A device and method for capturing micro-nano particles in solution

Abstract

The present application belongs to the field of biology and micro-electromechanical systems, and specifically provides a device and method for capturing micro-nano particles in a solution. The device includes: a cover, a hollow microbody array, a microfluidic channel, a substrate and an acoustic excitation source; a groove is provided on the upper surface of the substrate, and the hollow microbody array is provided in the groove; wherein the depth of the groove is the same as the height of the hollow microbody array; the cover is bonded to one end of the hollow microbody array away from the groove surface; the cover is also bonded to the non-groove portion of the upper surface of the substrate to form the microfluidic channel; the microfluidic channel includes an inlet and an outlet; the acoustic excitation source is provided on the lower surface of the substrate, and the acoustic field range excited by the acoustic excitation source covers the hollow microbody array. Based on the technical solution provided in the present application, a lower frequency excitation source can be used to capture micro-nano particles.

Description

Technical Field

[0001] The present application relates to the fields of biology and micro-electromechanical systems, and in particular to a device and method for capturing micro-nano particles in a solution, and a method for replacing micro-nano particles in a solution. Background Art

[0002] Precise control of micro- and nano-sized particles (such as single cells and bacteria) can achieve the extraction of particles in solution and the replacement of particles in solution (i.e., replacing a particle in solution A with solution B), which is a basic operation for related applications in the fields of biomedical engineering, pharmaceutical technology, etc.

[0003] At present, the methods for manipulating micro-nano particles can be mainly divided into contact and non-contact methods. Contact manipulation has strong controllability and can provide a large manipulation force, but direct contact with micro-nano particles will cause contamination or even damage to the particles. Non-contact manipulation is mainly based on physical principles, including light (optical tweezers), magnetic field (magnetic tweezers), sound field (acoustic tweezers), etc. In non-contact manipulation technology, manipulation technology based on acoustic field does not require the manipulation target to have special properties such as light transmittance and ferromagnetism, and has good biocompatibility, which is highly praised by many scholars. The acoustic method is that the micro-nano particles in the sound field will exchange momentum and energy with the sound waves, thereby generating a manipulation force on the manipulated object. At this stage, the acoustic field involved in the acoustic manipulation method mainly uses high-frequency or ultra-high-frequency (30MHz-3000 MHz) sound fields, and uses its powerful acoustic radiation force or acoustic streaming effect to manipulate micro-nano particles. However, high-frequency devices have the disadvantages of complex processes and high costs, which seriously limits the popularization and industrialization of micro-nano manipulation chip technology. Summary of the invention

[0004] In view of the above problems of the prior art, the present application provides a device and method for capturing micro-nanoparticles in a solution, and a method for replacing micro-nanoparticles in a solution, so as to achieve the capture of micro-nano-level particles through a lower frequency acoustic excitation source, thereby simplifying the processing technology of the micro-nano manipulation chip, reducing costs, and improving the applicability of the chip.

[0005] To achieve the above-mentioned purpose, the first aspect of the present application provides a device for capturing micro-nanoparticles in a solution, comprising: a cover, a hollow microbody array, a microfluidic channel, a substrate and an acoustic excitation source; a groove is provided on the upper surface of the substrate, and the hollow microbody array is provided in the groove; wherein the depth of the groove is the same as the height of the hollow microbody array; the cover is bonded to one end of the hollow microbody array away from the groove surface; the cover is also bonded to the non-groove portion of the upper surface of the substrate to form the microfluidic channel; the microfluidic channel comprises an inlet and an outlet; the acoustic excitation source is provided on the lower surface of the substrate, and the acoustic field range excited by the acoustic excitation source covers the hollow microbody array.

[0006] Compared with solid structures of the same size, hollow structures have relatively low characteristic frequencies, making it easier to achieve relatively large vibration displacements under low-frequency conditions, thereby having stronger particle capture capabilities. In addition, hollow structures are more likely to excite a variety of vibration modes, thereby enriching the particle capture effect. Therefore, the device provided in this aspect can improve the particle capture capability and enrich the particle capture effect by setting up a hollow microbody array.

[0007] As a possible implementation manner of the first aspect, the hollow microbody array includes a plurality of hollow cylinders.

[0008] From the above, various micro-nanoparticle capture forms can be achieved by regulating the phase of the acoustic excitation source.

[0009] As a possible implementation manner of the first aspect, the hollow microbody array includes a plurality of hollow cuboids.

[0010] As described above, the micro-nanoparticles can be applied to positions where the capture efficiency can be improved.

[0011] As a possible implementation of the first aspect, the hollow microbody array includes a plurality of hollow rounded rectangular cylinders; wherein the rounded rectangular cylinders are three-dimensional structures with a rounded rectangle as a cross-section.

[0012] As a result, the micro-nanoparticles can be better concentrated in the central area of ​​the microstructure.

[0013] As a possible implementation of the first aspect, in the hollow microbody array: the height of the hollow microbody and the wall thickness of the hollow microbody are set according to the size of the target particle; wherein the outer diameter of the hollow microbody is smaller than the wavelength of the sound wave generated by the acoustic excitation source.

[0014] From the above, by adjusting the size of the hollow micro-body (such as height, wall thickness, etc.), the size of the particles that can be captured can be adjusted by geometric means, thereby improving the flexibility of the device.

[0015] As a possible implementation of the first aspect, the acoustic excitation source emits a first excitation wave to the hollow microbody array to cause the hollow microbody array to generate a first vibration; wherein the first vibration is a vibration phase of the hollow microbody in the y direction leading the vibration phase in the x direction by π / 2.

[0016] From the above, when the vibration phase of the hollow microbody in the y direction leads the vibration phase in the x direction by π / 2, the motion trajectory of the target particle presents a spiral motion trajectory toward the center of the circle, and after stabilization, it will become a circular motion around the hollow microbody, thereby achieving the capture of the target particle.

[0017] As a possible implementation of the first aspect, the acoustic excitation source emits a second excitation wave to the hollow microbody array to cause the hollow microbody array to generate a second vibration; wherein the second vibration is that the vibration phase of the hollow microbody in the y direction is the same as the vibration phase in the x direction.

[0018] From the above, when the vibration phase of the hollow microbody in the y direction is the same as the vibration phase in the x direction, the motion trajectory of the target particle presents a vortex motion trajectory, thereby achieving the capture of the target particle.

[0019] In addition, in this aspect, the acoustic flow pattern can be actively regulated by adjusting the vibration phase difference of the hollow microbody array, thereby enriching the particle capture effect.

[0020] As a possible implementation of the first aspect, the resonant frequency of the acoustic excitation source is 100 Hz-10 MHz.

[0021] From the above, based on the device provided in this application, the capture of micro-nanoparticles in the solution can be achieved at a frequency of 100 Hz-10 MHz, which greatly reduces the processing difficulty and cost of the acoustic tweezers device.

[0022] The second aspect of the present application provides a method for capturing micro-nanoparticles in a solution using the device provided in the first aspect, comprising: turning on an acoustic excitation source; injecting a sample solution containing target particles from an inlet of a microfluidic channel, wherein under the action of the acoustic excitation source, the hollow microbody array vibrates to cause the target particles to aggregate at the hollow microcolumns; and directing the remaining sample solution out of an outlet of the microfluidic channel.

[0023] As described above, first turn on the acoustic excitation source to generate acoustic excitation in the microfluidic channel, and then inject the sample solution into the microfluidic channel. Under the vibration of the hollow microbody array, the target particles in the sample solution will gradually gather around the hollow microbodies. When the aggregation concentration reaches the preset requirement, the remaining solution can be discharged from the sample outlet of the microfluidic channel, so that the target particles can be captured.

[0024] The third aspect of the present application provides a method for replacing micro-nanoparticles in a solution using the device provided in the first aspect, comprising: injecting the solution to be replaced from the inlet of the microfluidic channel; turning off the acoustic excitation source, and then releasing the target micro-nanoparticles captured by the hollow microbody array into the solution to be replaced, so that the solution to be replaced contains the target micro-nanoparticles, and obtaining a replacement solution; and exporting the replacement solution from the outlet of the microfluidic channel.

[0025] From the above, the premise of this method is that the first particles in the first sample solution have been captured and enriched around the hollow microbody. At this time, the second sample solution (i.e., the solution to be replaced) needs to be introduced into the microchannel, and the acoustic excitation source is turned off. At this time, the aggregated first particles will be released again into the second sample solution. When the degree of mixing reaches the preset requirements, the replaced solution can be discharged from the sample outlet of the microchannel, so that the replaced solution can be obtained.

[0026] These and other aspects of the present application will become more apparent from the following description of the embodiment(s). BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 A schematic diagram of the first overall structure of a device for capturing micro-nanoparticles in a solution provided in an embodiment of the present application;

[0028] Figure 2 A side view of a first overall structure of a device for capturing micro-nanoparticles in a solution provided in an embodiment of the present application;

[0029] Figure 3 A second overall structural schematic diagram of a device for capturing micro-nanoparticles in a solution provided in an embodiment of the present application;

[0030] Figure 4 A third overall structural schematic diagram of a device for capturing micro-nanoparticles in a solution provided in an embodiment of the present application;

[0031] Figure 5 A first schematic diagram of the acoustic flow and acoustic radiation force generated around a hollow cylinder provided in an embodiment of the present application;

[0032] Figure 6 A schematic diagram of capturing target particles by a first structure provided in an embodiment of the present application;

[0033] Figure 7 A second schematic diagram of the acoustic flow and acoustic radiation force generated around a hollow cylinder provided in an embodiment of the present application.

[0034] Figure 8 A schematic diagram of replacing target particles by the first structure provided in an embodiment of the present application;

[0035] Fig. 9 A working principle diagram of the second structure provided in the embodiment of the present application in the xz direction;

[0036] Fig.10 A working principle diagram of the second structure provided in the embodiment of the present application in the xy direction;

[0037] Fig.11 A schematic diagram of capturing target particles by a second structure provided in an embodiment of the present application;

[0038] Fig.12 A schematic diagram of replacing target particles by a second structure provided in an embodiment of the present application;

[0039] Fig.13 A diagram showing the working principle of the third structure in the xy direction provided in an embodiment of the present application. DETAILED DESCRIPTION

[0040] The words "first, second, third, etc." or module A, module B, module C and other similar terms in the specification and claims are only used to distinguish similar objects and do not represent a specific ordering of the objects. It is understandable that the specific order or sequence can be interchanged where permitted so that the embodiments of the present application described herein can be implemented in an order other than that illustrated or described herein.

[0041] In the following description, the numbers representing the steps, such as S110, S120, etc., do not necessarily mean that the steps must be executed in this manner. If permitted, the order of the previous and next steps can be interchanged, or they can be executed simultaneously.

[0042] The term "comprising" as used in the description and claims should not be interpreted as being limited to what is listed thereafter; it does not exclude other elements or steps. Therefore, it should be interpreted as specifying the presence of the features, integers, steps or components mentioned, but does not exclude the presence or addition of one or more other features, integers, steps or components and groups thereof. Therefore, the expression "a device comprising means A and B" should not be limited to a device consisting of components A and B only.

[0043] References to "one embodiment" or "an embodiment" in this specification mean that a particular feature, structure, or characteristic described in conjunction with the embodiment is included in at least one embodiment of the present application. Therefore, the phrases "in one embodiment" or "in an embodiment" appearing in various places in this specification do not necessarily refer to the same embodiment, but may refer to the same embodiment. In addition, in one or more embodiments, the particular features, structures, or characteristics can be combined in any appropriate manner, as would be apparent to one of ordinary skill in the art from this disclosure.

[0044] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present application. In case of any inconsistency, the meaning described in this specification or the meaning derived from the contents recorded in this specification shall prevail. In addition, the terms used herein are only for the purpose of describing the embodiments of the present application and are not intended to limit the present application.

[0045] Referring to the figures below, a device for capturing micro-nanoparticles in a solution provided in an embodiment of the present application is described in detail.

[0046] like Figure 1 , which is a schematic diagram of the overall structure of a device for capturing micro-nanoparticles in a solution provided in an embodiment of the present application, wherein a portion of the cover is cut open and planed open in order to display the hollow microbody array inside the groove. Figure 2 This is a side view of the device. Figure 1 and Figure 2 The structure of the device is described in detail. In this embodiment, the device for capturing micro-nano particles in a solution includes: a cover 110, a hollow microbody array 120, a microchannel 130, a substrate 140 and an acoustic excitation source 150. The cover 110 can be made of glass; the substrate 140 can be made of silicon.

[0047] The upper surface of the substrate 140 is provided with a groove. Figure 2 The side view of FIG. 1 more clearly shows the location of the groove. A hollow microbody array 120 is arranged inside the groove, and the top of the hollow microbody array 120 (i.e., the end of the hollow microbody array away from the upper surface of the groove) is bonded to the cover 110, and the cover 110 is also bonded to the non-groove area on the upper surface of the substrate 140, so that the cover 110, the substrate 140, and the groove on the upper surface of the substrate form a flow channel-like structure, i.e., the microchannel 130.

[0048] The microfluidic channel 130 includes an inlet 131 and an outlet 132. The inlet 131 is used to inject a sample solution containing target micro-nano particles, and the outlet 132 is used to output the remaining solution after the capture is completed.

[0049] The acoustic excitation source 150 is disposed on the lower surface of the substrate 140, and the acoustic field range excited by the acoustic excitation source 140 can cover the hollow microbody array 120. In this embodiment, the acoustic excitation source 150 can generally select an excitation source with a resonant frequency of 100 Hz-10 MHz, so as to achieve the use of low-frequency devices to drive the hollow microbody array 120 to vibrate, thereby forming an acoustic radiation effect and an acoustic streaming effect. For example, the acoustic excitation source can select a piezoelectric ceramic transducer (PZT) and the like.

[0050] The height of the hollow microbody array 120 is the same as the depth of the groove, that is, it can be understood that when the height of the hollow microcolumn is changed, the depth of the groove will also change accordingly. In addition, the height and wall thickness of the hollow microbody array 120 can be set according to the size of the target particle. Generally speaking, under the condition that other dimensions are the same, the higher the height of the hollow microbody array 120, the stronger the capture ability of the target particle and the better the capture effect. In addition, under the condition that other dimensions are the same, within a certain range, the acoustic flow velocity of the hollow microcolumn increases with the increase of the wall thickness, while the acoustic radiation force increases first and then decreases with the increase of the wall thickness. For the first vibration form, the capture ability of the target particle is measured by the combined effect of the acoustic radiation force and the acoustic flow, so there will be a certain optimal geometric size that makes the capture ability of the target particle the strongest. For the second vibration form, the capture ability of the target particle is measured by the acoustic radiation force, so the thicker the wall of the hollow microbody array 120, the weaker the capture ability of the target particle. The first vibration form is that the vibration phase of the hollow micro-body in the y direction leads the vibration phase in the x direction by π / 2, and the second vibration form is that the vibration phase of the hollow micro-body in the y direction is the same as the vibration phase in the x direction. The first vibration form and the second vibration form will be described in detail below.

[0051] As a possible implementation, the hollow microbody array 120 may include a plurality of hollow cylinders, namely Figure 1 The height of the hollow cylinder is the same as the depth of the groove on the upper surface of the substrate, and the inner diameter and outer diameter of the hollow cylinder can be set according to the size of the target micro-nano particles in the sample solution. It should be noted that the outer diameter of the hollow cylinder should be smaller than the wavelength of the sound wave generated by the acoustic excitation source 150.

[0052] As another possible implementation, the hollow microbody array 120 may also include a plurality of hollow cuboids, that is, Figure 3 When the hollow microbody array 120 is a plurality of hollow cuboids, the height, inner diameter and outer diameter of the hollow cuboids can be set with reference to the setting when the hollow microbody array 120 is a plurality of hollow cylinders, so it will not be described here. It should be noted that, since the hollow cuboid structure contains right-angle vertices, in this structure, a strong acoustic radiation force and a local acoustic flow field will be formed at the sharp wedge position (i.e., the right-angle position), causing some target micro-nanoparticles to gather near this position.

[0053] As another possible implementation, the hollow microbody array 120 may also include a plurality of hollow rounded rectangular cylinders; wherein the rounded rectangular cylinders are three-dimensional structures with a rounded rectangle as a cross section, and the rounded rectangle is formed by combining a rectangle and two semicircles. Figure 4The form shown. Among them, the settings of the height, inner diameter and outer diameter of the hollow rounded rectangular cylinder can all refer to the settings when the hollow microbody array 120 is a plurality of hollow cylinders, so it will not be repeated here. It should be noted that since the right-angle structure of the hollow cuboid is replaced by a rounded structure, the aggregation of target micro-nano particles at the right angle can be avoided. Based on the joints of the plurality of hollow rounded rectangular cylinders, more target micro-nano particles can be aggregated in the middle area of ​​the structure.

[0054] It should be understood that the hollow cylindrical structure, the hollow cuboid structure, or the hollow rounded rectangular cylindrical structure in the above examples are all examples of the hollow microbody array 120. In other embodiments, the hollow microbody array 120 may also be a structure of other shapes. It should be noted that the structure needs to be hollow, and the specific shape may be arbitrarily set, for example: a hollow triangular prism, that is, a hollow triangle in cross section, etc.

[0055] The following takes the hollow microbody array 120 including a plurality of hollow cylinders as an example to specifically explain the working principle of the device for capturing micro-nano particles in a solution.

[0056] First, the acoustic excitation source 150 is turned on. At this time, the substrate 140 forms a plate wave under the excitation of the acoustic excitation source 150. At the same time, the hollow microbody array 120 will also vibrate, thereby generating an acoustic field in the fluid, thereby forming an acoustic flow and an acoustic radiation force. Figure 5 FIG. 1 is a first schematic diagram of acoustic flow and acoustic radiation force generated around a hollow cylinder, wherein the acoustic excitation source 150 emits a first excitation wave to the hollow microbody array 120, causing the hollow microbody array 120 to generate a first vibration, and when the vibration phase of the hollow microbody in the y direction leads the vibration phase in the x direction by π / 2, a first acoustic flow mode and a first form of acoustic radiation force are formed. Figure 5 In the figure, 121 represents a hollow cylinder, 122 represents a first acoustic flow mode, i.e., a dotted circular track surrounding the hollow cylinder 121, and 123 represents a first form of acoustic radiation force, i.e., a circularly distributed radiation force pointing to the center of the circle (the center of the hollow cylinder 121).

[0057] like Figure 6As shown, a sample solution containing target particles 310 is then injected from the injection port 131 of the microfluidic channel. When passing through the hollow cylinder, under the action of the first form of acoustic radiation force 123, the target particles 310 move toward the center of the circle. At the same time, under the action of the first acoustic flow 122, the target particles 310 also move around the hollow cylinder 121. Therefore, the overall movement presents a spiral motion trajectory toward the center of the circle. After the target particles are enriched and stabilized, the target particles 310 will change to a circular motion around the hollow cylinder 121, so that the target particles 310 are gathered around the hollow cylinder.

[0058] When the aggregation degree of the target particles 310 meets the preset requirements, the remaining sample solution can be discharged from the sample outlet 132 of the microchannel, thereby achieving the capture of the target particles.

[0059] As another implementation method, a second excitation wave may be applied to the hollow microbody array 120 to cause the labeled particles to gather around the hollow cylinders. Figure 7 FIG. 1 is a second schematic diagram of acoustic flow and acoustic radiation force generated around a hollow cylinder, wherein the acoustic excitation source 150 emits a second excitation wave to the hollow microbody array 120, causing the hollow microbody array 120 to generate a second vibration, and when the vibration phase of the hollow microbody in the y direction is the same as the vibration phase in the x direction, a second acoustic flow mode and a second form of acoustic radiation force are formed. Figure 7 121 represents a hollow cylinder, 124 represents a second acoustic flow mode, i.e., four vortices are formed around the hollow cylinder 121, and 125 represents a second form of acoustic radiation force, i.e., a radiation force with a non-circular distribution toward the center of the circle. In the second acoustic flow mode, there is a critical radius δ related to the frequency of the acoustic excitation source 150. When the radius of the target particle is greater than the critical radius δ, the second form of acoustic radiation force 125 is dominant, and the target particle acts near the surface of the hollow cylinder; when the radius of the target particle is less than the critical radius δ, the Stokes drag induced by the second acoustic flow 124 is dominant, i.e., the motion trajectory of the target particle is to rotate around the hollow cylinder 121. Therefore, when the acoustic excitation source 150 sends a second excitation wave to the hollow microbody array 120, the acoustic flow mode can be actively controlled by adjusting the vibration phase of the hollow microbody array, so that different capture effects of different particles can be achieved.

[0060] It should be understood that the above two forms of excitation waves are only exemplary descriptions. In actual application, they can be arbitrarily selected according to the required capture effect. Other forms of excitation waves can also be selected to achieve other forms of capture effects.

[0061] The following takes the hollow microbody array 120 including a plurality of hollow cylinders as an example to specifically explain the working principle of the device for replacing micro-nano particles in a solution.

[0062] like Figure 8 As shown, first, the device is used to capture the target particle 310 in the solution, that is, the target particle 310 exists around the hollow cylinder. The capture process can be the same as the capture process of the target particle 310 described above, so it will not be described in detail.

[0063] Then, the solution to be replaced is injected from the injection port 131 of the microfluidic channel, and the acoustic excitation source 150 is turned off. At this time, since the acoustic excitation source is turned off, the vibration of the hollow cylinder will gradually disappear, so that the target particles 310 around the hollow cylinder are released again into the solution to be replaced, so that the solution to be replaced contains the target particles 310, and a replacement solution is obtained (the replacement solution contains the target particles 310).

[0064] The replacement solution is led out from the sample outlet 132 of the microfluidic channel, so that the replacement solution of the particles in the solution can be achieved.

[0065] like Fig. 9 FIG. 1 is a diagram showing the working principle of the device in the xz direction when the hollow microbody array 120 includes a plurality of hollow cuboids. Fig.10 The figure shows the working principle of the device in the xy direction when the hollow microbody array 120 includes a plurality of hollow cuboids. Under the excitation of the acoustic excitation source 150, an acoustic flow 412 and an acoustic radiation force 413 are generated around the hollow cuboid 411. It should be understood that the acoustic flow and acoustic radiation force in the figure are schematic drawings and only represent the nonlinear acoustic effect here. Fig.10 The focus is on demonstrating the acoustic flow 412 and acoustic radiation force 413 formed at the right-angle vertex.

[0066] Fig.11 Schematic diagram of the principle of capturing target particles when the hollow microbody array 120 includes a plurality of hollow cuboids. The same as the method of capturing target particles when the hollow microbody array 120 includes a plurality of hollow cylinders, firstly, the sample solution containing the target particles is injected from the injection port 131 of the microfluidic channel, the target particles 310 are positioned at a certain position of the hollow cuboids through the hollow cuboid array, and then the waste liquid is led out from the sample outlet of the microfluidic channel, thereby achieving the capture of the target particles 310.

[0067] Fig.12Schematic diagram of the principle of replacing target particles when the hollow microbody array 120 includes a plurality of hollow cuboids. The same method as the method of replacing target particles 310 when the hollow microbody array 120 includes a plurality of hollow cylinders is used, firstly, the target particles 310 are captured, and then the solution to be replaced is injected into the microchannel through the inlet 131 of the microchannel, and the acoustic excitation source 150 is turned off, and the target particles 319 around the hollow cuboids of the microchannel are released into the solution to be replaced again to obtain a replacement solution, and then the replacement solution is led out from the outlet 132 of the microchannel, thereby achieving the replacement of the target particles in the solution.

[0068] like Fig.13 The figure shows the working principle diagram of the device in the xy direction when the hollow microbody array 120 includes a plurality of hollow rounded rectangular cylinders 511. Under the excitation of the acoustic excitation source 150, an acoustic flow (not shown) and an acoustic radiation force 513 are generated around the rounded rectangular cylinder 511. It should be understood that the acoustic flow and acoustic radiation force in the figure are schematic drawings and only represent nonlinear acoustic effects. Under the action of the acoustic flow and acoustic radiation force 513, the target particles in the sample solution can be enriched in the central area of ​​the rounded rectangular cylinder 511.

[0069] It should be understood that when the hollow microbody array 120 includes a plurality of hollow rounded rectangular cylinders, the method and process of capturing and replacing target particles in the sample solution are the same as those of other shape structures, which can be referred to the above description and will not be repeated here.

[0070] Based on the device for capturing micro-nanoparticles in a solution, the method for capturing micro-nanoparticles, and the method for replacing micro-nanoparticles provided in the embodiments of the present application, it is possible to manipulate micro-nano-sized particles under a low-frequency acoustic excitation source of 100 Hz-10 MHz, thereby achieving the purpose of extracting and replacing micro-nanoparticles.

[0071] Note that the above are only preferred embodiments of the present application and the technical principles used. Those skilled in the art will understand that the present application is not limited to the specific embodiments described herein, and that various obvious changes, readjustments and substitutions can be made by those skilled in the art without departing from the protection scope of the present application. Therefore, although the present application is described in more detail through the above embodiments, the present application is not limited to the above embodiments, and may also include more other equivalent embodiments without departing from the concept of the present application, all of which belong to the protection scope of the present application.

Claims

1. A device for capturing micro-nanoparticles in a solution, characterized in that: include: A cover, a hollow microbody array, a microfluidic channel, a substrate and an acoustic excitation source; The upper surface of the substrate is provided with a groove, and the hollow microbody array is arranged in the groove; wherein the depth of the groove is the same as the height of the hollow microbody array; The cover is bonded to one end of the hollow microbody array away from the upper surface of the groove; the cover is also bonded to the non-groove portion of the upper surface of the substrate to form the microchannel; the microchannel includes an inlet and an outlet; The acoustic excitation source is disposed on the lower surface of the substrate, and the acoustic field range excited by the acoustic excitation source covers the hollow microbody array; In the hollow microbody array: the height of the hollow microbody and the wall thickness of the hollow microbody are both set according to the size of the target particle; wherein the outer diameter of the hollow microbody is smaller than the wavelength of the sound wave generated by the acoustic excitation source; The acoustic excitation source emits a first excitation wave to the hollow microbody array to cause the hollow microbody array to generate a first vibration; Among them, the first vibration is that the vibration phase of the hollow micro-body in the y direction leads the vibration phase in the x direction by π / 2.

2. The device according to claim 1, characterized in that The hollow microbody array includes a plurality of hollow cylinders.

3. The device according to claim 1, characterized in that The hollow microbody array includes a plurality of hollow cuboids.

4. The device according to claim 1, characterized in that The hollow microbody array comprises a plurality of hollow rounded rectangular cylinders; The rounded rectangular column is a three-dimensional structure with a rounded rectangle as its cross section.

5. The device according to claim 1, characterized in that The acoustic excitation source emits a second excitation wave to the hollow microbody array to cause the hollow microbody array to generate a second vibration; Wherein, the second vibration is that the vibration phase of the hollow micro-body in the y direction is the same as the vibration phase in the x direction.

6. The device according to claim 1, characterized in that The resonance frequency of the acoustic excitation source is 100 Hz-10 MHz.

7. A method for capturing micro-nanoparticles in a solution using the device according to any one of claims 1 to 6, characterized in that: include: Turn on the acoustic excitation source; A sample solution containing target particles is injected from the injection port of the microfluidic channel, and under the action of the acoustic excitation source, the hollow microbody array vibrates to cause the target particles to gather at the hollow microcolumns; The remaining sample solution is discharged from the sample outlet of the microfluidic channel.

8. A method for replacing micro-nanoparticles in a solution using the device according to any one of claims 1 to 6, characterized in that: include: Inject the solution to be replaced from the injection port of the microfluidic channel; When the acoustic excitation source is turned off, the target micro-nano particles captured by the hollow microbody array are released into the solution to be replaced, so that the solution to be replaced contains the target micro-nano particles, thereby obtaining a replacement solution; The replacement solution is discharged from the sample outlet of the microfluidic channel.

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