate quantitative automatic injection and automatic cleaning of multiple reagents. The use of in-situ constant temperature heating technology and PID constant temperature heating technology ensures more accurate temperature control and improves efficiency and accuracy. It is worth promoting and using on a large scale.

[0026] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0028] Figure 1 is a schematic structural diagram of a multifunctional liquid reaction device provided by an embodiment of the present invention;

[0029] Figure 2 is a circuit diagram of the first part of a multifunctional liquid reaction device provided by an embodiment of the present invention;

[0030] Figure 3 is a circuit diagram of the second part of a multifunctional liquid reaction device provided by an embodiment of the present invention;

[0031] Figure 4 is a circuit diagram of the third part of a multifunctional liquid reaction device provided by an embodiment of the present invention;

[0032] Figure 5 is a circuit diagram of the fourth part of a multifunctional liquid reaction device provided by an embodiment of the present invention;

[0033] Figure 6 The present invention provides a flowchart of a water quality permanganate index experiment using the reaction device.

[0034] In the figure: a channel switching unit 1, a common pipeline 11, a first branch pipeline 12, a second branch pipeline 13, an injection unit 2, a liquid storage unit 3, a reaction unit 4, a control unit 5, an audio-visual alarm component 51, a solution container 6, a pressure relief valve 7, a light source 81, a photocell 82, a temperature control component 9, and a pure water valve 10. DETAILED DESCRIPTION

[0035] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field belong to the scope of protection of the present invention.

[0036] Example

[0037] See also Figure 1 , is a multifunctional liquid reaction device provided by an embodiment of the present invention, such as Figure 1 As shown, the device may include:

[0038] A passage switching unit 1, an injection unit 2, a liquid storage unit 3, a reaction unit 4 and a control unit 5; the injection unit 2 is connected to the liquid storage unit 3; the passage switching unit 1, the injection unit 2 and the reaction unit 4 are respectively connected to the control unit 5 in a communicable manner;

[0039] The passage switching unit 1 includes a common pipeline 11, a first branch pipeline 12 and a plurality of second branch pipelines 13; the liquid storage unit 3 is connected to the common pipeline 11, the reaction unit 4 is connected to the first branch pipeline 12; the second branch pipelines 13 are respectively used to be connected to different solution containers 6 in a one-to-one correspondence;

[0040] The control unit 5 is used to perform the following operations:

[0041] Generate an experimental strategy according to the received experimental requirements, wherein the experimental strategy includes aspiration strategy, injection strategy and reaction strategy;

[0042] The liquid aspiration strategy is sent to the pathway switching unit 1 and the injection unit 2 respectively, the pathway switching unit 1 is used to connect the common pipeline 11 with the target second branch pipeline 13 according to the liquid aspiration strategy, and the target second branch pipeline 13 is connected to the container of the target solution; the injection unit 2 is used to transfer the target solution to the liquid storage unit 3 after the common pipeline 11 is connected with the target second branch pipeline 13;

[0043] The injection strategy is sent to the pathway switching unit 1 and the injection unit 2 respectively, the pathway switching unit 1 is used to connect the common pipeline 11 with the first branch pipeline 12 according to the injection strategy, and the injection unit 2 is used to transfer the target solution from the liquid storage unit 3 to the reaction unit 4 after the common pipeline 11 is connected with the first branch pipeline 12;

[0044] The reaction strategy is sent to the reaction unit 4 so that the reaction unit 4 forms a target reaction environment inside the reaction unit 4 according to the reaction strategy.

[0045] The multifunctional liquid reaction device provided in the embodiment of the present application can realize functions such as automatic liquid inlet process, automatic digestion, automatic cleaning, automatic liquid discharge, result calculation, etc. through the control of the control unit. Two or more reagents can be added automatically and quantitatively and / or repeatedly as needed to mix, or stand or heat to a constant temperature. The reaction unit can be used for constant temperature heating digestion of the mixed liquid, water quality permanganate determination experiment, water quality ammonia nitrogen determination experiment, water quality total phosphorus determination experiment, etc.

[0046] In order to enable the solution to achieve in-situ reaction after entering the reaction unit, various adverse effects caused by solution transfer are reduced. The embodiment of the present application can provide that the reaction unit 4 includes an inlet end and an outlet end, and the inlet end and the outlet end are respectively configured with a pressure relief valve 7; the pressure relief valve 7 is communicatively connected to the control unit 5; the control unit 5 is used to send the reaction strategy to the pressure relief valve 7, so that the pressure relief valve 7 adjusts the on-off state according to the reaction strategy. Specifically, the reaction strategy includes an injection start instruction and an injection end instruction; the pressure relief valve is used to adjust to a conducting state after receiving the injection start instruction and to a blocking state after receiving the injection end instruction. Pressure relief valves are respectively arranged at both ends of the reaction unit, at the upper and lower ends of the reaction unit, respectively. When the reagent is introduced into the reaction unit, the upper and lower two pressure relief valves are opened at the same time. After the reagent injection is completed, the upper and lower two pressure relief valves are closed at the same time, so that a closed space is formed inside the reaction unit. The internal environment of the closed space is adjusted to the target reaction environment as needed, and the corresponding experimental reaction can be carried out.

[0047] In order to automatically identify the concentration of the solution in the reaction unit, the embodiment of the present application can also provide that the reaction unit includes a body of transparent material, the body is equipped with a photoelectric signal acquisition component, the photoelectric signal acquisition component is communicatively connected with the control unit 5, and the control unit 5 is used to judge the concentration of the reagent in the body according to the absorption of the target wavelength light by the reagent collected by the photoelectric signal acquisition component. In order to eliminate the influence of external light on the acquisition accuracy of the photoelectric signal acquisition component, the embodiment of the present application can also provide that the photoelectric signal acquisition component includes a light source 81 and a photocell 82 arranged relatively on the body, and the reaction unit 4 also includes a light-shielding shell (not shown in the figure) arranged on the outside of the body.

[0048] The different colors of the solution are caused by the particles (molecules or ions) in the solution selectively absorbing a certain color of light. If the light of various colors is transmitted to the same degree, the substance is colorless and transparent. If only a part of the wavelength of light is allowed to pass through and the other wavelengths of light are absorbed, the solution will show the color of the transmitted light, that is, the solution will show the color that is complementary to the light it absorbs. For example, copper sulfate solution appears blue because it absorbs yellow light in white light; potassium permanganate solution appears purple because it absorbs green light in white light.

[0049] Therefore, the device provided in the embodiment of the present application can determine the concentration of the reagent based on the ability of the reagent to absorb a certain fixed wavelength of light (absorbance).

[0050] The absorbance is calculated using the Lambert-Beer law

[0051] Lambert-Beer Law:

[0052] When a parallel light beam perpendicularly passes through a non-scattering light-absorbing substance, its absorbance (A) is directly proportional to the concentration (c) of the light-absorbing substance and the optical path length (b). Lambert-Beer's law is the basic law of light absorption, applicable to all electromagnetic radiations and all light-absorbing substances. By using reagents with different concentrations and obtaining the curve of the absorbance and concentration of the reagent, the concentration of the reagent can be inversely calculated based on the absorbance.

[0053] For example, when using the device provided by this application to measure the permanganate index concentration, calibration needs to be carried out first. For example, 4 concentration standard solutions are used, with concentrations of 0 mg / L, 5 mg / L, 10 mg / L, and 20 mg / L respectively. After the reaction, their absorbances are obtained at a wavelength of 525 nm, and 4 absorbances.

[0054] Then, with the absorbance as the horizontal axis and the concentration as the vertical axis, a coordinate system is established to calculate the corresponding relationship. This relationship curve is divided into 3 segments: 0 - 5 mg / L, 5 - 10 mg, and 10 - 20 mg / L, corresponding to 6 parameters: k1, b1, k2, b2, k3, and b3.

[0055] During the calculation, the control unit collects optoelectronic signals. At the end of the experiment, the host computer software records 1 group of optoelectronic signal data every 1 second, records 15 groups, calculates the mean value, and then calculates the absorbance. Four absorbances I1, I2, I3, and I4 are obtained. Then, by adding the known 0, 5, 10, and 20, 6 parameters of k1, b1, k2, b2, k3, and b3 for the three straight lines are obtained. The calibration is completed.

[0056] If the instrument absorbance obtained in a certain experiment satisfies I1 < I < I2, then substituting I into the curve C = k1 * I + b1 gives the permanganate index concentration of the solution.

[0057] Since different experiments have different requirements for the temperature in the reaction unit, in order to automatically adjust the temperature in the reaction unit, the embodiment of this application can provide that the reaction unit includes a temperature control component 9. The temperature control component 9 is communicatively connected to the control unit 5. The temperature control component 9 is used to adjust the temperature in the reaction unit 4 according to the reaction strategy so as to form a target reaction environment inside the reaction unit 4. The temperature control component 9 can include an electric heating wire, a thermistor, a temperature sensor, a cooling fan, etc. The electric heating wire and thermistor are used to achieve heating, the temperature sensor is used to detect the temperature in the reaction unit in real time, and the cooling fan can cool the inside of the reaction unit as needed. Through this temperature control component, it can be ensured that the reaction unit is always at the optimal reaction temperature, thereby achieving effects such as improving the reaction efficiency and reaction quality.

[0058] In practical applications, the type of the passage switching unit can be selected as needed. For example, in one implementation, the embodiment of the present application can provide that the passage switching unit 1 includes a multi-channel switching valve island including a switching power component, and the multi-channel switching valve island includes a sapphire valve core and a valve head with a polytrifluoroethylene corrosion-resistant layer. The valve hole positioning of the multi-channel switching valve island adopts a code disk optical coupling method for positioning, and the switching power component includes a planetary reduction box motor.

[0059] The multi-channel switching valve island provided in the embodiment of the present application has an integrated motor control circuit inside, which controls the operation of the stepper motor by receiving instructions from the control unit CPU, thereby realizing the switching of the fluid path. The switching valve adopts a sapphire valve core, which can be applied to various corrosive liquids. The valve head is made of PCTFE (polytrifluoroethylene) and 316 stainless steel, which is maintenance-free. And the liquid contact surface does not contact stainless steel. The valve core adopts a multi-directional self-adaptive plane fitting method, which can effectively extend the life of the product. The valve body rotation adopts the NMB imported planetary reduction box motor as the power device, which is extremely reliable. The valve hole positioning adopts the code disk optical coupling method to effectively solve the problem of inaccurate positioning after the reduction box is worn; the drive module adopts a low-power drive chip, which can effectively reduce the heat generated during the operation of the chip to affect the performance. The performance indicators of the multi-channel switching valve island provided in the embodiment of the present application are shown in Table 1:

[0060] Table 1

[0061]

[0062]

[0063] In order to improve the injection accuracy during automatic injection, the embodiment of the present application can also provide that the injection unit 2 includes a vertical injection pump; the vertical injection pump is respectively connected to the liquid storage unit 3 and the pure water supply unit including the pure water valve 10; the liquid storage unit includes a liquid storage ring. The vertical injection pump can provide the function of accurately sucking and injecting reagents. The performance indicators of the vertical injection pump provided in the embodiment of the present application are shown in Table 2.

[0064] Table 2

[0065]

[0066]

[0067] In order to achieve precise control of each unit, the embodiment of the present application can also provide that the control unit includes an STM32F373CBT6 single-chip microcomputer, and the control unit is also connected to an audible and visual alarm component.

[0068] See also Figure 2 , Figure 3 , Figure 4 , Figure 5 , U5 is the STM32F373CBT6 microcontroller, which is the main control chip of the lower computer of the entire embedded hardware system. The upper computer reset circuit composed of R16 and C13 and X1 (8MHz active crystal oscillator) constitute the minimum system to ensure that the microcontroller can work normally.

[0069] Photo sen is a photosensitive device, U7 (OPA2156IDR) and U8 (OPA2192ID) are high-precision operational amplifiers. When the photosensitive device senses light of a central wavelength of a specific frequency, the photosensitive device converts the light intensity into a small current, which is converted into a voltage through R23. After passing through the circuit formed by U7 and U8, the voltage is amplified and conditioned, and then output to the analog voltage acquisition port of the microcontroller. Among them, R24 and D18 (BAT54S) constitute a voltage clamp and current limiting circuit, which can protect the safety of the analog voltage acquisition port of the microcontroller.

[0070] U11 is an OPA2192ID operational amplifier, NTC100K thermistor and R31 to form a voltage divider temperature detection circuit. After passing through the U11A voltage follower and R43 current limiting resistor, it is transmitted to the analog signal acquisition input of the STM32F373C8T6; after passing through the internal algorithm of the STM32F373C8T6, the voltage value is converted into the actual temperature value. The core device of the heating unit is the high-power MOS tube IRF3205ZS. The temperature loop uses the temperature collected by NTC100K as input and the 2KHz PWM duty cycle as output. The microcontroller controls the on and off of the MIOS tube through the 2KHz PWM switching frequency of the pin, drives the heating wire to heat, and forms a temperature closed loop with the NTC100K. The internal PID algorithm of the STM32F373C8T6 realizes precise constant temperature control, providing a precise temperature environment for chemical reactions.

[0071] U10, U9 two K78U05 (3-terminal voltage regulator chip) form a dual-rail power supply to power the operational amplifier, C35, C36, C37, C39 filter the output voltage to make the output voltage more stable;

[0072] U1 (LM2596) and U2 (LT1117) form a power module, which converts 24V power into 5V and 3.3V to power the microcontroller and other peripheral components.

[0073] U3 (SP3485) is a TTL to 485 communication chip that enables communication between the main control board and the host computer. D8, D9, and D11 are TVS diodes that can effectively protect the communication interface and avoid external interference.

[0074] U6 (SP3232EEN) is a serial communication chip, which is matched with C11, C12, C14, and C15 to form a serial communication unit, including 2 serial ports, which communicate with the vertical syringe pump and the multi-channel switching valve guide respectively. Therefore, the STM32F373C8T6 can send serial port instructions through this serial port unit to control the vertical syringe pump and the multi-channel switching valve guide to realize the automatic liquid inlet function.

[0075] U4 (MB85RC16PNF-G-JNE1) is an external storage chip that can store data without losing data during power failure.

[0076] The switching circuit composed of Q1, Q5, Q10, and Q12 (MOS tubes) controls the solenoid valves for pure water, pressure release, heating, and heat dissipation respectively.

[0077] D16 (light emitting diode), D17 (light emitting diode) and LS1 (buzzer) constitute an audible and visual alarm circuit. When an abnormal situation occurs, the microcontroller will sound an alarm.

[0078] The sound and light alarm assembly provided in the embodiment of the present application may include a sound (buzzer) alarm and a light (red LED) alarm. When the reagent liquid is replaced, the LED emits a "beep" sound to prompt the operator that it is completed.

[0079] When the water sample replacement is completed, the LED emits a "beep" sound to prompt the operator that it is completed.

[0080] When the cleaning action is completed, the LED emits a "beep" sound to prompt the operator that it is complete.

[0081] When the cooling is completed, the LED emits a "beep" sound to prompt the operator that it is completed.

[0082] When the cooling is shut down, the LED will emit a beep to remind the operator that it is complete.

[0083] When the digestion is completed, the LED emits a "beep" sound to prompt the operator that it is complete.

[0084] When the shutdown digestion is completed, the LED emits a "beep" sound to prompt the operator that it is complete.

[0085] When the temperature of the digestion tank is too high, the LED will sound an alarm and light up.

[0086] The present invention can be understood as inventing a set of solutions. In order to illustrate the effect brought by the method of the present invention, the water quality permanganate index determination experiment is taken as an example and this solution is used for determination.

[0087] 1. The injection pump and valve guide used in the device of the present invention have the function of being controlled by serial port instructions, so the device has a high degree of automation. Through CPU instructions, fully automatic liquid inlet, cleaning, liquid discharge, bubbling and other functions can be realized, which greatly improves work efficiency.

[0088] Commonly used serial port commands:

[0089] Valve pilot reset (except checksum): 0xCC, 0x02, 0x45, 0x00, 0x00, 0xDD;

[0090] Valve pilot selection channel 1 detection pool pipeline (except check code): 0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD;

[0091] Valve pilot selection channel 2 waste liquid pool pipeline (excluding check code): 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD;

[0092] Valve pilot selection channel 3 reserved pipeline (except check code): 0xCC, 0x02, 0x44, 0x03, 0x00, 0xDD;

[0093] Valve pilot selection channel 4 water sample pipeline to be tested (excluding check code): 0xCC, 0x02, 0x44, 0x04, 0x00, 0xDD;

[0094] Valve pilot selection channel 5 reserved pipeline (except check code): 0xCC, 0x02, 0x44, 0x05, 0x00, 0xDD;

[0095] Valve guide selection channel 6 potassium permanganate solution pipeline (excluding check code): 0xCC, 0x02, 0x44, 0x06, 0x00, 0xDD;

[0096] Valve pilot selection channel 7 reserved pipeline (except check code): 0xCC, 0x02, 0x44, 0x07, 0x00, 0xDD;

[0097] Valve pilot selection channel 8 reserved pipeline (except check code): 0xCC, 0x02, 0x44, 0x08, 0x00, 0xDD;

[0098] Valve guide selection channel 9 (1+3) sulfuric acid pipeline (excluding check code): 0xCC, 0x02, 0x44, 0x09, 0x00, 0xDD;

[0099] Valve pilot selection channel 10 air line (excluding check code): 0xCC, 0x02, 0x44, 0x0A, 0x00, 0xDD;

[0100] The syringe pump advances 0xAABB steps (excluding the checksum): 0xCC, 0x01, 0x41, 0xBB, 0xAA, 0xDD;

[0101] The syringe pump retreats 0xAABB steps (excluding the checksum): 0xCC, 0x01, 0x42, 0xBB, 0xAA, 0xDD;

[0102] Syringe pump reset (except checksum): 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD;

[0103] 2. High-precision syringe pump, with a minimum liquid inlet accuracy of 0.002 5mm / 1.038 1μL, ensures the accuracy of the liquid inlet volume. Combined with the multi-channel switching valve guide, the sequential injection and discharge of reagents in the experiment is more flexible and efficient.

[0104] 3. In-situ heating technology makes the reagents safer and more convenient during the heating process. Constant temperature heating can be performed according to the set temperature and time without moving the position.

[0105] In order to illustrate the practical effect of the device provided by this application, Figure 6 As shown in the figure, taking the water quality permanganate index experiment as an example, this paper introduces how the device can realize a fully automatic workflow in this experiment:

[0106] Step 1: Power on the instrument, initialize the system, reset the syringe pump (serial command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), reset the valve guide (serial command: 0xCC, 0x02, 0x45, 0x00, 0x00, 0xDD), open the water purification valve, and advance the syringe pump 4815 steps (serial command: 0xCC, 0x01, 0x41, 0xCF, 0x12, 0xDD), draw 5mL of deionized water, close the water inlet valve, switch the valve guide to the waste liquid pool pipeline (serial port command 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), reset the injection pump (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), pump 5mL of deionized water into the liquid storage loop, drain the residual liquid in the liquid storage loop, and fill it with deionized water.

[0107] Step 2: Liquid discharge action: the valve guide switches to the detection pool pipeline (0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD), the pressure relief valve is opened, the syringe pump advances 3274 steps (serial port command: 0xCC, 0x01, 0x41, 0xCA, 0x0C, 0xDD), extracts 3.4mL of waste liquid, closes the pressure relief valve, the valve guide switches to the waste liquid pool pipeline (serial port command 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the syringe pump is reset (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and the waste liquid is injected into the waste liquid pool. The liquid discharge action is cycled 3 times to empty the waste liquid pool. System initialization is completed.

[0108] Step 3: Select to add (1+3) sulfuric acid: reset the valve guide (serial port command: 0xCC, 0x02, 0x45, 0x00, 0x00, 0xDD), open the water purification valve, advance the injection pump 4815 steps (serial port command: 0xCC, 0x01, 0x41, 0xCF, 0x12, 0xDD), absorb 5 ml of deionized water, close the water purification valve, switch the valve guide to the waste liquid pool pipeline (serial port command 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), reset the injection pump (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and fill the storage ring with deionized water. The valve guide switches to the (1+3) sulfuric acid pipeline (serial command 0xCC, 0x02, 0x44, 0x09, 0x00, 0xDD), the syringe pump moves forward 2408 steps (serial command: 0xCC, 0x01, 0x41, 0x68, 0x09, 0xDD), and absorbs 2.5mL (1+3) sulfuric acid. The valve guide switches to the waste liquid pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the syringe pump moves back 193 steps (serial command: 0xCC, 0x01, 0x42, 0xC1, 0x00, 0xDD), and injects 0.2mL (1+3) sulfuric acid into the waste liquid pool. In the liquid pool, the valve guide switches to the detection pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD), the injection pump retreats 1926 steps (serial port command: 0xCC, 0x01, 0x42, 0x07, 0x86, 0xDD), and 2mL of sulfuric acid is injected into the detection pool. The valve guide switches to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump resets (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and 0.3mL of the remaining (1+3) sulfuric acid is injected into the waste liquid pool. The valve guide switches to the air line (serial command: 0xCC, 0x02, 0x44, 0x0A, 0x00, 0xDD), the syringe pump moves forward 482 steps (serial command: 0xCC, 0x01, 0x41, 0xE2, 0x01, 0xDD), and 0.5mL of air is drawn. The valve guide switches to the (1+3) sulfuric acid line (serial command: 0xCC, 0x02, 0x44, 0x09, 0x00, 0xDD), and the syringe pump moves back 96 steps ( Serial port command: 0xCC, 0x01, 0x42, 0x60, 0x00, 0xDD), inject 0.1mL of air into the (1+3) sulfuric acid pipeline, switch the valve guide to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), reset the injection pump (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and inject 0.4mL of remaining air into the waste liquid pool pipeline.

[0109] Step 4: Choose to add potassium permanganate solution: valve guide reset (serial port command: 0xCC, 0x02, 0x45, 0x00, 0x00, 0xDD), open the water purification valve, the injection pump advances 4815 steps (serial port command: 0xCC, 0x01, 0x41, 0x12, 0xCF, 0xDD), absorbs 5ml of deionized water, closes the water purification valve, switches the valve guide to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), resets the injection pump (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and fills the storage ring with deionized water. The valve guide is switched to the potassium permanganate solution pipeline (serial port command: 0xCC, 0x02, 0x44, 0x06, 0x00, 0xDD), the injection pump moves forward 2408 steps (serial port command: 0xCC, 0x01, 0x41, 0x68, 0x09, 0xDD), and 2.5mL of potassium permanganate solution is absorbed. The valve guide is switched to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump moves back 193 steps (serial port command: 0xCC, 0x01, 0x42, 0xC1, 0x00, 0xDD), and 0.2mL of potassium permanganate solution is injected. The liquid is transferred to the waste liquid pool, the valve guide is switched to the detection pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD), the injection pump retreats 1926 steps (serial port command: 0xCC, 0x01, 0x42, 0x68, 0x07, 0xDD), 2mL of potassium permanganate solution is injected into the detection pool, the valve guide is switched to the waste liquid pool pipeline (0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump is reset (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and the remaining potassium permanganate solution is transferred to the waste liquid pool. The valve guide switches to the air line (serial command: 0xCC, 0x02, 0x44, 0x0A, 0x00, 0xDD), the syringe pump moves forward 482 steps (serial command: 0xCC, 0x01, 0x41, 0xE2, 0x01, 0xDD), and 0.5mL of air is sucked. The valve guide switches to the potassium permanganate solution line (serial command: 0xCC, 0x02, 0x44, 0x06, 0x00, 0xDD), and the syringe pump moves back 9 In step 6 (serial port command: 0xCC, 0x01, 0x42, 0x60, 0x00, 0xDD), 0.1mL of air is injected into the potassium permanganate solution pipeline, the valve guide is switched to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump is reset (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and the remaining air is sent to the waste liquid pool pipeline.

[0110] Step 5: Select to fill the water sample to be tested: reset the valve guide (serial port command: 0xCC, 0x02, 0x45, 0x00, 0x00, 0xDD), open the water purification valve, advance the injection pump 4815 steps (serial port command: 0xCC, 0x01, 0x41, 0x12, 0xCF, 0xDD), absorb 5ml of deionized water (serial port command: 0xCC, 0x01, 0x41, 0xCF, 0x12, 0xDD), close the water purification valve, switch the valve guide to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), reset the injection pump (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and fill the storage ring with deionized water. The valve guide switches to the water sample pipeline to be tested (serial port command: 0xCC, 0x02, 0x44, 0x04, 0x00, 0xDD), the injection pump moves forward (serial port command: 0xCC, 0x01, 0x41, 0x68, 0x09, 0xDD), and draws 2.5mL of the water sample to be tested. The valve guide switches to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump retreats 193 steps (serial port command: 0xCC, 0x01, 0x42, 0xC1, 0x00, 0xDD) and injects 0.2mL of the water sample to be tested into the waste liquid pool, and the valve guide switches to the detection pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD).

[0111] The injection pump advances 1926 steps (serial port command: 0xCC, 0x01, 0x41, 0x86, 0x07, 0xDD), injects 2mL of the water sample to be tested into the detection pool, the valve guide switches to the waste liquid pool pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump is reset (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and injects 0.3mL of the remaining water sample to be tested into the waste liquid pool. The valve guide switches to the air line (serial port command: 0xCC, 0x02, 0x44, 0x0A, 0x00, 0xDD), the syringe pump moves forward 482 steps (serial port command: 0xCC, 0x01, 0x41, 0xE2, 0x01, 0xDD), 0.5 mL of air is drawn in, and the valve guide switches to the water sample line to be tested (serial port command: 0xCC, 0x02, 0x44, 0x04, 0x00, 0xDD).

[0112] The syringe pump moves back 96 steps, injects 0.1mL of air into the water sample pipeline to be tested, switches the valve guide to the waste liquid pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), resets the syringe pump (0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and injects the remaining air into the waste liquid pool pipeline. The reagent filling is completed.

[0113] Step 6: Turn on the heating, and when the temperature reaches the boiling water bath temperature, start recording the digestion time.

[0114] Step 7: When the digestion time reaches 30 minutes, measure the absorbance under visible light at a wavelength of 525 nm.

[0115] Step 8: Liquid discharge action: the valve guide switches to the detection pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD), the pressure relief valve is opened, the syringe pump advances 3274 steps (serial command: 0xCC, 0x01, 0x41, 0xCA, 0x0C, 0xDD), extracts 3.4mL of waste liquid, closes the pressure relief valve, the valve guide switches to the waste liquid pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the syringe pump is reset (serial command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and the waste liquid is injected into the waste liquid pool. The liquid discharge action is cycled 3 times to empty the waste liquid pool.

[0116] Step 9: Open the water purification valve, the injection pump moves forward 8186 steps (serial command: 0xCC, 0x01, 0x41, 0xFA, 0x1F, 0xDD), extracts 8.5mL of deionized water, the water inlet valve is closed, the valve guide is switched to the waste liquid pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the injection pump pumps out 0.2mL of deionized water to the waste liquid pool pipeline, and the valve guide is switched to the detection pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x01, 0x00,0xDD), the syringe pump retreats 7704 steps (serial port command: 0xCC, 0x01, 0x42, 0xBB, 0xAA, 0xDD), 8mL of deionized water is injected into the reaction tank, the valve guide is switched to the waste liquid tank pipeline (serial port command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the syringe pump is reset (serial port command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and the remaining 0.3mL of deionized water is injected into the waste liquid tank.

[0117] Step 10: Measure the absorbance at 525 nm wavelength of visible light.

[0118] Step 11: Liquid discharge action: the valve guide switches to the detection pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x01, 0x00, 0xDD), the pressure relief valve is opened, the syringe pump advances 3274 steps (serial command: 0xCC, 0x01, 0x41, 0xCA, 0x0C, 0xDD), extracts 3.4mL of waste liquid, closes the pressure relief valve, the valve guide switches to the waste liquid pool pipeline (serial command: 0xCC, 0x02, 0x44, 0x02, 0x00, 0xDD), the syringe pump is reset (serial command: 0xCC, 0x01, 0x45, 0x00, 0x00, 0xDD), and the waste liquid is injected into the waste liquid pool. The liquid discharge action is cycled 3 times to empty the waste liquid pool. The reagent filling process is completed.

[0119] This whole process includes

[0120] (1) Automated sequential liquid introduction process of potassium permanganate solution, water sample to be tested, and 1+3 sulfuric acid;

[0121] (2) High temperature water bath, 30 minutes digestion process;

[0122] (3) Automatic photoelectric limit number collection and recording process when digestion is completed;

[0123] (4) Automatic cooling process after digestion is completed;

[0124] (5) Automatic drainage process;

[0125] (6) Automatic zero sample injection, cleaning, comparison, and digestion process;

[0126] (7) Zero sample discharge process.

[0127] The entire process is operated by the host computer and is highly automated. Each decomposed action has a corresponding menu operation function and data is automatically recorded and analyzed.

[0128] In summary, the multifunctional liquid reaction device provided by the present application can provide an accurate, automatic and efficient experimental device for chemical detection experiments. It can realize functions such as accurate liquid inlet, multiple liquids participating in the reaction, constant temperature heating (time, temperature controllable, settable), reaction and automatic processing of experimental results. It can realize accurate quantitative automatic injection and automatic cleaning of multiple reagents. The use of in-situ constant temperature heating technology and PID constant temperature heating technology ensures more accurate temperature control and can also improve efficiency and accuracy. It is worth promoting and using on a large scale.

[0129] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, the elements defined by the sentence "comprise a ..." do not exclude the existence of other identical elements in the process, method, article or device including the elements.

[0130] The above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention are included in the protection scope of the present invention.

Claims

1. A multifunctional liquid reaction device, characterized in that: It comprises a passage switching unit, an injection unit, a liquid storage unit, a reaction unit and a control unit; the injection unit is connected to the liquid storage unit; the passage switching unit, the injection unit and the reaction unit are respectively connected to the control unit in a communicable manner; The passage switching unit includes a common pipeline, a first branch pipeline and a plurality of second branch pipelines; the liquid storage unit is connected to the common pipeline, and the reaction unit is connected to the first branch pipeline; the second branch pipelines are respectively used to be connected to different solution containers in a one-to-one correspondence; the passage switching unit includes a multi-channel switching valve island containing a switching power component, and the multi-channel switching valve island includes a sapphire valve core and a valve head with a polytrifluoroethylene corrosion-resistant layer; the valve hole positioning of the multi-channel switching valve island adopts a code disk optical coupling method for positioning, and the switching power component includes a planetary reduction box motor; The control unit is used to perform the following operations: Generate an experimental strategy according to the received experimental requirements, wherein the experimental strategy includes aspiration strategy, injection strategy and reaction strategy; The liquid aspiration strategy is sent to the pathway switching unit and the injection unit respectively, the pathway switching unit is used to connect the common pipeline with the target second branch pipeline according to the liquid aspiration strategy, and the target second branch pipeline is connected to the container of the target solution; the injection unit is used to transfer the target solution to the liquid storage unit after the common pipeline is connected with the target second branch pipeline; The injection strategy is sent to the pathway switching unit and the injection unit respectively, the pathway switching unit is used to connect the common pipeline with the first branch pipeline according to the injection strategy, and the injection unit is used to transfer the target solution from the liquid storage unit to the reaction unit after the common pipeline is connected with the first branch pipeline; Sending the reaction strategy to the reaction unit so that the reaction unit forms a target reaction environment inside the reaction unit according to the reaction strategy; The reaction unit comprises an inlet end and an outlet end, wherein the inlet end and the outlet end are respectively provided with a pressure relief valve; the pressure relief valve is communicatively connected with the control unit; the control unit is used to send the reaction strategy to the pressure relief valve so that the pressure relief valve adjusts the on-off state according to the reaction strategy; The reaction strategy includes an injection start instruction and an injection end instruction; the pressure relief valve is used to adjust to a conducting state after receiving the injection start instruction and to adjust to a blocking state after receiving the injection end instruction.

2. The multifunctional liquid reaction device according to claim 1, characterized in that: The reaction unit includes a main body made of a transparent material, on which a photoelectric signal acquisition component is disposed. The photoelectric signal acquisition component is communicatively connected to the control unit, and the control unit is used to judge the concentration of the reagent in the main body based on the absorption of target wavelength light by the reagent collected by the photoelectric signal acquisition component.

3. The multifunctional liquid reaction device according to claim 2, characterized in that: The photoelectric signal collection component includes a light source and a photocell which are arranged relatively on the main body, and the reaction unit also includes a light shielding shell which is arranged outside the main body.

4. The multifunctional liquid reaction device according to claim 1, characterized in that: The reaction unit includes a temperature control component, which is communicatively connected to the control unit. The temperature control component is used to adjust the temperature inside the reaction unit according to the reaction strategy so as to form a target reaction environment inside the reaction unit.

5. The multifunctional liquid reaction device according to claim 1, characterized in that: The injection unit comprises a vertical injection pump; the vertical injection pump is respectively connected to the liquid storage unit and a pure water supply unit including a pure water valve; the liquid storage unit comprises a liquid storage ring.

6. The multifunctional liquid reaction device according to claim 1, characterized in that: The control unit includes an STM32F373CBT6 single chip microcomputer, and the control unit is also connected to an audible and visual alarm component.

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