Eyewash containing stable antioxidant enzyme and preparation method thereof

Abstract

The present invention discloses an eyewash containing a stable antioxidant enzyme and a preparation method thereof, comprising the following components in percentage by weight: 0.001-1.0% of a stable antioxidant enzyme; 0.01-1.0% of sodium hyaluronate; 0.05-5.0% of trehalose; 0.2-0.4% of taurine; 0.001-0.5% of a cooling agent; 0.1-0.2% of disodium edetate; 0.6-0.9% of sodium chloride; 0.1-0.8% of boric acid; 0.01-0.2% of borax; 0.0001-0.2% of a bactericidal preservative; and the balance being purified water. The present invention can effectively reduce the occurrence of dry eye symptoms and reduce or alleviate the discomfort caused by dry eye.

Description

Technical Field

[0001] The present invention relates to the technical field of eyewash liquids, and in particular to an eyewash liquid containing stable antioxidant enzymes and a preparation method thereof. Background Art

[0002] With the development of society and technology, people obtain more information through reading electronic devices. The side effect of this is that the contact time of the eyes gradually increases, causing the eyes to come into contact with mobile phone screens, computer screens, and projection equipment for a long time, causing the eyes to have varying degrees of dry eye symptoms. When dry eye occurs, the later stages basically rely on artificial tear products to relieve the discomfort caused by it, but they are often unable to cure it. In addition, the moisturizing effect of the eyewash currently on the market is low. Usually, after washing the eyes or applying eye drops, the eyeballs will become dry again in a short time. Therefore, it is necessary to propose an eyewash that can effectively prevent or reduce the occurrence of dry eye, and can effectively relieve the discomfort symptoms when dry eye occurs. Summary of the Invention

[0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide an eyewash containing stable antioxidant enzymes to solve the technical problems of the prior art eyewashes such as low applicability, strong irritation and poor lubrication and moisturizing.

[0004] To achieve the above object, the present invention provides an eyewash solution containing a stable antioxidant enzyme, comprising the following components in percentage by weight:

[0005] Stable antioxidant enzyme 0.001-1.0%;

[0006] Sodium hyaluronate 0.01-1.0%;

[0007] Trehalose 0.05-5.0%;

[0008] Taurine 0.2-0.4%;

[0009] Cooling agent 0.001-0.5%;

[0010] Edetate disodium 0.1-0.2%;

[0011] Sodium chloride 0.6-0.9%;

[0012] Boric acid 0.1-0.8%;

[0013] Borax 0.01-0.2%;

[0014] Bactericidal preservative 0.0001-0.2%;

[0015] The balance is purified water.

[0016] Further, as preferred, the following components are included in percentage by weight:

[0017] Stable antioxidant enzyme 0.01-0.5%;

[0018] Sodium hyaluronate 0.1-0.5%;

[0019] Trehalose 0.5-1.0%;

[0020] Taurine 0.25-0.35%;

[0021] Cooling agent 0.01-0.05%;

[0022] Edetate disodium 0.1-0.15%;

[0023] Sodium chloride 0.6-0.8%;

[0024] Boric acid 0.1-0.7%;

[0025] Borax 0.01-0.1%;

[0026] Bactericidal preservative 0.0001-0.15%;

[0027] The balance is purified water.

[0028] Further, as more preferably, the following components are included in percentage by weight:

[0029] Stable antioxidant enzyme 0.01%;

[0030] Sodium hyaluronate 0.16%;

[0031] Trehalose 0.5%;

[0032] Taurine 0.30%;

[0033] Cooling agent 0.02%;

[0034] Edetate disodium 0.12%;

[0035] Sodium chloride 0.7%;

[0036] Boric acid 0.45%;

[0037] Borax 0.05%;

[0038] Bactericidal preservative 0.0015%;

[0039] The balance is purified water.

[0040] The present invention also provides a method for preparing an eyewash solution containing a stable antioxidant enzyme, comprising the following steps:

[0041] S1. Take an appropriate amount of purified water, add sodium hyaluronate to the purified water, heat appropriately to dissolve, mix evenly, and let the solution cool down before use;

[0042] S2. Take an appropriate amount of purified water, add trehalose, taurine, disodium edetate, sodium chloride, boric acid, and borax, dissolve at room temperature, mix evenly, then add a cooling agent and a bactericidal preservative; after stirring evenly, add the reserved sodium hyaluronate solution and mix evenly, finally add the stabilized antioxidant enzyme and mix evenly, and add purified water to the full amount;

[0043] S3. Sterilize and filter the solution prepared in S2, and divide the filtrate into smaller packages. The preparation is complete.

[0044] Furthermore, the stable antioxidant enzyme is a high-temperature resistant superoxide dismutase SOD.

[0045] Furthermore, the molecular weight of the sodium hyaluronate is 500,000 to 1,500,000.

[0046] Furthermore, the cooling agent is one or both of menthol and borneol.

[0047] The present invention has the following beneficial effects: Compared with the prior art, the eyewash solution provided by the present invention contains a stable antioxidant enzyme. The stable antioxidant enzyme is used as an antioxidant, which can effectively prevent and / or reduce the probability of oxidative stress in the eye. Sodium hyaluronate is used as a lubricating and moisturizing agent, which has good biocompatibility and lubricity, enhancing comfort and lubrication, and can relieve dry eyes and other discomfort for a long time. The cooling agent can have a cooling effect, relieving symptoms of eye swelling and pain. The eyewash solution of the present invention can effectively reduce the occurrence of dry eye symptoms and reduce or alleviate the discomfort caused by dry eye.

[0048] The features and advantages of the present invention will be described in detail through embodiments. DETAILED DESCRIPTION

[0049] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention is further described in detail below through examples. However, it should be understood that the specific embodiments described herein are merely for the purpose of explaining the present invention and are not intended to limit the scope of the present invention. In addition, in the following description, descriptions of well-known structures and technologies are omitted to avoid unnecessary confusion of the concept of the present invention.

[0050] Example 1

[0051] An eyewash solution containing stable antioxidant enzymes comprises the following components in percentage by weight:

[0052] Stable antioxidant enzyme 0.01%;

[0053] Sodium hyaluronate 0.16%;

[0054] Trehalose 0.5%;

[0055] Taurine 0.30%;

[0056] Menthol 0.02%;

[0057] Edetate disodium 0.12%;

[0058] Sodium chloride 0.7%;

[0059] Boric acid 0.45%;

[0060] Borax 0.05%;

[0061] Polyaminopropyl biguanide 0.0015%;

[0062] The balance is purified water.

[0063] The eyewash solution is prepared by the following steps:

[0064] S1. Take an appropriate amount of purified water, add sodium hyaluronate to the purified water, heat appropriately to dissolve, mix evenly, and let the solution cool down before use;

[0065] S2. Take an appropriate amount of purified water, add trehalose, taurine, disodium edetate, sodium chloride, boric acid, and borax, dissolve at room temperature, mix evenly, then add borneol and polyaminopropyl biguanide; after stirring evenly, add the reserved sodium hyaluronate solution and mix evenly, finally add the stabilized antioxidant enzyme and mix evenly, and add purified water to the full amount;

[0066] S3. Sterile filter the solution prepared in S2 and divide the filtrate into aliquots.

[0067] Example 2

[0068] An eyewash solution containing stable antioxidant enzymes comprises the following components in percentage by weight:

[0069] Stable antioxidant enzyme 0.01%;

[0070] Sodium hyaluronate 0.16%;

[0071] Trehalose 0.5%;

[0072] Taurine 0.30%;

[0073] Menthol 0.02%;

[0074] Edetate disodium 0.12%;

[0075] Sodium chloride 0.7%;

[0076] Boric acid 0.45%;

[0077] Borax 0.05%;

[0078] Benzalkonium bromide 0.0015%;

[0079] The balance is purified water.

[0080] The method for preparing the eyewash solution comprises the following steps:

[0081] S1. Take an appropriate amount of purified water, add sodium hyaluronate to the purified water, heat appropriately to dissolve, mix evenly, and let the solution cool down before use;

[0082] S2. Take an appropriate amount of purified water, add trehalose, taurine, disodium edetate, sodium chloride, boric acid, and borax, dissolve at room temperature, mix evenly, then add menthol and benzalkonium bromide; after stirring evenly, add the reserved sodium hyaluronate solution and mix evenly, finally add the stabilized antioxidant enzyme and mix evenly, and add purified water to the full amount;

[0083] S3. Sterile filter the solution prepared in S2 and divide the filtrate into aliquots.

[0084] Example 3:

[0085] An eyewash solution containing stable antioxidant enzymes comprises the following components in percentage by weight:

[0086] Stable antioxidant enzyme 0.01%;

[0087] Sodium hyaluronate 0.16%;

[0088] Trehalose 0.5%;

[0089] Taurine 0.30%;

[0090] Menthol 0.02%;

[0091] Borneol 0.02%;

[0092] Edetate disodium 0.12%;

[0093] Sodium chloride 0.7%;

[0094] Boric acid 0.45%;

[0095] Borax 0.05%;

[0096] Polyaminopropyl biguanide 0.0015%;

[0097] The balance is purified water.

[0098] The preparation method of the eyewash solution comprises the following steps:

[0099] S1. Take an appropriate amount of purified water, add sodium hyaluronate to the purified water, heat appropriately to dissolve, mix evenly, and let the solution cool down before use;

[0100] S2. Take an appropriate amount of purified water, add trehalose, taurine, disodium edetate, sodium chloride, boric acid, and borax, dissolve at room temperature, mix evenly, then add menthol, borneol, and polyaminopropyl biguanide; after stirring evenly, add the reserved sodium hyaluronate solution and mix evenly, finally add the stabilized antioxidant enzyme and mix evenly, and add purified water to the full amount;

[0101] S3. Sterile filter the solution prepared in S2 and divide the filtrate into aliquots.

[0102] Example 4: Induction of dry eye and testing of treatment effects

[0103] This test uses the solution prepared in Example 3. In order to evaluate the effect of the eyewash solution on tearing, an in vivo test was conducted under conditions related to dry eye syndrome.

[0104] New Zealand White rabbits weighing 2-2.5 kg were used for the experiments.

[0105] One drop of 1.0% atropine sulfate (AS) solution was instilled into both eyes of the animals three times a day (9:00 AM, 1:00 PM, and 5:00 PM) for 5 days to reduce tear production and induce dry eyes. Five minutes after each AS administration, 10 ml of eyewash solution was flushed into the right eye.

[0106] The animals underwent a Hillman I test at time 0 (before treatment) and on days 2, 3, 4, and 5 after the start of treatment. Tear secretion was measured daily during the test. Before each administration of the solution (AS and AG-Sol), a filter paper strip was placed in the inferior conjunctival fornix of each rabbit eye. The tear growth along the filter paper strip was measured and expressed in millimeters. The values obtained for eyes treated with atropine sulfate alone were compared with those obtained for eyes treated with the eyewash solution of the present invention.

[0107] Baseline tear secretion was measured in control animals not treated with the drug.

[0108] The results are shown in the table below. The experiment showed that the eye wash solution has a protective effect against dry eye attacks.

[0109]

[0110] Furthermore, on days 3, 4, and 5 after the start of treatment, the extent of corneal epithelial changes caused by dry eye was evaluated by staining the cornea with 10 microliters of a 1% w / w aqueous fluorescein solution and observing with a slit lamp equipped with a blue filter. In the case of epithelial changes, areas of color on the corneal surface were observed: fluorescence-positive eyes. The following table records the results obtained, the percentage of fluorescence-positive eyes among all treated eyes, and compares the percentage of fluorescence-positive eyes after treatment with the eyewash solution with the percentage of fluorescence-positive eyes in control animals treated with AS alone.

[0111] The following table shows the results of slit lamp observation

[0112] As can be seen from the results shown in the table above, the percentage of fluorescence positivity in untreated eyes increased with the number of days and exceeded 60% on the 5th day of treatment, while in eyes treated with the eyewash solution, the percentage of fluorescence positivity remained constant between the 4th and 5th days, with a much lower value of approximately 16%.

[0113] The eyewash solution containing a stable antioxidant enzyme proposed by the present invention uses a stable antioxidant enzyme as an antioxidant, which can effectively prevent and / or reduce the probability of dry eyes caused by oxidative stress in the eyes; uses sodium hyaluronate as a lubricating and moisturizing agent, which has good biocompatibility and lubricity, enhances comfort and lubrication, and can relieve discomfort such as dry eyes for a long time; and uses a cooling agent to have a cooling effect, relieving symptoms of eye swelling and pain. The eyewash solution of the present invention can effectively reduce the occurrence of dry eye symptoms and reduce or alleviate the discomfort caused by dry eye.

[0114] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions or improvements made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An eyewash solution containing a stable antioxidant enzyme, characterized in that: It is composed of the following components in percentage by weight: Stable antioxidant enzyme 0.01%; Sodium hyaluronate 0.16%; Trehalose 0.5%; Taurine 0.30%; Menthol 0.02%; Borneol 0.02%; Edetate disodium 0.12%; Sodium chloride 0.7%; Boric acid 0.45%; Borax 0.05%; Polyaminopropyl biguanide 0.0015%; The balance is purified water; The stable antioxidant enzyme is a high-temperature resistant superoxide dismutase SOD; The molecular weight of the sodium hyaluronate is 500,000 to 1,500,000.

2. The method for preparing an eyewash solution containing a stable antioxidant enzyme according to claim 1, wherein: The steps include: S1. Take an appropriate amount of purified water, add sodium hyaluronate to the purified water, heat appropriately to dissolve, mix evenly, and let the solution cool down before use; S2. Take an appropriate amount of purified water, add trehalose, taurine, disodium edetate, sodium chloride, boric acid, and borax, dissolve at room temperature, mix evenly, then add a cooling agent and a bactericidal preservative; after stirring evenly, add the reserved sodium hyaluronate solution and mix evenly, finally add the stabilized antioxidant enzyme and mix evenly, and add purified water to the full amount; S3. The solution prepared in S2 is sterilized and filtered to complete the preparation, thereby obtaining the eyewash solution according to claim 1.

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