Therapeutic fusion proteins

Abstract

The present invention relates to fusion proteins suitable for use as drugs or research tools. The therapeutic uses of the fusion proteins may include preventing or treating acute or chronic inflammatory and immune system-driven organ and microvascular disorders, such as acute kidney injury, acute myocardial infarction, acute respiratory distress or chronic obstructive pulmonary disease fibrosis, and other organ damage caused by tissue trauma and acute and chronic injuries.

Description

[0001] Sequence Listing

[0002] This application contains a sequence listing that has been submitted electronically in ASCII format and is hereby incorporated by reference in its entirety. The ASCII copy was created on August 31, 2020, is named PAT058332_SL.txt and is 653,193 bytes in size. Technical Field

[0003] The present invention relates to a fusion protein comprising both integrin binding ability and phosphatidylserine binding ability. The fusion protein can be used as a therapeutic agent, in particular for preventing or treating acute or chronic inflammatory disorders and immune system driven or coagulation driven organ and microvascular disorders. Background Art

[0004] Acute inflammatory organ injury (AOI) has historically been a challenging disease with high morbidity, high mortality and significant unmet medical needs. Typical AOIs include myocardial infarction (MI) and stroke, which occur in 32.4 million patients worldwide each year. Patients with previous MI and stroke are considered by the World Health Organization to be at the highest risk of further coronary and cerebral events, which are one of the top causes of morbidity in developed countries. Another AOI is acute kidney injury (AKI), which occurs in approximately 13.3 million people each year. In high-income countries, the incidence of AKI is 3-5 / 1000 and is associated with a high mortality rate (14%-46%) (Metha et al., (2015) Lancet, 385(9987):2616-43). Similar to MI and stroke, AKI survivors often do not fully recover and are at increased risk of developing chronic kidney disease or end-stage renal disease. To date, there are no FDA-approved drugs to prevent or treat AKI. The development of new treatments for AKI has proven challenging, with clinical trials thus far without successful results. This is likely due to the multifactorial and multifaceted pathophysiology of AKI, which includes inflammation, microvascular dysfunction, and nephrotoxic pathologies caused by suppuration, ischemia / reperfusion, and / or nephrotoxic injury. These drivers can act simultaneously or sequentially, resulting in damage to a large portion of the renal tubule and glomerular cells, loss of renal functional reserve, and ultimately renal failure.

[0005] A common common feature of AOI is increased cell death due to tissue damage, increased production of cell debris, and pro-thrombotic / pro-inflammatory microparticles that can enter the circulation and injured tissues. After neutrophil tissue infiltration to resist infection, neutrophils undergo apoptosis or other forms of cell death in the affected tissue. Neutrophils contain harmful substances, including proteolytic enzymes and danger-associated molecular patterns (DAMPs), which can promote host tissue damage and propagate inflammation. Effective ingestion of dying cells triggers signaling events that cause macrophages (MΦ) to be reprogrammed to a non-inflammatory pro-resolving phenotype and release key mediators to successfully resolve and repair affected tissues. This reprogramming has recently been attributed to metabolic signaling, which activates phagocytic anti-inflammatory responses in macrophages (Zhang et al., (2019) Cell Metabolism, 29 (2): 443-56). Removal of debris or aging or dying cells in a non-inflammatory manner is called "efferocytosis".

[0006] However, in the case of delayed efferocytosis, necrotic cells accumulate and cause inflammatory responses, such as triggering proinflammatory cytokines (TNF-α) or immunosuppressive IL-10 by macrophages (Greenlee-Wacker (2016) Immunol. Reviews, 273: 357-370). In addition, if cell debris and microparticles are not effectively cleared, they can cause cell clumps and aggregations, such as neutrophil-platelet debris clusters, microthrombi and / or release of danger-associated molecular patterns (DAMPs), such as ATP, DNA, histones or HMGB1. The consequences may include obstruction of the microvasculature, dysfunction and significant sterile inflammation, leading to tissue damage, primary and secondary organ failure or the progression of adverse adaptive repair.

[0007] In the acute phase of AOI, the efferocytosis pathway appears to be significantly downregulated. Inflammation or acute injury responses (mechanical cues, hypoxia, oxidative stress, radiation, inflammation, and infection) can inhibit effective efferocytosis or phagocytosis by downregulating specialized phosphatidylserine (PS) binding proteins, which include bridging proteins and cell surface efferocytosis / scavenging receptors. An example of defunctionalization of efferocytosis receptors is the proteolytic shedding of TAM family receptors such as Mer tyrosine kinase (MerTK). MerTK is an integral membrane protein preferentially expressed on phagocytes, where it is both a signaling protein and can promote efferocytosis (through proteins such as Gas6 or protein S) and inhibit inflammatory signaling. The metalloproteinase ADAM17 induces proteolytic cleavage and release of the soluble extracellular domain of MerTK. The shedding process can reduce efferocytosis of phagocytes by depriving them of surface MerTK. In addition, the released extracellular domain can also inhibit efferocytosis in vitro (Zhang et al., (2015) J Mol Cell Cardiol. [Journal of Molecular and Cellular Cardiology], 87: 171-9; Miller et al., (2017) Clin Cancer Res. [Clinical Cancer Research], 23 (3): 623-629). Increased amounts of soluble Mer are typically observed in serum / plasma of inflammatory, malignant or autoimmune diseases (e.g., diabetic nephropathy or systemic lupus erythematosus (SLE)) and can mark disease severity (Ochodnicky P (2017) Am J Pathol. [American Journal of Pathology], 187 (9): 1971-1983; Wu et al., (2011) Arthritis Res Ther. [Arthritis Research and Treatment] 13: R88). In addition, bridging proteins such as milk fat globule-EGF factor 8 protein (MFG-E8) are also downregulated in most acute and chronic inflammatory diseases. Similar to soluble Mer, reduced serum / plasma concentrations of MFG-E8 can be found in patients with MI or stable angina (Dai et al., (2016) World J Cardiol., 8(1): 1-23) and mark the disease as described for chronic obstructive pulmonary disease (COPD; Zhang et al., (2015) supra).

[0008] Exposure of phosphatidylserine (PS) on dying cells is an evolutionarily conserved anti-inflammatory and immunosuppressive signal for immune cells. A large number of major mammalian pathogens utilize PS-mediated uptake as part of toxic cell infection (Birge et al., (2016) Cell Death Diff. [Cell Death and Differentiation], 23 (6): 962-78). For example, viruses can bind to PS binding receptors directly or via proteins such as Gas6 (Morizono and Chen (2014) J Virol. [Journal of Virology], 88 (8): 4275-90). Inactivation of endogenous clearance pathways in response to injury may represent an evolutionary response, thereby reducing the efficiency of infectious agents to enter and hijack cells after injury and thereby mask the host's immune response and defense. Therefore, downregulation of clearance pathways will increase the efficacy of innate and adaptive immune effectors to resist infection. As a result of "friendly fire", efferocytosis may be temporarily affected during acute organ injury, and the above-mentioned complications of AOI may occur. Accumulation of dying cells, debris, pro-inflammatory and pro-thrombotic MPs is a hallmark of AOI and a major contributor to inflammation and microvascular damage. Notably, this accumulation of pro-inflammatory and pro-thrombotic microparticles is common in serious diseases with high medical needs and may contribute to their morbidity. Examples of such indications are sepsis and cancer (Yang et al. (2016) Tumour Biol. 37(6):7881-91; Zhao et al. (2016) J Exp Clin Cancer Res. 35:54; Muhsin-Sharafaldine et al. (2017) Biochim Biophys Acta Gen Subj. 1861(2):286-295; Ma et al. (2017) Sci Rep. 7(1):4978; Souza et al. (2015) Kidney Int. 87(6):1100-8). Previous drug discovery efforts in this field have focused on PS binding proteins, which can be used as the basis for candidate drug design as described by (Li et al., (2013) Exp Opin Ther Targets, 17(11): 1275-1285).

[0009] A subset of PS binding proteins also recognize and bind to integrins, such as αvβ3 and αvβ5, which are expressed on many cell types, including phagocytes. These proteins act to bridge PS, exposing apoptotic / dying cells to integrins, leading to efferocytosis (also known as phagocytosis) by macrophages and non-professional phagocytes. Some bridging proteins are also downregulated in most acute and chronic inflammatory diseases. Therapeutic uses of this bridging protein or its truncated forms have been proposed previously (WO 2006122327 (sepsis), WO 2009064448 (organ damage after ischemia / reperfusion), WO 2012149254 (cerebral ischemia), The Feinstein Institute for Medical Research; WO 2015025959 (myocardial infarction) Kyushu University & Tokyo Medical University; WO 20150175512 (bone resorption) University of Pennsylvania; WO 2017018698 (tissue fibrosis) Korea University Research and Business Foundation and US 20180334486 (tissue fibrosis) Nexel Co., Ltd.); WO 2020084344; However, the use of wild-type or naturally occurring proteins is limited by many problems. For example, when cultured in a cell expression system, wild-type MFG-E8 (wtMFG-E8) is considered to have poor developability, low solubility and is expressed in very low yields. Work by Castellanos et al. (2016) showed that MFG-E8 expressed as an Fc-IgG fusion protein in insect or CHO cells completely aggregated and could only be effectively purified by adding detergents such as Triton X-100 or CHAPS (Castellanos et al., (2016) Protein Exp. Pur. [Protein Experiment and Purification], 124: 10-22).

[0010] The main functions of MFG-E8 reported to date are enhancement of efferocytosis (Hanayama 2004 Science), regulation of lipid uptake / processing (Nat Med. 2014). rMFG-E8 regulates enterocyte-specific lipid storage by promoting enterocyte triglyceride hydrolase (TG) activity (JCI 2016). Intracellular MFG-E8 has been shown to be a suppressor of hepatic lipid accumulation and inflammation, acting by inhibiting the ASK1-JNK / p38 signaling cascade. (Zhang et al. 2020). In addition, anti-inflammatory properties, promotion of angiogenesis, atherosclerosis, tissue remodeling, and hemostatic regulation have been described for MFG-E8. In addition, MFG-E8 has been reported to remove excess collagen in lung tissue by binding to collagen through its C1 domain. Interestingly, MFG-E8- / - macrophages exhibit a defect in collagen uptake, which can be rescued by recombinant MFG-E8 containing at least one Dictyostelium lectin domain (Atabai et al. 2009).

[0011] In preclinical studies, recombinant MFG-E8 has shown convincing protective effects in various (mainly) rodent models of acute inflammation and organ disease, as well as in disease models of healing abnormalities. Recombinant MFG-E8 has been shown to accelerate wound healing in diabetic and I / R-induced wounds / ulcers (Uchiyama et al. 2015 / 2017); accelerated repair of the intestinal epithelium after colitis (Bu et al. 2007) and accelerated tendon repair after injury (Shi et al. 2019); recombinant MFG-E8 reduced renal damage and fibrosis in the ureteral obstruction (UUO) model (Brisette et al. 2016). In addition, efficacy has been demonstrated in typical fibrosis models, where recombinant MFG-E8 accelerated the regression of TAA- and CCl4-induced liver fibrosis (An SY, Gastroenterology 2016) and protected in a bleomycin-induced lung fibrosis model (Atabai et al. 2009). Recently, a truncated version of C2 depletion was published to exert similar or even better efficacy in several preclinical fibrosis models, including the TAA liver fibrosis model (WO 2020084344).

[0012] EDIL3 (EGF-like repeats and Dictyostelium I-like domain-containing protein 3) was recently reviewed by Hajishengallis and Chavakis 2019. EDIL3 (alias DEL-1) has been shown to mediate efferocytosis, regulate neutrophil recruitment and inflammation, can be triggered as part of emergency myelopoiesis (ɑvb3-integrin-dependent) in the hematopoietic stem cell niche, limit osteoclastogenesis and inhibit inflammatory bone loss in rodents and non-human primates. EDIL3 was found to be an integral component of central nervous system immune privilege. The potential of EDIL3 as a therapeutic protein has been tested as a fusion protein with a human IgG Fc fragment (DEL-1-Fc). In a mouse model of periodontitis, administration of DEL-Fc inhibited neutrophil infiltration and blocked IL-17-driven inflammatory bone loss (Eskan et al. 2012 doi:10.1038 / ni.2260). Additionally, in a non-human primate periodontitis model, DEL-1-Fc improved periodontal inflammation, tissue destruction, and bone loss (Shin et al. 2015 DOI: 10.1126 / scitranslmed.aac5380). In addition, DEL-1-Fc improved relapsing-remitting experimental autoimmune encephalomyelitis (EAE), a translational multiple sclerosis model (Choi et al. 2014 doi: 10.1038 / mp.2014.146); in addition, DEL-1-Fc also reduced the incidence and severity of postoperative abdominal adhesions in a mouse model (Fu et al. 2018).

[0013] Removal of dying cells, debris, and microparticles by bridging proteins (e.g., MFG-E8, EDIL3, Gas6) can eliminate the primary cause of sterile inflammation and microvascular dysfunction and thereby prevent the progression of tissue damage and enable resolution of inflammation. Thus, therapeutic approaches that promote clearance of dying cells during AOI can be used to reduce or at least alleviate the pathology of AOI and may be of interest in other diseases where clearance of dying cells or microparticles exposed to PS is inadequate. Thus, there is a need for therapeutic agents that can be used to reduce tissue damage and inflammation and have desirable manufacturing properties to address unmet medical needs in AOI. Summary of the invention

[0014] In the present disclosure, applicants have produced recombinant therapeutic fusion proteins based on the structure of naturally occurring bridging proteins (e.g., MFG-E8) without the above-mentioned undesirable properties and production problems of wild-type proteins. The fusion proteins of the present disclosure comprise an integrin binding domain (e.g., an EGF-like domain), a solubilizing domain, and a phosphatidylserine binding domain (e.g., the C1 domain from MFG-E-8 or its paralog EDIL3). The proteins of the present invention are suitable for preventing or treating acute or chronic inflammatory, immune system-driven, or fibrosis-driven organ disorders. The proteins of the present invention may also find applications in enabling, accelerating, and promoting repair and regeneration.

[0015] The fusion protein maintains the main biological function of the wild-type MFG-E8 or EDIL3 protein, for example, by acting to bridge dead cells, debris and particles exposed to PS to phagocytes and thereby triggering efferocytosis. In addition, compared with the wild-type MFG-E8 protein (SEQ ID NO: 1) or with recombinant MFG-E8 and C2 truncated MFG-E8 (EGF_C1), the therapeutic fusion protein disclosed herein has improved developability, particularly reduced viscosity and improved solubility. In addition, compared with the wild-type MFG-E8 protein, these therapeutic fusion proteins have longer plasma exposure and higher yield when expressed in a cell expression system. The therapeutic fusion protein according to the present invention has increased macrophage selective activity (enhanced efferocytosis). In addition, compared with the full-length wild-type MFG-E8 or other full-length functional variants, the therapeutic fusion protein according to the present invention has higher safety.

[0016] Provided herein are therapeutic fusion proteins for enhancing efferocytosis, comprising an integrin binding domain, a phosphatidylserine (PS) binding domain, and a solubilization domain, wherein the PS binding domain is a truncated variant of at least one PS binding domain listed in Table 2.

[0017] In some embodiments, the therapeutic fusion protein comprises the C-terminus of an integrin binding domain linked to the N-terminus of a solubilizing domain and the C-terminus of a solubilizing domain linked to a PS binding domain. In some embodiments, the therapeutic fusion protein comprises the general structure EGF-SC, wherein EGF represents an integrin binding domain, such as an EGF-like domain of MFG-E8, EDIL3, or any other protein comprising an integrin binding domain listed in Table 1; S represents a solubilizing domain; and C represents a truncated PS binding domain, such as a truncated variant of a PS binding domain found in any of MFG-E8, EDIL3, or other proteins comprising C1 and / or C2 of a PS binding domain listed in Table 2. Table 3 lists examples of proteins comprising both an integrin binding domain and a PS binding domain, such as MFG-E8 (SEQ ID NO: 1) and EDIL3 (SEQ ID NO: 11).

[0018] In some embodiments, the PS binding domain comprises one of the two Dictyostelium C1-C2 subdomains or a functional variant thereof. In some embodiments, the PS binding domain of human MFG-E8 has an amino acid sequence as shown in SEQ ID NO: 3 or an amino acid sequence as shown in SEQ ID NO: 3 having at least 90%, 95%, 96%, 97%, 98% or 99% identity or a truncated variant thereof. In one embodiment, the truncated PS binding domain comprises a truncated PS binding domain of human MFG-E8 or a functional variant thereof, and the functional variant comprises one, two, three, four, five, or up to 10 amino acid modifications. In one embodiment, the PS binding domain comprises a truncated PS binding domain of human EDIL3 or a functional variant thereof, and the functional variant comprises one, two, three, four, five, or up to 10 amino acid modifications.

[0019] In certain aspects, the present invention provides a fusion protein comprising an epidermal growth factor (EGF)-like domain, a solubilization domain, a C1 domain, but lacking a functional C2 domain. In some embodiments, the fusion protein comprises an epidermal growth factor (EGF)-like domain, a solubilization domain, a C1 domain, but lacking a medin polypeptide or a fragment thereof.

[0020] In some embodiments, the solubilization domain of the fusion protein is connected to the integrin binding domain. In some embodiments, the solubilization domain is connected to the PS binding domain. In some embodiments, the solubilization domain is connected to both the integrin binding domain and the PS binding domain, i.e., is located between the integrin binding domain and the PS binding domain. In some embodiments, the solubilization domain is inserted into the integrin binding domain or inserted into the PS binding domain. In one embodiment, the therapeutic fusion protein has a structure, and the structure from N-terminus to C-terminus is: integrin binding domain-solubilization domain-PS binding domain.

[0021] In some embodiments, the integrin binding domain of the therapeutic fusion protein comprises an arginine-glycine-aspartic acid (RGD) binding motif and binds to αvβ3 and / or αvβ5 or α8β1 integrin.

[0022] In some embodiments, the solubilization domain of the therapeutic fusion protein is directly linked to the integrin binding domain and / or to the PS binding domain, i.e., inserted between the domains. In alternative embodiments, the solubilization domain is indirectly linked to the integrin binding domain and / or the PS binding domain via a linker, e.g., an external linker. In some embodiments, the solubilization domain comprises human serum albumin (HSA), domain 3 of HSA (HSA D3), or the Fc region of IgG (Fc-IgG) or a functional variant thereof.

[0023] In some embodiments, the integrin binding domain is an EGF-like domain (e.g., an EGF-like domain having an amino acid sequence as shown in SEQ ID NO: 2 or an amino acid sequence having at least 90%, 95%, 96%, 97%, 98% or 99% identity with the amino acid sequence as shown in SEQ ID NO: 2) or a truncated variant thereof. In one embodiment, the EGF-like domain comprises an EGF-like domain of human MFG-E8 or a functional variant thereof, wherein the functional variant comprises one, two, three, four, five, or up to 10 amino acid modifications. In one embodiment, the EGF-like domain comprises an EGF-like domain of human EDIL3 or a functional variant thereof, wherein the functional variant comprises one, two, three, four, five, or up to 10 amino acid modifications.

[0024] In some embodiments, the solubilizing domain is HSA or a functional variant thereof (e.g., an amino acid sequence having at least 90%, 95%, 96%, 97%, 98% or 99% sequence identity with the amino acid sequence shown in SEQ ID NO: 4) or a truncated variant thereof. In one embodiment, HSA comprises an amino acid substitution C34S (which has the function of reducing the tendency of protein aggregation) and has an amino acid sequence shown in SEQ ID NO: 5. In some embodiments, the solubilizing domain comprises human serum albumin (HSA) or a functional variant thereof (the functional variant comprises one, two, three, four, five, up to 10 amino acid modifications, such as HSA C34S) or a truncated variant of HSA (e.g., domain 3 of HSA (HSA D3)) or a functional variant of the truncated variant. In a preferred embodiment, the solubilizing domain is HSA C34S.

[0025] In alternative embodiments, the solubilization domain comprises an Fc region of IgG (Fc-IgG) (e.g., an Fc region of human IgG1, IgG2, IgG3, or IgG4) or a functional variant thereof. In one embodiment, the solubilization domain comprises an Fc region of human Fc-IgG1 (having an amino acid sequence as shown in SEQ ID NO: 7 or an amino acid having at least 90%, 95%, 96%, 97%, 98%, or 99% sequence identity with an amino acid sequence as shown in SEQ ID NO: 7) or a truncated variant thereof. In one embodiment, Fc-IgG1 comprises amino acid substitutions D265A and P329A to reduce Fc effector function and has an amino acid sequence as shown in SEQ ID NO: 8. In another embodiment, Fc-IgG1 comprises an amino acid substitution T366W to produce a "knob", or it may comprise amino acid substitutions T366S, L368A, Y407V to produce a "hole". In addition, the Fc-IgG1 knob may comprise an amino acid substitution S354C, and the Fc-IgG1 hole may comprise an amino acid substitution Y349C, thereby forming a cysteine ​​bridge when paired. In addition to the knob and hole modifications, Fc-IgG1 may also comprise D265A and P329A substitutions to reduce Fc effector function. In one embodiment, Fc-IgG1 has an amino acid sequence as shown in SEQ ID NO: 9 or 10.

[0026] In a preferred embodiment, the therapeutic fusion protein comprises a milk fat globule-EGF factor 8 protein (MFG-E8) and a solubilization domain, wherein MFG-E8 comprises a functional variant of an integrin-binding EGF-like domain (SEQ ID NO: 2) and a phosphatidylserine-binding C1-C2 domain (SEQ ID NO: 3 or SEQ ID NO: 76). MFG-E8 may comprise naturally occurring or wild-type human MFG-E8 (SEQ ID NO: 1), or MFGE-8 having SEQ ID NO: 75, or a functional variant thereof. In one embodiment, the solubilization domain is linked to the N or C terminus of MFG-E8. In one embodiment, the solubilization domain is inserted between the EGF-like domain and the C1 domain or between the EGF-like domain and the C2 domain. In a preferred embodiment, the solubilization domain is linked to the C-terminus of the EGF-like domain and to the N-terminus of the C1 domain. The solubilization domain may be directly or indirectly linked to the C-terminus of the EGF-like domain and directly or indirectly linked to the N-terminus of the C1 domain. In some embodiments, the indirect attachment is via an external linker, such as a glycine-serine based linker.

[0027] In some embodiments, and as described in the Examples section, the function of the therapeutic fusion protein of the present disclosure is to promote endothelial cell efferocytosis in a human endothelial cell-Jurkat cell efferocytosis assay, and to restore impaired efferocytosis of macrophages and enhance basal efferocytosis in a human macrophage-neutrophil efferocytosis assay; the function of the fusion protein is to reduce the number of plasma microparticles by clearance in a human endothelial cell microparticle efferocytosis assay; and / or the fusion protein provides protection against multi-organ injury in an acute renal ischemia model.

[0028] The present invention also discloses methods, uses, diagnostic reagents, pharmaceutical compositions and kits using or containing these therapeutic fusion proteins. The present invention also provides nucleic acids encoding the disclosed fusion proteins, clones and expression vectors containing such nucleic acids, host cells containing such nucleic acids, and methods for producing the disclosed fusion proteins by culturing such host cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 Schematic diagrams of examples of therapeutic fusion proteins of the present disclosure are shown. A solubilization domain (labeled 'SD') is linked to the C-terminus, N-terminus, or between the EGF, C1 or C2 domains of MFG-E8.

[0030] Figure 2 Shown are a number of SDS-PAGE protein gels of fusion proteins expressed in HEK cells. Figure 2A: EGF-HSA-C1-C2 protein (FP330; SEQ ID NO: 42); Figure 2 B: EGF-HSA-C1-C2 of EDIL3 protein (FP050; SEQ ID NO: 12); Figure 2 C: Unreduced and reduced EGF-Fc (KiH) C1-C2 protein (this protein is a heterodimer of FP071 (EGF-Fc (knob) -C1-C2; SEQ ID NO: 18) and Fc-IgG1 hole (SEQ ID NO: 10); Figure 2 D: EGF-HSA-C1 protein (FP260; SEQ ID NO: 34). Figure 2 In each of A, 2C and 2D, the first column shows the standard markers without staining of the Precision Plus protein and the second column shows the respective fusion protein. Figure 2 B, The first column shows the fusion protein and the second column shows the Precision Plus protein unstained standard marker. Figure 2 E shows other recombinant proteins that have been produced and purified.

[0031] Figure 3 The effect of loss of wild-type (wt) MFG-E8 protein compared to the fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) during actual manipulation is illustrated. Figure 3 A shows the loss of efficacy of wtMFG-E8 in the L-α-phosphatidylserine competition assay when protein dilutions were prepared in polypropylene plates (symbols: □) compared to dilutions prepared in non-binding plates (symbols: ●). Figure 3 B shows that in the PS competition assay, the fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) showed little loss in efficacy when protein dilutions were prepared in polypropylene plates (symbols: □) compared to non-binding plates (symbols: ●).

[0032] Figure 4 The binding of the fusion protein to L-α-phosphatidylserine is shown. Figure 4 A shows the binding of FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) to immobilized L-α-phosphatidylserine and to a lesser extent the phospholipid cardiolipin in a concentration-dependent manner. Figure 4B shows the binding of human wtMFG-E8 and a number of therapeutic fusion proteins to immobilized L-α-phosphatidylserine in a competition assay format (competition of biotinylated mouse wtMFG-E8 binding to L-α-phosphatidylserine) in a concentration-dependent manner: FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44), FP250 (EGF-HSA; SEQ ID NO:32), FP260 (EGF-HSA-C1; SEQ ID NO:34) and FP270 (EGF-HSA-C2; SEQ ID NO:36).

[0033] Figure 5 αv-integrin-dependent cell adhesion to the fusion protein was demonstrated. Figure 5 A shows that the αv integrin inhibitor cilengitide or 10 mM EDTA completely blocked cell adhesion to FP330 (EGF-HSA-C1-C2; SEQ ID NO: 42). Figure 5 As shown in B, a single point mutation in the integrin binding motif RGD (RGD>RGE) of the EGF-like domain (FP280; SEQ ID NO:38) resulted in complete abrogation of cell adhesion. Figure 5 C shows that despite the presence of the EGF-like domain, the immobilized EGF-HSA protein (FP250; SEQ ID NO: 32) did not support or only moderately supported the adhesion of BW5147.G.1.4 cells. Figure 5 As shown in D, when expressed in CHO cells or HEK cells, the fusion protein of the present disclosure (FP330; SEQ ID NO: 42) promoted αv-integrin-dependent cell adhesion similar to wtMFG-E8.

[0034] Figure 6 The effect of the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) in promoting efferocytosis of dying neutrophils by human macrophages is shown. The concentration of the fusion protein is shown on the x-axis, and efferocytosis [%] is shown on the y-axis.

[0035] Figure 7 It was shown that the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) can rescue the efferocytosis of dying neutrophils by human macrophages that is impaired by endotoxin (lipopolysaccharide). Figure 7A shows efferocytosis of dying human neutrophils by impaired macrophages at 100 pg / ml of lipopolysaccharide (LPS) in three human donors. The left panel shows the response of a single donor, and the right panel shows the average efferocytosis (%) of the three donors. Figure 7 B shows the rescue of this endotoxin (LPS)-impaired efferocytosis of dying neutrophils by human macrophages in the presence of the therapeutic fusion protein FP278. Efferocytosis indices from three different human macrophage donors were normalized and plotted as efferocytosis (%).

[0036] Figure 8 Shown is the rescue of S. aureus particle-induced impairment of human macrophage efferocytosis of dying neutrophils by the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44). Figure 8 A shows the effect of FP278 at a concentration of 100 nM in promoting efferocytosis above basal levels (dashed line; left-hand portion of the graph), and the effect of FP278 at 100 nM in rescuing the impairment of efferocytosis caused by administration of S. aureus (right-hand portion of the graph). Figure 8 B shows the increase of fusion protein FP278 (EC 50 8 nM) to rescue the impaired efferocytosis caused by administration of S. aureus, as well as to promote efferocytosis once the basal level of efferocytosis was reached.

[0037] Fig. 9 The effect of the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) in promoting efferocytosis of dying Jurkat cells by human endothelial cells (HUVEC) is shown. The efficiency of the fusion protein in the endothelial cell efferocytosis assay depends on the presence of the C1-C2 or C1-C1 tandem domains, because Fig. 9 As shown, a fusion protein with the structure EGF-HSA-C2 (FP270; SEQ ID NO: 36) was ineffective in this assay.

[0038] Fig.10 Shown is that the position of the HSA domain in the therapeutic fusion protein, i.e., at the N- or C-terminal position (FP220 (HSA-EGF-C1-C2; SEQ ID NO:30) or FP110 (EGF-C1-C2-HSA; SEQ ID NO:28), respectively), confers efferocytosis blocking function to the MFG-E8 HSA fusion protein in a macrophage efferocytosis assay. The concentration of the fusion protein is shown on the x-axis and efferocytosis [%] is shown on the y-axis.

[0039] Fig.11 A comparison of the promotion of efferocytosis by various forms of therapeutic fusion proteins comprising HSA or Fc portions is shown. The concentration of fusion protein (nM) is shown on the x-axis and the efferocytosis effect [MFI] is shown on the y-axis. Fig.11 A shows a comparison of fusion proteins comprising HSA (wherein HSA is located at the C-terminus or N-terminus or between the EGF-like domain and the C1 domain); FP110 (EGF-C1-C2-HSA; SEQ ID NO:28), FP220 (HSA-EGF-C1-C2; SEQ ID NO:30) and FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44), respectively. Fig.11 B shows a comparison of fusion proteins comprising an Fc portion, wherein the Fc is located at the C-terminus (FP060 (EGF-C1-C2-Fc [S354C, T366W]; SEQ ID NO: 14) and FP080 (EGF-C1-C2-Fc; SEQ ID NO: 22)) or located between the EGF-like domain and the C1 domain (FP070 (EGF-Fc-C1-C2; SEQ ID NO: 16)), compared to wild-type MFG-EG (SEQ ID NO: 1). Two forms of the Fc portion are shown: wild-type Fc (FP080; SEQ ID NO: 22) and an Fc portion with S354C and T366W modifications (EU numbering; FP060; SEQ ID NO: 14). Fig.11 C shows a comparison of three batches of the fusion protein FP090 (Fc-EGF-C1-C2; SEQ ID NO: 24) comprising an Fc portion at the N-terminus at three different concentrations (0.72, 7.2 and 72 nM) compared to a wt-MFG-E8 control. Fig.11 D shows promotion of efferocytosis by the fusion protein construct FP050 comprising HSA inserted between the EGF-like domain and the C1-C2 domain of EDIL3 (EDIL3-based EGF-HSA-C1-C2; SEQ ID NO: 12). Fig.11E shows other examples of fusion proteins of the present disclosure, such as chimeric variants (FP114 or FP260; SEQ ID NO: 34, FP147 or FP1777; SEQ ID NO: 71, FP1149, FP1150, FP145; SEQ ID NO: 80, FP1145; SEQ ID NO: 103, FP146; SEQ ID NO: 82, FP1146) and a combination of the integrin binding domain of MFGE8 or EDIL3 with a PS binding domain such as the IgSF V domain of TIM4 or the GLA domain of the bridging protein GAS6 (FP1147 and FP1148).

[0040] Fig.12 The promotion of efferocytosis of HUVEC cells by the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) tested at 3 different concentrations (up to 30 nM) is shown. The promotion of efferocytosis is concentration-dependent, wherein efferocytosis increases with increasing concentrations of the fusion protein FP278.

[0041] Fig.13 The therapeutic fusion protein FP330 (EGF-HSA-C1-C2; SEQ ID NO: 42; Fig.13 A), FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44; Fig.13 B) and FP776 (EGF-HSA-C1-C2; SEQ ID NO: 48; Fig.13 C) Human macrophages can rescue the efferocytosis of dying neutrophils impaired by endotoxin (lipopolysaccharide). The concentration of the fusion protein is shown on the x-axis, and the efferocytosis [%] is shown on the y-axis.

[0042] Fig.14 The fusion protein FP330 (EGF-HSA-C1-C2; SEQ ID NO: 42; Fig.14 A), FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44; Fig.14 B) and FP776 (EGF-HSA-C1-C2; SEQ ID NO: 48; Fig.14 C) Effect on promoting efferocytosis of dying Jurkat cells by human endothelial cells (HUVEC). The concentration of fusion protein is shown on the x-axis and efferocytosis [%] is shown on the y-axis.

[0043] Fig.15A single dose of the therapeutic fusion proteins FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44), FP330 (EGF-HSA-C1-C2; SEQ ID NO:42), or FP776 (EGF-HSA-C1-C2; SEQ ID NO:48) was shown to protect renal function in an ischemia-reperfusion injury-induced acute kidney injury (AKI) model. Fig.15 A shows that the increase in serum creatinine (sCr) (mg / dL; y-axis) was reduced by intraperitoneal (ip) administration of 0.16 mg / kg or 0.5 mg / kg of FP278 (SEQ ID NO: 44) (x-axis). Fig.15 As shown in B, intravenous (iv) administration of 0.5 mg / kg or 1.5 mg / kg of fusion protein FP330 (SEQ ID NO: 42) significantly reduced serum creatinine levels. Fig.15 C shows that iv administration of fusion protein FP776 (SEQ ID NO: 48) lowered serum creatinine in a dose-dependent manner.

[0044] Fig.16 It was shown that a single dose of 0.16 mg / kg or 0.5 mg / kg of the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44) reduced blood urea nitrogen (BUN) levels in a murine model of acute kidney injury.

[0045] Fig.17 It was shown that a single dose of the therapeutic fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44) can protect distant organs from the acute phase response caused by ischemia-reperfusion-induced AKI based on gene expression of injury markers. Fig.17 A illustrates this AKI-induced serum amyloid (SAA) response in the mouse heart, Fig.17 B illustrates this AKI-induced response (SAA) in murine lung, both of which were potently blocked by a single intraperitoneal injection of 0.16 mg / kg or 0.5 mg / kg / ip of the MFG-E8-derived fusion protein FP278 (SEQ ID NO:44).

[0046] Fig.18 The results show that the liver responds to superparamagnetic iron oxide (SPIO) contrast agents over time. (24 h after disease induction) or after sham surgery (24 h after nephrectomy) The bolus was injected intravenously for 1.2s into animals with AKI. Animals with AKI showed a significant reduction in the uptake of contrast media by the liver compared to sham-operated animals (target = Kupffer cells). Treatment (prophylactic administration at -30 minutes before AKI induction or therapeutic administration of fusion protein FP776 (EGF-HSA-C1-C2; SEQ ID NO: 48) at +5 hours after ischemia-reperfusion injury induction) protected AKI mice from loss of contrast media accumulation in the liver.

[0047] Fig.19 The therapeutic fusion protein FP114, also referred to herein as FP260 (EGF-HSA-C1 SEQ ID No: 34), was tested in the AKI model at 1.5 mg / kg / iv as described in the Examples. For this study, FP114 was administered 30 minutes before the onset of ischemia-reperfusion injury. Serum markers and kidney weights were assessed 24 hours after disease induction. Decreased serum creatinine and BUN and normal kidney weights indicate protection against AKI in this model.

[0048] Fig. 20 The therapeutic fusion protein FP135, also referred to herein as FP261 (EGF-HSA-C1 SEQ ID No: 73), was tested at 0.8 mg / kg / ip in the CCL4 fibrosis model. Treatment was started 4 weeks after fibrosis induction (with CCL4) (11 doses in total) or 5 weeks after fibrosis induction (with CCL4) (8 doses in total) (where 3 weekly doses were administered). Six weeks after the cessation of disease induction, CCL4 was administered to the third group of animals (4 doses in total). In all groups, FP135 was administered once a day for the last three days. Liver stiffness was assessed at baseline (at the beginning of the experiment) when CCL4 was stopped and 3 days after CCL4 was stopped. The data show that in animals treated with FP135 (starting after the 4th and 5th weeks of CCl4), accelerated regression of liver stiffness induced by CCL4 can be achieved.

[0049] Figure 21. Fig.21A Therapeutic fusion protein FP135 (EGF-HSA-C1 SEQ ID No: 73) was tested at 0.8 mg / kg / ip in the CCL4 fibrosis model. Treatment was started after 4 weeks of fibrosis induction (with CCL4) (11 doses in total) or after 5 weeks of fibrosis induction (with CCL4) (8 doses in total) (where 3 weekly doses were administered) or after 6 weeks of disease induction with CCL4 (4 doses in total). In all groups, FP135 was administered once a day in the last three days. The reduction in serum ALT showed that in the groups treated at the 4th and 5th weeks of CCl4, FP135 treatment helped to accelerate the regression of liver damage caused by CCL4.

[0050] Fig. 21B The therapeutic fusion protein FP135 (EGF-HSA-C1 SEQ ID No: 73) was tested in the CCL4 fibrosis model at 0.8 mg / kg / ip. Fig.21A The collagen content in the liver of the sacrificed animals was quantified by hydroxyproline assay. The reduction observed in the 8- and 11-fold dosed animals suggests that FP135 treatment helps accelerate the regression of CCL4-induced liver fibrosis.

[0051] Fig. 21C The therapeutic fusion protein FP135 (EGF-HSA-C1 SEQ ID No: 73) was tested in the CCL4 fibrosis model at 0.8 mg / kg / ip. Fig.21A Collagen expression in the liver of sacrificed animals was quantified by hydroxyproline assay. The reduction observed in animals treated with 8- and 11-fold doses suggests that treatment with FP135 helps accelerate the regression of CCL4-induced liver fibrosis.

[0052] Fig. 22 Integrin adhesion data for portions of the truncated proteins FP137, FP135 and FP147 are shown.

[0053] Fig.23 Dynamic light scattering (DLS) of C2 truncated MFG-E8 (EGF-C1; SEQ ID NO: 115) and HSA fusion (EGF-HSA-C1; SEQ ID NO: 73) are shown. DETAILED DESCRIPTION

[0054] Disclosed herein are therapeutic fusion proteins comprising an integrin binding domain, a PS binding domain, and a solubilization domain. Also disclosed herein are therapeutic methods using the disclosed fusion proteins and assays useful for characterizing the fusion proteins, such as efferocytosis assays.

[0055] definition

[0056] In order to make the present disclosure more easily understood, certain terms are specifically defined throughout the detailed description. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by ordinary technicians in the field to which the present disclosure belongs.

[0057] In all cases where the terms 'comprise, comprises, comprising', etc. are used in reference to a sequence (e.g., an amino acid sequence), it is understood that the sequence may also be qualified by the terms 'consist, consists, consisting', etc. As used herein, the phrase 'consisting essentially of refers to the genus or species of active agents included in a method or composition and any excipients that are inactive for the intended purpose of the method or composition. In some aspects, the phrase 'consisting essentially of explicitly excludes the inclusion of one or more additional active agents other than the multispecific binding molecules of the disclosure. In some aspects, the phrase 'consisting essentially of explicitly excludes the inclusion of one or more additional active agents other than the multispecific binding molecules of the disclosure and the second co-administered agent.

[0058] As used herein, the term "efferocytosis" refers to a process in cell biology in which dying or dead cells, such as apoptotic or necrotic or senescent cells or highly activated cells or extracellular vesicles (microparticles) or cell fragments - collectively referred to as "prey" - are removed by phagocytosis, i.e., engulfed and digested by phagocytic cells. During efferocytosis, phagocytes actively tether and engulf prey, generating large intracellular fluid-filled vesicles containing the prey, called efferosomes, generating lysosomal compartments in which degradation of the prey is initiated. During apoptosis, efferocytosis ensures that dying cells are removed before their membrane integrity is compromised and their contents may leak into surrounding tissues, thereby preventing exposure of surrounding tissues to DAMPs (e.g., toxic enzymes, oxidants, and other intracellular components, such as DNA, histones, and proteases). Obligate phagocytes include cells of myeloid origin, such as macrophages and dendritic cells, but others, such as stromal cells, can also undergo efferocytosis, such as epithelial and endothelial cells and fibroblasts. Impaired efferocytosis has been linked to autoimmune diseases and tissue damage and has been demonstrated in diseases such as cystic fibrosis, bronchiectasis, COPD, asthma, idiopathic pulmonary fibrosis, rheumatoid arthritis, systemic lupus erythematosus, glomerulonephritis, and atherosclerosis (Vandivier RW et al. (2006) Chest, 129(6):1673-82). To date, no therapy specifically designed to promote efferocytosis has entered the clinic.

[0059] The term 'efferocytosis assay' as used herein and as described in the Examples relates to a test system developed for the profiling of fusion proteins, which utilizes human macrophages or human endothelial cells (HUVEC) as phagocytic cells. Exemplified herein are macrophage-neutrophil efferocytosis assays, endothelial cell-Jurkat cell efferocytosis assays, or endothelial cell microparticle efferocytosis assays. As described in more detail in the Examples, these assays can be used to demonstrate that MFG-E8-derived biotherapeutics, such as the fusion proteins of the present disclosure, effectively promote efferocytosis of dying cells and microparticles by macrophages or endothelial cells. In addition, the described macrophage-neutrophil assays are suitable for demonstrating that such compounds of the present invention can even rescue efferocytosis of LPS or S. aureus damage to dying cells.

[0060] The terms 'polypeptide' and 'protein' are used interchangeably herein to refer to a polymer of amino acid residues. The phrase also applies to amino acid polymers in which one or more amino acid residues are artificial chemical mimetics of the corresponding naturally occurring amino acids, and to naturally occurring amino acid polymers and non-naturally occurring amino acid polymers. Unless otherwise indicated, a particular polypeptide sequence also implicitly encompasses conservatively modified variants thereof.

[0061] The term 'stickiness' as used herein with respect to the proteins of the present disclosure refers to the result of protein misfolding which promotes protein coagulation or aggregation. These undesirable and non-functional effects are the result of surface hydrophobic interactions.

[0062] As used herein, 'C-terminus' refers to the carboxyl terminal amino acid of a polypeptide chain having a free carboxyl group (-COOH). As used herein, 'N-terminus' refers to the amino terminal amino acid of a polypeptide chain having a free amine group (-NH2).

[0063] As used herein, the term 'fusion protein' refers to a protein comprising multiple domains, which may not constitute a complete natural or wild-type protein, but may be limited to the active domain of the complete protein responsible for binding to the corresponding receptor on the cell surface. Fusion proteins can be generated using recombinant protein design, wherein the term 'recombinant protein' refers to a protein that has been prepared, expressed, created or separated by recombinant DNA technology means. For example, tandem fusion refers to a technique in which the domain of a target protein or target protein is simply connected end-to-end by the fusion of the N or C termini between proteins. This provides a flexible bridge structure, thereby leaving enough space between fusion partners to ensure correct folding. However, the N or C termini of a peptide are usually key components for obtaining the desired folding pattern of a recombinant protein, with the result that a simple end-to-end connection of a domain may be invalid. Alternatively, the process of domain insertion involves fusing continuous protein domains by encoding the desired structure into a single polypeptide chain and sometimes inserting the domain into another domain. In both of the above processes, the domains are 'directly connected' or 'directly connected'. Due to the difficulty in finding a suitable connection site in the target gene, domain insertion is usually more difficult than tandem fusion.

[0064] In addition to the above-mentioned direct connection fusion technology, external joints can be used to maintain the function of the protein domain in the fusion protein. Such joints refer to a stretch of amino acids that connects a protein domain to another protein domain, and are referred to as "indirect joints" in this article. Therefore, these domains are "indirectly connected" or "indirectly connected". For example, it is understood by those of ordinary skill in the art that a polypeptide having a structure including two or more functional or organizational domains generally includes a stretch of amino acids that connect them to each other between these domains. Joints allow domains to interact, enhance stability and can reduce steric hindrance, even if N and C-termini can be fused, which often makes them preferred for use in engineered protein design. In certain embodiments, the joint is characterized in that it tends not to adopt a rigid three-dimensional structure, but to provide flexibility for the polypeptide. Various types of naturally occurring joints have been used in engineered proteins, such as immunoglobulin hinge regions, which are used as joints in many recombinant therapeutic proteins, particularly in engineered antibody constructs (Pack P et al., (1995) J.Mol.Biol. [Molecular Biology], 246: 28-34). In addition to natural linkers, many artificial linkers have been designed, which can be subdivided into three categories: flexible, rigid, and in vivo cleavable linkers. (Yu K et al., (2015) Biotech. Advances, 33(1):155-64; Chen X et al., (2013) Ad. Drug Delivery Reviews, 65(10):1357-69). The most widely used flexible linker sequences are (Gly)n (Sabourin et al., (2007) Yeast, 24:39-45) and (Gly 4 Ser)n (SEQ ID NO:64) (Huston et al., 1988, 85:5879-83), wherein the length of the linker can be adjusted by the copy number n. In some embodiments, the polypeptide comprising the linker element has an overall structure of the overall form D1-linker-D2, wherein D1 and D2 can be the same or different and represent two domains associated with each other by a linker. In some embodiments, the length of the polypeptide linker is at least 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 35, 40, 45, 50, 55, 60, 65, 70, 75, 80, 85, 90, 95, 100 or more amino acids.

[0065] As used herein, a 'modification' or 'mutation' of an amino acid residue / position refers to a change in the primary amino acid sequence compared to the starting amino acid sequence, wherein the change is caused by a sequence change involving the amino acid residue / position. For example, typical modifications include substitution of a residue (or substitution at the position) with another amino acid (e.g., conservative or non-conservative substitution), insertion of one or more amino acids near the residue / position, and deletion of the residue / position. An amino acid 'substitution' or a variation thereof refers to replacement of an existing amino acid residue in a predetermined (starting) amino acid sequence with a different amino acid residue. Typically and preferably, the modification alters at least one physicobiochemical activity of the variant polypeptide compared to a polypeptide comprising the starting (or 'wild-type') amino acid sequence.

[0066] The term 'conservatively modified variant' is applicable to both amino acids and nucleic acid sequences. For a particular nucleic acid sequence, conservatively modified variants refer to those nucleic acids encoding the same or substantially the same amino acid sequence, or, in the case where the nucleic acid does not encode an amino acid sequence, to substantially the same sequence. Due to the degeneracy of the genetic code, a large number of functionally identical nucleic acids encode any given protein. For example, codons GCA, GCC, GCG, and GCU all encode the amino acid alanine. Therefore, at each position where a codon specifies alanine, the codon can be changed to any of the corresponding codons described without changing the encoded polypeptide. Such nucleic acid variations are 'silent variations', which are one of conservatively modified variations. Each nucleic acid sequence encoding a polypeptide herein also describes each possible silent variation of the nucleic acid. The technician will recognize that each codon in the nucleic acid (except AUG, which is usually the only codon for methionine, and TGG, which is usually the only codon for tryptophan) can be modified to produce functionally identical molecules. Therefore, each silent variation of the nucleic acid encoding the polypeptide is implied in each of the described sequences.

[0067] For polypeptide sequences, 'conservatively modified variants' include single substitutions, deletions or additions to a polypeptide sequence, whereby an amino acid is substituted with a chemically similar amino acid. Conservative substitution tables providing functionally similar amino acids are known in the art. Such conservatively modified variants are in addition to, and do not exclude, polymorphic variants, interspecies homologs and alleles. The following eight groups contain amino acids that are conservative substitutions for each other: 1) Alanine (A), Glycine (G); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); 7) Serine (S), Threonine (T); and 8) Cysteine ​​(C), Methionine (M) (see, e.g., Creighton, Proteins (1984)). In some embodiments, the phrase 'conservative sequence modification' is used to refer to amino acid modifications that do not significantly affect or alter the binding characteristics of the binding domain of the engineered protein of the present disclosure.

[0068] 'Protein variants' or 'variants of proteins' as referred to herein relate to proteins comprising variations in which one or more, e.g., 2, 3, 4, 5, 6, 7, 8, 9, 10 amino acids have been modified. 'Functional variants' of proteins as referred to herein relate to protein variants, which comprise modifications that result in a change in the amino acid sequence but do not alter the overall properties of the protein or its function. 'Truncate variants' of a protein or a domain of a protein as referred to herein relate to shortened forms of a protein or a domain of a protein, but the shortened form of the protein retains the function of the parent protein. In order to determine whether a functional variant or a truncated variant has no change in overall properties or function, the effects of these variant proteins can be tested for full-length or unmodified parent proteins in a number of os assays as described in the present disclosure. For example, promoting endothelial cell efferocytosis in a human endothelial cell-Jurkat cell efferocytosis assay, restoring impaired macrophage efferocytosis in a human macrophage-neutrophil efferocytosis assay, reducing the number of plasma microparticles by clearing them in a human endothelial cell-microparticle efferocytosis assay, and / or providing protection against multi-organ damage in an acute renal ischemia model.

[0069] In the context of two or more nucleic acid or polypeptide sequences, the term 'percent identity' or 'percent sequence identity' refers to two or more identical sequences or subsequences. Two sequences are 'substantially identical' and show 'sequence identity' if they have a specified percentage of identical amino acid residues or nucleotides (i.e., at least 60% identity, optionally at least 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identity over a specified region or, if not specified, over the entire sequence) when compared and aligned over a comparison window or specified region to achieve maximum correspondence as measured, for example, using one of the following sequence comparison algorithms or by manual alignment and visual inspection. Optionally, the identity exists over a region that is at least about 50 nucleotides (or 10 amino acids) in length, or over a region that is 100 to 500 or 1000, or 2000 or 3000 or more nucleotides in length, or alternatively over a region that is 30 to 200 or 300 or 500 or 700 or 800 or 900 or 1000 or more amino acids in length.

[0070] For sequence comparison, typically a sequence serves as a reference sequence, and a test sequence is compared with the reference sequence. When using a sequence comparison algorithm, the test sequence and the reference sequence are input into a computer, and subsequence coordinates are specified if necessary, and sequence algorithm program parameters are specified. Default program parameters can be used, or alternative parameters can be specified. Then, the sequence comparison algorithm will calculate the sequence identity percentage of the test sequence relative to the reference sequence based on the program parameters.

[0071] As used herein, the term "comparison window" includes reference to a segment having any one of the number of contiguous nucleic acid or amino acid positions selected from the group comprising 20 to 600, typically about 50 to about 200, more typically about 100 to about 150, wherein a sequence can be compared to a reference sequence having the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are known in the art. For example, by the local homology algorithm of Smith and Waterman, (1970) Adv. Appl. Math. 2:482c, by the homology alignment algorithm of Needleman & Wunsch (1970) J. Mol. Biol. 48:443, by the study of similarity methods of Pearson & Lipman (1988) PNAS USA 85:2444, by computer implementations of these algorithms (GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package at Genetics Computer Group, 575 Science Dr., Madison, WI), or by manual alignment and visual inspection (see, e.g., Brent et al., (2003) Current Protocols in Molecular Biology). Biology [Molecular Biology Experiment Guide]), which can be used to optimally align sequences for comparison.

[0072] Two examples of algorithms suitable for determining percent sequence identity and sequence similarity are the BLAST and BLAST 2.0 algorithms, which are described in Altschul et al. (1977) Nuc. Acids Res. 25:3389-3402; and Altschul et al. (1990) J. Mol. Biol. 215:403-410, respectively. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information.

[0073] The BLAST algorithm also performs a statistical analysis of the similarity between two sequences (see, e.g., Karlin and Altschul (1993) PNAS. USA, 90:5873-5787). One measure of similarity provided by the BLAST algorithm is the smallest sum probability (P(N)), which provides an indication of the probability that a match between two nucleotide or amino acid sequences would occur by chance. For example, a nucleic acid is considered similar to a reference sequence if the smallest sum probability in a comparison of the test nucleic acid to the reference nucleic acid is less than about 0.2, more preferably less than about 0.01, and most preferably less than about 0.001.

[0074] The percent identity between two amino acid sequences can also be determined using the algorithm of E. Meyers and W. Miller (Comput. Appl. Biosci., 4: 11-17 (1988)), which has been incorporated into the ALIGN program (version 2.0), using a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4. In addition, the percent identity between two amino acid sequences can be determined using the Needleman & Wunsch (supra) algorithm, which has been incorporated into the GAP program in the GCG software package (available at www.gcg.com), using a Blossum 62 matrix or a PAM250 matrix and a gap weight of 16, 14, 12, 10, 8, 6 or 4 and a length weight of 1, 2, 3, 4, 5 or 6.

[0075] A polypeptide is typically substantially identical to a second polypeptide, for example where the two peptides differ only by conservative substitutions.Another indication that two nucleic acid sequences are substantially identical is that the two molecules, or their complements, hybridize to each other under stringent conditions.

[0076] The term 'nucleic acid' is used interchangeably with the term 'polynucleotide' herein and refers to deoxyribonucleotides or ribonucleotides and polymers thereof in single-stranded or double-stranded form. The term encompasses nucleic acids containing known nucleotide analogs or modified backbone residues or linkages that are synthetic, naturally occurring, and non-naturally occurring, have similar binding properties to reference nucleic acids, and are metabolized in a manner similar to reference nucleotides. Examples of such analogs include, but are not limited to, phosphorothioates, phosphoramidates, methylphosphonates, chiral-methylphosphonates, 2-O-methyl ribonucleotides, peptide-nucleic acids (PNAs).

[0077] Unless otherwise indicated, a particular nucleic acid sequence also implicitly encompasses conservatively modified variants thereof (e.g., degenerate codon substitutions) and complementary sequences as well as explicitly indicated sequences. Specifically, degenerate codon substitutions can be achieved by generating sequences in which the third position of one or more selected (or all) codons is substituted with mixed bases and / or deoxyinosine residues (Batzer et al., (1991) Nucleic Acid Res., 19:5081; Ohtsuka et al., (1985) J Biol Chem., 260:2605-2608; and Rossolini et al., (1994) Mol Cell Probes, 8:91-98). As used herein, the term 'optimized nucleotide sequence' means that the nucleotide sequence has been altered to encode an amino acid sequence using codons that are preferred in a production cell (e.g., Chinese hamster ovary cells (CHO)). The optimized nucleotide sequence is engineered to retain exactly the amino acid sequence originally encoded by the starting nucleotide sequence, also referred to as the 'parent' sequence. In specific embodiments, the optimized sequences herein have been engineered to have codons that are preferred in CHO mammalian cells.

[0078] Therapeutic fusion proteins

[0079] Integrin binding domain

[0080] Integrins are transmembrane receptors that promote cell-extracellular matrix (ECM) adhesion. Upon ligand binding, integrins activate signal transduction pathways that mediate cell signaling, such as regulation of the cell cycle, organization of the intracellular cytoskeleton, and movement of new receptors to the cell membrane (Giancotti & Ruoslahti (1999) Science [Science], 285(5430): 1028-32). The presence of integrins allows for rapid and flexible responses to events on the cell surface. There are many types of integrins, and a cell may have many different types on its surface. Integrins have two subunits: α (alpha) and β (beta), each of which penetrates the plasma membrane and has several cytoplasmic domains (Nermut MV et al. (1988). EMBO J. [Journal of the European Molecular Biology Association], 7(13): 4093-9). The acidic amino acids characteristic of the integrin interaction sites of many ECM proteins are, for example, as part of the amino acid sequence arginine-glycine-aspartic acid (single letter amino acid code 'RGD'). The RGD motif has been found in numerous matrix proteins (e.g., fibronectin, fibrinogen, vitronectin, and osteopontin) and aids in cell adhesion. The RGD motif is found in a conserved protein domain called the EGF-like domain in many proteins, the name of which derives from the epidermal growth factor from which it was originally described. The EGF-like domain is one of the most common domains in extracellular proteins (Hidai C (2018) Open Available J Trans Med Res. [Journal of Translational Research], 2(2): 67-71) and some examples of EGF-like domains containing the RGD binding motif are listed in Table 1 below.

[0081] Table 1: Examples of proteins comprising an EGF-like domain protein containing an RGD integrin binding motif

[0082]

[0083] As used herein, the term "integrin binding domain" refers to a stretch of amino acids or a protein domain that has a function of binding to an integrin. In one embodiment of the present disclosure, as used herein, 'integrin binding domain' refers to a stretch of amino acids or a protein domain that has a function of binding to an integrin and contains an RGD motif. In one embodiment of the present disclosure, the integrin binding domain is an EGF-like domain from human MFG-E8 having an amino acid sequence as shown in SEQ ID NO: 2. In an alternative embodiment of the present disclosure, the integrin binding domain is an EGF-like domain from human EDIL3 (any one of the following sequences: SEQ ID NO: 11, SEQ ID NO: 77, SEQ ID NO: 96, SEQ ID NO: 97, SEQ ID NO: 98, SEQ ID NO: 99, SEQ ID NO: 100 or SEQ ID NO: 101); for example, the EGF-like domain can be found in the segment of amino acids 1-132 of SEQID NO: 11.

[0084] As used herein, the term 'binding to an integrin' refers to integrin binding activity. Integrin binding activity can be determined by methods well known in the art. For example, in Section 3.2 of the Examples, an integrin adhesion assay is described, in which the adhesion of lymphoma cells expressing fluorescently labeled αvβ3 integrin to the therapeutic fusion proteins of the present disclosure is determined. If the integrin binding domain has at least 10% of the integrin binding activity observed for human MFG-E8 protein (SEQ ID NO: 1), such as at least 25%, at least 50%, at least 75%, more preferably at least 80%, such as at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, then the integrin binding domain is considered to have integrin binding activity (this is when the respective activities are determined by the same method, preferably when tested by the assay described in the Examples of Section 3.2).

[0085] Phosphatidylserine binding domain

[0086] As used herein, 'phosphatidylserine' (PS) refers to phospholipids that are components of cell membranes. PS is primarily confined to the inner leaflet of the cell membrane, while phosphatidylcholine and sphingomyelin are primarily located in the outer leaflet. The asymmetric distribution of phospholipids is maintained by the action of flippase (P4-ATPase, such as ATP11A and 11C) in the plasma membrane, which actively translocates PS from the outer leaflet to the inner leaflet. Cell surface exposure of PS is observed not only in apoptotic cells, but also in activated lymphocytes, activated platelets, aging erythrocytes, and some cancer cells and their respective microparticles (Sakuragi et al., (2019) PNAS USA [Proceedings of the National Academy of Sciences of the United States], 116 (8): 2907-12). PS exposure can be a biomarker of prothrombotic, inflammatory, or ischemic disease states (Pasalic et al. (2018) J Thromb Haemost. 16(6):1198-2010; Ma et al. (2017) supra; Zhao et al. (2016) supra). PS has functions in multiple cell signaling pathways and serves as an essential phospholipid in the coagulation process, where it can act as an enhancer of the formation of the tenase (factors IXa, VIIIa, and X) and prothrombinase (factors Xa, Va, and prothrombin) complexes (Spronk et al. (2014) Thromb Res. 133 (Suppl. 1): S54-6). The most likely understood function of externalized PS remains as an "eat me" marker for phagocytic cells (e.g., macrophages) to engulf apoptotic cells, cell debris, or activated cells exposed to PS. As used herein, the term 'phosphatidylserine binding domain' or 'PS binding domain' refers to a stretch of amino acids or a protein domain that has the function of binding PS. Examples of endogenous proteins with PS binding domains can be found in Table 2 below.

[0087] Table 2: Examples of receptors / proteins with phosphatidylserine binding domains

[0088]

[0089]

[0090] In one embodiment of the present disclosure, the PS domain is derived from human MFG-E8 having an amino acid sequence as shown in SEQ ID NO: 3. In an alternative embodiment of the present disclosure, the integrin binding domain is a PS binding domain from human EDIL3 (SEQ ID NO: 11), wherein the PS binding domain comprises amino acids 135-453 of SEQ ID NO: 11.

[0091] PS binding activity can be determined by methods well known in the art. For example, a PS binding assay is described in Example Section 3.1, in which the binding of the fusion protein of the present disclosure to PS coated on a microtiter plate is evaluated by competition with the binding of biotinylated murine MFG-E8. According to the present disclosure, if the PS binding domain has at least 10% of the PS binding activity observed for human MFG-E8 protein (shown in SEQ ID NO: 1), such as at least 25%, at least 50%, at least 75%, at least 80%, preferably at least 90%, at least 95%, at least 96%, at least 97%, at least 98%, then the PS binding domain is considered to have PS binding activity (this is when the respective activities are determined by the same method, preferably when tested by the assay described in the Examples of Section 3.1).

[0092] Bridging Protein

[0093] There are many endogenous proteins that contain both an integrin binding domain and a PS binding domain. Examples of such "bridging proteins" are shown in Table 3 below.

[0094] Table 3: Bridging proteins containing integrin and phosphatidylserine binding domains

[0095]

[0096]

[0097] To be of therapeutic value, it would be useful if the bridging protein contained an integrin binding domain that recognized an integrin on phagocytes that is typically insensitive to proteolytic cleavage or shedding, as observed in TAM family members or other PS binding receptors. Proteins with both a PS binding domain and an integrin binding domain, such as MFG-E8 or its paralog EDIL3 / DEL1, have been shown to induce efferocytosis in vitro and therefore have therapeutic value as inducers of efferocytosis in AOI. In contrast, for example, the GAS6 protein may not be particularly effective in promoting efferocytosis in AOI because, as described above, its phagocytic receptor on phagocytes (MerTK) is proteolytically cleaved during inflammation and infection.

[0098] As shown in Table 3 above, an example of a bridging protein is MFG-E8, which is one of the major proteins found in the milk fat globule membrane (MFGM). MFG-E8 is expressed by several different types of cells (e.g., mammary epithelial cells, vascular cells, epididymal epithelial cells, aortic smooth muscle cells, activated macrophages, stimulated endometrium, and immature dendritic cells) and tissues (e.g., heart, lung, mammary gland, spleen, intestine, liver, kidney, brain, blood, and endothelium). The MFG-E8 protein also has several different names, such as lactadherin, BP47, component 15 / 16, MFGM, MGP57 / 53, PAS-6 / PAS-7 glycoprotein, cell wall protein SED1, sperm surface protein SP47, mammary epithelial antigen BA46, and O-acetyl GD3 ganglioside synthase (AGS). The MFG-E8 gene is located on chromosome 1 of rats, chromosome 7 of mice, and chromosome 15 of humans. Alternative splicing of the pre-mRNA of MFG-E8 results in three isoforms of the human protein and two forms of mRNA, long and short variants, expressed in the mouse mammary gland. The human MFG-E8 gene (UniProtKB-Q08431) encodes a 387-residue protein that can be processed into multiple protein products. The amino acid sequence of human MFG-E8 (including the signal peptide (residues 1-23; underlined), the EGF-like domain (residues 24-67; italic), the C1 domain (residues 70-225; bold), and the C2 domain (residues 230-387; bold and underlined)) is provided below:

[0099]

[0100] MFG-E8 lacks the transmembrane function possessed by MFGM and can therefore act as a peripheral membrane protein. Human MFG-E8 consists of an N-terminal EGF-like domain (SEQ ID NO: 2) that binds to αvβ3 and αvβ5 integrins expressed on phagocytes and a PS binding domain (SEQ ID NO: 3) containing two F5 / 8-dictyol lectin subdomains (C1 and C2) that bind to anionic phospholipids with high affinity. Integrin binding is the result of the RGD motif located in residues 46-48 of human MFG-E8 (SEQ ID NO: 1). Apoptotic cells, cell debris, hyperactivated cells, and most microparticles (MPs) expose PS and are targets of MFG-E8, which acts as a bridging molecule, opsonizing these cells and microparticles and connecting them to αvβ3 and αvβ5 integrins on phagocytes. This bridging action triggers an efficient phagocytic program, leading to the internalization of cells, debris, and microparticles. The proteins found in MFGM are highly conserved across species. The protein structure of MFG-E8 varies from species to species; all currently known species contain two C domains, but the number of EGF-like domains varies. For example, the human MFG-E8 protein contains one EGF-like domain, while bovine MFG-E8 and murine MFG-E8 (SEQ ID NO:68) have two EGF-like domains, and chicken, frog, and zebrafish have three EGF-like-domains. Domains of MFG-E8 have been previously proposed as components of therapeutic agents, in particular the PS-binding domain of MFG-E8 (Kooijmans et al., (2018) Nanoscale, 10(5):2413-2426) and fragments have been described as functional in models of fibrosis (U.S. Patent Application US2018 / 0334486).

[0101] Non-inflammatory uptake of dying cells, debris and particulates by both professional and non-professional phagocytes plays a crucial role in homeostasis following tissue injury (Greenlee-Wacker (2016) supra). The importance of proper clearance becomes even more apparent in genetic models where MFG-E8 knockout mice show, for example, increased numbers of (uncleared) dying cells in tissues and increased inflammatory responses in disease models such as neonatal sepsis, autoimmunity, poor angiogenesis and impaired wound healing (Hanayama et al. (2004) Science, 204(5474):1147-50; Das et al. (2016) J Immunol., 196(12):5089-5100; Hansen et al. (2017) J Pediatr Surg., 52(9):1520-7).

[0102] In addition, MFG-E8 has been shown to generate a tolerogenic environment by inhibiting T cell activation and proliferation, suppressing Th1, Th2 and Th17 subsets while increasing regulatory T cell subsets (Treg). Interestingly, Treg contributes to the resolution of inflammation by inducing macrophage efferocytosis (Proto et al., (2018) Immunity, 49(4): 666-77). MFG-E8 has been described to promote allogeneic transplantation of embryonic stem cell-derived tissues across the MHC barrier (Tan et al., (2015) Stem Cell Reports, 5(5): 741-752). MFG-E8 also has a variety of nutritional uses that help promote tissue development and protection against infectious agents. Glycoproteins such as MFG-E8 are potential health-enhancing nutritional products for food and pharmaceutical applications. MFG-E8 can also be used in combination with other nutrients such as probiotics, whey protein micelles, α-hydroxyisocaproic acid, citrulline and branched-chain fatty acids.

[0103] Solubilization domain

[0104] As described herein, the therapeutic fusion proteins disclosed herein comprise an integrin binding domain and a PS binding domain. In addition, the fusion protein further comprises additional domains that confer many desired properties to the fusion protein. The additional domains (referred to as 'solubilization domains' for the purposes of this application) confer improved biological properties, such as increased solubility, reduced aggregation, and increased biological activity. As a result, the fusion protein exhibits a desired pharmacokinetic profile. In addition, the presence of the solubilization domain improves the stability of the therapeutic fusion protein and improves the expression of the fusion protein in a cell expression system compared to the wild-type protein, as indicated by an increase in yield after purification.

[0105] The presence of a solubilizing domain can also confer a prolonged half-life on therapeutic fusion proteins. For example, many protein drugs are connected to polyethylene glycol (PEG), reCODE PEG, antibody scaffolds, polysialic acid (PSA), hydroxyethyl starch (HES) and serum proteins (such as albumin, IgG and FcRn) to extend their plasma half-life and achieve enhanced therapeutic effects (Kim et al., (2010) J Pharmacol Exp Ther. [Journal of Pharmacology and Experimental Therapeutics], 334: 682-92; Weimer et al., (2008) Thromb Haemost. [Thrombosis and hemostasis] 99: 659-67; Dumont et al., (2006) BioDrugs [Biological Drugs], 20: 151-60; Schellenberger et al., (2009) Nat Biotechnol. [Natural Biotechnology], 27: 1186-90).

[0106] In some embodiments, the solubilizing domain is an albumin protein, such as human serum albumin (HSA; SEQ ID NO: 4) or a variant thereof. For example, HSA comprises an amino acid substitution C34S (SEQ ID NO: 5) with a lower tendency to aggregate, or a domain of HSA, such as HSA D3; (SEQ ID NO: 6). Due to a number of factors, including its relatively large size (which can reduce renal filtration) and its neonatal Fc receptor (FcRn) binding characteristics, HSA has a very long serum half-life, thereby avoiding intracellular degradation. It has also been proposed to use an N-terminal fragment of HSA to fuse with a polypeptide (e.g., patent application EP 399666). Therefore, genetically or chemically fusing or conjugating the molecule to albumin can stabilize or extend the shelf life, and / or retain the activity of the molecule in solution, in vitro and / or in vivo for an extended period of time. Additional methods for HSA fusion can be found, for example, in international patent applications WO 2001 / 077137 and WO2003 / 060071.

[0107] In some embodiments, the solubilizing domain comprises an antibody Fc domain, such as human Fc-immunoglobulin G1 (Fc-IgG1; SEQ ID NO: 7). The Fc domain can also be modified, for example, by using a knob-hole (KiH)-based modification (by introducing complementary amino acid substitutions in the CH3 domain of Fc) to improve heterodimerization of Fc. For example, substitution T366W produces a 'knob' on one CH3 domain, and substitutions T366S, L368A, and Y407V produce a 'hole' on another CH3 domain (Merchant et al. (1998) Nat. Biotechnol. [Nature Biotechnology], 16 (7): 677-81; EU numbering IgG1). Additional modifications that may be included in the Fc domain, alone or in combination with modifications, to improve heterodimerization may include, for example, amino acid substitutions to cysteine ​​to create additional cysteine ​​bonds, such as S354C and / or Y349C, and amino acid substitutions that reduce or eliminate binding to Fcγ receptors and complement protein C1q to 'silence' immune effector function. The so-called 'LALA' double mutation (L234A and L235A together; EU numbering) results in reduced effector function (Lund et al., (1992) Mol Immunol. [Molecular Immunology], 29: 53-9). In addition, the 'DAPA' double mutation (D265A and P329A; EU numbering) results in reduced effector function. In one embodiment of the present disclosure, the Fc domain may include amino acid substitutions D265A, P329A and / or KiH amino acid substitutions T366W (knob) or T366S, L368A and Y407V (hole) for Fc silencing. In one embodiment, the Fc domain is derived from human IgG1 and comprises amino acid substitutions D265A, P329A (SEQ ID NO: 8). In another embodiment, the Fc domain is derived from human IgG1 and comprises amino acid substitutions D265A, P329A, S354C and amino acid substitutions T366W (Fc-IgG1-knob; SEQ ID NO: 9). In another embodiment, the Fc domain is derived from human IgG1 and comprises amino acid substitutions D265A, P329A, Y349C and amino acid substitutions T366S, L368A and Y407V (Fc-IgG1-hole; SEQ ID NO: 10).

[0108] In some embodiments, the solubilizing domain comprises an antibody Fc domain derived from human IgA, IgD, IgE, or IgM.

[0109] In some embodiments, the solubilizing domain comprises SUMO (small ubiquitin-like modifier), ubiquitin, GST (glutathione S-transferase), or variants thereof.

[0110] Linking and Orienting Domains of Therapeutic Fusion Proteins

[0111] The integrin binding domain, PS binding domain and solubilizing domain of the fusion protein of the present disclosure are connected. As used herein, the term 'connected' or 'connected' means that one domain of the fusion protein is directly or indirectly attached to another domain of the fusion protein. Direct attachment is a form of connection and is referred to as 'fused' or 'fusion' in this article. Take a molecule with an ABC form as an example: domain A is directly connected to domain B, and directly connected to domain C. In this way, domain A can also be described as fused to domain B, and domain B is fused to domain C. As another example, domain A is directly connected to domain B, and indirectly connected to domain C. In this way, domain A can also be described as fused to domain B, and domain B is indirectly connected to domain C through an internal linker.

[0112] In some embodiments, the connection is a direct connection, and thus the domains are fused to each other. In some embodiments, the integrin binding domain is fused to the PS binding domain, and the PS binding domain is fused to the solubilization domain. Specifically, the PS binding domain (e.g., C1-C2 lectin subdomain) is fused to the C-terminus of the integrin binding domain (e.g., EGF-like domain) and to the N-terminus of the solubilization domain (e.g., HSA). In some embodiments, the solubilization domain is fused to the integrin binding domain, and the integrin binding domain is fused to the PS binding domain. Specifically, the integrin binding domain (e.g., EGF-like domain) is fused to the C-terminus of the solubilization domain (e.g., HSA) and to the N-terminus of the PS binding domain (e.g., C1-C2 lectin subdomain). In some embodiments, the integrin binding domain is fused to a PS binding domain comprising C1-C2 lectin subdomains, and the solubilization domain is inserted between the C1-C2 lectin subdomains. Specifically, the C-terminus of the integrin binding domain (e.g., EGF-like domain) is fused to the N-terminus of the C1 lectin subdomain, and the C-terminus of the C1 lectin subdomain is fused to the N-terminus of the solubilization domain (e.g., HSA), and the C-terminus of the solubilization domain is fused to the N-terminus of the C2 lectin subdomain. In another embodiment, the integrin binding domain is fused to the solubilization domain, and the solubilization domain is fused to the PS binding domain. Specifically, the solubilization domain (e.g., HSA) is fused to the C-terminus of the integrin binding domain (e.g., EGF-like domain) and to the N-terminus of the PS binding domain (e.g., C1-C2 lectin subdomain). In one embodiment, HSA is fused to the C-terminus of the EGF-like domain and to the N-terminus of the C1 Dictyostelium domain.

[0113] In some embodiments, the solubilization domain (eg, HSA) is fused between the integrin binding domain and the PS binding domain. In some embodiments, the integrin binding domain is located at the N-terminus of the fusion protein, and the PS binding domain is located at the C-terminus of the fusion protein.

[0114] In some embodiments, the fusion protein comprises a first region comprising an integrin binding domain (e.g., an EGF-like domain), a second region comprising a solubilization domain (e.g., HSA or Fc), and a third region comprising a PS binding domain (e.g., C1 and / or C2 Dictyostelium lectin domain). In some embodiments, the integrin binding domain is located at the N-terminus of the fusion protein, and the PS binding domain is located at the C-terminus of the fusion protein.

[0115] In some embodiments, the solubilizing domain (eg, HSA or Fc) is HSA.

[0116] In some embodiments, the solubilizing domain is HSA or a functional variant thereof.

[0117] In some embodiments, the solubilizing domain is antibody Fc-immunoglobulin G1 (Fc-IgG1; SEQ ID NO: 7).

[0118] In a preferred embodiment, the amino acid sequence shown in SEQ ID NO: 5 is fused to the C-terminus of HSA and the EGF-like domain of MFG-E8, and fused to the N-terminus of the PS binding domain of MFG-E8. In one embodiment, the fusion protein comprises the amino acid sequence shown in SEQ ID NO: 46 (FP068). In one embodiment, the fusion protein comprises the amino acid sequence shown in SEQ ID NO: 48 (FP776).

[0119] In an alternative embodiment, the amino acid sequence of SEQ ID NO: 5 is fused to the C-terminus of HSA and the EGF-like domain of EDIL3, and fused to the N-terminus of the PS binding domain of EDIL3. In one embodiment, the fusion protein comprises the amino acid sequence of SEQ ID NO: 70 (FP1068). In one embodiment, the fusion protein comprises the amino acid sequence of SEQ ID NO: 69 (FP1776).

[0120] In some embodiments, the connection is through a polypeptide linker and, for example, the polypeptide linker that connects the solubilization domain to the PS binding domain in the fusion protein of the present disclosure is referred to as an 'external linker'. These external linkers typically contain glycine (G) and / or serine (S), and may also contain glycine and leucine (GL) or glycine and valine (GL). In some embodiments, the linker contains multiple G and S residues, such as G 2 S and multiples thereof, such as (G 2 S) 4 , (GS) shown in SEQ ID NO:63 4 , G shown in SEQ ID NO:64 4 S or (G shown in SEQ ID NO:65 4 S) 2 .

[0121] In some embodiments, the external linker is fused between the C-terminus of the integrin binding domain and the N-terminus of the solubilization domain. Specifically, the external linker is fused to the C-terminus of the EGF-like domain and the N-terminus of HSA. In some embodiments, the external linker is fused between the C-terminus of the solubilization domain and the N-terminus of the PS binding domain. Specifically, the external linker is fused to the C-terminus of HSA and the N-terminus of the PS binding domain. In some embodiments, the external linker is fused between the C-terminus of the integrin binding domain and the N-terminus of the solubilization domain, and an additional external linker is fused between the C-terminus of the solubilization domain and the N-terminus of the PS binding domain. Specifically, the external linker is fused to the C-terminus of the EGF-like domain and the N-terminus of HSA, and an additional external linker is fused to the C-terminus of HSA and the N-terminus of the PS binding domain.

[0122] In some embodiments, an external linker comprising GS is fused to the C-terminus of the integrin binding domain and to the N-terminus of the solubilization domain. In some embodiments, an external linker comprising GL is fused to the C-terminus of the solubilization domain and to the N-terminus of the PS binding domain. 2 S) 4 (SEQ ID NO: 62) is fused to the C-terminus of the solubilization domain and to the N-terminus of the PS binding domain. 4 An external linker of S (SEQ ID NO: 64) is fused to the C-terminus of the solubilization domain and to the N-terminus of the PS binding domain. In some embodiments, the invention comprises (G 4 S) 2 An external linker of (SEQ ID NO: 65) is fused to the C-terminus of the solubilization domain and to the N-terminus of the PS binding domain.

[0123] In one embodiment, the external linker comprising GS is fused to the C-terminus of the EGF-like domain and to the N-terminus of HSA. The fusion protein comprising the structure disclosed herein has the amino acid sequence shown in SEQ ID NO: 42 (FP330).

[0124] In one embodiment, an external linker comprising GS is fused to the C-terminus of the EGF-like domain and to the N-terminus of HSA, and comprises (GS) 4 An additional external linker of (SEQ ID NO: 63) was fused to the C-terminus of HSA and to the N-terminus of the PS binding domain.

[0125] In one embodiment, an external linker comprising GS is fused to the C-terminus of the EGF-like domain and to the N-terminus of HSA, and comprises (G 2 S) 4 The additional external linker of (SEQ ID NO: 62) is fused to the C-terminus of HSA and to the N-terminus of the PS binding domain. The fusion protein comprising the structure disclosed herein has the amino acid sequence shown in SEQ ID NO: 42 (FP330).

[0126] In one embodiment, an external linker comprising GS is fused to the C-terminus of the EGF-like domain and to the N-terminus of HSA. The C-terminus of HSA is fused directly to the N-terminus of the PS binding domain.

[0127] In one embodiment, an external linker comprising GS is fused to the C-terminus of the EGF-like domain and to the N-terminus of HSA, and comprises G 4 The additional external linker of S (SEQ ID NO: 64) is fused to the C-terminus of HSA and to the N-terminus of the PS binding domain. The fusion protein comprising the structure disclosed herein has the amino acid sequence shown in SEQ ID NO: 54 (FP811).

[0128] In one embodiment, an external linker comprising GS is fused to the C-terminus of the EGF-like domain and to the N-terminus of HSA, and comprises (G 4 S) 2 The additional external linker of (SEQ ID NO: 65) is fused to the C-terminus of HSA and to the N-terminus of the PS binding domain. The fusion protein comprising the structure disclosed herein has the amino acid sequence shown in SEQ ID NO: 56 (FP010).

[0129] In some embodiments, the His tag is fused to an external linker comprising GS (GS-6xHis; SEQ ID NO: 66), wherein the GS is fused to the C-terminus of the PS binding domain. In one embodiment, the fusion protein comprising a His tag of the present disclosure has an amino acid sequence as shown in SEQ ID NO: 44 (FP278) or SEQ ID NO: 60 (FP114 or FP260).

[0130] Functional characterization of therapeutic fusion proteins

[0131] The present disclosure provides fusion proteins derived from human MFG-E8, and the fusion proteins are effective in promoting efferocytosis and are therefore active in eliminating key drivers of systemic inflammation and microvascular pathology. As described in the Examples, fusion proteins having the general structure of EGF-HSA-C1-C2 have been shown to be effective in many efferocytosis assays. For example, the fusion protein has effectively restored efferocytosis of macrophages impaired by lipopolysaccharide (LPS) or Staphylococcus aureus, and enhanced efferocytosis of endothelial cells to microparticles and dying cells. In a mouse model of acute renal injury, the fusion protein has also been effective in protecting renal function and preventing weight loss.

[0132] Exemplary protein sequences

[0133] The amino acid sequences in Table 4 include examples of therapeutic fusion proteins of the disclosure and portions thereof.

[0134] Throughout this application, if there is a discrepancy between the specification text (eg, Table 4) and the sequence listing, the specification text shall prevail.

[0135] Table 4. Exemplary protein sequences

[0136]

[0137]

[0138]

[0139]

[0140]

[0141]

[0142]

[0143]

[0144]

[0145]

[0146]

[0147]

[0148]

[0149]

[0150]

[0151]

[0152]

[0153]

[0154]

[0155]

[0156]

[0157]

[0158]

[0159]

[0160]

[0161]

[0162]

[0163]

[0164]

[0165]

[0166]

[0167]

[0168]

[0169]

[0170]

[0171]

[0172]

[0173]

[0174]

[0175]

[0176]

[0177]

[0178]

[0179]

[0180]

[0181]

[0182]

[0183]

[0184] The present application also includes variants of each of SEQ ID NOs:69, 70 and 72, wherein the EGF-like domain of the EDIL3 sequence included in the variant corresponds to any one of the following sequences: SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:100 or SEQ ID NO:101.

[0185] The present application also includes therapeutic fusion proteins comprising the integrin binding domain of MFGE8 or EDIL3 and a truncated PS binding domain, such as a truncated variant of the IgSF V domain of TIM4 or a truncated variant of the GLA domain of the bridging protein GAS6 variant.

[0186] Modification of proteins disclosed herein

[0187] The application includes variants and / or fragments thereof with various modifications in the domain of protein as described herein, and fusions and conjugates of disclosed molecules.For example, the domain of therapeutic fusion protein can have the conservative modification of amino acid residues, and wherein compared with the fusion protein comprising the parental domain, the modified protein retains or has enhanced characteristics.Alternately, the domain of therapeutic fusion protein can have the disappearance of one or more amino acid residues, wherein compared with the protein comprising the parental domain, the modified fusion protein retains or has enhanced characteristics.Alternately, the therapeutic fusion protein can have the insertion of one or more amino acid residues, wherein compared with the unmodified protein, the modified protein retains or has enhanced characteristics.In one embodiment, such amino acid insertion includes glycine or serine residues with multiple combinations, as a joint between the domains of the parental protein.

[0188] Site-directed mutagenesis or PCR-mediated mutagenesis can be performed to introduce one or more mutations, and the effect on integrin and / or PS binding or other functional properties of interest can be evaluated in in vitro or in vivo assays. Conservative modifications (as described above) can be introduced and / or mutations can be amino acid substitutions, additions or deletions. Furthermore, typically no more than one, two, three, four or five residues within a binding domain are altered.

[0189] Amino acid sequence variants with therapeutic fusion proteins (whose characteristics are substantially similar to unmodified variants) can be prepared by introducing appropriate nucleotide changes into the encoding DNA or by synthesizing desired variants. Such variants include, for example, deletions, insertions or substitutions of residues in the amino acid sequence of the molecule of the present invention. In certain embodiments, the variant may include additional linker sequences, removal of reduced linker sequences or linker sequences, and / or amino acid mutations or substitutions and deletions of one or more amino acids. Any combination of deletions, insertions and substitutions may be performed to obtain the final construct, provided that the final construct has the desired characteristics. Amino acid changes may also change the post-translational processes of the molecule, such as changing the number or position of possible glycosylation sites.

[0190] Methods for producing recombinant molecules

[0191] Nucleic Acids and Expression Systems

[0192] In one embodiment, the present application provides a method for recombinantly producing one or more polypeptide chains of a therapeutic fusion protein, the method comprising: 1) generating one or more DNA constructs, the DNA constructs comprising nucleic acid molecules encoding polypeptide chains of multi-specific binding molecules; 2) introducing the one or more DNA constructs into one or more expression vectors; 3) co-transfecting the one or more expression vectors into one or more host cells; and 4) expressing and assembling the molecules in host cells or solutions.

[0193] In this regard, the disclosure provides isolated nucleic acids encoding therapeutic fusion proteins described herein, such as one or more polynucleotides. Nucleic acid molecules include DNA and RNA in single-stranded and double-stranded forms, and corresponding complementary sequences. Nucleic acid molecules of the present invention include full-length genes or cDNA molecules and combinations of fragments thereof. Nucleic acids of the present invention are derived from human sources, but the present invention includes nucleic acids derived from non-human species.

[0194] 'Isolated nucleic acid' is a nucleic acid separated from adjacent genetic sequences present in the genome of an organism from which the nucleic acid is isolated in the case of nucleic acids isolated from naturally occurring sources. In the case of nucleic acids (such as PCR products, cDNA molecules, or oligonucleotides) synthesized enzymatically from a template or chemically, it is understood that the nucleic acid produced by such a process is an isolated nucleic acid. An isolated nucleic acid molecule refers to a nucleic acid molecule in the form of a single fragment or as a component of a larger nucleic acid construct. In a preferred embodiment, the nucleic acid is substantially free of contaminating endogenous materials. The nucleic acid molecule is preferably derived from a DNA or RNA separated at least once in a substantially pure form and in an amount or concentration that enables identification, manipulation, and recovery of its component nucleotide sequences by standard biochemical methods (such as those outlined in Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd edition, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY (1989)). Such sequences are preferably provided and / or constructed in the form of an open reading frame that is not interrupted by internal non-translated sequences or introns that are typically present in eukaryotic genes. Sequences of non-translated DNA may be present 5' or 3' to the open reading frame where such sequences do not interfere with manipulation or expression of the coding region.

[0195] The present invention also provides expression systems and constructs in the form of plasmids, expression vectors, transcription or expression cassettes comprising at least one polynucleotide as described above. In addition, the present invention provides host cells comprising such expression systems or constructs.

[0196] In one embodiment, the present disclosure provides a method for preparing a therapeutic fusion protein, the method comprising the following steps: (a) culturing a host cell comprising a nucleic acid encoding the fusion protein, wherein the cultured host cell expresses the fusion protein; and (b) recovering the fusion protein from the host cell culture.

[0197] The present disclosure also provides expression vectors and host cells for producing the above-mentioned therapeutic fusion proteins. The term "vector" refers to any molecule or entity (e.g., nucleic acid, plasmid, phage or virus) of a nucleic acid sequence suitable for transforming or transfecting a host cell and comprising the expression of one or more heterologous coding regions operably connected thereto, which is directed and / or controlled (combined with a host cell). Various expression vectors can be used to express the polynucleotides encoding the chain or binding domain of the molecule. Both viral-based expression vectors and non-viral expression vectors can be used to produce therapeutic fusion proteins in mammalian host cells. Non-viral vectors and systems include plasmids, episomal vectors (typically with expression cassettes for expressing proteins or RNA) and human artificial chromosomes (see, e.g., Harrington et al., (1997) Nat Genet. [Natural Genetics] 15: 345). For example, non-viral vectors useful for expressing polynucleotides and polypeptides in mammalian (e.g., human) cells include pThioHis A, pThioHis B, and pThioHis C, pcDNA3.1 / His, pEBVHis A, pEBVHis B, and pEBVHis C (Invitrogen, San Diego, CA), MPSV vectors, and a variety of other vectors known in the art for expressing other proteins. Useful viral vectors include retrovirus-based, adenovirus-based, adeno-associated virus-based, herpes virus-based vectors, SV40-based, papillomavirus-based, HBP Epstein Barr virus-based vectors, vaccinia virus vectors, and Semliki Forest virus (SFV). See, Brent et al., (1995) supra; Smith, Annu. Rev. Microbiol. 49:807; and Rosenfeld et al., (1992) Cell 68:143.

[0198] The selection of expression vector depends on the expected host cell to express the vector. Typically, the expression vector contains a promoter and other regulatory sequences (e.g., enhancers) operably connected to a polynucleotide encoding a therapeutic fusion protein. In certain embodiments, an inducible promoter is used to prevent the inserted sequence from being expressed under conditions other than inducing conditions. Inducible promoters include, for example, arabinose, lacZ, metallothionein promoters or heat shock promoters. The culture of the transformed organism can be expanded under non-inducing conditions without biasing the host cell to better tolerate the coding sequence of its expression product. In addition to the promoter, other regulatory elements may also be needed or desired to efficiently express the therapeutic fusion protein. These elements typically include an ATG start codon and adjacent ribosome binding sites or other sequences. In addition, expression efficiency can be improved by including an enhancer suitable for the cell system used (see, for example, Scharf et al., (1994) Results Probl. Cell Differ. [Results and problems in cell differentiation] 20: 125; and Bittner et al., (1987) Meth. Enzymol. [Enzymology], 153: 516). For example, the SV40 enhancer or the CMV enhancer can be used to increase expression in mammalian host cells.

[0199] The expression vector can also provide a secretion signal sequence position to form a fusion protein with the polypeptide encoded by the above sequence of the insertion binding domain and / or solubilization domain. More commonly, the insertion sequence is connected to the signal sequence before being included in the vector. The carrier allows the binding domain and the solubilization domain to be expressed as a fusion protein, thereby resulting in the production of a complete engineered protein. When cultivated under appropriate conditions, the host cell can be used to express the engineered protein, and the engineered protein can be collected from the culture medium (if the host cell secretes it into the culture medium) or directly collected from the host cell that produces it (if not secretory). The selection of suitable host cells will depend on various factors, such as the desired expression level, activity (such as glycosylation or phosphorylation) expectation or necessary polypeptide modification and the cheapness of being folded into a bioactive molecule. The host cell can be a eukaryotic or prokaryotic cell.

[0200] Mammalian cell lines that can be used as expression hosts are well known in the art and include, but are not limited to, immortalized cell lines available from the American Type Culture Collection (ATCC), and any cell line used in expression systems known in the art can be used to prepare the recombinant fusion protein of the present invention. Generally speaking, host cells are transformed with a recombinant expression vector comprising a DNA encoding the desired fusion protein. Adoptable host cells are prokaryotes, yeast, or higher eukaryotic cells. Prokaryotes include gram-negative or gram-positive organisms, such as Escherichia coli (E.coli) or bacilli (bacilli). Higher eukaryotic cells include insect cells and established mammalian cell lines. Examples of suitable mammalian host cell lines include COS-7 cells, L cells, Cl27 cells, 3T3 cells, Chinese hamster ovary (CHO) cells, or derivatives thereof and related cell lines grown in serum-free medium, HeLa cells, BHK cell lines, CV-1EBNA cell lines, human embryonic kidney (HEK) cells (such as 293, 293EBNA or MSR 293), human epidermal A431 cells, human Colo205 cells, other transformed primate cell lines, normal diploid cells, cell lines derived from in vitro culture of primary tissues, primary explants, HL-60, U937, HaK or Jurkat cells. Optionally, when it is desired to use polypeptides in various signal transduction or reporter protein assays, mammalian cell lines (such as HepG2 / 3B, KB, NIH3T3 or S49) can be used for the expression of polypeptides. Alternatively, polypeptides can be produced in lower eukaryotes (such as yeast) or in prokaryotes (such as bacteria). Suitable yeasts include P. pastoris, S. cerevisiae, S. pombe, Kluyveromyces strains, Candida, or any yeast strain capable of expressing heterologous polypeptides. Suitable bacterial strains include Escherichia coli, B. subtilis, S. typhimurium, or any bacterial strain capable of expressing heterologous polypeptides. If the fusion protein is prepared in yeast or bacteria, it may be desirable to modify the product produced therein, for example, by phosphorylation or glycosylation of appropriate sites, in order to obtain a functional product. Such covalent attachment can be achieved using known chemical or enzymatic methods.

[0201] The method for introducing the expression vector containing the target polynucleotide sequence varies according to the type of cell host. For example, calcium chloride transfection is generally used for prokaryotic cells, and calcium phosphate treatment or electroporation can be used for other cell hosts. Other methods include, for example, electroporation, calcium phosphate treatment, liposome-mediated conversion, injection and microinjection, ballistic methods, virions, immunoliposomes, polycations: nucleic acid conjugates, naked DNA, artificial virions, fusion with herpes virus structural protein VP22, the uptake of DNA enhanced by medicaments and in vitro transduction. For the long-term high-yield production of recombinant proteins, stable expression is usually desired. For example, the expression vector of the present disclosure containing viral replication origin or endogenous expression element and selectable marker gene can be used to prepare a cell line stably expressing the engineered protein. After the introduction of the vector, cells can be grown in enriched medium for 1-2 days, and then they are converted to selective medium. The purpose of the selective marker is to confer selection resistance, and its presence allows the cell growth of the sequence introduced to be successfully expressed in selective medium. The tissue culture technique suitable for the cell type can be used to proliferate resistance, stable transfected cells.

[0202] Fusion proteins are typically recovered from the culture medium as secreted polypeptides, but can also be recovered from host cell lysates when they are produced directly without a secretion signal. If the polypeptide is membrane-bound, it can be released from the membrane using a suitable detergent solution (e.g., Triton-X 100).

[0203] When the fusion protein is produced in the recombinant cell of non-human cells, it does not contain human protein or polypeptide at all. However, it is necessary to purify the fusion protein from the recombinant cell protein or polypeptide. As a first step, the culture medium or lysate is usually centrifuged to remove granular cell debris. The molecules produced can be easily purified by hydroxyapatite chromatography, gel electrophoresis, dialysis or affinity chromatography, wherein affinity chromatography is a preferred purification technique. Other techniques for protein purification can also be used, such as fractionation on ion exchange columns, ethanol precipitation, reversed-phase HPLC, chromatography on silica, chromatography on heparin agarose, chromatography on anion or cation exchange resins (such as polyaspartic acid columns), chromatofocusing, SDS-PAGE and ammonium sulfate precipitation.

[0204] In some aspects, provided herein are viral vectors comprising polynucleotides encoding therapeutic fusion proteins of the present invention. In some embodiments, viral vectors are derived from AAV. In certain embodiments, viral vectors are administered to subjects, such as humans, in which therapeutic fusion proteins are expressed, and can be used to treat and / or prevent the diseases listed herein.

[0205] Pharmaceutical composition

[0206] On the other hand, the present disclosure provides a composition, such as a pharmaceutical composition, comprising a therapeutic fusion protein of the present invention and one or more pharmaceutically acceptable excipients, diluents or carriers. Such a composition may include one of the therapeutic fusion proteins of the present disclosure or a combination of therapeutic fusion proteins of the present disclosure (e.g., two or more different therapeutic fusion proteins).

[0207] The pharmaceutical compositions described herein can also be administered in combination therapy, i.e., in combination with other agents. For example, a combination therapy can include a fusion protein of the present disclosure in combination with, for example, at least one anti-inflammatory agent, anti-infective agent, or immunosuppressant. Examples of therapeutic agents that can be used in combination therapy are described in more detail below in the section on the use of therapeutic fusion proteins of the present disclosure.

[0208] To prepare a pharmaceutical or sterile composition comprising a fusion protein of the disclosure, the fusion protein is mixed with a pharmaceutically acceptable carrier or excipient.

[0209] The term 'pharmaceutically acceptable' means approved by a regulatory agency of the Federal or a state government or listed in the US Pharmacopeia or other generally recognized pharmacopeia for use in animals, and more particularly in humans.

[0210] The term 'pharmaceutical composition' refers to a mixture of at least one active ingredient (eg, an engineered protein) and at least one pharmaceutically acceptable excipient, diluent or carrier.

[0211] 'Drug' is a substance used in medical treatment.

[0212] As used herein, 'pharmaceutically acceptable carriers' include any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents, etc. that are physiologically compatible. The carrier should be suitable for intravenous, intramuscular, subcutaneous, parenteral, spinal or epidermal administration (e.g., by injection or infusion). In one embodiment, the carrier should be suitable for subcutaneous routes. Depending on the route of administration, the active compound (i.e., fusion protein) can be coated in a material to protect the compound from the action of acids and other natural conditions that may inactivate the compound.

[0213] The pharmaceutical compositions described herein may include one or more pharmaceutically acceptable salts. The pharmaceutical compositions described herein may also include a pharmaceutically acceptable antioxidant. Examples of pharmaceutically acceptable antioxidants include: water-soluble antioxidants, such as ascorbic acid, cysteine ​​hydrochloride, sodium bisulfate, sodium pyrosulfite, sodium sulfite, etc.; oil-soluble antioxidants, such as ascorbyl palmitate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), lecithin, propyl gallate, α-tocopherol, etc.; and metal chelators, such as citric acid, ethylenediaminetetraacetic acid (EDTA), sorbitol, tartaric acid, phosphoric acid, etc.

[0214] Examples of suitable aqueous and non-aqueous carriers that can be used in the pharmaceutical compositions described herein include water, ethanol, polyols (such as glycerol, propylene glycol, polyethylene glycol, etc.), and suitable mixtures thereof, vegetable oils (such as olive oil), and injectable organic esters (such as ethyl oleate). Proper fluidity can be maintained, for example, by the use of coating materials (such as lecithin), by maintaining the required particle size in the case of dispersions, and by the use of surfactants.

[0215] These compositions can also contain adjuvants such as preservatives, wetting agents, emulsifiers and dispersants. The presence of microorganisms can be prevented by sterilization procedures and by including various antibacterial and antifungal agents (e.g., parabens, chlorobutanol, phenol sorbic acid, etc.). It is also desirable to include isotonic agents such as sugar, sodium chloride, etc. in the composition. In addition, the extended absorption of injectable drug forms can be achieved by including agents that delay absorption (e.g., aluminum monostearate and gelatin).

[0216] Pharmaceutically acceptable carriers include sterile aqueous solutions or dispersions, and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. The use of such media and agents as pharmaceutically active substances is known in the art. Unless any conventional media or agents are incompatible with the active compound, it is contemplated that they will be used in the pharmaceutical compositions of the present invention. Supplementary active compounds may also be incorporated into the composition.

[0217] Therapeutic compositions typically must be sterile and stable under manufacturing and storage conditions. Compositions can be formulated as solutions, microemulsions, liposomes or other ordered structures suitable for high drug concentrations. The carrier can be a solvent or dispersion medium, and the solvent or dispersion medium contains, for example, water, ethanol, polyols (such as glycerol, propylene glycol and liquid polyethylene glycol, etc.) and mixtures suitable therefor. For example, suitable fluidity can be maintained by using a coating (such as lecithin), by maintaining the required particle size in the case of dispersions, and by using a surfactant. In many cases, isotonic agents, such as sugar, polyols (such as mannitol, sorbitol) or sodium chloride can be included in the composition.

[0218] Reviews on the development of stable protein formulations can be found in Cleland et al. (1993) Crit Reviews Ther Drug Carrier Systems, 10(4):307-377 and Wei W (1999) Int J Pharmaceutics, 185:129-88.

[0219] Solutions or suspensions used for intradermal or subcutaneous application typically include one or more of the following components: a sterile diluent, such as water for injection, saline solution, fixed oils, polyethylene glycol, glycerol, propylene glycol or other synthetic solvents; antibacterial agents, such as benzyl alcohol or methyl parabens; antioxidants, such as ascorbic acid or sodium bisulfite; chelating agents, such as ethylenediaminetetraacetic acid; buffers, such as acetates, citrates or phosphates; and agents for adjusting osmotic pressure, such as sodium chloride or dextrose. The pH can be adjusted with acids or bases, such as hydrochloric acid or sodium hydroxide. These preparations can be packaged in ampoules, disposable syringes, or multiple dose vials made of glass or plastic.

[0220] Sterile injectable solutions can be prepared in the following manner: optionally, the active compound is incorporated into a suitable solvent containing one or a combination of the above-listed components in the desired amount, followed by sterilization microfiltration. In general, dispersions are prepared by incorporating the fusion protein of the present invention into a sterile vehicle containing a basic dispersion medium and other components required from the above listing. In the case of sterile powders for the preparation of sterile injectable solutions, the preparation method is vacuum drying and freeze drying (lyophilization), which produces a powder of the active ingredient plus any other desired component from a previously sterile filtered solution of the active ingredient plus any other desired component.

[0221] The amount of active ingredient that can be combined with a carrier material to produce a single dosage form will vary depending on the subject being treated and the specific mode of administration. The amount of active ingredient that can be combined with a carrier material to produce a single dosage form is generally the amount of the composition that produces a therapeutic effect. Typically, within the range of one hundred percent, this amount will range from about 0.01% to about 99% active ingredient, from about 0.1% to about 70%, or from about 1% to about 30% active ingredient combined with a pharmaceutically acceptable carrier.

[0222] Selecting an administration regimen for a therapeutic engineered protein depends on several factors, including the serum or tissue turnover rate of the entity, the symptom level, the immunogenicity of the entity, and the target cell accessibility in the biomatrix. In certain embodiments, the administration regimen maximizes the amount of the therapeutic agent delivered to the patient consistent with an acceptable level of side effects. Therefore, the amount of the protein delivered depends in part on the severity of the specific entity and the disease being treated. Guidance for selecting appropriate doses of biologics and small molecules is available (see, e.g., Bach (ed.) (1993) Monoclonal Antibodies and Peptide Therapy in Autoimmune Diseases, Marcel Dekker, New York, NY; Baert, et al. (2003) New Engl. J. Med. 348:601-608; Milgrom, et al. (1999) New Engl. J. Med. 341:1966-1973; Slamon, et al. (2001) New Engl. J. Med. 344:783-792; Beniaminovitz, et al. (2000) New Engl. J. Med. 345:783-792). Engl. J. Med. 342:613-619; Ghosh, et al. (2003) New Engl. J. Med. 348:24-32; Lipsky, et al. (2000) New Engl. J. Med. 343:1594-1602).

[0223] The appropriate dosage is determined by the clinician, for example, using parameters or factors known in the art or suspected to affect treatment or expected to affect treatment. Typically, the dosage is started with an amount slightly less than the optimal dosage and is increased in small increments thereafter until the desired or optimal effect is achieved relative to any adverse side effects. Important diagnostic quantities include those of symptoms (e.g., inflammation) or the levels of inflammatory cytokines produced.

[0224] The actual dosage level of the active ingredient in the pharmaceutical composition of the present disclosure can be varied so as to obtain an amount of active ingredient that is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration without being toxic to the patient. The selected dosage level will depend on various pharmacokinetic factors, including the activity of the particular composition of the present disclosure employed, the route of administration, the time of administration, the excretion rate of the particular compound employed, the duration of treatment, other drugs, compounds, and / or materials combined with the particular composition employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and similar factors known in the medical field.

[0225] The dosage regimen is adjusted to provide the best desired response. For example, as indicated by the exigencies of the therapeutic situation, a single bolus may be administered, several divided doses may be administered over time, or the dose may be reduced or increased proportionally. It may be particularly advantageous to formulate parenteral compositions in unit dosage form for ease of administration and to achieve uniformity of dosage. As used herein, a unit dosage form refers to a physically discrete unit suitable for use as a single dose in a subject to be treated; each unit contains a predetermined amount of active compound calculated to produce the desired therapeutic effect and the required pharmaceutical carrier. The specifications of the dosage unit form of the present invention are specified by and directly depend on the unique characteristics of the active compound and the specific therapeutic effect to be achieved, as well as the inherent limitations in the field of compounding such active compounds for treating individual sensitivities.

[0226] For the administration of therapeutic fusion proteins, dosage is in the range of about 0.0001 to 150 mg / kg host body weight, such as 5, 15 and 50 mg / kg for subcutaneous administration, and more typically 0.01 to 5 mg / kg host body weight. Exemplary treatment regimens require administration once a week, once every two weeks, once every three weeks, once every four weeks, once a month, once every 3 months, or once every three to 6 months.

[0227] Therapeutic fusion protein of the present invention can be used in many cases. The interval between single doses can be, for example, weekly, monthly, every three months or annually. As shown by measuring the blood level of engineered protein in the patient, the interval can also be irregular. In some methods, the dosage is adjusted to obtain a plasma protein concentration of about 1-1000 μg / ml and about 25-300 μg / ml in some methods.

[0228] Alternatively, therapeutic fusion protein can be used as sustained release formulations, in which case less frequent administration is required. Dosage and frequency vary depending on the half-life of the protein in the patient's body, and can vary depending on whether the treatment is preventive or therapeutic. In preventive applications, relatively low dosages are administered at relatively infrequent intervals over a long period of time. Some patients can continue to receive treatment throughout their lives. In therapeutic applications, relatively high dosages are sometimes required to be administered at relatively short intervals until the disease or disease progression is reduced or terminated, or until the patient shows partial or complete improvement of the disease or disease symptoms. Thereafter, preventive regimens can be administered to the patient.

[0229] The actual dosage level of the active ingredient in the pharmaceutical composition of the present invention can be varied so as to obtain an amount of active ingredient that is effective to achieve the desired therapeutic response for a particular patient, composition, and mode of administration without being toxic to the patient. The selected dosage level depends on a variety of pharmacokinetic factors, including the activity of the particular composition of the disclosure employed, the route of administration, the time of administration, the excretion rate of the particular compound employed, the duration of treatment, other drugs, compounds, and / or materials combined with the particular composition employed, the age, sex, weight, condition, general health and prior medical history of the patient being treated, and similar factors known in the medical field.

[0230] A 'therapeutically effective dose' of the fusion protein of the invention may result in a reduction in severity of a disorder or symptom or disease and / or prevent impairment or disability resulting from the disorder.

[0231] The compositions of the present disclosure can be administered by one or more routes of administration using one or more of various methods known in the art. As will be appreciated by those skilled in the art, the administration route and / or mode will vary according to the desired result. The route of administration for the engineered protein of the present invention includes intravenous, intramuscular, intradermal, intraperitoneal, subcutaneous, spinal or other parenteral administration routes, such as by injection or infusion. As used herein, the phrase 'parenteral administration' means the mode of administration other than intestinal and topical administration, usually administered by injection, and includes but is not limited to intravenous, intramuscular, intraarterial, intrathecal, intracapsular, intraorbital, intracardiac, intradermal, intraperitoneal, transtracheal, subcutaneous, subcutaneous, intraarticular, subcapsular, subarachnoid, intraspinal, extradural and intrasternal injection and infusion.

[0232] Alternatively, the therapeutic fusion proteins of the invention can be administered via non-parenteral routes, such as topical, epidermal or mucosal administration routes.

[0233] The therapeutic fusion proteins disclosed herein can be prepared with carriers that will prevent the rapid release of the protein, such as controlled release formulations, including implants, transdermal patches, and microencapsulated delivery systems. Biodegradable biocompatible polymers such as ethylene vinyl acetate, polyanhydrides, polyglycolic acid, collagen, polyorthoesters, and polylactic acid can be used. Methods for preparing such formulations are patented or generally known to those skilled in the art. See, for example, Sustained and Controlled Release Drug Delivery Systems, edited by JR Robinson, Marcel Dekker, Inc., New York, 1978.

[0234] In certain embodiments, the therapeutic fusion proteins of the present invention can be formulated to ensure proper in vivo distribution. For example, the blood-brain barrier (BBB) ​​excludes many highly hydrophilic compounds. In order to ensure that the therapeutic compounds of the present invention pass through the BBB (if necessary), they can be formulated in, for example, liposomes. For methods of making liposomes, see, for example, U.S. Patents 4,522,811; 5,374,548; and 5,399,331. Liposomes can include one or more parts that are selectively transported to specific cells or organs, thereby enhancing targeted drug delivery (see, for example, Ranade VV (1989) J. Clin. Pharmacol. [Journal of Clinical Pharmacology], 29: 685).

[0235] Therapeutic uses and methods of the invention

[0236] The therapeutic fusion proteins of the present invention have in vitro and in vivo diagnostic and therapeutic uses. For example, these molecules can be administered to cells in culture (e.g., in vitro) or in a subject (e.g., in vivo) to treat, prevent or diagnose a variety of disorders. The methods are particularly suitable for treating, preventing or diagnosing acute or chronic inflammatory and immune system-driven organ and microvascular disorders.

[0237] The therapeutic fusion proteins of the present invention can be used for, but are not limited to, the treatment, prevention or improvement of acute and chronic inflammatory organ damage, particularly inflammatory damage in which endogenous homeostatic clearance mechanisms or efferocytosis pathways for the removal of dying cells, cell debris and pro-thrombotic / pro-inflammatory microparticles are significantly downregulated. Examples of acute inflammatory organ damage include myocardial infarction, acute kidney injury (AKI), acute stroke and inflammation, and organ damage caused by ischemia / reperfusion, such as ischemia / reperfusion of the gastrointestinal tract, liver, spleen, lung, kidney, pancreas, heart, brain, spinal cord and / or crushed limbs.

[0238] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or improve inhibition or slowing of blood coagulation, microbiome therapy, inflammatory bowel disease (IBD), fatty acid uptake and / or reduced gastric motility, microthrombosis-dependent disorders, atherosclerosis, cardiac remodeling, tissue fibrosis, acute liver injury, chronic liver disease, non-alcoholic steatohepatitis (NASH), vascular disease, age-related vascular disorders, intestinal disease, sepsis, bone disorders, cancer, thalassemia, pancreatitis, hepatitis, endocarditis, pneumonia, acute lung injury, osteoarthritis, periodontitis, inflammation caused by tissue trauma, colitis, diabetes, hemorrhagic shock, transplant rejection, radiation-induced damage, splenomegaly, AKI or multiple organ failure caused by sepsis, acute burns, adult respiratory distress syndrome, wound healing, tendon repair and neurological diseases.

[0239] In one embodiment, the neurological disease can be selected from disorders with neuropsychiatric, neuroinflammatory and / or neurodegenerative components, which include symptoms such as sickness syndrome, nausea, passive avoidance, inhibition of behavioral agility, memory impairment and memory dysfunction. Examples of neurological diseases include neurological diseases associated with amyloid beta, such as Alzheimer's disease, Parkinson's disease and depression.

[0240] In one embodiment, bone disorders can be selected from the diseases including osteoporosis, osteomalacia, osteosclerosis and osteopetrosis. More particularly, the administration of the fusion protein disclosed herein can inhibit the expression of at least one osteoclast marker such as NFATc1, cathepsin K and αvβ3 integrin. In one embodiment, the administration inhibits osteoclastogenesis. In another embodiment, the administration inhibits RANKL-induced osteoclastogenesis. In another embodiment, the administration inhibits bone resorption. In another embodiment, the administration inhibits the expression of at least one bone resorption stimulator (e.g., bone resorption stimulators comprising the following: TNF, IL-6, IL-17A, MMP-9, Ptgs2, RANKL, Tnfsf11, CXCL1, CXCL2, CXCL3, CXCL5 and combinations thereof). In another embodiment, the administration inhibits the expression of at least one proinflammatory cytokine selected from the group consisting of IL-8 and CCL2 / MCP-1.

[0241] In one embodiment, tissue fibrosis can be fibrosis in the liver, lung, diaphragm, kidney, brain, heart, wherein the fusion protein of the present invention reduces collagen expression. In one embodiment, pulmonary fibrosis is interstitial pulmonary fibrosis (IPF). In one embodiment, liver fibrosis is cirrhosis, which may or may not be attributed to NASH.

[0242] Many respiratory diseases are characterized by the accumulation of apoptotic cells. In addition, defective efferocytosis and phagocytosis of macrophages in chronic obstructive pulmonary disease (COPD) are associated with exacerbation and severity of the disease. The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or improve respiratory diseases, such as acute respiratory distress syndrome or COPD. The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or improve: acute lung injury (ALI), such as lung injury caused by inhalation or inhalation of toxic exogenous or endogenous compounds or drugs; lung injury caused by pulmonary edema, shock, pancreatitis, burns, chest trauma or multiple trauma, radiation, sepsis, pathogens (bacteria, viruses or parasites such as Plasmodium); chronic lung insufficiency diseases that lead to hypoxemia

[0243] The therapeutic fusion protein disclosed herein can also be used to diagnose, treat, prevent or improve the severity of lung damage caused by a coronavirus (e.g., SARS-CoV, SARS-CoV-2 or MERS-CoV). In one embodiment, the therapeutic fusion protein of the present invention is provided for treating SARS-CoV-2 infection in COVID 19 patients.

[0244] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or ameliorate the severity of transfusion-related pulmonary insufficiency (TRALI).

[0245] The therapeutic fusion protein disclosed herein can also be used to diagnose, treat, prevent or improve the severity of chronic pulmonary insufficiency diseases that cause hypoxemia.

[0246] The therapeutic fusion proteins disclosed herein (eg, therapeutic fusion proteins comprising a domain of EDIL3 disclosed herein) can also be used to diagnose, treat, prevent, or improve the severity of postoperative peritoneal adhesions.

[0247] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or ameliorate the severity of heart failure.

[0248] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent, or improve the severity of hemodialysis.

[0249] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or ameliorate the severity of delayed graft function or graft-versus-host disease.

[0250] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or ameliorate the severity of severe frostbite, trench foot, pyoderma gangrenosum / gangrene.

[0251] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or ameliorate the severity of pathologies caused by bacteria, fungi, viruses or parasites (e.g., sepsis or other pathologies directly induced by pathogens, such as in anthrax, plague, necrotizing soft tissue infections (NSTIs, such as necrotizing fasciitis), osteomyelitis, malaria).

[0252] The therapeutic fusion proteins disclosed herein can also be used to diagnose, treat, prevent or ameliorate the severity of trauma / polytrauma caused by traumatic accidents (e.g., work accidents, falls, traffic accidents, bullet and combat injuries or other injury mechanisms).

[0253] The therapeutic fusion proteins of the present disclosure can also be used to diagnose, treat, prevent, or ameliorate the severity of osteoclast-mediated pathology.

[0254] The therapeutic fusion proteins disclosed herein can be administered as the sole active ingredient or in combination (e.g., as an adjuvant) or in combination with other drugs (e.g., immunosuppressants or immunomodulators or other anti-inflammatory agents or, for example, cytotoxic agents or anticancer agents), for example, to treat or prevent the above-mentioned diseases.

[0255] For administration of 'combination' with another therapeutic agent, it is meant that two (or more) different treatments are delivered to a subject during the course of the subject's disorder, for example, two or more treatments are delivered after the subject is diagnosed with the disorder and before the disorder is cured or eliminated or the treatment is terminated for other reasons. In some embodiments, the delivery of the first treatment is still ongoing when the delivery of the second treatment begins, so there is overlap in terms of administration. This is sometimes referred to as "simultaneous delivery" or "concurrent delivery" in this article. In other embodiments, the delivery of one treatment ends before the delivery of the other treatment begins. In some embodiments of each case, the treatment is more effective due to combined administration. For example, the second treatment is more effective, for example, an equivalent effect is observed with less of the second treatment, or the second treatment reduces symptoms to a greater extent, or similar conditions are observed for the first treatment, compared to the results observed when the second treatment is administered in the absence of the first treatment. In some embodiments, delivery reduces symptoms or other parameters associated with the disorder more than the results observed when the other treatment is delivered in the absence of one treatment. The effects of the two treatments may be partially additive, fully additive, or greater than additive. The delivery may be such that the effect of the first treatment delivered is still detectable when the second treatment is delivered.

[0256] The term 'concurrent' is not limited to administering therapies (e.g., prophylactic or therapeutic agents) at exactly the same time, but means that a pharmaceutical composition comprising a therapeutic fusion protein of the present disclosure is administered to a subject in a certain order and within a certain time interval so that the fusion protein can work together with one or more additional therapeutic agents to provide an increased benefit compared to if administered in another manner. For example, each therapy can be administered to a subject at the same time or sequentially in any order at different time points; however, if not administered at the same time, the therapy should be administered sufficiently close in time to provide the desired therapeutic or preventive effect. Each therapy can be administered to a subject separately in any appropriate form and by any suitable route.

[0257] The therapeutic fusion protein described herein and one or more additional therapeutic agents can be administered simultaneously (in a pharmaceutical composition identical or separate from the disclosed fusion protein) or sequentially. For sequential administration, the fusion protein described herein can be administered first, and then the additional agent can be administered, or the order of administration can be reversed. Compared to the fusion protein, one or more additional therapeutic agents can be administered to the subject by the same or different routes of administration.

[0258] Therapeutic fusion proteins as described herein and / or one or more additional therapeutic agents, procedures or methods can be administered during active disorders, or during remission or during less active diseases. Therapeutic fusion proteins as described herein can be administered before other treatments, concurrently with treatments, after treatments, or during disorder remissions.

[0259] When administered in combination, therapeutic fusion proteins described herein and additional therapeutic agents (e.g., second or third agents) or all are administered with higher, lower or the same amount or dosage than the amount or dosage of each agent used alone (e.g., as a monotherapy). In certain embodiments, therapeutic fusion proteins described herein, additional agents (e.g., second or third agents) or all are lower than the amount or dosage of each agent used alone (e.g., as a monotherapy) (e.g., at least 20%, at least 30%, at least 40%, or at least 50%). In other embodiments, the amount or dosage of therapeutic fusion proteins described herein, additional agents (e.g., second or third agents) or all of which result in the desired effect (e.g., treating inflammatory diseases or disorders) is lower than the amount or dosage required for achieving the same therapeutic effect of each agent used alone (e.g., as a monotherapy) (e.g., at least 20%, at least 30%, at least 40%, or at least 50%).

[0260] For example, the therapeutic fusion proteins of the present disclosure can be used in combination with the following drugs: DMARDs, such as gold salts, sulfasalazine, antimalarials, methotrexate, D-penicillamine, azathioprine, mycophenolic acid, tacrolimus, sirolimus, minocycline, leflunomide, glucocorticoids; calcineurin inhibitors, such as cyclosporine A or FK 506; regulators of lymphocyte recirculation, such as FTY720 and FTY720 analogs; mTOR inhibitors, such as rapamycin, 40-O-(2-hydroxyethyl)-rapamycin, CCI779, ABT578, AP23573 or TAFA-93; ascomycins with immunosuppressive properties, such as ABT-281, ASM981, etc.; corticosteroids; cyclophosphamide; azathioprine; leflunomide; mizoribine; mycophenolic acid morpholinoethyl ester; 15-deoxyspergualin or its Immunosuppressive analogs, analogs or derivatives; immunosuppressive monoclonal antibodies, such as monoclonal antibodies directed against leukocyte receptors, such as MHC, CD2, CD3, CD4, CD7, CD8, CD25, CD28, CD40, CD45, CD58, CD80, CD86 or their ligands; other immunomodulatory compounds, such as recombinant binding molecules having at least a portion of the extracellular domain of CTLA4 or a mutant thereof, such as CTLA4 with non-CTLA4 protein sequences such as CTLA4Ig (e.g., designated as ATCC 68629) or a mutant thereof, such as LEA29Y, or a mutant thereof; an adhesion molecule inhibitor, such as an LFA-1 antagonist, an ICAM-1 or ICAM-3 antagonist, a VCAM-4 antagonist, or a VLA-4 antagonist; or a chemotherapeutic agent, such as paclitaxel, gemcitabine, cisplatin, doxorubicin, or 5-fluorouracil; an anti-TNF agent, such as a monoclonal antibody to TNF, such as infliximab, adalimumab, CDP870, or Receptor constructs of TNF-RI or TNF-RII, such as Etanercept, PEG-TNF-RI; blockers of proinflammatory cytokines, IL-1 blockers, such as anakinra or IL-1 trap, canakinumab, IL-13 blockers, IL-4 blockers, IL-6 blockers; chemokine blockers, such as inhibitors or activators of proteases such as metalloproteinases, anti-IL-15 antibodies, anti-IL-6 antibodies, anti-IL-4 antibodies, anti-IL-13 antibodies, anti-CD20 antibodies, NSAIDs such as aspirin or anti-infective agents;Damage-associated molecular pattern (DAMP) or pathogen-associated molecular pattern (PAMP) antagonists, such as converters, detoxifiers, removers, such as ATP converters, HMGB-1 modulators, histone detoxifiers; inhibitors of superantigen-induced immune responses; complement inhibitors and extracorporeal plasma exchange devices. ;

[0261] Reagent test kit

[0262] Kits are also included within the scope of the present invention, and the kits are composed of, for example, the compositions and instructions for use of the therapeutic fusion proteins disclosed herein. Such kits include a therapeutically effective amount of fusion proteins according to the present disclosure. In addition, such kits may include tools (e.g., automatic syringes, syringes and vials, prefilled syringes, prefilled pens) and instructions for use for administering therapeutic fusion proteins. These kits may include additional therapeutic agents (as described below) for treating patients with autoimmune diseases or inflammatory disorders or AOI. Such kits may also include instructions for administering therapeutic fusion proteins to treat patients. Such instructions may provide dosages, routes of administration, regimens, and total treatment durations for the fusion proteins packaged. Kits typically include labels indicating the intended use of the contents of the kit. The term label includes any written or recorded material provided on the kit or provided with the kit. The kit may further include tools for diagnosing whether a patient belongs to a group of responses to the treatment of the therapeutic fusion proteins of the present invention as defined above.

[0263] Example

[0264] This disclosure provides the following embodiments:

[0265] 1. A therapeutic fusion protein for enhancing efferocytosis, the therapeutic fusion protein comprising an integrin binding domain, a phosphatidylserine (PS) binding domain and a solubilization domain, wherein the solubilization domain is inserted between the integrin binding domain and the PS binding domain, and wherein the PS binding domain is a truncated variant.

[0266] 2. The fusion protein of Example 1, wherein the PS binding domain is a truncated variant of at least one PS binding domain listed in Table 2.

[0267] 3. The fusion protein of embodiment 1 or embodiment 2, wherein the PS binding domain is a truncated variant of the PS binding motif of MFG-E8 or EDIL3.

[0268] 4. The fusion protein of Example 3, wherein the PS binding domain is a truncated variant of the PS binding motif of MFG-E8.

[0269] 5. The fusion protein of embodiment 4, wherein the PS binding domain is a Dictyostelium lectin domain.

[0270] 6. The fusion protein of any one of the preceding embodiments, wherein the PS binding domain is a C1 domain.

[0271] 7. The fusion protein of any one of the preceding embodiments, wherein the PS binding domain does not comprise a C2 domain.

[0272] 8. A fusion protein for enhancing efferocytosis, the fusion protein comprising an integrin binding domain, a phosphatidylserine (PS) binding domain and a solubilization domain, wherein the solubilization domain is inserted between the integrin binding domain and the PS binding domain, and wherein the PS binding domain is a C1 domain.

[0273] 9. The fusion protein of any one of the preceding embodiments, wherein the integrin binding domain binds to one or more integrins.

[0274] 10. The fusion protein of Example 9, wherein the integrin binding domain binds to αvβ3 and / or αvβ5 and / or α8β1 integrin.

[0275] 11. The fusion protein of any one of the preceding embodiments, wherein the integrin binding domain comprises an arginine-glycine-aspartic acid (RGD) motif.

[0276] 12. A therapeutic fusion protein of formula EGF-SC (Formula I), wherein

[0277] (i) EGF is an integrin binding domain, wherein the integrin binding domain binds to one or more proteins,

[0278] (ii) S is a solubilization domain, and

[0279] (iii) C is a truncated PS binding domain.

[0280] 13. The therapeutic fusion protein of Example 12, wherein the integrin binding domain binds to αvβ3 and / or αvβ5 and / or α8β1 integrin.

[0281] 14. The therapeutic fusion protein of embodiment 12 or embodiment 13, wherein the integrin binding domain comprises an arginine-glycine-aspartic acid (RGD) motif.

[0282] 15. The therapeutic fusion protein of any one of embodiments 12 to 14, wherein the integrin binding domain is an EGF-like domain of MFG-E8, EDIL3, or a protein comprising an integrin binding domain listed in Table 1.

[0283] 16. The therapeutic fusion protein of any one of embodiments 12 to 15, wherein the truncated PS binding domain is a truncated variant of the PS binding domain listed in Table 2.

[0284] 17. The therapeutic fusion protein of any one of embodiments 12 to 16, wherein the PS binding domain is a truncated variant of the PS binding motif of MFG-E8 or EDIL3.

[0285] 18. The fusion protein of any one of embodiments 12 to 16, wherein the PS binding domain is a truncated variant of the PS binding motif of MFG-E8.

[0286] 19. The fusion protein of any one of embodiments 12 to 18, wherein the PS binding domain is a Dictyostelium lectin domain.

[0287] 20. The therapeutic fusion protein of any one of embodiments 11 to 16, wherein the truncated PS binding domain comprises any one of the C1 domain and / or C2 domain of the PS binding domain listed in Table 2.

[0288] 21. The therapeutic fusion protein of any one of embodiments 11 to 18, wherein the truncated PS binding domain is a C1 domain.

[0289] 22. The therapeutic fusion protein of any one of embodiments 11 to 21, wherein the truncated PS binding domain does not comprise a C2 domain.

[0290] 23. The fusion protein of any one of the preceding embodiments, wherein the solubilizing domain is directly linked to the integrin binding domain, to the PS binding domain, or to both domains.

[0291] 24. The fusion protein of any one of the preceding embodiments, wherein the solubilization domain is indirectly linked to the integrin binding domain and / or the PS binding domain via a linker.

[0292] 25. The fusion protein of any preceding embodiment, wherein the integrin binding domain has the amino acid sequence of SEQ ID NO: 2 or has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2.

[0293] 26. The fusion protein of any preceding embodiment, wherein the integrin binding domain has the amino acid sequence of SEQ ID NO: 77 or has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 77.

[0294] 27. The fusion protein of any of the preceding embodiments, wherein the integrin binding domain has an amino acid sequence selected from, or has at least 90% sequence identity to, an amino acid sequence selected from SEQ ID NO:96, SEQ ID NO:97, SEQ ID NO:98, SEQ ID NO:99, SEQ ID NO:100, or SEQ ID NO:101.

[0295] 28. The fusion protein of any one of the preceding embodiments, wherein the PS binding domain has an amino acid sequence of SEQ ID NO: 141 or SEQ ID NO: 142 or has at least 90% sequence identity to an amino acid sequence of SEQ ID NO: 141 or SEQ ID NO: 142.

[0296] 29. The fusion protein of any one of the preceding embodiments, wherein the PS binding domain has the amino acid sequence of SEQ ID NO: 144 or has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 144.

[0297] 30. The fusion protein of any preceding embodiment, wherein the solubilizing domain is HSA and has an amino acid sequence of SEQ ID NO: 4 or has at least 90% sequence identity to an amino acid sequence of SEQ ID NO: 4.

[0298] 31. The fusion protein of any one of the preceding embodiments, comprising, in sequence: an integrin binding domain-an HSA-PS binding domain.

[0299] 32. A therapeutic fusion protein comprising MFG-E8 and a solubilization domain, wherein the MFG-E8 comprises, from N-terminus to C-terminus: an EGF-like domain, a C1 domain or a C2 domain, and comprises a sequence from wild-type human MFG-E8 (SEQ ID NO: 1) or a functional variant thereof.

[0300] 33. A fusion protein as described in Example 32, wherein the solubilization domain is inserted between the EGF-like domain and the C1 or C2 domain.

[0301] 34. The fusion protein of any one of the preceding embodiments, wherein the solubilizing domain is HSA, HSA D3 or Fc-IgG or a functional variant thereof.

[0302] 35. The fusion protein of any one of the preceding embodiments, wherein the solubilizing domain comprises human serum albumin (HSA) or a functional variant thereof.

[0303] 36. A fusion protein, wherein the fusion protein has the amino acid sequence of SEQ ID NO: 34 or has at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 34.

[0304] 37. A fusion protein, wherein the fusion protein has the amino acid sequence of SEQ ID NO: 36 or has at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 36.

[0305] 38. A fusion protein, wherein the fusion protein has an amino acid sequence selected from the group consisting of SEQ ID NO: 119, SEQ ID NO: 121, SEQ ID NO: 125, SEQ ID NO: 129, SEQ ID NO: 131, SEQ ID NO: 133, SEQ ID NO: 135, SEQ ID NO: 137, or SEQ ID NO: 147.

[0306] 39. A fusion protein, wherein the fusion protein has the amino acid sequence of SEQ ID NO: 147 or has at least 90% sequence identity with the amino acid sequence of SEQ ID NO: 147.

[0307] 40. A fusion protein as described in any one of the preceding embodiments, wherein the fusion protein

[0308] a. Restoring impaired efferocytosis of macrophages in a human macrophage-neutrophil efferocytosis assay;

[0309] b. reducing the number of plasma microparticles by clearance in a human endothelial cell-microparticle efferocytosis assay; and / or

[0310] c. Prevents multiple organ damage in an acute renal ischemia model.

[0311] d. Improved disease burden in liver fibrosis models

[0312] 41. An isolated nucleic acid encoding the amino acid sequence of any one of embodiments 36 to 39.

[0313] 42. A cloning vector or an expression vector comprising the nucleic acid described in Example 41.

[0314] 43. A viral vector comprising the isolated nucleic acid as described in Example 41, preferably, the viral vector comprising the isolated nucleic acid as described in Example 41 is derived from AAV.

[0315] 44. The viral vector of embodiment 43, wherein the vector is administered to a subject in need thereof, e.g., a human subject.

[0316] 45. The viral vector of Example 43, for use in the treatment and / or prevention of the diseases listed herein.

[0317] 46. ​​A recombinant host cell suitable for producing a therapeutic fusion protein, the recombinant host cell comprising one or more cloning vectors or expression vectors as described in embodiment 42, and optionally a secretion signal.

[0318] 47. The recombinant host cell of embodiment 46, wherein the host cell is, for example, a prokaryotic, yeast, insect, or mammalian cell.

[0319] 48. The fusion protein of any one of the preceding embodiments, wherein expression of the protein in a host cell results in a yield of at least 10 mg / L.

[0320] 49. The fusion protein of any one of the preceding embodiments, wherein expression of the protein in mammalian cells results in at least a 100-fold increase in yield compared to wild-type MFG-E8 (SEQ ID NO: 1).

[0321] 50. A pharmaceutical composition comprising the fusion protein of any one of embodiments 1 to 40 and at least one pharmaceutically acceptable carrier.

[0322] 51. A method of treating or preventing an inflammatory disorder or inflammatory organ damage in a subject in need thereof, the method comprising administering to the subject a therapeutically effective amount of the fusion protein of any one of embodiments 1 to 40.

[0323] 52. The fusion protein of any one of embodiments 1 to 40, for use in treating or preventing an inflammatory disorder or inflammatory organ damage in a subject in need thereof.

[0324] 53. The method of embodiment 51 or the use of embodiment 52, wherein the inflammatory disorder or inflammatory organ damage is acute kidney injury, sepsis, myocardial infarction, acute stroke, burns, trauma, and inflammatory and organ damage caused by ischemia / reperfusion.

[0325] 54. The method of embodiment 51 or the use of embodiment 52, wherein the fusion protein is administered in combination with another therapeutic agent.

[0326] 55. The method or use of embodiment 54, wherein the another therapeutic agent is an immunosuppressant, an immunomodulator, an anti-inflammatory agent, an antioxidant, an anti-infective agent, a cytotoxic agent, or an anti-cancer agent.

[0327] 56. A therapeutic fusion protein comprising MFG-E8 and a solubilization domain, wherein the MFG-E8 comprises, from N-terminus to C-terminus: an EGF-like domain, a C1 domain or a C2 domain, and comprises a functional variant of the sequence from wild-type human MFG-E8 (SEQ ID NO: 1).

[0328] 57. The fusion protein of embodiment 56, wherein the solubilization domain is inserted between the EGF-like domain and the C1 domain.

[0329] 58. A fusion protein as described in Example 56, wherein the solubilization domain is inserted between the EGF-like domain and the C2 domain.

[0330] 59. A fusion protein as described in any one of embodiments 56 to 58, wherein the solubilizing domain is HSA, HSAD3 or Fc-IgG or a functional variant thereof.

[0331] 60. The fusion protein of any one of embodiments 56 to 59, wherein the fusion protein has an amino acid sequence selected from the SEQ IDs listed in Table 4.

[0332] 61. An isolated nucleic acid encoding the fusion protein of any one of embodiments 56 to 60.

[0333] 62. A viral vector comprising the isolated nucleic acid as described in Example 61, preferably, the viral vector comprising the isolated nucleic acid as described in Example 60 is derived from AAV.

[0334] 63. The viral vector of embodiment 62, wherein the vector is administered to a subject in need thereof, e.g., a human subject.

[0335] 64. The viral vector of Example 62, for use in treating and / or preventing the diseases listed herein.

[0336] 65. A cloning vector or expression vector comprising the nucleic acid described in Example 61.

[0337] 66. A recombinant host cell suitable for producing a therapeutic fusion protein, the recombinant host cell comprising one or more cloning vectors or expression vectors as described in embodiment 65, and optionally a secretion signal.

[0338] 67. The recombinant host cell of embodiment 66, wherein the host cell is, for example, a prokaryotic, yeast, insect or mammalian cell.

[0339] 67. The fusion protein of any one of embodiments 56 to 60, wherein expression of the protein in a host cell results in a yield of at least 10 mg / L.

[0340] 69. The fusion protein of any one of embodiments 56 to 60, wherein expression of the protein in mammalian cells results in a yield that is at least 100-fold increased compared to wild-type MFG-E8.

[0341] It should be understood that each embodiment can be combined with one or more other embodiments until such combination is consistent with the description of the embodiment. It should also be understood that the embodiments provided above should be understood to include all embodiments, including such embodiments generated by the combination of embodiments.

[0342] All references cited herein, including patents, patent applications, articles, publications, textbooks, etc., and references cited therein, are hereby incorporated by reference in their entirety to the extent they are not already incorporated.

[0343] Examples

[0344] The following examples are provided to further illustrate the present disclosure, but do not limit the scope of the present disclosure. Other variations of the present disclosure will be apparent to those of ordinary skill in the art and are also covered by the appended claims.

[0345] Example 1: Production of fusion proteins

[0346] MFG-E8 is a multidomain protein consisting of an N-terminal epidermal growth factor (EGF-like) domain and two C-terminal lectin C-type domains (C1 and C2). Attempts to produce recombinant full-length human protein as documented in the literature have shown protein aggregation and very low expression rates (Castellanos et al., (2016) Protein Expression Purification 1124: 10-22). Therefore, in an attempt to solubilize the protein and increase its expression, we investigated the effects of fusing various proteins to MFG-E8.

[0347] The solubilization domain (SD) from human Fc-IgG1, human serum albumin (HSA), and domain 3 of HSA (HSA D3) were fused to MFG-E8 at different positions; e.g. Figure 1 As shown, at the N or C terminus, or between EGF and C1 or C1 and C2 domains. In addition, fusion with Fc-IgG1 or HSA has the potential to extend the half-life of the molecule in vivo because these proteins bind to FcRn. Fusion of MFG-E8 with Fc-IgG1 or HSA can also improve the yield and solubility of the fusion protein (Castellanos et al., (2016) supra), as shown in the following examples.

[0348] Table 5 shows the fusion of the fusion protein FP330 (EGF-HSA-C1-C2; SEQ ID NO: 42) comprising an HSA insert to the human neonatal Fc receptor (see also Example 5.1).

[0349] Table 5: Binding affinity of fusion protein FP330 to human FcRn

[0350]

[0351] Example 2: Generation of wtMFG-E8 and MFG-E8 HSA fusions; expression and purification

[0352] Methods for producing fusion proteins are described below; briefly, MFG-E8 and MFG-E8 fusions and EDIL fusions, particularly fusions with HSA, were produced according to the following methods.

[0353] DNA was synthesized at GeneArt (Regensburg, Germany) and cloned into mammalian expression vectors using restriction enzyme-ligated cloning techniques. The resulting plasmid was transfected into HEK293T cells. In order to transiently express protein, polyethyleneimine (PEI; Catalog No. 24765, Polysciences, Inc.) was used to transfect the vector for wild-type or engineered chains into HEK293T cells adapted to suspension. Typically, 100 ml cells suspended at a density of 1-2 Mio cells / ml were transfected with DNA containing 100 μg of expression vectors encoding engineered chains. The recombinant expression vector was then introduced into the host cell, and constructs were produced by further culturing the cell for 7 days to allow secretion into a culture medium (HEK, serum-free medium) supplemented with 0.1% pluronic acid, 4 mM glutamine, and 0.25 μg / ml antibiotics.

[0354] The resulting constructs were then purified from the cell-free supernatant using immobilized metal ion affinity chromatography (IMAC), protein A capture, or anti-HSA capture chromatography.

[0355] When IMAC captures the histidine-tagged protein, the filtered conditioned medium is mixed with IMAC resin (GE Healthcare) and equilibrated with 1% triton and 20 mM NaPO4, 0.5 Mn NaCl, 20 mM imidazole (pH 7.0). The resin is washed three times with 15 column volumes of 20 mM NaPO4, 0.5 Mn NaCl, 20 mM imidazole (pH 7.0), and the protein is eluted with 10 column volumes of elution buffer (20 mM NaPO4, 0.5 Mn NaCl, 500 mM imidazole (pH 7.0)).

[0356] When proteins were captured by protein A or anti-HSA chromatography, the filtered conditioned media was mixed with protein A resin (CaptivA PriMab TM , Repligen) or anti-HSA resin (Capture Select Human Albumin affinity matrix, Thermo) was mixed and equilibrated with PBS (pH 7.4). The resin was washed three times with 15 column volumes of PBS (pH 7.4), and then the protein was eluted with 10 column volumes of elution buffer (50 mM citrate, 90 mM NaCl (pH 2.5)) and the pH was neutralized with 1 M TRIS pH 10.0.

[0357] Finally, the eluted fractions were polished by using size exclusion chromatography (HiPrep Superdex 200, 16 / 60, GE Healthcare Life Sciences) and analyzed by SDS-PAGE against PrecisionPlus Protein Unstained Standard Marker (Biorad, ref#161-0363).

[0358] Representative expression gel of fusion protein Figure 2 As shown: Figure 2 A: EGF-HSA-C1-C2 protein (FP330; SEQ ID NO: 42); Figure 2 B: EGF-HSA-C1-C2 of EDIL3 protein (FP050; SEQ ID NO: 12); Figure 2C: Unreduced and reduced EGF-Fc (KiH) C1-C2 protein. This protein is a heterodimer of FP071 (EGF-Fc (knob) -C1-C2; SEQ ID NO: 18) and Fc-IgG1 hole (SEQ ID NO: 10); Figure 2 D: EGF-HSA-C1 protein (FP260; SEQ ID NO: 34). The protein under reducing and non-reducing conditions was Figure 2 C, because the heterodimer tends to dissociate under reducing conditions, two conditions were tested. Table 6 shows the results of expression and yield after another set of fusion protein purification; from the expression data, it can be seen that the HSA fusion of MFG-E8 (even if fused to HSA at a different position) is expressed at least 100 times higher than wtMFG-E8. As shown in the right column of Table 6, the HSA fusion of MFG-E8 also shows at least a 100-fold increase in yield over wtMFG-E8.

[0359] Table 6: Expression and yield of fusion proteins expressed in HEK cell lines

[0360]

[0361] Other examples of therapeutic fusion proteins of the present disclosure were produced according to the above methods and further analyzed by SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis), which separates proteins based on their molecular weight. Each protein was mixed with Laemmli buffer and then loaded onto a polyacrylamide gel (Bio-Rad, 4%-20% Mini-PROTEAN TGX stain-free). After migration in TRIS-glycine-SDS running buffer at 200V for 30 minutes, the proteins contained in the gel were visualized in a stain-free imager (Bio-Rad, Gel Doc EZ). Figure 2 As shown in E, SDS-PAGE shows the recombinant protein that has been produced and purified:

[0362]

[0363] Example 3: Characterization of MFG-E8-HSA Engineered Protein

[0364] 3.1 Phosphatidylserine binding (biochemistry)

[0365] L-α-phosphatidylserine (brain, porcine, Avanti 840032, Alabama, USA) was dissolved in chloroform, diluted with methanol, and coated at 1 μg / mL in 384-well microtiter plates (Corning TM3653, Kennebunk, Maine, USA). After incubation at 4°C overnight, the cells were evacuated using a SpeedVac TM System (Thermo Scientific TM ) The solvent was evaporated. The plates were treated with 3% fatty acid-free bovine serum albumin (BSA) in phosphate buffered saline (PBS) for 1.5 hours at room temperature.

[0366] Binding of the fusion protein to L-α-phosphatidylserine was assessed by competition with biotinylated murine MFG-E8 / lactamase (generated in-house, mMFG-E8:biotin). The protein was diluted in PBS (pH 7.4) containing 3% fatty acid-free BSA and incubated with L-α-phosphatidylserine-coated microtiter plates for 30 min. mMFG-E8:biotin in PBS (pH 7.4) containing 3% fatty acid-free BSA was added at 1 nM and incubated for an additional 30 min. The chromatin was expressed as 100 ng / mL by dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA TM ) Wash Buffer (Perkin Elmer 1244-114MA, USA) was used to remove unbound mMFG-E8:biotin by three washing steps. TM Europium-labeled streptavidin (PerkinElmer 1244-360, Wallac Oy, Finland) was added to the assay buffer (PerkinElmer 1244-111MA, USA) and allowed to stand for 20 minutes. TM Three washing steps were performed with the assay buffer. Europium was revealed according to the manufacturer's instructions (PerkinElmer 1244-105, Boston, MA, USA). Time-resolved fluorescence of europium was determined by Envision TM Quantification was performed using a 2103 multi-label microplate reader (PerkinElmer, Connecticut, USA). Data were analyzed using MS Excel and GraphPad Prism software.

[0367] Polypropylene plates are low protein binding microtiter plates typically used in the laboratory for serial dilutions. These plates have the advantage of reduced protein loss during dilution compared to polystyrene and are typically classified as "low protein binding" plates. When dilutions of wtMFG-E8 were prepared in polypropylene plates, wtMFG-E8 lost potency in the L-α-phosphatidylserine competition assay compared to dilutions prepared in non-binding plates. Figure 3 As shown, these data suggest that wtMFG-E8 is partially lost during liquid handling and dilution steps when using polypropylene plates that have been optimized for low protein binding ( Figure 3A). These results suggest that the inherent stickiness of wtMFG-E8 presents challenges in the laboratory and most likely during drug manufacturing and production, where capture and polishing steps are required to produce high yields and very high purity drug substances. In contrast, the engineered protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO:44) was significantly less sticky than wtMFG-E8, and little difference was observed between dilutions performed in non-binding plates compared to those performed in polypropylene ( Figure 3 B). These data suggest that the insertion of solubilizing domains into the proteins of the present disclosure may improve their technological handling, thereby increasing step yields and, therefore, the overall yield during the manufacturing process.

[0368] Evaluation of fusion protein binding to L-α-phosphatidylserine Figure 4 The engineered MFG-E8-derived protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) bound to immobilized PS and to a lesser extent the phospholipid cardiolipin in a concentration-dependent manner ( Figure 4 A). Antibodies against the EGF-L domain of wtMFG-E8 were used to detect the binding of FP278 to immobilized L-α-phosphatidylserine or to cardiolipin (1,3-bis(sn-3'-phosphatidyl)-sn-glycerol). The binding strength of several recombinant fusion proteins to immobilized L-α-phosphatidylserine is shown in Figure 2. Figure 4 As shown in B. Human wtMFG-E8 and the fusion proteins FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) and FP260 (EGF-HSA-C1; SEQ ID NO: 34) effectively competed with 1 nM biotinylated mouse MFG-E8 for binding to immobilized L-α-phosphatidylserine in a concentration-dependent manner. Compared with human wtMFG-E8, the IC 50The values ​​represent highly similar L-α-phosphatidylserine binding strengths of the C1-C2 domains of the engineered protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44). Surprisingly, these data also indicate that the human C2 domain does not interact with L-α-phosphatidylserine or interacts only weakly with it, as shown by the results for FP270 (EGF-HSA-C2; SEQ ID NO: 36), which, along with FP250 (EGF-HSA; SEQ ID NO: 32), does not compete in this assay format. FP100 (EGF-C2-C2 protein (SEQ ID NO: 26)) was tested and did not compete in this assay format (not shown), leaving the C1 domain as the major PS binding moiety in human MFG-E8. This finding was unexpected, as a large body of literature suggests that the C2 domain of MFG-E8 is the primary domain responsible for PS binding (Andersen et al., (2000) Biochemistry, 39(20):6200-6; Shi & Gilbert (2003) Blood, 101:2628-2636; Shao et al., (2008) J Biol Chem., 283(11):7230-41). Taken together, these findings suggest that the C1 domain is the major overall PS binding domain of the MFG-E8 engineered protein and is important for PS binding-dependent functions. Thus, the C1 domain can be used to substitute into heterologous proteins to confer PS binding; however, the highest PS binding was demonstrated for fusion proteins containing either the C1-C2 or C1-C1 tandem domains (not shown).

[0369] 3.2αv integrin adhesion assay

[0370] The fusion protein was diluted in phosphate buffered saline (PBS) at pH 7.4 and 50 μL of a 24 nM solution was immobilized by adsorption (96-well plate, NuncMaxisorb) overnight (1.2 nM / well). The plate was then treated with PBS containing 3% fatty acid-free bovine serum albumin (BSA) for 1.5 hours at room temperature. Lymphoma cells expressing αvβ3 integrin (ATCC-TIB-48BW5147.G.1.4, ATCC, USA) were cultured in RPMI 1640 supplemented with GlutaMax, 25 mM HEPES, 10% FBS, Pen / Strep, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol. The cells were split one day before the adhesion experiment. Cells were labeled with 3 μg / mL 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Thermo Fisher Scientific Inc, USA) for 30 min. BW5147.G.1.4 cells were resuspended in adhesion buffer (TBS, 0.5% BSA, 1 mM MnCl 2 , pH 7.4) and 50,000 cells / well were allowed to adhere for 40 minutes at RT. Non-adherent cells were removed by repeated washing with adhesion buffer. TM The fluorescence of adherent cells was quantified using 2103 Multilabel Microplate Reader, PerkinElmer, USA. Data analysis was performed using MS Excel and GraphPad Prism software.

[0371] Cell adhesion to the immobilized fusion protein FP330 (EGF-HSA-C1-C2; SEQ ID NO: 42) was completely blocked by the αv integrin inhibitor cilengitide or 10 mM EDTA, indicating that integrin-dependent cell adhesion to the immobilized engineered protein ( Figure 5 A). A single point mutation (RGD>RGE) of the integrin binding motif RGD of the EGF-like domain (FP280; SEQ ID NO:38) resulted in complete abrogation of cell adhesion, indicating that a functional and accessible RGD binding motif in the fusion protein is essential for αv integrin-dependent adhesion ( Figure 5 B). The immobilized EGF-HSA protein FP250 (SEQ ID NO: 32) lacking the C1-C2 domain did not support or only barely supported the adhesion of BW5147.G.1.4 cells despite having an EGF-like domain ( Figure 5C). This finding suggests that under the experimental conditions tested, the RGD loop in the EGF-like domain fused to HSA may not be sufficiently accessible to cell surface integrins, possibly for steric reasons. Once C1, C2 or C1-C2 are fused to EGF-HSA at the C-terminal position, this interference is not evident. If expressed in CHO cells or HEK cells, the recombinant proteins of the present disclosure, such as FP330, promote αv-integrin-dependent cell adhesion similarly to wtMFG-E8 ( Figure 5 D).

[0372] Taken together, these data demonstrate that the fusion proteins of the present disclosure bind to cellular integrins and support integrin-dependent cell adhesion, and suggest that in proteins with HSA domain inserts, the C-terminal EGF-like domain can benefit from the C-terminally fused protein domain to support integrin binding.

[0373] 3.3 Human macrophage-neutrophil efferocytosis assay

[0374] By Ficoll gradient centrifugation ( -Paque PLUS, General Health Medical Group, Sweden) from buffy coat human peripheral blood mononuclear cells (PBMC), and then the monocytes were negatively selected using the stem cell isolation kit (stem cell 19059, Vancouver, Canada). Recombinant human M-CSF 40ng / mL (macrophage colony stimulating factor, R&D Systems, USA) was used to differentiate monocytes into "M0" macrophages in RPMI 1640 containing 25mM HEPES, 10% FBS, Pen / Strep, 1mM NaPyr, 50μM β-Merc for 5 days. One day before efferocytosis, macrophages were labeled with PKH26 using a red fluorescent dye linker kit (Sigma MINI26, USA). Cells were resuspended in RPMI 1640 containing 25 mM HEPES, 10% FBS, Pen / Strep, 1 mM NaPyr, 50 μM β-Merc and seeded at 40,000 cells / well in black 96-well plates (Corning, USA) and allowed to adhere for 20 hours.

[0375] Neutrophils: Sedimentation by dextran combined with Ficoll TM Human neutrophils were isolated from buffy coats by density gradient as follows: Buffy coat plasma was removed by centrifugation of the diluted buffy coats. The cell harvest was diluted with 1% dextran (from Leuconostoc sp., MW 450.000-650.000; Sigma, USA) and sedimented on ice for 20-30 minutes.

[0376] Leukocytes were collected from the supernatant and placed on Ficoll TM -Paque layer (General Health Medical Group Sweden). After centrifugation, the precipitate was collected and the remaining red blood cells were lysed using red blood cell (RBC) lysis buffer (BioConcept, Switzerland). Neutrophils were washed once in culture medium (RPMI 1640+GlutaMax containing 25mM HEPES, 10% FBS, Pen / Strep, 0.1mM NaPyr, 50uM b-Merc) and kept overnight at 15°C. Apoptosis / cell death was induced by treating neutrophils for 3 hours at 37°C with 1μg / mL Superfas Ligand (Enzo Life Sciences, Lausanne, Switzerland). Neutrophils were stained with Hoechst 33342 (Life Technologies, USA) for 25 minutes and stained with DRAQ5 (eBioscience, UK, 1:2000 dilution) at 37°C in the dark for 5 minutes.

[0377] Epilatory assay

[0378] M0 macrophages were incubated with the fusion protein for 30 minutes. Apoptosis-labeled neutrophils were added at a ratio of 1:4 M0 / neutrophils. The efferocytosis of apoptotic neutrophils by macrophages was visualized by the increase in DRAQ5 fluorescence intensity following neutrophil localization in the low pH lysosomal compartment of M0 macrophages.

[0379] Effervescence was quantified using the ImageXpress Micro XLS Wide Area High Content Analysis System (Molecular Devices, CA, USA). Macrophages were identified by PKH26 fluorescence. The efferocytosis index (EI, shown as %) was calculated as the ratio of macrophages containing at least one event of ingested apoptotic neutrophils (DRAQ5 high) to the total number of macrophages. Data analysis was performed using MS Excel and GraphPad Prism software.

[0380] Figure 6 The effect of the fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) on promoting the efferocytosis of dying neutrophils by human macrophages is shown in Figure 4. The fusion protein increased the internalization of dying human neutrophils labeled with pHrodo into macrophages, exceeding the high efferocytosis capacity of M0 macrophages (which was shown as basal levels). Figure 7In the present study, it was shown that the recombinant fusion protein FP278 can rescue the efferocytosis of dying neutrophils by human macrophages that were damaged by endotoxin (lipopolysaccharide). Figure 7 A shows efferocytosis of dying human neutrophils by impaired macrophages at 100 pg / ml of lipopolysaccharide (LPS) in three human donors. The left panel shows the response of a single donor, and the right panel shows the average efferocytosis (%) of the three donors. Figure 7 B shows that the fusion protein FP278 rescues the endotoxin (LPS)-impaired efferocytosis of dying neutrophils by human macrophages.

[0381] Figure 8 The fusion protein FP330 was shown to rescue human macrophages from efferocytosis of dying neutrophils impaired by S. aureus particles. Figure 8 A shows the effect of 100 nM of the fusion protein in promoting efferocytosis above the basal level (dashed line; left-hand portion of the graph), and the effect of 100 nM of the fusion protein in rescuing the impairment of efferocytosis caused by the addition of S. aureus (right-hand portion of the graph). Figure 8 B shows the increase of fusion protein FP278 (EC 50 8 nM) to rescue the impaired efferocytosis caused by the addition of S. aureus, as well as to promote efferocytosis once the basal level of efferocytosis was reached.

[0382] 3.4 Human endothelial cell-Jurkat efferocytosis assay

[0383] Cell culture

[0384] Human umbilical vein endothelial cells (HUVEC) were obtained from Lonza (Basel, Switzerland). Cells were cultured in flasks coated with gelatin (from bovine skin, final concentration 0.2% in PBS, dilution of 2% stock solution, Sigma, Germany). Cells were grown in medium 199 (Thermo Fisher Scientific, USA) supplemented with 10% FBS (GE Healthcare, UK), 1% Pen / Strep (Thermo Fisher Scientific, USA), 1% Glutamax (Thermo Fisher Scientific, USA) and 1 ng / mL recombinant fibroblast growth factor-basic (Peprotech, UK). Accutase was used. TM (Thermo Fisher Scientific, USA) to separate cells for harvesting or passaging.

[0385] Jurkat E6-1 cells were obtained from ATCC (American Type Culture Collection, USA) and grown in RPMI 1640 medium (Thermo Fisher Scientific, USA) supplemented with 10% FBS (GE Healthcare, UK), 1% Pen / Strep (Thermo Fisher Scientific, USA), 10 mM sodium pyruvate (Thermo Fisher Scientific, USA) and 10 mM HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid, Thermo Fisher Scientific, USA).

[0386] Recombinant human TRAIL (R&D Systems, USA) was used to induce apoptosis in Jurkat E6-1 cells. TM Green STP ester dye (Thermo Fisher Scientific, USA) was used to label apoptotic cells. Flow cytometry buffer was prepared with PBS (Thermo Fisher Scientific, USA) supplemented with 1% FBS (General Healthcare, UK), 0.05% w / v sodium azide (Merck, Germany) and 0.5 mM EDTA (ethylenediaminetetraacetic acid, Thermo Fisher Scientific, USA).

[0387] Epilatory assay

[0388] On day 1, use Accutase TM HUVECs (70%-90% confluence) were collected by separation for 5 minutes, washed with PBS and resuspended in cell culture medium. Cell number and viability were assessed using Guava EasyCyte flow cytometer (Merck, Germany) and Guava ViaCount reagent (Merck, Germany) according to the manufacturer's instructions. The required amount of cells was centrifuged at 300xg for 5 minutes at room temperature and resuspended in culture medium to a cell number of 6.6x10 4 150 μL / well of this cell suspension was added to a 96-well tissue culture plate (Corning TM HUVECs were cultured in 37°C / 5% CO 2 / 95% humidity incubator for another 16-20 hours.

[0389] Jurkat E6-1 cell number and viability / cell death status were assessed using a Guava EasyCyte flow cytometer (Merck, Germany) and GuavaViaCount reagent (Merck, Germany) according to the manufacturer's instructions. The required amount of cells was centrifuged at 300×g for 5 min at room temperature and lysed at 1×10 6The cells were resuspended at a density of 10 cells / mL in medium supplemented with recombinant human TRAIL at a final concentration of 50 ng / mL and incubated overnight at 37°C / 5% CO2 / 95% humidity to induce cell death.

[0390] On day 2, the medium was removed from the HUVECs by aspiration and 25 μL of fresh pre-warmed (37°C) medium was added, followed by 25 μL of fusion protein or control diluted in pre-warmed (37°C) medium. For dilutions, a non-binding surface (NBS) treated 96-well plate (Corning TM Before adding to dying Jurkat cells, the fusion protein was incubated at 37°C / 5% CO 2 / 95% humidity and interacted with HUVEC for 30 minutes.

[0391] The number of apoptotic / dying Jurkat E6-1 cells was counted using a Guava EasyCyte flow cytometer (Merck, Germany) and Guava ViaCount reagent (Merck, Germany). The required amount of apoptotic cells was centrifuged at 400×g for 5 min at room temperature and 5×10 6 The cells were resuspended at a density of 10 cells / mL in pHrodo supplemented with 5 μg / mL pHrodo TM Green STP ester dye was added to RPMI 1640 medium (without FBS) (staining medium). After staining at 37°C for 10 minutes, the remaining reactive pHrodo TM Green STP ester was inactivated with staining medium supplemented with 10% FBS at 37°C for 5 min. TM Wash the green labeled cells once and adjust the cell number to 3x10 in HUVEC medium. 6 cells / mL. 6 / Hole by pHrodo TM Green-labeled Jurkat cells were added to HUVEC and incubated at 37°C / 5% CO. 2 / 95% humidity for 5 hours. The medium was removed, HUVECs were washed once in PBS, and 40 μL / well of Accutase TM The solution was separated. 80 μL of ice-cold flow cytometry buffer was added to collect the cells, transferred to a 1.5 mL polypropylene 96-well block, washed with an excess of ice-cold flow cytometry buffer, and centrifuged at 400 x g (4 ° C) for 5 minutes. The supernatant was removed by suction, and the pellet was resuspended in 80 μL of ice-cold flow cytometry buffer and transferred to a 96-well V-bottom microtiter plate (BD Biosciences, USA). Then in a BD LSRFortessaTM The samples were measured on a flow cytometer (BD Biosciences, USA). TM Green fluorescence intensity was used as an indicator of lysosomal localization of engulfed Jurkat cells. TM pHrodo software for flow cytometry data analysis from single-gated HUVECs TM The median fluorescence intensity (MFI) value of the green signal was used as a readout. Data analysis was performed using MS Excel and GraphPadPrism software for EC 50 calculate.

[0392] The role of fusion proteins FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) and FP270 (EGF-HSA-C2; SEQ ID NO: 36) in promoting the efferocytosis of dying Jurkat cells by HUVEC endothelial cells Fig. 9 As shown. The fusion protein FP278 can effectively promote HUVEC internalization of pHrodo-labeled dying human Jurkat T cells. The results show that endothelial cells are armed with fusion proteins to become effective phagocytic cells of dying cells. Surprisingly, the efficacy of the fusion protein in this assay is clearly dependent on the presence of C1-C2 or C1-C1 tandem domains. For example, the fusion protein composed of EGF-HSA-C2 (FP270) is inactive in this experimental environment, as shown in Figure 2. Fig. 9 shown. Fig.10 Demonstrated our highly unexpected discovery that the location of the HSA domain in the engineered protein, i.e., the N- or C-terminal position (HSA-EGF-C1-C2 (FP220; SEQ ID NO: 30) or EGF-C1-C2-HSA (FP110; SEQ ID NO: 28, respectively)) confers efferocytosis blocking ability in a macrophage efferocytosis assay of the MFG-E8 HSA engineered protein. These data clearly demonstrate the importance of positioning the HSA domain between the integrin binding domain and the PS binding domain for effectively promoting efferocytosis of the fusion proteins of the present disclosure.

[0393] Fig.11 A comparison of the promotion of endothelial cell efferocytosis by various forms of fusion proteins including a combination of EGF domains, C1-C2 domains, HSA or Fc domains is shown. Fig.11A shows a comparison of fusion proteins comprising HSA, wherein HSA is located at the C-terminus or N-terminus or between the EGF-like domain and the C1-C2 domain; EGF-C1-C2-HSA (FP110; SEQ ID NO:28), HSA-EGF-C1-C2 (FP220; SEQ ID NO:30) and EGF-HSA-C1-C2-His tag (FP278; SEQ ID NO:44), respectively. Fig.11 B shows a comparison of fusion proteins containing an Fc domain (Fc is located at the C-terminus or between the EGF-like domain and the C1 domain). Two forms of the Fc portion are shown: the wild-type Fc (SEQ ID NO: 7) found in FP070 (EGF-Fc-C1-C2; SEQ ID NO: 17) and FP080 (EGF-C1-C2-Fc; SEQ ID NO: 22) and an Fc portion with KiH modifications S354C and T366W on one arm of the Fc (FP060; EGF-C1-C2-Fc [S354C, T366W]; SEQ ID NO: 14) EU numbering (Merchant et al. (1998) supra). Fig.11 C shows a comparison of the fusion protein FP090 (Fc-EGF-C1-C2; SEQ ID NO: 24) containing an Fc portion at the N-terminus for three batches of FP090 at three different concentrations (0.72, 7.2 and 72 nM), compared with a wtMFG-E8 control. The efferocytosis of dying Jurkat cells by HUVEC was only promoted by the engineered proteins with HSA or Fc portions inserted after the EGF-like domain. Fig.11 D shows that the insertion of the solubilization domain can generate a new biologically active fusion protein based on the endogenous bridging protein EDIL3 (a paralog of MFG-E8). Fig.11 As shown in D, HSA is inserted between the EGF-like domain and the C1-C2 domain of EDIL3, a paralog of MFG-E8. This EDIL3 construct (FP050 (EDIL3-based EGF-HSA-C1-C2; SEQ ID NO: 12) has only one of the three EGF-like domains found in wtEDIL3 (containing the RGD loop). In this construct, we unexpectedly found that the HSA domain insertion had similar tolerance for expression of novel recombinant engineered proteins with very high purity ( Figure 2 B). In addition, it was unexpectedly found that the recombinant engineered protein FP050 derived from EDIL3 promoted the efferocytosis of dying Jurkat cells by endothelial cells (HUVECS), demonstrating the core function of bridging proteins and exemplifying that the domains of bridging proteins can be used to design functional novel recombinant engineered proteins.

[0394] Example 4: Efferentiation of Prothrombotic Plasma Microparticles

[0395] 4.1 Human endothelial cell microparticle efferocytosis assay

[0396] Cell culture

[0397] HUVEC cells were obtained from Lonza (Basel, Switzerland). Cells were cultured in flasks coated with gelatin (from bovine skin, final concentration 0.2% in PBS, dilution of 2% stock solution, Sigma Aldrich / Merck, Germany). Cells were grown in medium 199 (Thermo Fisher Scientific, USA) supplemented with 10% FBS (GE Healthcare, UK), 1% Pen / Strep (Thermo Fisher Scientific, USA), 1% Glutamax (Thermo Fisher Scientific, USA) and 1 ng / mL recombinant fibroblast growth factor-basic (Peptech, UK). Accutase was used. TM (Thermo Fisher Scientific, USA) to separate cells for harvesting or passaging.

[0398] Platelet-derived microparticles were prepared according to the following procedure: Citrated venous blood (Coagulation 9NC Citrate Monovette, Sarstedt, Germany) was collected from healthy adult volunteers after written informed consent. Platelet-rich plasma (PRP) was prepared by centrifugation (200×g, 15 min, no brake, room temperature). Platelet-derived microparticles / fragments were generated by three cycles of quick freezing / freezing of PRP using liquid nitrogen and thawing at 37°C. Platelet fragments / microparticles were pelleted by centrifugation at 20'000×g for 15 min at RT. The pellet was resuspended in PBS, aliquots were prepared and stored at -80°C. Alexa Fluor TM The microparticle preparations were 85%-100% PS positive as determined by flow cytometry using 488 labeled murine MFG-E8 / lactamase (in-house at Novartis). The number of microparticles was determined using dedicated counting beads (BioCytex / Stago, France). Flow cytometry buffer was prepared with PBS (Thermo Fisher Scientific, USA) supplemented with 1% FBS (GE Healthcare, UK), 0.05% w / v sodium azide (Merck, Germany) and 0.5 mM EDTA (ethylenediaminetetraacetic acid, Thermo Fisher Scientific, USA).

[0399] 4.2 Cell burial assay

[0400] On day 1, use Accutase TM HUVEC cells (70%-90% confluence) were collected by separation for 5 minutes, washed with PBS and resuspended in cell culture medium. Cell number and viability were assessed using Guava EasyCyte flow cytometer (Merck, Germany) and Guava ViaCount reagent (Merck, Germany) according to the manufacturer's instructions. The required amount of cells was centrifuged at 300xg for 5 minutes at room temperature and resuspended in culture medium to a cell number of 6.6x10 4 150 μL / well of this cell suspension was added to a 96-well tissue culture plate (Corning TM HUVEC cells were cultured in an incubator at 37°C / 5% CO2 / 95% humidity for another 16-20 hours.

[0401] On day 2, the medium was removed from the HUVEC cells by aspiration and 25 μL of fresh pre-warmed (37°C) medium was added, followed by 25 μL of fusion protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) (three different concentrations: 0.3 nM, 3 nM or 30 nM) or control diluted in pre-warmed (37°C) medium. For dilutions, a non-binding surface (NBS) treated 96-well plate (Corning TM Before adding platelet-derived microparticles, the test protein was incubated at 37°C / 5% CO 2 / 95% humidity and interacted with HUVEC cells for 30 minutes.

[0402] The required amount of microparticles was centrifuged at 20'000 x g for 15 min at 4°C and precipitated at 2 x 10 8 The particles were resuspended at a density of 1000 particles / mL in pHrodo 5% ethanol supplemented with a final concentration of 5 μg / mL. TM Green STP ester dye was added to RPMI 1640 medium (without FBS) (staining medium). After staining at 37°C for 10 minutes, the remaining reactive pHrodo TM Green STP ester was inactivated with staining medium supplemented with 10% FBS at 37°C for 5 min. TM Green labeled microparticles were washed once by centrifugation at 20'000xg for 15 min at 4°C and the number was adjusted to 1x10 in HUVEC cell culture medium. 8 particles / mL. 6 Particles / well pHrodo TM Green labeled microparticles were added to HUVEC cells and incubated at 37°C / 5% CO. 2 / 95% humidity for 5 hours. The culture medium was removed, HUVEC cells were washed once in PBS, and 40 μL / well of Accutase TM The solution was separated. 80 μL of ice-cold flow cytometry buffer was added to collect the cells, transferred to a 1.5 mL polypropylene 96-well block, washed with excess ice-cold flow cytometry buffer, and centrifuged at 400×g (4° C.) for 5 minutes. The supernatant was removed by aspiration, and the pellet was resuspended in 80 μL of ice-cold flow cytometry buffer and transferred to a 96-well V-bottom microtiter plate (BD Biosciences, USA). In a BD LSRFortessa TM The samples were measured on a flow cytometer (BD Biosciences, USA). TM Green fluorescence intensity was used as an indicator of lysosomal localization of engulfed microparticles. TM pHrodo software for flow cytometry data analysis from single-gated HUVEC cells TM The median fluorescence intensity value (MFI) of the green signal was used as a readout. Data analysis was performed using MS Excel and GraphPad Prism software for EC 50 The fusion protein FP278 promoted the efferocytosis of platelet-derived microparticles by endothelial cells in a concentration-dependent manner. Fig.12 The enhancement of uptake was concentration-dependent and was also observed in other endothelial cell types (not shown).

[0403] Example 5: Technical characteristics of MFG-E8-HSA fusion protein

[0404] 5.1 Surface plasmon resonance (SPR) binding analysis of fusion protein FP330 and FcRn

[0405] A direct binding assay was performed to characterize the binding of the fusion protein FP330 (EGF-HSA-C1-C2; SEQ ID NO: 42) to FcRn. The kinetic binding affinity constant (KD) was measured on the captured protein using recombinant human FcRn as the analyte. The kinetic binding affinity constant (KD) was measured at room temperature and at pH 5.8 and 7.4, respectively. Measurements were performed on a T200 (GE Healthcare, Glattbrugg, Switzerland). For affinity measurements, proteins were diluted in 10 mM NaP, 150 mM NaCl, 0.05% Tween 20 (pH 5.8) and immobilized on a flow cell of a CM5 research grade sensor chip (GE Healthcare, reference BR-1000-14) using standard procedures according to the manufacturer's recommendations (GE Healthcare). For reference, one flow cell was immobilized as a blank. Binding data were obtained by subsequent injection of analyte dilution series on the reference and measurement flow cells. Zero concentration samples (operating buffer only) were included to allow dual referencing during data evaluation. For data evaluation, dual referenced sensorgrams were used and the dissociation constant (KD) was analyzed.

[0406] Fusion protein FP330 binds to FcRn with an affinity of 1380 nM at pH 5.8, while no binding is observed at pH 7.4 (see Table 5 above). These results are well consistent with wild-type HSA (1000-2000 nM, pH 5.8, data not shown).

[0407] 5.2 Differential Scanning Calorimetry (DSC) of MFG-E8 and its variants

[0408] The thermal stability of the engineered MFG-E8 protein variant FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) was measured using differential scanning calorimetry. The measurements were performed on a differential scanning microcalorimeter (Nano DSC, TA instruments). The cell volume was 0.5 ml and the heating rate was 1 °C / min. The protein was used at a concentration of 1 mg / ml in PBS (pH 7.4). The molar heat capacity of the protein was estimated by comparison with duplicate samples containing the same buffer (without the protein). Partial molar heat capacity and melting curves were analyzed using standard procedures. The thermogram was baseline corrected and concentration normalized. Two melting events were observed, the first Tm at 50 °C and the second Tm at 64 °C.

[0409] 5.3 Aggregation propensity and solubility measurements of MFG-E8 variants

[0410] First, the aggregation tendency of the MFG-E8 variant protein FP278 (EGF-HSA-C1-C2-His tag; SEQ ID NO: 44) was measured by dynamic light scattering (DLS, Wyatt). Dynamic light scattering was used to measure the translational diffusion coefficient of FP278 in solution by quantifying the dynamic fluctuations in the scattered light. The size distribution of the unfractionated protein variants provided an estimate of the polydispersity as well as the hydrodynamic radius, which was measured at a concentration of 1 mg / ml. The DynaPro TM The hydrodynamic radius of the fusion protein FP278 was determined by a plate reader (Wyatt Technology Europe GmbH, Dernbach, Germany) in combination with the software DYNAMICS (version 7.1.0.25, Wyatt). 50 μL of undiluted and filtered (0.22 μm PVDF-filter ( Syringe driven filter unit, Millipore, Billerica, USA) protein solution. Higher molecular weight aggregates of the protein sample could not be identified. The hydrodynamic radius of the protein was approximately 5-6 nm, indicating the presence of monomeric protein in the solution.

[0411] Secondly, the fusion protein FP278 was subjected to concentration-dependent hydrodynamic radius measurements to estimate the solubility of the protein. Protein concentrations up to 22 mg / ml were applied. The hydrodynamic radius was determined as described above. After the increase in the concentration of the fusion protein FP278, no increase in radius (5-7 nm) was observed, while the dynamic light scattering measurement of wtMFG-E8 (SEQ ID NO: 1) failed due to high aggregation at a concentration of about 0.2 mg / ml.

[0412] Example 6: Optimization of MFG-E8 fusion protein

[0413] Mass spectrometry (MS) was used to study the fusion protein FP330 (EGF-HSA-C1-C2) to generate a set of fusion proteins based on the variant MFG-E8 that were optimized to improve expression and yield. A set of variant proteins with different sizes and structures of linkers (e.g., linkers containing GS between the EGF and HSA domains and / or linkers containing multiples of GS or G4S between the HSA and C1 domains) were generated. In addition, amino acid modifications including deletions or substitutions were included in some of the variants (shown as HSA* in Table 7). The set of variant fusion proteins is summarized in Table 7 below.

[0414] Table 7: Summary of variant fusion proteins

[0415]

[0416] 1 The positions of amino acid modifications are numbered according to SEQ ID NO: 42 (FP330).

[0417] Example 7: Variant MFG-E8 Fusion Protein; Expression and Purification

[0418] The method of producing fusion proteins in HEK cell lines is described in Example 2. For expression in a proprietary CHO cell line, nucleic acids encoding MFG-E8 variants were synthesized at Geneart (Life Technologies) and cloned into mammalian expression vectors using restriction enzyme ligation-based cloning techniques. The resulting plasmid was transfected into CHO-S cells (Thermo). In short, in order to transiently express the fusion protein, the expression vector was transfected into suspension-adapted CHO-S cells using Expifectamine CHO transfection agent (Thermo). Typically, 400 ml of cells suspended at a density of 6 Mio cells / ml were transfected with DNA containing 400 μg of the expression vector encoding the engineered protein. The recombinant expression vector was then introduced into the host cell for further secretion for seven days in the culture medium (ExpiCHO expression medium, supplemented with ExpiCHO feed and enhancer (Thermo)).

[0419] As can be seen from the expression data shown in Table 8, the expression of variant fusion proteins FP068 (SEQ ID NO: 46) and FP776 (SEQ ID NO: 48) was approximately two times higher than that of fusion protein FP330 (SEQ ID NO: 42).

[0420] Table 8: Expression of fusion proteins in HEK and CHO* cell lines

[0421]

[0422] *Indicates fusion proteins produced in CHO cell lines

[0423] Other therapeutic fusion proteins were obtained according to the method described in Example 1. For example, the expression levels (mg / l) obtained after the complete purification process (capture and polish) were 4.3 for Seq ID 80 and 8.4 for Seq ID 82.

[0424] Example 8: Characterization of variant fusion proteins

[0425] As described in Example 3, the effect of the variant fusion proteins on efferocytosis was determined by performing an efferocytosis assay.

[0426] In the first assay, the effect of the variant fusion proteins in the human macrophage-neutrophil efferocytosis assay was determined according to the method described in Section 3.3 above. M0 macrophages were incubated with the fusion protein FP330 (EGF-HSA-C1-C2; SEQ ID No: 42) or variants FP278 (EGF-HSA-C1-C2-His tag; SEQ ID No: 44) or FP776 (EGF-HSA-C1-C2; SEQ ID No: 48) for 30 minutes. Fig.13 As shown, fusion proteins FP330, FP278 and FP776 can rescue the efferocytosis of dying neutrophils by human macrophages that is impaired by endotoxin (lipopolysaccharide (LPS)). 50 =1.6nM; Fig.13 A) FP278 (EC 50 =1.78nM; Fig.13 B) and FP776 (EC 50 =0.5nM; Fig.13 C) leads to the rescue of impaired efferocytosis caused by the addition of LPS, and the tagging even promotes efferocytosis after reaching basal levels.

[0427] Fusion proteins FP330, FP278 and FP776 were further characterized in a human endothelial (HUVEC) cell-Jurkat cell efferocytosis assay according to the methods described in Section 3.4 above. The effects of fusion proteins FP330, FP278 and FP776 in promoting HUVEC efferocytosis of dying Jurkat cells are shown in Fig.14 By increasing FP330 (EC 50 =3.4nM; Fig.14 A) FP278 (EC 50 =2.4nM; Fig.14 B) and FP776 (EC 50 =3nM; Fig.14 C) concentrations to strongly promote HUVEC internalization of pHrodo-labeled dying human Jurkat T cells. These results indicate that endothelial cells are armed with the fusion protein to become efficient phagocytic cells of dying cells.

[0428] Example 9: Protection of mice from AKI and AKI-triggered acute organ responses

[0429] 9.1 Acute Kidney Injury Model

[0430] Female C57BL / 6 mice (18-22 g) were purchased from Charles River, France, and placed in a temperature-controlled apparatus in a cage protected by a top filter (12-hour light / dark cycle). Animals were treated in strict accordance with Swiss federal law and NIH principles of laboratory animal care. The tested therapeutic fusion protein was administered intraperitoneally (ip) or intravenously (iv) two hours before surgery. Buprenorphine (Indivior Schweiz AG) was injected subcutaneously (sc) at a dose of 0.1 mg / kg 60 to 30 minutes before surgery. Inhalation anesthesia using isoflurane was induced in an anesthesia chamber (3.5-5 Vol.%, carrier gas: oxygen) for 5 minutes before surgery. During surgery, animals were maintained under anesthesia with 1-2 Vol% isoflurane / oxygen by mask, with a gas flow rate of 0.8-1.2 l / min. The abdominal skin was shaved and disinfected with Betaseptic (Moody Drug Company, France). The animals were placed on a thermostatic blanket (Rothacher-Switzerland) with a thermostatic monitoring system (PhysiTemp, US-Physitemp Instruments LLC, USA) and covered with sterile gauze. Throughout the operation, body temperature was monitored by a rectal probe (Physitemp Instruments, USA) and controlled to be 36.5°C-37.5°C. All animals, including sham controls, underwent unilateral nephrectomy of the right kidney: after a midline incision / laparotomy, the abdominal contents were retracted to the left to expose the right kidney. The right ureter and renal blood vessels were disconnected and ligated, and then the right kidney was removed. For animals with AKI, the abdominal contents were placed on the right side on sterile gauze, and the left renal artery and vein were dissected to clamp for inducing ischemia. The renal pedicle was clamped using a microaneurysm clip (B Braun, Switzerland) (one clip was used to clamp the artery and vein together) to block blood flow to the kidney and induce renal ischemia. The color change of the kidney from red to dark purple confirmed successful ischemia, which occurred within seconds. After induction of ischemia (35-38 minutes), the microaneurysm clip was removed. Before wound closure, warm sterile saline (about 2 ml, 37 ° C) was used to rinse the abdominal contents to hydrate the tissue. After washing, 1 ml of sterile saline was added intraperitoneally as a rehydration. When reperfusion began, the wound was closed in two layers (muscle and skin, respectively). The animals were then kept under a red warm lamp until fully recovered. Buprenorphine was administered again 1 hour and 4 hours after surgery at a dose of 0.1 mg / kg, and was also included in drinking water (9.091 μg / mL). After 24 hours, the animals were euthanized for analysis.

[0431] 9.2 Administration of Therapeutic Fusion Proteins

[0432] Therapeutic fusion proteins FP330 (EGF-HSA-C1-C2; SEQ ID No: 42), FP278 (EGF-HSA-C1-C2-His tag; SEQ ID No: 44) and FP776 (EGF-HSA-C1-C2; SEQ ID No: 48) were tested in the AKI model as described above at the doses listed in Table 9 below. For studies to detect serum markers and qPCR marker expression, fusion protein FP278 was administered 2 hours before surgery. FP330 and FP776 were administered intravenously. 30 minutes before the onset of ischemia-reperfusion injury. For studies to measure contrast agent uptake by magnetic resonance imaging, fusion protein FP776 was administered prophylactically at a dose of 1.26 mg / kg 30 minutes before AKI induction, or therapeutically intravenously at 2 mg / kg 5 hours after ischemia-reperfusion injury induction.

[0433] Table 9: Administration of therapeutic fusion proteins

[0434]

[0435]

[0436] 9.3 Readout / AKI Protection Analysis:

[0437] Serum markers:

[0438] Serum samples were taken 24 hours after induction of ischemia-reperfusion and analyzed for serum creatinine and blood urea nitrogen (BUN) content using a Hitachi M40 clinical analyzer according to the manufacturer's instructions (Axonlab, Switzerland).

[0439] qPCR marker expression in organs:

[0440] AKI is induced 24 hours after harvesting organs (kidney, liver, lung and heart), and cut into 1 cm fragments, and stored overnight in RNA late buffer (RNA Later buffer) (Thermo Fisher Scientific, the United States) at 4 ° C. Organ fragments are transferred to RLT buffer (RNeasy micro kit, Qiagen, DE) containing 134mM β-mercaptoethanol (Merck, Germany) in Lysing Matrix D (Lysing Matrix D) tube (MP Biomedicals, France), and homogenized using FastPrep-24 instrument (MP Biomedicals). Subsequently, the cardiac fibrous tissue is digested with proteinase K (RNeasy micro kit), while in microcentrifuge (Eppendorf, Germany), kidney, liver and lung lysates are directly centrifuged at full speed for 3 minutes. The supernatant is transferred to QIAshredder spin column (Qiagen, Germany) and centrifuged for 2 minutes. RNA extraction was performed on the flow-through liquid according to the RNeasy micro kit manual (including DNA enzyme digestion). RNA concentration was measured using Nano Drop 1000 equipment (Thermo Fisher Scientific). Using SimpliAmp Thermocycler (Applied Biosystems, USA), according to the high-capacity cDNA reverse transcription kit manual (Thermo Fisher Scientific), 2 μg RNA of each sample was reverse transcribed. cDNA was combined with nuclease-free water (Thermo Fisher Scientific), TaqMan probe (TaqMan gene expression assay (FAM), Thermo Fisher Scientific) and TaqMan gene expression premix (Thermo Fisher Scientific) in 384-well microplates (MicroAmp optical 384-well reaction plates, Thermo Fisher Scientific). qPCR was performed on ViiA 7 real-time PCR systems (Applied Biosystems, USA). The settings were 1: 2 min, 50 °C; 2: 10 min, 95 °C; 3: 15 s, 95 °C; 4: 1 min, 60 °C. Repeat steps 3 and 4 for 45 cycles. Data analysis was performed using ViiA 7 software, and qPCR data analysis software was performed using MS Excel and GraphPad Prism software.

[0441] Hepatic uptake of contrast agents measured by magnetic resonance imaging (MRI)

[0442] The method for performing MRI was adapted from the publication of Egger et al. (Egger et al., (2015) J Magn Reson Imaging, 41:829-840). The experiments were performed on a 7-T Bruker Biospec MRI system (BrukerBiospin, Ettlingen, Germany). During MRI signal acquisition, mice were placed supine in a Plexiglas holder. Body temperature was maintained at 37°C ± 1°C with a heating pad. After a short induction period, the mice were treated with O administered via a nose cone. 2 / N 2 Anesthesia was maintained with approximately 1.4% isoflurane in an O (1:2) mixture. All measurements were performed on spontaneously breathing animals; neither cardiac nor respiratory triggering was applied.

[0443] After placing the mouse in the scanner, rapid reconnaissance images can be acquired for localization purposes. Perfusion analysis was performed using intravascular administration of drugs in Guerbet (Guerbet, France). The bolus was injected intravenously for 1.2s into animals with AKI. The first bolus was applied in 1.2s, and echo planar images were collected sequentially with a resolution of 400ms / image. After collecting 25 baseline images, the second bolus was applied in 1.2s, and 575 images were collected after the bolus, thereby obtaining a total of 600 images in 4 minutes. Superparamagnetic contrast agents cause local changes in sensitivity, which causes signal attenuation to be proportional to renal perfusion. For a series of images, the region of interest (ROI) located in the cortex / outer medulla outer striae was evaluated for signal intensity. The position, shape and size of the ROI were carefully selected to ensure that although respiration causes kidney movement, they cover approximately the same area. The average signal intensity of the image before injection provides a baseline intensity (S(0)). The perfusion index is determined according to the mean value of the following ratio (Rosen et al., (1990) Magn Reson Med. [magnetic resonance in medicine], 14: 249-265):

[0444] -ln[S(t) / S(0)]~TE.V.cT(t)

[0445] where TE is the echo time, V is the blood volume, and cT is the concentration of the contrast agent.

[0446] The average diameter of the SPIO nanoparticles used in the study was about 150 nm and was taken up by Kupffer cells in the liver. Therefore, in addition to renal perfusion, MRI can also monitor the uptake of nanoparticles in the liver by detecting contrast changes assessed in ROIs located in the liver.

[0447] 9.4 Results

[0448] like Fig.15 As shown, the fusion proteins FP330 (EGF-HSA-C1-C2; SEQ ID No: 42), FP278 (EGF-HSA-C1-C2-His tag; SEQ ID No: 44) and FP776 (EGF-HSA-C1-C2; SEQ ID No: 48) protected renal function in this acute kidney injury (AKI) model when administered intraperitoneally (FP278) or intravenously (FP330 and FP776). This protective effect was reflected by the blockade of serum creatinine elevation (sCr). Fig.15 A shows that fusion protein FP278 significantly reduced serum creatinine levels at both doses tested compared to vehicle-treated animals (p<0.0001) and was as effective as murine MFG-E8. Fig.15 As shown in B, fusion protein FP330 protected renal function in a dose-dependent manner, and fusion protein FP776 ( Fig.15 C) The same is true, where serum creatinine levels are also blocked in a dose-dependent manner.

[0449] Impaired renal function was also reflected in the blood urea nitrogen (BUN) levels of the tested mice, and the effect of the fusion protein FP278 on BUN levels was shown in Figure 2 Fig.16 shown.

[0450] In short, if Fig.15 and 16 As shown, the fusion proteins FP278, FP330 and FP776 potently prevented the elevation of these markers used in the clinical diagnosis of renal failure. The observed efficacy was confirmed by histology (not shown).

[0451] In addition, if Fig.17 As shown, a single dose of fusion protein FP278 protects distant organs from acute phase responses caused by AKI. AKI induces excessive mRNA responses, which can be measured by qPCR in lysates of highly perfused organs (e.g., spleen, lung, liver, heart, and brain) at a distance. Typical mRNA induces selective damage (NGAL, KIM-1), induction of chemokines (not shown), or induction of acute phase response proteins (e.g., serum amyloid A (SAA)). Fig.17A and 17B illustrate this AKI-induced response (serum amyloid A (SAA)) in mouse heart and lung, which was robustly blocked and restored to sham levels after a single injection of the fusion protein.

[0452] Liver response to SPIO contrast agent over time Intake of Fig.18 As shown. Animals with AKI showed a significant reduction in the uptake of contrast agents by the liver compared to sham-operated animals (target = Kupffer cells). FP776 treatment (administered prophylactically at 1.26 mg / kg at -30 minutes before AKI induction, or therapeutically at 2 mg / kg at +5 hours after ischemia-reperfusion injury induction) protected AKI mice from loss of contrast agent accumulation in the liver. These results indicate that in this mouse model, AKI causes significant impairment of endogenous Kupffer cell-mediated microparticle clearance, and AKI causes microvascular disturbances, which affect the accumulation of iron particle contrast agents in the liver. Treatment with the fusion protein FP776 prevents loss of clearance and microvascular disturbances compared to sham-operated animals, and can even enhance the uptake of contrast agents at both tested doses.

[0453] Example 10: Characterization of MFG-E8-HSA Engineered Protein

[0454] 10.2αv Integrin Adhesion Assay

[0455] The fusion protein was diluted in phosphate buffered saline (PBS) at pH 7.4 and 50 μL of the indicated concentration was fixed by adsorption (96-well plate, NuncMaxisorb) overnight. The plate was then treated with PBS containing 3% fatty acid-free bovine serum albumin (BSA) for 1.5 hours at room temperature. Lymphoma cells expressing αvβ3 integrin (ATCC-TIB-48BW5147.G.1.4, ATCC, USA) were cultured in RPMI 1640 supplemented with GlutaMax, 25 mM HEPES, 10% FBS, Pen / Strep, 1 mM sodium pyruvate, 50 μM β-mercaptoethanol. Cells were labeled with 3 μg / mL 2',7'-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Thermo Fisher Scientific, USA) for 30 minutes. BW5147.G.1.4 cells were resuspended in adhesion buffer (TBS, 0.5% BSA, 1 mM MnCl2, pH 7.4) and 50,000 cells / well were allowed to adhere for 40 minutes at RT. Non-adherent cells were removed by manual washing with adhesion buffer. Envision TMThe fluorescence of adherent cells was quantified using 2103 Multi-label Microplate Reader (PerkinElmer, USA). Data analysis was performed using MS Excel and GraphPad Prism software.

[0456] Adhesion of BW5147.G.1.4 cells to immobilized EGF-like domain containing fusion proteins This finding suggests that under the experimental conditions tested, the RGD loop in the EGF-like domain fused to HSA of MFG-E8 or EDIL3 / DEL-1 based fusion proteins is accessible and allows interaction with cellular av integrins.

[0457] Taken together, these data demonstrate that the fusion proteins of the disclosure bind to cellular integrins, support integrin-dependent cell adhesion, and demonstrate retention of function in proteins with an HSA domain insert.

[0458] 10.3 Human macrophage-neutrophil efferocytosis assay

[0459] By Ficoll gradient centrifugation ( -Paque PLUS, General Health Medical Group, Sweden) from buffy coat human peripheral blood mononuclear cells (PBMC), and then the monocytes were negatively selected using the stem cell isolation kit (stem cell 19059, Vancouver, Canada). Recombinant human M-CSF 40ng / mL (macrophage colony stimulating factor, R&D Systems, USA) was used to differentiate monocytes into "M0" macrophages in RPMI 1640 containing 25mM HEPES, 10% FBS, Pen / Strep, 1mM NaPyr, 50μM β-Merc for 5 days. One day before efferocytosis, macrophages were labeled with PKH26 using a red fluorescent dye linker kit (Sigma MINI26, USA). Cells were resuspended in RPMI 1640 containing 25 mM HEPES, 10% FBS, Pen / Strep, 1 mM NaPyr, 50 μM β-Merc and seeded at 40,000 cells / well in black 96-well plates (Corning, USA) and allowed to adhere for 20 hours.

[0460] Neutrophils: Sedimentation by dextran combined with Ficoll TM Human neutrophils were isolated from buffy coats by density gradient as follows: Buffy coat plasma was removed by centrifugation of the diluted buffy coats. The cell harvest was diluted with 1% dextran (from Leuconostoc sp., MW 450.000-650.000; Sigma, USA) and sedimented on ice for 20-30 minutes.

[0461] Leukocytes were collected from the supernatant and placed on Ficoll TM -Paque layer (GE Healthcare Sweden). After centrifugation, the precipitate was collected and the remaining red blood cells were lysed using red blood cell (RBC) lysis buffer (BioConcept, Switzerland). Neutrophils were washed once in culture medium (RPMI 1640+GlutaMax containing 25mM HEPES, 10% FBS, Pen / Strep, 0.1mM NaPyr, 50uM b-Merc) and kept overnight at 15°C. Apoptosis / cell death was induced by treating neutrophils with 1μg / mL Superfas Ligand (Enzo Life Sciences, Lausanne, Switzerland) for 3 hours at 37°C. Neutrophils were stained with Hoechst 33342 (Life Technologies, USA) for 25 minutes and stained with DRAQ5 (e Biosciences, UK, 1:2000 dilution) at 37°C in the dark for 5 minutes.

[0462] Epilatory assay

[0463] M0 macrophages were incubated with the fusion protein for 30 minutes. Apoptosis-labeled neutrophils were added at a ratio of 1:4 M0 / neutrophils. The efferocytosis of apoptotic neutrophils by macrophages was visualized by the increase in DRAQ5 fluorescence intensity following neutrophil localization in the low pH lysosomal compartment of M0 macrophages.

[0464] Efferocytosis was quantified using the ImageXpress Micro XLS Wide Area High Content Analysis System (Molecular Devices, CA, USA). Macrophages were identified by PKH26 fluorescence. The efferocytosis index (EI, shown as %) was calculated as the ratio of macrophages containing at least one event of ingested apoptotic neutrophils (DRAQ5 high) to the total number of macrophages. Data analysis was performed using MS Excel and GraphPad Prism software. Fig.13 The effects of fusion proteins FP114 and FP133 (MFG-E8 derived EGF-HSA-C1 SEQ ID NO:xxx) in rescuing and promoting efferocytosis of dying neutrophils by LPS treated human macrophages are shown in D. The fusion proteins increased the internalization of dying human neutrophils labeled with pHrodo into macrophages, exceeding the already high efferocytosis capacity of M0 macrophages. Fig.13In E, it is shown that the recombinant fusion protein FP147 (EDIL / DEL-1 derived EGF_EGF_EGF_HSA_C1) can rescue endotoxin (lipopolysaccharide) impaired efferocytosis of dying neutrophils by human macrophages. Overall, the data show the unexpected finding that C2-truncated MFGE8 or EDIL3 / DEL-1 derived fusion proteins can promote efferocytosis in vitro with low nM efficacy.

[0465] Example 11: Protection of mice from AKI

[0466] 11.1 Acute Kidney Injury Model

[0467] Female C57BL / 6 mice (18-22 g) were purchased from Charles River, France, and placed in a temperature-controlled device in a cage protected by a top filter (12-hour light / dark cycle). Animals were treated in strict accordance with Swiss federal law and NIH principles of laboratory animal care. The tested therapeutic fusion protein was administered intraperitoneally (ip) or intravenously (iv) two hours before surgery. Buprenorphine (Indivior Schweiz AG) was injected subcutaneously (sc) at a dose of 0.1 mg / kg 60 to 30 minutes before surgery. Inhalation anesthesia using isoflurane was induced in an anesthesia chamber (3.5-5 Vol.%, carrier gas: oxygen) for 5 minutes before surgery. During surgery, animals were maintained under anesthesia with 1-2 Vol% isoflurane / oxygen by mask, with a gas flow rate of 0.8-1.2 l / min. The abdominal skin was shaved and disinfected with Betaseptic (Mundipharma, France). The animals were placed on a thermostatic blanket (Rottacher-Switzerland) with a thermostatic monitoring system (PhysiTemp, Inc., Physitemp Instruments, USA, USA) and covered with sterile gauze. Throughout the operation, body temperature was monitored by a rectal probe (Physitemp Instruments, USA) and controlled to be 36.5°C-37.5°C. All animals, including sham controls, underwent unilateral nephrectomy of the right kidney: after a midline incision / laparotomy, the abdominal contents were retracted to the left to expose the right kidney. The right ureter and renal blood vessels were disconnected and ligated, and then the right kidney was removed. For animals with AKI, the abdominal contents were placed on the right side on sterile gauze, and the left renal artery and vein were dissected to be clamped for inducing ischemia. The renal pedicle was clamped using a microaneurysm clip (B Braun, Switzerland) (using a clip to clamp the artery and vein together) to block blood flow to the kidney and induce renal ischemia. The color change of the kidney from red to dark purple confirmed successful ischemia, which occurred within a few seconds. After induction of ischemia (35-38 minutes), the microaneurysm clip is removed. Before wound closure, warm sterile saline (about 2ml, 37°C) is used to rinse the abdominal contents to hydrate the tissue. After washing, 1ml sterile saline is added intraperitoneally as a rehydration. When reperfusion begins, the wound is closed in two layers (muscle and skin, respectively). The animals are then kept under a red warm lamp until fully recovered. Buprenorphine is administered again 1 hour and 4 hours after surgery at a dose of 0.1mg / kg, and is also included in drinking water (9.091μg / mL). After 24 hours, the animals are euthanized for analysis. Therapeutic fusion protein FP135 (EGF-HSA-C1; SEQ ID No: x) was tested 30 minutes before the onset of ischemia-reperfusion injury in the AKI model with 1.5mg / kg iv.Serum samples were taken 24 hours after induction of ischemia-reperfusion and analyzed for serum creatinine and blood urea nitrogen (BUN) content using a Hitachi M40 clinical analyzer according to the manufacturer's instructions (Axonlab, Switzerland).

[0468] Example 12: EGF_HSA_C1 protects against hepatic fibrosis in a liver fibrosis model (CCL4 model)

[0469] Liver fibrosis is a wound healing response to various insults. If advanced, it may lead to cirrhosis and subsequently hepatocellular carcinoma (HCC). In industrialized countries, common causes of liver fibrosis are alcohol abuse, viral hepatitis infection, and metabolic syndrome due to obesity, insulin resistance, and diabetes.

[0470] Prolonged insult leads to inflammation and deposition of extracellular matrix (ECM) proteins by myofibroblast-like cells, essentially activated hepatic stellate cells (HSCs). These cells produce alpha smooth muscle actin (αSMA) and deposit collagen types I and III, and produce matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). As the disease becomes chronic, the composition of the ECM shifts from collagen types IV and VI, glycoproteins, and proteoglycans to collagen types I and III and fibronectin.

[0471] If the injury is not severe, the liver is able to regenerate, whereby adjacent mature hepatocytes replace apoptotic or necrotic cells. Regression of fibrosis occurs when activated HSCs undergo apoptosis or revert to a more quiescent phenotype.

[0472] There are several in vivo models that can be used to simulate various aspects of the disease. Liver fibrosis models need to reflect the various pathological and molecular features of human disease and be easy to establish and have good reproducibility. Chemically induced fibrosis models are the closest to these ideal features, one of which is carbon tetrachloride (CCl) in rodents. 4 ) Liver fibrosis model. After repeated intraperitoneal injections of this hepatotoxin, liver fibrosis develops, showing good similarity to human liver fibrosis. In addition, withdrawal of the invasive lesion leads to regression of fibrosis, so this model is reversible.

[0473] In the first phase, CYP2E1 enzyme metabolizes CCl 4 , generating trichloromethyl radicals that contribute to an acute phase reaction characterized by the destruction of lipid membranes and internal organelles of hepatocytes, ultimately leading to necrosis. Acute CCl4-mediated liver fibrosis is then characterized by the activation of Kupffer cells and the induction of an inflammatory response, leading to the secretion of cytokines, chemokines, and other pro-inflammatory factors. This, in turn, attracts and activates monocytes, neutrophils, and lymphocytes, which in turn contribute to liver necrosis, followed by a strong regenerative response, leading to the first application of CCl4 About 48 hours after CCl4, hepatocytes and non-parenchymal hepatocytes proliferate in large quantities. Histological fibrosis and scar fibers appear in the second stage of the disease after 2 to 3 weeks. After 4 to 6 weeks of CCl4 damage, the third stage (with extensive fibrosis and a large amount of liver fat accumulation and elevated serum triglycerides and AST levels) can be observed. Usually after the withdrawal of CCl4, the liver is completely healed. 4 CCl can be observed within weeks after 4 Complete regression of induced liver fibrosis in mice. Drugs with the property of accelerating regression of fibrosis are particularly relevant to patients with established disease. For example, patients with NASH (nonalcoholic steatohepatitis) chronic kidney disease or scleroderma who already have established fibrosis demonstrate that regression of fibrosis may become a primary clinical endpoint, not only to halt the disease but also to restore organ function. (Yanguas et al. 2016. Experimental models of liver fibrosis. Arch Toxicol. 2016;90:1025-1048. doi:10.1007 / s00204-015-1543-4.)

[0474] CCL4 liver fibrosis model:

[0475] Disease Induction:

[0476] Male BALB / c mice aged 8-12 weeks were injected intraperitoneally with CCl 3 times per week for 6 weeks. 4 , dosed at 500 μl / kg (freshly diluted in olive oil). Netherlands). CCl 4 A total of 6 weeks were used to induce liver fibrosis. Treatment with EGF_HSA_C1 (FP135) was started after 4, 5 or 6 weeks of CCL4 treatment. EGF_HSA_C1 (FP135) was administered intraperitoneally at 0.8 mg / kg 3 times per week until the termination of the experiment (3 days after stopping CCL4).

[0477] reading:

[0478] Liver enzymes such as ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were measured in serum samples obtained at the time of CCL4 cessation (day 0) and 3 days after the termination of the experiment to assess liver damage. ALT and AST were analyzed using a Hitachi M40 clinical analyzer according to the manufacturer's instructions (Axonlab, Switzerland).

[0479] To quantify the amount of collagen in the liver of the animals, hydroxyproline determination was performed using a total collagen assay (QuickZyme Biosciences, The Netherlands) according to the manufacturer's instructions. Expression of the collagen genes COL1A1 and COL1A2 was performed by qPCR as described in section 9.3.

[0480] Ultrasound elastography is used as a reliable and reproducible noninvasive method to assess liver elasticity (stiffness) and has been shown to positively correlate with liver fibrosis (Li, R., Ren, X., Yan, F., et al. Liver fibrosis detection and staging: a comparative study of T1ρ MR imaging and 2D real-time shear-wave elastography. Abdom Radiol 43, 1713-1722 (2018). https: / / doi.org / 10.1007 / s00261-017-1381-3). In addition, this technology has been used in the clinic and may help better translate the results of preclinical data to human liver disease with fibrosis. Determination of liver stiffness using ultrasound-based shear wave elastography (SWE): using SWE was performed using a device (Supersonic Imagine, Aix-en-Provence, France). For acquisition, mice were anesthetized with isoflurane (about 1.5%) and placed on a heating pad. An ultrasound probe (model SL25-15, Supersonic Imagine, bandwidth 25 MHz, number of elements 256) was attached to a stand and approached the liver for evaluation. The probe allowed adequate penetration of the waves for B-mode and SWE acquisition.

[0481] To minimize motion artifacts due to breathing, elastograms were acquired during expiration. Three elastograms were acquired for each mouse and time point. The mean stiffness was then extracted from the three elastograms. Ultrasound examinations lasted approximately 5 minutes.

[0482] Example 13: Generation of C2-truncated MFG-E8 (EGF-C1) and HSA fusion (EGF-HSA-C1); expression and purification.

[0483] Methods for producing the proteins disclosed herein are described below.

[0484] DNA was synthesized at Gene Art (Regensburg, Germany) and cloned into mammalian expression vectors using restriction enzyme-ligated cloning techniques. The resulting plasmid was transfected into HEK293T cells to transiently express protein. In brief, polyethyleneimine (PEI; Catalog No. 24765 Polysciences, Inc.) was used to transfect the vector into HEK293T cells adapted to the suspension. Typically, 100 ml cells suspended at a density of 1-2 Mio cells / ml were transfected with DNA containing 100 μg of an expression vector encoding a protein of interest. The recombinant expression vector was then introduced into a host cell, and a construct was produced by further culturing the cell for 7 days to allow secretion into a culture medium (HEK, serum-free medium) supplemented with 0.1% pluronic acid, 4 mM glutamine, and 0.25 μg / ml antibiotics.

[0485] The resulting constructs were then purified from the cell-free supernatant using either immobilized metal ion affinity chromatography (IMAC) or anti-HSA capture chromatography.

[0486] When IMAC captures the histidine-tagged protein, the filtered conditioned medium is mixed with IMAC resin (Universal Health Medical Group) and equilibrated with 20 mM NaPO4, 0.5 Mn NaCl, 20 mM imidazole (pH 7.0). The resin is washed three times with 15 column volumes of 20 mM NaPO4, 0.5 Mn NaCl, 20 mM imidazole (pH 7.0), and then the protein is eluted with 10 column volumes of elution buffer (20 mM NaPO4, 0.5 Mn NaCl, 500 mM imidazole (pH 7.0)).

[0487] When the protein was captured by anti-HSA chromatography, the filtered conditioned medium was mixed with anti-HSA resin (Capture Select Human Albumin affinity matrix, Thermo) and equilibrated with PBS (pH 7.4). The resin was washed three times with 15 column volumes of PBS (pH 7.4), and then the protein was eluted with 10 column volumes of elution buffer (50 mM citrate, 90 mM NaCl (pH 2.5)) and the pH was neutralized with 1 M TRIS pH 10.0.

[0488] Finally, the eluted fractions were polished by using size exclusion chromatography (HiPrep Superdex 200, 16 / 60, General Life Sciences).

[0489] Throughout the purification process, aggregate content was followed by analytical size exclusion chromatography (Superdex 200 Increase 3.2 / 300 GL, General Life Sciences).

[0490] The aggregation level after the capture step and the expression yield after purification of C2 truncated MFG-E8 and HSA fusions are shown in Table 10. The HSA fusion of C2 truncated MFG-E8 showed at least a 40-fold improvement in expression over C2 truncated MFG-E8. In addition, the HSA fusion of C2 truncated MFG-E8 showed at least 4-fold less aggregation compared to C2 truncated MFG-E8. These data indicate that the HSA fusion of C2 truncated MFG-E8 exhibits better production characteristics compared to C2 truncated MFG-E8. Therefore, the HSA fusion appears to have better developability for use as a drug.

[0491] Table 10: Aggregation levels after capture step and expression yields after purification of EGF-C1 and EGF-HSA-C1 proteins

[0492]

[0493] Example 14: Dynamic Light Scattering (DLS) of C2 Truncated MFG-E8 (EGF-C1) and HSA Fusion (EGF-HSA-C1)

[0494] Aggregation propensity of C2-truncated MFG-E8 and HSA fusions was measured by dynamic light scattering (DLS, Wyatt). Dynamic light scattering is used to measure the translational diffusion coefficient of proteins in solution by quantifying the dynamic fluctuations in the scattered light. The DynaPro TM The plate reader (Wyatt Technologies Europe GmbH, Deinbach, Germany) in combination with the software DYNAMICS (version 7.1.0.25, Wyatt) measures the hydrodynamic radius as an indicator of aggregation formation at a concentration of 3 mg / ml under thermal stress. The protein solution is measured in a 384-well plate (384 round well plates, polystyrene, Thermo Fisher Scientific, Langenselbold, Germany).

[0495] like Fig.23As shown, C2-truncated MFG-E8 generally exhibited a higher hydrodynamic radius (5 nm compared to 80 nm at 25°C) compared to HSA fusions. In addition, the hydrodynamic radius of C2-truncated MFG-E8 increased significantly starting at 45°C, indicating strong aggregation, while HSA fusions maintained the same hydrodynamic radius until at least 55°C. These data indicate that HSA fusions of C2-truncated MFG-E8 are more stable and exhibit better biophysical properties compared to C2-truncated MFG-E8. Therefore, HSA fusions appear to have better developability for use as drugs.

[0496] Taken together, these data demonstrate that the fusion proteins of the disclosure, e.g., having an HSA domain insert, are functional and effective and therefore can be used as therapeutic agents.

[0497] It should be understood that the examples and embodiments described herein are for illustrative purposes only, and various modifications or changes will be apparent to those skilled in the art and are included within the spirit and scope of the present application and within the scope of the appended claims. All publications, patents, and patent applications cited herein are hereby incorporated by reference for all purposes.

Claims

1. A therapeutic fusion protein for enhancing efferocytosis, the therapeutic fusion protein comprising an integrin binding domain, a phosphatidylserine (PS) binding domain and a solubilization domain, wherein the fusion protein consists of an amino acid sequence selected from the group consisting of: - SEQ ID NO: 34, - SEQ ID NO: 73, or -SEQ ID NO:

147.

2. Use of the therapeutic fusion protein according to claim 1 in the preparation of a medicament for treating or preventing acute kidney injury or liver fibrosis.

Images