A convenient nucleic acid detection device

Abstract

The application provides a nucleic acid convenient detection device, which comprises a test tube and a heating module. The test tube comprises: a test tube body, the top and bottom of which are both provided with openings, and the bottom opening is provided with a sealing cover; a flexible sealing cover, which is covered on the top of the test tube body to seal the top opening; a cavity formed by the test tube body, the flexible sealing cover and the sealing cover, which is a sealed cavity; a top rod, which is slidingly arranged along the length direction of the sealed cavity, and a reagent paper is preloaded in the top rod, the top of the top rod extends into the flexible sealing cover, and the bottom of the top rod is above the sealing cover; a base, which is detachably connected to the bottom of the test tube body, and a reagent cavity for placing a preloaded detection reagent is arranged in the base; the heating module is arranged below the base, and the heating module is used for heating the reagent cavity; the flexible sealing cover is pressed to push the top rod downward to break the sealing cover, so that the reagent liquid in the reagent cavity permeates upward along the reagent paper to display the detection result on the reagent paper. The detection device is convenient to operate and has high safety in use.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of assay, in particular to a nucleic acid convenient detection device. BACKGROUND

[0002] The substance of nucleic acid detection is the nucleic acid of virus, and the nucleic acid detection is to find whether the nucleic acid of the virus invading from outside exists in the respiratory tract specimen, blood or feces of the patient, so as to determine whether the patient is infected with the new coronavirus. Therefore, once the nucleic acid detection is "positive", it can prove that the patient has virus in the body. After the new coronavirus infects the human body, it will first reproduce in the respiratory system. Therefore, whether the human body is infected with the virus can be judged by detecting the viral nucleic acid in the respiratory epithelial exfoliated cells. Therefore, the positive nucleic acid detection can be used as the gold standard for the diagnosis of new coronavirus infection.

[0003] With the increasing normalization of nucleic acid detection, many people queue up for nucleic acid detection, which has become part of daily life. However, since there are many people queuing up for nucleic acid detection, if there is a person carrying the new coronavirus in the crowd, there is a risk of infection during the nucleic acid detection process, which is very unsafe. Moreover, it is very inconvenient to take a young child or baby to queue up for nucleic acid detection, and there is also a risk of infection. SUMMARY

[0004] The purpose of the present application is to provide a nucleic acid convenient detection device which is easy to operate and has high safety.

[0005] The present application provides a nucleic acid convenient detection device, which comprises a test tube, wherein the test tube comprises:

[0006] A test tube body, the top and bottom of the test tube body are both provided with openings, and the bottom opening of the test tube body is provided with a sealing cap;

[0007] A flexible sealing cover is arranged on the top of the test tube body to seal the top opening of the test tube body. The cavity formed by the test tube body, the flexible sealing cover and the sealing cap is a sealed cavity;

[0008] A top rod is slidably arranged along the length direction of the sealed cavity, and a reagent paper is preloaded in the top rod. The top of the top rod extends into the flexible sealing cover, and the bottom of the top rod is located above the sealing cap;

[0009] A base is detachably connected to the bottom of the test tube body, and a sealing structure is arranged between the base and the bottom of the test tube body. A reagent cavity for placing a preloaded detection reagent is arranged in the base;

[0010] When the flexible sealing cover is pressed, the flexible sealing cover is used to push the ejector rod downward to break the seal, so that the reagent liquid in the reagent cavity penetrates along the reagent paper and displays the detection result on the reagent paper.

[0011] Compared with the prior art, the nucleic acid convenient detection device is designed, the test tube includes a test tube body, a flexible sealing cover arranged at the top of the test tube body, an ejector rod arranged in the internal cavity of the test tube body and a base arranged at the bottom of the test tube body, the test tube body, the flexible sealing cover and the seal at the bottom of the test tube body form a sealed cavity, the ejector rod and the preloaded reagent paper are arranged in the sealed cavity, so that the reagent paper is not contaminated before detection, thereby ensuring the accuracy of the detection result and avoiding false positive results; meanwhile, the base is detachably connected to the bottom of the test tube body, a sealing structure is arranged between the base and the bottom of the test tube body, a reagent cavity for placing the preloaded detection reagent is arranged in the base, so that the preloaded detection reagent in the reagent cavity is not contaminated before use, in use, the base is removed, the nucleic acid sample to be detected is added and the base is reinstalled, the reagent cavity is heated by a heating device, the flexible sealing cover is flexible and deformed when pressed, so that the flexible sealing cover can push the ejector rod downward to break the seal, the reagent liquid penetrates along the reagent paper and displays the detection result on the reagent paper, the detection device and the detection method can ensure that there is no interference in the detection process and improve the accuracy of the detection result, and the detection device is convenient and fast in use, avoids queuing for nucleic acid detection and improves the safety of nucleic acid detection, which is conducive to the normalization of nucleic acid detection.

[0012] As a preferred scheme of the present application, the ejector rod includes a cylindrical rod body and a conical top head, the bottom of the top head is designed with a round corner, the top head is integrally connected to the bottom of the rod body, a guide strip is symmetrically arranged on the outer wall of the rod body along the length direction of the rod body, and a guide groove is arranged on the inner wall of the test tube body for the guide strip to slide along the length direction of the guide groove. Through the design of the structure of the ejector rod, the ejector rod includes a cylindrical rod body and a conical top head, a guide strip is arranged on the rod body to ensure smooth and stable movement of the ejector rod in the sealed cavity, and a guide groove is arranged on the inner wall of the test tube body to cooperate with the guide strip, so that the guide strip can slide along the length direction of the guide groove; the bottom of the top head is designed with a round corner, if a sharp corner is designed, the top head is relatively sharp, and the top head is easy to pierce the seal during transportation, resulting in product scrap, and if a round corner is designed, the top head is relatively blunt, so that the top head does not scratch the seal even if it slightly touches the seal during transportation, and the top head needs to be pressed to pierce the seal, thereby ensuring the integrity of the product during transportation.

[0013] Further, the rod body is provided with a containing groove along the length direction of the rod body for containing reagent paper, the top of the containing groove penetrates the top of the rod body, the containing groove is open to one side of the outer wall of the rod body, the test tube body is provided with a visual window arranged opposite to the opening, the visual window is a planar structure and is embedded on the test tube body. By designing the containing groove in the rod body, and the top of the containing groove penetrating the top of the rod body, the preloaded reagent paper is conveniently installed into the rod body from the top of the containing groove; the containing groove is open to one side of the outer wall of the rod body, so as to display the detection result on the reagent paper, the test tube body is provided with a visual window, so as to facilitate the user to observe the detection result, and the visual window is a planar structure instead of a curved surface structure, so as to facilitate accurate observation of the detection result.

[0014] Further, the inner wall of the containing groove at the opening position is provided with a symmetrical step for preventing the reagent paper from falling off from the opening of the containing groove, and the radial cross section of the containing groove is T-shaped. By designing the shape of the containing groove, the radial cross section of the containing groove is T-shaped, so that the inner wall of the containing groove at the opening position is provided with a symmetrical step for preventing the reagent paper from falling off from the opening of the containing groove, and the symmetrical step is used for limiting the position of the reagent paper in the containing groove.

[0015] Further, the outer wall of the top head is provided with a reagent liquid buffer groove in communication with the bottom of the containing groove, and the reagent liquid buffer groove is used for guiding the reagent liquid in the reagent cavity into the containing groove. When the top rod is pressed to pierce the seal, the top head is inserted into the reagent cavity, and in order to enable the reagent liquid to stably penetrate upward, the reagent liquid buffer groove is designed on the top head, which is also conducive to accurate and stable display of the detection structure.

[0016] Further, the bottom of the containing groove is provided with a limiting step for preventing the reagent paper from sliding off from the bottom end of the containing groove. Since the reagent paper is inserted into the top rod from the top of the containing groove, the bottom of the containing groove is in communication with the reagent liquid buffer groove, and if the connection between the bottom of the containing groove and the reagent liquid buffer groove is smooth and continuous, the reagent paper may slide off from the bottom of the containing groove. Therefore, the limiting step is designed to prevent the reagent paper from sliding off from the bottom end of the containing groove.

[0017] As another preferred embodiment of the present application, the rod body is provided with a deformable limiting rod at the upper part of the rod body, the limiting rod is arranged on the end face of the top opening of the test tube body, and the limiting rod is used for preventing the top rod from moving downward. By designing the deformable limiting rod at the upper part of the rod body, the limiting rod is arranged on the upper end face of the top opening of the test tube body when the limiting rod is not pressed, so as to prevent the top rod from mistakenly piercing the seal during transportation, and when the top rod is pressed downward, the limiting rod is deformed or broken, and the top rod can pierce the seal.

[0018] As a further preferred scheme of the present application, the base is threadedly connected to the outer wall of the bottom of the test tube body, and a sealing ring is sleeved on the outer wall of the bottom opening of the test tube body; or the base is threadedly connected to the inner wall of the bottom of the test tube body, and a sealing ring is sleeved on the outer wall of the base. By threadedly connecting the base to the test tube body, detachable connection between the base and the test tube body is achieved, and installation and disassembly are convenient. Moreover, the sealing ring is sleeved on the outer wall of the bottom opening of the test tube body, so that sealed connection between the base and the test tube body is ensured. Alternatively, an external thread is arranged on the base, an internal thread is arranged on the test tube body, the base is threadedly connected to the inner wall of the bottom of the test tube body, and a sealing ring is sleeved on the outer wall of the base. This sealing connection mode is also within the protection scope of the present application.

[0019] As a further preferred scheme of the present application, a heating module is further included, which comprises a fixing seat, an insertion slot for inserting the base is arranged on the upper surface of the fixing seat, a heat-conducting block for heating the reagent cavity is arranged in the insertion slot, and an electric heating block is arranged at the bottom of the heat-conducting block. By designing the heating module, the heating module comprises the fixing seat, the insertion slot for inserting the base is arranged on the upper surface of the fixing seat, and in use, the base is inserted into the insertion slot. The heat-conducting block in the insertion slot is heated by the electric heating block. The structure of the heat-conducting block is designed to be matched with the outer wall of the reagent cavity, so that the heating effect of the reagent cavity can be improved, and heating is uniform. In the present application, the electric heating block can be powered by a dry battery, a lithium battery or a USB interface.

[0020] As a further preferred scheme of the present application, the flexible sealing cover comprises a hard screw cap and a flexible sleeve, the hard screw cap is threadedly connected to the outer wall of the top opening of the test tube body, a hole is arranged in the middle of the hard screw cap, and the lower end of the flexible sleeve extends into the hole and is fixed in a circumferential sealing manner. By designing the structure of the flexible sealing cover, the flexible sealing cover comprises the hard screw cap and the flexible sleeve. The hard screw cap can be conveniently screwed on the test tube body, and the top rod is inserted into the flexible sleeve. In this way, when the flexible sleeve is pressed, the top rod can be pressed downward to break the seal. Moreover, the lower end of the flexible sleeve extends into the hole of the hard screw cap. When the hard screw cap is screwed to the top of the test tube body, the flexible sleeve extending into the hard screw cap is pressed against the edge of the top opening of the test tube body, so that the hard screw cap and the top opening of the test tube body are in sealed connection.

[0021] Further, the seal is a sealing film made of aluminum foil, or the seal is a thin plastic piece integrally formed on the inner wall of the bottom opening of the test tube body. The seal made of aluminum foil or the thin plastic piece integrally formed on the inner wall of the bottom opening of the test tube body is beneficial to breaking the seal by pressing the top rod, so as to ensure smooth nucleic acid detection. BRIEF DESCRIPTION OF DRAWINGS

[0022] Figure 1 This is a schematic diagram of the overall structure of the test tube in this invention;

[0023] Figure 2 This is a schematic diagram of the overall structure of the push rod in this invention;

[0024] Figure 3 This is a rear view of the overall structure of the test tube in this invention;

[0025] Figure 4 For along Figure 3 A cross-sectional view along the AA direction (the sealing film is made of aluminum foil);

[0026] Figure 5 For along Figure 3 A cross-sectional view along the AA direction (the seal is made of thin plastic).

[0027] Figure 6 This is a side view of the overall structure of the test tube in this invention;

[0028] Figure 7 For along Figure 6 A cross-sectional view along the BB direction (the sealing film is made of aluminum foil);

[0029] Figure 8 For along Figure 6 A cross-sectional view along the BB direction (the closure is made of thin plastic).

[0030] Figure 9 This is a schematic diagram of the connection structure between the heating module and the base in this invention.

[0031] Explanation of reference numerals in the attached figures:

[0032] 1. Test tube; 2. Test tube body; 2.1. Sealing; 2.2. Guide groove; 2.3. Viewing window; 3. Flexible sealing cap; 3.1. Hard screw cap; 3.2. Flexible sleeve; 4. Top rod; 4.1. Rod body; 4.1.1. Receptacle; 4.1.2. Symmetrical step; 4.1.3. Limiting rod; 4.1.4. Guide strip; 4.2. Top head; 4.2.1. Reagent buffer tank; 4.2.2. Limiting step; 5. Reagent paper; 6. Base; 6.1. Reagent chamber; 6.2. Sealing ring; 7. Heating module; 7.1. Fixing base; 7.2. Slot; 7.3. Heat-conducting block; 7.4. Electric heating block. Detailed Implementation

[0033] With reference to the drawings of the embodiments of the present application, the technical solutions in the embodiments of the present application will be described clearly and completely. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments of the present application, all the other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0034] As shown in Figure 1 and Figure 2 , the present application shows a nucleic acid convenient detection device, comprising a test tube 1 and a heating module 7, the heating module 7 is used for heating the bottom of the test tube 1, to ensure the smooth progress of the detection.

[0035] Among them, the test tube 1 comprises: a test tube body 2, a flexible sealing cover 3, a top rod 4 and a base 6.

[0036] As shown in Figure 3 , Figure 4 , Figure 6 and Figure 7 , the top and bottom of the test tube body 2 are both provided with openings, and the bottom opening of the test tube body 2 is provided with a sealing cover 2.1; the flexible sealing cover 3 covers the top of the test tube body 2 to seal the top opening of the test tube body 2; the cavity formed by the test tube body 2, the flexible sealing cover 3 and the sealing cover 2.1 is a sealed cavity; the top rod 4 is slidingly arranged along the length direction of the sealed cavity, and the top rod 4 is preloaded with a reagent paper 5, the top of the top rod 4 extends into the flexible sealing cover 3, and the bottom of the top rod 4 is located above the sealing cover 2.1; the base 6 is detachably connected to the bottom of the test tube body 2, and a sealing structure is arranged between the base 6 and the bottom of the test tube body 2, and the base 6 is provided with a reagent cavity 6.1 for placing a preloaded detection reagent; the heating module 7 is arranged below the base 6, and the heating module 7 is used for heating the reagent cavity 6.1; the flexible sealing cover 3 is pressed to push the top rod 4 downward to break the sealing cover 2.1, so that the reagent liquid in the reagent cavity 6.1 permeates upward along the reagent paper 5 to display the detection result on the reagent paper 5.

[0037] Specifically, as shown in Figure 2The top rod 4 comprises a cylindrical rod body 4.1 and a conical top head 4.2, and the bottom of the top head 4.2 is designed with a round corner, the top head 4.2 is integrally connected to the bottom of the rod body 4.1, and the outer wall of the rod body 4.1 is symmetrically provided with a guide strip 4.1.4 along the length direction of the rod body 4.1, and the inner wall of the test tube body 2 is provided with a guide groove 2.2 for the guide strip 4.1.4 to slide along the length direction. Through the design of the structure of the top rod 4, the top rod 4 comprises a cylindrical rod body 4.1 and a conical top head 4.2, in order to make the top rod 4 move smoothly and stably in the sealed cavity, the guide strip 4.1.4 is arranged on the rod body 4.1, the inner wall of the test tube body 2 is provided with the guide groove 2.2 matched with the guide strip 4.1.4, and the guide strip 4.1.4 can slide in the guide groove 2.2 along the length direction; the bottom of the top head 4.2 is designed with a round corner, if a sharp corner is designed, the top head 4.2 is relatively sharp, and in the transportation process, the sealing cover 2.1 is easily pierced, resulting in product scrap, and if a round corner is designed, the top head 4.2 is relatively blunt, and even if the sealing cover 2.1 is slightly touched in the transportation process, it will not be scratched, and the top head 4.2 needs to be pressed to pierce the sealing cover 2.1, thereby ensuring the integrity of the product in the transportation process.

[0038] A containing groove 4.1.1 for containing the reagent paper 5 is formed in the rod body 4.1 along the length direction, the top of the containing groove 4.1.1 penetrates the top of the rod body 4.1, the containing groove 4.1.1 is open to one side of the outer wall of the rod body 4.1, and the test tube body 2 is provided with a visible window 2.3 arranged opposite to the opening, the visible window 2.3 is a planar structure and is embedded in the test tube body 2. Through the design of the containing groove 4.1.1 in the rod body 4.1, and the top of the containing groove 4.1.1 penetrating the top of the rod body 4.1, it is convenient to install the preloaded reagent paper 5 into the rod body 4.1 from the top of the containing groove 4.1.1; the containing groove 4.1.1 is open to one side of the outer wall of the rod body 4.1, in order to display the detection result on the reagent paper 5, and the test tube body 2 is designed with the visible window 2.3, which is convenient for users to observe the detection result, and the visible window 2.3 is a planar structure rather than a curved surface structure, which is convenient for accurately observing the detection result.

[0039] The containing groove 4.1.1 is T-shaped in cross section perpendicular to the axis direction of the rod body 4.1, and the inner wall of the containing groove 4.1.1 at the opening position has a symmetrical step 4.1.2 for preventing the reagent paper 5 from falling out of the opening of the containing groove 4.1.1. Through the design of the shape of the containing groove 4.1.1, the containing groove 4.1.1 is T-shaped in cross section perpendicular to the axis direction of the rod body 4.1, so that the inner wall of the containing groove 4.1.1 at the opening position has a symmetrical step 4.1.2 for preventing the reagent paper 5 from falling out of the opening of the containing groove 4.1.1, and the symmetrical step 4.1.2 is used to limit the position of the reagent paper 5 in the containing groove 4.1.1.

[0040] The top head 4.2 is provided with a reagent liquid buffer groove 4.2.1 which is communicated with the bottom of the accommodating groove 4.1.1, and the reagent liquid buffer groove 4.2.1 is used to guide the reagent liquid in the reagent cavity 6.1 to the accommodating groove 4.1.1. When the top rod 4 is pressed to pierce the sealing cover 2.1, the top head 4.2 is inserted into the reagent cavity 6.1, and in order to make the reagent liquid penetrate upward stably, the reagent liquid buffer groove 4.2.1 is designed on the top head 4.2, which is also beneficial to the accurate and stable display of the detection structure.

[0041] The reagent liquid buffer groove 4.2.1 and the accommodating groove 4.1.1 are provided with a limiting step 4.2.2 which is used to prevent the reagent paper 5 from sliding off the bottom end of the accommodating groove 4.1.1. Since the reagent paper 5 is inserted into the top rod 4 from the top of the accommodating groove 4.1.1, the bottom of the accommodating groove 4.1.1 is communicated with the reagent liquid buffer groove 4.2.1, and if the connection between the bottom of the accommodating groove 4.1.1 and the reagent liquid buffer groove 4.2.1 is smooth and continuous, the reagent paper 5 may slide off the bottom of the accommodating groove 4.1.1, so the limiting step 4.2.2 is designed to prevent the reagent paper 5 from sliding off the bottom end of the accommodating groove 4.1.1.

[0042] In the application, the outer wall of the rod body 4.1 at the upper part of the rod body 4.1 is provided with a limiting rod 4.1.3 which can be deformed after being pressed, the limiting rod 4.1.3 is arranged on the end face of the top opening of the test tube body 2 before being pressed, and the limiting rod 4.1.3 is used to prevent the top rod 4 from mistakenly piercing the sealing cover 2.1. By designing the deformed limiting rod 4.1.3 on the upper part of the rod body 4.1, the limiting rod 4.1.3 is arranged on the upper end face of the top opening of the test tube body 2 before being pressed, which ensures that the top rod 4 will not mistakenly pierce the sealing cover 2.1 during transportation, and when the top rod 4 is pressed downward, the limiting rod 4.1.3 is deformed or broken, and the top rod can pierce the sealing cover 2.1.

[0043] In the embodiment, the base 6 is screw-connected to the bottom outer wall of the test tube body 2, and the outer wall of the bottom opening of the test tube body 2 is sleeved with a sealing ring 6.2; or the base 6 is screw-connected to the bottom inner wall of the test tube body 2, and the outer wall of the base 6 is sleeved with a sealing ring. By screw-connecting the base 6 to the test tube body 2, the detachable connection between the base 6 and the test tube body 2 is realized, and the installation and dismounting are convenient, and the sealing ring 6.2 is sleeved on the outer wall of the bottom opening of the test tube body 2, so that the sealing connection between the base 6 and the test tube body 2 is ensured; or the base 6 is provided with external threads, the test tube body 2 is provided with internal threads, the base 6 is screw-connected to the bottom inner wall of the test tube body 2, and the outer wall of the base 6 is sleeved with a sealing ring 6.2, and this sealing connection mode is also within the protection scope of the application.

[0044] In order to facilitate the installation and dismounting of the base 6, anti-skid lines are arranged on the outer wall of the bottom of the test tube body 2 and on the outer wall of the base 6, so as to increase the friction force of the hands when the base 6 is screwed or removed, thereby facilitating the operation.

[0045] The cavity structure of the reagent cavity 6.1 is adapted to the shape of the top head 4.2. Since the amount of the preloaded reagent in the reagent cavity 6.1 is small, the cavity structure of the reagent cavity 6.1 is designed to be adapted to the shape of the top head 4.2, so that when the top head 4.2 pierces the sealing cover 2.1 and is inserted into the reagent cavity 6.1, the reagent liquid in the reagent cavity 6.1 can be squeezed up as much as possible, so as to ensure that the reagent liquid is stably permeated to the reagent paper 5 to display the detection result.

[0046] In addition, the flexible sealing cover 3 comprises a hard screw cover 3.1 and a flexible sleeve 3.2, the hard screw cover 3.1 is threadedly connected with the top opening outer wall of the test tube body 2, and the middle portion of the hard screw cover 3.1 is provided with an opening, and the lower end of the flexible sleeve 3.2 extends into the opening and is circumferentially sealed and fixed with the opening. Figure 9 The heating module 7 comprises a fixing seat 7.1, the upper surface of the fixing seat 7.1 is provided with a slot 7.2 for inserting the base 6, the slot 7.2 is provided with a heat-conducting block 7.3 adapted to the outer wall of the reagent cavity 6.1, and the bottom of the heat-conducting block 7.3 is provided with an electric heating block 7.4. Through the design of the heating module 7, the heating module 7 comprises a fixing seat 7.1, the upper surface of the fixing seat 7.1 is provided with a slot 7.2 for inserting the base 6, in use, the base 6 is inserted into the slot 7.2, the heat-conducting block 7.3 in the slot 7.2 is heated by the electric heating block 7.4, and the structure of the heat-conducting block 7.3 is designed to be adapted to the outer wall of the reagent cavity 6.1, which can improve the heating effect of the reagent cavity 6.1 and heat uniformly. In the present application, the electric heating block 7.4 can be powered by a dry battery, a lithium battery or a USB interface.

[0047] In addition, the flexible sealing cover 3 comprises a hard screw cover 3.1 and a flexible sleeve 3.2, the hard screw cover 3.1 is threadedly connected with the top opening outer wall of the test tube body 2, and the middle portion of the hard screw cover 3.1 is provided with an opening, and the lower end of the flexible sleeve 3.2 extends into the opening and is circumferentially sealed and fixed with the opening.

[0048] In the present embodiment, the sealing cover 2.1 is an aluminum foil sealing film, such as Figure 4 and Figure 7 , or the sealing cover 2.1 is a thin sheet integrally formed on the inner wall of the bottom opening of the test tube body 2, such asFigure 5 and Figure 8 The sealing cover 2.1 is made of an aluminum foil or a thin plastic piece integrally formed with the inner wall of the opening at the bottom of the test tube body 2, and both are beneficial to the breaking of the sealing cover 2.1 after the pressing of the rod 4, so as to ensure the smooth progress of the nucleic acid detection.

[0049] In use, the base 6 is first rotated off the test tube body 2, then the nucleic acid sample to be detected is added into the reagent cavity 6.1 of the base 6, and then the base 6 is reinstalled at the bottom of the test tube body 2, and the base 6 is inserted into the slot 7.2 of the fixing seat 7.1 of the heating module 7, and after heating by the electric heating block 7.4, the flexible cover 3.2 is pressed downward to push the rod 4 to break the sealing cover 2.1, and the top head 4.2 of the rod 4 is inserted into the reagent cavity 6.1, and the reagent liquid in the reagent cavity 6.1 permeates upward along the reagent paper 5 preloaded in the rod 4, and the detection result is displayed on the reagent paper 5, and the detection result can be checked by the detection person through the visual window on the side of the test tube body 2, which is very convenient.

[0050] In summary, the present application has the following advantages:

[0051] 1) The test tube comprises a test tube body 2, a flexible sealing cover 3 arranged at the top of the test tube body 2, a rod 4 arranged in the internal cavity of the test tube body 2, and a base 6 arranged at the bottom of the test tube body 2, the test tube body 2, the flexible sealing cover 3 and the sealing cover 2.1 at the bottom of the test tube body 2 form a sealed cavity, the rod 4 and the preloaded reagent paper 5 are arranged in the sealed cavity, so that the reagent paper 5 is not contaminated before detection, and the accuracy of the detection result can be ensured, and false positive results can be avoided;

[0052] 2) The base 6 is detachably and sealingly connected to the bottom of the test tube body 2, and the reagent cavity 6.1 for placing the preloaded detection reagent is arranged in the base 6, in use, the base 6 is first removed, the nucleic acid sample to be detected is added, and the base 6 is reinstalled, and the reagent cavity 6.1 is heated by the heating module 7, since the flexible sealing cover 3 is flexible and will be deformed when pressed, the pressing of the flexible sealing cover 3 can push the rod 4 downward to break the sealing cover 2.1, the reagent liquid will permeate upward along the reagent paper 5 and display the detection result on the reagent paper 5, the use of the detection device and the detection method can ensure that there is no interference in the detection process, improve the accuracy of the detection result, and the detection device is convenient and fast in use, and nucleic acid detection does not need to be queued, so that the risk of poor infection during queuing is avoided, the safety of nucleic acid detection is improved, and the normalization of nucleic acid detection is facilitated;

[0053] 3) The heating module 7 comprises a fixed seat 7.1, the upper surface of the fixed seat 7.1 is provided with a slot 7.2 for inserting the base 6, in use, the base 6 is inserted into the slot 7.2, the heat-conducting block 7.3 in the slot 7.2 is heated by the electric heating block 7.4, the heat-conducting block 7.3 is designed to be matched with the outer wall of the reagent cavity 6.1, which can improve the heating effect of the reagent cavity 6.1, and the heating is uniform, and the electric heating block 7.4 can be powered by a dry battery, a lithium battery or through a USB interface, which is also very convenient and low in cost.

[0054] It should be noted that all directional indications, such as upper, lower, left, right, front, back, inner, outer, etc., in the embodiments of the present application are only used to explain the relative positional relationship, movement condition, etc. between components in a certain specific posture (as shown in the drawings), and if the specific posture changes, the directional indications will also change accordingly.

[0055] In the present application, unless otherwise explicitly specified and limited, the terms "mounting", "connecting", "fixing" and the like should be understood in a broad sense, for example, can be fixed connection, can also be detachable connection, or integrated; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through an intermediate medium, can be the internal communication of two elements or the interaction relationship between two elements. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0056] The above is only the preferred embodiment of the present application, and does not limit the implementation and protection scope of the present application. Those skilled in the art should be able to realize that any equivalent replacement and obvious changes made according to the content of the present application should be included in the protection scope of the present application.

Claims

1. A nucleic acid convenient detection device, characterized by, The test tube (1) comprises: a test tube body (2), the top and bottom of which are both open, and the bottom of which is provided with a sealing cap (2.1); a flexible sealing cap (3) covering the top of the test tube body (2) to seal the top opening of the test tube body (2); the cavity enclosed by the test tube body (2), the flexible sealing cap (3) and the sealing cap (2.1) is a sealed cavity, the flexible sealing cap (3) comprises a hard screw cap (3.1) and a flexible sleeve (3.2), the hard screw cap (3.1) is threadedly connected with the outer wall of the top opening of the test tube body (2), the middle part of the hard screw cap (3.1) is provided with an opening, and the lower end of the flexible sleeve (3.2) extends into the opening and is fixedly connected with the opening in a circumferential sealing manner; a top rod (4) slidingly arranged along the length direction of the sealed cavity, and a reagent paper (5) being preloaded in the top rod (4), the top of the top rod (4) extending into the flexible sealing cap (3), and the bottom of the top rod (4) being located above the sealing cap (2.1), the top rod (4) comprising a rod body (4.1) and a top head (4.2), the top head (4.2) being arranged at the bottom of the rod body (4.1), and a containing groove (4.1.1) for containing the reagent paper (5) being formed in the rod body (4.1) along the length direction of the rod body (4.1), and a reagent liquid buffer groove (4.2.1) being formed in the outer wall of the top head (4.2) and being communicated with the bottom of the containing groove (4.1.1); a base (6) detachably connected with the bottom of the test tube body (2), and a sealing structure being arranged between the base (6) and the bottom of the test tube body (2), and a reagent cavity (6.1) for placing a preloaded detection reagent being arranged in the base (6); when the flexible sealing cap (3) is pressed, the flexible sealing cap (3) is used for pushing the top rod (4) downward to break the sealing cap (2.1), so that the reagent liquid in the reagent cavity (6.1) permeates along the reagent paper (5) upward and displays a detection result on the reagent paper (5), and the reagent liquid buffer groove (4.2.1) is used for guiding the reagent liquid in the reagent cavity (6.1) into the containing groove (4.1.1).

2. The nucleic acid convenient detection device according to claim 1, characterized in that: A guide strip (4.1.4) is arranged on the outer wall of the rod body (4.1) along the length direction of the rod body (4.1), and a guide groove (2.2) is arranged on the inner wall of the test tube body (2) and used for sliding the guide strip (4.1.4) along the length direction of the guide groove (2.2).

3. The nucleic acid convenient detection device according to claim 2, characterized in that: The top of the containing groove (4.1.1) penetrates the top of the rod body (4.1), the containing groove (4.1.1) is open to one side of the peripheral wall of the rod body (4.1), and a visible window (2.3) is arranged on the test tube body (2) and opposite to the opening.

4. The nucleic acid convenient detection device according to claim 3, characterized in that: The inner wall of the accommodation groove (4.1.1) in the open position is provided with symmetrical steps (4.1.2) for preventing the reagent paper (5) from falling from the opening of the accommodation groove (4.1.1), and the accommodation groove (4.1.1) is T-shaped along the radial cross-section of the rod body (4.1).

5. The nucleic acid convenient detection device according to claim 3, characterized in that: The bottom of the accommodation groove (4.1.1) is provided with a limiting step (4.2.2) for preventing the reagent paper (5) from sliding from the bottom end of the accommodation groove (4.1.1).

6. The nucleic acid convenient detection device according to any one of claims 2-5, characterized in that: The rod body (4.1) is provided with a limiting rod (4.1.3) at the upper part of the rod body (4.1), which can be deformed after pressing, and the limiting rod (4.1.3) is arranged on the end face of the top opening of the test tube body (2), and the limiting rod (4.1.3) is used to prevent the top rod (4) from moving downward.

7. The nucleic acid convenient detection device according to claim 1, characterized in that: The base (6) is threadedly connected to the bottom outer wall of the test tube body (2), and a sealing ring (6.2) is sleeved on the outer wall of the bottom opening of the test tube body (2); or the base (7) is threadedly connected to the bottom inner wall of the test tube body (2), and a sealing ring is sleeved on the outer wall of the base (7).

8. The nucleic acid convenient detection device according to claim 6, characterized in that: It also includes a heating module (7), which includes a fixing seat (7.1), and the upper surface of the fixing seat (7.1) is provided with a slot (7.2) for inserting the base (6), and the slot (7.2) is provided with a heat-conducting block (7.3) for heating the reagent cavity (6.1), and the bottom of the heat-conducting block (7.3) is provided with an electric heating block (7.4).

9. The nucleic acid convenient detection device according to claim 1, characterized in that: The sealing cover (2.1) is a sealing film made of aluminum foil, or the sealing cover (2.1) is a thin sheet integrally formed on the inner wall of the bottom opening of the test tube body (2).

Images