Micro-droplet chip gas-liquid driving device

Abstract

The present invention provides a micro-droplet chip gas-liquid driving device, including an oil supply component, an air supply source component, and an oil injection needle. The air supply source component includes a floating oil-gas cavity and a first separated oil-gas cavity. The pressure airflow output by the air supply source component can be controlled to enter the aforementioned two cavities respectively. The oil injection needle has a first floating oil needle hole and a first separated oil needle hole. There is a first pipeline between the first floating oil needle hole and the floating oil-gas cavity. There is a second pipeline between the first separated oil needle hole and the first separated oil-gas cavity. The first pipeline and the second pipeline are both provided with oil storage components. The oil supply component supplies oil to the oil storage component. The oil in the oil storage components on the first pipeline and the second pipeline can be transported to the first floating oil needle hole and the first separated oil needle hole respectively under the action of the two air cavities. The present invention uses gas to push the liquid so that the liquid circuit is in a constant pressure state during droplet detection. The system has a fast dynamic response and good constant pressure stability, which can better meet the droplet detection requirements.

Description

Technical Field

[0001] The invention belongs to the technical field of digital PCR analyzers, and in particular relates to a micro-droplet chip gas-liquid driving device. Background Art

[0002] Digital PCR is the latest quantitative technology. It is based on the single-molecule PCR method to count nucleic acid quantification, which is an absolute quantitative method. It mainly uses microfluidics or dropletization methods to disperse a large amount of diluted nucleic acid solution into the microreactor or droplet of the chip, and the number of nucleic acid templates in each reactor is less than or equal to 1. In this way, after the PCR cycle, the microdroplets are irradiated with light of a specific wavelength. The reactor with a nucleic acid molecule template will give a specific fluorescence signal, and the reactor without a template will not have a specific fluorescence signal. According to the relative proportion and the volume of the reactor, the nucleic acid concentration of the original solution can be calculated. In the microdroplet chip analyzer, when the sample is detected, the sample droplets in the chip reservoir need to be driven into the floating oil channel through the floating oil, separated by the separator oil, and the adjacent droplets are separated, so that a certain distance is maintained between the droplet signals, which is convenient for signal analysis. When detecting droplets, there are strict requirements on the amount of floating oil and separator oil, the flow rate of droplets, and the speed ratio of floating oil to separator oil. The traditional detection oil driving method is to directly supply oil at a constant speed through a plunger pump, which can easily cause unstable pressure in the liquid system during the detection process, and droplets are prone to adhesion, affecting the signal analysis effect. Summary of the invention

[0003] Therefore, the technical problem to be solved by the present invention is to provide a micro-droplet chip gas-liquid driving device, which uses gas to push liquid to make the liquid circuit in a constant pressure state during droplet detection. The system has fast dynamic response and good constant pressure stability, which can better meet the droplet detection needs.

[0004] In order to solve the above problems, the present invention provides a micro-droplet chip gas-liquid driving device, including an oil supply component, an air supply source component, and an oil injection needle. The air supply source component includes a floating oil and gas cavity and a first separated oil and gas cavity. The pressurized airflow output by the air supply source component can be controlled to enter the floating oil and gas cavity and the first separated oil and gas cavity respectively. The oil injection needle has a first floating oil needle hole and a first separated oil needle hole. A first pipeline is provided between the first floating oil needle hole and the floating oil and gas cavity, and a second pipeline is provided between the first separated oil needle hole and the first separated oil and gas cavity. Oil storage components are provided on the first pipeline and the second pipeline. The oil supply component can be controlled to supply oil to the oil storage component. The oil in the oil storage component on the first pipeline can be transported to the first floating oil needle hole under the action of the floating oil and gas cavity, and the oil in the oil storage component on the second pipeline can be transported to the first separated oil needle hole under the action of the first separated oil and gas cavity.

[0005] In some embodiments, the oil storage assembly includes a vertically arranged oil storage pipe, a first electromagnetic three-way valve is provided at the upper end of the oil storage pipe, and a second electromagnetic three-way valve is provided at the lower end of the oil storage pipe, wherein the first electromagnetic three-way valve is configured to connect the floating oil gas cavity or the first separation oil gas cavity with the corresponding oil storage pipe when the air supply source assembly is needed to push the oil in the oil storage pipe, and to connect the oil storage pipe with the waste liquid tank when the oil supply assembly is needed to supply oil into the oil storage pipe, and the second electromagnetic three-way valve is configured to connect the oil storage pipe with the first floating oil needle hole or the first separation oil needle hole when the air supply source assembly is needed to push the oil in the oil storage pipe, and to connect the oil storage pipe with the oil supply assembly when the oil supply assembly is needed to supply oil into the oil storage pipe.

[0006] In some embodiments, an upper bubble sensor is provided between the upper end of the oil storage pipe and the first electromagnetic three-way valve, and a lower bubble sensor is provided between the lower end of the oil storage pipe and the second electromagnetic three-way valve.

[0007] In some embodiments, the air supply source assembly also includes a main air chamber, which is controllably connected to the floating oil and air chamber through a first solenoid valve, and the main air chamber is controllably connected to the first separated oil and air chamber through a second solenoid valve. The air supply source assembly also includes an air pump and a filter connected between the air pump and the main air chamber.

[0008] In some embodiments, the main air chamber, the floating oil-air chamber and the first separated oil-air chamber are respectively provided with a pressure sensor and a pressure relief valve.

[0009] In some embodiments, the oil outlet of the oil supply assembly is controllably connected to a bus, and the bus is also connected to the first pipeline and the second pipeline.

[0010] In some embodiments, the oil supply assembly includes an oil bottle and an oil pump, and the oil pump can pump the oil in the oil bottle to the bus and inject the oil into the oil storage pipe via the bus.

[0011] In some embodiments, a third solenoid valve is provided between the oil bottle and the oil pump.

[0012] In some embodiments, a fourth solenoid valve is provided on the pipeline between the second solenoid three-way valve and the first floating oil needle hole or the first separation oil needle hole.

[0013] In some embodiments, the oil injection needle has a second floating oil needle hole and a second separation oil needle hole, the air supply source assembly also includes a second separation oil and gas cavity controllably connected to the main air cavity, a third pipeline is provided between the second separation oil needle hole and the second separation oil and gas cavity, a fourth pipeline is provided between the second floating oil needle hole and the floating oil and gas cavity, and the oil storage assembly is provided on both the third pipeline and the fourth pipeline.

[0014] The present invention provides a micro-droplet chip gas-liquid driving device, which first transports the oil and stores it in the oil storage component through the oil supply component, and then controls the gas supply source component to push the oil into the corresponding channel of the micro-droplet chip through the pressure gas stored in the floating oil and gas cavity and the first separated oil and gas cavity when oil supply is needed. The plunger pump in the prior art is no longer used for constant speed oil supply, and the liquid circuit can be in a constant pressure state during droplet detection. The system has fast dynamic response and good constant pressure stability, and can better meet the droplet detection needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 Schematic diagram of the principle of the gas-liquid driving device of the micro-droplet chip according to an embodiment of the present invention.

[0016] The reference numerals are:

[0017] 11. Upward oil and gas chamber; 12. First separated oil and gas chamber; 13. Main air chamber; 131. First solenoid valve; 132. Second solenoid valve; 133. Fifth solenoid valve; 14. Air pump; 15. Filter; 161. Pressure sensor; 162. Pressure relief valve; 17. Second separated oil and gas chamber; 21. Oil storage pipe; 22. First solenoid three-way valve; 23. Second solenoid three-way valve; 24. Upper bubble sensor; 25. Lower bubble sensor; 3. Waste liquid tank; 4. Bus; 51. Oil bottle; 52. Oil pump; 53. Third solenoid valve; 6. Fourth solenoid valve; 100. Oil injection needle; 101. First upward oil needle hole; 102. First separated oil needle hole; 103. Second upward oil needle hole; 104. Second separated oil needle hole; 200. Micro droplet chip. DETAILED DESCRIPTION

[0018] See also Figure 1 As shown, according to an embodiment of the present invention, there is provided a micro-droplet chip gas-liquid driving device, comprising an oil supply component, an air supply source component, and an oil injection needle 100, wherein the air supply source component comprises an upward oil gas cavity 11 and a first separated oil gas cavity 12, and the pressure air flow output by the air supply source component can be controlled to enter the upward oil gas cavity 11 and the first separated oil gas cavity 12 respectively, and the oil injection needle 100 has a first upward oil needle hole 101 and a first separated oil needle hole 102, and a first pipeline is provided between the first upward oil needle hole 101 and the upward oil gas cavity 11, There is a second pipeline between the first oil separation needle hole 102 and the first oil separation gas cavity 12. The first pipeline and the second pipeline are both provided with oil storage components. The oil supply component can be controlled to supply oil to the oil storage component. The oil in the oil storage component on the first pipeline can be transported to the first floating oil needle hole 101 under the action of the floating oil gas cavity 11, and the oil in the oil storage component on the second pipeline can be transported to the first separation oil needle hole 102 under the action of the first separation oil gas cavity 12. In this technical solution, the oil is first transported and stored in the oil storage component through the oil supply component, and then when oil supply is needed, the gas supply source component is controlled to push the oil into the corresponding channel of the micro-droplet chip 200 through the pressure gas stored in the floating oil gas cavity 11 and the first separation oil gas cavity 12, respectively. The plunger pump in the prior art is no longer used to supply oil at a constant speed, and the liquid circuit can be in a constant pressure state during droplet detection. The system has a fast dynamic response and good constant pressure stability, which can better meet the droplet detection requirements.

[0019] As a specific implementation of the oil storage component, preferably, the oil storage component includes a vertically arranged oil storage pipe 21, a first electromagnetic three-way valve 22 is arranged at the upper end of the oil storage pipe 21, and a second electromagnetic three-way valve 23 is arranged at the lower end of the oil storage pipe 21, wherein the first electromagnetic three-way valve 22 is configured to connect the floating oil gas cavity 11 or the first separation oil gas cavity 12 with the corresponding oil storage pipe 21 when the air supply source component is needed to push the oil in the oil storage pipe 21, and to connect the oil storage pipe 21 with the waste liquid tank 3 when the oil supply component is needed to supply oil into the oil storage pipe 21, and the second electromagnetic three-way valve 23 is configured to connect the oil storage pipe 21 with the first floating oil needle hole 101 or the first separation oil needle hole 102 when the air supply source component is needed to push the oil in the oil storage pipe 21, and to connect the oil storage pipe 21 with the oil supply component when the oil supply component is needed to supply oil into the oil storage pipe 21.

[0020] In some embodiments, an upper bubble sensor 24 is provided between the upper end of the oil storage pipe 21 and the first electromagnetic three-way valve 22, and a lower bubble sensor 25 is provided between the lower end of the oil storage pipe 21 and the second electromagnetic three-way valve 23. The upper bubble sensor 24 and the lower bubble sensor 25 are respectively used to detect the oil height in the oil storage pipe 21, so as to control the operation of the corresponding air supply source component and the oil supply component according to the real-time height of the oil.

[0021] The air supply source assembly also includes a main air cavity 13, which is controllably connected to the floating oil and gas cavity 11 through a first solenoid valve 131, and is controllably connected to the first separation oil and gas cavity 12 through a second solenoid valve 132. The air supply source assembly also includes an air pump 14 and a filter 15 connected between the air pump 14 and the main air cavity 13. In this technical solution, the pressurized airflow delivered by the air pump 14 is buffered and stored in the main air cavity 13 to a preset pressure, and then the pressurized gas in the floating oil and gas cavity 11 and the first separation oil and gas cavity 12 can be replenished in real time, so that the pressure of the air-propelled oil is more constant.

[0022] The main air chamber 13, the floating oil and gas chamber 11 and the first separated oil and gas chamber 12 are respectively provided with a pressure sensor 161 and a pressure relief valve 162, which can effectively ensure the constancy of the pressure airflow in the main air chamber 13, the floating oil and gas chamber 11 and the first separated oil and gas chamber 12. The oil outlet of the oil supply component is controllably connected to the bus 4, and the bus 4 is also connected to the first pipeline and the second pipeline, which can simplify the design of the oil supply pipeline of the device. Specifically, the oil supply component includes an oil bottle 51 and an oil pump 52, and the oil pump 52 can pump the oil in the oil bottle 51 to the bus 4 and inject it into the oil storage pipe 21 through the bus 4.

[0023] A third electromagnetic valve 53 is provided between the oil bottle 51 and the oil pump 52, which is controlled to be connected when the oil in the oil storage pipe 21 is lower than the preset oil level. A fourth electromagnetic valve 6 is provided on the pipeline between the second electromagnetic three-way valve 23 and the first floating oil needle hole 101 or the first separation oil needle hole 102, which is controlled to be disconnected when the oil is injected into the oil storage pipe 21, and is controlled to be connected during the process of air pushing the oil.

[0024] In some embodiments, the oil injection needle 100 has a second floating oil needle hole 103 and a second separation oil needle hole 104, the air supply source assembly also includes a second separation oil and gas chamber 17 controllably connected to the main air chamber 13, the second separation oil and gas chamber 17 and the main air chamber 13 are controllably connected through the fifth solenoid valve 133, a third pipeline is provided between the second separation oil needle hole 104 and the second separation oil and gas chamber 17, a fourth pipeline is provided between the second floating oil needle hole 103 and the floating oil and gas chamber 11, and the third pipeline and the fourth pipeline are both provided with the oil storage assembly. It can be understood that in some embodiments, more groups of floating oil needle holes and separation oil needle holes can be provided on the oil injection needle 100, and the number of corresponding separation oil and gas chambers can be increased accordingly.

[0025] The technical solution of the present invention is further described below in conjunction with specific embodiments:

[0026] A micro-droplet chip gas-liquid driving device includes an air pump (i.e., air pump 14, the same below), a main air cavity (i.e., main air cavity 13, the same below), a floating oil-gas cavity (i.e., floating oil-gas cavity 11, the same below), a first separated oil-gas cavity (i.e., first separated oil-gas cavity 12, the same below), a second separated oil-gas cavity (i.e., second separated oil-gas cavity 17, the same below), an oil bottle (i.e., oil bottle 51, the same below), a plunger pump (i.e., oil pump 52, the same below), an oil storage pipe (i.e., oil storage pipe 21, the same below), etc. The air pump inhales air from the air, and the gas enters the main air cavity through a filter (i.e., filter 15, the same below), and enters three separate air cavities (i.e., floating oil-gas cavity 11, first separated oil-gas cavity 12, and second separated oil-gas cavity 17) through pipelines and solenoid valves. The main air cavity, floating oil-gas cavity, first separated oil-gas cavity, and second separated oil-gas cavity are respectively provided with pressure sensors, pressure relief valves, etc., which can correct the air cavity pressure in real time and dynamically keep the pressure of each air cavity constant. The plunger pump draws liquid from the oil bottle and injects a quantitative amount of detection oil into the oil storage pipe. The gas with constant pressure in the air cavity pushes the quantitative detection oil into the micro-droplet chip. The micro-droplet chip gas-liquid drive module pushes the detection oil of constant flow into the micro-droplet chip with constant air pressure, so that the droplet flow rate in the chip is stable and the interval is uniform, and there will be no droplet adhesion and other phenomena, so that the measured signal peak width meets the algorithm recognition requirements.

[0027] For details, see Figure 1As shown, the air pump inhales air from the air, and the air passes through a filter to filter out moisture and dust in the air, and enters the main air chamber through a pipeline. The main air chamber is connected to the floating oil and air chamber, the first separated oil and air chamber, and the second separated oil and air chamber through three air pipes. Each of the four air chambers has a pressure sensor and a pressure relief valve to monitor the air pressure in the air chamber in real time, dynamically ensuring that the pressure in each air chamber is constant. When the air pressure in a certain air chamber among the floating oil and air chamber, the first separated oil and air chamber, and the second separated oil and air chamber is lower than the set value, the solenoid valve between the air chamber and the main air chamber opens, and the gas in the main air chamber enters the air chamber to increase the air pressure until it rises to the set value of the air chamber. The solenoid valve between the air chamber and the main air chamber is closed. When the gas overshoots and causes the pressure to be higher than the set value, the relief valve opens to relieve the pressure, so that the pressure value drops to the set value. When the air pressure in the main air cavity is lower than the set value, the air pump starts to supply air to the main air cavity, causing the air pressure to increase until it rises to the set value of the main air cavity. The air pump is turned off. When the gas overshoots and causes the pressure to be higher than the set value, the relief valve opens to release the pressure, causing the pressure value to drop to the set value.

[0028] Continuing, when there is no test oil (i.e. the aforementioned oil) in the liquid circuit, during the initial filling, the solenoid valve in front of the plunger pump opens, the plunger pump draws the test oil from the oil bottle and injects it into the busbar, and at the same time, the three-way valves above and below the oil storage pipe are opened in pairs (the two three-way valves above and below one oil storage pipe are a pair, Figure 1 There are four pairs in total). The oil level rises along the oil storage pipe, first passes through the lower bubble sensor, and then the liquid level continues to rise. During the filling process, the air in the oil storage pipe is discharged toward the waste liquid tank through the three-way valve above the oil storage pipe. When the liquid level in the oil storage pipe reaches the upper bubble sensor, the three-way valve below the oil storage pipe is switched, and the liquid in the oil storage pipe flows toward the direction of the oil filling needle. At this time, the solenoid valve between the oil filling needle and the bus is in a closed state.

[0029] When the gas pushes the liquid, the three-way valve above the oil storage pipe is activated to connect the gas path with the oil storage pipe. At the same time, the solenoid valve between the bus and the oil injection needle is opened, and the gas in the floating oil-gas chamber, the first separation oil-gas chamber, and the second separation oil-gas chamber enters the oil storage pipe along the pipeline. Under the action of gas pressure, the detection oil level in the oil storage pipe drops, and the detection oil enters the oil injection needle and finally enters the micro-droplet chip, driving the droplets in the chip to move and separate for detection. When the liquid level in the oil storage pipe drops to the position of the lower bubble sensor, the three-way valve above the oil storage pipe is activated to connect the oil storage pipe with the pipeline in the direction of the waste liquid tank, and at the same time, the solenoid valve between the bus and the oil injection needle is closed. When a channel detection is completed, the chip will move a channel position to make the floating oil needle hole and the separation oil needle hole of the oil injection needle align with the new micro-droplet chip channel.

[0030] Continuing, the solenoid valve in front of the plunger pump opens, and the plunger pump draws the test oil from the oil bottle and injects it into the bus. At the same time, the three-way valves above and below the oil storage tube open in pairs, and the test oil level rises from the lower bubble sensor position along the oil storage tube. When the liquid level in the oil storage tube reaches the upper bubble sensor, the three-way valve below the oil storage tube is switched, so that the oil storage tube is connected to the pipeline in the direction of the oil injection needle. At this time, the solenoid valve between the oil injection needle and the bus is in a closed state. When the gas pushes the liquid, the gas enters the oil storage tube from the air cavity, the test oil in the oil storage tube enters the oil injection needle, and finally enters the micro-droplet chip, driving the droplets in the chip to move and separate for detection. Repeat the above oil storage tube filling and gas pushing liquid process until the sample droplets in all channels in the micro-droplet chip are detected.

[0031] It needs to be further explained that when the droplet samples in the micro-droplet chip are tested, the floating oil is needed to float the droplets from the storage tank in the chip, and then push the droplets to flow in a row. The separation oil separates the adjacent droplets one by one, so that the adjacent droplets maintain approximately the same distance, avoiding two droplets being completely close to each other during testing. The flow rate of the test oil can be determined by determining the gas and liquid in each air cavity, and then the flow rate of the sample droplets and the spacing of the droplet parts can be determined, so that the peak width and duty cycle of the measured signal can be controlled. The position of the upper bubble sensor above each oil storage tube can be adjusted according to the required amount of test oil. The capacity of the oil storage tube between the upper and lower bubble sensors is the amount of oil injected at one time.

[0032] Figure 1 The number of air chambers in is only one embodiment, and the number of branch air chambers can be increased or decreased according to specific needs, but the composition of the air chambers remains unchanged, that is, there is a main air chamber responsible for buffering the air pressure impact of the air pump, and there is a floating oil-gas chamber, the number of air paths divided from it is equal to the number of separated oil-gas chambers, and each separated oil-gas chamber has an air path output, or there is a separated oil-gas chamber, the number of air paths divided from it is equal to the number of floating oil-gas chambers, and each floating oil-gas chamber has an air path output so that the number of floating oil-gas paths is equal to the number of separated oil-gas paths.

[0033] In some embodiments, not all oil storage tubes need to be filled with oil, and then the gas pushes the liquid into the chip. It should be pointed out that the floating oil storage tube and the separation oil storage tube need to be filled with oil in pairs, and the gas pushes the liquid at the same time to complete the driving and separation of the sample droplets in the same chip channel. Therefore, when the micro-droplet chip gas-liquid driving module is working, there can be a pair of floating oil and separation oil liquid paths that can simultaneously push the liquid with gas, there can be two pairs of floating oil and separation oil liquid paths that can simultaneously push the liquid with gas, and there can also be multiple pairs of floating oil and separation oil liquid paths that can simultaneously push the liquid with gas.

[0034] Further explanation is needed. Figure 1The gas-liquid schematic diagram shown has no clear restrictions on the shape, size, and layout of each component, but the gravity of the test oil needs to be taken into account in some positions. For example, the change in the liquid level of the test oil in the oil storage tube requires that there is no dead cavity in the oil storage tube when the test oil is injected into the oil storage tube. The upper and lower bubble sensors are indeed located above and below in the vertical direction. The waste liquid tank is located below the three-way valve on the oil storage tube so that the overflowed test oil can flow along the tube into the waste liquid tank under the action of gravity. The oil injection needle is located below the micro-droplet chip.

[0035] When the micro-droplet chip gas-liquid drive device is not used for a long time, the gas circuit pressure needs to be reset to zero, and the detection oil in the liquid circuit needs to be discharged in order to increase the life of the device. First of all, it can be confirmed that the current state is that the liquid level of the detection oil in the oil storage pipe is at the position of the lower bubble sensor. At this time, the plunger pump reverses, and the gas enters from the air pipe in the waste liquid tank and enters the oil storage pipe. The detection oil stored in the lower bubble sensor and the plunger pump enters the plunger pump and is discharged into the oil bottle. Then the two three-way valves above and below the oil storage pipe are opened, and the detection oil stored between the three-way valve below the oil storage pipe and the oil filling needle flows out from the needle hole of the oil filling needle. At this time, there is no detection oil in the liquid circuit, and then all the pressure relief valves of all air cavities are opened to release pressure until it is the same as the atmospheric pressure value, and then the pressure relief valve is closed to prevent external dust from entering the air cavity.

[0036] It is easy for those skilled in the art to understand that, under the premise of no conflict, the above-mentioned advantageous methods can be freely combined and superimposed.

[0037] The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention shall be included in the protection scope of the present invention. The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and variations can be made without departing from the technical principles of the present invention, and these improvements and variations should also be regarded as the protection scope of the present invention.

Claims

1. A micro-droplet chip gas-liquid driving device, characterized in that: The invention comprises an oil supply component, an air supply source component, and an oil injection needle (100); the air supply source component comprises an upward oil-gas cavity (11) and a first separated oil-gas cavity (12); the pressure airflow output by the air supply source component can be controlled to enter the upward oil-gas cavity (11) and the first separated oil-gas cavity (12) respectively; the oil injection needle (100) has a first upward oil needle hole (101) and a first separated oil needle hole (102); a first pipeline is provided between the first upward oil needle hole (101) and the upward oil-gas cavity (11); the first separated oil needle hole (102) and the first separated oil-gas cavity (12) are connected to each other; A second pipeline is provided between the first pipeline and the second pipeline, and an oil storage component is provided on both the first pipeline and the second pipeline. The oil supply component can be controlled to supply oil to the oil storage component. The oil in the oil storage component on the first pipeline can be transported to the first floating oil needle hole (101) under the action of the floating oil and gas chamber (11), and the oil in the oil storage component on the second pipeline can be transported to the first separating oil needle hole (102) under the action of the first separating oil and gas chamber (12); the oil storage component includes a vertically arranged oil storage pipe (21), the storage A first electromagnetic three-way valve (22) is provided at the upper end of the oil pipe (21), and a second electromagnetic three-way valve (23) is provided at the lower end of the oil storage pipe (21), wherein the first electromagnetic three-way valve (22) is configured to connect the floating oil-gas cavity (11) or the first separated oil-gas cavity (12) with the corresponding oil storage pipe (21) when the air supply source component is required to push the oil in the oil storage pipe (21), and to connect the oil storage pipe (21) with the waste liquid tank (3) when the oil supply component is required to supply oil into the oil storage pipe (21), and the second electromagnetic three-way valve (23) is configured to When the air supply source component is required to push the oil in the oil storage pipe (21), the oil storage pipe (21) and the first floating oil needle hole (101) or the first separating oil needle hole (102) are connected; when the oil supply component is required to supply oil into the oil storage pipe (21), the oil storage pipe (21) and the oil supply component are connected; when oil supply is required, the air supply source component is controlled to push the oil stored in the oil storage pipe (21) into the corresponding channel of the micro-droplet chip (200) through the pressure gas stored in the floating oil gas cavity (11) and the first separating oil gas cavity (12).

2. The micro-droplet chip gas-liquid driving device according to claim 1, characterized in that: An upper bubble sensor (24) is provided between the upper end of the oil storage pipe (21) and the first electromagnetic three-way valve (22), and a lower bubble sensor (25) is provided between the lower end of the oil storage pipe (21) and the second electromagnetic three-way valve (23).

3. The micro-droplet chip gas-liquid driving device according to claim 1, characterized in that: The air supply source assembly further comprises a main air chamber (13), wherein the main air chamber (13) is controllably connected to the floating oil and air chamber (11) via a first solenoid valve (131), and the main air chamber (13) is controllably connected to the first separated oil and air chamber (12) via a second solenoid valve (132). The air supply source assembly further comprises an air pump (14) and a filter (15) connected between the air pump (14) and the main air chamber (13).

4. The micro-droplet chip gas-liquid driving device according to claim 3, characterized in that: The main air chamber (13), the floating oil and air chamber (11) and the first separated oil and air chamber (12) are respectively provided with a pressure sensor (161) and a pressure relief valve (162).

5. The micro-droplet chip gas-liquid driving device according to claim 1, characterized in that: The oil outlet of the oil supply assembly is controllably connected to the bus bar (4), and the bus bar (4) is also connected to the first pipeline and the second pipeline.

6. The micro-droplet chip gas-liquid driving device according to claim 5, characterized in that: The oil supply assembly comprises an oil bottle (51) and an oil pump (52); the oil pump (52) is capable of pumping the oil in the oil bottle (51) to the bus bar (4) and injecting the oil into the oil storage pipe (21) via the bus bar (4).

7. The micro-droplet chip gas-liquid driving device according to claim 6, characterized in that: A third solenoid valve (53) is provided between the oil bottle (51) and the oil pump (52).

8. The micro-droplet chip gas-liquid driving device according to claim 1, characterized in that: A fourth solenoid valve (6) is provided on the pipeline between the second solenoid three-way valve (23) and the first floating oil needle hole (101) or the first separation oil needle hole (102).

9. The micro-droplet chip gas-liquid driving device according to claim 3, characterized in that: The oil injection needle (100) is provided with a second floating oil needle hole (103) and a second separation oil needle hole (104); the air supply source component also includes a second separation oil and gas chamber (17) controllably connected to the main air chamber (13); a third pipeline is provided between the second separation oil needle hole (104) and the second separation oil and gas chamber (17); a fourth pipeline is provided between the second floating oil needle hole (103) and the floating oil and gas chamber (11); and the oil storage component is provided on both the third pipeline and the fourth pipeline.

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