A method for preparing silk fibroin contact lenses based on natural silk
Preparing silk fibroin contact lenses through natural silk solves the problem of poor breathability of existing contact lenses, and achieves the preparation of high oxygen permeability lenses, protects the health of the eyeballs and has environmental advantages.
Patent Information
- Application Number
- CN202210948520.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2022-08-09
- Publication Date
- 2025-04-08
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing contact lenses have poor breathability. Long-term wearing will lead to hypoxia in the cornea, causing redness, pain, dryness and even reduced vision.
Silk fibroin contact lenses are prepared using natural silk. By removing the glue in the silk, mixing them with lithium bromide solution, the silk protein solution is obtained, and then mixed with acrylamide through dialysis and dripping into the contact lens mold, and air-drying to obtain the lens.
It provides contact lenses with high oxygen permeability to avoid hypoxia in the cornea, reduces redness, pain and dryness in the eyes, and protects the wearer's eye health. At the same time, due to the natural source and degradability of the material, it has environmental advantages.
Smart Images
Figure CN115128839B_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of contact lenses, and in particular relates to a method for preparing silk fibroin contact lenses based on natural silk. Background Art
[0002] Contact lenses, also known as contact lenses, are a tool worn on the cornea of the eye to correct and protect vision. Contact lenses provide a wide field of vision and realistic vision, which can help control the development of myopia and astigmatism in adolescents. Some of them also have the function of treating and preventing eye diseases. Contact lenses can help myopic patients get rid of the shackles of frame glasses and have the function of cosmetic decoration, so they are sought after by many young people. However, the basic materials of contact lenses are still mainly polymer hydrogels and silicone hydrogels. This causes the material of contact lenses to pose a threat to the environment. In addition, many contact lenses have poor air permeability, and long-term wear can cause corneal hypoxia, causing redness, swelling, pain, dryness and even decreased vision in the eyes. Summary of the invention
[0003] The technical problem to be solved by the present invention is to provide a method for preparing silk fibroin contact lenses based on natural silk, so as to solve the technical problems that the contact lenses in the prior art have poor air permeability, long-term wearing can cause corneal hypoxia, and cause redness, swelling, pain, dryness and even decreased vision in the eyes.
[0004] The technical solution of the present invention is:
[0005] A method for preparing silk fibroin contact lenses based on natural silk, the method comprising: degumming natural silk with a potassium hydroxide solution and then dissolving it with lithium bromide to obtain a silk fibroin solution; dialyzing the silk fibroin solution and mixing it with acrylamide, then dripping it into a contact lens mold, and air-drying to obtain a lens.
[0006] The lens is the reaction product of lithium bromide, acrylamide, and silk fibroin.
[0007] The silk protein solution was dialyzed in sterile ultrapure water for 4 to 6 days, and the sterile ultrapure water was replaced every 6 to 24 hours.
[0008] The contact lens mold is washed and dried with sterile water, and then a mixed solution of acrylamide and silk fibroin is added; the air-drying process temperature is 10-40 o C.
[0009] The potassium hydroxide solution is 0.3% to 3% (w / v), the concentration of the lithium bromide solution is 1% to 10% (g / mL), and the concentration of the acrylamide solution is 2.5% to 7.5% (g / mL); the acrylamide solution and the silk protein solution are mixed at a volume ratio of 1:10.
[0010] The silk protein solution is a solution obtained by mixing natural silk with a lithium bromide solution after degumming. The preparation method is as follows: after the impurities are picked out of the silk, the silk is placed in an alkaline solution for degumming, and then mixed with the lithium bromide solution and stirred to break the hydrogen bond network in the silk fiber, thereby dissolving the silk fibroin into a silk protein solution and reprocessing it, and then removing the lithium bromide and soluble impurities contained in the silk protein solution through a dialysis method.
[0011] The lens is a silk fibroin film induced by acrylamide molecules, and the preparation method is: mixing acrylamide molecules with silk fibroin solution to induce amorphous silk fibroin to form a crystalline and stable silk fibroin film, and then removing the acrylamide by repeated soaking and rinsing.
[0012] The concentration of silk protein is 5%~15%, the concentration of acrylamide is 2.5%~7.5%; the temperature range of acrylamide molecules inducing silk protein film formation is 10~40 o C; The humidity range for acrylamide molecules to induce silk fibroin film formation is 10%~50%.
[0013] Beneficial effects of the present invention:
[0014] The present invention provides a silk fibroin contact lens prepared based on natural silk. The silk fibroin solution is a solution obtained by mixing natural silk with a lithium bromide solution after degumming. The preparation method is as follows: after removing obvious impurities from the silk, put it into an alkaline solution to degumming, and then mix and stir with the lithium bromide solution to break the hydrogen bond network in the silk fiber, so as to dissolve the silk fibroin into a silk fibroin solution and reprocess it, and then remove the lithium bromide and other soluble impurities contained in the silk fibroin solution by a dialysis method. The silk fibroin lens is a silk fibroin membrane induced by acrylamide molecules, and the preparation method is as follows: acrylamide molecules are blended with a silk fibroin solution to induce amorphous silk fibroin to form a crystalline and stable silk fibroin membrane, and then acrylamide is removed by repeated immersion and rinsing. The silk fibroin lens gives the contact lens a high oxygen permeability function.
[0015] The main ingredients and functions of the silk fibroin contact lenses provided by the present invention are as follows:
[0016] Silk: A natural fiber, silk has a long history of being used to make silk threads, woven ribbons and simple silk fabrics. As a natural source, silk has the function of skin metabolism, can effectively resist mites and inhibit bacteria, does not cause skin allergies, and is often used in the field of beauty. Silk is rich in high-molecular fibroin, accounting for about 70% to 80% of silk. It can be used as a natural source of fibroin.
[0017] Silk fibroin: An excellent natural polymer fibrous protein that has been widely used in the field of biomedical materials. As a natural biomaterial, silk fibroin has many remarkable excellent properties, such as good biocompatibility, suitable mechanical properties, low immunity and controllable degradation rate. In addition, silk fibroin can achieve a multi-scale structure from water-soluble to water-insoluble during the forming process. At present, silk fibroin scaffolds have been widely used in a variety of tissue engineering, including the repair of cartilage, nerves, blood vessels, etc. In addition, its reliable source and easy access are also a major advantage of its wide application.
[0018] Lithium bromide: An inorganic substance that is highly soluble in water. It is a highly efficient water vapor absorbent and air humidity regulator that can be used as an absorption refrigerant, a hydrogen chloride remover in organic chemistry, and a fiber bulking agent. It can break the hydrogen bond network in silk fibers, thereby dissolving silk fibroin into a silk fibroin solution and reprocessing it.
[0019] Acrylamide: used as raw material for organic synthesis and raw material for polymer materials. Usually soluble in water, especially has a good effect on the flocculation of protein and starch in water. It has excellent properties such as flocculation, thickening, shear resistance, drag reduction, and dispersibility.
[0020] In summary, the silk fibroin contact lens provided by the present invention has at least the following beneficial effects:
[0021] High security :The silk protein used to make the lenses comes from nature, has good biocompatibility and is safe for the human body. The porous structure of the lenses makes them highly oxygen permeable, which can prevent eye redness and pain caused by corneal hypoxia and protect the wearer's eye health.
[0022] Environmental friendly :The silk protein that makes up the lens can be degraded in the natural environment and does not pollute the environment.
[0023] The invention solves the technical problems that the existing contact lenses have poor air permeability, and long-term wearing may cause corneal hypoxia, redness, swelling, pain, dryness and even decreased vision in the eyes. BRIEF DESCRIPTION OF THE DRAWINGS
[0024] Figure 1 is a flow chart of the preparation of silk fibroin contact lenses in one embodiment of the present invention;
[0025] Figure 2 It is a schematic diagram of a longitudinal section of a silk fibroin contact lens in one embodiment of the present invention. DETAILED DESCRIPTION
[0026] The present invention adopts the following technical scheme: natural silk is degummed by potassium hydroxide solution and then dissolved by lithium bromide to obtain a silk protein solution; the silk protein solution is dialyzed, mixed with acrylamide, and then dripped into a contact lens mold, and air-dried to obtain a lens.
[0027] like Figure 1 As shown, the preparation process includes the following main steps: natural silk is degummed and then dissolved in lithium bromide to obtain a silk protein solution; the silk protein solution is dialyzed and cleaned, then mixed with acrylamide and dripped into a contact lens mold, and air-dried to obtain a lens.
[0028] Preferably, the lens is a reaction product of lithium bromide, acrylamide and silk fibroin.
[0029] Preferably, the silk protein solution is dialyzed in sterile ultrapure water for 4 to 6 days, and the sterile ultrapure water is replaced every 6 to 24 hours.
[0030] Preferably, the contact lens mold is washed and dried with sterile water and then a mixed solution of acrylamide and silk fibroin is added; the air-drying process temperature is 10-40 o C.
[0031] Preferably, the potassium hydroxide solution is 0.3%~3% (w / v), the concentration of the lithium bromide solution is 1%~10% (g / mL), and the concentration of the acrylamide solution is 2.5%~7.5% (g / mL); the acrylamide solution and the silk protein solution are mixed in a volume ratio of 1:10.
[0032] In some embodiments, in the reaction of acrylamide molecules inducing silk fibroin to form a film, the silk fibroin concentration is 5% to 15%, and the acrylamide concentration is 2.5% to 7.5%. In some embodiments, the temperature range of acrylamide molecules inducing silk fibroin to form a film is 10 to 40 o C. In some embodiments, the humidity range at which the acrylamide molecules induce silk fibroin to form a film is 10% to 50%.
[0033] Main reagents :
[0034] Lithium bromide was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., item number L108934-100g. Acrylamide was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., item number A108465-100g. Potassium hydroxide solution was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., item number P294848-500ml. Silk was purchased from Haozhou Hongbaotang Trading Co., Ltd., item number 18756786088.
[0035] Solution preparation :
[0036] 0.3% (w / v) potassium hydroxide solution: Take 1 mL of 3% (w / v) potassium hydroxide solution and dilute to 10 mL with ultrapure water to obtain 0.3% (w / v) potassium hydroxide solution.
[0037] 1% (g / mL) lithium bromide solution: Weigh 0.1 g of lithium bromide solid particles and add them into 10 mL of ultrapure water to prepare a 1% (g / mL) acrylamide solution.
[0038] 2.5% (g / mL) acrylamide solution: Weigh 0.25 g of acrylamide solid particles and add them into 10 mL of ultrapure water to prepare a 2.5% (g / mL) acrylamide solution.
[0039] If not otherwise specified, the reagents used in the following examples are all conventional reagents in the art, which can be obtained commercially or prepared according to conventional methods in the art, and the specifications are laboratory pure. If not otherwise specified, the experimental methods and conditions used in the following examples are all conventional experimental methods and conditions in the art, and reference can be made to relevant experimental manuals, known documents or manufacturer specifications. Unless otherwise defined, the meanings of all technical and scientific terms used herein are the same as those generally understood by those of ordinary skill in the art to which the present invention belongs.
[0040] Example 1: Preparation of silk fibroin contact lenses from natural silk
[0041] 1. Preparation of silk protein solution
[0042] After removing obvious impurities from the silk, the silk was placed in a 0.5% (w / v) potassium hydroxide solution and boiled for half an hour to remove the glue. The silk was then mixed with a lithium bromide solution and stirred for 60 minutes. The resulting solution was then placed in sterile ultrapure water for dialyzation for 5 days. The sterile ultrapure water was replaced every 12 hours to obtain a silk protein solution.
[0043] 2. Preparation of silk fibroin lenses
[0044] Take 0.1 mL of 25% (g / mL) acrylamide solution and 1 mL of silk protein solution, add them to a 5 mL centrifuge tube and mix evenly, take 100 μL of the mixed solution and drop it into the contact lens mold, place it in a ventilated place at 10~40℃ to air-dry, and obtain a silk protein lens containing acrylamide molecules; soak the lens in sterile ultrapure water for 60 minutes, then remove the soaking solution, add sterile ultrapure water to wash for 30 minutes, repeat this washing three times, and finally put the cleaned silk protein lens into sterile saline for storage.
[0045] Example 2: Preparation of anthocyanin-dyed circular colored silk fibroin contact lenses
[0046] 1. Preparation of silk protein solution
[0047] After removing obvious impurities from the silk, boil it in 0.8% (w / v) potassium hydroxide solution for half an hour to remove the glue, mix it with lithium bromide solution, stir it for 70 minutes, and then dialyze the resulting solution in sterile ultrapure water for 5 days. Change the sterile ultrapure water every 12 hours to obtain a silk protein solution.
[0048] 2. Preparation of silk fibroin lenses
[0049] Take 0.1 mL of 30% (g / mL) acrylamide solution and 1 mL of silk protein solution, add them to a 5 mL centrifuge tube and mix evenly, take 100 μL of the mixed solution and drop it into the contact lens mold, place it in a ventilated place at 10~40℃ to air dry, and obtain a silk protein lens containing acrylamide molecules; soak the lens in sterile ultrapure water for 80 minutes, then remove the soaking solution, add sterile ultrapure water to wash for 30 minutes, repeat this washing three times, and finally put the cleaned silk protein lens into sterile saline for storage.
Claims
1. A method for preparing silk fibroin contact lenses based on natural silk, characterized in that: The method comprises: degumming natural silk with a potassium hydroxide solution and then dissolving the silk with a lithium bromide solution to obtain a silk protein solution; The silk protein solution is dialyzed and mixed with the acrylamide solution, and then dripped into a contact lens mold, and air-dried to obtain a lens; The potassium hydroxide solution is 0.3%~3% w / v, the concentration of the lithium bromide solution is 1%~10% g / mL, and the concentration of the acrylamide solution is 2.5%~7.5% g / mL; the acrylamide solution and the silk protein solution are mixed at a volume ratio of 1:10; the lens is a silk protein film induced by acrylamide molecules, and the preparation method is: blending acrylamide molecules with the silk protein solution to induce the amorphous silk protein to form a crystalline and stable silk protein film, and then removing the acrylamide by repeated soaking and washing; the silk protein concentration is 5%~15%, and the acrylamide concentration is 2.5%~7.5%; the temperature range for acrylamide molecules to induce silk protein film formation is 10~40 o C; The humidity range for acrylamide molecules to induce silk fibroin film formation is 10%~50%.
2. The method for preparing silk fibroin contact lenses based on natural silk according to claim 1, characterized in that: The lens is the reaction product of lithium bromide, acrylamide, and silk fibroin.
3. The method for preparing silk fibroin contact lenses based on natural silk according to claim 1, characterized in that: The silk protein solution was dialyzed in sterile ultrapure water for 4 to 6 days, and the sterile ultrapure water was replaced every 6 to 24 hours.
4. The method for preparing silk fibroin contact lenses based on natural silk according to claim 1, characterized in that: The contact lens mold is washed and dried with sterile water, and then a mixed solution of acrylamide and silk fibroin is added; the air-drying process temperature is 10-40 o C.
5. The method for preparing silk fibroin contact lenses based on natural silk according to claim 1, characterized in that: The silk protein solution is a solution obtained by mixing natural silk with a lithium bromide solution after degumming. The preparation method is as follows: after the impurities are picked out of the silk, the silk is placed in an alkaline solution for degumming, and then mixed with the lithium bromide solution and stirred to break the hydrogen bond network in the silk fiber, thereby dissolving the silk fibroin into a silk protein solution and reprocessing it, and then removing the lithium bromide and soluble impurities contained in the silk protein solution through a dialysis method.