A blood separation method and a separation device

Abstract

The application discloses a blood separation method and a separation device, relates to the field of blood separation, and needs to use a blood separation device, wherein the blood separation device comprises a centrifugal device and a separation tube; the separation tube is in circular motion with the rotation center of the centrifugal device as the center during use; the separation tube comprises a blood inlet, a plasma outlet, a red blood cell outlet and a nucleated cell outlet; the plasma outlet is located at one end close to the rotation center; the red blood cell outlet and the nucleated cell outlet are located at one end away from the rotation center; blood is continuously injected into the blood inlet after the movement of the separation tube, and the plasma, the red blood cells and the nucleated cells in the separation tube continuously or intermittently flow out when the volumes of the plasma, the red blood cells and the nucleated cells reach a separation threshold value during the period. Compared with the prior art, the application adopts a continuous centrifugal method to separate various components in blood by guaranteeing a centrifugal sedimentation distance, so that the separation effect is obviously improved, and the actual effect is very remarkable.

Description

TECHNICAL FIELD

[0001] The present application relates to a medical device and a method of using the same, in particular to a blood separation device and a method of using the same. BACKGROUND

[0002] The mixture formed by collecting blood in a blood collection bag is called whole blood, which includes all components of blood cells and plasma. There are many disadvantages in whole blood transfusion, and modern blood transfusion advocates not using or less using whole blood. At the same time, part of the nucleated cells in whole blood can be used for cell transplantation treatment, so it is necessary to separate whole blood. The main components in whole blood are:

[0003] Relative density of cells

[0004] Red blood cells 1.09-1.11

[0005] Eosinophil 1.09-1.095

[0006] Neutrophil 1.080-1.085

[0007] Lymphocytes 1.062-1.077

[0008] Monocytes 1.050-1.066

[0009] Natural killer cells 1.050-1.070

[0010] Platelets 1.030-1.060

[0011] Plasma 1.025-1.030

[0012] Among them, eosinophils, basophils, lymphocytes, monocytes and natural killer cells are all white blood cells and all nucleated cells. These cells are collectively called white blood cells, and all white blood cells can also be called nucleated cells because red blood cells do not have cell nuclei.

[0013] As can be seen from the above, the relative densities of plasma, red blood cells and nucleated cells are different, so the main method for separating whole blood in the prior art is centrifugation. The principle of centrifugation is that after the whole blood is centrifuged by a centrifuge, the components of the whole blood are separated into a plasma layer and a red blood cell layer in the blood bag due to the difference in specific gravity. After the layered whole blood is squeezed or siphoned, the plasma in the upper layer of the blood bag is transferred to the plasma bag, and then SAGM (preservative) is added to the remaining red blood cell mother bag after the plasma is separated. After the blood bag conduit is heat sealed and broken, the whole blood is successfully separated into plasma and red blood cell suspension.

[0014] Umbilical cord blood is the blood remaining in the placenta and umbilical cord after the fetus is delivered, the umbilical cord is ligated and separated, contains hematopoietic stem cells that can rebuild the human hematopoietic and immune system, and can be used for hematopoietic stem cell transplantation to treat more than 80 diseases. Therefore, umbilical cord blood becomes an important source of hematopoietic stem cells, especially a source of non-blood-related hematopoietic stem cells. It is also a very important human biological resource. The main costs of umbilical cord blood are red blood cells, nucleated cells (hematopoietic stem cells belong to one of the nucleated cells) and plasma, and the volume of hematopoietic stem cells in umbilical cord blood accounts for less than 1% of the total volume of umbilical cord blood. At the same time, hematopoietic stem cells need to be stored in liquid nitrogen, and the cost of long-term liquid nitrogen storage is very high. In order to improve the storage capacity, the umbilical cord blood needs to be removed part of the plasma, concentrated and then frozen.

[0015] The commonly used nucleated cell separation means are three, which are triple blood bag + centrifuge, AXP automatic preparation system and SEPAX preparation system, but the above separation means still has several defects. The first is that due to the quality difference of the centrifuge and the proficiency of the operator, there are different degrees of residual red blood cells in the separated plasma and excessive plasma in the red blood cells. The second is that due to the similar specific gravity of nucleated cells and red blood cells, red blood cells and nucleated cells cannot be effectively separated, so that the separation rate of nucleated cells is low, and a large amount of nucleated cells are left in red blood cells. The third is that in order to ensure that the stem cells are not lost too much, the red blood cells are selected to be less, and the final concentration volume is usually 20-30ml. The large concentration volume means that more cryoprotective agents are needed, and more space is occupied. SUMMARY

[0016] The present application adopts the method of continuous centrifugation and continuous collection to ensure the stability of the blood layering in the centrifugal device, and solves the problems of mixed nucleated cells in red blood cells and low collection rate of nucleated cells by the gravity separation method of red blood cells and nucleated cells. The collection rate of the device and method of the present application reaches more than 90%, and theoretically can reach 99%, which greatly reduces the concentration volume of nucleated cells to 5-10ml, significantly reduces the storage cost, and has excellent practical use effect.

[0017] In order to solve the above technical problems, the present application solves the above technical problems by the following technical scheme: a blood separation method, which needs to use a blood separation device, the blood separation device includes a centrifugal device and a separation tube, and the separation tube makes a circular motion with the center of the centrifugal device as the center when in use; the separation tube includes a blood inlet, a plasma outlet, a red blood cell outlet and a nucleated cell outlet, the plasma outlet is located at one end close to the center of rotation, and the red blood cell outlet and the nucleated cell outlet are located at one end away from the center of rotation; after the movement of the separation tube, blood is continuously injected into the blood inlet, and when the volume of plasma, red blood cells and nucleated cells in the separation tube reaches the separation threshold, they continuously or intermittently flow out.

[0018] Preferably, the nucleated cell outlet is provided with sensors on both sides, which determine the red blood cell and nucleated cell separation threshold.

[0019] A blood separation device comprises a centrifugal device and a separation tube, the separation tube comprising a blood inlet, a plasma outlet, a red blood cell outlet and a nucleated cell outlet, the plasma outlet being located at one end close to the rotation center, the red blood cell outlet and the nucleated cell outlet being located at one end away from the rotation center; the separation tube is inclinedly arranged, the end with the red blood cell outlet and the nucleated cell outlet being higher than the end with the plasma outlet, the nucleated cell outlet being higher than the red blood cell outlet, and the nucleated cell outlet being provided with sensors on both sides.

[0020] Preferably, the sensors are light sensors.

[0021] Preferably, flow valves are arranged on the blood inlet, the plasma outlet, the nucleated cell outlet and the red blood cell outlet.

[0022] Preferably, the flow valves are adjustable peristaltic pumps.

[0023] Another blood separation device comprises a centrifugal device and a separation tube, the separation tube comprising a straight tube section close to the rotation center and a curved tube section away from the rotation center, the curved tube section being bent upward, the straight tube section being provided with a blood inlet and a plasma outlet closer to the rotation center than the blood inlet, the curved tube section being provided with a nucleated cell outlet and a red blood cell outlet farther away from the rotation center than the nucleated cell outlet, and the nucleated cell outlet being provided with sensors on both sides.

[0024] Preferably, the straight tube section is arranged horizontally.

[0025] Preferably, the curved tube section is bent by 90° in a circular arc, and the straight tube section is in the tangent direction of the curved tube section.

[0026] Preferably, the sensors are light sensors.

[0027] Preferably, flow valves are arranged on the blood inlet, the plasma outlet, the nucleated cell outlet and the red blood cell outlet.

[0028] Preferably, the flow valves are adjustable peristaltic pumps.

[0029] Preferably, the plasma outlet is located at the side end of the straight tube section close to the rotation center, the red blood cell outlet is located at the side end of the curved tube section away from the rotation center, the nucleated cell outlet is located on the upper part of the curved tube section, and the blood inlet is located on the upper part of the straight tube section.

[0030] Preferably, the blood inlet is higher than the plasma outlet, and the nucleated cell outlet and the red blood cell outlet are higher than the plasma outlet.

[0031] When a cell suspension is centrifuged, the settling rate of the cells is directly proportional to the centrifugal force. The physical properties of the solution also affect the settling rate. At a fixed centrifugal force and liquid viscosity, the settling rate is directly proportional to the size of the particle and the difference between its own density and the density of the surrounding medium. The settling equation for a sphere in a centrifugal field is: V = d 2 (ρ - ρ0)g / θη, where V is the settling velocity, d is the cell diameter, ρ is the cell density, ρ0 is the medium density, g is the centrifugal force, θ is the ratio of the cell's friction coefficient to that of an equivalent sphere, and η is the absolute viscosity of the medium.

[0032] From the above formula, it can be seen that the density of blood cells is larger than that of plasma. During centrifugation, red blood cells and nucleated cells will move to the bottom. However, there is also a tendency for nucleated cells to concentrate in the upper layer, because the diameter and density of nucleated cells are smaller, and the settling velocity is small, while the diameter and density of red blood cells are larger, and the settling velocity is larger than that of nucleated cells. The rapid settling of red blood cells causes the surrounding liquid to move upward, driving white blood cells upward. The faster the red blood cells descend, the more conducive it is to the upward movement of the surrounding liquid. The cell concentration, the volume ratio of cells to the entire liquid, the greater the cell concentration, the closer the distance between red blood cells and white blood cells, the more conducive to convection. The longer the settling distance, the more the convection can fully play its role.

[0033] However, for one-time blood separation, there are the following problems: 1. To speed up the descent of red blood cells, only the centrifugal force can be increased, which requires high centrifuge and separation equipment, greatly increasing the cost. 2. Cell concentration is a fixed property of blood and cannot be changed. 3. The settling distance is different at different positions from the bottom, and the settling distance is different and cannot be changed. When the centrifuge bag / tube is filled with blood, the cells in the upper part are separated well, and the cells in the lower part are separated poorly. The existing one-time blood separation equipment can only increase the centrifugal power and the size of the separator to increase efficiency.

[0034] The present application changes the one-time blood separation into continuous blood separation, completely overcomes the above problems, and simply speaking, uses different separation principles to make the above problems not appear. The basic concept of the present application can be understood as "continuous injection and intermittent outflow". When the separation tube is filled or the blood is stratified, if continuous injection of blood is still needed, the plasma, red blood cells and nucleated cells after stratification must be flowed out. In the present application, these are not flowed out at the same time, but are flowed out after reaching a preset threshold. Generally speaking, after the separation tube is filled or the blood is stratified, the plasma triggers the threshold, at which time the plasma is flowed out. If the plasma is flowed out, the volume of red blood cells and nucleated cells in the separation tube becomes larger and larger. As long as the volume of red blood cells and nucleated cells triggers the threshold, the red blood cells and nucleated cells are also flowed out. Under the continuous or intermittent outflow of plasma, red blood cells and nucleated cells, the volume of red blood cells and nucleated cells in the separation tube is always at the optimal absorption volume. Again, because of the shape and placement angle of the separation tube in the present application, the separation of red blood cells and nucleated cells is quite thorough, and red blood cells and nucleated cells are basically not mixed. As long as the blood is not exhausted, and the plasma, nucleated cells and red blood cells are orderly taken under the monitoring of the light sensor and the dynamic adjustment of the flow valve, the stratification of the plasma, nucleated cells and red blood cells in the separation tube is always stable, and the purity of the collected plasma, red blood cells and nucleated cells can be guaranteed. The collection method in the present application improves the collection rate of nucleated cells to more than 90%~95%, and theoretically can reach 99% if the separation tube is small enough, far exceeding the current collection rate, while also greatly reducing the collection amount. In the present application, only 5ml to 10ml of amount needs to be collected to collect most of the nucleated cells in 200ml, greatly reducing the cost of low-temperature storage of nucleated cells. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 It is the principle diagram of the embodiment 1 of the present application.

[0036] Figure 2 It is the principle diagram of the embodiment 2 of the present application. DETAILED DESCRIPTION

[0037] Embodiment 1: A blood separation device, comprising a centrifugal device and a separation tube, generally a placement table is provided on the centrifugal device, and the placement table moves with the centrifugal shaft in the centrifugal device. The separation tube is arranged on the placement table, and a blood bag, a plasma bag, a collection bag and a red blood cell bag are also arranged on the placement table. The volume of the separation tube is smaller than the volume of the blood to be centrifuged, generally the volume of the separation tube is between 10ml and 20ml, and the separation tube is a straight tube. If necessary, it can also be increased or decreased. Basically, the volume of the separation tube is one-tenth to one-fifth of the volume of the blood to be separated. In fact, when the volume of the separation tube is more than one-tenth of the volume of the blood to be separated, the collection effect is better, but the total separation time is longer. In the present application, the centrifugal shaft can be regarded as the center of rotation in the centrifugal motion.

[0038] The separation tube comprises a blood inlet, a plasma outlet, a red blood cell outlet and a nucleated cell outlet, all of which are provided with flow valves, which are adjustable peristaltic pumps. The separation tube is arranged at an angle of 0° to 60°, preferably 15° to 45°. The plasma outlet is located at one end close to the center of rotation, and the red blood cell outlet and the nucleated cell outlet are located at the other end away from the center of rotation; the nucleated cell outlet is higher than the red blood cell outlet. The end with the red blood cell outlet and the nucleated cell outlet is higher than the end with the plasma outlet, and the blood inlet is close to the end close to the center of rotation and between the plasma outlet and the nucleated cell outlet. At the same time, the position of the nucleated cell outlet needs to be accurately designed, and the position of the nucleated cell outlet must be determined according to the volume ratio of the nucleated cells in the blood to ensure that the nucleated cell accumulation area after blood separation is at the nucleated cell outlet.

[0039] The nucleated cell outlet is provided with sensors on both sides, which are light sensors. The position of the light sensor also needs to be accurately designed according to the volume ratio of the nucleated cells in the blood, and the light sensor is usually located at the separation of the nucleated cells and the red blood cells, and the other light sensor above the nucleated cell outlet mainly detects the thickness of the nucleated cell layer.

[0040] When the separation tube is inclined at an angle of 15°, the separation principle is as follows: blood enters from the blood inlet, and the gravity and centrifugal force form a resultant force, and the blood cells move along the direction of the resultant force under the action of the resultant force, that is, towards the bottom of the separation tube. Red blood cells move faster, and white blood cells move slower. When the red blood cells contact the lower part of the separation tube, the red blood cells will collide with the lower wall of the separation tube due to their large speed and inertia, and the white blood cells will be on the upper layer of the red blood cells. Because the density of red blood cells is greater than that of white blood cells, under the action of the resultant force, the red blood cells always remain at the bottom from the moment they contact the bottom of the separation tube to the moment they move towards the outlet. At this time, the blood in the separation tube is separated but not filled. With the continuous injection of blood, the plasma is continuously discharged from the plasma outlet, and the red blood cells gradually increase and accumulate at the bottom. When the red blood cells are detected by the lower light sensor of the nucleated cell outlet, the red blood cell outlet valve is opened, and the red blood cell accumulation volume is maintained unchanged. The red blood cell volume remains stable, and the white blood cell thickness slowly accumulates and thickens. When the white blood cells are detected by the upper light sensor of the nucleated cell outlet, the nucleated cell outlet is opened, and the nucleated cell thickness is maintained unchanged. When there is no blood flow into the blood inlet, the red blood cell layer and the white blood cell layer on the lower side of the separation tube will slowly become shorter under the action of the centrifugal force and deposit downward until all the red blood cells flow out of the outlet. After the white blood cells flow out of the nucleated cell outlet, the plasma continues to flow out until the required volume is collected. The separation is completed. During the above separation process, the separation tube is always not filled.

[0041] The method of separating nucleated cells using the blood separation device of Example 1, in this method, the nucleated cells to be collected are stem cells in umbilical cord blood. The volume of umbilical cord blood is more than 100 ml. The following steps are included, Step A: connecting the blood inlet to the blood bag, connecting the plasma outlet to the plasma bag, connecting the nucleated cell outlet to the collection bag, and connecting the red blood cell outlet to the red blood cell bag, then opening the blood inlet to allow blood to flow into the separation tube, the volume of blood flowing into the separation tube is one-half to two-thirds of the volume of the separation tube. Step B: rotating the centrifugal device to rotate the separation tube around the center of rotation, at this time the blood in the separation tube is stratified, after the blood is stratified, continue to flow into the blood, open the plasma outlet to allow the plasma to continue to flow into the plasma bag. Step C: when the red blood cell sensing optical sensor senses red blood cells, open the red blood cell outlet to allow red blood cells to flow into the red blood cell bag, when the red blood cell sensing optical sensor does not sense red blood cells, close the red blood cell outlet, and always keep the red blood cells in the separation tube at a stable stratification position. Step D: when the optical sensor sensing the thickness of the nucleated cells senses the nucleated cells, open the nucleated cell outlet to allow the nucleated cells to flow into the collection bag, when the optical sensor does not sense the nucleated cells, close the nucleated cell outlet. Repeat steps C and D until the blood is exhausted or the collection bag is full, and at the same time, blood continues to flow into the separation tube in steps C and D to keep the separation tube stratified at all times until the blood is exhausted or the collection bag is full. The stem cell collection rate collected by this method is more than 95%, the minimum collection volume is as low as 5 ml, and only a small amount of plasma will flow into the collection bag at the end, and the degree of stem cell contamination is very small.

[0042] Example 2: A nucleated cell separation device, comprising a centrifugal device, usually provided with a placement table on the centrifugal device, the placement table moves with the centrifugal shaft in the centrifugal device. The separation tube is arranged on the placement table, and the blood bag, plasma bag, collection bag, and red blood cell bag are also arranged on the placement table. The volume of the separation tube is less than the volume of the blood to be centrifuged, and the volume of the separation tube is usually between 10 ml and 20 ml, and can be increased or decreased as needed. In this application, the centrifugal shaft can be regarded as the center of rotation in the centrifugal motion.

[0043] The separation tube includes a straight tube section near the centrifugal shaft and a curved tube section away from the centrifugal shaft, and the straight tube section and the curved tube section are integrally connected. The straight tube section and the curved tube section can be smoothly transitioned or transitioned at an angle. The curved tube section is curved upward in a curve or a straight line. The straight tube section is horizontally arranged or has an angle with the horizontal plane, and if the straight tube section has an angle with the horizontal plane, it is best to incline the straight tube section upward, and the highest height does not exceed the lowest height of the curved tube section. Usually, the curved tube section is bent at an angle of 90°, the straight tube section is in the tangent direction of the curved tube section, and the straight tube section and the curved tube section are smoothly transitioned.

[0044] The straight pipe section is provided with a blood inlet and a plasma outlet closer to the centrifugal shaft, and the last plasma outlet is arranged on the outermost side wall of the straight pipe section. The curved pipe section is provided with a nucleated cell outlet and a red blood cell outlet farther away from the centrifugal shaft, and the red blood cell outlet is preferably arranged on the outermost side wall of the curved pipe section. At the same time, the position of the nucleated cell outlet needs to be accurately designed, and the position of the nucleated cell outlet must be determined according to the volume ratio of the nucleated cells in the blood to ensure that the nucleated cell aggregation area after blood separation is at the nucleated cell outlet. The curved pipe section is provided with two sets of light sensors, and the nucleated cell outlet is located between the light sensors. The position of the light sensor also needs to be accurately designed according to the volume ratio of the nucleated cells in the blood. Usually, the light sensor is located at the separation position of the nucleated cells, the plasma and the red blood cells. Further, the light sensor can be located at the position close to the plasma at the separation position of the nucleated cells and the plasma, and at the position close to the red blood cells at the separation position of the nucleated cells and the red blood cells. The blood inlet, the plasma outlet, the nucleated cell outlet and the red blood cell outlet are all provided with flow valves, and the flow valves are adjustable peristaltic pumps.

[0045] In this embodiment, the separation device is divided into two parts of a straight pipe section and a curved pipe section in communication. The straight pipe section is horizontally placed, and the curved pipe section is bent upward and away from the centrifugal shaft. Of course, the straight pipe section can also have a certain angle with the horizontal plane, but the curved pipe section must be bent upward. When the blood cells reach the joint of the straight pipe and the curved pipe, the red blood cells and the nucleated cells with large specific gravity are thrown in the curved pipe section during centrifugation. At this time, the speed of the red blood cells is greater than that of the white blood cells. According to the centrifugal force formula: F=mV2 / r, F-the centrifugal force of the cells, m-the mass of the cells, V-the speed of the cells, and r-the radius of the arc of the curved pipe section. The red blood cells have a larger mass than the white blood cells, and the speed is fast. All the red blood cells have a much larger force than the white blood cells on the lower part of the curved pipe. The white blood cells are in the upper layer of the red blood cells. At this time, the red blood cells are in the upper part of the curved pipe section, that is, the bottom of the whole separation pipe. The nucleated cells are in the lower part of the curved pipe section, that is, between the plasma and the red blood cells. In order to achieve better collection effect, the plasma extends to the curved pipe section. At this time, the nucleated cells, the red blood cells and the plasma are clearly separated. Because of the joint action of the centrifugal force and the gravity during rotation, the red blood cells always move upward, the nucleated cells always move downward at the red blood cell aggregation place and always move upward at the plasma aggregation place. At the same time, the design of the curved pipe and the straight pipe ensures the distance of the centrifugal sedimentation, which will not cause the mixing of part of the nucleated cells and the red blood cells, so that most of the nucleated cells are aggregated together, thereby better separation and greatly reduced collection difficulty.

[0046] The method of separating nucleated cells from blood using the blood separation device of Example 2, in this method, the nucleated cells to be collected are stem cells in umbilical cord blood. The amount of umbilical cord blood is more than 100 ml. The following steps are included, Step A: connecting the blood inlet to the blood bag, the plasma outlet to the plasma bag, the nucleated cell outlet to the collection bag, and the red blood cell outlet to the red blood cell bag, then opening the blood inlet to let the blood flow into the separation tube. Step B: rotating the centrifugal device to rotate the separation tube with the center of rotation, at this time the nucleated cells and red blood cells move to the elbow tube section, the plasma moves to the straight tube section, and the separation tube is filled with blood, the blood in the separation tube is stratified, the plasma is located in the straight tube section and extends to the elbow tube section, the red blood cells are located at the bottom of the elbow tube section, and the nucleated cells are located between the plasma and the red blood cells, at this time the light sensors on both sides of the nucleated cell outlet respectively sense the plasma and red blood cells. Step C: open the plasma outlet to continuously flow the plasma into the plasma bag. Step D: when the light sensor sensing the red blood cells senses the red blood cells, open the red blood cell outlet to let the red blood cells flow into the red blood cell bag, and when the light sensor sensing the red blood cells does not sense the red blood cells, close the red blood cell outlet, and always keep the red blood cells in the separation tube stably at the initial stratification position. Step E: when the light sensor sensing the plasma does not sense the plasma, open the nucleated cell outlet to let the nucleated cells flow into the collection bag, and when the light sensor sensing the plasma senses the plasma, close the nucleated cell outlet. Repeat Step C, Step D and Step E until the blood is exhausted or the collection bag is full, and at the same time, the blood continuously flows into the separation tube to fill the separation tube until the blood is exhausted or the collection bag is full. The stem cells collected by this method have a collection rate of more than 95%, the minimum collection amount is as low as 5 ml, and only a small amount of plasma will flow into the collection bag at the end, and the degree of stem cell contamination is very small.

Claims

1. Blood separation device comprising a centrifugation device and a separation tube, characterized in that, The separation tube comprises a blood inlet, a plasma outlet, a red blood cell outlet and a nucleated cell outlet, the plasma outlet is located at one end close to the rotation center, the red blood cell outlet and the nucleated cell outlet are located at one end away from the rotation center; the separation tube is arranged obliquely, one end with the red blood cell outlet and the nucleated cell outlet is higher than the other end with the plasma outlet, the nucleated cell outlet is higher than the red blood cell outlet, and inductors are arranged on both sides of the nucleated cell outlet; Flow valves are arranged on the blood inlet, the plasma outlet, the nucleated cell outlet and the red blood cell outlet, and the flow valves are adjustable peristaltic pumps.

2. The blood separation device of claim 1, wherein, The inductors are light inductors.

3. A blood separation device comprising a centrifuge and a separation tube, characterized in that, The separation tube comprises a straight tube section close to the rotation center and a curved tube section away from the rotation center, the curved tube section is bent upward, the straight tube section is provided with a blood inlet and a plasma outlet closer to the rotation center than the blood inlet, and the curved tube section is provided with a nucleated cell outlet and a red blood cell outlet farther away from the rotation center than the nucleated cell outlet, and inductors are arranged on both sides of the nucleated cell outlet. Flow valves are arranged on the blood inlet, the plasma outlet, the nucleated cell outlet and the red blood cell outlet, and the flow valves are adjustable peristaltic pumps.

4. The blood separation device of claim 3, wherein, The straight tube section is arranged horizontally.

5. The blood separation device of claim 3, wherein, The curved tube section is bent in a 90° circular arc, and the straight tube section is in the tangent direction of the curved tube section.

6. The blood separation device of claim 3, wherein, The inductors are light inductors.

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