A method for scale conversion of phosphorus content in Yunnan pine seedlings

Abstract

The invention discloses a method for scale conversion of phosphorus content of Yunnan pine seedlings, comprising the following steps: obtaining seedling biomass, obtaining seedling phosphorus concentration, model independent variable testing, and biomass estimation model construction. The invention has the beneficial effects of: constructing a single-plant phosphorus content estimation model through seedling leaf biomass and leaf phosphorus concentration, and the phosphorus content of Yunnan pine seedlings can be quickly obtained, and the measurement process is convenient and accurate.

Description

Technical Field

[0001] The invention belongs to the technical field of nutrient element phosphorus content estimation research, and specifically relates to a method for scale conversion of phosphorus content in Yunnan pine seedlings. Background Art

[0002] Nitrogen and phosphorus are important nutrients for plants. There is a coupling relationship between the cycles of phosphorus and nitrogen, and phosphorus plays an important regulatory role in the nitrogen cycle. Therefore, the determination of phosphorus during plant growth and development is critical. The phosphorus content of each organ of the plant may be different, but there is a certain correlation. The phosphorus content of each organ constitutes the phosphorus content of the plant. This correlation can be used to determine the phosphorus content of organs that are easy to sample to estimate the phosphorus content of a single plant, thereby reducing the workload and reducing destructive sampling. Summary of the invention

[0003] The purpose of the present invention is to provide an intuitive and accurate method for converting the phosphorus content scale of Yunnan pine seedlings.

[0004] The technical solution adopted by the present invention is:

[0005] A method for converting the phosphorus content scale of Yunnan pine seedlings comprises the following steps:

[0006] (1) Obtaining seedling biomass: Leaves of Yunnan pine seedlings were collected, withered, and dried until the mass was constant. The leaf dry mass was measured to obtain the leaf biomass M. L Take the above leaves that have been dried to a constant mass and measure the phosphorus concentration to obtain the leaf phosphorus concentration C L ;

[0007] (2) Model independent variable test: Select the leaf biomass M L , leaf phosphorus concentration C L As an independent variable, leaf biomass M L , leaf phosphorus concentration C L Pearson correlation analysis was performed on the biomass and phosphorus content of various organs of Yunnan pine seedlings, and the correlations reached an extremely significant level. The results are shown in Table 1.

[0008] Table 1 Correlation between leaf biomass, leaf phosphorus content and other indicators of Yunnan pine seedlings

[0009]

[0010]

[0011] Note: ** indicates extremely significant correlation at the 0.01 level.

[0012] (3) Construction of biomass estimation model; leaf biomass M L is the independent variable, stem biomass M S, root biomass M R As the dependent variable, the power function is used to linearize the logarithm to build the model: log 10 Y=log 10 a+b log 10 X; leaf phosphorus concentration C L is the independent variable, stem phosphorus concentration C S , root phosphorus concentration C R As the dependent variable, a linear equation was selected to construct the model: Y = a + bX; according to the biomass of a single plant = leaf biomass + stem biomass + root biomass, M P =M L +M R +M S =M L +10 -0.301 M L 0.766 +10 -0.388 M L 0.907 The phosphorus content of a single plant was obtained as follows: P =M L C L +10 -0.301 M L 0.766 (0.1445C L +1.2897)+10 -0.388 M L 0.907 (0.4121C L +1.1353).

[0013] As a preferred method, the method also includes model accuracy verification; comparing the simulated values ​​with the measured values, drawing regression scatter plots between the measured values ​​of single plant biomass and the measured values ​​of single plant phosphorus content, and between the simulated values ​​of single plant biomass and the simulated values ​​of single plant phosphorus content, wherein the X-axis represents biomass, the Y-axis represents phosphorus content, and the middle oblique line (dashed line) represents the linear scatter regression relationship, and comparing the difference between the regression equation between the simulated values ​​and the measured values ​​and Y=a+bX; performing a significance comparison on the slope and intercept between the simulation curve and the verification curve, respectively, and finding that there is no significant difference between the slopes of the two curves (p=0.637>0.05), indicating that the prediction model does not change the scaling relationship between single plant biomass and single plant phosphorus content, and can better predict the single plant phosphorus content of Yunnan pine.

[0014] The beneficial effects of the present invention are:

[0015] By constructing a single-plant phosphorus content estimation model based on seedling leaf biomass and leaf phosphorus concentration, the phosphorus content of Yunnan pine seedlings can be quickly obtained. The measurement process is convenient and accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a linear relationship diagram between the measured value and the simulated value of the leaf biomass and the single plant biomass of the present invention;

[0017] Figure 2 It is a linear relationship diagram between the actual measured value and the simulated value of the single plant biomass and the single plant phosphorus content of the present invention. DETAILED DESCRIPTION

[0018] In order to enable those skilled in the art to understand the production process and technical effects of the present invention in detail, the application and technical effects of the present invention are further introduced with specific production examples below.

[0019] Example 1

[0020] The seedlings were dug up by the whole plant harvesting method. When digging, the root system was kept intact as much as possible. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of leaves, stems and roots of each sample was weighed with an electronic balance: 30.596g, 8.887g and 7.792g respectively. They were put into marked paper bags respectively, and after being fixed in an oven at 105℃ for 30min, the temperature was adjusted to 80℃ for drying until the weight was constant. The dry weight of each organ of leaves, stems and roots was measured to be 8.798g, 2.788g and 2.509g respectively. The biomass of a single plant (leaf biomass + stem biomass + root biomass) is MP = ML + MS + MR = 14.095g.

[0021] The dried leaf, stem and root samples were digested by H2SO4-H2O2 method, and the phosphorus concentration was determined by Nessler colorimetry to obtain the leaf phosphorus concentration C L , stem phosphorus concentration C S and root phosphorus concentration C R They are 2.012 mg / g, 0.543 mg / g and 3.180 mg / g respectively.

[0022] Phosphorus content of single Yunnan pine seedlings P = Leaf phosphorus content P L + Stem phosphorus content P S + Root phosphorus content P R ; Leaf phosphorus content P L = Leaf biomass M L × Leaf phosphorus concentration C L , stem phosphorus content P S = stem biomass M S × Stem phosphorus concentration C S , root phosphorus content P R = root biomass M R × Root phosphorus concentration C R ;

[0023] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =ML ×C L +M S ×C S +M R ×C R =8.798×2.012+2.788×0.543+2.509×3.180=27.192 mg.

[0024] Model independent variable test, leaf biomass M L , leaf phosphorus concentration C L As an independent variable, leaf biomass M L , leaf phosphorus content P L Pearson correlation analysis was performed with the biomass and phosphorus content of various organs of Yunnan pine seedlings;

[0025] Construction of biomass estimation model; leaf biomass M L is the independent variable, stem biomass M S , root biomass M R As the dependent variable, the power function is used to linearize the logarithm to build the model: log 10 Y=log 10 a+b log 10 X; leaf phosphorus concentration C L is the independent variable, stem phosphorus concentration C S , root phosphorus concentration C R As the dependent variable, a linear equation was selected to construct the model: Y = a + bX; according to the biomass of a single plant = leaf biomass + stem biomass + root biomass, M P =M L +M S +M R =M L +10 -0.388 M L 0.907 +10 -0.301 M L 0.766 The phosphorus content of a single plant was obtained as follows: P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897).

[0026] The leaf biomass obtained by applying the above method is (M L )8.798g, leaf phosphorus concentration is 2.012(CL )mg / g.

[0027] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =14.384g.

[0028] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897)=27.657mg.

[0029] Example 2

[0030] The whole plant harvesting method was used to dig out the seedlings, and the root system was kept intact as much as possible during digging. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of leaves, stems and roots of each sample was weighed with an electronic balance, and the weights were 9.702g, 3.536g and 4.257g respectively. They were placed in marked paper bags, dried in an oven at 105℃ for 30min, and then adjusted to 80℃ for drying until the weight was constant. The dry weights of leaves, stems and roots were measured to be 2.633g, 0.981g and 1.472g respectively.

[0031] Individual plant biomass (leaf biomass + stem biomass + root biomass), i.e. M P =M L +M S +M R =5.086g.

[0032] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =M L ×C L +M S ×C S +M R ×C R=2.633×2.138+0.981×1.103+1.472×1.601=9.068 mg.

[0033] The leaf biomass (M L )2.633g, leaf phosphorus concentration (C L ) is 2.138 mg / g.

[0034] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =4.668g.

[0035] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897)=9.293mg.

[0036] Example 3

[0037] The whole plant harvesting method was used to dig up the seedlings, and the root system was kept intact as much as possible during digging. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of the leaves, stems and roots of each sample was weighed with an electronic balance, and the fresh weights were 10.286g, 5.613g and 2.567g respectively. They were placed in marked paper bags, and after being dried in an oven at 105℃ for 30min, the temperature was adjusted to 80℃ for drying until the weight was constant. The dry weights of the leaves, stems and roots were measured to be 2.722g, 1.443g and 0.690g respectively.

[0038] Individual plant biomass (leaf biomass + stem biomass + root biomass), i.e. M P =M L +M S +M R =4.855g.

[0039] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =ML ×C L +M S ×C S +M R ×C R =2.722×2.028+1.443×1.531+0.690×1.933=9.062mg.

[0040] The leaf biomass (M L )2.722g, leaf phosphorus concentration (C L ) is 2.028 mg / g.

[0041] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =4.814g.

[0042] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897)=9.224mg.

[0043] Example 4

[0044] The whole plant harvesting method was used to dig out the seedlings, and the root system was kept intact as much as possible during digging. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of the leaves, stems and roots of each sample was weighed with an electronic balance, and the fresh weights were 33.111g, 13.001g and 7.746g respectively. They were put into marked paper bags, and after being dried in an oven at 105℃ for 30min, the temperature was adjusted to 80℃ for drying until the weight was constant. The dry weights of the leaves, stems and roots were measured to be 10.149g, 3.651g and 2.714g respectively.

[0045] Individual plant biomass (leaf biomass + stem biomass + root biomass), i.e. M P =M L +M S +MR =16.514g.

[0046] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =M L ×C L +M S ×C S +M R ×C R =10.149×2.220+3.651×1.445+2.714×2.218=33.818mg.

[0047] The leaf biomass (M L )10.149g, leaf phosphorus concentration (C L ) is 2.220 mg / g.

[0048] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =16.448g.

[0049] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897)=34.141mg.

[0050] Example 5

[0051] The seedlings were dug out by the whole plant harvesting method, and the root system was kept intact as much as possible during digging. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of leaves, stems and roots of each sample was weighed with an electronic balance, and the fresh weights were 23.093g, 8.593g and 8.901g respectively. They were put into marked paper bags, and after being dried in an oven at 105℃ for 30min, the temperature was adjusted to 80℃ for drying until the weight was constant. The dry weights of leaves, stems and roots were measured to be 5.712g, 1.815g and 2.265g respectively.

[0052] Individual plant biomass (leaf biomass + stem biomass + root biomass), i.e. M P =M L +M S +M R =9.792g.

[0053] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =M L ×C L +M S ×C S +M R ×C R =5.712×1.737+1.815×1.117+2.265×0.780=13.714 mg.

[0054] The leaf biomass obtained by applying the above method is (M L )5.712g, leaf phosphorus concentration is 1.737(C L )mg / g.

[0055] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =9.600g.

[0056] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897)=16.527mg.

[0057] Example 6

[0058] The whole plant harvesting method was used to dig out the seedlings, and the root system was kept intact as much as possible during digging. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of the leaves, stems and roots of each sample was weighed with an electronic balance, and the fresh weights were 32.350g, 12.080g and 15.244g respectively. They were put into marked paper bags, and after being dried in an oven at 105℃ for 30min, the temperature was adjusted to 80℃ for drying until the weight was constant. The dry weights of the leaves, stems and roots were measured to be 9.369g, 3.111g and 3.641g respectively.

[0059] Individual plant biomass (leaf biomass + stem biomass + root biomass), i.e. M P =M L +M S +M R =16.121g.

[0060] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =M L ×C L +M S ×C S +M R ×C R =9.369×2.092+3.111×0.866+3.641×1.870=29.101 mg.

[0061] The leaf biomass (M L )9.369g, leaf phosphorus concentration (C L ) is 2.092 mg / g.

[0062] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =15.258g.

[0063] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L 0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766(0.1445C L +1.2897)=30.236mg.

[0064] Example 7

[0065] The whole plant harvesting method was used to dig up the seedlings, and the root system was kept intact as much as possible during digging. The soil of the root system was carefully washed with water and dried in the shade. Each seedling was divided into leaves, stems and roots. The fresh weight of each organ of leaves, stems and roots of each sample was weighed with an electronic balance, and the weights were 19.453g, 7.532g and 6.639g respectively. They were placed in marked paper bags, dried in an oven at 105℃ for 30min, and then adjusted to 80℃ for drying until the weight was constant. The dry weights of leaves, stems and roots were measured to be 5.666g, 2.151g and 1.916g respectively.

[0066] Individual plant biomass (leaf biomass + stem biomass + root biomass), i.e. M P =M L +M S +M R =9.733g.

[0067] Phosphorus content of a single plant = leaf phosphorus content + stem phosphorus content + root phosphorus content, that is, P P =M L C L +M S C S +M R C R =5.666×2.189+2.151×0.904+1.916×1.777=17.753 mg.

[0068] The leaf biomass (M L )5.666g, leaf phosphorus concentration (C L ) is 2.189 mg / g.

[0069] Biomass of a single plant = leaf biomass + stem biomass + root biomass, M L +M S +M R =M L +10 -0.388 M L 0.907 +10 - 0.301 M L 0.766 =9.527g.

[0070] The phosphorus content of a single plant is: P P =M L C L +10 -0.388 M L0.907 (0.4121C L +1.1353)+10 -0.301 M L 0.766 (0.1445C L +1.2897)=19.457mg.

[0071] In the above Examples 2-7, except for the differences in the fresh weight, dry weight, biomass, and phosphorus content per plant of the tested leaves, stems, and roots, the detection methods used are the same as the methods and steps used in Example 1.

[0072] Model accuracy verification. Compare the simulated values ​​with the measured values, and draw regression scatter plots between the measured values ​​of single plant biomass and the measured values ​​of single plant phosphorus content, and between the simulated values ​​of single plant biomass and the simulated values ​​of single plant phosphorus content ( Figure 1-2 ), the X-axis represents biomass, the Y-axis represents phosphorus content, the middle oblique line (dashed line) represents the linear scatter regression relationship, and the difference between the regression equation between the simulated value and the measured value and Y=a+bX is compared ( Figure 1-2 ). The slope and intercept between the simulation curve and the verification curve were compared for significance, and it was found that there was no significant difference between the slopes of the two curves (p = 0.637> 0.05), indicating that the prediction model did not change the scaling relationship between the biomass of a single plant and the phosphorus content of a single plant, and could better predict the phosphorus content of a single plant of Yunnan pine.

[0073] The present invention proposes a method for converting the phosphorus content scale of Yunnan pine seedlings, comprising the following steps: (1) estimating the independent variables of the model and determining the dependent variables; selecting the seedling leaf biomass (M L ), leaf phosphorus concentration (C L ) is the independent variable, stem biomass (M S ), root biomass (M R ), biomass per plant (M P ), root phosphorus content (P R ), stem phosphorus content (P S ), phosphorus content per plant (P P ) is the dependent variable.

[0074] (2) Model selection: Use commonly used functions to construct an estimation model and determine a model with better fitting effect. The power function equation y=ax is selected for biomass. b To satisfy the homogeneity of variance, the power function is linearized by taking the logarithm, i.e. log 10 Y=log 10 a+blog 10 X. The linear equation Y=a+bX is selected for phosphorus content.

[0075] (3) Establishment of estimation model: Use leaf biomass to construct the relationship model between stem biomass, leaf biomass and individual plant biomass. Use leaf biomass and leaf phosphorus concentration as independent variables to construct the relationship model between them and stem phosphorus content, root phosphorus content and individual plant phosphorus content.

[0076] (4) Model accuracy assessment: The simulated values ​​obtained from the fitted model are fitted with the independent variables respectively, and the obtained model curve and verification curve are evaluated for accuracy from the perspective of slope and intercept.

[0077] From the above, it can be seen that by constructing a single-plant phosphorus content estimation model through seedling leaf biomass and leaf phosphorus concentration, the phosphorus content of Yunnan pine seedlings can be quickly obtained, and the measurement process is convenient and accurate.

[0078] Finally, it should be noted that the above embodiments are only used to illustrate rather than limit the technical solutions of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the present invention can still be modified or replaced by equivalents. Any modification or partial replacement that does not depart from the spirit and scope of the present invention should be included in the scope of the claims of the present invention.

Claims

1. A method for converting the phosphorus content scale of Yunnan pine seedlings, characterized in that: The following steps are involved: (1) Obtaining seedling biomass: Leaves of Yunnan pine seedlings were collected, withered, and dried until the mass was constant. The leaf dry mass was measured to obtain the leaf biomass M. L Take the above leaves that have been dried to a constant mass and measure the phosphorus concentration to obtain the leaf phosphorus concentration C L ; (2) Model independent variable test: Select the leaf biomass M L , leaf phosphorus concentration C L As an independent variable, leaf biomass M L , leaf phosphorus concentration C L Pearson correlation analysis was performed with the biomass and phosphorus content of various organs of Yunnan pine seedlings; (3) Construction of biomass estimation model; leaf biomass M L is the independent variable, stem biomass M S , root biomass M R As the dependent variable, the power function is used to linearize the logarithm to build the model: log 10 Y=log 10 a+blog 10 X; leaf phosphorus concentration C L is the independent variable, stem phosphorus concentration C S , root phosphorus concentration C R As the dependent variable, a linear equation was selected to construct the model: Y = a + bX; according to the biomass of a single plant = leaf biomass + stem biomass + root biomass, M P =M L +M R +M S =M L +10 -0.301 M L 0.766 +10 -0.388 M L 0.907 The phosphorus content of a single plant was obtained as follows: P =M L C L +10 -0.301 M L 0.766 (0.1445C L +1.2897)+10 -0.388 M L 0.907 (0.4121C L +1.1353).

2. The method for converting the phosphorus content of Yunnan pine seedlings according to claim 1, characterized in that: It also includes model accuracy verification; comparing the simulated values ​​with the measured values, drawing regression scatter plots between the measured values ​​of single plant biomass and the measured values ​​of single plant phosphorus content, and between the simulated values ​​of single plant biomass and the simulated values ​​of single plant phosphorus content, with the X-axis representing biomass, the Y-axis representing phosphorus content, and the middle oblique line representing the linear scatter regression relationship, and comparing the differences between the regression equations between the simulated values ​​and the measured values ​​and Y=a+bX; and making significance comparisons on the slope and intercept between the simulation curve and the verification curve.

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