PCR polymerase chain reaction multi-well plate sealing equipment

Abstract

This invention relates to a PCR polymerase chain reaction (PCR) multiwell plate sealing device, comprising: a base plate, a first drive unit, a sliding base, a mold, a mounting base, a second drive unit, a swinging body, a roller, and a controller. The first drive unit is mounted on the base plate and connected to the sliding base; the mold is fixed to the sliding base. The mounting base is fixed to the base plate; the second drive unit is rotatably mounted on the mounting base; the swinging body is located at the front end of the second drive unit and rotatably mounted on the mounting base, and the second drive unit is connected to the swinging body. The movement trajectory of the sliding base is located below the swinging body; the roller is rotatably mounted on the sliding base; the first and second drive units are electrically connected to the controller. The PCR polymerase chain reaction (PCR) multiwell plate sealing device provided by this invention can reliably seal PCR multiwell plates and has a high degree of automation, significantly improving the efficiency of medical testing.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to a PCR polymerase chain reaction multi-well plate sealing device. Background Technology

[0002] PCR (polymerase chain reaction) is a method that uses a small amount of DNA as a template. With the participation of DNA polymerase and nucleotide substrates, this small amount of DNA is amplified to a quantity sufficient for functional analysis. PCR detection methods are of great significance in the rapid diagnosis of bacterial infectious diseases in clinical practice.

[0003] The PCR detection method is as follows: using oligonucleotide fragments as primers, DNA polymerase extends along the template strand according to the semi-conservative replication mechanism until new DNA is synthesized. Repeating this process can amplify the target DNA fragment, which can be simply regarded as DNA replication.

[0004] After collecting samples, PCR multiwell plates require sealing. Currently, the sealing equipment on the market has a low degree of automation and poor sealing effect. Summary of the Invention

[0005] This invention addresses the technical problems of low automation and poor sealing effect in existing sealing equipment by providing a PCR polymerase chain reaction multiwell plate sealing device.

[0006] The technical solution of the present invention to solve the above-mentioned technical problems is as follows:

[0007] A PCR polymerase chain reaction (PCR) multiwell plate sealing device includes:

[0008] Base plate, first drive unit, sliding base, mold, mounting base, second drive unit, swing body, roller and controller;

[0009] The first driving unit is mounted on the base plate and connected to the sliding base, and is used to drive the sliding base to reciprocate linear motion; the mold is fixed on the sliding base and is used to place a PCR polymerase chain reaction multi-well plate.

[0010] The mounting base is fixed on the base plate; the second drive unit is rotatably mounted on the mounting base; the swing body is disposed at the front end of the second drive unit, the swing body is rotatably mounted on the mounting base, and the second drive unit is connected to the swing body to drive the swing body to swing at a certain angle.

[0011] The movement trajectory of the sliding base is located below the swing body; the roller is rotatably mounted on the sliding base, and the roller is used to press the pressure-sensitive membrane onto the upper end of the PCR polymerase chain reaction multi-well plate;

[0012] The first drive unit and the second drive unit are electrically connected to the controller, respectively.

[0013] Furthermore: the first driving unit includes:

[0014] First motor, coupling, first bearing housing, first bearing, first nut, nut housing, ball screw, second bearing housing and second bearing;

[0015] The first bearing housing and the second bearing housing are arranged opposite to each other and fixed on the base plate; one end of the ball screw is connected to the first bearing in the first bearing housing, and the other end of the ball screw is connected to the second bearing in the second bearing housing; the output end of the first motor is connected to the front end of the ball screw through the coupling; the first nut is threadedly engaged with the ball screw and is fixed on the nut seat; the base plate is provided with a linear groove or linear slide rail, and the nut seat is limited to the linear groove or linear slide rail and can slide along the linear groove or linear slide rail; the sliding base is fixed on the nut seat.

[0016] Furthermore: the second drive unit includes:

[0017] Second motor, pressure screw, second nut and hinge;

[0018] The second motor is mounted on the mounting base via a rotating component; the output end of the second motor is connected to one end of the pressure screw, and the other end of the pressure screw is threadedly engaged with the second nut; the second nut is connected to the swing body via the hinge.

[0019] Furthermore: the swinging body is configured as an inverted triangular structure;

[0020] The roller is installed at the front end of the upper end of the swing body; a mounting groove is provided at the rear end of the upper end of the swing body, and the second nut is movably installed in the mounting groove through the hinge.

[0021] The lower end of the swing body is mounted on the mounting base via a rotating component.

[0022] Furthermore: the mounting base is configured with an L-shaped structure;

[0023] The second motor is mounted on the upper end of the mounting base via a rotating component, and the lower end of the swing body is mounted on the lower end of the mounting base via a rotating component.

[0024] Furthermore, it also includes: the outer casing;

[0025] The outer casing is fixed to the base plate;

[0026] The first drive unit, the mounting base, the second drive unit, the swing body, and the roller are disposed inside the housing; the front end of the housing is provided with an opening for the sliding base and the mold to pass through.

[0027] Furthermore, it also includes: protective shields;

[0028] The protective cover is configured as a flexible folding structure;

[0029] The front end of the protective cover is connected to the base plate, and the other end of the protective cover is connected to the sliding base; the ball screw is placed inside the protective cover.

[0030] Furthermore, it also includes: contact sensors;

[0031] The contact sensor is located at the rear end of the base plate and cooperates with the sliding base; the contact sensor is electrically connected to the controller.

[0032] Furthermore: the roller includes a shaft core;

[0033] The shaft core has a cylindrical structure;

[0034] The shaft core is covered with polyurethane antistatic adhesive.

[0035] Furthermore, the bottom of the base plate is symmetrically provided with multiple machine feet.

[0036] The PCR polymerase chain reaction multi-well plate sealing device provided by this invention has at least the following beneficial effects or advantages:

[0037] The PCR polymerase chain reaction (PCR) multi-well plate sealing device provided by this invention uses a first drive unit to drive a sliding base forward or backward. The sliding base drives a mold fixed to it and a PCR polymerase chain reaction multi-well plate fixed inside the mold forward or backward. A second drive unit drives a swinging body to swing at a certain angle in either the forward or reverse direction, thereby causing a roller mounted on the swinging body to move up or down a certain distance, thus adjusting the pressure of the pressure-sensitive membrane and ensuring reliable sealing of the PCR polymerase chain reaction multi-well plate. The PCR polymerase chain reaction multi-well plate sealing device provided by this invention uses a controller to automatically operate each component, achieving a high degree of automation and significantly improving the efficiency of medical testing. Attached Figure Description

[0038] Figure 1This is a diagram showing the internal structure of the PCR polymerase chain reaction multi-well plate sealing device provided in an embodiment of the present invention.

[0039] Figure 2 This is an external structural diagram of the PCR polymerase chain reaction multi-well plate sealing device provided in an embodiment of the present invention;

[0040] Figure 3 This is a schematic diagram illustrating the working principle of the PCR polymerase chain reaction multi-well plate sealing device provided in this embodiment of the invention;

[0041] Figure 4 This is a schematic diagram of the PCR polymerase chain reaction multiwell plate structure provided in an embodiment of the present invention.

[0042] The attached diagram lists the components represented by each number as follows:

[0043] 1-Second motor, 2-Swing body, 3-Roller, 4-Sliding base, 5-Mold, 6-Pressure-sensitive membrane, 7-First motor, 8-Mounting base, 9-Nut seat, 10-Ball screw, 11-Pressure film screw, 12-Contact sensor, 13-Protective cover, 14-Base plate, 15-Outer shell, 16-Second nut, 17-Coupling, 18-First bearing seat, 19-First nut, 20-Second bearing seat, 21-Machine foot. Detailed Implementation

[0044] This invention addresses the technical problems of low automation and poor sealing effect in existing sealing equipment by providing a PCR polymerase chain reaction multiwell plate sealing device.

[0045] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0046] In the description of this invention, it should be noted that the terms used in the various embodiments, such as "upper," "lower," "front," "rear," "left," and "right," which indicate orientation, are only used to simplify the description of the positional relationships based on the accompanying drawings and do not mean that the components and devices referred to must be operated in accordance with the specific orientations and defined operations, methods, and structures in the specification. Such directional terms do not constitute a limitation of this invention.

[0047] In the description of this invention, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "linking" should be interpreted broadly. For example, they can refer to fixed connections or detachable connections; they can refer to direct connections or indirect connections through an intermediate medium. Those skilled in the art can understand the specific meaning of the above terms in this invention in light of the specific circumstances.

[0048] This invention provides a PCR polymerase chain reaction (PCR) multi-well plate sealing device, such as... Figure 1 and Figure 2 It mainly includes: a base plate 14, a first drive unit (not shown in the figure), a sliding base 4, a mold 5, a mounting base 8, a second drive unit (not shown in the figure), a swing body 2, a roller 3, and a controller (not shown in the figure). Specifically:

[0049] The first drive unit is mounted on the base plate 14 and connected to the sliding base 4. The first drive unit is used to drive the sliding base 4 to reciprocate linearly. The mold 5 is fixed on the sliding base 4, and the upper end of the mold 5 is used to place a PCR polymerase chain reaction multiwell plate.

[0050] Mounting base 8 is fixed to base plate 14, and multiple feet 21 are symmetrically arranged on the bottom of base plate 14. The second drive unit is rotatably mounted on mounting base 8. In practical applications, the swing angle of the second drive unit relative to mounting base 8 is set to be less than 90°. Swing body 2 is located at the front end of the second drive unit and is rotatably mounted on mounting base 8. The second drive unit is connected to swing body 2 and is used to drive swing body 2 to swing at a certain angle, which is generally less than 90°.

[0051] The sliding base 4 moves below the swing body 2; the roller 3 is rotatably mounted on the sliding base 4 and is used to press the pressure-sensitive membrane 6 onto the upper end of the PCR polymerase chain reaction plate. The roller 3 includes a core; the core is cylindrical; the core is coated with polyurethane antistatic adhesive; the polyurethane antistatic adhesive is soft and antistatic, and this type of coated roller 3 compensates for the unevenness of the PCR reaction plate during the PCR sealing and rolling process, ensuring that some areas are not pressed properly.

[0052] The first and second drive units are electrically connected to the controller. The controller controls the operation of the first and second drive units to complete the sealing of the PCR polymerase chain reaction multi-well plate. The number of compressions and the pressure during the sealing process can be flexibly set as needed.

[0053] For example, this embodiment uses the following action process: Figures 1-4Initially, the sliding base 4 is positioned at the foremost position, facilitating the placement and removal of the PCR polymerase chain reaction (PCR) multiwell plate on the mold 5. The oscillating body 2 is raised upwards, allowing the roller 3 to align with the pressure-sensitive membrane 6 placed on the upper end of the PCR PCR multiwell plate. After the PCR PCR multiwell plate is placed on the mold 5, the controller controls the second drive unit to drive the oscillating body 2 to swing forward at a certain angle, causing the roller 3 to descend a certain distance. The roller 3 presses the pressure-sensitive membrane 6 against the upper end of the PCR PCR multiwell plate. Subsequently, the controller controls the first drive unit to drive the sliding base 4 to move backwards, and the PCR PCR multiwell plate moves backwards synchronously. During this movement, the roller 3 performs its first rolling action on the pressure-sensitive membrane 6 under the influence of friction. After the sliding base 4 moves backward to its limit position, the controller controls the second drive unit to continue driving the oscillating body 2 to swing forward at a certain angle, causing the roller 3 to descend a certain distance, increasing the pressure between the roller 3 and the pressure-sensitive membrane 6. The controller then controls the first drive unit to drive the sliding base 4 forward, and the PCR polymerase chain reaction multi-well plate moves forward synchronously. During the movement, the roller 3 rotates under the action of friction and performs a second rolling pressure on the pressure-sensitive membrane 6. After the sliding base 4 moves forward to its limit position, the controller controls the second drive unit to drive the oscillating body 2 to swing backward at a certain angle, returning to the initial position; then the sealed PCR polymerase chain reaction multi-well plate is removed.

[0054] like Figure 1 The mounting base 8 is configured as an L-shaped structure; the swing body 2 is configured as an inverted triangular structure; a roller 3 is installed at the front end of the upper end of the swing body 2; a mounting groove is opened at the rear end of the upper end of the swing body 2, and the second nut 16 is movably installed in the mounting groove through a hinge; the lower end of the swing body 2 is installed on the mounting base 8 through a rotating component.

[0055] In this embodiment, the first drive unit specifically adopts a motor-driven screw mechanism, as follows: Figure 1 and Figure 4The first drive unit includes: a first motor 7, a coupling 17, a first bearing housing 18, a first bearing, a first nut 19, a nut seat 9, a ball screw 10, a second bearing housing 20, and a second bearing. The first bearing housing 18 and the second bearing housing 20 are oppositely arranged and fixed on the base plate 14, and are aligned on the same straight line. One end of the ball screw 10 is connected to the first bearing in the first bearing housing 18, and the other end is connected to the second bearing in the second bearing housing 20. The ball screw 10 can rotate freely within the first and second bearings. The output end of the first motor 7 is connected to the front end of the ball screw 10 via the coupling 17; the first nut 19 is threadedly engaged with the ball screw 10 and is fixed on the nut seat 9. The base plate 14 is provided with a linear groove or linear slide rail, and the nut seat 9 is confined within the linear groove or linear slide rail, allowing it to slide along the linear groove or linear slide rail; a sliding base 4 is fixed on the nut seat 9. The output of the first motor 7 drives the ball screw 10 to rotate. During the rotation, the ball screw 10 drives the first nut 19 and the nut seat 9 to move forward or backward. The nut seat 9 drives the sliding base 4 to move forward or backward, thereby indirectly driving the mold 5 and the PCR polymerase chain reaction multi-well plate fixed above it to move forward or backward.

[0056] Similarly, in this embodiment, the second drive unit specifically adopts a motor-driven method in conjunction with a screw, specifically: as follows: Figure 1 and Figure 4 The second drive unit includes a second motor 1, a pressure screw 11, a second nut 16, and a hinge. The second motor 1 is mounted on the upper end of the L-shaped mounting base 8 via a rotating component, and can rotate slightly forward and backward on the mounting base 8. The output end of the second motor 1 is connected to one end of the pressure screw 11, and the other end of the pressure screw 11 is threaded into the second nut 16; the second nut 16 is connected to the swing body 2 via the hinge. The output end of the second motor 1 drives the pressure screw 11 to rotate, and the pressure screw 11 drives the second nut 16 to move forward or backward slightly during rotation. Since the second nut 16 is hinged to the swing body 2, and the lower end of the swing body 2 is movably connected to the mounting base 8, the second motor 1 rotates slightly as a whole to adjust its position during the rotation of the pressure screw 11, and the swing body 2 swings forward or backward at a certain angle, thereby adjusting the vertical position of the roller 3.

[0057] This embodiment provides a flat PCR polymerase chain reaction multi-well plate sealing device. To achieve protection of the core components and improve the aesthetics of the device, such as... Figure 2 and Figure 4It also includes: a housing 15 and a protective cover 13; the housing 15 is fixed to the base plate 14. The first drive unit, the mounting base 8, the second drive unit, the swing body 2, and the roller 3 are disposed inside the housing 15; the front end of the housing 15 is provided with an opening for the sliding base 4 and the mold 5 to pass through. The protective cover 13 is configured as a flexible folding structure; the front end of the protective cover 13 is connected to the base plate 14, and the other end of the protective cover 13 is connected to the sliding base 4; the ball screw 10 is placed inside the protective cover 13.

[0058] To accurately detect the position of the sliding base 4, such as Figure 2 and Figure 4 This embodiment also includes a contact sensor 12; the contact sensor 12 is disposed at the rear end of the base plate 14 and cooperates with the sliding base 4; the contact sensor 12 is electrically connected to the controller. When the forward movement contacts the contact sensor 12, the contact sensor 12 sends a feedback signal to the controller.

[0059] Therefore, the PCR polymerase chain reaction multi-well plate sealing device provided in this embodiment of the invention has at least the following beneficial effects or advantages:

[0060] The PCR polymerase chain reaction (PCR) multi-well plate sealing device provided in this invention uses a first drive unit to drive a sliding base forward or backward. The sliding base moves a mold fixed to it and the PCR PCR multi-well plate fixed inside the mold forward or backward. A second drive unit drives a swinging body to swing at a certain angle in either the forward or reverse direction, thereby causing a roller mounted on the swinging body to move up or down a certain distance, thus adjusting the pressure of the pressure-sensitive membrane and ensuring reliable sealing of the PCR PCR multi-well plate. The PCR PCR multi-well plate sealing device provided in this invention uses a controller to automatically operate each component, achieving a high degree of automation and significantly improving the efficiency of medical testing.

[0061] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A PCR polymerase chain reaction (PCR) multi-well plate sealing device, characterized in that: include: Base plate, first drive unit, sliding base, mold, mounting base, second drive unit, swing body, roller and controller; The first drive unit is mounted on the base plate and is connected to the sliding base to drive the sliding base to reciprocate linear motion. The mold is fixed on the sliding base and is used to place a PCR polymerase chain reaction multi-well plate. The mounting base is fixed on the base plate; the second drive unit is rotatably mounted on the mounting base; the swing body is disposed at the front end of the second drive unit, the swing body is rotatably mounted on the mounting base, and the second drive unit is connected to the swing body to drive the swing body to swing at a certain angle. The movement trajectory of the sliding base is located below the swing body; the roller is rotatably mounted on the sliding base, and the roller is used to press the pressure-sensitive membrane onto the upper end of the PCR polymerase chain reaction multi-well plate; The first drive unit and the second drive unit are electrically connected to the controller, respectively; The second drive unit includes: Second motor, pressure screw, second nut and hinge; The second motor is mounted on the mounting base via a rotating component; the output end of the second motor is connected to one end of the pressure screw, and the other end of the pressure screw is threaded into the second nut; the second nut is connected to the swing body via the hinge. The oscillating body is configured as an inverted triangular structure; The roller is installed at the front end of the upper end of the swing body; a mounting groove is provided at the rear end of the upper end of the swing body, and the second nut is movably installed in the mounting groove through the hinge. The lower end of the swing body is mounted on the mounting base via a rotating component.

2. The PCR polymerase chain reaction multi-well plate sealing device according to claim 1, characterized in that: The first driving unit includes: First motor, coupling, first bearing housing, first bearing, first nut, nut housing, ball screw, second bearing housing and second bearing; The first bearing housing and the second bearing housing are arranged opposite to each other and fixed on the base plate; one end of the ball screw is connected to the first bearing in the first bearing housing, and the other end of the ball screw is connected to the second bearing in the second bearing housing; the output end of the first motor is connected to the front end of the ball screw through the coupling; the first nut is threadedly engaged with the ball screw and is fixed on the nut seat; the base plate is provided with a linear groove or linear slide rail, and the nut seat is limited to the linear groove or linear slide rail and can slide along the linear groove or linear slide rail; the sliding base is fixed on the nut seat.

3. The PCR polymerase chain reaction multi-well plate sealing device according to claim 2, characterized in that: The mounting base is configured with an L-shaped structure; The second motor is mounted on the upper end of the mounting base via a rotating component, and the lower end of the swing body is mounted on the lower end of the mounting base via a rotating component.

4. The PCR polymerase chain reaction multi-well plate sealing device according to any one of claims 1-3, characterized in that: Also includes: shell; The outer casing is fixed to the base plate; The first drive unit, the mounting base, the second drive unit, the swing body, and the roller are disposed inside the housing; The front end of the outer casing is provided with an opening for the sliding base and the mold to pass through.

5. The PCR polymerase chain reaction multi-well plate sealing device according to any one of claims 2-3, characterized in that: Also includes: Protective shield; The protective cover is configured as a flexible folding structure; The front end of the protective cover is connected to the base plate, and the other end of the protective cover is connected to the sliding base; The ball screw is placed inside the protective cover.

6. The PCR polymerase chain reaction multi-well plate sealing device according to any one of claims 1-3, characterized in that: Also includes: Contact sensor; The contact sensor is located at the rear end of the base plate and cooperates with the sliding base. The contact sensor is electrically connected to the controller.

7. The PCR polymerase chain reaction multi-well plate sealing device according to any one of claims 1-3, characterized in that: The roller includes a shaft core; The shaft core has a cylindrical structure; The shaft core is covered with polyurethane antistatic adhesive.

8. The PCR polymerase chain reaction multi-well plate sealing device according to any one of claims 1-3, characterized in that: The bottom of the base plate is symmetrically provided with multiple machine feet.

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