An active unwinding device and unwinding method for observing freely moving animals

Abstract

This invention provides an active unwinding device and method for observing freely moving animals. The unwinding device includes a test frame, a top plate, a bottom plate, a stepper motor, a drive assembly, a data acquisition unit, a sensing device, a conductive slip ring, a support frame, a connecting rod, and a controller. The bottom plate and top plate are fixed to both ends of the test frame. The output end of the stepper motor passes through the top plate, and the rotor of the conductive slip ring also passes through the top plate. The drive assembly includes a driving gear and a driven gear. The driving gear is mounted on the output end of the stepper motor. The driven gear meshes with the driving gear and is connected to the rotor of the conductive slip ring on the lower surface of the top plate. One end of the support frame is connected to the driven gear, and the other end is connected to the data acquisition unit. One end of the connecting rod is connected to the driven gear, and the other end is connected to the sensing device. The controller is fixedly mounted on the upper surface of the top plate. This invention can automatically unwind connecting lines that become entangled / twisted during experiments with mice in free movement.

Description

Technical Field

[0001] This invention relates to the field of automatic control unwinding technology, specifically to an active unwinding device and method for observing freely moving animals. Background Technology

[0002] Miniature fluorescence microscopy is a technique for fluorescence imaging of neuronal populations in freely moving small animals, making significant contributions to the development of neuroscience. By fixing a miniaturized microscope to the head, the microscope can image the activity of up to hundreds of neurons in the field of view in real time while the small animal is moving freely. The head-mounted device transmits signals via cables, but during the mouse's free movement, the cables can become tangled / twisted, thus restricting the mouse's movement, affecting the observation results, and causing many inconveniences. Summary of the Invention

[0003] In view of the problems existing in the existing technical solutions, the purpose of this invention is to provide an active unwinding device and unwinding method for observing freely moving animals.

[0004] To achieve the above objectives, the present invention provides the following technical solution:

[0005] An active unwinding device for observing freely moving animals, used to unwind the tangled / twisted connector between the head-mounted miniature fluorescence microscope of the observed live animal and the data acquisition device, comprising:

[0006] Testing framework;

[0007] Top plate, which is fixed to the top of the test frame;

[0008] A base plate, which is fixed to the bottom of the test frame;

[0009] The test frame, top plate, and bottom plate form an activity space for live animals;

[0010] The active unwinding device for observing freely moving animals also includes:

[0011] A stepper motor, with its output shaft penetrating the surface of the top plate;

[0012] A conductive slip ring is mounted on the upper surface of the top plate, and the rotor of the conductive slip ring penetrates the surface of the top plate.

[0013] A drive assembly includes a drive gear and a driven gear; the shaft of the drive gear is mounted on the output end of a stepper motor on the lower surface of the top plate; the driven gear meshes with the drive gear; the shaft of the driven gear is connected to the rotor of a conductive slip ring on the lower surface of the top plate.

[0014] A support frame, which is fixed to the tooth surface of the driven gear facing the active space.

[0015] The data acquisition device is fixedly connected to the other end of the support frame, and the data acquisition device is electrically connected to the image sensor of the miniature fluorescence microscope device worn on the head of a live animal via a connector;

[0016] A connecting rod is fixedly connected to the tooth surface of the driven gear facing the movable space end and is adjacent to the support frame;

[0017] A sensing device is rotatably connected to the other end of a connecting rod; the sensing device is connected to the connector below the data acquisition device, and the sensing device measures the rotation angle or torque generated when the live animal drives the sensing device to rotate on the connecting rod through the connector;

[0018] The controller is fixedly installed on the upper surface of the top plate; the signal line of the sensing device is electrically connected to the controller through a conductive slip ring to transmit the measured rotation angle information to the controller.

[0019] As a further aspect of this solution, the connector is a cord-type connector, and there is at least one cord-type connector.

[0020] As a further aspect of this solution, the connector is an FPC flexible wire or any type of transmission line; the number of FPC flexible wires is equal to the number of miniature fluorescence microscopes on the head of a live animal.

[0021] As a further aspect of this solution, the test frame, top plate, and bottom plate form a cuboid space for the activity of the live animal; the stepper motor is mounted on the upper surface of the top plate.

[0022] As a further aspect of this solution, the diameter of the driven gear is twice the diameter of the driving gear.

[0023] As a further improvement to this solution, the support frame is a hollow structure.

[0024] As a further aspect of this solution, the sensing device is an angle sensor; the angle sensor is located below the data acquisition unit.

[0025] As a further aspect of this solution, the connecting rod is L-shaped.

[0026] As a further step of this solution, the top plate is fixed to the top of the test frame by several angle steels; the bottom plate is fixed to the bottom of the test frame by several angle steels.

[0027] This invention also discloses an unwinding method for unwinding the entangled / twisted connector between the head-mounted miniature fluorescence microscope of the observed live animal and the data acquisition device, the steps of which are as follows:

[0028] (a) Before the live animal moves freely, the connection is in a state of no force, i.e. no entanglement / twisting occurs, and the signal value of the sensing device is recorded as the initial signal value;

[0029] (b) When the live animal moves freely, the live animal will entangle / twist with the connector, which will cause the sensor to rotate or generate torque, and the signal value of the sensor will change.

[0030] (c) The sensing device converts the signal generated by the winding / twisting of the connector into an electrical signal and transmits it to the controller through the signal line;

[0031] (d) The controller receives the signal and makes a judgment. When the real-time signal value of the sensing device deviates from the initial signal value, the controller controls the unwinding device to rotate and unwind in the same direction as the rotation direction of the live animal.

[0032] (e) The sensing device periodically sends electrical signals to the controller to record the winding state of the connector;

[0033] (f) When the signal value of the sensing device reaches the initial signal value, the winding / twisting is unwound, and the controller controls the unwinding device to stop rotating.

[0034] Specifically, the unwinding method for observing the above-mentioned active unwinding device of freely moving animals includes the following unwinding steps:

[0035] (a) Before the live animal moves freely, record the signal value of the sensing device when the connector is not under force, i.e., without entanglement / twisting, and record it as the initial signal value;

[0036] (b) When the live animal moves freely, the live animal will entangle / twist with the connector, and the connector will cause the sensing device to rotate or generate torque on the connecting rod;

[0037] (c) The sensing device converts the signal generated on the connecting rod due to winding / twisting into an electrical signal, which is then transmitted to the controller through the signal line of the conductive slip ring;

[0038] (d) After receiving the signal, the controller controls the stepper motor to rotate in the opposite direction to the rotation direction of the live animal;

[0039] (e) The stepper motor drives the drive gear to rotate in the opposite direction to the rotation of the live animal; the drive gear drives the driven gear to rotate in the same direction as the rotation of the live animal;

[0040] (f) The driven gear drives the data acquisition unit and the sensing device to rotate in the same direction as the rotation of the live animal, thereby beginning to untangle the tangled / twisted connection;

[0041] (g) The sensing device records the electrical signal of the winding state of the connector to the controller in real time;

[0042] (h) When the signal value of the sensing device reaches the initial signal value, the winding / twisting is unwound, and the controller controls the stepper motor to stop rotating.

[0043] Compared with the prior art, the beneficial effects of the present invention are: 1. The unwinding method of the active unwinding device for observing freely moving animals is to drive the data acquisition device on the driven gear by the stepper motor to drive the active gear, thereby eliminating the torque generated by the FPC soft wire that is entangled / twisted during the movement of the live animal.

[0044] 2. The active unwinding device for observing free-moving animals eliminates the need for the experimenter to observe and judge the direction of cable entanglement / twist before manually controlling the motor to achieve unwinding. Once the cable becomes entangled / twisted, the unwinding mechanism responds immediately to unwind it, enabling immediate unwinding of entangled / twisted cables. This is more sensitive, stable, and saves manpower and resources while ensuring that the experiment can continue more effectively.

[0045] 3. The unwinding method of the active unwinding device for observing freely moving animals can achieve the goal of not requiring human supervision during the experiment, so that the behavior of the live animals is not affected by humans, making the experimental results more accurate and ensuring the reliability of the experimental data. Attached Figure Description

[0046] The invention will be further described below with reference to the accompanying drawings.

[0047] Figure 1 This is a front view structural diagram of the present invention;

[0048] Figure 2 Top view of the structure of this invention;

[0049] Figure 3 yes Figure 2 Schematic diagram of the structure at point A in the middle;

[0050] Figure 4 Schematic diagram of mouse movement and unrotation in Embodiment 1 of the present invention;

[0051] Figure 5 Schematic diagram of mouse movement and unrotation in Example 2 of this invention.

[0052] The diagram is labeled as follows: 1-Test frame, 2-Top plate, 3-Bottom plate, 4-Stepper motor, 5-Drive assembly, 51-Driven gear, 52-Driven gear, 6-Data acquisition unit, 7-Sensing device, 8-Conductive slip ring, 9-Support frame, 10-Connecting rod, 11-Controller. Detailed Implementation

[0053] The technical solution of the present invention will be clearly and completely described below with reference to the embodiments and accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments.

[0054] like Figure 1-3 As shown, the present invention provides an active unwinding device for observing freely moving animals, used to unwind the entangled / twisted connection between the head-mounted miniature fluorescence microscope of the observed live animal and the data acquisition device, including a test frame 1, a top plate 2, a bottom plate 3, a stepper motor 4, a drive assembly 5, a data acquisition device 6, a sensing device 7, a conductive slip ring 8, a support frame 9, a connecting rod 10, and a controller 11.

[0055] The connector is a cord-like connector, and there is at least one cord-like connector. Specifically, the connector is an FPC flexible wire; the number of FPC flexible wires is equal to the number of miniature fluorescence microscopes on the live animal's head; or the number matches the number of miniature fluorescence microscopes on the live animal's head according to a predetermined number; the connector can be any type of transmission line, as long as it is entangled / twisted, it is the object of the unwinding device of this invention.

[0056] The top plate 2 is fixed to the top of the test frame 1 by several angle steels.

[0057] The base plate 3 is fixed to the bottom of the test frame 1 by several angle steels.

[0058] The test frame 1, top plate 2, and bottom plate 3 form a cuboid space for the moving animal.

[0059] The output end of the stepper motor 4 penetrates the surface of the top plate 2.

[0060] The conductive slip ring 8 is installed on the upper surface of the top plate 2, and the rotor of the conductive slip ring 8 penetrates the surface of the top plate 2.

[0061] The drive assembly 5 includes a drive gear 52 and a driven gear 51; the shaft of the drive gear 52 is mounted on the output end of the stepper motor 4 on the lower surface of the top plate 2; the driven gear 51 meshes with the drive gear 52; the shaft of the driven gear 51 is connected to the rotor of the conductive slip ring 8 on the lower surface of the top plate 2.

[0062] The support frame 9 is fixed on the tooth surface of the driven gear 51 facing the end of the moving space.

[0063] The data acquisition unit 6 is fixedly connected to the other end of the support frame 9. The data acquisition unit 6 is electrically connected to the image sensor of the miniature fluorescence microscope device worn on the head of a live animal through several FPC flexible wires.

[0064] The connecting rod 10 is fixedly connected to the tooth surface of the driven gear 51 facing the active space and is adjacent to the support frame 9.

[0065] The sensing device 7 is rotatably connected to the other end of the connecting rod 10; the sensing device 7 is connected to the FPC cable below the data acquisition device, and the sensing device 7 measures the rotation angle of the living animal on the connecting rod 10 driven by the FPC cable.

[0066] The sensing device 7 is an angle sensor. It is used to measure the rotation angle of the live animal as it moves along the connecting rod 10 via the FPC cable. The sensing device can be any type of connector that records the cable winding state.

[0067] The controller 11 is fixedly installed on the upper surface of the top plate 2; the signal line of the angle sensing device 7 is electrically connected to the controller 11 through the conductive slip ring 8, and transmits the measured rotation angle information to the controller 11.

[0068] The stepper motor 4 is mounted on the upper surface of the top plate 2. The diameter of the driven gear 51 is twice the diameter of the driving gear 52. The support frame 9 has a hollow structure. The angle sensing device 7 is located below the data acquisition unit 6. The connecting rod 10 is L-shaped.

[0069] The specific operating principle is as follows: before the live animal moves freely, the angle signal value of the sensing device 7 is recorded when the connector is not under force, that is, when there is no entanglement / twisting, and this value is recorded as the initial angle signal value. When the live animal moves freely, it will become entangled / twisted with the connector, causing the connector to rotate on the connecting rod 10. The angle sensing device 7 converts the rotation angle signal on the connecting rod 10 into a digital signal and transmits it to the controller 11 through the signal line of the conductive slip ring 8. After receiving the signal, the controller 11 controls the stepper motor 4 to rotate in the opposite direction to the live animal's rotation. The stepper motor 4 drives the drive gear 52 to rotate in the opposite direction to the live animal's rotation. The drive gear 52 drives the driven gear 51 to rotate in the same direction as the live animal's rotation. The driven gear 51 drives the data acquisition device 6 and the sensing device 7 to rotate in the same direction as the live animal's rotation, thus starting to untangle / twist the connector. The sensing device 7 sends an angle signal to the controller 11 in real time. When the angle signal value of the sensing device 7 reaches or approaches (within ±5° of the initial angle signal value) the initial angle signal value, the entanglement / twist is untangled, and the controller 11 controls the stepper motor 4 to stop rotating.

[0070] like Figure 1-3 As shown, the present invention also provides an unwinding method applied to the above-mentioned unwinding device, the steps of which are as follows:

[0071] (a) Before the live animal moves freely, record the angle signal value of the sensing device 7 when the connector is not under force, i.e., when there is no entanglement / twisting, and record it as the initial angle signal value.

[0072] (b) When the live animal moves freely, the live animal will entangle / twist with the connecting line, and the connector will cause the angle sensing device 7 to rotate on the connecting rod 10.

[0073] (c) The sensing device 7 converts the rotation angle signal on the connecting rod 10 into a digital signal and transmits it to the controller 11 through the signal line of the conductive slip ring 8;

[0074] (d) After receiving the signal, the controller 11 controls the stepper motor 4 to rotate in the opposite direction to the rotation direction of the live animal;

[0075] (e) Stepper motor 4 drives drive gear 52 to rotate in the opposite direction to the rotation direction of the live animal; drive gear 52 drives driven gear 51 to rotate in the same direction as the rotation direction of the live animal;

[0076] (f) Driven gear 51 drives data acquisition unit 6 and sensing device 7 to rotate in the same direction as the rotation of the live animal, thereby starting to untangle the tangled / twisted connection; (g) The sensing device 7 sends an angle signal to the controller 11 in real time;

[0077] (h) When the angle signal value of the sensing device 7 reaches or approaches the initial angle signal value (within ±5° of the initial angle signal value), the winding / twisting is unwound, and the controller 11 controls the stepper motor 4 to stop rotating.

[0078] Example 1:

[0079] like Figure 4 As shown in the figure, this embodiment discloses an active unwinding device for observing freely moving animals. It is used to unwind the tangled / twisted FPC cable between the observed mouse's head-mounted miniature fluorescence microscope and the data acquisition unit. The device includes a test frame 1, a top plate 2, a bottom plate 3, a stepper motor 4, a drive assembly 5, a data acquisition unit 6, a sensing device 7, a conductive slip ring 8, a support frame 9, a connecting rod 10, and a controller 11. In this embodiment, the sensing device 7 is an angle sensor, used to observe the unwinding process of the unwinding device when the mouse performs clockwise rotational movements. The specific structure of this embodiment is as described above in the specific structural description of the present invention.

[0080] When the mouse moves clockwise within the cuboid-shaped activity space formed by the test frame 1, top plate 2, and bottom plate 3, the angle signal value of the sensing device 7 is recorded before the mouse begins free movement, under no-stress conditions (i.e., no entanglement / twisting). This initial angle signal value is recorded. As the mouse moves clockwise within this space, the FPC cable will entangle / twist clockwise, causing the angle sensor to rotate on the connecting rod 10. The angle sensor converts the rotation angle signal on the connecting rod 10 into a digital signal, which is transmitted to the controller 11 via the signal line of the conductive slip ring 8. After receiving the signal, the controller 11 controls the stepper motor 4 to rotate counterclockwise; the stepper motor 4 drives the drive gear 52 to rotate counterclockwise; the drive gear 52 drives the driven gear 51 to rotate clockwise; the driven gear 51 drives the data acquisition unit 6 and the angle sensor to rotate clockwise, thereby starting to unwind / twist the FPC cable; the angle sensor sends an angle signal to the controller 11 in real time; when the angle signal value of the angle sensor reaches or approaches (within ±5° of the initial angle signal value) the initial angle signal value, the winding / twisting is unwound, and the controller 11 controls the stepper motor 4 to stop rotating.

[0081] Example 2:

[0082] like Figure 5 As shown in the figure, this embodiment discloses an active unwinding device for observing freely moving animals. It is used to unwind the FPC flexible cable entangled / twisted between the observed mouse's head-mounted miniature fluorescence microscope and the data acquisition device. The device includes a test frame 1, a top plate 2, a bottom plate 3, a stepper motor 4, a drive assembly 5, a data acquisition device 6, a sensing device 7, a conductive slip ring 8, a support frame 9, a connecting rod 10, and a controller 11. In this embodiment, the sensing device 7 is an angle sensor, used to observe the unwinding process of the unwinding device when the mouse performs counterclockwise rotational movements. The specific structure of this embodiment is as described above in the specific structural description of the present invention.

[0083] When the mouse moves counterclockwise within the cuboid-shaped activity space formed by the test frame 1, top plate 2, and bottom plate 3, the angle signal value of the sensing device 7 is recorded before the mouse begins free movement, under no-stress conditions (i.e., no entanglement / twisting). This initial angle signal value is recorded. As the mouse moves counterclockwise within this space, the FPC cable will entangle / twist counterclockwise, causing the angle sensor to rotate on the connecting rod 10. The angle sensor converts the rotation angle signal on the connecting rod 10 into a digital signal. The signal line of the conductive slip ring 8 is transmitted to the controller 11; after receiving the signal, the controller 11 controls the stepper motor 4 to rotate clockwise; the stepper motor 4 drives the drive gear 52 to rotate clockwise; the drive gear 52 drives the driven gear 51 to rotate counterclockwise; the driven gear 51 drives the data acquisition unit 6 and the angle sensor to rotate counterclockwise, thereby starting to unwind / twist the FPC cable; the angle sensor sends an angle signal to the controller 11 in real time; when the angle signal value of the angle sensor reaches or approaches (within ±5° of the initial angle signal value) the initial angle signal value, the winding / twisting is unwound, and the controller 11 controls the stepper motor 4 to stop rotating.

[0084] Example 3:

[0085] like Figure 5 As shown in the figure, this embodiment discloses an active unwinding device for observing freely moving animals. It is used to unwind the tangled / twisted FPC cable between the observed mouse's head-mounted miniature fluorescence microscope and the data acquisition unit. The device includes a test frame 1, a top plate 2, a bottom plate 3, a stepper motor 4, a drive assembly 5, a data acquisition unit 6, a sensing device 7, a conductive slip ring 8, a support frame 9, a connecting rod 10, and a controller 11. In this embodiment, the sensing device 7 is an angle sensor, used to observe the unwinding process of the unwinding device when the mouse performs random alternating clockwise and counterclockwise movements. The specific structure of this embodiment is as described above in the specific structural description of the present invention.

[0086] When the mouse moves randomly in a clockwise and counterclockwise alternating motion in the cuboid activity space formed by the test frame 1, top plate 2 and bottom plate 3, the controller 11 controls the stepper motor 4 to repeat the response actions corresponding to Embodiments 1 and 2 above, and completes the unspinning.

[0087] The above description is merely an example and illustration of the structure of the present invention. Those skilled in the art can make various modifications or additions to the specific embodiments described, or use similar methods to replace them, as long as they do not deviate from the structure of the present invention or exceed the scope defined in the claims, all of which should fall within the protection scope of the present invention.

Claims

1. An active unwinding device for observing freely moving animals, used to unwind the tangled / twisted connector between the head-mounted miniature fluorescence microscope of the observed live animal and the data acquisition device, comprising: Test framework (1); Top plate (2), which is fixed to the top of the test frame (1); The base plate (3) is fixed to the bottom of the test frame (1); The test frame (1), top plate (2) and bottom plate (3) form an activity space for the live animal; The active unwinding device for observing freely moving animals is characterized in that it further includes: Stepper motor (4), the output shaft of stepper motor (4) passes through the surface of top plate (2); A conductive slip ring (8) is installed on the upper surface of the top plate (2), and the rotor of the conductive slip ring (8) penetrates the surface of the top plate (2). The drive assembly (5) includes a drive gear (52) and a driven gear (51); the shaft of the drive gear (52) is mounted on the output end of the stepper motor (4) on the lower surface of the top plate (2); the driven gear (51) meshes with the drive gear (52); the shaft of the driven gear (51) is connected to the rotor of the conductive slip ring (8) on the lower surface of the top plate (2); Support frame (9), the support frame (9) is fixed on the tooth surface of the driven gear (51) facing the end of the active space; The data acquisition device (6) is fixedly connected to the other end of the support frame (9). The data acquisition device (6) is electrically connected to the image sensor of the miniature fluorescence microscope device worn on the head of a live animal through a connector. The connecting rod (10) is fixedly connected to the tooth surface of the driven gear (51) facing the active space and is adjacent to the support frame (9); The sensing device (7) is connected to the other end of the connecting rod (10); the sensing device (7) is connected to the connector below the data collector (6), and the sensing device (7) measures the rotation angle or torque generated by the live animal driving the sensing device (7) to rotate through the connector. The controller (11) is fixedly installed on the upper surface of the top plate (2); the signal line of the sensing device (7) is electrically connected to the controller (11) through the conductive slip ring (8) to transmit the measured rotation angle information to the controller (11).

2. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The connector is a rope-type connector, and there is at least one rope-type connector.

3. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The connector is an FPC flexible wire or any type of transmission line; the number of FPC flexible wires is equal to the number of miniature fluorescence microscopes on the head of a live animal.

4. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The test frame (1), top plate (2) and bottom plate (3) form a cuboid space for the activity of the live animal, and the stepper motor (4) is installed on the upper surface of the top plate (2).

5. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The diameter of the driven gear (51) is twice the diameter of the driving gear (52).

6. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The sensing device (7) is a sensor that records the state of cable winding; the sensing device (7) is located below the data acquisition device (6).

7. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The connecting rod (10) is L-shaped.

8. The active unwinding device for observing freely moving animals according to claim 1, characterized in that... The top plate (2) is fixed to the top of the test frame (1) by several angle steels; the bottom plate (3) is fixed to the bottom of the test frame (1) by several angle steels.

9. A method for unwinding, utilizing the active unwinding device for observing freely moving animals as described in claim 1, characterized in that, The steps for unwinding the entangled / twisted connector between the head-mounted miniature fluorescence microscope for live animals and the data acquisition device are as follows: (a) Before the live animal moves freely, the connection is in a state of no force, i.e. no entanglement / twisting occurs, and the signal value of the sensing device is recorded as the initial signal value; (b) When the live animal moves freely, the live animal will entangle / twist with the connector, which will cause the sensor to rotate or generate torque, and the signal value of the sensor will change. (c) The sensing device converts the signal generated by the winding / twisting of the connector into an electrical signal and transmits it to the controller through the signal line; (d) The controller receives the signal and makes a judgment. When the real-time signal value of the sensing device deviates from the initial signal value, the controller controls the unwinding device to rotate and unwind in the same direction as the rotation direction of the live animal. (e) The sensing device periodically sends electrical signals to the controller to record the winding state of the connector; (f) When the signal value of the sensing device reaches the initial signal value, the winding / twisting is unwound, and the controller controls the unwinding device to stop rotating.

10. A method for unwinding the unwinding device according to claim 1, characterized in that, The steps are as follows: (a) Before the live animal moves freely, record the signal value of the sensing device (7) when the connector is not under force, i.e., without entanglement / twisting, and record it as the initial signal value; (b) When the live animal moves freely, the live animal will wrap around / twist the connector, and the connector will cause the sensing device (7) to rotate or generate torque on the connecting rod (10); (c) The sensing device (7) converts the signal generated by the winding / twisting on the connecting rod (10) into an electrical signal and transmits it to the controller (11) through the signal line of the conductive slip ring (8). (d) After receiving the signal, the controller (11) controls the stepper motor (4) to rotate in the opposite direction to the rotation direction of the live animal; (e) The stepper motor (4) drives the drive gear (52) to rotate in the opposite direction to the rotation direction of the live animal; the drive gear (52) drives the driven gear (51) to rotate in the same direction as the rotation direction of the live animal; (f) The driven gear (51) drives the data acquisition unit (6) and the sensing device (7) to rotate in the same direction as the rotation of the live animal, thereby beginning to untangle the tangled / twisted connection; (g) The sensing device (7) sends an electrical signal to the controller (11) in real time to record the winding state of the connector; (h) When the signal value of the sensing device (7) reaches the initial signal value, the winding / twisting is unwound, and the controller (11) controls the stepper motor (4) to stop rotating.

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