Device and method for quickly separating Amblyseius cucumeris from breeding medium

Abstract

The present invention discloses a device and method for quickly separating Amblyseius cucumeris from a breeding medium, which comprises a chassis, a vibrating disk, a dry ice screen, a material screen, a funnel and a collection container; a vibrating motor is arranged inside the chassis, the vibrating disk is placed directly above the chassis, and the vibrating disk is fixedly connected with the vibrating motor, and the vibrating motor drives the vibrating disk to vibrate; the material screen is fixedly connected above the vibrating disk and vibrates with the vibrating disk; the dry ice screen is arranged directly above the material screen, the funnel is located directly below the material screen, and the collection container is arranged directly below the funnel. The structure of the present invention is reasonably designed, can effectively separate mites from the breeding medium, can quickly and effectively collect industrially cultivated Amblyseius cucumeris, and the collected mites are all surviving individuals with complete limbs, which is beneficial for mite breeders to conduct quality inspection and observation, and greatly improves the detection efficiency and accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of biological control, and particularly to a device and method for rapidly separating Amblyseius cucumeris from a breeding medium. Background Art

[0002] Amblyseius cucumeris plays an important role in the biological control of pests. It belongs to the family Phytoseiidae and the genus Amblyseius, and is widely distributed around the world. Amblyseius cucumeris was introduced into China in the 1980s. Currently, it has been used to control the damage of pests such as Panonychus citri, Tetranychus viennensis, Tetranychus truncatus, and Tetranychus turkestani on various crops such as citrus, cotton, fragrant pear, hops, peach, chestnut, and apple in more than 2,500 counties and cities in 32 provinces across the country. The biological control technology of "using mites to control mites" has been increasingly emphasized in the control of mites and pests. Among them, Amblyseius cucumeris has become the species with the most research and the highest degree of large-scale production in China since 2000. In order to ensure the stable and high yield of predatory mite breeding, it is necessary to continuously strengthen the level of automated operation in industrial production.

[0003] In the process of industrial breeding, the quality detection and standard of predatory mites are very important. The results of quality detection can directly reflect the production effect and benefit of breeding. Quality detection requires counting the number of Amblyseius cucumeris in a unit breeding medium, and the breeding medium is composed of wheat bran and flour mites. At present, the counting method is to observe the mites attached to the breeding medium under a microscope manually. The breeding medium and mites are mixed together, and it is often impossible to accurately identify and count them, which is very time-consuming. This method is relatively primitive and is not conducive to large-scale analysis of the breeding situation of Amblyseius cucumeris. Therefore, in view of the above factors, there is an urgent need to invent a device that can simply and quickly collect Amblyseius cucumeris in a unit weight of breeding medium, and improve the quality detection efficiency and accuracy of current industrial production of Amblyseius cucumeris.

[0004] Therefore, the existing detection technology has defects and needs to be further improved and developed. Summary of the Invention

[0005] In order to overcome the deficiencies in the prior art, the purpose of the present invention is to provide a device and method for separating Amblyseius cucumeris from a breeding medium, which has the characteristics of being fast, simple, and low-cost, so as to solve the problem that it is difficult for the human eye to detect Amblyseius cucumeris in the breeding medium in current industrial breeding quality inspection.

[0006] To achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A device for rapidly separating Amblyseius cucumeris from a breeding medium, which includes a chassis, a vibrating disk, a dry ice screen, a material screen, a funnel, and a collection container; both the dry ice screen and the material screen have a side wall around them to prevent the material from running off from the side;

[0008] The interior of the chassis is provided with a vibration motor. The vibrating disk is placed directly above the chassis, and the vibrating disk is fixedly connected to the vibration motor. The vibration motor drives the vibrating disk to vibrate;

[0009] The material screen is fixedly connected above the vibrating disk and vibrates with the vibrating disk;

[0010] The dry ice screen is arranged directly above the material screen, the funnel is located directly below the material screen, and the collection container is arranged directly below the funnel.

[0011] Four ends of the upper part of the vibration motor are respectively connected with springs, and the lower ends of the springs are respectively supported on the bottom of the chassis; The top surface of the vibration chassis has an opening, and the bottom of the vibration tray is fixedly welded to the vibration motor through three connecting pipes.

[0012] Furthermore, the dry ice screen is erected directly above the material screen through a dry ice bracket. The upper end of the dry ice bracket is fixedly connected to the dry ice screen, and the lower end of the dry ice bracket is fixedly connected to the top of the chassis.

[0013] Furthermore, studs arranged vertically are respectively detachably fixed on both sides of the top of the vibrating disk, and a pair of rotating latches are respectively threadedly connected to the studs on both sides. Fixed clamping plates are respectively fixed on the top surface and the bottom surface of the material screen, and the fixed clamping plates are squeezed up and down by the rotating latches on the studs on both sides to fix the material screen.

[0014] Furthermore, clamping grooves are respectively fixed on both sides of the top of the vibrating disk, and the studs on both sides are respectively inserted into the corresponding clamping grooves and fixed by tightening with threaded latches.

[0015] Furthermore, the funnel is erected directly below the material screen through a collection bracket. The upper end of the collection bracket is fixedly connected to the funnel, and the lower end of the collection bracket is fixedly connected to the top of the chassis.

[0016] Furthermore, a placement platform located below the funnel is fixed on the collection bracket, and the collection container is placed on the placement platform.

[0017] Furthermore, the collection container is a groove slide, the concave surface of the groove slide is square, the side length of the concave surface is 10 mm, and the depth of the concave surface is 3 mm.

[0018] Furthermore, the mesh number of the material screen is 45 meshes.

[0019] A separation method of the device for quickly separating Amblyseius cucumeris from the breeding medium according to the present invention includes the following steps:

[0020] 1) Place a unit weight of dry ice in the dry ice screen, and place the breeding medium to be measured with a unit weight in the material screen;

[0021] 2) The dry ice in the dry ice sieve sublimates, releasing low-temperature gas into the material sieve to freeze the breeding medium. The mites in the breeding medium are frozen and lose their adhesion. The freezing time is controlled at 4 - 5 minutes.

[0022] 3) After the freezing time ends, turn on the switch of the vibration motor. The vibration motor shakes the breeding medium in the material sieve on the vibration tray at high frequency. The vibration time of the vibration motor is controlled by a time relay for 18 - 22 seconds. After vibration filtration, the wheat bran in the breeding medium remains in the sieve, while the mites have a gravitational tendency and can pass through the material sieve. The mites on the breeding medium are collected in a collection container through a smooth funnel after freezing and vibration.

[0023] The present invention adopts the above technical solutions and has the following beneficial technical effects:

[0024] 1. The dry ice is placed in the hollow of the dry ice sieve, and the gas released downward by the gasification of the dry ice plays a freezing effect, freezing the mites in the breeding medium, causing the mites to lose their adhesion. Then, vibration is used to separate the breeding medium from the mites. The finally separated mites are collected using a grooved glass slide. The structure of the present invention is reasonably designed, can effectively separate the mites from the breeding medium, can quickly and effectively collect the Amblyseius cucumeris cultured industrially, and the collected mites are all surviving individuals with complete limbs, which is conducive to the quality inspection and observation of mite breeders, greatly improving the detection efficiency and accuracy.

[0025] 2. The collection container uses a grooved glass slide. The groove of the grooved glass slide can effectively prevent the mites from crawling out in a short time, and the groove size is suitable for the microscope observation imaging range available on the market, which is easy for manual later detection and observation.

[0026] 3. The whole device has a simple structure, the materials are easy to obtain, and the installation is simple and convenient. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The following further describes the present invention in detail in conjunction with the drawings and specific embodiments;

[0028] Figure 1 is a schematic diagram of the present invention;

[0029] Figure 2 is a schematic diagram of another perspective of the present invention;

[0030] Figure 3 is a schematic diagram of the grooved glass slide. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0031] Please refer to Figures 1-3, a device for quickly separating Amblyseius cucumeris from the breeding medium according to the present invention, which comprises a chassis 17, a vibrating disk 11, a dry ice screen 1, a material screen 5, a funnel 6 (with a smooth inner wall and a conical shape), and a collection container 7; the dry ice screen 1 and the material screen 5 are both provided with a side wall around the perimeter to prevent the material from escaping from the side.

[0032] A vibrating motor 15 is provided inside the chassis 17. The vibrating disk 11 is placed directly above the chassis 17. The top surface of the vibrating chassis 17 has an opening. The bottom of the vibrating disk 11 is fixedly welded to the vibrating motor 15 through three connecting pipes 16, so as to realize the vibration of the vibrating disk 11 driven by the vibrating motor 15; the top of the vibrating motor 15 has an upper polarization block 13, the bottom of the vibrating motor 15 has a lower polarization block 14, and the four ends of the upper part of the vibrating motor 15 are respectively connected with springs 12, and the lower ends of the springs 12 are respectively supported on the bottom of the chassis 17;

[0033] The material screen 5 is fixedly connected above the vibrating disk 11 and vibrates with the vibrating disk 11; the dry ice screen 1 is arranged directly above the material screen 5, the funnel 6 is located directly below the material screen 5, and the collection container 7 is arranged directly below the funnel 6.

[0034] The dry ice screen 1 is erected directly above the material screen 5 through a dry ice bracket 2. The upper end of the dry ice bracket 2 is fixedly connected with the dry ice screen 1, and the lower end of the dry ice bracket 2 is fixedly connected with the top of the chassis 17.

[0035] Vertically arranged studs 9 are respectively detachably fixed on both sides of the top of the vibrating disk 11. A pair of rotating latches 10 are respectively threadedly connected to the studs on both sides. Fixed clamping plates 3 and 4 are respectively fixed on the top surface and the bottom surface of the material screen 5. The fixed clamping plates 3 and 4 are squeezed up and down by the rotating latches 10 on the studs on both sides to fix the material screen 5. The detachable structure of the vibrating disk 11 and the stud 9 is specifically as follows: Slots are respectively fixed on both sides of the top of the vibrating disk 11. The studs 9 on both sides are respectively inserted into the corresponding slots and fixed by tightening with a threaded latch 18.

[0036] The funnel 6 is erected directly below the material screen 5 through a collection bracket 8. The upper end of the collection bracket 8 is fixedly connected with the funnel 6, and the lower end of the collection bracket 8 is fixedly connected with the top of the chassis 17. In addition, a placement platform is fixed on the collection bracket 8 and located below the funnel 6, and the collection container 7 is placed on the placement platform.

[0037] Preferably, the collection container 7 in the present invention adopts a grooved slide. The concave surface of the grooved slide is square, with a side length of 10 mm and a depth of 3 mm. The diameter of the material sieve mesh 5 is 10 cm, and the mesh number of the material sieve mesh 5 is 45 meshes. The diameter of the dry ice sieve mesh 1 is 8 cm. The dry ice sieve mesh 1 is placed 2 cm directly above the material sieve mesh 5, and the funnel 6 is placed 1 cm directly below the material sieve mesh 5. The round hole at the bottom of the funnel 6 is placed 5 mm directly above the concave surface of the grooved slide.

[0038] The separation method of the present invention includes the following steps:

[0039] 1) Place a unit weight of dry ice in the dry ice sieve mesh 1, and place the feeding medium to be measured with a unit weight in the material sieve mesh 5;

[0040] 2) The dry ice in the dry ice sieve mesh 1 sublimes to release low-temperature gas into the material sieve mesh 5 to freeze the feeding medium. The mites in the frozen feeding medium lose their adhesion, and the freezing time is controlled within 4 - 5 min;

[0041] 3) After the freezing time ends, turn on the switch of the vibration motor 15. The vibration motor 15 shakes the feeding medium in the material sieve mesh 5 on the vibration tray at a high frequency. The vibration time of the vibration motor 15 is controlled by a time relay for 18 - 22 s. After vibration filtration, the wheat bran in the feeding medium remains in the sieve mesh, while the mites have a gravitational tendency and can pass through the material sieve mesh 5. The mites on the feeding medium are concentrated and collected in the collection container 7 through the smooth funnel 6 after freezing and vibration; then remove the grooved slide to obtain the Amblyseius cucumeris in the whole unit weight of the feeding medium.

[0042] The above describes the implementation of the present invention in conjunction with the drawings, but the present invention is not limited to the above specific implementation manners. The above specific implementation manners are illustrative rather than restrictive of the present invention. Those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered within the scope of the claims and the description of the present invention.

Claims

1. A device for quickly separating Amblyseius cucumeris from the breeding medium, characterized in that: It includes a chassis, a vibrating disk, a dry ice screen, a material screen, a funnel and a collection container; A vibrating motor is provided inside the chassis. The vibrating disk is placed directly above the chassis and is fixedly connected to the vibrating motor. The vibrating motor drives the vibrating disk to vibrate; The material screen is fixedly connected above the vibrating disk and vibrates with the vibrating disk; The dry ice screen is arranged directly above the material screen. The funnel is located directly below the material screen. The collection container is arranged directly below the funnel. The dry ice in the dry ice screen sublimes to release low-temperature gas into the material screen to freeze the breeding medium. The mites in the frozen breeding medium lose their adhesion. The freezing time is controlled within 4-5 minutes; The dry ice screen is erected 2 cm directly above the material screen through a dry ice support. The upper end of the dry ice support is fixedly connected to the dry ice screen, and the lower end of the dry ice support is fixedly connected to the top of the chassis. The diameter of the dry ice screen is 8 cm. On both sides of the top of the vibrating disk, vertically arranged studs are respectively detachably fixed. A pair of rotating latches are respectively threadedly connected to the studs on both sides. Fixed clamping plates are respectively fixed on the top surface and the bottom surface of the material screen. The material screen is fixed by the rotating latches on the studs on both sides pressing the fixed clamping plates up and down. The collection container is a grooved glass slide. The concave surface of the grooved glass slide is square, the side length of the concave surface is 10 mm, and the depth of the concave surface is 3 mm. The diameter of the material screen is 10 cm, and the mesh number of the material screen is 45 meshes. The funnel is placed 1 cm directly below the material screen, and the round hole at the bottom of the funnel is placed 5 mm directly above the concave surface of the grooved glass slide; Among them, the breeding medium in the material screen on the vibrating tray is shaken at a high frequency by the vibrating motor. The vibrating time of the vibrating motor is controlled by a time relay for 18-22 s. After vibration filtration, the wheat bran in the breeding medium remains in the screen, while the mites have a gravitational tendency and can pass through the material screen. The mites on the breeding medium are collected in the collection container through the smooth funnel after freezing and vibration. The grooved glass slide is removed to obtain the Amblyseius cucumeris in the breeding medium per unit weight.

2. The device for quickly separating Amblyseius cucumeris from the breeding medium according to claim 1, characterized in that: Four ends of the upper part of the vibrating motor are respectively connected with springs, and the lower ends of the springs are respectively supported on the bottom of the chassis; The top surface of the chassis has an opening. The bottom of the vibrating tray is fixedly welded to the vibrating motor through three connecting pipes.

3. The device for quickly separating Amblyseius cucumeris from the breeding medium according to claim 1, characterized in that: On both sides of the top of the vibrating disk, clamping grooves are respectively fixed. The studs on both sides are respectively inserted into the corresponding clamping grooves and fixed by tightening with threaded latches.

4. The device for quickly separating Amblyseius cucumeris from the breeding medium according to claim 1, characterized in that: The funnel is erected directly below the material screen through a collection support. The upper end of the collection support is fixedly connected to the funnel, and the lower end of the collection support is fixedly connected to the top of the chassis.

5. The device for quickly separating Amblyseius cucumeris from the breeding medium according to claim 4, characterized in that: A placement platform is fixed on the collection support and is located below the funnel. The collection container is placed on the placement platform.

6. The separation method of the device for quickly separating Amblyseius cucumeris from the breeding medium according to any one of claims 1-5, characterized in that: It includes the following steps: 1) Place a unit weight of dry ice in the dry ice screen and place the breeding medium to be measured per unit weight in the material screen; 2) The dry ice in the dry ice screen sublimes to release low-temperature gas into the material screen to freeze the breeding medium. The mites in the frozen breeding medium lose their adhesion. The freezing time is controlled within 4-5 minutes; 3) After the freezing time ends, turn on the switch of the vibration motor. The vibration motor shakes the feeding medium in the material sieve on the vibration tray at a high frequency. The vibration time of the vibration motor is controlled by a time relay for 18 - 22 s. After vibration filtration, the wheat bran in the feeding medium remains in the sieve, while the mites have a gravitational tendency and can pass through the material sieve. The mites on the feeding medium are collected in the collection container through a smooth funnel after freezing and vibration.

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