Sample analyzer, signal acquisition method, and computer storage medium

Abstract

Embodiments of the present application disclose a sample analyzer, a signal acquisition method and a computer storage medium, so that the collected light signal does not cause adverse effects on the results of blood sedimentation analysis. Embodiments of the present application comprise: a control module controls the opening or closing of a light signal generation module, a light signal acquisition module and a heating module connected in communication therewith; the control module controls the light signal acquisition module to collect light signals outside the time period when the heating module brings interference signals; the collection of light signals can not be disturbed; the control module controls the light signal acquisition module to suspend the collection of light signals within the time period when the heating module brings interference signals, thereby avoiding the collection of disturbed light signals. Through the linkage control of the light signal acquisition module and other modules, pure and undisturbed light signals can be collected in the whole signal acquisition process, which is beneficial to the subsequent signal processing and analysis process.

Description

TECHNICAL FIELD

[0001] Embodiments of the present application relate to the field of blood sedimentation detection, and in particular to a sample analyzer, a signal acquisition method and a computer storage medium. BACKGROUND

[0002] In the field of blood detection and analysis, there are various detection instruments for blood detection, such as blood analyzers, glycated hemoglobin analyzers, and whole blood CRP analyzers. Blood detection equipment generally includes a heating module and a detection signal acquisition module. The detection signal acquisition module is used to acquire detection signals, such as light signals transmitted through the detection sample. The heating module is used to provide a suitable temperature for sample detection.

[0003] In order to save the cost and volume of the detection equipment, the heating module and the detection signal acquisition module are usually integrated on one device to save the space occupied by the equipment. In addition, for detection equipment that measures the erythrocyte sedimentation rate using the erythrocyte aggregation method, since the temperature affects the measurement of the erythrocyte aggregation method, a heating module with temperature control function is also needed to control the temperature to eliminate the influence of temperature on the measurement of the erythrocyte sedimentation rate. Therefore, the heating module and the detection signal acquisition module also need to be integrated on one device.

[0004] However, integrating the heating module and the detection signal acquisition module on one device also has disadvantages. The reason is that the opening and closing of the heating module will generate an opening and closing signal, which will interfere with the acquisition of the detection signal. To solve this problem, a zero-crossing opening and closing method is usually used to eliminate the interference of the peak voltage and current of the heating device on the acquisition of the detection signal. However, if this zero-crossing opening and closing method is used, a 220V to low voltage transformer is needed, which increases the complexity of the whole hardware system and is not conducive to the miniaturization and low cost of the instrument.

[0005] Therefore, there is an urgent need for a detection device that can realize miniaturization of the detection device and avoid interference of the heating module with the acquisition of the detection signal. SUMMARY

[0006] Embodiments of the present application provide a sample analyzer, a signal acquisition method and a computer storage medium, so that the acquired light signal does not adversely affect the blood sedimentation analysis result when used for blood sedimentation analysis.

[0007] The first aspect of the embodiments of the present application provides a sample analyzer, comprising a blood sedimentation detection module and a sampling distribution module.

[0008] The sampling distribution module is used to collect a blood sample and distribute the blood sample to the blood sedimentation detection module.

[0009] The blood sedimentation detection module comprises a detection pipeline, a light signal generation module, a light signal collection module, a heating module, and a control module connected with the light signal generation module, the light signal collection module, and the heating module;

[0010] The detection pipeline is used to provide a detection site for the blood sample.

[0011] The light signal generation device generates a light source and is used to irradiate the blood sample distributed in the detection pipeline with light;

[0012] The light signal collection module collects the light signal of the absorption or scattering of light by the blood sample distributed in the detection pipeline to detect the erythrocyte sedimentation rate of the blood sample.

[0013] The heating module heats the blood sample in the detection pipeline to maintain the blood in the detection pipeline within a preset temperature range; when the temperature of the blood in the detection pipeline is within the preset temperature range, the control module controls the heating module to be turned off to stop heating the blood; when the temperature of the blood in the detection pipeline is lower than the preset temperature range, the control module controls the heating module to be turned on to start heating the blood.

[0014] The control module controls the light signal collection module to collect the light signal of the absorption or scattering of light by the blood sample distributed in the detection pipeline to detect the erythrocyte sedimentation rate of the blood sample outside the time period when the heating module is turned on or turned off to bring an interference signal.

[0015] The second aspect of the embodiments of the present application provides a sample analyzer, comprising a blood sedimentation detection module and a sampling and distribution module.

[0016] The sampling and distribution module is used to collect a blood sample and distribute the blood sample to the blood sedimentation detection module.

[0017] The blood sedimentation detection module comprises a detection pipeline, a light signal generation module, a light signal collection module, a heating module, and a signal processing module.

[0018] The detection pipeline is used to provide a detection site for the blood sample.

[0019] The light signal generation device generates a light source and is used to irradiate the blood sample distributed in the detection pipeline with light;

[0020] The heating module heats the blood sample in the detection pipeline to maintain the blood in the detection pipeline within a preset temperature range.

[0021] The light signal collection module collects light signals of absorption or scattering of light by the blood sample distributed in the detection pipeline at a first frequency;

[0022] The signal processing module receives the light signals collected by the light signal collection module and converts the light signals into electrical signals, integrates the electrical signals to eliminate interference signals, and obtains the erythrocyte sedimentation rate of the blood sample according to the electrical signals after eliminating interference.

[0023] The third aspect of the embodiment of the present application provides a signal collection method, which is applied to the sample analyzer in the first aspect and includes the following steps:

[0024] Distributing a blood sample to be detected to a detection pipeline;

[0025] Heating the blood sample in the detection pipeline by a heating module to maintain the blood in the detection pipeline in a preset temperature range, turning off the heating module to stop heating the blood when the temperature of the blood in the detection pipeline is in the preset temperature range, and turning on the heating module to start heating the blood when the temperature of the blood in the detection pipeline is lower than the preset temperature range.

[0026] Outside the time period in which the heating module brings interference signals, collecting light signals of absorption or scattering of light by the blood sample distributed in the detection pipeline by the light signal collection module to detect the erythrocyte sedimentation rate of the blood sample.

[0027] The fourth aspect of the embodiment of the present application provides a signal collection method, which is applied to the sample analyzer in the second aspect and includes the following steps:

[0028] Distributing a blood sample to be detected to a detection pipeline;

[0029] Heating the blood sample in the detection pipeline by a heating module to maintain the blood in the detection pipeline in a preset temperature range;

[0030] Collecting light signals of absorption or scattering of light by the blood sample distributed in the detection pipeline at a first frequency by a light signal collection module;

[0031] Converting the collected light signals into electrical signals, integrating the electrical signals to eliminate interference signals, and obtaining the erythrocyte sedimentation rate of the blood sample according to the electrical signals after eliminating interference.

[0032] The fifth aspect of the embodiment of the present application provides a computer storage medium, and the computer storage medium stores instructions. When the instructions are executed on a computer, the computer executes the method of the third aspect.

[0033] From the above technical solutions, the embodiment of the present application has the following advantages:

[0034] In the embodiment of the present application, the control module is connected with the optical signal generation module, the optical signal acquisition module and the heating module, and controls the opening or closing of other modules connected therewith. The control module controls the optical signal acquisition module to acquire the optical signal outside the time period when the heating module brings the interference signal, and the acquisition of the optical signal can not be disturbed. Within the time period when the heating module brings the interference signal, the control module controls the optical signal acquisition module to suspend the acquisition of the optical signal, so as to avoid the acquisition of the disturbed optical signal. Through the linkage control of the optical signal acquisition module and other modules, the pure and undisturbed optical signal can be acquired in the whole signal acquisition process, which is beneficial to the subsequent signal processing and analysis process. BRIEF DESCRIPTION OF DRAWINGS

[0035] Figure 1 FIG. 1 is a structural schematic diagram of a sample analyzer in the embodiment of the present application;

[0036] Figure 2 FIG. 2 is a schematic diagram of an electrical signal acquired by the optical signal acquisition module in the embodiment of the present application;

[0037] Figure 3 FIG. 3 is another schematic diagram of an electrical signal acquired by the optical signal acquisition module in the embodiment of the present application;

[0038] Figure 4 FIG. 4 is another structural schematic diagram of a sample analyzer in the embodiment of the present application;

[0039] Figure 5 FIG. 5 is a flow schematic diagram of a signal acquisition method in the embodiment of the present application;

[0040] Figure 6 FIG. 6 is another flow schematic diagram of a signal acquisition method in the embodiment of the present application. DETAILED DESCRIPTION

[0041] The embodiment of the present application provides a sample analyzer, a signal acquisition method and a computer storage medium, so that the acquired optical signal does not adversely affect the blood sedimentation analysis result when used for blood sedimentation analysis.

[0042] Please refer to Figure 1 The sample analyzer in the embodiment of the present application comprises:

[0043] a blood sedimentation detection module 10 and a sampling distribution module 11.

[0044] The blood sedimentation detection module 10 is configured to detect the erythrocyte sedimentation rate of the blood sample, i.e., to detect the blood sedimentation. The sample dispensing module 11 is configured to suck the blood sample in the sample pool and dispense the sucked blood sample into the blood sedimentation detection module 10, so that the blood sedimentation detection module 10 detects the dispensed blood sample.

[0045] The blood sedimentation detection module 10 comprises a detection pipeline 101, an optical signal generation module 102, an optical signal collection module 103, a heating module 104, and a control module 105 connected with the optical signal generation module 102, the optical signal collection module 103, and the heating module 104. The control module 105 can control the opening or closing of other modules connected therewith.

[0046] The detection pipeline 101 is configured to provide a detection site for the blood sample, i.e., the detection pipeline 101 is configured to load the blood sample. The blood sample dispensed by the sample dispensing module 11 is dispensed into the detection pipeline 101, and the blood sample is detected in the detection pipeline 101.

[0047] In this embodiment, when detecting the blood sedimentation, the blood sample needs to be depolymerized in advance, i.e., using a syringe or other sample suction device to repeatedly suck and spit the blood sample, so that the blood sample moves back and forth in the detection pipeline 101, so as to achieve the purpose of dispersing and uniformizing the red blood cells in the detection pipeline 101, which is beneficial to make the test result of the subsequent blood sedimentation detection more real. After the red blood cells are uniformized, the syringe stops sucking and spitting the sample, the depolymerization process of the red blood cells is completed, and the red blood cells start to gradually aggregate and sediment, i.e., enter the blood sedimentation measurement stage, and the blood sedimentation detection of the blood sample can be performed.

[0048] The optical signal generation module 102 is configured to generate a light source, and the generated light source is configured to irradiate the blood sample dispensed into the detection pipeline 101. The device for generating the light source can be an LED lamp or other light-emitting device. During the light irradiation of the blood sample, the blood sample absorbs part of the light and scatters the unabsorbed light outward. The optical signal collection module 103 can collect the optical signal of the scattered light, and the collected optical signal is used for analysis and processing to obtain the erythrocyte sedimentation rate of the blood sample.

[0049] In this embodiment, the optical signal collection module 103 can be provided with a photoelectric conversion device for converting the optical signal into an electrical signal to realize the collection of the optical signal. The photoelectric conversion device can be a photodiode, a photoelectric sensor, or other device based on the principle of photoelectric effect to convert the optical signal into an electrical signal.

[0050] After the conversion of the electrical signal, in order to facilitate the transmission of the signal, the optical signal acquisition module 103 can further be provided with an analog-digital conversion device for converting the electrical signal into a digital signal.

[0051] In this embodiment, the sample analyzer can further be provided with a signal processing module 12, which can receive the collected electrical signal and analyze and process the electrical signal according to a preset processing algorithm and processing program, so as to obtain the erythrocyte sedimentation rate detection result of the blood sample.

[0052] The test of the erythrocyte sedimentation rate is affected by various factors, among which the temperature change has a greater impact on the test result of the erythrocyte sedimentation rate. Therefore, during the erythrocyte sedimentation rate detection process, the temperature of the blood sample needs to be strictly controlled to be within a certain temperature range. In this embodiment, the heating module 104 can be used to control the temperature of the blood sample in the detection pipeline 101 and heat the blood sample, so as to maintain the temperature of the blood sample within a preset temperature range.

[0053] The heating module 104 can be provided with a temperature sensor for sensing the temperature of the blood sample. When the temperature of the blood sample in the detection pipeline 101 is within the preset temperature range, the control module 105 controls the heating module 104 to be turned off to stop heating the blood sample. When the temperature of the blood sample in the detection pipeline 101 is lower than the preset temperature range, the control module 105 controls the heating module 104 to be turned on to start heating the blood sample, so as to raise the temperature of the blood sample to be within the preset temperature range.

[0054] By strictly controlling the temperature of the blood sample, the influence of the temperature on the erythrocyte sedimentation rate detection result can be reduced or even eliminated, and the accuracy of the detection result can be improved.

[0055] Since the heating module 104 will be turned on and turned off multiple times, and the heating module 104 is usually made of conductive materials such as metal, each time the heating module 104 is turned on or turned off, an electromagnetic signal that interferes with the optical signal acquisition will be generated, and the generated electromagnetic signal will last for a very short period of time, which will cause the collected optical signal to deviate, thereby affecting the subsequent signal processing process and the final erythrocyte sedimentation rate detection result.

[0056] As Figure 2As shown, curve a represents the curve of the electrical signal obtained by photoelectric conversion of the optical signal, and the collection of the optical signal is disturbed at the moment when the heating module 104 is switched from heating to non-heating (i.e. the heating module 104 is turned off) or from non-heating to heating (i.e. the heating module 104 is turned on). After photoelectric conversion, it can be seen that the disturbed electrical signal has a spike at the switching moment of turning on or turning off. If the electrical signal with the deviation is used for blood sedimentation analysis, the final detection result will be inaccurate.

[0057] Therefore, in this embodiment, to avoid the collection of the optical signal being disturbed, the control module 105 can control the optical signal collection module 103 to collect the optical signal of the light absorption or scattering of the blood sample outside the time period when the heating module 104 brings disturbance signals due to turning on or turning off. Since the heating module 104 does not perform the action of turning on or turning off, the collection of the optical signal will not be disturbed, and accurate optical signals can be collected. Within the time period when the heating module 104 brings disturbance signals due to turning on or turning off, the control module 105 can control the optical signal collection module 103 to suspend the collection of the optical signal, so as to skip the time period when electromagnetic interference is generated, so that the collection of the optical signal avoids this interference, and the finally collected optical signal does not exist the disturbed optical signal, which can be used for signal processing and analysis to obtain accurate detection results.

[0058] In this embodiment, the control module 105 is in signal connection with the optical signal generation module 102, the optical signal collection module 103 and the heating module 104, and controls the turning on or turning off of other modules in communication connection. Outside the time period when the heating module 104 brings disturbance signals due to turning on or turning off, the control module 105 controls the optical signal collection module 103 to collect the optical signal, and the collection of the optical signal can not be disturbed. Within the time period when the heating module 104 brings disturbance signals due to turning on or turning off, the control module 105 controls the optical signal collection module 103 to suspend the collection of the optical signal, so as to avoid collecting the disturbed optical signal. Through the linkage control of the optical signal collection module 103 and other modules, pure and undisturbed optical signals can be collected in the whole signal collection process, which is beneficial to the subsequent signal processing and analysis process.

[0059] In this embodiment, the control module 105 needs to control the collection operation of the optical signal collection module 103 according to the turning on or turning off of the heating module 104. To make the control of the optical signal collection more simple and efficient, the control module 105 can control the optical signal collection module 103 to have equal time intervals between every two adjacent collection time points.

[0060] As shown in FIG. 8, the control module 105 controls the optical signal collection module 103 to collect the optical signal at the first collection time point t1, and then controls the optical signal collection module 103 to collect the optical signal at the second collection time point t2. The time interval between the first collection time point t1 and the second collection time point t2 is equal to the time interval between the second collection time point t2 and the third collection time point t3, and so on. Figure 2As shown, the control light signal collection module 103 has a time interval of T between the collection time points 1 and 2, a time interval of T between the collection time points 2 and 3, and a time interval of T between the collection time points 3 and 4, and so on. That is, the light signal collection module 103 collects light signals again after T time, which makes the control of light signal collection follow a set rule, thereby making the control more simple and efficient.

[0061] In this embodiment, the switching of the heating module 104 on / off generates electromagnetic interference. To this end, the switching frequency of the heating module 104 can be reduced to reduce the impact. That is, the heating module 104 is stopped for a long period of time, and even if the temperature of the blood sample is lower than the preset temperature range, the heating module 104 does not need to be turned on. To ensure that the electrical signal for blood sedimentation analysis corresponds to the preset temperature range and ensures the accuracy of the blood sedimentation result, the electrical signal collected below the preset temperature range needs to be corrected to the electrical signal corresponding to the preset temperature range.

[0062] To achieve the above-mentioned purpose, the control module 105 can continue to control the light signal collection module 103 to collect light signals after the heating module 104 stops heating, and convert the collected light signals into electrical signals. Since the temperature of the blood sample decreases after the heating is stopped, the collected electrical signals must include electrical signals below the preset temperature range, which can be referred to as to-be-corrected electrical signals. Then, the control module 105 corrects the to-be-corrected electrical signals according to a preset correction algorithm, and corrects the to-be-corrected electrical signals to standard electrical signals corresponding to the preset temperature range.

[0063] Therefore, in the above-mentioned correction method, since the heating module 104 is not turned on for a short period of time after the heating is stopped, the switching frequency of the heating module 104 can be reduced, thereby reducing the generation of interference signals, and the accuracy of the blood sedimentation detection result can be ensured through the correction algorithm.

[0064] In this embodiment, there are multiple correction algorithms for correcting the to-be-corrected electrical signal into the standard electrical signal, and therefore, the control module 105 has multiple ways to correct the to-be-corrected electrical signal. In one way, the control module 105 is configured to construct a functional relationship between a temperature difference and a difference between electrical signal detection values, where the temperature difference is a difference between any temperature and a standard temperature within a preset temperature range, and the difference between electrical signal detection values is a detection value difference between an electrical signal collected by the optical signal collection module 103 at the any temperature and an electrical signal collected at the standard temperature. The functional relationship is constructed, i.e., the two variables of the temperature difference and the difference between electrical signal detection values are fitted. The fitting method can be an XGBoost algorithm, a linear regression algorithm, a logistic regression algorithm, a naive Bayes algorithm, a k-nearest neighbors (KNN) algorithm, a random forest algorithm, etc.

[0065] After the functional relationship between the temperature difference and the difference between electrical signal detection values is established, the control module 105 calculates a first difference between a temperature corresponding to the to-be-corrected electrical signal and the standard temperature, and substitutes the first difference into the functional relationship to calculate a second difference between a detection value of the to-be-corrected electrical signal and an electrical signal detection value corresponding to the standard temperature. Then, the second difference is added to the detection value of the to-be-corrected electrical signal to calculate a detection value of the standard electrical signal.

[0066] For example, the functional relationship between the temperature difference and the difference between electrical signal detection values can be expressed as: y=kx+b; where k and b are parameters of the functional relationship, variable x is the temperature difference, and variable y is the difference between electrical signal detection values.

[0067] Suppose the preset temperature range is set to [36℃, 38℃], and the standard temperature within the preset temperature range is set to 37℃, in order to fit the temperature difference and the difference between electrical signal detection values in the functional relationship to obtain the parameters of the functional relationship, the temperature difference between each any temperature and 37℃ can be calculated, and the difference between the electrical signal detection value corresponding to each any temperature and the electrical signal detection value corresponding to 37℃ can be calculated. The fitting algorithm is used to fit the temperature difference and the difference between electrical signal detection values, and the parameters of the functional relationship can be fitted.

[0068] After obtaining the parameters of the functional relationship, it can be used to correct the electrical signal to be corrected. For example, after acquiring the electrical signal to be corrected corresponding to 35℃, the electrical signal to be corrected is corrected by calculating the temperature difference between 35℃ and 37℃, and substituting this temperature difference into the functional relationship y=kx+b (where the values ​​of k and b are already determined), the difference between the detected value of the electrical signal to be corrected and the detected value of the electrical signal corresponding to 37℃ is calculated. Then, this difference is added to the detected value of the electrical signal to be corrected to calculate the detected value of the electrical signal corresponding to the preset temperature range, that is, the detected value of the standard electrical signal.

[0069] It should be noted that the functional relationships listed above are only illustrative examples. In actual operation, the functional relationship between the temperature difference and the difference of the electrical signal detection value can be expressed in any form, such as a quadratic function, a cubic function, etc., as long as it can express the functional relationship between the temperature difference and the difference of the electrical signal detection value. The specific functional expression is not limited.

[0070] In this embodiment, during multiple duration periods when interference signals are generated due to the heating module 104 being turned on or off, the control module 105 can control the optical signal acquisition module 103 to pause or collect optical signals. However, the optical signals collected during these multiple duration periods need to be processed to eliminate interference signals. Therefore, the optical signals collected during these multiple duration periods are photoelectrically converted by the optical signal acquisition module 103 into electrical signals, resulting in multiple interference-affected electrical signals corresponding to these multiple duration periods.

[0071] like Figure 2 As shown, the upward and downward peaks in curve a are multiple interference signals corresponding to multiple duration segments. The different directions of the peaks of the interference signals indicate that the detected values ​​of these multiple interference signals are either positive or negative. Therefore, the detected values ​​of these multiple interference signals can be integrated to make the integral value of the detected values ​​of these multiple interference signals zero, thereby eliminating the influence of these multiple interference signals on the erythrocyte sedimentation rate (ESR) test results.

[0072] Figure 3The schematic diagram of the electric signal collected by the light signal collection module 103 is shown in the figure. In the duration period when the heating module 104 is turned on (switched from "not heating" to "heating"), m upward disturbed electric signals are collected. In the duration period when the heating module 104 is turned off (switched from "heating" to "not heating"), m downward disturbed electric signals are also collected. In this way, in the plurality of duration periods, a plurality of disturbed electric signals pointing in different directions can be collected. The plurality of disturbed electric signals are integrated, so that the integral value of the detection value of the plurality of disturbed electric signals is zero, that is, Xc[0]+…+Xc[m]+…+Xc[n]+…+Xc[n+m]=0, thereby eliminating the influence of the disturbed electric signal on the blood sedimentation analysis process.

[0073] It can be understood that one of the reasons for the electromagnetic interference caused by the opening / closing of the heating module is that the heating module is made of metal material. The conductive property of metal provides a propagation path for electromagnetic interference. Therefore, in order to reduce electromagnetic interference, the heating module can also be made of a material with good thermal conductivity and non-conductive property, for example, the heating module is made of thermal conductive silicone, thermal conductive silicone grease and the like.

[0074] In the embodiment, in the plurality of duration periods, the control module 105 can control the light signal collection module 103 to collect the light signal at a collection frequency higher than the normal collection frequency. The normal collection frequency refers to the collection frequency of the light signal collection module 103 to the light signal outside the time period when the heating module 104 brings interference signals. For example, the normal collection frequency of the light signal collection module 103 to the light signal outside the time period when the heating module 104 brings interference signals is 100 Hz. In the plurality of duration periods, the control module 105 can control the light signal collection module 103 to collect the light signal at a collection frequency of 10 KHz. Through high-frequency collection, more disturbed electric signals can be collected, which is conducive to eliminating the disturbed electric signals by integration and making the integral calculation of the disturbed electric signals more accurate.

[0075] In the embodiment of the present application, the sample analyzer can not need to collect the light signal according to the opening or closing of the heating module, but can keep collecting the light signal throughout the blood sedimentation measurement stage, that is, the light signal is collected even in the duration period when the interference signal is generated. Please refer to Figure 4 Another embodiment of the sample analyzer in the embodiment of the present application includes:

[0076] The blood sedimentation detection module 40 and the sampling distribution module 41;

[0077] The sampling distribution module 41 is used to collect the blood sample and distribute the blood sample to the blood sedimentation detection module.

[0078] The blood sedimentation detection module 40 comprises a detection pipeline 401, a light signal generation module 402, a light signal collection module 403, a heating module 404 and a signal processing module 405.

[0079] The detection pipeline 401 is used to provide a detection site for the blood sample.

[0080] The light signal generation device 402 generates a light source and is used to irradiate the blood sample distributed in the detection pipeline 401 with light.

[0081] The heating module 404 heats the blood sample in the detection pipeline 401 so that the blood in the detection pipeline 401 is maintained within a preset temperature range.

[0082] The functions performed by each module of the sample analyzer in the embodiment are similar to those described in the foregoing Figure 1 embodiments and will not be described here again.

[0083] In the embodiment, the light signal collection module 403 collects the light signal of the absorption or scattering of light by the blood sample distributed in the detection pipeline 101 at a first frequency, i.e., the light signal collection module 403 does not need to collect the light signal according to the opening or closing of the heating module 404, but maintains the collection of the light signal throughout the blood sedimentation measurement stage, i.e., the light signal is collected even during the duration of the interference signal generated by the opening / closing of the heating module 404.

[0084] Since the light signal collected during the duration will be interfered by the opening or closing of the heating module 404, the interference needs to be eliminated, therefore, after the light signal is collected, the signal processing module 405 can receive the collected light signal and convert the light signal into an electrical signal, integrate the electrical signal to eliminate the interference signal, and calculate the erythrocyte sedimentation rate of the blood sample according to the electrical signal after the elimination of the interference.

[0085] The process of integrating the electrical signal is similar to the process of integrating the interfered electrical signal in the foregoing Figure 1 embodiments and will not be described here again.

[0086] In a preferred embodiment, the first frequency is greater than or equal to 9 kHz.

[0087] In order to save the cost of the instrument and save the occupied space of the instrument, the signal processing module 405 can be integrated with the light signal collection module 403, and the functions performed by the two can be integrated in the same module, which can greatly save the occupied space of the sample analyzer and reduce the volume of the sample analyzer.

[0088] The sample analyzer of the embodiment of the application is described above, and the functions of the foregoing Figure 1Based on the embodiments shown, the signal acquisition method implemented by the sample analyzer is described in further detail Figure 1 The signal acquisition method implemented by the sample analyzer of the embodiments shown is described in further detail Figure 5 The signal acquisition method of the embodiments of the present application includes the following steps:

[0089] 501. Distribute the blood sample to be tested to the detection pipeline;

[0090] In the embodiments, the sample analyzer can suck a certain amount of blood sample from the sample pool through the sampling distribution module, and distribute the sucked blood sample to the detection pipeline of the blood sedimentation detection module by the sampling distribution module.

[0091] 502. Heat the blood sample in the detection pipeline by the heating module;

[0092] The sample analyzer heats the blood sample in the detection pipeline by the heating module, which can be achieved by setting a temperature sensor to sense the temperature of the blood sample in the detection pipeline. When the temperature of the blood in the detection pipeline is within the preset temperature range, the heating module is turned off to stop heating the blood. When the temperature of the blood in the detection pipeline is below the preset temperature range, the heating module is turned on to start heating the blood, so that the blood in the detection pipeline is maintained within the preset temperature range.

[0093] 503. Control the light signal acquisition module to acquire the light signal of the absorption or scattering of light by the blood sample distributed to the detection pipeline;

[0094] In the embodiments, to avoid interference with the acquisition of the light signal, the light signal acquisition module is controlled to acquire the light signal of the absorption or scattering of light by the blood sample outside the time period when the heating module is turned on or turned off to cause interference signals. Since the heating module does not perform the action of turning on or turning off, the acquisition of the light signal will not be disturbed, and accurate light signals can be acquired. Within the time period when the heating module is turned on or turned off to cause interference signals, the light signal acquisition module is controlled to suspend the acquisition of the light signal, skipping the time period when electromagnetic interference occurs, so that the acquisition of the light signal avoids this period of interference, and the light signal acquired ultimately does not exist interference, which can be used for signal processing and analysis to obtain accurate blood sedimentation detection results.

[0095] In the embodiments, to make the control of the light signal acquisition more simple and efficient, the time interval between each two adjacent acquisition time points of the light signal acquisition module can be controlled to be equal.

[0096] 504. Convert the light signal into an electrical signal;

[0097] In the embodiments, the photoelectric conversion device in the light signal acquisition module can convert the light signal into an electrical signal to achieve the acquisition of the light signal.

[0098] 505. correcting the to-be-corrected electrical signal according to a preset correction algorithm, so as to correct the to-be-corrected electrical signal into a standard electrical signal corresponding to the preset temperature range;

[0099] In this embodiment, the switching of the heating module on / off will generate electromagnetic interference. Therefore, the switching frequency of the heating module can be reduced to reduce the influence, that is, the heating is stopped for a long period of time, and the heating module does not need to be turned on even if the temperature of the blood sample is lower than the preset temperature range. In order to ensure that the electrical signal for blood sedimentation analysis corresponds to the preset temperature range and ensure the accuracy of the blood sedimentation result, the electrical signal collected below the preset temperature range needs to be corrected to the electrical signal corresponding to the preset temperature range.

[0100] Therefore, after the heating module stops heating, the light signal acquisition module continues to acquire the light signal, and the acquired light signal is converted into an electrical signal. Since the temperature of the blood sample will decrease after the heating is stopped, there must be an electrical signal below the preset temperature range in the acquired electrical signal, which can be referred to as a to-be-corrected electrical signal. Then, the to-be-corrected electrical signal is corrected according to a preset correction algorithm, so as to correct the to-be-corrected electrical signal into a standard electrical signal corresponding to the preset temperature range.

[0101] In this embodiment, there are various ways to correct the to-be-corrected electrical signal. In one way, a function relationship between a temperature difference and a difference between electrical signal detection values is constructed, the temperature difference is the difference between any temperature and a standard temperature in the preset temperature range, and the difference between electrical signal detection values is the difference between the detection values of the electrical signal acquired by the light signal acquisition module at the any temperature and the electrical signal acquired at the standard temperature. The function relationship is constructed, that is, the temperature difference and the difference between electrical signal detection values are fitted. The specific fitting method can use fitting algorithms such as XGBoost algorithm, linear regression algorithm, logistic regression algorithm, naive Bayes algorithm, k-nearest neighbor algorithm (K-Nearest Neighbors, KNN), random forest algorithm (RandomForest), etc.

[0102] After the function relationship between the temperature difference and the difference between electrical signal detection values is established, a first difference value between the temperature corresponding to the to-be-corrected electrical signal and the standard temperature is calculated, and the first difference value is substituted into the function relationship to calculate a second difference value between the detection value of the to-be-corrected electrical signal and the electrical signal detection value corresponding to the standard temperature. Then, the second difference value and the detection value of the to-be-corrected electrical signal are added to calculate the detection value of the standard electrical signal.

[0103] In the embodiment, the light signal collecting module can be controlled to suspend collecting the light signal in the plurality of time periods during which the heating module is turned on or turned off to generate the interference signal, or the light signal collecting module can be controlled to collect the light signal in the plurality of time periods during which the heating module is turned on or turned off to generate the interference signal, but the light signal collected in the plurality of time periods needs to be processed to eliminate the interference signal. Therefore, the light signal collected in the plurality of time periods is photoelectrically converted by the light signal collecting module into an electrical signal to obtain a plurality of disturbed electrical signals corresponding to the plurality of time periods.

[0104] Then, the detection values of the plurality of disturbed electrical signals are integrated so that the integral values of the detection values of the plurality of disturbed electrical signals are zero, thereby eliminating the influence of the plurality of disturbed electrical signals on the sedimentation detection result.

[0105] In the embodiment, the light signal collecting module can be controlled to collect the light signal at a higher frequency than the normal collecting frequency in the plurality of time periods, where the normal collecting frequency refers to the frequency at which the light signal collecting module collects the light signal outside the time period during which the heating module is turned on or turned off to generate the interference signal. For example, the normal collecting frequency of the light signal collecting module for collecting the light signal outside the time period during which the heating module is turned on or turned off to generate the interference signal is 100 Hz, and the control module can control the light signal collecting module to collect the light signal at a frequency of 10 KHz in the plurality of time periods. By collecting at a high frequency, more disturbed electrical signals can be collected, which is conducive to eliminating the disturbed electrical signals by integration and makes the integral calculation of the disturbed electrical signals more accurate.

[0106] In the embodiment, the light signal collecting module collects the light signal outside the time period during which the heating module is turned on or turned off to generate the interference signal, and the collection of the light signal can not be disturbed; and the light signal collecting module suspends collecting the light signal in the time period during which the heating module is turned on or turned off to generate the interference signal, thereby avoiding collecting the disturbed light signal. By controlling the light signal collecting module and other modules in linkage, pure and undisturbed light signals can be collected in the entire signal collection process, which is conducive to subsequent signal processing and analysis.

[0107] The signal collection method implemented by the sample analyzer according to the embodiment shown in Figure 4 will be described in detail below on the basis of the foregoing Figure 4 The signal collection method implemented by the sample analyzer according to the embodiment shown in Figure 6 , one embodiment of the signal collection method in the embodiment of the application includes the following steps:

[0108] 601. Distribute the blood sample to be detected to the detection pipeline;

[0109] In this embodiment, the sample analyzer can suck a certain amount of blood sample from the sample pool through the sampling and dispensing module, and dispense the sucked blood sample into the detection pipeline of the blood sedimentation detection module by the sampling and dispensing module.

[0110] 602、heating the blood sample in the detection pipeline by the heating module to maintain the blood in the detection pipeline within a preset temperature range;

[0111] In this embodiment, the sample analyzer heats the blood sample in the detection pipeline by the heating module to maintain the temperature of the blood sample in the detection pipeline within a preset temperature range.

[0112] 603、controlling the light signal acquisition module to acquire, at a first frequency, a light signal of light absorbed or scattered by the blood sample dispensed into the detection pipeline;

[0113] In this embodiment, the light signal acquisition module does not need to acquire the light signal according to the opening or closing of the heating module, but maintains the acquisition of the light signal throughout the blood sedimentation measurement stage, that is, the light signal is still acquired during the duration of the interference signal generated by the opening / closing of the heating module.

[0114] In a preferred embodiment, the first frequency is greater than or equal to 9 kHz.

[0115] 604、converting the acquired light signal into an electrical signal, integrating the electrical signal to eliminate the interference signal, and obtaining the erythrocyte sedimentation rate of the blood sample according to the electrical signal after eliminating the interference;

[0116] Since the light signal acquired during the duration will be interfered by the opening or closing of the heating module, the interference needs to be eliminated, therefore, after the light signal is acquired, the light signal is converted into an electrical signal, the electrical signal is integrated to eliminate the interference signal, and the erythrocyte sedimentation rate of the blood sample is calculated according to the electrical signal after eliminating the interference.

[0117] In which, the process of integrating the electrical signal is similar to the process of integrating the interfered electrical signal in the foregoing Figure 5 The embodiment shown in the foregoing

[0118] The embodiments of the present application also provide a computer storage medium, wherein one embodiment includes: the computer storage medium stores instructions, and the instructions are executed on a computer to make the computer execute the operations of the sample analyzer in the foregoing Figures 5 to 6 The embodiments shown in the foregoing

[0119] Those skilled in the art will clearly understand that, for the sake of convenience and brevity, the specific working processes of the systems, devices, and units described above can be referred to the corresponding processes in the foregoing method embodiments, and will not be repeated here.

[0120] In the several embodiments provided in this application, it should be understood that the disclosed systems, apparatuses, and methods can be implemented in other ways. For example, the apparatus embodiments described above are merely illustrative; for instance, the division of units is only a logical functional division, and in actual implementation, there may be other division methods. For example, multiple units or components may be combined or integrated into another system, or some features may be ignored or not executed. Furthermore, the coupling or direct coupling or communication connection shown or discussed may be an indirect coupling or communication connection between apparatuses or units through some interfaces, and may be electrical, mechanical, or other forms.

[0121] The units described as separate components may or may not be physically separate. The components shown as units may or may not be physical units; that is, they may be located in one place or distributed across multiple network units. Some or all of the units can be selected to achieve the purpose of this embodiment according to actual needs.

[0122] Furthermore, the functional units in the various embodiments of this application can be integrated into one processing unit, or each unit can exist physically separately, or two or more units can be integrated into one unit. The integrated unit can be implemented in hardware or as a software functional unit.

[0123] If the integrated unit is implemented as a software functional unit and sold or used as an independent product, it can be stored in a computer-readable storage medium. Based on this understanding, the technical solution of this application, in essence, or the part that contributes to the prior art, or all or part of the technical solution, can be embodied in the form of a software product. This computer software product is stored in a storage medium and includes several instructions to cause a computer device (which may be a personal computer, server, or network device, etc.) to execute all or part of the steps of the methods described in the various embodiments of this application. The aforementioned storage medium includes various media capable of storing program code, such as USB flash drives, portable hard drives, read-only memory (ROM), random access memory (RAM), magnetic disks, or optical disks.

Claims

1. A sample analyzer, characterized in that, Includes an erythrocyte sedimentation rate (ESR) detection module and a sample distribution module; The sampling and distribution module is used to collect blood samples and distribute the blood samples to the erythrocyte sedimentation rate (ESR) detection module; The erythrocyte sedimentation rate (ESR) detection module includes a detection pipeline, a light signal generation module, a light signal acquisition module, a heating module, and a control module that is signal-connected to the light signal generation module, the light signal acquisition module, and the heating module. The detection tubing is used to provide a testing site for the blood sample; The optical signal generating module generates a light source and is used to irradiate the blood sample distributed to the detection pipeline. The optical signal acquisition module acquires the light signal absorbed or scattered by the blood sample distributed to the detection pipeline to detect the erythrocyte sedimentation rate of the blood sample; The heating module heats the blood sample in the detection tube to maintain the blood in the detection tube within a preset temperature range. When the temperature of the blood in the detection tube is within the preset temperature range, the control module controls the heating module to turn off to stop heating the blood. When the temperature of the blood in the detection tube is lower than the preset temperature range, the control module controls the heating module to turn on to start heating the blood. During the period when the heating module is turned on or off, causing interference signals, the control module controls the optical signal acquisition module to pause the acquisition of optical signals. Outside the period when the heating module is turned on or off, causing interference signals, the control module controls the optical signal acquisition module to acquire the light signals absorbed or scattered by the blood sample distributed to the detection pipeline to detect the erythrocyte sedimentation rate of the blood sample.

2. The sample analyzer according to claim 1, characterized in that, The control module is used to ensure that the time interval between any two adjacent acquisition time points of the optical signal acquisition module is equal.

3. The sample analyzer according to claim 1, characterized in that, The control module is used to control the optical signal acquisition module to acquire the optical signal after the heating module stops heating; The optical signal acquisition module is also used to convert the optical signal into an electrical signal; The control module is used to correct the electrical signal to be corrected according to a preset correction algorithm, and to correct the electrical signal to be corrected to a standard electrical signal corresponding to the preset temperature range. The electrical signal to be corrected is an electrical signal obtained by converting the optical signal collected below the preset temperature range.

4. The sample analyzer according to claim 3, characterized in that, The control module is used to construct a functional relationship between the temperature difference and the difference between the electrical signal detection value. The temperature difference is the difference between any temperature and a standard temperature within the preset temperature range. The difference between the electrical signal detection values ​​is the difference between the detection values ​​of the electrical signal acquired by the optical signal acquisition module at any temperature and the electrical signal acquired at the standard temperature. The control module is used to calculate a first difference between the temperature corresponding to the electrical signal to be corrected and the standard temperature, and substitute the first difference into the function relationship to calculate a second difference between the detection value of the electrical signal to be corrected and the detection value of the electrical signal corresponding to the standard temperature. The control module is used to add the second difference to the detection value of the electrical signal to be corrected, and calculate the detection value of the standard electrical signal.

5. A sample analyzer, characterized in that, Includes an erythrocyte sedimentation rate (ESR) detection module and a sample distribution module; The sampling and distribution module is used to collect blood samples and distribute the blood samples to the erythrocyte sedimentation rate (ESR) detection module; The erythrocyte sedimentation rate (ESR) detection module includes a detection pipeline, an optical signal generation module, an optical signal acquisition module, a heating module, and a signal processing module. The detection tubing is used to provide a testing site for the blood sample; The optical signal generating module generates a light source and is used to irradiate the blood sample distributed to the detection pipeline. The heating module heats the blood sample in the detection pipeline to maintain the blood in the detection pipeline within a preset temperature range. The optical signal acquisition module acquires the optical signal of the blood sample absorbed or scattered by the blood sample distributed to the detection pipeline at a first frequency; The signal processing module receives the optical signals collected by the optical signal acquisition module during multiple duration periods when the heating module is turned on or off and generates interference signals, and converts the optical signals into electrical signals to obtain multiple interference electrical signals corresponding to the multiple duration periods; the multiple interference electrical signals corresponding to the multiple duration periods are integrated to eliminate the interference signals, and the erythrocyte sedimentation rate of the blood sample is obtained based on the interference-free electrical signals.

6. The sample analyzer according to claim 5, characterized in that, The first frequency is greater than or equal to 9 kHz.

7. The sample analyzer according to claim 5 or 6, characterized in that, The signal processing module and the optical signal acquisition module are integrated into one unit.

8. The sample analyzer according to claim 5, characterized in that, The step of integrating the multiple interfered electrical signals corresponding to the multiple duration segments to eliminate the interference signals includes: The detected values ​​of the plurality of interfered electrical signals are integrated so that the integral value of the detected values ​​of the plurality of interfered electrical signals is zero.

9. The sample analyzer according to claim 8, characterized in that, During the multiple duration periods, the signal processing module controls the optical signal acquisition module to acquire the optical signal at a higher acquisition frequency than the normal acquisition frequency. The normal acquisition frequency is the acquisition frequency of the optical signal by the optical signal acquisition module outside the time period when the heating module is turned on or off, causing interference signals.

10. A signal acquisition method, characterized in that, The signal acquisition method is applied to the sample analyzer according to any one of claims 1 to 4, and the method includes: Dispense the blood sample to be tested into the testing tubing; The blood sample in the detection tube is heated by the heating module to keep the blood in the detection tube within a preset temperature range. When the temperature of the blood in the detection tube is within the preset temperature range, the heating module is turned off to stop heating the blood. When the temperature of the blood in the detection tube is lower than the preset temperature range, the heating module is turned on to start heating the blood. During the period when the heating module is turned on or off, causing interference signals, the control module controls the optical signal acquisition module to pause the acquisition of optical signals. Outside the period when the heating module is turned on or off, causing interference signals, the control module controls the optical signal acquisition module to acquire the light signals absorbed or scattered by the blood sample distributed to the detection pipeline to detect the erythrocyte sedimentation rate of the blood sample.

11. The signal acquisition method according to claim 10, characterized in that, The control of the optical signal acquisition module to acquire the light signal absorbed or scattered by the blood sample distributed to the detection pipeline includes: The time interval between any two adjacent acquisition points is kept equal by the optical signal acquisition module.

12. The signal acquisition method according to claim 10, characterized in that, The control of the optical signal acquisition module to acquire the light signal absorbed or scattered by the blood sample distributed to the detection pipeline includes: After the heating module stops heating, the optical signal acquisition module is controlled to acquire the optical signal; The method further includes: The optical signal is converted into an electrical signal; The electrical signal to be corrected is corrected according to a preset correction algorithm, and the electrical signal to be corrected is corrected to a standard electrical signal corresponding to the preset temperature range. The electrical signal to be corrected is the electrical signal obtained by converting the optical signal collected below the preset temperature range.

13. The signal acquisition method according to claim 12, characterized in that, The step of correcting the electrical signal to be corrected according to the preset correction algorithm includes: A functional relationship is established between the temperature difference and the difference between the detected electrical signal values. The temperature difference is the difference between any temperature and a standard temperature within the preset temperature range. The difference between the detected electrical signal values ​​is the difference between the detected electrical signal acquired by the optical signal acquisition module at any temperature and the detected electrical signal acquired at the standard temperature. Calculate the first difference between the temperature corresponding to the electrical signal to be corrected and the standard temperature, substitute the first difference into the functional relationship, and calculate the second difference between the detected value of the electrical signal to be corrected and the detected value of the electrical signal corresponding to the standard temperature. The second difference is added to the detection value of the electrical signal to be corrected to calculate the detection value of the standard electrical signal.

14. A signal acquisition method, characterized in that, The signal acquisition method is applied to the sample analyzer according to any one of claims 5 to 9, and the method includes: Dispense the blood sample to be tested into the testing tubing; The blood sample in the detection tubing is heated by a heating module to maintain the blood in the detection tubing within a preset temperature range; The optical signal acquisition module is controlled to acquire the light signal absorbed or scattered by the blood sample distributed to the detection pipeline at a first frequency; The optical signals collected during multiple duration periods when interference signals are generated due to the heating module being turned on or off are converted into electrical signals to obtain multiple interference electrical signals corresponding to the multiple duration periods; the multiple interference electrical signals corresponding to the multiple duration periods are integrated to eliminate the interference signals, and the erythrocyte sedimentation rate of the blood sample is obtained based on the interference-free electrical signals.

15. The signal acquisition method according to claim 14, characterized in that, The first frequency is greater than or equal to 9 kHz.

16. The signal acquisition method according to claim 14, characterized in that, The step of integrating the multiple interfered electrical signals corresponding to the multiple duration segments to eliminate the interference signals includes: The detected values ​​of the plurality of interfered electrical signals are integrated so that the integral value of the detected values ​​of the plurality of interfered electrical signals is zero.

17. The signal acquisition method according to claim 16, characterized in that, During the multiple duration periods, the optical signal acquisition module is controlled to acquire the optical signal at a frequency higher than the normal acquisition frequency. The normal acquisition frequency is the frequency at which the optical signal acquisition module acquires the optical signal outside the time period when the heating module is turned on or off, causing interference signals.

18. A computer storage medium, characterized in that, The computer storage medium stores instructions that, when executed on the computer, cause the computer to perform the method as described in any one of claims 10 to 17.

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