A gene extraction module and a gene detection device

Abstract

The embodiment of the application provides a gene extraction module and a gene detection device, and belongs to the technical field of gene detection. The gene extraction module comprises a base, a traction mechanism and a nucleic acid extraction mechanism. The base comprises an extraction channel extending along a first direction. The extraction channel comprises, in the first direction, a placement station, an extraction station, a product transfer station and a collection station in sequence. The placement station is used for placing a reagent box. The extraction station is used for nucleic acid extraction. The product transfer station is used for transferring the extracted product. The collection station is used for collecting the reagent box after the product transfer is completed. The traction mechanism is arranged on the base. The traction mechanism is configured to move the reagent box in the extraction channel along the first direction, so that the reagent box passes through the extraction station and the product transfer station in sequence from the placement station to the collection station. The gene extraction module can improve the detection efficiency of genes.

Description

Technical Field

[0001] This application relates to the field of gene detection technology, and more specifically, to a gene extraction module and a gene detection device. Background Technology

[0002] Genetic testing involves sample collection and gene processing. It is typically performed in specialized PCR laboratories, which are space-consuming and have low automation levels, requiring significant human intervention. While some gene analysis devices are available that can achieve "sample in, result out," these devices require manual opening of the sample tube caps before loading, increasing the workload for operators, posing a risk of infection, and increasing the risk of human error affecting test results. Furthermore, nucleic acid extraction is limited to a single extraction station, allowing only one sample at a time. Therefore, existing gene testing equipment suffers from low efficiency. Summary of the Invention

[0003] This application provides a gene extraction module and a gene detection device to improve the efficiency of gene detection.

[0004] In a first aspect, embodiments of this application provide a gene extraction module, which includes a base, a traction mechanism, and a nucleic acid extraction mechanism. The base includes an extraction channel extending along a first direction, wherein the extraction channel sequentially includes a placement station, an extraction station, a product transfer station, and a collection station in the first direction. The placement station is used to place a reagent kit, the extraction station is used for nucleic acid extraction, the product transfer station is used to transfer the extracted product, and the collection station is used to collect the reagent kit after product transfer. The traction mechanism is disposed on the base and configured to traction the reagent kit to move along the first direction in the extraction channel, so that the reagent kit moves from the placement station, sequentially through the extraction station and the product transfer station, to the collection station. The nucleic acid extraction mechanism is disposed at the extraction station and is used to extract nucleic acids from the sample in the reagent kit.

[0005] In the above technical solution, the gene extraction module adopts a streamlined nucleic acid extraction mode. The test kit is placed at the placement station of the base, and samples and reagents are added to the kit. Then, driven by the traction mechanism, the kit can be transferred from the placement station to the extraction station for nucleic acid extraction. The extraction station is equipped with a nucleic acid extraction mechanism to perform normal nucleic acid extraction steps on the kit, such as lysis, washing, and elution. After the nucleic acid separation in the kit is completed, the kit is moved from the extraction station to the product transfer station by the traction mechanism. At the product transfer station, the nucleic acid products in the kit are transferred to the subsequent PCR system construction area. Then, under the action of the traction mechanism, the kit is moved from the product transfer station to the collection station, where the kit can be transferred and collected after product transfer. Therefore, in this solution, the traction mechanism set on the base allows the reagent kit to form a complete nucleic acid extraction process on the extraction channel of the base, which greatly improves the continuity of nucleic acid extraction in the gene extraction module. That is, the series of operations from reagent kit placement, sample addition, reagent addition, nucleic acid extraction, product transfer, and reagent kit transfer and collection are all carried out on the extraction channel of the base and are achieved by the traction action of the traction mechanism. Furthermore, while the previous reagent kit is performing nucleic acid extraction at the extraction station, the next reagent kit at the placement station is already ready for sample addition. After the nucleic acid extraction of the previous reagent kit is completed, the next reagent kit located at the placement station can immediately enter the extraction station for nucleic acid extraction under the action of the traction mechanism. There is no interruption in the middle, the continuity is strong, and the extraction efficiency of nucleic acid is improved.

[0006] In addition, the gene extraction module provided in this application embodiment also has the following additional technical features:

[0007] In some embodiments, the extraction channel includes two sidewalls opposite each other along a second direction, the second direction being perpendicular to the first direction, and a guide groove extending along the first direction is provided on the sidewalls for guiding the reagent kit to move within the extraction channel.

[0008] In the above technical solution, a guide groove is formed on the side wall of the base. The guide groove can provide guidance and support for the movement of the reagent kit in the extraction channel, thereby ensuring the stability and orientation of the reagent kit during the movement process in the extraction channel. This makes the nucleic acid extraction process of the reagent kit in the extraction channel more stable and reliable, thereby reducing the probability of reagent spillage during the movement of the reagent kit in the extraction channel.

[0009] In some embodiments, the traction mechanism includes a gripping unit and a first driving component. The gripping unit is movably disposed on the base along the first direction for gripping and releasing the reagent kit. The first driving component is disposed on the base for driving the gripping unit to reciprocate on the base along the first direction.

[0010] In the above technical solution, the grasping unit can move the reagent kit directionally along the extraction channel, thereby enabling the reagent kit to complete nucleic acid extraction and product transfer processes sequentially.

[0011] In some embodiments, the gripping unit includes a movable member, a claw portion, and an elastic member. The movable member is movably disposed on the base along the first direction. The claw portion is rotatably connected to the movable member and has a guide slope and a locking groove. The elastic member is disposed between the claw portion and the movable member and is used to apply an elastic force to the claw portion. When the movable member moves to the point where the guide slope of the claw portion contacts the locking portion of the reagent kit, the locking portion can squeeze the claw portion and drive the claw portion to rotate, so that the elastic member accumulates elastic potential energy. The elastic potential energy is used to drive the claw portion to rotate and reset when the locking groove engages with the locking portion, so that the locking portion engages with the locking groove.

[0012] In the above technical solution, the gripper and moving part in the gripping unit are rotated. When the moving part moves to the point where the guide slope of the gripper contacts the locking part on the reagent kit at the placement station, the reagent kit remains stationary. The moving part squeezes the reagent kit, i.e., the gripper squeezes the locking part. The gripper and moving part rotate in coordination, and the guide slope on the gripper guides the gripper, thus guiding the gripper to rotate. At this time, the elastic element is compressed, thereby accumulating elastic potential energy. The moving part continues to squeeze the locking part until the locking groove on the gripper engages in the locking part. At this time, the elastic potential energy is released and acts on the gripper to rotate and reset, so that the locking part engages in the locking groove. This achieves automatic squeezing and locking of the gripper and the locking part on the reagent kit without human intervention. It can automatically connect the gripping unit and the reagent kit, with a high degree of automation. In this way, the gripping unit can move with the reagent kit in the placement station, extraction station, product transfer station and collection station in the extraction channel.

[0013] In some embodiments, the first drive assembly includes a first drive member, a pulley, and a timing belt. The first drive member is mounted on the base and is used to drive the pulley to rotate. The timing belt is driven in conjunction with the pulley and extends along the first direction. The moving member is connected to the timing belt and moves along the first direction on the base following the timing belt under the action of the first drive member.

[0014] In the above technical solution, by setting the first driving component as a synchronous belt driving method, the area occupied is smaller, the structure is more compact, and the synchronous belt driving method is stable and reliable. The first driving component can be set on the outside of the extraction channel on the base, and the synchronous belt can be set along the top of the base, so that the first driving component will not occupy the internal space of the extraction channel, which facilitates the spatial arrangement of the nucleic acid extraction mechanism.

[0015] In some embodiments, the base is provided with an extraction chamber at the extraction station, the nucleic acid extraction mechanism is disposed in the extraction chamber, and the extraction chamber is used to separate the placement station from the product transfer station.

[0016] In the above technical solution, by setting an extraction chamber on the base, the extraction chamber can isolate the placement station and the product transfer station, so that the inside of the extraction chamber is in a relatively sealed environment, reducing the probability of gas leakage during nucleic acid extraction, thus making the nucleic acid extraction process safer and more environmentally friendly.

[0017] In some embodiments, the extraction chamber has clearance openings formed on both sides in the first direction between the bottom wall of the extraction channel and the clearance openings for the reagent kit to enter or leave the extraction chamber.

[0018] In the above technical solution, by setting up an avoidance opening, it is convenient for the reagent kit to enter or leave the extraction chamber, thereby carrying out the corresponding nucleic acid extraction operation.

[0019] In some embodiments, the extraction chamber is provided with a door assembly at the avoidance opening, the door assembly being used to open and close the avoidance opening.

[0020] In the above technical solution, since some harmful gases are generated during the nucleic acid extraction process, these gases will escape from the avoidance opening to the outside of the extraction chamber, thus affecting the external air environment. Therefore, by setting a door assembly at the avoidance opening, the avoidance opening can be closed and opened. Opening the avoidance opening makes it easier for the reagent kit to enter or leave the extraction chamber. During the nucleic acid extraction process, the avoidance opening is closed by the door assembly, which can effectively reduce the gas leakage generated during the nucleic acid extraction process, ensure the airtightness of the extraction chamber in the gene extraction module, and effectively ensure the external air environment.

[0021] In some embodiments, the hatch assembly includes a hatch and a lifting assembly. The hatch is used to close the clearance opening. The lifting assembly is disposed in the extraction chamber and is used to drive the hatch to move along a third direction, which is perpendicular to the first direction and the second direction.

[0022] In the above technical solution, the lifting assembly can drive the door to move along a third direction on the extraction chamber. When it is necessary to open the clearance opening, the lifting assembly drives the door to move away from the bottom wall of the base, thus exposing the clearance opening and facilitating the entry or exit of the reagent kit into or out of the extraction chamber. After the reagent kit enters the extraction chamber, the lifting assembly drives the door to move closer to the bottom wall, thereby closing the clearance opening. This can prevent the gas generated during the nucleic acid extraction process in the extraction chamber from leaking out, thus ensuring the external air environment of the gene extraction module.

[0023] In some embodiments, the extraction chamber is provided with a second guide rail, and the door is provided with a slider on the side near the extraction chamber, the slider being slidably engaged with the second guide rail.

[0024] In the above technical solution, the second guide rail slides in conjunction with the slider on the hatch, and the second guide rail can guide the hatch to move up and down, thereby orienting the direction of the hatch's movement and ensuring the stability of the hatch during the up and down movement.

[0025] In some embodiments, the extraction channel is provided with a sensing sensor on the side near the extraction chamber, the sensing sensor being used to control the operation of the door assembly when the moving part enters or leaves the extraction station.

[0026] In the above technical solution, a sensing sensor is installed on the side of the extraction channel near the extraction chamber. This sensor can control the door assembly based on the received signals, resulting in a higher degree of automation for the door assembly, eliminating the need for human intervention and enabling automatic control. Specifically, when a moving part in the traction mechanism approaches the extraction chamber, the sensing sensor detects the moving part and sends a command back to the control module. The control module then controls the door to open, facilitating the movement of the moving part through the extraction chamber.

[0027] In some embodiments, the nucleic acid extraction mechanism includes a fixed frame, a movable frame, a magnetic rod mounting frame, a magnetic rod sleeve mounting frame, a second drive assembly, a third drive assembly, and a fourth drive assembly. The fixed frame is mounted on the base and located within the extraction chamber. The movable frame is movably mounted on the fixed frame along a first direction. The magnetic rod mounting frame is movably mounted on the movable frame along a third direction, and a magnetic rod is mounted on the magnetic rod mounting frame. The third direction is perpendicular to the first and second directions. The magnetic rod sleeve mounting frame is movably mounted on the movable frame along a third direction. In the third direction, the magnetic rod sleeve mounting frame is closer to the extraction chamber than the magnetic rod mounting frame. The system includes a channel; a second driving component is mounted on the movable frame, which drives the magnetic rod mounting frame to move along the third direction; a third driving component is mounted on the movable frame, which drives the magnetic rod mounting frame to move along the third direction; a fourth driving component is mounted on the fixed frame, which drives the movable frame to move along the first direction on the fixed frame; wherein, under the driving action of the second driving component and the third driving component, the magnetic rod can be inserted into the magnetic rod sleeve and can be inserted into the reagent kit located at the extraction station for magnetic attraction.

[0028] In the above technical solution, the magnetic rod and the magnetic rod sleeve work together. The magnetic rod can extend out of the magnetic rod sleeve. Under the action of the second and third driving components, the magnetic rod sleeve and the magnetic rod can be driven to move in a third direction and extend into the corresponding chamber of the reagent kit. By inserting the magnetic rod into the magnetic rod sleeve and extending into the chamber of the reagent kit together, the magnetic beads in the reagent kit can be adsorbed onto the outer surface of the magnetic rod sleeve to transfer the magnetic beads. When the magnetic rod is withdrawn from the magnetic rod sleeve, the magnetic beads can be re-dissolved in the lysis buffer or washing buffer. Therefore, by using the magnetic rod sleeve and the magnetic rod together, the transfer of nucleic acids attached to the magnetic beads in each chamber of the reagent kit can be realized.

[0029] Through the sliding engagement of the fixed frame and the movable frame, the movable frame, under the action of the fourth driving component, allows the magnetic rod fixing frame and the magnetic rod sleeve mounting frame to move along the first direction within the extraction chamber. This allows the magnetic rod, in conjunction with the magnetic rod sleeve, to perform corresponding nucleic acid extraction operations on each reagent cavity of the reagent kit. Furthermore, by keeping the reagent kit stationary within the extraction chamber while the nucleic acid extraction mechanism moves within it, the nucleic acid extraction operation on each reagent cavity of the reagent kit is gradually achieved. This allows for a more compact internal space layout within the nucleic acid chamber, requiring only a space slightly larger than the length of the reagent kit in the first direction. Since the reagent kit does not need to move, the internal space arrangement of the extraction chamber is more rational, resulting in a smaller extraction chamber volume.

[0030] Specifically, the magnetic nanobeads are pre-placed in the lysis chamber of the kit. The magnetic rod's function is to adsorb and capture the DNA nanobeads during extraction. After the extracted DNA is captured by the magnetic nanobeads, the second and third driving components operate, driving the magnetic rod and its sleeve to descend together into the lysis wells of the kit. Due to the strong magnetic force of the magnetic rod, all the magnetic nanobeads are adsorbed onto the outer surface of the sleeve. After a certain period of adsorption, the magnetic rod and sleeve rise simultaneously, then descend again into the washing well. Subsequently, the magnetic rod rises under the action of the second driving component. As the magnetic rod inside the sleeve is removed, the magnetic nanobeads attached to the surface of the sleeve are re-dissolved in the washing solution, washing away the nucleic acids on the beads. After washing, when transferring the beads to the next washing well, the magnetic rod descends and re-inserts into the sleeve. The magnetic rod, inserted in the sleeve and simultaneously descending into the washing well, adsorbs the beads, then rises together, then moves horizontally and descends to another washing well, thus achieving the transfer of magnetic beads between the various chambers within the kit.

[0031] In some embodiments, the magnetic rod sleeve mounting bracket is provided with a socket for inserting the magnetic rod sleeve. Under the action of the third driving component, the magnetic rod sleeve mounting bracket can be inserted into the magnetic rod sleeve located in the reagent kit, so that the magnetic rod sleeve located in the reagent kit is installed on the magnetic rod sleeve mounting bracket.

[0032] In the above technical solution, since the magnetic rod sleeve is a disposable consumable and can only be used for the same sample, in the initial state, the magnetic rod sleeve is located in the magnetic rod sleeve hole of the reagent kit. Then, when the reagent kit enters the extraction chamber under the action of the traction mechanism, the magnetic rod sleeve frame is driven to descend under the action of the third driving component, and is installed on the magnetic rod sleeve frame with the magnetic rod sleeve in the reagent kit, which facilitates the magnetic attraction of the magnetic beads. Therefore, by using a magnetic rod sleeve that comes with the reagent kit, each sample can correspond to one magnetic rod sleeve, avoiding cross-contamination caused by the magnetic rod sleeve combining with the reagent solution, and ensuring the accuracy of the results during nucleic acid extraction.

[0033] In some embodiments, the depth direction of the insertion hole extends along the third direction, the inner wall of the insertion hole is provided with a limiting groove, and the outer wall of the magnetic rod sleeve is provided with a limiting protrusion that cooperates with the limiting groove, so as to restrict the axial movement of the magnetic rod sleeve on the magnetic rod sleeve mounting bracket.

[0034] In the above technical solution, a limiting groove is provided on the inner wall of the insertion hole, and a matching limiting protrusion is provided on the outer wall of the magnetic rod sleeve. When the magnetic rod sleeve holder descends to be inserted into the magnetic rod sleeve in the reagent kit, the limiting protrusion on the outer wall of the magnetic rod sleeve can be inserted into the limiting groove on the insertion hole, thereby installing the magnetic rod sleeve on the magnetic rod holder and restricting the axial movement of the magnetic rod sleeve on the magnetic rod sleeve mounting frame. When it is necessary to remove the magnetic rod sleeve, simply insert the magnetic rod into the magnetic rod sleeve and push the magnetic rod sleeve downwards to separate the magnetic rod sleeve from the magnetic rod holder, thereby achieving the disassembly of the magnetic rod sleeve, which is then removed from the magnetic rod holder.

[0035] In some embodiments, the magnetic rod mounting bracket has multiple insertion holes, which are arranged at intervals along the second direction; the magnetic rod mounting bracket has multiple magnetic rods, and the number and position of the multiple magnetic rods correspond one-to-one with the number and position of the multiple insertion holes.

[0036] In the above technical solution, the reagent kit can hold multiple sets of nucleic acid samples to be tested. Correspondingly, by setting the number of insertion holes of the magnetic rod holder in the nucleic acid extraction mechanism to be multiple, corresponding to the number and position of the magnetic rods on the magnetic rod holder, the nucleic acid extraction mechanism can simultaneously extract nucleic acid from multiple samples in the reagent kit, which greatly improves the extraction efficiency of nucleic acid extraction.

[0037] In some embodiments, the nucleic acid extraction mechanism further includes a magnetic rod sleeve detection component, which is disposed on the movable frame and is used to detect the presence of the magnetic rod sleeve on the magnetic rod sleeve mounting frame.

[0038] In the above technical solution, since the magnetic rod sleeves on the magnetic rod sleeve mounting frame are automatically installed, a magnetic rod sleeve detection component is set on the movable frame to detect whether the magnetic rod sleeves are installed on the magnetic rod sleeve mounting frame. If the magnetic rod sleeves are not successfully installed on the magnetic rod sleeve mounting frame, it will affect the subsequent nucleic acid extraction operation. Therefore, the magnetic rod sleeve detection component is used to detect this, thereby ensuring that the magnetic rod sleeves on the magnetic rod sleeve mounting frame can be used for nucleic acid extraction after installation.

[0039] In some embodiments, the gene extraction module further includes a heating element disposed at the extraction station, the heating element being used to heat at least a portion of the bottom of the reagent kit.

[0040] In the above technical solution, since both the lysis and elution reactions in the nucleic acid extraction process require heating to promote them, a heating element is provided at the extraction station. This heating element can heat the corresponding parts at the bottom of the kit (i.e., the lysis wells and elution wells), thereby ensuring the normal progress of nucleic acid extraction within the kit.

[0041] In some embodiments, the heating element is movably disposed within the extraction channel along a third direction, which is perpendicular to the first direction and the second direction.

[0042] In the above technical solution, in order to ensure the heating element heats the bottom of the reagent kit and dissipates heat quickly after heating, the heating element is positioned both close to and away from the reagent kit. When heating the bottom of the reagent kit is required, the heating element is brought close to and in contact with the reagent kit to heat the corresponding part of the kit. After heating the bottom of the reagent kit, the heating element is moved away from the kit to allow for rapid heat dissipation. Compared to automatic cooling of the heating element, keeping the heating element away from the kit avoids the residual heat on the heating element from negatively affecting the nucleic acid sample in the reagent kit chamber, thus improving the accuracy of nucleic acid extraction.

[0043] In some embodiments, the heating element has a first position in contact with the bottom of the reagent kit and a second position separate from the bottom of the reagent kit. The gene extraction module further includes a fifth driving component mounted on the side wall, the fifth driving component being used to drive the heating element to switch between the first position and the second position.

[0044] In the above technical solution, by providing a fifth driving component, the heating element can be driven to move between a first position and a second position. When the reagent kit needs to be heated, the fifth driving component moves the heating element from the second position to the first position, so that the heating element contacts the bottom of the reagent kit and heats the corresponding chamber. After the corresponding chamber of the reagent kit has been heated, the fifth driving component drives the heating element to switch from the first position to the second position, allowing the heating element to separate from the reagent kit and canceling the heating of the reagent kit. Therefore, by providing a fifth driving component, the heating element can be moved closer to or away from the reagent kit, realizing automatic heating of the reagent kit without the need for manual operation of the heating element, resulting in a higher degree of automation.

[0045] In some embodiments, the fifth drive assembly includes a cam and a fifth drive member, the cam contacting the bottom of the heating element; the fifth drive member is mounted on the side wall and is used to drive the cam to rotate, so that the heating element switches between the first position and the second position.

[0046] In the above technical solution, by adopting a cam mechanism for the fifth driving component, the cam is driven to rotate under the action of the fifth driving component. The cam is always in contact with the bottom of the heating element. Under the rotation of the cam, the heating element can switch between the first position and the second position. When the heating element is in the first position, the heating element is in contact with the bottom of the reagent kit, thereby heating the corresponding chamber at the bottom of the reagent kit. When the cam continues to rotate until the heating element is no longer in contact with the reagent kit, the heating element stops heating the reagent kit.

[0047] The test kits located at the collection station can be transferred to other locations for collection, or they can be collected directly at the collection station.

[0048] In some embodiments, the collection station is equipped with a transfer mechanism, and the gene extraction module further includes a collection unit. The transfer mechanism is used to transfer the reagent kit after product transfer to the collection unit. Thus, the reagent kit located at the collection station can be transferred to the collection unit for collection by a transfer mechanism such as a robotic arm.

[0049] In some embodiments, the collection station is provided with a collection mechanism for collecting reagent kits. The collection mechanism includes a collection bucket located below the collection station, which is used to collect the reagent kits after product transfer. One end of the guide channel extends between the placement station and the extraction station, and the other end of the guide channel extends between the product transfer station and the collection station. When the reagent kit moves to the collection station, the reagent kit detaches from the guide channel and falls into the collection bucket under the action of gravity.

[0050] In the above technical solution, the guide groove disconnects at the collection station. When the reagent kit, after product transfer, is about to enter the collection station, the gripping unit pulls the reagent kit away from the guide groove. After one side of the reagent kit loses the support of the guide groove, it detaches from the guide groove and falls into the collection bucket under gravity, thus automatically completing the collection of the reagent kit. Therefore, the gene extraction module can complete the entire production line operation of reagent kit addition, extraction, transfer, and collection without the need for robotic arms or other transfer auxiliary mechanisms to transfer the reagent kit to other stations, resulting in a higher degree of structural integration for the gene extraction module. In some embodiments, the base has a collection cavity communicating with the outside at its bottom wall, the collection bucket is located within the collection cavity, and the bottom wall has an opening at the collection cavity communicating with the extraction channel. The collection mechanism also includes a sliding door assembly, movably disposed at the opening, used to close and open the opening.

[0051] In the above technical solution, by setting an opening in the bottom wall of the base and placing the collection bucket below the opening, and then using a sliding door assembly to close and open the opening, the airtightness of the extraction channel inside the base can be ensured.

[0052] In some embodiments, the sliding door assembly includes a sliding door and a mating part. The sliding door is slidably disposed at the opening along the first direction. The side wall is provided with a groove extending along the first direction near the bottom wall. The groove slidably engages with the sliding door. The sliding door has a closed state and an open state relative to the opening. The mating part is disposed on the sliding door. The moving member is provided with a pushing part that engages with the mating part. Under the movement of the moving member, the moving member pushes the mating part through the pushing part and drives the sliding door to slide along the first direction, so that the sliding door switches from the closed state to the open state.

[0053] In the above technical solution, a mating part is provided on the sliding door, and a pushing part is provided on the moving part. During the process of the moving part moving from the product transfer station to the collection station, the pushing part on the moving part can cooperate with the mating part. The moving part will push the mating part and drive the sliding door to move, so that the opening will open automatically. At this time, the reagent kit slides out of the guide groove. After the reagent kit separates from the hook part on the gripping unit, the reagent kit will fall into the collection bucket from the opening under the action of gravity. Therefore, in this solution, the sliding door can be automatically opened by the linkage action of the moving part on the traction mechanism, without the need for other drive structures or control mechanisms to open the sliding door, making the structure simpler and more compact.

[0054] In some embodiments, a reset member is provided between the sliding door and the bottom wall, the reset member being used to keep the sliding door in the closed state.

[0055] In the above technical solution, a reset component is set between the sliding door and the bottom wall. When the sliding door is opened, the reset component will accumulate elastic potential energy. When the sliding door loses the blocking effect of the moving component, the elastic potential energy can drive the sliding door to switch from the open state to the closed state under the action of the reset component, so that the sliding door can close automatically without the need for other drive mechanisms to drive and control the sliding door, making the structure simpler.

[0056] In some embodiments, the number of reset elements is set to two, and the two reset elements are distributed at intervals along the second direction.

[0057] In the above technical solution, by setting the number of reset components to two, the forces on both sides of the sliding door are more balanced under the elastic potential energy of the two reset components, making the sliding door slide more stably during opening or closing.

[0058] In some embodiments, the guide groove includes a first guide section and a second guide section. The first guide section is arranged in the same direction as the first direction to guide the reagent kit from the placement station to the product transfer station. The second guide section extends toward the bottom wall to guide the reagent kit into the collection bucket.

[0059] In the above technical solution, by including a first guide section and a second guide section in the guide groove, the first guide section can guide the reagent kit to move horizontally in the extraction channel, while the second guide section faces the bottom wall and can guide the reagent kit to slide out of the second guide section and flip downward, so that the reagent kit can detach from the hook part on the moving part and fall into the collection bucket after passing through the opening on the bottom wall under the action of gravity.

[0060] In some embodiments, the extraction channel is provided at the placement station with a leakage box for collecting leakage from the reagent kit.

[0061] In the above technical solution, since the reagent kit is located at the placement station of the extraction channel, samples, reagents or magnetic bead liquids are added to the corresponding chambers of the reagent kit. Therefore, during the addition process, there may be leakage, causing liquid to leak into the extraction channel. Therefore, by setting a leakage box at the placement station, the leakage box can collect the leakage of the reagent kit during the liquid addition process, thereby improving the cleanliness of the gene extraction module.

[0062] Secondly, this application also provides a gene detection device, which includes a frame, a worktable, a robotic arm, a first pipette, and the aforementioned gene extraction module. The worktable is horizontally positioned on the frame and includes a sample loading area and a nucleic acid extraction area. The sample loading area is used to place sample tubes, reagent kits, pipette tips, and reagents, and the nucleic acid extraction area is used for nucleic acid extraction. The robotic arm is mounted on the worktable and is used to transfer the reagent kit from the sample loading area to the nucleic acid extraction area. The first pipette is used to pipette the sample from the sample loading area to the nucleic acid extraction area. The nucleic acid extraction area is provided with at least one of the aforementioned gene extraction modules.

[0063] In the above technical solution, the gene detection equipment adopts a streamlined working mode. In the sample loading area, the samples, consumables and reagents required for nucleic acid extraction can be prepared and replenished. The robotic arm and the first pipette act as a transfer mechanism to transfer the samples, consumables and reagents to the nucleic acid extraction area. Then, one or more gene extraction modules can be set up in the nucleic acid extraction area, and multiple stations work in parallel, which improves the gene extraction efficiency accordingly.

[0064] In some embodiments, in the nucleic acid extraction area, the number of gene extraction modules is set to multiple, and the multiple gene extraction modules are arranged at intervals along the second direction in the nucleic acid extraction area.

[0065] In the above technical solution, by setting up multiple gene extraction modules in the nucleic acid extraction area and arranging them at intervals along the second direction on the worktable, each gene extraction module is equivalent to a nucleic acid extraction station, which enables the gene detection equipment to perform nucleic acid extraction at multiple stations simultaneously, greatly improving the nucleic acid extraction efficiency of the gene detection equipment.

[0066] In some embodiments, the sample loading area includes a sample tube area, a reagent kit area, a first pipette tip area, a first reagent area, and a first waste tip area. The sample tube area is used for storing and opening the sample tubes, the reagent kit area is used for storing the reagent kits, the first pipette tip area is used for storing the pipette tips, the first reagent area is used for storing the reagents, and the first waste tip area is used for storing the used pipette tips.

[0067] In the above technical solution, the sample loading area includes a sample tube area, a reagent kit area, a first pipette tip area, a first reagent area, and a first waste tip area. The reagent kit area can be used for sample loading or placing reagent kits. A robotic arm can then pick up the reagent kits from the reagent kit area and transfer them to the placement station in the nucleic acid extraction area, ready for the addition of reagents and samples. The sample tube area can be used to place or load sample tubes containing the nucleic acid samples to be tested. The sample tube area can also open the caps of the sample tubes, eliminating the need for manual opening or pre-opening, thus increasing the degree of automation. Then, the first pipette, using a pipette tip in the pick-up tip area, aspirates the sample from the opened sample tube and transfers it to the corresponding cavity of the reagent kit in the nucleic acid extraction area. After sample transfer, the first pipette discards the used pipette tip into the first waste tip area. The first reagent area stores the reagents required for the nucleic acid extraction process. The stored reagents can be aspirated by the first pipette tip and added to the corresponding reagent cavity of the reagent kit.

[0068] In some embodiments, the sample tube area includes a sampling station and a capping station. The sampling station is used to provide sample tubes, and the capping station is equipped with a sample dispensing module for opening the caps on the sample tubes.

[0069] In the above technical solution, the sample tube area includes a sampling station and a capping station. The sampling station provides sample tubes containing nucleic acid samples, while the sample dispensing module at the sampling station can open the caps on the sample tubes. This allows the system to support sample loading onto the original sample tubes without manual capping; the sample dispensing module automates the process, increasing automation, reducing the labor intensity of nucleic acid testing, and eliminating the risk of infection for personnel. Furthermore, separating the sampling station and the capping station allows for the temporary storage of more sample tubes at the sampling station, improving sample tube storage capacity. Of course, sample loading at the sampling station can be done manually or automatically using a loading device.

[0070] In some embodiments, the sample cup dispensing module includes a cup clamping mechanism and a capping robot mechanism. The cup clamping mechanism is located at the cap opening station and is used to clamp and fix the bottle body of the sample tube. The capping robot mechanism is located in the sample tube area and is used to clamp the cap of the sample tube and can rotate along its own axis to open the cap.

[0071] In the above technical solution, after the sample tube is sent to the capping station, the clamping cup mechanism first holds the body of the sample tube tightly, and then the capping robot clamps the cap of the sample tube and rotates it to open the cap. After the cap is successfully opened, the first pipette can pick up the tip and move it into the sample tube to aspirate the sample. The sample is then transferred to the corresponding chamber (lysis well) of the reagent kit at the nucleic acid extraction area placement station for subsequent extraction.

[0072] In some embodiments, the cup clamping mechanism includes a cup separating platform, a bottle body clamping assembly, and a clamping arm driving assembly. The cup separating platform has a positioning hole for placing the sample tube. The bottle body clamping assembly is disposed at the positioning hole and includes two clamping arms. The clamping arm driving assembly is drivenly connected to at least one of the two clamping arms, and the clamping arm driving assembly is used to drive the two clamping arms to move closer together to clamp the bottle body of the sample tube.

[0073] In the above technical solution, the positioning hole on the cup dispensing platform can hold the sample tube to be opened. Under the driving action of the clamping arm driving component, the two clamping arms drive one clamping arm to approach the other clamping arm, thereby clamping the body of the sample tube and completing the clamping and positioning of the sample tube, preventing the tube body from rotating together when the capping robot mechanism drives the tube cap to rotate.

[0074] In some embodiments, the capping robot mechanism includes a cap clamping assembly and a cap rotating assembly. The cap clamping assembly is used to clamp the cap of the sample tube. The cap rotating assembly is drivenly connected to the cap clamping assembly. When the cup clamping mechanism clamps the body of the sample tube, the cap rotating assembly can drive the cap of the sample tube to rotate relative to its own axis, so as to separate the cap of the sample tube from the body of the bottle.

[0075] In the above technical solution, the cap clamping mechanism in the capping robot can clamp the cap of the sample tube, and then the cap rotating component can be used to unscrew the cap of the sample tube, thus realizing automatic capping of the sample tube. This eliminates the need for manual capping, supports sample loading onto the original sample tube, and greatly reduces the workload of the staff.

[0076] In some embodiments, the sample dispensing module further includes a capping drive mechanism, which is drivenly connected to the capping robot mechanism so that the capping robot mechanism can move between the sampling station and the capping station to transfer the sample tube at the sampling station to the capping station for capping.

[0077] In the above technical solution, the capping robot can be moved by the capping drive mechanism, so that the capping robot can move to the sampling station and pick up the sample tube and transfer it to the capping station. In this way, there is no need to rely on other transfer mechanisms, such as robots. By using the capping drive mechanism in the sample cupping module in conjunction with the capping robot, the transfer of the sample tube can be achieved, making the sample cupping module more integrated and the structure more compact.

[0078] In some embodiments, the sample dispensing module further includes a sample barcode scanner, which is disposed on one side facing the capping robot mechanism, and is used to scan and read information from the identification code on the sample tube.

[0079] In the above technical solution, since the nucleic acid samples in the sample tubes are unique and different sample tubes contain different nucleic acid sample information, in order to facilitate the entry of the corresponding personnel information of the nucleic acid samples in the sample tubes, a sample barcode scanner is set in the sample dispensing module. After the sample tube is sent to the capping station, the capping robot of the sample dispensing module grabs the sample tube to the position of the sample barcode scanner, rotates it once to scan the barcode, thereby entering the information of the sample tube and realizing information-based testing.

[0080] In some embodiments, the reagent kit area is provided with a reagent kit loading module, which is used to transport the reagent kit to the reagent kit loading position, and the robotic arm is used to transfer the reagent kit located at the reagent kit loading position to the placement station of the nucleic acid extraction area.

[0081] In the above technical solution, by setting up a reagent kit feeding module on the workbench, the reagent kits can be automatically fed to the reagent kit area without the need for manual addition of reagent kits, reducing human intervention and minimizing human error. This achieves full automation of the nucleic acid extraction reagent kit feeding process. Once the reagent kit is fed to the reagent kit loading position, a robotic arm is only needed to transfer the reagent kit to the placement station in the nucleic acid extraction area, where it awaits nucleic acid extraction and use. The degree of automation is high.

[0082] In some embodiments, the workbench has a loading port communicating with the outside at the reagent loading position, and the reagent loading module is located below the workbench. The reagent loading module is used to load the reagent from bottom to top through the loading port to the reagent loading position.

[0083] In the above technical solution, by placing the reagent kit module below the workbench, the reagent kit can be fed from bottom to top through the loading port on the workbench into the reagent kit loading position. The reagent kit loading module is distributed vertically, which does not occupy the horizontal space of the gene detection equipment, making it easier to arrange the workbench surface and making the structure more compact.

[0084] In some embodiments, the reagent loading module includes a base, a reagent compartment, and a lifting assembly. The base is located below the workbench. The reagent compartment is disposed on the base and has a receiving cavity for storing reagents. The receiving cavity extends along a third direction and has a discharge port for discharging the reagents on a side near the loading port. The discharge port communicates with the loading port. The lifting assembly is disposed on the base and is used to lift the reagents in the receiving cavity along the third direction to the reagent loading position.

[0085] In the above technical solution, the reagent kits can be placed in the receiving cavity of the reagent kit compartment. Multiple reagent kits can be stacked in the reagent kit compartment. The lifting component can lift the reagent kits in the receiving cavity along a third direction to the reagent kit loading position, thereby realizing automatic reagent kit feeding without the need for manual reagent kit feeding, and the degree of automation is high.

[0086] In some embodiments, the lifting assembly includes a lifting section, a third lead screw, a third guide rail, and a third drive motor. The lifting section is used to lift the reagent kit. The third lead screw is distributed along the third direction, and the lifting section is threadedly engaged with the third lead screw. The third guide rail is disposed on the base and extends along the third direction. The third guide rail is slidably engaged with the lifting section to guide the lifting section when it moves. The third drive motor is disposed on the base and is drivenly connected to the third lead screw. The third drive motor is used to drive the third lead screw to rotate, thereby causing the lifting section to move within the receiving cavity along the third direction, so that the reagent kits in the reagent kit compartment are sequentially moved to the reagent kit loading position.

[0087] In the above technical solution, by setting the lifting component as a lead screw and nut pair mechanism, the movement of the lifting part is stable and reliable, and the third guide rail can guide the movement of the lifting part. The third drive motor is installed on the base as a driving force, which can drive the lifting part to move along the extension direction of the guide rail in the receiving cavity. Thus, the reagent kits in the reagent kit compartment can be continuously fed into the reagent kit loading position on the worktable through the loading port under the lifting action of the lifting part.

[0088] In some embodiments, a limiting component is provided at the loading port, the limiting component being used to support and limit the reagent kit located at the reagent kit loading position, so as to prevent the reagent kit located at the reagent kit loading position from falling into the reagent kit compartment.

[0089] In the above technical solution, by setting a limiting component at the loading port, the limiting component can provide auxiliary support for the reagent kit located at the reagent kit loading position, preventing the reagent kit at the reagent kit loading position from falling into the reagent kit compartment, thereby enabling the addition of reagent kits into the reagent kit compartment without stopping the machine.

[0090] In some embodiments, the reagent kit loading module further includes a sliding seat, which is slidably engaged with the base. The reagent kit compartment is mounted on the sliding seat, and the sliding seat has a third position and a fourth position on the base. When the sliding seat is in the third position, the discharge port of the reagent kit compartment communicates with the loading port. When the sliding seat is in the fourth position, the discharge port of the reagent kit compartment protrudes outside the workbench to facilitate the addition of reagent kits into the reagent kit compartment. The reagent kit compartment has a clearance hole on the side near the lifting part, which is used to make way when the reagent kit compartment is separated from the lifting part.

[0091] In the above technical solution, the reagent kit compartment is mounted on a sliding base. The sliding base and the base slide together, allowing the reagent kit compartment to slide outwards along the base and separate from the worktable, facilitating the replenishment or addition of reagent kits into the compartment. Specifically, when the sliding base is in the third position, the reagent kit compartment is connected to the loading port, allowing the reagent kits in the compartment to be normally supplied to the loading position. When the sliding base is moved from the third position to the fourth position, the discharge port of the reagent kit compartment is exposed outside the worktable, facilitating the replenishment of reagent kits into the compartment. Furthermore, the clearance holes on the side wall of the reagent kit compartment allow it to separate from the lifting section, ensuring that the feeding of reagent kits into the compartment is not affected.

[0092] In some embodiments, the reagent kit feeding module further includes a limit sensor, which is disposed on the third guide rail and is used to detect the operating status of the lifting section.

[0093] In the above technical solution, by setting a limit sensor on the third guide rail, the limit sensor can monitor the operating status of the lifting part, thereby controlling the stroke of the lifting part, ensuring the normal operation of the lifting part, and thus ensuring the normal feeding of reagent kits in the reagent kit compartment.

[0094] In some embodiments, the first pipette tip area is provided with at least one set of first pipette tip holders, the first pipette tip holders having a plurality of storage positions for storing the pipette tips, the pipette tips being used for pipetting with the first pipette; the first reagent area is provided with at least one set of reagent chambers, the reagent chambers being used to store reagents required for nucleic acid extraction.

[0095] In the above technical solution, because nucleic acid samples in the nucleic acid detection process are unique, to avoid cross-contamination between reagents and nucleic acid samples, the pipette tips used in the first pipette are all disposable. Therefore, the first pipette tip holder has multiple storage positions for placing pipette tips, making it convenient for the first pipette to retrieve and use the tips. Similarly, the nucleic acid extraction process requires the use of multiple reagents, so the first reagent area has multiple sets of reagent chambers, each corresponding to a reagent required for nucleic acid extraction, facilitating use during nucleic acid extraction.

[0096] In some embodiments, the gene detection device further includes a PCR system construction area, which is located on the workbench. The PCR system construction area and the sample loading area are located on opposite sides of the nucleic acid extraction area. The PCR system construction area includes a consumable area, a second reagent area, a second waste tip area, and a product placement area. The consumable area is used to place the consumables required for PCR system construction. The second reagent area is used to store the reagents required for PCR system construction. The second waste tip area is used to store the pipette tips used for PCR system construction. The product placement area is used to store the products after PCR system construction. The PCR system construction area is equipped with a second pipette, which is movably located in the PCR system construction area for transferring the products located in the product transfer area within the nucleic acid extraction area to the PCR system construction area for PCR system construction.

[0097] In the above technical solution, the nucleic acid extraction area spans the sample loading area and the PCR system construction area. The nucleic acid extraction kit enters the nucleic acid extraction device from the sample loading area and is passed all the way to the PCR system construction area. The second pipette in the PCR system construction area transfers the extracted product from the nucleic acid extraction area out of the kit and places it on the system construction rack for PCR system construction.

[0098] Other features and advantages of this application will be described in detail in the following detailed description section. Attached Figure Description

[0099] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly described below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0100] Figure 1 This is a schematic diagram of the structure of a gene detection device provided in some embodiments of this application;

[0101] Figure 2 This is a schematic diagram of the structure of a gene detection device provided in some embodiments of this application from another angle;

[0102] Figure 3 A top view of the workbench in a gene detection device provided in some embodiments of this application;

[0103] Figure 4 This is a schematic diagram of the sample tube area in a gene detection device provided in some embodiments of this application;

[0104] Figure 5This is a schematic diagram of the sample loading module in a gene detection device provided in some embodiments of this application;

[0105] Figure 6 for Figure 5 Schematic diagram of the structure of the upper sample base;

[0106] Figure 7 for Figure 5 Schematic diagram of the intermediate sample holder;

[0107] Figure 8 This is a schematic diagram of the sample dispensing module in a gene detection device provided in some embodiments of this application;

[0108] Figure 9 for Figure 8 Schematic diagram of the mechanism of the central cap rotating robot;

[0109] Figure 10 for Figure 8 A schematic diagram of the middle clamping cup mechanism;

[0110] Figure 11 This is a partial structural diagram of the sample loading area in a gene detection device provided in some embodiments of this application;

[0111] Figure 12 This is a schematic diagram of the structure of a reagent kit in a gene detection device provided in some embodiments of this application;

[0112] Figure 13 for Figure 12 Schematic diagram of the structure of the middle magnetic rod sleeve;

[0113] Figure 14 for Figure 12 Side sectional view;

[0114] Figure 15 This is a schematic diagram of the structure of the reagent kit feeding module in a gene detection device provided in some embodiments of this application;

[0115] Figure 16 for Figure 15 A schematic diagram of the structure of the lifting component;

[0116] Figure 17 This is a schematic diagram of the structure of the reagent kit loading module in a gene detection device provided in other embodiments of this application;

[0117] Figure 18 This is a schematic diagram of the structure of the robotic arm and pipette in a gene detection device provided in some embodiments of this application;

[0118] Figure 19 A schematic diagram showing the structure of the reagent kit located at the placement station in the gene extraction module of the gene detection device provided in some embodiments of this application;

[0119] Figure 20 A schematic diagram of the structure of the gene extraction module in the gene detection device provided in some embodiments of this application, showing the reagent kit located at the transfer station;

[0120] Figure 21 for Figure 19 A bottom view;

[0121] Figure 22 for Figure 21 Another structural diagram;

[0122] Figure 23 for Figure 20 A schematic diagram of the structure when the sliding door is open;

[0123] Figure 24 for Figure 23 Side sectional view;

[0124] Figure 25 A schematic diagram of the separation state of the reagent kit and the moving part in the gene extraction module provided in some embodiments of this application;

[0125] Figure 26 This is a schematic diagram of the heating element in the gene extraction module provided in some embodiments of this application;

[0126] Figure 27 This application provides schematic diagrams of the nucleic acid extraction mechanism in a gene extraction module according to some embodiments;

[0127] Figure 28 A schematic diagram of the structure of the hatch assembly of the gene extraction module provided in some embodiments of this application;

[0128] Figure 29 A schematic diagram of the grabbing unit of the gene extraction module provided in some embodiments of this application;

[0129] Figure 30 This is a schematic diagram of the waste liquid treatment system in the gene extraction module provided in some embodiments of this application;

[0130] Figure 31 This is a schematic diagram of the structure of a weighing sensor in a waste liquid treatment system provided in some embodiments of this application;

[0131] Figure 32 This is a schematic diagram of the structure of the system construction region in a gene detection device provided in some embodiments of this application.

[0132] Icons: 1000-Frame; 1001-Workbench; 100-Nucleic Acid Extraction Area; 101-Gene Extraction Module; 10-Base; 11-Extraction Channel; 12-Guide Groove; 20-Traction Mechanism; 21-Grip Unit; 210-Moving Component; 211-Hook; 2110-Locking Groove; 2111-Guide Inclined Surface; 212-Pushing Component; 23-First Drive Assembly; 230-First Drive Component; 231-Pulley; 24-Guide Rail; 30-Extraction Chamber; 32-Fixed Frame; 40-Nucleic Acid Extraction Mechanism; 41-Movable Frame; 420-Magnetic Rod Mounting Frame; 421-Magnetic Rod; 422-Second Drive Assembly; 430-Magnetic Rod Sleeve Mounting Frame; 4301-Socket; 431-Magnetic Rod Sleeve; 432-Third Drive Assembly Components; 50-Door assembly; 51-Door; 52-Lifting assembly; 520-Lifting motor; 521-Second guide rail; 61-Heating component; 610-Heating tank; 62-Cam; 70-Collection mechanism; 71-Collection bucket; 72-Sliding door; 73-Matching part; 74-Reset component; 80-Waste liquid treatment system; 81-Washing head; 82-Waste liquid storage container; 83-Cleaning agent storage container; 84-Fixing plate; 85-First horizontal drive mechanism; 86-First vertical drive mechanism; 87-First weighing sensor; 88-Second weighing sensor; 90-Leakage box; 200-Sample loading area; 201-First pipette tip area; 2010-First pipette tip holder; 2011-Pipette tip; 202-First waste head area; 2020-Waste pipette tip... Headrest; 20201 - Pipe tip clearance hole; 2021 - Pipe tip collection container; 203 - First reagent area; 204 - First reagent rack; 2040 - Partition; 205 - Second reagent rack; 300 - Sample loading module; 301 - Sample loading base; 3010 - Feed channel; 30101 - Inlet end; 3011 - Transfer channel; 3012 - Discharge channel; 30121 - Outlet end; 302 - First sample loading drive mechanism; 303 - Second sample loading drive mechanism; 304 - Third sample loading drive mechanism; 305 - Feed drawer; 306 - Discharge drawer; 307 - Detection component; 308 - Sample holder; 3081 - Sample tube; 400 - Sample dispensing module; 401 - Dispensing stage; 402 - Lateral opening drive mechanism; 402 0-Opening drive component; 4021-First opening pulley mechanism; 4022-Horizontal opening slide rail; 403-Vertical opening drive mechanism; 4030-Opening motor; 4031-First mounting base; 4032-First movable base; 404-Vertical opening drive mechanism; 405-Cup clamping mechanism; 4050-Positioning hole; 4051-Bottle body clamping assembly; 4052-Clamping arm drive assembly; 406-Capping robot mechanism; 4060-Bottle cap clamping assembly; 4061-Bottle capping assembly; 407-Sample barcode scanner; 500-Reagent kit; 501-Kit body; 5010-Disintegration hole; 5011-Magnetic rod sleeve hole; 5012-Washing hole; 5013-Eluting hole; 502-Magnetic rod sleeve; 5020-Matching section;5021-Transition section; 5022-Mixing section; 5023-Conical section; 503-Sealing boss; 504-Reinforcing rib; 505-Pick-up section; 506-Standing foot; 507-Graduation line; 508-Locking part; 600-Reagent kit loading module; 601-Base; 602-Reagent kit compartment; 6021-Discharge port; 6022-Leaning hole; 603-Lifting assembly; 6030-Lifting part; 6031 - Third lead screw; 6032 - Third guide rail; 6033 - Third drive motor; 605 - Third sliding seat; 700 - Robotic arm; 7001 - Mechanical gripper arm; 701 - First pipette; 702 - Three-axis drive mechanism; 7020 - Stand; 7021 - Second transverse drive mechanism; 70211 - First drive motor; 70212 - First slide rail; 7022 - Second longitudinal drive mechanism; 70221 - Longitudinal connecting frame; 70222 - Second drive motor; 70223 - Second slide rail; 7023 - Second vertical drive mechanism; 900 - PCR system construction area; 901 - Second pipette tip area; 902 - Second reagent area; 903 - Second waste tip area; 904 - Second pipette; 905 - Three-axis moving mechanism; 906 - System construction frame; X - First direction; Y - Second direction; Z - Third direction. Detailed Implementation

[0133] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0134] Unless otherwise defined, all technical and scientific terms used in this application have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains; the terminology used in the description of this application is for the purpose of describing particular embodiments only and is not intended to limit the application; the terms "comprising" and "having," and any variations thereof, in the description, claims, and accompanying drawings of this application are intended to cover non-exclusive inclusion. The terms "first," "second," etc., in the description, claims, or accompanying drawings of this application are used to distinguish different objects, not to describe a specific order or hierarchy.

[0135] In this application, the reference to "embodiment" means that a particular feature, structure, or characteristic described in connection with an embodiment may be included in at least one embodiment of this application. The appearance of this phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it a separate or alternative embodiment that is mutually exclusive with other embodiments.

[0136] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation," "connection," "linking," and "attachment" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0137] In this application, the term "and / or" is merely a description of the relationship between related objects, indicating that three relationships can exist. For example, A and / or B can represent: A existing alone, A and B existing simultaneously, or B existing alone. Additionally, in this application, the character " / " generally indicates that the preceding and following related objects have an "or" relationship.

[0138] In the embodiments of this application, the same reference numerals denote the same components, and for the sake of brevity, detailed descriptions of the same components are omitted in different embodiments. It should be understood that the thickness, length, width, and other dimensions of various components in the embodiments of this application shown in the accompanying drawings, as well as the overall thickness, length, width, and other dimensions of the integrated device, are merely illustrative and should not constitute any limitation on this application.

[0139] In this application, "multiple" means two or more (including two).

[0140] Genetic testing is a relatively precise detection method, primarily used in disease detection and material analysis. It is now known that human diseases are directly or indirectly related to genes. The detection and analysis of genes are increasingly widely used in areas such as hereditary diseases, tumors, and infectious diseases, leading to updates in disease diagnosis models and treatment strategies. Furthermore, it can reveal the pathogenesis of diseases related to abnormal gene structure or expression. Therefore, genetic testing has been widely applied in fields such as biomedicine. In practical applications, the main process of genetic testing includes sample loading and collection, sample gene processing (including sample lysis, washing, elution, PCR system construction, and PCR detection), thereby achieving gene detection of the sample.

[0141] The gene processing procedure is as follows: After lysing the sample using gene detection equipment, the genes in the sample are adsorbed by nano-magnetic beads. Then, the magnetic beads are washed with washing solution to remove residual lysate. Next, the nucleic acid (genes) on the magnetic beads are dissolved with elution solution. Finally, the nucleic acid (genes) products are transferred and a PCR system is constructed. The genes are then detected by PCR, thus completing the gene processing of the sample and outputting the detection results.

[0142] The inventors discovered that traditional gene analysis equipment is a completely new, fully automated nucleic acid detection and analysis instrument that integrates nucleic acid extraction, amplification, and detection analysis. This equipment allows for the storage and preparation of reagents and consumables, sample nucleic acid extraction, amplification, and result analysis. However, existing gene analysis equipment generally requires manual pre-opening of sample tubes, which increases the workload of operators and reduces sample loading efficiency. Furthermore, existing gene analysis equipment typically has only one nucleic acid extraction station, which can only extract one sample at a time, resulting in low nucleic acid extraction efficiency. It is also only suitable for small-volume sample testing and cannot handle large-volume samples. Therefore, existing gene analysis equipment suffers from low gene detection efficiency.

[0143] Based on the above considerations, in order to solve the problem of gene detection efficiency in gene detection equipment, the inventors, after in-depth research, designed a gene extraction module and a gene detection device that can improve the efficiency of gene detection.

[0144] According to some embodiments of this application, a gene detection device is provided. This device enables continuous nucleic acid extraction of genes, with the nucleic acid extraction zone configured as a cross-regional pipeline operation. Specifically, the nucleic acid extraction zone spans both the sample loading zone and the PCR system construction zone. Samples enter the nucleic acid extraction zone from the sample loading zone, and after nucleic acid extraction, they proceed to the PCR system construction zone. The nucleic acid extraction zone acts as a delivery module, effectively solving the contamination problem between different zones. Furthermore, the nucleic acid extraction zone employs multi-station gene extraction modules operating simultaneously in parallel, significantly improving nucleic acid extraction efficiency.

[0145] In some embodiments, please refer to Figure 1 , Figure 2 and Figure 3 The gene detection device includes a frame 1000, a workbench 1001, a robotic arm 700, and a first pipette 701. The workbench 1001 is horizontally positioned on the frame 1000 and includes a sample loading area 200, a nucleic acid extraction area 100, and a PCR system construction area 900. The sample loading area 200 is used to place sample tubes 3081, reagent kits 500, pipette tips 2011, and reagents. The nucleic acid extraction area 100 is used for nucleic acid extraction, and the PCR system construction area 900 is used for constructing a PCR system for the gene. The robotic arm 700 is mounted on the workbench 1001 and is used to transfer the reagent kit 500 from the sample loading area 200 to the nucleic acid extraction area 100. The first pipette 701 is used to pipette the sample from the sample loading area 200 to the nucleic acid extraction area 100. The nucleic acid extraction area 100 is equipped with at least one gene extraction module 101.

[0146] In this scheme, the gene detection equipment adopts a streamlined operation mode. Samples, consumables, and reagents required for nucleic acid extraction can be replenished in the sample loading area 200. The robotic arm 700 and the first pipette 701 act as transfer devices, moving the samples, consumables, and reagents to the nucleic acid extraction area 100. Multiple gene extraction modules 101 can be installed in the nucleic acid extraction area 100, and multi-station nucleic acid extraction is carried out in parallel, thereby improving gene extraction efficiency. The frame 1000 can be covered with an outer shell.

[0147] In addition, the gene detection device provided in this application embodiment also has the following additional technical features:

[0148] In some embodiments, please refer to Figure 3 The sample loading area 200 includes a sample tube area, a reagent kit area, a first pipette tip area 201, a first reagent area 203, and a first waste tip area 202. The sample tube area is used for storing and opening sample tubes 3081, the reagent kit area is used for storing reagent kits 500, the first pipette tip area is used for storing pipette tips 2011, the first reagent area 203 is used for storing reagents, and the first waste tip area 202 is used for storing used pipette tips 2011.

[0149] In the above technical solution, the sample tube area can be used to place sample tubes 3081 containing nucleic acid samples to be tested. The sample tube area can also open the caps of sample tubes 3081. Then, using a first pipette 701 and pipette tip 2011, the sample from sample tube 3081 is transferred to the corresponding reagent chamber of reagent kit 500 in the nucleic acid extraction area 100. After sample transfer, the first pipette 701 discards the used pipette tip 2011 into the first waste tip area 202. The reagent kit area can be used for sample loading or placing reagent kits 500. A robotic arm 700 can then pick up the reagent kits 500 from the reagent kit area and transfer them to the placement station in the nucleic acid extraction area 100, awaiting the addition of reagents and samples to the reagent kit 500. The first pipette tip area 201 is used to store spare pipette tips 2011, and the first reagent area 203 is used to store reagents required for the nucleic acid extraction process. The reagents are drawn by the first pipette 701 through pipette tip 2011 and added to the corresponding reagent chamber of reagent kit 500.

[0150] Please see Figure 4The sample tube area is used for loading and opening sample tubes 3081, facilitating the transfer of nucleic acid samples from sample tubes 3081 to the nucleic acid extraction area 100 by the robotic arm 700. The sample tube area may include a loading station and an opening station. The loading station is where sample tubes 3081 are loaded, and a loading module 300 may be installed there to automatically load samples into the sample tubes 3081. The opening station is where the caps of the sample tubes 3081 are opened. A sample dispensing module 400 may be installed at the opening station to open the caps on the sample tubes 3081.

[0151] The sample tube area includes a sample loading station and a capping station. The sample loading station provides sample tubes 3081, and the sample loading module 300 automatically loads the sample tubes 3081, saving manpower and making the gene detection equipment more automated. At the sampling station, the sample dispensing module 400 opens the caps on the sample tubes 3081, allowing the gene detection equipment to load the original sample tubes 3081 without manual capping. This automated capping by the sample dispensing module 400 further enhances automation, reduces the labor intensity of nucleic acid testing, and eliminates the risk of infection for personnel. Furthermore, separating the sampling and capping stations allows for temporary storage of more sample tubes 3081 at the sampling station, improving its storage capabilities. Of course, manual loading of sample tubes 3081 can still be performed at the sampling station.

[0152] In this embodiment, a sample loading module 300 is used at the sample loading station to automatically load the sample tube 3081. The sample loading module 300 can be a variety of devices, such as a conveyor belt type sample loading mechanism or a hook type drive traction sample loading mechanism.

[0153] In some embodiments, please refer to Figure 5 , Figure 6 and Figure 7 The sample loading module 300 is installed at the sample loading station of the workbench 1001. The sample loading module 300 may include a sample loading base 301, a first sample loading drive mechanism 302, a second sample loading drive mechanism 303, a third sample loading drive mechanism 304, and a detection component 307.

[0154] The sample base 301 has an inlet channel 3010, a transfer channel 3011, and an outlet channel 3012 for moving the sample holder 308. The sample holder 308 has multiple placement positions for placing sample tubes 3081. The inlet channel 3010 has an inlet end 30101 and a sampling station. A first sample loading drive mechanism 302 is connected to the sample base 301. The first sample loading drive mechanism 302 is used to drive the sample holder 308 from the inlet end 30101 along the inlet channel 3010 to the sampling station, so that the sample dispensing module 400 can remove the sample tubes 3081 from the placement positions and transfer them to the cap opening station for cap opening. The outlet channel 3012 is used for moving the sample holder 308 after sampling by the sampling module. The outlet channel 3012 has an outlet end 30121. The transfer channel 3011 connects the inlet channel 3010 and the outlet channel 3012. The second sample loading drive mechanism 303 is used to transfer the sample holder 308, after sampling by the sample dispensing module 400, from the sampling station along the transfer channel 3011 to the discharge channel 3012. The third drive mechanism is connected to the sample loading base 301, and the third sample loading drive mechanism 304 is used to drive the sample holder 308 transferred by the second sample loading drive mechanism 303 along the discharge channel 3012 to the outlet end 30121. The feed channel 3010 and the discharge channel 3012 are arranged side by side, and the extension direction of the transfer channel 3011 is perpendicular to the extension direction of the feed channel 3010 and the discharge channel 3012. The feed channel 3010, the transfer channel 3011, and the discharge channel 3012 together form a U-shaped channel on the base 10.

[0155] It should be noted that the discharge channel 3012 is a channel on the sample mounting base 301 used to guide the sample holder 308 after sampling by the sample dispensing module 400, so as to export the sample holder 308 from the outlet end 30121. The transfer channel 3011 is a channel on the sample mounting base 301 used to guide the sample holder 308 after sampling by the sample dispensing module 400, so as to transfer the sample holder 308 from the sampling station to the discharge channel 3012. The second drive mechanism is a mechanism that can drive the sample holder 308 after sampling by the sampling module to move in the transfer channel 3011. The third drive mechanism is a mechanism that can drive the sample holder 308 after sampling by the sampling module to move in the discharge channel 3012. The sample mounting base 301 includes a bottom wall for supporting the sample holder 308 and a side wall connected to the bottom wall, the bottom wall and the side wall forming the feed channel 3010, the transfer channel 3011 or the discharge channel 3012.

[0156] In some embodiments, please refer to Figure 6The first sample loading drive mechanism 302 may include a hook, a movable part, and a sample loading drive unit. The hook is disposed on the movable part, which is movably disposed on the side wall, and the sample loading drive unit is connected to the movable part. The hook is used to avoid the sample holder 308 when the sample loading drive unit drives the movable part to move in the reverse direction along the extension direction of the feed channel 3010. The hook is also used to hook onto the side of the sample holder 308 when the sample loading drive unit drives the movable part to move in the forward direction along the extension direction of the feed channel 3010, so as to drive the sample holder 308 from the inlet end 30101 along the feed channel 3010 to the sampling station. The sample loading drive unit may include a sample loading motor and a first lead screw. The sample loading motor is mounted on the bottom of the sample loading base 301, and the output end of the sample loading motor is connected to the first lead screw. The first lead screw is rotatably connected to the bottom of the sample loading base 301. The movable part is threadedly connected to the first lead screw. The bottom of the sample mounting base 301 is also provided with a first slide rail, which extends along the extension direction of the feed channel 3010. The movable part slides in cooperation with the first slide rail. When the sample mounting motor is activated, it drives the first lead screw to rotate. Since the movable part slides in cooperation with the first slide rail, the movable part cannot rotate. It can only move along the length direction of the first slide rail under the action of the first lead screw, thus realizing the movement of the movable part along the extension direction of the feed channel 3010.

[0157] In other embodiments, the sample loading drive unit may also include linear drive components such as linear electric cylinders, linear pneumatic cylinders, and linear hydraulic cylinders to directly drive the moving parts to move along the extension direction of the feed channel 3010.

[0158] It should be noted that the first sample loading drive mechanism 302, the second sample loading drive mechanism 303, and the third sample loading drive mechanism 304 can all be the same type of drive mechanism, differing only in their positions on the sample loading base 301. Specifically, the first sample loading drive mechanism 302, the second sample loading drive mechanism 303, and the third sample loading drive mechanism 304 are respectively located at the feeding channel 3010, the transfer channel 3011, or the discharge channel 3012. Therefore, the second sample loading drive mechanism 303 and the third sample loading drive mechanism 304 will not be described in detail here.

[0159] In some embodiments, the sample loading module 300 may further include an inlet drawer 305 and an outlet drawer 306. The inlet drawer 305 is movably disposed at the inlet end 30101 and is used to place the sample holder 308. The outlet drawer 306 is movably disposed at the outlet end 30121 and is used to receive the sample holder 308 driven by the third drive mechanism. The inlet drawer 305 and the outlet drawer 306 can be pulled out of the worktable 1001 to facilitate adding the sample holder 308 containing the sample tube 3081 into the inlet drawer 305. Similarly, the sample holder 308 can be removed from the outlet drawer 306 to facilitate the subsequent placement of the sample tube 3081 on the placement position of the sample holder 308, and then placed back into the inlet drawer 305 to realize the loading of the sample tube 3081.

[0160] By providing an infeed drawer 305, multiple sample holders 308 can be placed simultaneously before sampling. The first sample loading drive mechanism 302 automatically moves these sample holders 308 sequentially to the sampling station, awaiting the action of the sample dispensing module 400. This reduces the frequency with which operators place sample holders 308 before sampling by the sample dispensing module 400, thus reducing the workload of operators. Similarly, by providing an outlet drawer 306, sample holders 308 after sampling by the sample dispensing module 400 can be temporarily stored. Once the outlet drawer 306 is full of sample holders 308 after sampling by the sample dispensing module 400, the sample holders 308 in the outlet drawer 306 can be cleaned all at once, which helps reduce the frequency of cleaning the sample holders 308 and reduces the workload of operators.

[0161] The operator only needs to place the sample holder 308 at the inlet end 30101 of the feed channel 3010, and the first sample loading drive mechanism 302 will drive the sample holder 308 from the inlet end 30101 along the feed channel 3010 to the sampling station, thus facilitating the sample dispensing module 400 to retrieve samples from the sampling station (sample tube 3081). Since the sample holder 308 has multiple placement positions, but in actual analysis of biological samples, not every placement position is filled with a sample, often only some placement positions have samples.

[0162] Therefore, the sample loading module 300 may further include a detection component 307. Understandably, the detection component 307 is used to detect whether a sample tube 3081 is present at a placement position of the sample holder 308, and the sample dispensing module 400 responds to the detection result of the detection component 307. The detection component 307 is a detection element capable of detecting multiple placement positions within the sample holder 308 to confirm whether a sample is placed at each position. The detection component 307 can be linked with the sample dispensing module 400, causing the sample dispensing module 400 to move to the placement position with the sample tube 3081 and perform sampling based on the detection result of the detection component 307.

[0163] In this way, by setting the detection component 307, it can detect whether the placement position of the sample holder 308 has a sample tube 3081, determine which placement positions have a sample tube 3081 and which placement positions do not have a sample tube 3081, so that the sample dispensing module 400 can accurately move to the placement position with a sample tube 3081 when sampling, without empty movement, avoiding wasting time by the sample dispensing module 400, improving the sampling efficiency of the sample dispensing module 400, and improving the analysis efficiency of the biological sample tube 3081.

[0164] It should be noted that nucleic acid samples are generally placed inside sample tubes 3081. The placement positions on the sample holder 308 are designed to accommodate sample tubes 3081. Since sample tubes 3081 come in various sizes, the sample holder 308 also comes in multiple sizes, and the number and position of placement positions may differ for different sizes of sample holders 308. Please refer to [link / reference]. Figure 7 The length of the first sample holder 308 matches the width of the feed channel 3010. The first sample holder 308 is used to hold blood collection tubes. Because the diameter of the blood collection tubes is relatively small, the first sample holder 308 has a larger number of placement positions. The first sample holder 308 has two rows of placement positions, each row including five placement positions, for a total of ten placement positions. The second sample holder 308 has a shape and size roughly the same as the first sample holder 308, the difference being the number of placement positions. The second sample holder 308 is used to hold ordinary sample tubes 3081, which can hold tissue mixtures, etc. Because the diameter of the ordinary sample tubes 3081 is relatively large, the second sample holder 308 has fewer placement positions. The second type of sample holder 308 has two rows of placement positions, each row including four placement positions, for a total of eight placement positions.

[0165] It should be noted that the specifications of the sample holder 308 include, but are not limited to, the two mentioned above. That is, the sample holder 308 can also have more specifications to accommodate more types of sample tubes 3081, as long as the shape and size of the sample holder 308 can be limited by the feed channel 3010.

[0166] In some embodiments, to accommodate sample holders 308 of various sizes, the detection assembly 307 may include multiple rows of detection units arranged along the extension direction of the feed channel 3010. Each row of detection units is used to detect whether multiple placement positions within a sample holder 308 have sample tubes 3081. The detection units are components of the detection assembly 307 that implement the detection function. The detection assembly 307 includes multiple detection units arranged in rows, each row capable of detecting whether multiple placement positions within a sample holder 308 have samples. The detection units may be photoelectric sensors, and each placement position is provided with a detection hole capable of transmitting light signals emitted by the photoelectric sensor.

[0167] After the sample holder 308 moves to the sampling station position in the feeding channel 3010, the sample tube 3081 on the sample holder 308 at the sampling station is transferred to the opening station, and the sample cup module 400 starts to open the cup.

[0168] In some embodiments, please refer to Figure 8 , Figure 9 and Figure 10 The sample cup dispensing module 400 includes a cup clamping mechanism 405 and a capping robot mechanism 406. The cup clamping mechanism 405 is located at the cap opening station and is used to clamp and fix the bottle body of the sample tube 3081. The capping robot mechanism 406 is located in the sample tube 3081 area and is used to clamp the cap of the sample tube 3081 and can rotate along its own axis to open the cap. After the sample tube 3081 is delivered to the capping station, the clamping cup mechanism 405 first holds the body of the sample tube 3081 tightly, and then the capping robot mechanism 406 clamps the cap of the sample tube 3081 and rotates it to open the cap. After the capping is successfully opened, the first pipette 701 can pick up the pipette tip 2011 and move it into the sample tube 3081 to draw the sample. The sample is then transferred to the corresponding chamber (lysis well 5010) of the reagent kit 500 at the nucleic acid extraction area 100 placement station for subsequent extraction.

[0169] It should be noted that the sample tube 3081 located at the sampling station can be transferred to the cap-opening station in several ways. For example, the robotic arm 700 can be used to transfer the sample tube 3081 from the sampling station to the cap-opening station. Alternatively, the sample dispensing module 400 can be used to move itself to transfer the sample tube 3081 from the sampling station to the cap-opening station for opening.

[0170] Optionally, the sample dispensing module 400 may also include a cap-opening drive mechanism connected to the capping robot mechanism 406. The cap-opening drive mechanism adjusts the position of the capping robot mechanism 406 in space, allowing it to move between the sampling station and the cap-opening station. Therefore, by providing a cap-opening drive mechanism at the cap-opening station, the capping robot mechanism 406 can move from the cap-opening station to the sampling station, grabbing the sample tube 3081 located at the sampling station and transferring it to the cap-opening station for cap opening. This eliminates the need for other transfer mechanisms, such as the robot arm 700. By utilizing the cap-opening drive mechanism within the sample dispensing module 400 in conjunction with the capping robot mechanism 406, the transfer of the sample tube 3081 can be achieved, resulting in a higher integration and a more compact structure for the sample dispensing module 400.

[0171] Please refer to Figure 8 The cap-opening drive mechanism includes a cup-dispensing platform 401, a horizontal cap-opening drive mechanism 402, and a vertical cap-opening drive mechanism 403. The horizontal cap-opening drive mechanism 402 is connected to the cup-dispensing platform 401 and the vertical cap-opening drive mechanism 403, and can drive the vertical cap-opening drive mechanism 403 to move horizontally relative to the cup-dispensing platform 401, thereby enabling the vertical cap-opening drive mechanism 403 to move between the sampling station and the cap-opening station. The vertical cap-opening drive mechanism 403 is connected to a cap-screwing robot mechanism 406, and can drive the cap-screwing robot mechanism 406 to move vertically relative to the cup-dispensing platform 401. In this way, through the cooperation of the horizontal cap opening drive mechanism 402 and the vertical cap opening drive mechanism 403, the capping robot mechanism 406 can move in the horizontal and vertical directions, so that the capping robot mechanism 406 can move to the sampling station to take out the sample tube 3081 on the sample holder 308 and transfer it to the cap opening station for cap opening.

[0172] In some embodiments, the lateral lid-opening drive mechanism 402 may include a lid-opening drive member 4020 and a lid-opening pulley mechanism. The lid-opening drive member 4020 is connected to the drive wheel of the lid-opening pulley mechanism, and the vertical lid-opening drive mechanism 403 is connected to the transmission belt of the lid-opening pulley mechanism. When the lid-opening drive member 4020 is activated, it drives the drive wheel of the lid-opening pulley mechanism to rotate. The movement of the transmission belt is achieved through the cooperation of the drive wheel and the driven wheel of the pulley mechanism, thereby driving the vertical lid-opening drive mechanism 403 to move laterally relative to the cup-separating platform 401. To improve the stability of the vertical lid opening drive mechanism 403, a transverse lid opening slide rail 4022 extending laterally can be provided on the cup dispensing platform 401, so that the transverse lid opening slide rail 4022 and the vertical lid opening drive mechanism 403 slide together. The two ends of the guide rail 24 can be located at the lid opening station and the lid opening station respectively, thereby realizing the vertical lid opening drive mechanism 403 moving between the lid opening station and the lid opening station.

[0173] In some embodiments, the vertical cap-opening drive mechanism 403 includes a cap-opening motor 4030, a lead screw, a first movable seat 4032, a guide rail, and a first mounting base 4031. The first mounting base 4031 is connected to the horizontal cap-opening drive mechanism 402, and the guide rail is connected to the first mounting base 4031 and extends vertically. The first movable seat 4032 slides with the guide rail, allowing the first movable seat 4032 to be movably disposed on the first mounting base 4031 along the longitudinal direction. A cap-screwing robot mechanism 406 is connected to the first movable seat 4032. The cap-opening motor 4030 is mounted on the first mounting base 4031, and the output end of the cap-opening motor 4030 is connected to one end of the lead screw. The other end of the lead screw is rotatably disposed on the first mounting base 4031, and the first movable seat 4032 is threadedly connected to the lead screw. Under the action of the vertical capping drive mechanism 403, the capping robot 700 can move in the height direction, which makes it easier for the capping robot 700 to take out the sample tube 3081 at the sampling station. After moving to the top of the capping station under the drive of the horizontal capping drive mechanism 402, the sample tube 3081 can be placed on the cup clamping mechanism 405 at the capping station.

[0174] It should be noted that the implementation methods of the horizontal cap opening drive mechanism 402 and the vertical cap opening drive mechanism 403 are interchangeable. Furthermore, the horizontal cap opening drive mechanism 402 and the vertical cap opening drive mechanism 403 may also include linear drive components such as linear electric cylinders, linear pneumatic cylinders, and linear hydraulic cylinders to directly drive the cap-screwing robot mechanism 406 to achieve horizontal or vertical movement.

[0175] Furthermore, to facilitate the layout of the sample dispensing module 400, the sampling station and the capping station may not be on the same straight line in the longitudinal direction. Therefore, the capping drive mechanism can also include a longitudinal capping drive mechanism 404, which is used to realize the longitudinal movement of the capping robot mechanism 406. The specific structure of the longitudinal capping drive mechanism 404 can be referred to the transverse capping drive mechanism 402 and the vertical capping drive mechanism 403 mentioned above, and will not be repeated here. Thus, the capping robot mechanism 406 can realize movement along the transverse, longitudinal, and vertical directions, realizing three-axis movement of the capping robot mechanism 406 in space, thereby enabling the capping robot mechanism 406 to move freely and realize the capping of the sample tube 3081.

[0176] The number of first movable seats 4032 on the first mounting base 4031 can be set to one set, or two, three, or four sets, etc. When the number of sets of first movable seats 4032 is set to one, one capping robot mechanism 406 can transfer and open one sample tube 3081. Similarly, when the number of sets of first movable seats 4032 is set to two, two capping robot mechanisms 406 can be set on the first mounting base 4031. The two capping robots 700 can be set side by side, so that the two capping robot mechanisms 406 can simultaneously transfer and open two sample tubes 3081, thereby improving the capping efficiency of the sample tubes 3081.

[0177] In some embodiments, please refer to Figure 10 The cup clamping mechanism 405 includes a positioning hole 4050, a bottle body clamping assembly 4051, and a clamping arm driving assembly 4052. The positioning hole 4050 is disposed on the cup dispensing platform 401 and is used for placing the sample tube 3081. The bottle body clamping assembly 4051 is disposed at the positioning hole 4050 and includes two clamping arms. The clamping arm driving assembly 4052 is drivenly connected to at least one of the two clamping arms and is used to drive the two clamping arms to move closer together to clamp the bottle body of the sample tube 3081. The sample tube 3081 to be opened can be placed through the positioning hole 4050 on the cup dispensing platform 401. Under the driving action of the clamping arm drive assembly 4052, the two clamping arms drive one clamping arm to move closer to the other clamping arm, thereby clamping the tube body of the sample tube 3081 tightly, completing the clamping and positioning of the sample tube 3081, and preventing the tube body from rotating together when the capping robot arm mechanism 406 drives the tube cap of the sample tube 3081 to rotate.

[0178] When there are two sets of capping robot mechanisms 406, the number of positioning holes 4050 on the cup-separating platform 401 can also be set to two, and their arrangement direction and spacing can be adapted to the arrangement direction and spacing of the two capping robot mechanisms 406. In this way, under the action of the cap-opening drive mechanism, the capping robot mechanism 406 can simultaneously transfer the two sample tubes 3081 at the sampling station to the two positioning holes 4050 on the cup-separating platform 401, thereby using the bottle clamping components 4051 at the positioning holes 4050 to clamp the two sample tubes 3081 simultaneously. However, this is not a limitation; the number of positioning holes 4050 on the cup-separating platform 401 can also be three or four. The number of positioning holes 4050 can correspond to the number and position of the capping robot mechanisms 406.

[0179] In some embodiments, please refer to Figure 9 The capping robot mechanism 406 includes a cap clamping assembly 4060 and a cap rotating assembly. The cap clamping assembly 4060 clamps the cap of the sample tube 3081. The cap rotating assembly is driven to the cap clamping assembly 4060. When the cup clamping mechanism 405 clamps the body of the sample tube 3081, the cap rotating assembly can rotate the cap of the sample tube 3081 along its own axis to separate the cap from the body. By mounting the cap rotating assembly on the first movable seat 4032 and allowing it to move with the first movable seat 4032, the cap clamping mechanism in the capping robot mechanism 406 can clamp the cap of the sample tube 3081. Then, by using the rotational action of the cap rotating assembly, the cap of the sample tube 3081 is unscrewed, achieving automatic capping of the sample tube 3081. This eliminates the need for manual capping, supports sample loading onto the original sample tube 3081, and significantly reduces the workload of staff.

[0180] The bottle cap rotating assembly can be a variety of driving components. For example, the bottle cap rotating assembly can be a rotating motor, which is mounted on a movable seat, and the bottle cap clamping assembly 4060 is mounted on the driving end of the rotating motor, so that the bottle cap clamping assembly 4060 can rotate with the rotation of the driving end.

[0181] Alternatively, the bottle cap clamping assembly 4060 can be a variety of clamping mechanisms, such as clamping cylinders, which are located on both sides of the positioning hole 4050. Of course, the bottle cap clamping assembly 4060 can also be an electric gripper.

[0182] Optionally, an electric gripper can be used to clamp the cap of the sample tube 3081. The electric gripper has a simple structure and can clamp the cap of the sample tube 3081, making it easier to open the cap of the sample tube 3081 later.

[0183] In some embodiments, please refer to Figure 8The sample dispensing module 400 also includes a sample barcode scanner 407, which is positioned facing the capping robot mechanism 406. The sample barcode scanner 407 is used to scan the identification code on the sample tube 3081 to read information. Since the nucleic acid samples in the sample tube 3081 are unique, and different sample tubes 3081 contain different nucleic acid sample information, to facilitate the entry of the corresponding personnel information for the nucleic acid samples in the sample tube 3081, the sample dispensing module 400 includes a sample barcode scanner 407. After the sample tube 3081 is sent to the capping station, the capping robot mechanism 406 of the sample dispensing module 400 grasps the sample tube 3081 and moves it to the position of the sample barcode scanner 407, rotates it once to scan the code, thereby entering the information of the sample tube 3081 and realizing information-based testing.

[0184] The sample barcode scanner 407 can be installed on the horizontal cap opening drive mechanism 402, and the scanning direction of the sample barcode scanner 407 is set to one side facing the capping robot mechanism 406. During the process of the capping robot mechanism 406 transferring the sample tube 3081 at the sampling station to the cap opening station, the capping robot mechanism 406 can be rotated once to expose the barcode on the sample tube 3081, so that the sample barcode scanner 407 can scan the QR code of the sample tube 3081 and realize the information input and collection of the sample tube 3081.

[0185] In addition, after the sample tube 3081 is opened, the first pipette 701 can pick up the pipette tip 2011 and move it to the sample tube 3081 at the opening station, aspirating the sample from the sample tube 3081 and transferring it to the reagent kit 500 in the nucleic acid extraction area 100. After the sample transfer, the capping robot 700 screws the cap back onto the sample tube 3081. The cap clamping assembly 4060 releases the sample tube 3081, allowing the capping robot 700 to clamp the cap of the sample tube 3081 and move the sample tube 3081 back to the empty placement position on the sample holder 308 at the sampling station. Then, the capping robot mechanism 406 continues to repeat the previous action, transferring the sample tube 3081 on the sample holder 308 to the opening station. After all the samples in the sample holder 308 have been transferred, and all the sample holders 3081 on the sample holder 308 are empty sample holders 3081, the sample loading module 300 will then send the sample holder 308 out through the transfer channel 3011 and the discharge channel 3012 in sequence.

[0186] In some embodiments, please refer to Figure 11The sample loading area 200 includes a first pipette tip area 201, a first reagent area 203, and a first waste tip area 202. These need to be placed in advance before nucleic acid extraction. Before starting the first round of testing or before starting a new round of testing after completing the previous round of nucleic acid testing, general consumables (such as pipette tips 2011, reagents, and magnetic bead granules) should be placed on the workbench 1001.

[0187] In some embodiments, please refer to Figure 11 The first pipette tip area 201 is provided with at least one set of first pipette tip holders 2010. Each first pipette tip holder 2010 has several storage positions for storing pipette tips 2011, which are used by the first pipette 701 for pipetting. Due to the unique nature of samples during nucleic acid testing and to avoid cross-contamination between reagents and nucleic acid samples, all pipette tips 2011 used by the first pipette 701 are disposable. Therefore, the first pipette tip holder 2010 has multiple storage positions, which can accommodate multiple pipette tips 2011, facilitating the first pipette 701's retrieval and use of the pipette tips 2011.

[0188] The number and distribution of the first pipette tip holders 2010 within the first pipette tip area 201 can be flexibly configured. Similarly, the number of storage positions on the first pipette tip holders 2010 for placing the pipette tips 2011 can also be flexibly configured. These storage positions can be positioning structures such as positioning holes 4050 or positioning grooves on the first pipette tip holders 2010. These positioning structures can support the pipette tips 2011, ensuring they are placed vertically for accurate aspiration by the first pipette 701. Furthermore, the storage positions can only accommodate a portion of the pipette tips 2011, allowing the top of the pipette tips 2011 (i.e., the part that mates with the first pipette 701) to protrude beyond the first pipette tip holders 2010, facilitating the mating of the first pipette 701 with the pipette tips 2011.

[0189] In this embodiment, the number of first suction head holders 2010 in the first suction head area 201 can be set to five groups. Each first suction head holder 2010 has multiple storage positions, and the multiple storage positions are distributed in a rectangular array, a circular array, or an irregular distribution on the first suction head holder 2010.

[0190] Optionally, in this embodiment, the multiple storage positions on the first suction head holder 2010 are arranged in a rectangular array. The storage positions on the first suction head holder 2010 can be arranged in a 4×6, 5×6, 6×6, or 8×8 pattern, etc. The number and layout of the storage positions on the first suction head holder 2010 can be determined according to the actual situation. In this embodiment, the storage positions on the first suction head holder 2010 are distributed in a 4×6 pattern, and when there are five groups of first suction head holders 2010 in the first suction head area 201, then the first suction head area 201 can store 120 suction heads 2011.

[0191] Please refer to Figure 11 The first waste tip area 202 is used to collect the pipette tips 2011 after use by the first pipette 701. Please refer to [link / reference]. Figure 11 The first waste head area 202 is located on the workbench 1001, close to the first suction head area 201. The first waste head area 202 includes a waste suction head holder 2020 and a suction head collection bin 2021. The waste suction head holder 2020 has a suction head clearance hole 20201 for discarding suction heads 2011. The suction head collection bin 2021 is located below the suction head clearance hole 20201, allowing for the collection and storage of used suction heads 2011. While the suction head collection bin 2021 could be located between the workbench 1001 and the waste suction head holder 2020, this would result in a larger space occupied by the first waste head area 202 on the workbench 1001, affecting the overall layout. Therefore, the pipette tip collection container 2021 can also be located below the workbench 1001. A corresponding through-hole is provided on the workbench 1001 at the location of the clearance hole for the discarded pipette tip holder 2020, opposite to the pipette tip clearance hole 20201. This allows the used pipette tip 2011 to pass through the clearance hole 20201 and the through-hole into the pipette tip collection container 2021 located below the workbench 1001. By providing a first discarded pipette tip area 202 in the consumables area, the used pipette tip 2011 can be collected, preventing residual liquid from the used pipette tip 2011 from spilling onto the workbench 1001, thus avoiding cross-contamination between nucleic acids and affecting the accuracy of nucleic acid extraction. Using the pipette tip collection container 2021 to collect the used pipette tip 2011 ensures the overall cleanliness of the workbench 1001, thereby improving the accuracy of nucleic acid extraction results.

[0192] Specifically, when the first pipette 701 finishes using tip 2011 and needs to replace it, the first pipette 701 first moves to above the first waste tip area 202, then the first pipette 701 removes tip 2011. Due to gravity, tip 2011 falls into the waste tip holder 2020, passes through the clearance hole on the waste tip holder 2020, and falls into the waste container. Then, the first pipette 701 reinstalls a new tip 2011.

[0193] Since nucleic acid extraction requires the use of various reagents, these reagents may include one or more of the following: lysis buffer, magnetic bead solution, washing solution, elution buffer, and / or similar reagents. Therefore, one or more reagent racks are provided in the first reagent area 203. Each reagent rack can be used to store one or more reagents required for nucleic acid extraction, facilitating their use during extraction. The number of reagent racks in the first reagent area 203 can be one or more. The reagent racks can have various shapes, such as rectangular boxes, circular boxes, or triangular boxes. In this embodiment, the reagent rack is a rectangular box.

[0194] Please see Figure 11 The reagent racks can be divided into two models: the first reagent rack 204 and the second reagent rack 205. The first reagent rack 204 is used to store large-volume reagent solutions, and the second reagent rack 205 is used to store small-volume reagent solutions. For example, the first reagent rack 204 is used to store lysis buffer, washing buffer, and elution buffer, while the second reagent rack 205 is used to store small-volume reagents such as magnetic bead particle solutions.

[0195] When the reagent rack is a rectangular box, the rectangular box has a receiving cavity for holding reagent solutions. The top of the receiving cavity is open, and multiple partitions 2040 can be spaced apart in the receiving cavity. The multiple partitions 2040 can divide the receiving cavity into multiple chambers, each of which can be used to hold one type of reagent required for nucleic acid extraction. In this embodiment, four partitions 2040 are provided in the receiving cavity, and the four partitions 2040 can divide the receiving cavity into five chambers. That is, the rectangular box has five relatively independent chambers, which can hold up to five different reagent solutions. In the rectangular box, the top of each chamber is open, which facilitates the movement of the first pipette 701 to the top of the chamber and the use of the pipette tip 2011 to draw the reagent solution from the chamber. In this embodiment, the number of reagent racks in the first reagent area 203 is set to two, and the two reagent racks are arranged side by side.

[0196] Understandably, the kit is a carrier required for nucleic acid extraction. The kit can be used to place the nucleic acid sample to be tested and to extract nucleic acid from the sample.

[0197] In some embodiments, please refer to Figure 12 , Figure 13 and Figure 14The kit 500 includes a box body 501 and a magnetic rod sleeve 431. The box body 501 is provided with a magnetic rod sleeve hole 5011, a lysis hole 5010, a washing hole 5012, and an elution hole 5013. The magnetic rod sleeve hole 5011 is used to place the magnetic rod sleeve 431. The lysis hole 5010 is an elongated hole. The lysis hole 5010 allows the magnetic rod sleeve 431 to move along the length direction of the lysis hole 5010 after it has been inserted into the lysis hole 5010 along the depth direction.

[0198] The kit body 501 is the main structure of the kit 500 and the main load-bearing part of the kit 500. The magnetic rod sleeve hole 5011 is a hole formed in the kit body 501 for placing the magnetic rod sleeve 431. The lysis hole 5010 is a strip-shaped hole formed in the kit body 501 for placing lysis buffer, nucleic acid, magnetic beads, and for the magnetic rod sleeve 431 to extend into. The washing hole 5012 group includes at least one washing hole 5012, which is used to place washing buffer. The washing hole 5012 allows the magnetic rod sleeve 431 to extend into it, so that the magnetic rod sleeve 431 can reciprocate along the depth direction within the washing hole 5012 to clean the magnetic beads and remove impurities adsorbed by the magnetic beads. The elution well 5013 is used to hold the eluent. The elution well 5013 allows the magnetic rod sleeve 431 to extend into it, so that the magnetic rod sleeve 431 can move back and forth along the depth direction within the elution well 5013 to clean the magnetic beads and mix the nucleic acids adsorbed by the magnetic beads into the elution well 5013. The lysis wells 5010, the magnetic rod sleeve wells 5011, the washing wells 5012, and the elution wells 5013 can all be arranged along the length direction on the box body 501.

[0199] In some embodiments, the depths of the pyrolysis hole 5010, washing hole 5012, and elution hole 5013 on the box body 501 can all be the same, while the depth of the magnetic rod sleeve hole 5011 can be greater than the depth of the pyrolysis hole 5010, so as to facilitate the placement of the magnetic rod sleeve 431.

[0200] In some embodiments, please refer to Figure 13 The magnetic rod sleeve 431 includes a mating section 5020, a transition section 5021, a stirring section 5022, and a conical section 5023. The mating section 5020 allows the magnetic rod sleeve 431 to mate with the nucleic acid extraction mechanism 40, thus enabling the magnetic rod sleeve 431 to be installed on the nucleic acid extraction mechanism 40. The transition section 5021 includes a large end and a small end; the large end connects to the mating section 5020, and the diameter of the transition section 5021 gradually decreases from the large end to the small end. The stirring section 5022 is connected to the small end. The conical section 5023 is connected to the end of the stirring section 5022 furthest from the transition section 5021, and the conical section 5023 is conical in shape. The mating section 5020 is the largest diameter section on the magnetic rod sleeve 431, and it mates with the nucleic acid extraction mechanism 40 to achieve docking between the magnetic rod sleeve 431 and the nucleic acid extraction mechanism 40.

[0201] In some embodiments, a plurality of limiting portions are protruding on the inner or outer wall of the magnetic rod sleeve hole 5011. The plurality of limiting portions are arranged at intervals along the circumference of the magnetic rod sleeve hole 5011. The limiting portions are used to abut against the outer circumferential surface of the magnetic rod sleeve 431 when the magnetic rod sleeve 431 is placed in the magnetic rod sleeve hole 5011. By providing a plurality of limiting portions, it is convenient to abut against the outer circumferential surface of the magnetic rod sleeve 431 when the magnetic rod sleeve 431 is placed in the magnetic rod sleeve hole 5011, so as to limit the magnetic rod sleeve 431, avoid the magnetic rod sleeve 431 from being vibrated and colliding with the inner wall of the magnetic rod sleeve hole 5011, and prevent the magnetic rod sleeve 431 from shaking in the magnetic rod sleeve hole 5011. This is also conducive to the accurate docking of the nucleic acid extraction mechanism 40 and the magnetic rod sleeve 431.

[0202] In some embodiments, the kit 500 includes a sealing protrusion 503 disposed on the kit body 501, the sealing protrusion 503 being arranged around the opening of the magnetic rod sleeve hole 5011. By providing the third sealing protrusion 503, it is convenient to seal the magnetic rod sleeve hole 5011 when the magnetic rod sleeve 431 is installed inside the magnetic rod sleeve hole 5011, so as to prevent impurities from falling into the magnetic rod sleeve hole 5011 and contaminating the magnetic rod sleeve 431.

[0203] The number of magnetic rod sleeve holes 5011, pyrolysis holes 5010, washing holes 5012 and elution holes 5013 on the box body 501 can be one or more.

[0204] Optionally, if the number of magnetic rod sleeve holes 5011, lysis holes 5010, washing holes 5012 and elution holes 5013 on the box body 501 is one, then only one set of sample solution can be placed on the box body 501 for nucleic acid extraction.

[0205] Optionally, when there are multiple sets of magnetic rod sleeve holes 5011, lysis wells 5010, washing wells 5012, and elution wells 5013 on the kit body 501, these multiple sets of magnetic rod sleeve holes 5011, lysis wells 5010, washing wells 5012, and elution wells 5013 can all be arranged along the width direction of the lysis wells 5010. In this way, multiple samples can be placed on the kit body 501 simultaneously. Understandably, the kit 500 can simultaneously provide nucleic acid extraction for multiple sets of samples, thus enabling the extraction of multiple nucleic acid samples at once and improving the working efficiency of the nucleic acid extraction mechanism 40.

[0206] The number of magnetic rod sleeve holes 5011, pyrolysis holes 5010, washing holes 5012, and elution holes 5013 on the box body 501 can be any value, for example, six, eight, or nine. Combined with... Figure 1In the kit 500, the main body 501 includes eight magnetic rod sleeve holes 5011 arranged along the width direction, eight lysis holes 5010 arranged along the width direction, eight washing holes 5012 arranged along the width direction, and eight elution holes 5013 arranged along the width direction, which means that the kit 500 can simultaneously hold 8 samples for nucleic acid extraction.

[0207] Of course, the number of magnetic rod sleeve holes 5011, lysis holes 5010, washing holes 5012, and elution holes 5013 can be freely set as needed. For example, the kit 500 includes six magnetic rod sleeve holes 5011 arranged along the width direction, six lysis holes 5010 arranged along the width direction, six washing holes 5012 arranged along the width direction, and six elution holes 5013 arranged along the width direction. Alternatively, the kit 500 may include four magnetic rod sleeve holes 5011 arranged along the width direction, four lysis holes 5010 arranged along the width direction, four washing holes 5012 arranged along the width direction, and four elution holes 5013 arranged along the width direction.

[0208] In addition, to improve the cleaning effect of the washing wells 5012 on impurities adsorbed on the magnetic beads, each group of washing wells 5012 on the kit 500 can include 2 to 5 washing wells 5012, with multiple washing wells 5012 arranged along the length of the lysis wells 5010. By setting the number of washing wells 5012 in each group of washing wells 5012 to multiple, the nucleic acid extraction mechanism 40 can sequentially clean the magnetic beads with the magnetic rod sleeve 431 within the multiple washing wells 5012, ensuring the elution of impurities adsorbed on the magnetic beads and improving the purity of the nucleic acid adsorbed on the magnetic beads. For example, please refer to... Figure 1 Each set of washing holes 5012 consists of four washing holes 5012, which are arranged sequentially on the box body 501 along the length of the pyrolysis hole 5010.

[0209] In some embodiments, please refer to Figure 14 The bottoms of multiple washing units on the main body 501 are interconnected by reinforcing ribs 504. The bottom surface of the reinforcing ribs 504 forms a support surface. When the reagent kit 500 is placed on the workbench 1001, the uprights 506 and the support surface are in contact with the workbench 1001, jointly supporting the reagent kit 500. Alternatively, when multiple reagent kits 500 are stacked vertically, the adjacent reagent kits 500 can be supported by the reinforcing ribs 504, thereby improving the stability of the stacked reagent kits 500 and making them less likely to collapse.

[0210] In some embodiments, please refer to Figure 14The kit body 501 has a pickup section 505 formed on it for the robotic arm 700 to grasp. By providing the pickup section 505, the robotic arm 700 can easily pick up the kit body 501 by operating on the pickup section 505, thereby transferring the kit body 501 to different workstations, which helps improve the automation level of nucleic acid extraction. The pickup section 505 can have various structures; for example, it can be a top-down folded-edge structure formed on the top of the kit body 501. By forming a top-down folded-edge structure on the kit body 501 as the pickup section 505, the structure of the pickup section 505 is simple and convenient, easy to manufacture, and helps reduce the manufacturing cost of the kit body 501. In addition, the folded-edge structure also increases the strength of the kit 500.

[0211] Because the flanged structure surrounds the outer periphery of the box body 501, the robot arm 700 can grasp the box body 501 from all sides, with less restriction on the direction of the robot arm 700, making it more convenient for the robot arm 700 to grasp the box body 501. In some embodiments, the picking part 505 can be an annular protrusion protruding from the periphery of the box body 501.

[0212] In some embodiments, the kit body 501 also has a support foot 506 at the bottom of the lysis well 5010. When the kit 500 is placed on the workbench 1001, the support foot 506 contacts the workbench 1001 to support the kit 500. In addition, the outer surface of the lysis well 5010 is provided with a scale line 507, which can be used to determine the sample volume in the lysis well 5010.

[0213] In some embodiments, please refer to Figure 15 , Figure 16 and Figure 17 The reagent loading module 600 is located in the reagent area. This module loads reagent kits 500 and transports them to the reagent loading position on the workbench 1001. A robotic arm 700 then transfers the reagent kits 500 from the loading position to the placement station in the nucleic acid extraction area 100. By installing the reagent loading module 600 on the workbench 1001, the reagent kits 500 are automatically fed into the reagent area, eliminating the need for manual addition. This reduces human intervention and errors, achieving full automation of the nucleic acid extraction reagent kit loading process. Once the reagent kits 500 are loaded to the loading position, the robotic arm 700 simply transfers them to the placement station in the nucleic acid extraction area 100, awaiting nucleic acid extraction. This process demonstrates a high degree of automation.

[0214] In some embodiments, the workbench 1001 has a loading port communicating with the outside at the reagent loading position. The reagent loading module 600 is located below the workbench 1001. The reagent loading module 600 is used to load the reagent 500 from bottom to top through the loading port to the reagent loading position. By placing the reagent 500 module below the workbench 1001, the reagent 500 can be fed into the reagent loading position from bottom to top through the loading port on the workbench 1001. The reagent loading module 600 is vertically distributed and does not occupy the horizontal space of the gene detection equipment, which facilitates the reasonable arrangement of the workbench 1001 surface and makes the structure more compact.

[0215] In some embodiments, please refer to Figure 15 and Figure 16 The reagent kit loading module 600 includes a base 601, a reagent kit compartment 602, and a lifting component 603. The base 601 is located below the workbench 1001. The reagent kit compartment 602 is located on the base 601 and has a receiving cavity for storing reagent kits 500. The receiving cavity extends along a third direction (Z). The receiving cavity has a discharge port 6021 on the side near the loading port for discharging reagent kits 500, and the discharge port 6021 communicates with the loading port. The lifting component 603 is located on the base 601 and is used to lift the reagent kits 500 in the receiving cavity along a third direction (Z) to the reagent kit loading position. The receiving cavity in the reagent kit compartment 602 allows for the placement of reagent kits 500. Multiple reagent kits 500 can be stacked in the reagent kit compartment 602. The lifting component 603 can lift the reagent kits 500 in the receiving cavity along a third direction (Z) to the reagent kit loading position, thereby achieving automatic loading of reagent kits 500 without the need for manual loading, resulting in a high degree of automation.

[0216] The reagent kit compartment 602 can have various shapes, including a cuboid, cube, or cylinder. In this embodiment, the reagent kit compartment 602 is a cuboid. The reagent kit compartment 602 has a cavity for accommodating reagent kits 500, which allows for stacked placement of the reagent kits 500. The lifting component 603 applies an upward force to the top of the reagent kit compartment 602, pushing the stacked reagent kits 500 upwards to the reagent loading position of the loading port, facilitating the gripper 700 to grasp and transfer them to the nucleic acid extraction area 100. The diameter of the loading port on the worktable 1001 is adapted to the cross-section of the reagent kit 500, allowing the reagent kit 500 to enter the reagent loading position on the worktable 1001.

[0217] In some embodiments, please refer to Figure 16The lifting assembly 603 includes a lifting part 6030, a third lead screw 6031, a third guide rail 6032, and a third drive motor 6033. The lifting part 6030 is used to lift the reagent kit 500. The third lead screw 6031 is distributed along the third direction Z, and the lifting part 6030 is threadedly engaged with the third lead screw 6031. The third guide rail 6032 is disposed on the base 601 and extends along the third direction Z. The third guide rail 6032 is slidably engaged with the lifting part 6030 to guide the movement of the lifting part. The third drive motor 6033 is disposed on the base 601 and is drivenly connected to the third lead screw 6031. The third drive motor 6033 is used to drive the third lead screw 6031 to rotate, thereby driving the lifting part 6030 to move along the third direction Z within the receiving cavity, so that the reagent kits 500 in the reagent kit compartment 602 are sequentially moved to the reagent kit loading position.

[0218] In the above technical solution, by setting the lifting component 603 as a lead screw and nut pair mechanism, the movement of the lifting part 6030 is stable and reliable, and the third guide rail 6032 can guide the movement of the lifting part 6030. The third drive motor 6033 is installed on the base 601 as a driving force, which can drive the lifting part 6030 to move along the extension direction of the third guide rail 6032 in the receiving cavity. Thus, the reagent kit 500 in the reagent kit compartment 602 can be continuously fed into the reagent kit loading position on the worktable 1001 through the loading port under the lifting action of the lifting part 6030.

[0219] In some embodiments, a limiting component is provided at the loading port to support and limit the reagent kit 500 located at the reagent kit loading position, thereby preventing the reagent kit 500 located at the reagent kit loading position from falling into the reagent kit compartment 602. By providing a limiting component at the loading port, the limiting component can provide auxiliary support for the reagent kit 500 located at the reagent kit loading position, preventing the reagent kit 500 located at the reagent kit loading position from falling into the reagent kit compartment 602, thereby enabling the addition of reagent kit 500 into the reagent kit compartment 602 without stopping the machine.

[0220] The limiting component can have various structures. The limiting component is set at the loading port of the worktable 1001. The limiting component can be a spring sheet set on opposite sides of the loading port. As the lifting component 603 applies an upward force to the bottom of the stacked reagent kits 500, the reagent kits 500 will squeeze the spring sheets when passing through the loading port, causing the spring sheets to compress and deform to both sides of the loading port. When the reagent kits 500 move through the loading port to the reagent kit loading position, when the bottom of the reagent kits 500 exceeds the top of the spring sheets, the elastic potential energy accumulated by the spring sheets will be released. Thus, the spring sheets can support the bottom of the reagent kits 500 and prevent the reagent kits 500 from falling into the reagent kit compartment 602 below the loading port, so that they can be engaged and transported by the robot arm 700.

[0221] In some embodiments, please refer to Figure 17 The reagent loading module 600 also includes a third sliding seat 605, which is slidably engaged with the base 601. The reagent compartment 602 is mounted on the third sliding seat 605, which has a third position and a fourth position on the base 601. When the third sliding seat 605 is in the third position, the discharge port 6021 of the reagent compartment 602 communicates with the loading port. When the third sliding seat 605 is in the fourth position, the discharge port 6021 of the reagent compartment 602 protrudes outside the worktable 1001 to facilitate the addition of reagent kits 500 into the reagent compartment 602. The reagent compartment 602 has a clearance hole 6022 on the side near the lifting part 6030, which is used to make way when the reagent compartment 602 is separated from the lifting part 6030. By mounting the reagent kit compartment 602 on the third sliding seat 605, with the third sliding seat 605 slidingly engaged with the base 601, the reagent kit compartment 602 can slide outward along the base 601 and partially separate from the worktable 1001, facilitating the replenishment or addition of reagent kits 500 into the reagent kit compartment 602. Specifically, when the third sliding seat 605 is in the third position, the reagent kit compartment 602 is in communication with the loading port, and the reagent kits 500 in the reagent kit compartment 602 can be normally supplied to the reagent kit loading position. When the third sliding seat 605 is dragged from the third position to the fourth position, the discharge port 6021 of the reagent kit compartment 602 is exposed outside the worktable 1001, facilitating the replenishment of reagent kits 500 into the reagent kit compartment 602 through the discharge port 6021, thus realizing the addition of reagent kits 500 into the reagent kit compartment 602. Furthermore, the clearance hole 6022 provided on the side wall of the reagent kit compartment 602 allows the reagent kit compartment 602 to be separated from the lifting part 6030 without affecting the feeding of the reagent kit 500 inside the reagent kit compartment 602.

[0222] In some embodiments, the reagent kit loading module 600 further includes a limit sensor disposed on the third guide rail 6032 for detecting the operating status of the lifting section 6030. By providing a limit sensor on the third guide rail 6032, the limit sensor can monitor the operating status of the lifting section 6030, thereby controlling the stroke of the lifting section 6030, ensuring the normal operation of the lifting section 6030, and thus ensuring the normal loading of the reagent kits 500 into the reagent kit compartment 602.

[0223] Please see Figure 18Both the robotic arm 700 and the first pipette 701 can be movably mounted on the worktable 1001 and can move between the sample loading area 200 and the nucleic acid extraction area 100. In this way, the robotic arm 700 can pick up the reagent kit 500 at the loading position and transfer it to the nucleic acid extraction area 100, while the first pipette 701 can transfer the nucleic acid sample, magnetic beads, and reagents required for nucleic acid extraction located in the sample loading area 200 to the reagent kit 500 located in the nucleic acid extraction area 100, in preparation for nucleic acid extraction.

[0224] The workbench 1001 is equipped with a three-axis drive mechanism 702, which is connected to the first pipette 701 and the robot arm 700. The three-axis drive mechanism 702 is used to adjust the position of the first pipette 701 and the robot arm 700 in the space on the workbench 1001, that is, the spatial movement of part of the sample loading area 200 and the nucleic acid extraction area 100.

[0225] In some embodiments, the three-axis drive mechanism 702 includes a stand 7020, a second lateral drive mechanism 7021, a second longitudinal drive mechanism 7022, and a second vertical drive mechanism 7023. The stand 7020 is disposed on the worktable 1001 and extends along the distribution direction of the sample loading area 200 and the nucleic acid extraction area 100. The second lateral drive mechanism 7021 is connected to the stand 7020 and the second longitudinal drive mechanism 7022, the second vertical drive mechanism 7023 is connected to the second longitudinal drive mechanism 7022, and the robotic arm 700 and the first pipette 701 are mounted on the second vertical drive mechanism 7023. Furthermore, the second vertical drive mechanism 7022 may have one or two second vertical drive mechanisms 7023. When there are two second vertical drive mechanisms 7023, the first pipette 701 and the robotic arm 700 are respectively mounted on one of the two second vertical drive mechanisms 7023, so that the first pipette 701 and the robotic arm 700 can move relatively independently in the longitudinal and vertical directions.

[0226] Specifically, the second lateral drive mechanism 7021 can drive the second longitudinal drive mechanism 7022 to move laterally relative to the worktable 1001. Understandably, the lateral direction is the distribution and extension direction of the sample loading area 200 and the nucleic acid extraction area 100 on the worktable 1001. The second longitudinal drive mechanism 7022 is connected to two second vertical drive mechanisms 7023. The second longitudinal drive mechanism 7022 can drive the second vertical drive mechanisms 7023 to move longitudinally relative to the stand 7020. The first pipette 701 and / or the robotic arm 700 are mounted on the second vertical drive mechanism 7023, which can drive the first pipette 701 and / or the robotic arm 700 to move vertically relative to the stand 7020. Thus, through the cooperation of the second lateral drive mechanism 7021, the second longitudinal drive mechanism 7022, and the second vertical drive mechanism 7023, three-axis movement of the first pipette 701 and the robotic arm 700 within the space of the worktable 1001 can be achieved.

[0227] In some embodiments, the second lateral drive mechanism 7021 includes a first drive motor 70211, a first pulley mechanism, and a first slide rail 70212. Both the first drive motor 70211 and the first pulley mechanism are mounted on the upright 7020. The first drive member 230 is connected to the drive wheel of the first pulley mechanism. The longitudinal drive mechanism is connected to the transmission belt of the first pulley mechanism. The first slide rail 70212 is disposed on the upright 7020 and extends laterally. The second longitudinal drive mechanism 7022 is slidably engaged with the first slide rail 70212. The first slide rail 70212 can guide the second longitudinal drive mechanism 7022, thereby improving the stability of the second longitudinal drive mechanism 7022 during movement. When the first drive motor 70211 is activated, it drives the drive wheel of the first pulley mechanism to rotate. The movement of the transmission belt is achieved through the cooperation of the drive wheel and the driven wheel of the first pulley mechanism, thereby driving the second vertical moving mechanism to move laterally relative to the upright 7020 on the first slide rail 70212.

[0228] In some embodiments, the second longitudinal drive mechanism 7022 includes a longitudinal connecting frame 70221, a second drive motor 70222, a second pulley mechanism, and a second slide rail 70223. The longitudinal connecting frame 70221 has a sliding portion that slides with the first slide rail 70212. The second longitudinal drive mechanism 7022 is connected to the second transverse drive mechanism 7021 through the longitudinal connecting frame 70221. The second drive motor 70222 and the second pulley mechanism are both mounted on the longitudinal connecting frame 70221. The second drive motor 70222 is connected to the drive wheel of the second pulley mechanism. The second longitudinal drive mechanism 7022 is connected to the transmission belt of the second pulley mechanism. The second slide rail 70223 is disposed on the longitudinal connecting frame 70221 and extends longitudinally. The second vertical drive mechanism 7023 slides with the second slide rail 70223. The second slide rail 70223 can guide the vertical drive mechanism, thereby improving the stability of the second vertical drive mechanism 7023 when it moves. When the second drive motor 70222 is activated, it can drive the drive wheel of the second pulley mechanism to rotate. The drive wheel and driven wheel of the second pulley mechanism work together to realize the movement of the transmission belt, thereby driving the second vertical moving mechanism to move laterally on the second slide rail 70223 relative to the upright 7020.

[0229] In this design, the second vertical drive mechanism 7022 has two sets of second vertical drive mechanisms 7023, which are respectively connected to the first pipette 701 and the robotic arm 700. To ensure that the first pipette 701 and the robotic arm 700 can operate independently during longitudinal movement, the second drive component, the second pulley mechanism, and the second slide rail 70223 on the longitudinal connecting frame 70221 of the second vertical drive mechanism 7022 are all configured in two sets. Furthermore, the two sets of second drive motors 70222, second pulley mechanisms, and second slide rails 70223 can be vertically spaced on the longitudinal connecting frame 70221. The two second vertical drive mechanisms 7023 are respectively connected to any one of the two sets of second pulley mechanisms, and the two vertical drive mechanisms are slidably engaged with the corresponding second slide rails 70223. Although the first pipette 701 and the robot arm 700 share the same horizontal drive mechanism, the longitudinal and vertical movements of the first pipette 701 and the robot arm 700 can operate independently without interfering with each other.

[0230] In some embodiments, when two second vertical drive mechanisms 7023 are provided on the second longitudinal drive mechanism 7022, the two second vertical drive mechanisms 7023 have the same structure. The second vertical drive mechanism 7023 can be a lead screw and nut pair drive mechanism, which will not be described in detail here.

[0231] Alternatively, the robotic arm 700 can be an existing robotic arm 700 gripping mechanism. The robotic arm 700 includes a mounting plate, a gripping motor, and two mechanical gripping arms 7001. The mounting plate is mounted on a second vertical drive mechanism 7023, and the gripping motor is mounted on the mounting plate. The drive end of the gripping motor is connected to the two mechanical gripping arms 7001. The gripping motor can drive the two mechanical gripping arms 7001 to move closer and further apart, allowing them to cooperate and clamp the reagent kit 500 on opposite sides, thereby transferring the reagent kit 500. When the two mechanical gripping arms 7001 move further apart, they can separate from the reagent kit 500, allowing the reagent kit 500 to be placed down.

[0232] A compression spring component can also be provided between the two mechanical clamping arms 7001. The compression spring can provide elastic force when the two clamping arms are close to each other, thereby providing a buffering force when the two mechanical clamping arms clamp the reagent kit 500, and improving the clamping stability of the reagent kit 500.

[0233] In some embodiments, a pipette can also be provided on the movable seat of the robotic arm 700 installed in one of the two second vertical drive mechanisms 7023. That is, the robotic arm 700 and the pipette are simultaneously provided on the second vertical drive mechanism 7023, while a first pipette 701 is provided on the other second vertical drive mechanism 7023. The structure of the pipette 701 is exactly the same as that of the first pipette 701. Since the main function of the robotic arm 700 is to transfer the reagent kit 500 in the reagent kit 500 area to the nucleic acid extraction area 100, and the function of the first pipette 701 is to add the sample and reagent to the reagent kit 500 located in the nucleic acid extraction area 100, and since there are many types of reagents, multiple repetitions are required when adding reagents to the reagent kit 500. Moreover, in this embodiment, the reagent kit 500 is an eight-in-one consumable, and eight samples can be placed on the reagent kit 500 at the same time, so multiple repetitions are required. Therefore, in order to balance the working rationality of the two second vertical drive mechanisms 7023, a set of first pipettes 701 is set on the movable seat of the robot arm 700 mounted on the second vertical drive mechanism 7023. This is equivalent to both second vertical drive mechanisms 7023 being equipped with first pipettes 701. In this way, when the robot arm 700 is idle, the two can be used together, thereby avoiding the large amount of space occupied by the drive mechanism above the worktable 1001. Thus, by using a movable seat to achieve the sharing of the robot arm 700 and the first pipette 701, a dual-purpose machine is realized, which greatly saves the layout space of the worktable 1001.

[0234] Multiple pipettes can be mounted on the first pipette 701 on the second vertical drive mechanism 7023. It should be noted that the pipette can be any existing pipette, also known as a pipette gun, which is a device used for quantitative transfer of liquids. In analytical testing research, pipettes are generally used to transfer small or minute amounts of liquid. Based on their operating principle, pipettes can be divided into air-displacement pipettes and positive-displacement pipettes. Air-displacement pipettes are mainly used for standard pipetting, while positive-displacement pipettes are mainly used for handling volatile, corrosive, and viscous liquids.

[0235] In this embodiment, the number of pipettes mounted on the first pipette 701 on the second vertical drive mechanism 7023 can be one or more. The number of pipettes can be set according to the actual situation. When eight sets of nucleic acid samples can be placed on the reagent kit 500 at the same time, in order to improve the efficiency of reagent transfer by the first pipette 701, the number of pipettes can be set to two, four, or eight. The spacing between adjacent pipettes can match the spacing between adjacent lysis wells 5010, washing wells 5012, or elution wells 5013 on the reagent kit 500, so that multiple pipettes can simultaneously add reagents to multiple wells on the reagent kit 500, thereby greatly improving the reagent addition efficiency of the reagent kit 500. In this embodiment, the number of pipettes mounted on the first pipette 701 on the movable seat can be one.

[0236] In some embodiments, please combine Figure 1 In the nucleic acid extraction area 100, the number of gene extraction modules 101 is set to at least one. When multiple gene extraction modules are used, they are arranged at intervals along the second direction Y in the nucleic acid extraction area 100. By setting multiple gene extraction modules 101 in the nucleic acid extraction area 100 and arranging them at intervals along the second direction Y on the workbench 1001, each gene extraction module 101 is equivalent to a nucleic acid extraction station. This allows the gene detection equipment to perform nucleic acid extraction at multiple stations simultaneously, greatly improving the nucleic acid extraction efficiency.

[0237] Understandably, within the nucleic acid extraction area 100, the number of gene extraction modules 101 can be set to one, two, three, or four, etc.

[0238] For example, the number of gene extraction modules 101 is set to three. The three gene extraction modules 101 are arranged at intervals along the second direction Y in the nucleic acid extraction area 100, so that the gene detection device can perform nucleic acid extraction at three nucleic acid extraction stations at the same time, which greatly improves the nucleic acid extraction efficiency of the gene detection device.

[0239] In some embodiments, please refer to Figures 19-23 The gene extraction module 101 is located in the nucleic acid extraction area 100. The gene extraction module 101 includes a base 10, a traction mechanism 20, a nucleic acid extraction mechanism 40, and a collection mechanism 70. The base 10 includes an extraction channel 11 extending along a first direction X. The extraction channel 11 includes a placement station, an extraction station, a product transfer station, and a collection station in sequence along the first direction X. The placement station is used to place the reagent kit 500, the extraction station is used for nucleic acid extraction, and the product transfer station is used to transfer the extracted product. The traction mechanism 20 is located on the base 10 and is configured to pull the reagent kit 500 to move along the first direction X in the extraction channel 11, so that the reagent kit 500 moves from the placement station through the extraction station and the product transfer station to the collection station. The nucleic acid extraction mechanism 40 is located at the extraction station and is used to extract nucleic acids from the sample in the reagent kit 500. The collection mechanism 70 is located at the collection station and is used to collect the reagent kit 500 after the product transfer is completed.

[0240] In this scheme, the gene extraction module 101 adopts a streamlined nucleic acid extraction mode. The test kit 500 is placed at the placement station of the base 10, and the sample and reagents are added to the kit 500. Then, driven by the traction mechanism 20, the kit 500 can be transferred from the placement station to the extraction station for nucleic acid extraction. The extraction station is equipped with a nucleic acid extraction mechanism 40, which can perform normal nucleic acid extraction steps on the kit 500, such as lysis, washing and elution. After the nucleic acid separation in the kit 500 is completed, the kit 500 is moved from the extraction station to the product transfer station by the traction mechanism 20. At the product transfer station, the nucleic acid product in the kit 500 is transferred to the subsequent PCR system construction area 900. Then, under the action of the traction mechanism 20, the kit 500 is moved from the product transfer station to the collection station. The collection mechanism 70 at the collection station can collect the kit 500 after product transfer. Therefore, in this solution, the traction mechanism 20 set on the base 10 allows the reagent kit 500 to form a complete nucleic acid extraction process on the extraction channel 11 of the base 10, which greatly improves the continuity of nucleic acid extraction in the gene extraction module 101. That is, the series of operations from placing the reagent kit 500, adding samples, adding reagents, nucleic acid extraction, product transfer, and collecting the reagent kit 500 are all performed on the extraction channel 11 of the base 10, and are achieved by the traction action of the traction mechanism 20. Furthermore, when the previous reagent kit 500 is performing nucleic acid extraction at the extraction station, the next reagent kit 500 at the placement station is already ready to add samples. After the nucleic acid extraction of the previous reagent kit 500 is completed, the next reagent kit 500 located at the placement station can immediately enter the extraction station for nucleic acid extraction under the action of the traction mechanism 20. There is no interruption in the middle, the continuity is strong, and the extraction efficiency of nucleic acid extraction is improved.

[0241] In some embodiments, the extraction channel 11 includes two sidewalls opposite each other along a second direction Y, which is perpendicular to the first direction X. A guide groove 12 extending along the first direction X is provided on the sidewall, and the guide groove 12 guides the reagent kit 500 to move within the extraction channel 11. The guide groove 12, formed on the sidewall of the base 10, provides guidance and support for the movement of the reagent kit 500 within the extraction channel 11, thereby ensuring the stability and orientation of the reagent kit 500 during its movement within the extraction channel 11. This makes the nucleic acid extraction process of the reagent kit 500 within the extraction channel 11 more stable and reliable, thereby reducing the probability of reagent spillage during the movement of the reagent kit 500 within the extraction channel 11.

[0242] The guide groove 12 can have various structures. For example, the guide groove 12 can be a rib structure that protrudes from the side wall. The rib can provide support for both sides of the reagent kit 500, so that the reagent kit 500 can move on the guide groove 12. The guide groove 12 plays a guiding role for the reagent kit 500.

[0243] In some embodiments, please continue reading Figure 19 The traction mechanism 20 includes a gripping unit 21 and a first drive assembly 23. The gripping unit 21 is movably disposed on the base 10 along the first direction X and is used to grip and release the reagent kit 500. The first drive assembly 23 is disposed on the base 10 and is used to drive the gripping unit 21 to move along the first direction X on the base 10.

[0244] In the above technical solution, the grasping unit 21 can move the reagent kit 500 directionally on the extraction channel 11, thereby enabling the reagent kit 500 to complete nucleic acid extraction and product transfer processes sequentially.

[0245] In some embodiments, please refer to Figure 29 The gripping unit 21 includes a movable member 210, a claw portion 211, and an elastic member (not shown in the figure). The movable member 210 is movably disposed on the base 10 along a first direction X. The claw portion 211 is rotatably connected to the movable member 210. The claw portion 211 is provided with a guide slope 2111 and a locking groove 2110. The elastic member is disposed between the claw portion 211 and the movable member 210. The elastic member is used to apply an elastic force to the claw portion 211. When the movable member 210 moves to the point where the guide slope 2111 of the claw portion 211 contacts the locking portion 508 of the reagent kit 500, the locking portion 508 can squeeze the claw portion 211 and drive the claw portion 211 to rotate. The elastic member accumulates elastic potential energy. The elastic potential energy is used to drive the claw portion 211 to rotate and reset when the locking groove 2110 is engaged with the locking portion 508, so that the locking portion 508 is engaged with the locking groove 2110.

[0246] By rotating the gripper portion 211 in the gripping unit 21 with the moving member 210, when the moving member 210 moves to the point where the guide slope 2111 of the gripper portion 211 contacts the locking portion 508 on the reagent kit 500 located at the placement station, since the reagent kit 500 is stationary, the moving member 210 squeezes the reagent kit 500, that is, the gripper portion 211 squeezes the locking portion 508. The gripper portion 211 and the moving member 210 rotate in cooperation, and the guide slope 2111 on the gripper portion 211 can guide the gripper portion 211, thereby guiding the gripper portion 211 to rotate. At this time, the elastic element is compressed, thereby accumulating elastic potential energy, and the moving member 210 continues to squeeze. When the locking part 508 is engaged with the locking groove 2110 on the hook part 211, the elastic potential energy is released and acts on the hook part 211 to rotate and reset, thereby causing the locking part 508 to engage with the locking groove 2110. This achieves automatic squeezing and engaging between the hook part 211 on the moving part 210 and the locking part 508 on the reagent kit 500 without human intervention. It can automatically connect the gripping unit 21 and the reagent kit 500 with a high degree of automation. In this way, the gripping unit 21 can move with the reagent kit 500 in the placement station, extraction station, product transfer station and collection station in the extraction channel 11.

[0247] The elastic element can be a compression spring, a spring sheet, or a rubber column, etc. In this embodiment, a compression spring is used as the elastic element.

[0248] In some embodiments, please combine Figure 19 and Figure 20 The first driving assembly 23 includes a first driving member 230, a pulley 231, and a timing belt. The first driving member 230 is mounted on the base 10 and drives the pulley 231 to rotate. The timing belt is in transmission cooperation with the pulley 231 and extends along the first direction X. The moving member 210 is connected to the timing belt and moves along the first direction X on the base 10 under the action of the first driving member 230. By setting the first driving assembly 23 to a timing belt driving method, it occupies less space, has a more compact structure, and the timing belt driving method is stable and reliable. The first driving member 230 can be set on the outside of the extraction channel 11 on the base 10, and the timing belt can be set along the top of the base 10, so that the first driving assembly 23 does not occupy the internal space of the extraction channel 11, which facilitates the spatial arrangement of the nucleic acid extraction mechanism 40.

[0249] In order to improve the stability of the movement of the movable component 210 on the base 10, a guide rail 24 can be provided at the top of the extraction channel 11 of the base 10. The guide rail 24 can guide the movement of the movable component 210 and ensure the stability of the movable component 210 during its movement on the base 10.

[0250] In some embodiments, the base 10 is provided with an extraction chamber 30 at the extraction station, and the nucleic acid extraction mechanism 40 is disposed within the extraction chamber 30. The extraction chamber 30 is used to separate the placement station from the product transfer station. By providing the extraction chamber 30 on the base 10, the extraction chamber 30 can effectively isolate the placement station and the product station, making the interior of the extraction chamber 30 a relatively sealed environment, reducing the probability of gas leakage during nucleic acid extraction, thereby making the nucleic acid extraction process safer and more environmentally friendly. The extraction chamber 30 can be positioned above the extraction station on the extraction channel 11, and the nucleic acid extraction mechanism 40 can be installed on the extraction chamber 30, thereby facilitating the nucleic acid extraction mechanism 40 to extract nucleic acids from the reagent kit 500 below.

[0251] In some embodiments, the extraction chamber 30 has clearance openings formed between its two sides in the first direction X and the bottom wall of the extraction channel 11. These clearance openings allow the reagent kit 500 to enter or exit the extraction chamber 30. By providing clearance openings, it is easier for the reagent kit 500 to enter or exit the extraction chamber 30, thereby facilitating the corresponding nucleic acid extraction operation.

[0252] The extraction chamber 30 can be made of various materials. For example, it can be made of transparent material, which ensures the airtightness of the chamber while allowing staff to observe the nucleic acid extraction process from the outside. Of course, other materials can also be used for the extraction chamber 30, which will not be elaborated here.

[0253] It should be noted that a mounting bracket 32 ​​is installed inside the extraction chamber 30, and the mounting bracket 32 ​​is fixed to the base 10. The mounting bracket 32 ​​is used for the installation of the door assembly 50, the nucleic acid extraction mechanism 40, and other mechanisms.

[0254] In some embodiments, please combine Figure 20 and Figure 28 The extraction chamber 30 is equipped with a door assembly 50 at the avoidance opening, which is used to open and close the avoidance opening. Since some harmful gases are generated during the nucleic acid extraction process, these gases will escape from the avoidance opening to the outside of the extraction chamber 30, thus affecting the external air environment. Therefore, by providing the door assembly 50 at the avoidance opening, the avoidance opening can be closed and opened. Opening the avoidance opening facilitates the entry and exit of the reagent kit 500 into the extraction chamber 30. During the nucleic acid extraction process, the door assembly 50 is used to close the avoidance opening, thereby effectively reducing gas leakage generated during the nucleic acid extraction process, ensuring the airtightness of the extraction chamber 30 in the gene extraction module 101, and effectively ensuring the external air environment.

[0255] In some embodiments, please refer to Figure 28The door assembly 50 includes a door 51 and a lifting assembly 52. ​​The door 51 is used to close the clearance opening. The lifting assembly 52 is located in the extraction chamber 30 and is used to drive the door 51 to move along a third direction Z, which is perpendicular to the first direction X and the second direction Y. The lifting assembly 52 can drive the door 51 to move along the third direction Z on the extraction chamber 30. When it is necessary to open the clearance opening, the lifting assembly 52 drives the door 51 to move away from the bottom wall of the base 10, thus exposing the clearance opening and facilitating the entry or exit of the reagent kit 500 into the extraction chamber 30. When the reagent kit 500 enters the extraction chamber 30, the lifting assembly 52 drives the door 51 to move closer to the bottom wall, thereby closing the clearance opening. This prevents the leakage of gas generated during nucleic acid extraction in the extraction chamber 30, thus ensuring the external air environment of the gene extraction module 101.

[0256] The lifting assembly 52 can employ various drive mechanisms, such as linear cylinders, linear electric cylinders, linear hydraulic cylinders, synchronous belt drive mechanisms, or lead screw and nut pairs. In this embodiment, the lifting assembly 52 is a lead screw and nut pair mechanism, and the lifting assembly 52 is mounted on the fixed frame 32.

[0257] In some embodiments, please refer to Figure 28 The extraction chamber 30 is equipped with a second guide rail 521, and the door 51 has a corresponding slider on the side near the extraction chamber 30. The slider slides in engagement with the second guide rail 521. Through the sliding engagement between the second guide rail 521 and the slider on the door 51, the second guide rail 521 can guide the up and down movement of the door 51, thereby orienting the direction of movement of the door 51 and ensuring the stability of the door 51 during the up and down movement process.

[0258] The second guide rail 521 should be located on the same side of the extraction chamber 30 as the lifting assembly 52. ​​For example, the second guide rail 521 can be located outside the extraction chamber 30 or on the fixing frame 32 inside the extraction chamber 30. In this embodiment, the second guide rail 521 is located on the fixing frame 32 inside the extraction chamber 30, so that both the lifting assembly 52 and the second guide rail 521 are located inside the extraction chamber 30, which can improve the aesthetics of the extraction chamber 30. Furthermore, the door 51 can be provided with a flexible edge on the side near the bottom wall. The flexible edge can increase the contact area between the bottom of the door 51 and the bottom wall, improve the airtightness of the door 51, and also increase the buffering force when the door 51 contacts the bottom wall, avoiding rigid contact between the door 51 and the bottom wall.

[0259] In some embodiments, the extraction channel 11 is equipped with a sensing sensor on the side near the extraction chamber 30. This sensor is used to control the door assembly 50 when the moving part 210 enters or leaves the extraction station. By providing a sensing sensor on the side of the extraction channel 11 near the extraction chamber 30, the sensor can control the door assembly 50 based on the received signals, resulting in a higher degree of automation for the door assembly 50, eliminating the need for human intervention and enabling automatic control. Specifically, when the moving part 210 in the traction mechanism 20 approaches the extraction chamber 30, the sensing sensor detects the moving part 210 and sends a command to the control module. The control module then controls the door 51 to open, facilitating the passage of the moving part 210 through the extraction chamber 30.

[0260] The sensing sensor can be an infrared sensor, a photoelectric sensor, or other sensors. The sensing sensor is used to sense the moving part 210, thereby feeding the signal back to the control module, which controls the opening and closing of the hatch assembly 50, realizing the automatic opening and closing of the hatch 51 without the need for manual control, and has a high degree of automation.

[0261] In some embodiments, please refer to Figure 27 The nucleic acid extraction mechanism 40 includes a movable frame 41, a magnetic rod mounting frame 420, a magnetic rod sleeve mounting frame 430, a second drive assembly 422, a third drive assembly 432, and a fourth drive assembly. A fixed frame 32 is mounted on the base 10 and located within the extraction chamber 30. The movable frame 41 is movably mounted on the fixed frame 32 along a first direction X. The magnetic rod mounting frame 420 is movably mounted on the movable frame 41 along a third direction Z, and a magnetic rod 421 is mounted on the magnetic rod mounting frame 420. The third direction Z is perpendicular to the first direction X and the second direction Y. The magnetic rod sleeve mounting frame 430 is movably mounted on the movable frame 41 along the third direction Z. In the third direction Z, the magnetic rod sleeve mounting frame 430 is positioned relative to the magnetic rod mounting frame 420. Closer to the extraction channel 11; the second drive assembly 422 is disposed on the movable frame 41, and the second drive assembly 422 is used to drive the magnetic rod mounting frame 420 to move along the third direction Z; the third drive assembly 432 is disposed on the movable frame 41, and the third drive assembly 432 is used to drive the magnetic rod sleeve mounting frame 430 to move along the third direction Z; the fourth drive assembly is disposed on the fixed frame 32, and the fourth drive assembly is used to drive the movable frame 41 to move along the first direction X on the fixed frame 32; wherein, under the driving action of the second drive assembly 422 and the third drive assembly 432, the magnetic rod 421 can be inserted into the magnetic rod sleeve 431, and can be inserted into the reagent kit 500 located at the extraction station for magnetic attraction.

[0262] With the cooperation of magnetic rod 421 and magnetic rod sleeve 431, magnetic rod 421 can extend out of magnetic rod sleeve 431. Under the action of the second driving component 422 and the third driving component 432, magnetic rod sleeve 431 and magnetic rod 421 can be driven to move in the third direction Z and extend into the corresponding chamber of reagent kit 500. By inserting magnetic rod 421 into magnetic rod sleeve 431 and extending into the chamber of reagent kit 500 together, magnetic beads in reagent kit 500 can be adsorbed onto the outer surface of magnetic rod sleeve 431 to transfer magnetic beads. When magnetic rod 421 is withdrawn from magnetic rod sleeve 431, magnetic beads can be re-dissolved in lysis buffer or washing buffer. Therefore, by using magnetic rod sleeve 431 and magnetic rod 421 together, the transfer of nucleic acids attached to magnetic beads in each chamber of reagent kit 500 can be realized. Furthermore, through the sliding engagement of the fixed frame 32 and the movable frame 41, the movable frame 41, under the action of the fourth driving component, allows the magnetic rod 421, the fixed frame 32, and the magnetic rod sleeve mounting frame 430 to move along the first direction X within the extraction chamber 30. This allows the magnetic rod 421, in conjunction with the magnetic rod sleeve 431, to perform corresponding nucleic acid extraction operations on each reagent cavity of the reagent kit 500. By keeping the reagent kit 500 stationary within the extraction chamber 30 while the nucleic acid extraction mechanism 40 moves within it, the nucleic acid extraction operation on each reagent cavity of the reagent kit 500 is gradually achieved. This allows for a more compact internal space arrangement within the nucleic acid chamber, requiring only a space slightly larger than the length of the reagent kit 500 in the first direction X. This eliminates the need for the reagent kit 500 to move, resulting in a more rational internal space arrangement and a smaller volume within the extraction chamber 30.

[0263] Specifically, the magnetic nanobeads are pre-placed in the lysis chamber of the kit 500. The magnetic rod 421 is used to adsorb and capture the DNA nanobeads during the extraction process. After the extracted DNA is captured by the magnetic nanobeads, the second driving component 422 and the third driving component 432 operate, driving the magnetic rod 421 and the magnetic rod sleeve 431 to descend together and extend into the lysis well 5010 in the kit 500. Due to the strong magnetic force of the magnetic rod 421, all the magnetic nanobeads are adsorbed onto the outer surface of the magnetic rod sleeve 431. After a certain period of adsorption, the magnetic rod 421 and the magnetic rod sleeve 431 rise simultaneously, and then descend again to extend into the washing well 5012. Then, the magnetic rod 421 rises under the action of the second driving component 422. Since the magnetic rod 421 inside the magnetic rod sleeve 431 is removed, the magnetic nanobeads attached to the surface of the magnetic rod sleeve 431 will be re-dissolved in the washing solution, and the nucleic acids on the magnetic beads will be washed. After washing, when the magnetic beads are transferred to the next washing hole 5012, the magnetic rod 421 descends and is reinserted into the magnetic rod sleeve 431. The magnetic rod 421, inserted into the magnetic rod sleeve 431, descends into the washing hole 5012 to attract the magnetic beads, then rises together, and then moves horizontally and descends to another washing hole 5012, thereby realizing the transfer of the magnetic beads in each chamber of the reagent chamber.

[0264] In some embodiments, please continue reading Figure 27 The magnetic rod holder mounting bracket 430 is provided with an insertion hole 4301 for inserting the magnetic rod holder 431. Under the action of the third driving component 432, the magnetic rod holder mounting bracket 430 can extend into the magnetic rod holder 431 located in the reagent kit 500 for insertion, so that the magnetic rod holder 431 located in the reagent kit 500 is installed on the magnetic rod holder mounting bracket 430. Since the magnetic rod holder 431 is a disposable consumable and can only be used for the same sample, in the initial state, the magnetic rod holder 431 is located in the magnetic rod holder hole 5011 of the reagent kit 500. Then, when the reagent kit 500 enters the extraction chamber 30 under the action of the traction mechanism 20, the magnetic rod holder 431 is driven to descend under the action of the third driving component 432 and is installed on the magnetic rod holder 431 with the magnetic rod holder 431 inside the reagent kit 500, so as to cooperate with the magnetic rod 421 to complete the magnetic attraction of the magnetic bead. Therefore, the kit 500 comes with a magnetic wand sleeve 431, so that each sample can correspond to a magnetic wand sleeve 431, avoiding cross-contamination caused by the magnetic wand sleeve 431 combining with the reagent solution, and ensuring the accuracy of the results during the nucleic acid extraction process.

[0265] In some embodiments, the insertion hole 4301 extends in the Z-direction along the depth direction. The inner wall of the insertion hole 4301 is provided with a limiting groove, and the outer wall of the magnetic rod sleeve 431 is provided with a limiting protrusion that mates with the limiting groove, thereby restricting the axial movement of the magnetic rod sleeve 431 on the magnetic rod sleeve mounting bracket 430. By forming a limiting groove on the inner wall of the insertion hole 4301 and having a matching limiting protrusion on the outer wall of the magnetic rod sleeve 431, when the magnetic rod sleeve 431 bracket descends to be inserted into the magnetic rod sleeve 431 inside the reagent kit 500, the limiting protrusion on the outer wall of the magnetic rod sleeve 431 can engage with the limiting groove on the insertion hole 4301, thereby mounting the magnetic rod sleeve 431 on the magnetic rod 421 bracket and restricting the axial movement of the magnetic rod sleeve 431 on the magnetic rod sleeve mounting bracket 430. When it is necessary to remove the magnetic rod sleeve 431, simply insert the magnetic rod 421 into the magnetic rod sleeve 431 and push the magnetic rod sleeve 431 downwards to separate the magnetic rod sleeve 431 from the magnetic rod 421 frame, thereby achieving the disassembly of the magnetic rod sleeve 431, and the magnetic rod sleeve 431 is removed from the magnetic rod sleeve 431.

[0266] In some embodiments, the magnetic rod holder 4300 has multiple insertion holes 4301, which are arranged at intervals along the second direction Y. The magnetic rod holder 420 has multiple magnetic rods 421, with each magnetic rod 421 corresponding to the number and position of the insertion holes 4301. Since the reagent kit 500 can hold multiple sets of nucleic acid samples to be tested, by setting multiple insertion holes 4301 in the magnetic rod holder 430 within the nucleic acid extraction mechanism 40, corresponding to the number and position of the magnetic rods 421 on the magnetic rod holder 421, the nucleic acid extraction mechanism 40 can simultaneously extract nucleic acids from multiple samples in the reagent kit 500, greatly improving the extraction efficiency.

[0267] Optionally, if the reagent kit 500 is an eight-in-one consumable, it can hold eight sets of nucleic acid samples simultaneously. In this case, the number of insertion holes 4301 on the magnetic rod holder 430 is also set to eight, and the magnetic rod holder holes 5011 on the reagent kit 500 also have eight, with one magnetic rod holder 431 placed in each hole 5011. The distribution of insertion holes 4301 on the magnetic rod holder 431 corresponds to the distribution of magnetic rod holder holes 5011 on the reagent kit 500. Similarly, the magnetic rod holder 421 also has eight magnetic rods 421, and the distribution of the eight magnetic rods 421 corresponds to the distribution of insertion holes 4301 on the magnetic rod holder 431. However, this is not the only possibility. The reagent kit 500 can also be a six-in-one consumable. In this case, the reagent kit 500 can simultaneously hold six sets of nucleic acid samples. Therefore, the number of insertion holes 4301 on the magnetic rod holder 430 is also set to six, and the magnetic rod holder holes 5011 on the reagent kit 500 also have six, with one magnetic rod holder 431 placed in each hole 5011. The distribution of insertion holes 4301 on the magnetic rod holder 431 corresponds to the distribution of magnetic rod holder holes 5011 on the reagent kit 500. Similarly, the magnetic rod holder 421 also has eight magnetic rods 421, and the distribution of the six magnetic rods 421 corresponds to the distribution of insertion holes 4301 on the magnetic rod holder 431.

[0268] In some embodiments, the nucleic acid extraction mechanism 40 further includes a magnetic rod sleeve detection component, which is disposed on the movable frame 41 and is used to detect the presence of the magnetic rod sleeve 431 on the magnetic rod sleeve mounting frame 430. Since the magnetic rod sleeve 431 on the magnetic rod sleeve mounting frame 430 is automatically installed, by providing the magnetic rod sleeve detection component on the movable frame 41, it is possible to detect whether the magnetic rod sleeve 431 is installed on the magnetic rod sleeve mounting frame 430. If the magnetic rod sleeve 431 is not successfully installed on the magnetic rod sleeve mounting frame 430, it will affect the subsequent nucleic acid extraction operation. Therefore, the detection is performed by the magnetic rod sleeve detection component to ensure that the magnetic rod sleeve 431 on the magnetic rod sleeve mounting frame 430 can be used for nucleic acid extraction after installation is completed.

[0269] When moving the lysis well 5010 on the reagent kit 500 along its length (i.e., the first direction X), the traction mechanism 20 can drag the reagent kit 500 within the extraction chamber 30 while the nucleic acid extraction mechanism 40 remains stationary. Alternatively, the traction mechanism 20 can remain stationary within the extraction chamber 30 while the nucleic acid extraction mechanism 40 moves within the extraction chamber 30.

[0270] In some embodiments, please refer to Figure 25 and Figure 26 The gene extraction module 101 also includes a heating element 60, which is located at the extraction station and is used to heat at least a portion of the bottom of the reagent kit 500. Since both the lysis and elution reactions during nucleic acid extraction require heating to promote them, the heating element 60 at the extraction station heats the corresponding portions of the bottom of the reagent kit 500 (i.e., the lysis wells 5010 and elution wells 5013), thereby ensuring the normal progress of nucleic acid extraction within the reagent kit 500.

[0271] In some embodiments, the heating element 60 is movably disposed within the extraction channel 11 along a third direction Z, which is perpendicular to the first direction X and the second direction Y. To ensure effective heating of the bottom of the reagent kit 500 by the heating element 60 and rapid heat dissipation after heating, the heating element 60 is positioned both close to and away from the reagent kit 500. When heating of the bottom of the reagent kit 500 is required, the heating element 60 is brought close to and in contact with the reagent kit 500 to heat the corresponding portion of the reagent kit 500. After heating the bottom of the reagent kit 500, the heating element 60 is moved away from the reagent kit 500 to allow for rapid heat dissipation from the bottom of the reagent kit 500. Compared to automatic cooling by the heating element 60, moving the heating element 60 away from the reagent kit 500 avoids residual heat from the heating element 60 negatively impacting the nucleic acid sample within the reagent kit 500 chamber, thus improving the accuracy of nucleic acid extraction.

[0272] In some embodiments, the heating element 60 has a first position contacting the bottom of the reagent kit 500 and a second position separated from the bottom of the reagent kit 500. The gene extraction module 101 further includes a fifth driving component mounted on a side wall. The fifth driving component is used to drive the heating element 60 to switch between the first and second positions. By providing the fifth driving component, the heating element 60 can be driven to move between the first and second positions. When the reagent kit 500 needs to be heated, the fifth driving component drives the heating element 60 from the second position to the first position, so that the heating element 60 contacts the bottom of the reagent kit 500 and heats the corresponding chamber of the reagent kit 500. After the corresponding chamber of the reagent kit 500 has been heated, the fifth driving component drives the heating element 60 to switch from the first position to the second position, so that the heating element 60 can be separated from the reagent kit 500, and the heating of the reagent kit 500 can be stopped. Therefore, by providing the fifth driving component, the heating element 60 can be moved closer to or further away from the reagent kit 500, realizing automatic heating of the reagent kit 500 without the need for manual operation of the heating element 60, and achieving a higher degree of automation.

[0273] The fifth drive component can be a variety of drive mechanisms, such as cylinders, hydraulic cylinders, lead screw and nut pairs, or cam mechanisms, all of which can enable the heating element 60 to move between the first position and the second position.

[0274] Optionally, please refer to Figure 25 The fifth drive assembly includes a cam 62 and a fifth drive member. The cam 62 contacts the bottom of the heating element 60. The fifth drive member is mounted on the side wall and is used to drive the cam 62 to rotate, so that the heating element 60 switches between a first position and a second position. By adopting a cam 62 mechanism for the fifth drive assembly, the cam 62 is driven to rotate under the action of the fifth drive member. The cam 62 is always in contact with the bottom of the heating element 60. Under the rotation of the cam 62, the heating element 60 can switch between a first position and a second position. When the heating element 60 is in the first position, the heating element 60 contacts the bottom of the reagent kit 500, thereby heating the corresponding chamber at the bottom of the reagent kit 500. When the cam 62 continues to rotate until the heating element 60 no longer contacts the reagent kit 500, the heating element 60 stops heating the reagent kit 500.

[0275] In some embodiments, please refer to Figure 26 The heating element 60 has a plate-like structure and includes a bottom wall with protruding heating grooves 610. The heating grooves 610 are designed to contact the bottom of the reagent kit 500 and can accommodate at least a portion of the bottom of the reagent kit 500 in the third direction Z. The position and number of the heating grooves 610 on the heating element correspond one-to-one with the position and number of the lysis wells 5010 and elution wells 5013 in the reagent kit 500.

[0276] By forming a heating groove 610 on the bottom wall of the heating element 60, with the depth direction of the heating groove 610 aligned with the depth direction of the orifice in the reagent kit 500, the heating groove 610 can increase the contact area between the heating element 60 and the corresponding outer wall of the cavity in the reagent kit 500, thereby improving the heating effect on the bottom of the reagent kit 500, shortening the heating time, and correspondingly improving the efficiency of nucleic acid extraction.

[0277] The heating element 60 can be installed inside the extraction channel 11 or below the bottom wall of the base.

[0278] Optionally, when the heating element 60 is placed in the extraction channel 11, the fifth driving element is installed on the side wall, and the side wall is also provided with a guide groove for the heating element 60 to move up and down. The guide groove can guide the up and down movement of the heating element 60, and the cam 62 is rotatably set on the side wall.

[0279] Optionally, when the heating element 60 is positioned below the bottom wall of the extraction channel 11, a guide hole can be provided on the bottom wall for the heating groove 610 on the heating element 60 to extend out of the bottom wall. The guide hole can guide the up-and-down movement of the heating element 60. When the heating element 60 is in the first position, the heating groove 610 on the heating element 60 extends at least partially out of the guide hole, that is, into the extraction channel 11. The heating groove 610 of the heating element 60 extending into the extraction channel 11 contacts the corresponding chamber at the bottom of the reagent kit 500, thereby heating the bottom of the reagent kit 500. When the heating element 60 is in the second position, the heating groove 610 on the heating element 60 is located inside the guide hole. The heating groove 610 seals the guide hole, thereby ensuring the sealing of the bottom wall and preventing external air below the base 10 from entering the extraction channel 11.

[0280] In some embodiments, please refer to Figures 22-25 and combined Figure 1The collection mechanism 70 includes a collection bucket 71 located below the collection station. The collection bucket 71 is used to collect the reagent kit 500 after product transfer. One end of the guide groove 12 extends between the placement station and the extraction station, and the other end extends between the product transfer station and the collection station. When the reagent kit 500 moves to the collection station, it detaches from the guide groove 12 and falls into the collection bucket 71 under gravity. The guide groove 12 disconnects at the collection station. When the reagent kit 500, after product transfer, is about to enter the collection station, the gripping unit 21 pulls the reagent kit 500 detach from the guide groove 12. After one side of the reagent kit 500 loses the support of the guide groove 12, it detaches from the guide groove 12 and falls into the collection bucket 71 under gravity, thus automatically completing the collection of the reagent kit 500. Therefore, the gene extraction module 101 can complete the entire production line operation of sample addition, extraction, transfer and collection of reagent kit 500 without the need to use other transfer auxiliary mechanisms such as robotic arm 700 to transfer reagent kit 500 to other workstations, making the structure integration of gene extraction module 101 higher.

[0281] In some embodiments, the base 10 has a collection cavity communicating with the outside below its bottom wall, a collection bucket 71 is located inside the collection cavity, and the bottom wall has an opening at the collection cavity communicating with the extraction channel 11; the collection mechanism 70 also includes a sliding door assembly, which is movably disposed at the opening and is used to close and open the opening. By providing an opening in the bottom wall of the base 601, placing the collection bucket 71 below the opening, and then using the sliding door assembly to close and open the opening, the airtightness of the extraction channel 11 inside the base 10 can be ensured.

[0282] In some embodiments, the sliding door assembly includes a sliding door 72 and a mating part 73. The sliding door 72 is slidably disposed at the opening along a first direction X. A groove extending along the first direction X is provided on the side wall near the bottom wall. The groove slidably engages with the sliding door 72. The sliding door 72 has a closed state and an open state relative to the opening. The mating part 73 is disposed on the sliding door 72. A pushing part 212 that engages with the mating part 73 is provided on the moving member 210. Under the movement action of the moving member 210, the moving member 210 pushes the mating part 73 through the pushing part 212, and drives the sliding door 72 to slide along the first direction X, so that the sliding door 72 switches from the closed state to the open state.

[0283] By providing a mating part 73 on the sliding door 72 and a pushing part 212 on the moving part 210, during the process of the moving part 210 moving from the product transfer station to the collection station, the pushing part 212 on the moving part 210 can cooperate with the mating part 73. The moving part 210 will push the mating part 73 and drive the sliding door 72 to move, causing the opening to open automatically. At this time, the reagent kit 500 slides out of the guide groove 12. After the reagent kit 500 separates from the hook part 211 on the gripping unit 21, the reagent kit 500 will fall into the collection bucket 71 from the opening under the action of gravity. Therefore, in this solution, the sliding door 72 can be automatically opened by the linkage action of the moving part 210 on the traction mechanism 20, without the need for other drive structures or control mechanisms to open the sliding door 72, making the structure simpler and more compact.

[0284] In some embodiments, a reset member 74 is further provided between the sliding door 72 and the bottom wall. The reset member 74 is used to maintain the sliding door 72 in the closed state. Through the reset member 74 provided between the sliding door 72 and the bottom wall, when the sliding door 72 is opened, the reset member 74 accumulates elastic potential energy. When the sliding door 72 loses the obstruction effect of the moving member 210, the elastic potential energy of the reset member 74 can drive the sliding door 72 to switch from the open state to the closed state, allowing the sliding door 72 to close automatically without the need for other drive mechanisms to drive and control the sliding door 72, resulting in a simpler structure.

[0285] In some embodiments, the number of reset members 74 is set to two, and the two reset members 74 are distributed at intervals along the second direction Y. By setting the number of reset members 74 to two, the forces on both sides of the sliding door 72 under the action of the elastic potential energy of the two reset members 74 are more balanced, making the sliding door 72 slide more stably during opening or closing.

[0286] In some embodiments, the guide groove 12 includes a first guide section and a second guide section. The first guide section is arranged in the same direction as the first direction X and is used to guide the reagent kit 500 from the placement station to the product transfer station. The second guide section extends toward the bottom wall to guide the reagent kit 500 into the collection bucket 71. By including the first guide section and the second guide section in the guide groove 12, the first guide section can guide the reagent kit 500 to move horizontally within the extraction channel 11, while the second guide section, facing the bottom wall, can guide the reagent kit 500 to slide out of the second guide section and flip downwards, facilitating the disengagement of the reagent kit 500 from the hook portion 211 on the moving member 210, allowing the reagent kit 500 to fall into the collection bucket 71 after passing through the opening on the bottom wall under the action of gravity.

[0287] In some embodiments, the extraction channel 11 is provided with a leakage box 90 at the placement station for collecting leakage from the reagent kit 500. Since the reagent kit 500 is located at the placement station of the extraction channel 11, samples, reagents, or magnetic beads are added to the corresponding chambers of the reagent kit 500. Therefore, leakage may occur during the addition process, causing liquid to leak into the extraction channel 11. Therefore, by providing a leakage box 90 at the placement station, the leakage box 90 can collect the leakage from the reagent kit 500 during the liquid addition process, thereby improving the cleanliness of the gene extraction module 101.

[0288] When the reagent kit 500 in the extraction channel 11 moves to the product transfer station under the driving action of the moving part 210, and after the nucleic acid product in each batch of reagent kit 500 has been transferred, in order to avoid the waste liquid remaining in the reagent kit 500 after nucleic acid extraction from causing harm to personnel or the environment, a waste liquid treatment system 80 is also set up at the product transfer station before the reagent kit 500 enters the collection tank 71. The waste liquid treatment system 80 can extract and transfer the liquid remaining in the reagent kit 500, which can prevent the waste liquid from remaining in the reagent kit 500 and entering the collection mechanism 70 along with the reagent kit 500, thereby causing waste liquid to pollute the environment or affect human health.

[0289] In some embodiments, please refer to Figure 30 and Figure 31 The waste liquid treatment system 80 includes a cleaning head 81, a waste liquid reservoir 82, a cleaning agent reservoir 83, a first power unit, and a second power unit. The cleaning head 81 extends into the various cavities within the reagent kit 500. The waste liquid reservoir 82 is connected to the cleaning head 81 and is used to store waste liquid. The cleaning agent reservoir 83 is connected to the cleaning head 81 and is used to store cleaning agent. The first power unit provides power to enable the cleaning head 81 to extract waste liquid from the reagent kit 500 into the waste liquid reservoir 82. The second power unit provides power to enable the cleaning head 81 to inject cleaning agent into the container. The cleaning head 81 of this waste liquid treatment system 80 can both extract waste liquid and inject cleaning agent; one component performs multiple functions, simplifying the structure of the waste liquid treatment system 80 and reducing its cost. In other words, the waste liquid treatment system 80 has a simple structure and low cost.

[0290] The cleaning head 81 is a structure capable of drawing or injecting liquid under the action of a power unit. For example, the cleaning head 81 can be a cleaning needle. In this embodiment, the cleaning head 81 can draw waste liquid from the container to the waste liquid storage tank 82 under the action of the first power unit, and the cleaning head 81 can also inject cleaning agent from the cleaning agent storage tank into the container under the action of the second power unit.

[0291] It should be noted that the first power unit can be a pump body or a vacuum pumping device.

[0292] In some embodiments, the waste liquid treatment system 80 may include a drive mechanism connected to the cleaning head 81. The drive mechanism is used to adjust the position of the cleaning head 81 in space so that the cleaning head 81 can extend into or retract from the reagent kit 500. By providing a drive mechanism, it is easy to adjust the cleaning head 81 to the position of the reagent kit 500, so that the cleaning head 81 can extend into or retract from the reagent kit 500, increasing the flexibility of the waste liquid treatment system 80 in treating waste liquid in different locations of the reagent kit 500.

[0293] Optionally, please refer to Figure 30 The driving mechanism includes a fixed plate 84, a first horizontal driving mechanism 85, and a first vertical driving mechanism 86. The fixed plate 84 can be mounted on the outer wall of the extraction chamber 30, close to the product transfer station. The first horizontal driving mechanism 85 is connected to the fixed plate 84 and the first vertical driving mechanism 86, and can drive the first vertical driving mechanism 86 to move laterally relative to the frame. The first vertical driving mechanism 86 is connected to the cleaning head 81, and can drive the cleaning head 81 to move vertically relative to the fixed plate 84. Thus, through the cooperation of the first horizontal driving mechanism 85 and the first vertical driving mechanism 86, the cleaning head 81 can move both laterally and vertically.

[0294] It should be noted that "horizontal" here refers to the orientation in which the three gene extraction modules 101 are arranged. Therefore, the horizontal drive mechanism allows the washing head 81 to move between the three product transfer stations within the three gene extraction modules 101, enabling waste liquid to be aspirated from the reagent kit 500 at all three product transfer stations. "Vertical" here refers to the height of the reagent kit 500.

[0295] In some embodiments, the first lateral drive mechanism 85 may be a motor-driven pulley 231 mechanism, and the first vertical drive mechanism 86 may be connected to the transmission belt of the pulley 231 mechanism. The rotation of the driving pulley of the pulley 231 mechanism, through the cooperation of the driving and driven pulleys, enables the movement of the transmission belt, thereby driving the vertical moving mechanism to move laterally relative to the frame. To improve the stability of the vertical moving mechanism's movement, a laterally extending slide rail may be provided on the fixed plate 84, allowing the slide rail to slide against the vertical moving mechanism.

[0296] In some embodiments, the first vertical drive mechanism 86 is a lead screw and nut pair mechanism. The cleaning head 81 is connected to the nut and is driven vertically by the motor, so that the cleaning head 81 can approach and enter the corresponding hole in the reagent kit 500 to aspirate waste liquid.

[0297] It should be noted that the implementations of the first lateral drive mechanism 85 and the first vertical drive mechanism 86 are interchangeable. Furthermore, the first lateral drive mechanism 85 and the first vertical drive mechanism 86 can also include linear drive components such as linear electric cylinders, linear pneumatic cylinders, and linear hydraulic cylinders to directly drive lateral or vertical movement. Additionally, the drive mechanism can also include a longitudinal drive mechanism to achieve the longitudinal movement of the cleaning head 81. The specific structure of the longitudinal drive mechanism can be referred to the lateral and vertical drive mechanisms described above, and will not be repeated here. By setting the first longitudinal drive mechanism, where longitudinal refers to the length direction of the lysis well 5010 of the reagent kit 500, the cleaning head 81 can aspirate the waste liquid from the lysis well 5010, washing well 5012, and elution well 5013 within the reagent kit 500.

[0298] Thus, the washing head 81 can move along the horizontal, vertical and longitudinal directions, realizing three-axis movement of the washing head 81 in space. In this way, the washing head 81 can aspirate waste liquid from any well in the reagent kit 500 in the three gene extraction modules 101.

[0299] In some embodiments, the waste liquid treatment system 80 may further include a plurality of cleaning heads 81, which are mounted side by side on a nut frame and spaced apart laterally, and are all connected to the waste liquid reservoir 82 and the cleaning agent reservoir 83. By providing a plurality of cleaning heads 81, it is convenient to treat the waste liquid from multiple reagent chambers laterally on the reagent kit 500 simultaneously, thereby improving the treatment efficiency.

[0300] The distribution of the multiple washing heads 81 corresponds one-to-one with the arrangement of the multiple lysis wells 5010 in the width direction of the reagent kit 500. In this embodiment, when the reagent kit 500 is an eight-in-one consumable, i.e., the reagent kit 500 has eight lysis wells 5010, eight sets of washing wells 5012, and eight elution wells 5013, then the number of washing heads 81 on the movable seat can be set to eight. In this way, the eight washing heads 81 on the movable seat can simultaneously aspirate from the eight wells on the reagent kit 500. After the aspiration of the wells is completed, the washing heads 81 can move along the length direction of the lysis wells 5010, thereby aspirating the waste liquid in the lysis wells 5010, washing wells 5012, and elution wells 5013, reducing the waste liquid aspiration time, greatly improving the aspiration efficiency of the waste liquid treatment system 80, and correspondingly shortening the time required for nucleic acid extraction.

[0301] In some embodiments, please refer to Figure 31 The waste liquid treatment system 80 includes a first weighing sensor 87, a second weighing sensor 88, a controller, and an alarm. The first weighing sensor 87 detects the amount of waste liquid in the waste liquid storage tank 82 and can be placed at the bottom of the waste liquid storage tank 82. The second weighing sensor 88 detects the dosage of cleaning agent in the cleaning agent storage tank and can be placed at the bottom of the cleaning agent storage tank. The controller is electrically connected to the first weighing sensor 87 and the second weighing sensor 88, and is used to issue an alarm signal when the detected value of the first weighing sensor 87 is greater than a first threshold or the detected value of the second weighing sensor 88 is less than a second threshold. The alarm is electrically connected to the controller and is used to sound an alarm when it receives an alarm signal.

[0302] A load cell is a detection instrument capable of measuring the weight of a target component. Using a load cell to measure the amount of waste liquid in the waste liquid reservoir 82 and / or the amount of cleaning agent in the cleaning agent reservoir 83 offers high reliability at a low cost. Specifically, when the load cell detects that the weight in the waste liquid reservoir 82 is greater than a first threshold, it indicates that the amount of waste liquid in the waste liquid reservoir 82 is relatively large, approaching or having already reached its maximum storage capacity. At this time, the controller activates an alarm, prompting the user to drain the waste liquid from the waste liquid reservoir 82. When the load cell detects that the weight in the cleaning agent reservoir is less than a second threshold, it indicates that the amount of cleaning agent in the cleaning agent reservoir is low, approaching or having already been depleted. At this time, the controller activates an alarm, prompting the user to replenish the cleaning agent in the cleaning agent reservoir.

[0303] It should be noted that a controller is a master control device that controls the action of a target according to a predetermined program. Controllers can be CPUs (central processing units), ECUs (electronic control units), and PLCs (programmable logic controllers), etc.

[0304] A first weighing sensor 87 is used to detect the amount of waste liquid in the waste liquid storage tank 82, facilitating the monitoring of the waste liquid level. When the amount of waste liquid in the waste liquid storage tank 82 exceeds a first threshold, the controller activates an alarm to indicate that the waste liquid in the waste liquid storage tank 82 should be drained. A second weighing sensor 88 is used to detect the cleaning agent dosage in the cleaning agent storage tank, facilitating the monitoring of the cleaning agent dosage. When the cleaning agent dosage in the cleaning agent storage tank falls below a second threshold, the controller activates an alarm to indicate that the cleaning agent in the cleaning agent storage tank should be replenished.

[0305] In some embodiments, please refer to Figure 1 , Figure 18 and Figure 32 The PCR system construction area 900 and the sample loading area 200 are located on both sides of the nucleic acid extraction area 100. The PCR system construction area 900 includes a consumable area, a second reagent area 902, a second waste tip area 903, and a product placement area. The consumable area is used to place the consumables required for PCR system construction. The second reagent area 902 is used to store the reagents required for PCR system construction. The second waste tip area 903 is used to store the pipette tips 2011 after PCR system construction. The product placement area is used to store the products after PCR system construction. The PCR system construction area 900 is equipped with a second pipette 904, which is movably located in the PCR system construction area 900 and is used to transfer the products located in the product transfer area in the nucleic acid extraction area 100 to the PCR system construction area 900 for PCR system construction.

[0306] The nucleic acid extraction zone 100 spans the sample loading zone 200 and the PCR system construction zone 900. The reagent kit 500 enters the nucleic acid extraction zone 100 from the sample loading zone 200 and is passed all the way to the PCR system construction zone 900. The second pipette 904 in the PCR system construction zone 900 transfers the extracted product from the nucleic acid extraction zone 100 from the reagent kit 500 and places it on the system construction rack 906 for PCR system construction.

[0307] In some embodiments, please refer to Figure 18 A three-axis movement mechanism 905 is provided within the PCR system construction area 900. The three-axis movement mechanism 905 is connected to the second pipette 904 and is used to adjust the position of the second pipette 904 within the space on the worktable 1001. The three-axis movement mechanism 905 allows the second pipette 904 to move freely between the product transfer station and the PCR system construction area 900.

[0308] It should be noted that the structure of the three-axis moving mechanism 905 and the three-axis driving mechanism 702 located in the sample loading area 200 can be completely identical. The difference lies in their different locations: the three-axis moving mechanism 905 is located in the PCR system construction area 900, while the three-axis driving mechanism 702 is located in the sample loading area 200. The three-axis moving mechanism 905 also has a second lateral driving mechanism 7021, and the upright 7020 and the first slide rail in the second lateral driving mechanism of the three-axis moving mechanism 905 can extend into the PCR system construction area 900 for sharing with the second longitudinal driving mechanism 7022 of the three-axis moving mechanism 905. The second drive motor and the second pulley structure in the three-axis moving mechanism 905 are independently set as the second drive motor and the second pulley 231 mechanism in the three-axis drive mechanism 702. They only share the same first slide rail 70212, so that the longitudinal drive mechanism in the three-axis drive mechanism 702 and the second longitudinal drive mechanism in the three-axis moving mechanism 905 also operate independently.

[0309] Please see Figure 32 The system construction frame 906 can be a well plate structure with multiple construction wells. These wells are used for PCR system construction, and each well is used to hold the extract and reagents required for PCR system construction. The second pipette 904 can transfer the reagents in the second reagent area 902 and the extracted product at the product transfer station to the construction wells on the system construction frame 906, thereby enabling PCR system construction on the system construction frame 906.

[0310] In some embodiments, the second pipette tip area 901 has the same structure as the pipette tip 2011 holder in the first pipette tip area 201, both including a pipette tip 2011 holder for storing pipette tips 2011. The difference between the second pipette tip area 901 and the first pipette tip area 201 lies in their different positions on the workbench 1001. The first pipette tip area 201 is located in the sample loading area 200, while the second pipette tip area 901 is located in the PCR system construction area 900; therefore, the structure within the second pipette tip area 901 will not be described in detail here. Similarly, the second waste pipette tip area 903 has the same structure as the first waste pipette tip area 202, both including a waste pipette tip holder 2020 and a pipette tip collection container 2021. The difference between the second waste head area 903 and the first waste head area 202 lies in their positions on the workbench 1001. The first waste head area 202 is located in the sample loading area 200, while the second waste head area 903 is located in the PCR system construction area 900. Therefore, the structure of the second waste head area 903 will not be described in detail here. Furthermore, the reagent racks in the second reagent area 902 and the first reagent area 203 have the same structure.

[0311] PCR detection module: After the PCR system is constructed, PCR detection can be performed. The PCR detection module can be set up outside the workbench. Simply transfer the PCR system construction rack to the PCR detection module for PCR detection and finally output the results.

[0312] In summary, see the following: Figures 1-32 As shown, the working process of gene testing equipment is as follows:

[0313] First, arrange the consumables, reagents and samples on the workbench 1001. Add pipette tips 2011 to the first pipette tip area 201 of the sample area 200, place the pipette tips 2011 on the first pipette tip holder 2010, and add the reagents required for gene detection to the reagent rack in the first reagent area 203.

[0314] The sample loading module 300 loads the sample tube 3081. First, it pulls out the feed drawer 305, places the sample holder 308 containing the sample tube 3081 on the feed drawer 305, and then closes the feed drawer 305. Under the driving action of the hook in the first sample loading drive mechanism 302, the sample holder 308 on the feed drawer 305 is driven along the feed channel 3010 to the sampling station, so that the sample cup dispensing module 400 can take out the sample tube 3081 from the placement position and transfer it to the cap opening station for cap opening.

[0315] Then the sample cupping module 400 operates, and the capping drive mechanism in the sample cupping module 400 starts working, adjusting the position of the capping robot mechanism 406 and moving the capping robot mechanism 406 to the sample holder 308 at the sampling station. Then the bottle cap clamping component 4060 in the capping robot mechanism 406 operates, clamping the bottle cap of the sample tube 3081 located on the sample holder 308. Then, under the drive of the capping drive mechanism, the capping robot mechanism 406 moves from the sampling station to the capping station. During this process, the sample barcode scanner 407 scans and records the personnel information on the sample tube 3081 to achieve information-based detection. Then the capping robot mechanism 406 transfers the sample tube 3081 into the positioning hole 4050 of the cupping stage 401 at the capping station. Under the action of the clamping arm drive assembly 4052, the bottle clamping assembly 4051 at the positioning hole 4050 clamps the bottle body of the sample tube 3081 with two clamping arms. Then, with the bottle body clamped by the bottle clamping assembly 4051, the capping robot mechanism 406 drives the cap to rotate, thereby realizing the automatic opening of the sample tube 3081.

[0316] Simultaneously, the reagent loading module 600 loads reagent kits 500 onto the loading position of reagent kit 500 on the worktable 1001. During the addition of reagent kit 500, the reagent kit compartment 602 is pulled out from the bottom of the worktable 1001, facilitating the operator to add the reagent kit 500 into the compartment. After the reagent kit 500 is added, the reagent kit compartment 602 is retracted, positioned directly below the loading port of the worktable 1001. The lifting section 6030 in the reagent loading module 600 lifts the reagent kit 500 upwards, placing it at the loading position on the worktable 1001. The limiting component provides support for the reagent kit 500, which awaits gripping by the robotic arm 700 at the loading position.

[0317] Driven by the three-axis drive mechanism 702, the robotic arm 700 moves to the position directly above the reagent kit 500 loading station. The two mechanical grippers 7001 of the robotic arm 700 open and then descend to the gripping position, using the grippers 7001 to grasp the reagent kit 500 from both sides. The reagent kit 500 is then picked up from the loading station and transferred to the placement station in the nucleic acid extraction area 100. The robotic arm 700 then lowers the reagent kit 500 and moves away, leaving the reagent kit 500 to await the addition of nucleic acid samples and reagents. Simultaneously, the reagent kit loading module 600 operates, and the lifting unit 6030 continues to lift the next reagent kit 500 to the loading station, awaiting the next gripping by the robotic arm 700.

[0318] Under the action of the three-axis drive mechanism 702, the first pipette 701 first moves to the first pipette tip area 201 to pick up the pipette tip 2011. Then, the first pipette 701 moves to the already opened sample tube 3081 at the opening station. The first pipette 701 uses the pipette tip 2011 to pick up the sample in the sample tube 3081. Then, under the drive of the three-axis drive mechanism 702, the nucleic acid sample is transferred to the lysis well 5010 of the reagent kit 500 located at the placement station in the nucleic acid extraction area 100. Then, the first pipette 701 throws the pipette tip 2011 into the first waste tip area 202. Then, the first pipette 701 continues to move to the first tip area 201 to pick up tip 2011, and then moves to the first reagent area 203 to add the required reagents and magnetic beads to the corresponding wells in the kit 500. Tips 2011 are disposable, and one tip 2011 can be used to add lysis buffer to multiple lysis wells 5010 in the kit 500 one by one. Similarly, elution buffer can also be added using one tip 2011. Then, the nano-magnetic bead solution in the first reagent area 203 is added to the lysis wells 5010. After the nano-magnetic bead solution, required reagents, and nucleic acid samples in the kit 500 have all been added, wait for nucleic acid extraction.

[0319] Nucleic acid extraction steps: The door 51 of the extraction chamber 30 opens, and the gripping unit 21 moves along the extraction channel 11 to the placement station under the traction of the first drive assembly 23. The hook part 211 of the gripping unit 21 squeezes the reagent kit 500 and engages with the locking part 508 on the reagent kit 500. Then, under the action of the first drive assembly 23, the gripping unit 21 drags the reagent kit 500 along the extraction channel 11 in the first direction X to the extraction station. Before the gripping unit 21 moves to the extraction chamber 30, the front door 51 will automatically open under the action of the lifting assembly 52 under the action of the sensing sensor, so that the gripping unit 21 can drag the reagent kit 500 into the extraction chamber 30 together. Then the door 51 closes, making the extraction chamber 30 a sealed environment.

[0320] Then, driven by the third driving component 432, the magnetic rod sleeve mounting bracket 430 in the nucleic acid extraction mechanism 40 moves closer to the reagent kit 500 and comes into contact with the magnetic rod sleeve 431 on the reagent kit 500. The magnetic rod sleeve hole 5011 on the magnetic rod sleeve mounting bracket 430 and the magnetic rod sleeve 431 on the reagent kit 500 are connected and installed. Then, the magnetic rod sleeve 431 enters the lysis well 5010 and agitates the liquid in the lysis well 5010. At the same time, driven by the cam 62, the heating element 60 in the heating element 60 moves closer to the bottom of the reagent kit 500 and heats the bottom of the lysis well 5010 in the reagent kit 500 to promote the lysis of the nucleic acid sample. After the nucleic acid sample is lysed, the cam 62 rotates, and the heating element 60 descends and detaches from the bottom of the reagent kit 500 to dissipate heat effectively. Simultaneously, under the action of the second drive, the magnetic rod mounting bracket 420 drives the magnetic rod 421 to extend out of the magnetic rod sleeve 431 and cooperate with the magnetic rod sleeve 431, allowing the magnetic rod sleeve 431 to adsorb the magnetic beads. After a certain period of adsorption, the magnetic rod 421 and the magnetic rod sleeve 431 rise simultaneously and then descend into the washing hole 5012. Then, under the action of the second drive assembly 422, the magnetic rod 421 rises. Since the magnetic rod 421 inside the magnetic rod sleeve 431 is removed, the nano-magnetic beads attached to the surface of the magnetic rod sleeve 431 will redissolve in the washing liquid, washing the nucleic acid on the magnetic beads. This process will not be described in detail. Under the action of the nucleic acid extraction mechanism 40, the operations of lysis, multiple washing, and elution are completed in sequence. Among them, heating is required by the heating element 60 during lysis and elution. The transfer of magnetic beads requires the cooperation of both the magnetic rod 421 assembly and the magnetic rod sleeve 431 assembly.

[0321] After the nucleic acid product is extracted from the reagent kit 500, the rear door 51 is opened, and the reagent kit 500 is dragged to the product transfer station by the gripping unit 21. Once the reagent kit 500 reaches the product transfer station, the second pipette 904 in the PCR system construction area 900 picks up the pipette tip 2011 and transfers the extracted product from the reagent kit 500 to the system construction rack 906 for PCR system construction. Simultaneously, the waste liquid treatment system 80 activates, with the washing head 81 extending into each well of the reagent kit 500 to aspirate waste liquid from each well and pump it into the waste liquid storage tank 82. Then, the washing head 81 injects cleaning agent into the wells of the reagent kit 500 to clean it, and the cleaned waste liquid is pumped back into the waste liquid storage tank 82.

[0322] After product transfer and waste liquid aspiration, the reagent kit 500 continues to move backward under the drag of the gripping unit 21, moving from the product transfer station to the collection station. During this process, the pushing part 212 on the moving part 210 contacts and pushes the mating part 73 of the sliding door 72, thereby opening the sliding door 72 and exposing the opening. At the same time, the moving part 210 disengages the reagent kit 500 from the guide groove 12, and the other side of the reagent kit 500 also disengages from the hook part 211 of the moving part 210. Under the action of gravity, the reagent kit 500 automatically falls into the collection bucket 71 located below the opening. Then the moving part 210 moves back, and the sliding door 72 automatically closes under the action of the reset part 74, which can prevent the airflow below from entering the instrument and causing instrument contamination.

[0323] Using the second pipette 904, the extracted product is transferred from the kit 500 to the system construction frame 906. Then, the second pipette 904 uses tip 2011 to aspirate reagent from the second reagent area 902, introducing the reagent into the extracted product on the system construction frame 906 for PCR system construction. After PCR system construction is complete, PCR detection can be performed. The PCR detection module is used to detect nucleic acids, and the detection results are finally output.

[0324] It should be noted that, where there is no conflict, the features in the embodiments of this application can be combined with each other.

[0325] The above description is merely a preferred embodiment of this application and is not intended to limit this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the protection scope of this application.

Claims

1. A gene extraction module, characterized by, The nucleic acid extraction device comprises: a base comprising an extraction channel arranged to extend along a first direction, the extraction channel comprising, in sequence along the first direction, a placement station, an extraction station, a product transfer station, and a collection station, the placement station being configured to place a kit, the extraction station being configured to extract nucleic acid, the product transfer station being configured to transfer a product after extraction, and the collection station being configured to collect the kit after completion of the product transfer; a traction mechanism arranged on the base, the traction mechanism being configured to traction the kit to move along the first direction in the extraction channel, so that the kit sequentially passes through the extraction station and the product transfer station from the placement station to the collection station; a nucleic acid extraction mechanism arranged at the extraction station, the nucleic acid extraction mechanism being configured to extract nucleic acid from a sample in the kit; the traction mechanism comprises: a gripping unit movably arranged on the base along the first direction, the gripping unit being configured to grip and release the kit; a first driving assembly arranged on the base, the first driving assembly being configured to drive the gripping unit to reciprocally move along the first direction on the base; the gripping unit comprises: a moving member movably arranged on the base along the first direction; a hooking portion rotatably connected to the moving member, the hooking portion being provided with a guide slope and a locking groove; a resilient member arranged between the hooking portion and the moving member, the resilient member being configured to apply a resilient force to the hooking portion; when the moving member moves to the guide slope of the hooking portion and the locking portion of the kit is in contact with the guide slope, the locking portion can press the hooking portion and drive the hooking portion to rotate, so that the resilient member accumulates elastic potential energy; the elastic potential energy is used to drive the hooking portion to rotate back to reset the locking portion and the locking groove when the locking groove is engaged with the locking portion.

2. The gene extraction module of claim 1, wherein, The extraction channel comprises two side walls opposite along a second direction, the second direction being perpendicular to the first direction, the side walls being provided with guide grooves extending along the first direction, the guide grooves being configured to guide the kit to move in the extraction channel.

3. The gene extraction module of claim 1, wherein, The first driving assembly comprises a first driving member, a belt pulley, and a synchronous belt, the first driving member being mounted on the base, the first driving member being configured to drive the belt pulley to rotate, the synchronous belt being in transmission cooperation with the belt pulley and being arranged to extend along the first direction, the moving member being connected to the synchronous belt and moving along the first direction on the base under the action of the first driving member.

4. The gene extraction module of claim 2, wherein, The base is provided with an extraction cabin at the extraction station, the nucleic acid extraction mechanism being arranged in the extraction cabin, the extraction cabin being configured to separate the placement station from the product transfer station.

5. The gene extraction module of claim 4, wherein, The extraction cabin is formed with an avoiding opening between the bottom wall of the extraction channel and the two sides of the extraction cabin along the first direction, the avoiding opening being configured to allow the kit to enter or exit the extraction cabin.

6. The gene extraction module of claim 5, wherein, The extraction cabin is provided with a cabin door assembly at the avoiding opening, the cabin door assembly being configured to open and close the avoiding opening.

7. The gene extraction module of claim 6, wherein, The cabin door assembly comprises: a cabin door configured to close the avoiding opening; A lifting assembly is arranged in the extraction cabin and is configured to drive the cabin door to move along a third direction, which is perpendicular to the first direction and the second direction.

8. The gene extraction module of claim 7, wherein, The extraction cabin is provided with a second guide rail, and the cabin door is provided with a sliding block corresponding to one side of the extraction cabin. The sliding block is in sliding cooperation with the second guide rail.

9. The gene extraction module of claim 7, wherein, The extraction channel is provided with an induction sensor on one side close to the extraction cabin. The induction sensor is configured to sense the movement of the moving part into or out of the extraction station to control the operation of the cabin door assembly.

10. The gene extraction module of claim 4, wherein, The nucleic acid extraction mechanism comprises: A fixed frame is arranged in the base and located in the extraction cabin; A movable frame is movably arranged on the fixed frame along the first direction; A magnetic rod mounting frame is movably arranged on the movable frame along a third direction, and a magnetic rod is mounted on the magnetic rod mounting frame. The third direction is perpendicular to the first direction and the second direction; A magnetic rod sleeve mounting frame is movably arranged on the movable frame along the third direction. In the third direction, the magnetic rod sleeve mounting frame is closer to the extraction channel than the magnetic rod mounting frame; A second driving assembly is arranged on the movable frame, and the second driving assembly is configured to drive the magnetic rod mounting frame to move along the third direction; A third driving assembly is arranged on the movable frame, and the third driving assembly is configured to drive the magnetic rod sleeve mounting frame to move along the third direction; A fourth driving assembly is arranged on the fixed frame, and the fourth driving assembly is configured to drive the movable frame to move along the first direction on the fixed frame; Under the driving action of the second driving assembly and the third driving assembly, the magnetic rod can be inserted into the magnetic rod sleeve and into the reagent box located at the extraction station for magnetic attraction.

11. The gene extraction module of claim 10, wherein, The magnetic rod sleeve mounting frame is provided with a socket for inserting the magnetic rod sleeve. Under the action of the third driving assembly, the magnetic rod sleeve mounting frame can be inserted into the magnetic rod sleeve located in the reagent box, so that the magnetic rod sleeve located in the reagent box is mounted on the magnetic rod sleeve mounting frame.

12. The gene extraction module of claim 11, wherein, The socket is arranged along the third direction, and the inner wall of the socket is provided with a limiting groove. The outer wall of the magnetic rod sleeve is provided with a limiting protrusion matched with the limiting groove, so as to limit the axial movement of the magnetic rod sleeve on the magnetic rod sleeve mounting frame.

13. The gene extraction module of claim 12, wherein, The number of sockets on the magnetic rod sleeve mounting frame is multiple, and the multiple sockets are arranged in the second direction. The number of magnetic rods on the magnetic rod mounting frame is multiple, and the number and position of the multiple magnetic rods correspond to the multiple sockets one by one.

14. The gene extraction module of claim 11, wherein, The nucleic acid extraction mechanism further comprises a magnetic rod sleeve detection assembly arranged on the movable frame. The magnetic rod sleeve detection assembly is configured to detect the presence of the magnetic rod sleeve on the magnetic rod sleeve mounting frame.

15. The gene extraction module of claim 2, wherein, The gene extraction module further comprises: A heating element is arranged at the extraction station and is configured to heat at least part of the bottom of the reagent box.

16. The gene extraction module of claim 15, wherein, The heating element is movably arranged in the extraction channel along a third direction, which is perpendicular to the first direction and the second direction.

17. The gene extraction module of claim 16, wherein, The heating member has a first position in contact with the bottom of the kit and a second position separated from the bottom of the kit, and the gene extraction module further comprises: A fifth driving assembly is installed on the side wall, and the fifth driving assembly is used to drive the heating member to switch between the first position and the second position.

18. The gene extraction module of claim 17, wherein, The fifth driving assembly comprises: A cam in contact with the bottom of the heating member; A fifth driving member is installed on the side wall, and the fifth driving member is used to drive the cam to rotate so as to switch the heating member between the first position and the second position.

19. The gene extraction module of claim 2, wherein, The collection station is provided with a collection mechanism for collecting the kit, and the collection mechanism comprises: A collection barrel is arranged below the collection station, and the collection barrel is used to collect the kit after product transfer; One end of the guide groove extends between the placement station and the extraction station, and the other end of the guide groove extends between the product transfer station and the collection station; When the kit moves to the collection station, the kit is separated from the guide groove and falls into the collection barrel under the action of gravity.

20. The gene extraction module of claim 19, wherein, The bottom wall of the base has a collection cavity communicating with the outside, the collection barrel is located in the collection cavity, and the bottom wall is provided with an opening communicating with the extraction channel at the collection cavity; The collection mechanism further comprises: A sliding door assembly is movably arranged at the opening, and the sliding door assembly is used to close and open the opening.

21. The gene extraction module of claim 20, wherein, The sliding door assembly comprises: A sliding door is slidably arranged at the opening in the first direction; the side wall is provided with a sliding groove extending in the first direction near the bottom wall, the sliding groove is in sliding cooperation with the sliding door, and the sliding door has a closed state and an open state compared with the opening; A matching part is arranged on the sliding door, and a pushing part matched with the matching part is arranged on the moving part; Under the action of the moving part, the moving part pushes the matching part through the pushing part and drives the sliding door to slide in the first direction, so that the sliding door is switched from the closed state to the open state.

22. The gene extraction module of claim 21, wherein, A reset member is further arranged between the sliding door and the bottom wall, and the reset member is used to maintain the sliding door in the closed state.

23. The gene extraction module of claim 22, wherein, The number of reset members is two, and the two reset members are distributed in the second direction.

24. The gene extraction module of claim 23, wherein, The guide groove comprises a first guide section and a second guide section, the first guide section is arranged in the same direction as the first direction, and is used to guide the kit to move from the placement station to the product transfer station; and the second guide section extends towards the bottom wall, and is used to guide the kit into the collection barrel.

25. The gene extraction module of claim 1, wherein, The collection station is provided with a transfer mechanism, and the gene extraction module further comprises a collection unit, and the transfer mechanism is used to transfer the kit after completing product transfer to the collection unit.

26. The gene extraction module of claim 1, wherein, The extraction channel is provided with a liquid leakage box at the placement station for collecting liquid leakage on the kit.

27. A genetic testing device, comprising: It comprises: A frame; A workbench horizontally arranged on the frame, the workbench comprising a sample loading area and a nucleic acid extraction area, the sample loading area being used for placing sample tubes, reagent kits, pipette tips and reagents, and the nucleic acid extraction area being used for performing nucleic acid extraction; A mechanical arm installed on the workbench, the mechanical arm being used for transferring the reagent kit from the sample loading area to the nucleic acid extraction area; A first pipette, the first pipette being used for pipetting the sample in the sample loading area to the nucleic acid extraction area; and The nucleic acid extraction area is provided with at least one gene extraction module as claimed in any one of claims 1-26. In the nucleic acid extraction area, the number of gene extraction modules is multiple, and multiple gene extraction modules are arranged in a second direction perpendicular to the first direction.

28. The genetic testing device of claim 27, wherein, The sample loading area comprises a sample tube area, a reagent kit area, a first pipette tip area, a first reagent area and a first waste tip area, the sample tube area being used for storing and opening the sample tube, the reagent kit area being used for storing the reagent kit, the first pipette tip area being used for storing the pipette tip, the first reagent area being used for storing the reagent, and the first waste tip area being used for storing the used pipette tip.

29. The genetic testing device of claim 27, wherein the at least one genetic testing device is a microarray. The sample tube area comprises a sampling station and an opening station, the sampling station being used for providing a sample tube, and the opening station being provided with a sample cup dividing module, the sample cup dividing module being used for opening the cap of the sample tube.

30. The genetic testing device of claim 29, wherein, The sample cup dividing module comprises:

31. The genetic testing device of claim 30, wherein, A cup clamping mechanism arranged at the opening station, the cup clamping mechanism being used for clamping and fixing the bottle body of the sample tube; A cap rotating mechanical arm mechanism arranged at the sample tube area, the cap rotating mechanical arm mechanism being used for clamping the cap of the sample tube and being capable of rotating along the axis direction thereof to rotate and open the cap. The cup clamping mechanism comprises:

32. The genetic testing device of claim 31, wherein, A cup dividing table having a positioning hole for placing the sample tube; A bottle body clamping assembly arranged at the positioning hole, the bottle body clamping assembly comprising two clamping arms; A clamping arm driving assembly drivingly connected with at least one of the two clamping arms, the clamping arm driving assembly being used for driving the two clamping arms to approach each other to clamp the bottle body of the sample tube. The cap rotating mechanical arm mechanism comprises:

33. The genetic testing device of claim 31, wherein, A cap clamping assembly for clamping the cap of the sample tube; A cap rotating assembly drivingly connected with the cap clamping assembly, the cap rotating assembly being used for relatively rotating the cap of the sample tube along the axis direction thereof to separate the cap from the bottle body of the sample tube when the cup clamping mechanism clamps the bottle body of the sample tube. The sample cup dividing module further comprises an opening driving mechanism drivingly connected with the cap rotating mechanical arm mechanism, so that the cap rotating mechanical arm mechanism can move between the sampling station and the opening station to transfer the sample tube in the sampling station to the opening station for opening the cap.

34. The genetic testing device of claim 31, wherein, The sample cup dividing module further comprises a sample code scanner arranged on the side of the cap rotating mechanical arm mechanism, the sample code scanner being used for scanning and reading the identification code on the sample tube.

35. The genetic testing device of claim 31, wherein, ​ 36. The genetic testing device of claim 29, wherein, The kit area is provided with a kit feeding module, which is used to transport the kit to a kit loading position, and the mechanical hand is used to transfer the kit at the kit loading position to the placement station of the nucleic acid extraction area.

37. The genetic testing device of claim 36, wherein, The workbench is provided with a loading opening at the kit loading position, which is communicated with the outside, and the kit feeding module is arranged below the workbench, and is used to feed the kit from bottom to top through the loading opening to the kit loading position.

38. The genetic testing device of claim 37, wherein, The kit feeding module comprises: a base arranged below the workbench; a kit bin arranged on the base, the kit bin having a containing cavity for storing the kit, the containing cavity being arranged along a third direction, and the containing cavity being provided with a discharge opening on a side close to the loading opening for discharging the kit, the discharge opening being communicated with the loading opening; a jacking assembly arranged on the base, the jacking assembly being used to jack the kit in the containing cavity along the third direction to the kit loading position.

39. The genetic testing device of claim 38, wherein, The jacking assembly comprises: a lifting part for jacking the kit; a third screw rod distributed along the third direction, the lifting part being threadedly connected with the third screw rod; a third guide rail arranged on the base and extending along the third direction, the third guide rail being slidably connected with the lifting part to guide the movement of the lifting part; a third driving motor arranged on the base, the third driving motor being drivingly connected with the third screw rod, and the third driving motor being used to drive the third screw rod to rotate, so as to drive the lifting part to move in the containing cavity along the third direction, so that the kits in the kit bin are sequentially moved to the kit loading position.

40. The genetic testing device of claim 38, wherein, A limiting assembly is arranged at the loading opening, and the limiting assembly is used to support and limit the kit at the kit loading position, so as to prevent the kit at the kit loading position from falling into the kit bin.

41. The genetic testing device of claim 39, wherein, The kit feeding module further comprises: a sliding seat slidably connected with the base, the kit bin being mounted on the sliding seat, the sliding seat having a third position and a fourth position on the base; when the sliding seat is at the third position, the discharge opening of the kit bin is communicated with the loading opening; when the sliding seat is at the fourth position, the discharge opening of the kit bin is exposed outside the workbench, so as to facilitate adding kits into the kit bin; and the kit bin is provided with a gap hole on a side close to the lifting part, the gap hole being used to provide a gap when the kit bin is separated from the lifting part.

42. The genetic testing device of claim 39, wherein, The kit feeding module further comprises a limiting sensor arranged on the third guide rail, which is used to detect the running state of the lifting part.

43. The genetic testing device of claim 29, wherein, The first suction head area is provided with at least one set of first suction head racks, and a plurality of storage positions for storing the suction heads are arranged on the first suction head racks, and the suction heads are used for pipetting by the first pipette gun.

44. The genetic testing device of claim 27, wherein, The gene detection device further comprises: A PCR system construction area is arranged on the workbench, and the PCR system construction area and the sample loading area are respectively arranged on the two sides of the nucleic acid extraction area; the PCR system construction area comprises a consumable area, a second reagent area, a second waste head area and a product placement area, the consumable area is used for placing consumables required for PCR system construction, the second reagent area is used for storing reagents required for PCR system construction, the second waste head area is used for storing suction heads after being used for PCR system construction, and the product placement area is used for storing products after PCR system construction. The PCR system construction area is provided with a second pipette gun, and the second pipette gun is movably arranged in the PCR system construction area and used for transferring the products in the nucleic acid extraction area to the PCR system construction area for PCR system construction.

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