Continuous wave light source non-contact optical tomography system and scanning method

Abstract

The present invention relates to a continuous wave light source non-contact optical tomography system and scanning method, which utilizes a set of hardware systems to simultaneously realize surface information, DOT, FMT and BLT data acquisition, and realize laser scanning at any angle. The system includes a light source module, a scanning module, an imaging module and a control module containing a processor. The light source module includes a laser, a coupler, an optical fiber, a collimator and a focusing lens in sequence, which projects the laser shaping into a scanning galvanometer. The scanning module includes a scanning galvanometer, a slide rail, a rotating stage and a reflector. The scanning module completes the switching between line scanning and point scanning and collects surface information and tomography data. The slide rail completes the switching between transmission and reflection scanning to collect DOT, FMT and BLT data, and the rotating stage realizes illumination at any angle. The system has a simple structure and low cost, and can be applied to macro-scale three-dimensional imaging of organisms with various contrasts, serving the fields of new drug development, disease research, surgical image navigation, agricultural detection, etc.

Description

Technical Field

[0001] The present invention relates to an optical imaging technology, and in particular to a continuous wave light source non-contact optical tomography system and a scanning method. Background Art

[0002] Optical imaging offers numerous advantages, including non-invasiveness, high sensitivity, lack of ionizing radiation, and low cost. It is widely used in areas such as new drug development, disease research, and surgical image navigation. Fluorescent molecular tomography (FMT), diffuse optical tomography (DOT), and bioluminescence tomography (BLT) are currently emerging macroscopic optical molecular imaging techniques. Compared to two-dimensional optical imaging methods, these methods can acquire three-dimensional optical properties of biological tissues or the distribution of fluorescent molecules by establishing mathematical models of diffuse light propagation.

[0003] Currently, DOT technology is primarily used in brain imaging and breast imaging. For breast imaging, when cancerous tissue becomes vascularized, the area becomes hypervascular and hypoxic due to the high concentration of blood vessels and elevated cellular metabolism. This results in stronger light absorption compared to normal tissue. Therefore, DOT technology can utilize imaging of endogenous substances within the breast, such as hemoglobin, water, and lipids, to identify differences in physiological parameters between tumor and normal tissue.

[0004] FMT is used to capture the distribution of fluorescence generated by specific fluorescent material molecules within biological tissues. The process involves implanting a tumor and a targeted fluorescent agent in a small animal. A laser is then used to scan a specific plane within the animal's area. The fluorescent agent is excited by the laser and emits near-infrared light. A detector then captures an image of the excitation light. Finally, a 3D reconstruction is performed to determine the tumor's location and distribution within the animal.

[0005] BLT uses luciferase genes and fluorescent reporter groups, such as green fluorescent protein and red fluorescent protein, to mark cells or DNA. It uses sensitive optical detection instruments to observe biological processes such as the occurrence and development of diseases, tumor growth and metastasis, gene expression and response in living animals, thereby monitoring cell activity and gene behavior in living organisms.

[0006] The basic structure of an optical tomography system mainly consists of a light source, a scanning galvanometer, a stage, and a signal receiver. Existing optical tomography systems are divided into three modes according to the nature of the light source: time domain (TD), frequency domain (FD), and continuous wave (CW); according to the relative position of the light source and the detector, they are divided into transmission and reflection types; according to their scanning method, they are divided into point scanning, line scanning, and surface scanning; according to the design of the illumination light path, they are divided into contact type (mainly using optical fiber) and non-contact type (mainly using spatial free light).

[0007] Most commercial or laboratory-developed optical tomography systems currently have the following common shortcomings:

[0008] 1. Some commercial devices, such as Perkin Elmer's IVIS system, use a mechanical structure connected to optical fiber for illumination. The interval and scanning position of the scanning points are relatively fixed, and the number of scanning points is limited. This makes it difficult to achieve effective 3D reconstruction of complex and delicate targets.

[0009] 2. Most optical tomography systems lack the ability to extract surface information and can only perform three-dimensional tomographic reconstruction on objects with simple geometric shapes (such as cubes, cylinders, etc.). For objects with complex surfaces, their surfaces can only be approximated using regular geometric shapes, which reduces the accuracy of tomographic reconstruction.

[0010] 3. Other systems acquire surface information by integrating multimodal imaging, such as MRI, CT, and ultrasound. Furthermore, some systems utilize surface information extraction modules independent of their optical tomography capabilities (e.g., by installing spatial light modulators, binocular cameras, or depth cameras). Both approaches increase system complexity and cost, and introduce new challenges such as image registration.

[0011] 4. The system designs of the three imaging modalities of DOT, FMT, and BLT are diverse, and a single system is often unable to meet the needs of multiple imaging modalities. Summary of the Invention

[0012] To address the challenges of current optical tomography systems, a non-contact optical tomography system and scanning method using a continuous-wave light source has been proposed. This system can acquire high-precision three-dimensional surface information, integrating three imaging modalities: DOT, FMT, and BLT, and enabling flexible switching between reflection and transmission. This system is low-cost, offers high imaging accuracy, and can capture a wide range of biological information, making it promising for industrialization.

[0013] The technical solution of the present invention is: a continuous wave light source non-contact optical tomography system, including a light source module, a scanning module, an imaging module and a control module containing a processor; the scanning module includes a scanning galvanometer, an optical path switching unit, a transmission optical path adjustment unit and a reflection optical path adjustment unit; the continuous wave light beam output by the light source module after shaping is incident on the scanning galvanometer, and the scanning galvanometer emits scanning light in line scanning and point scanning according to the control signal output by the control module. After the scanning light passes through the optical path switching unit to achieve transmission and reflection switching, it enters the transmission optical path adjustment unit or the reflection optical path adjustment unit to adjust the scanning angle of the incident light. The transmission scanning light directly enters the imaging module to achieve scanning and acquisition, and the reflection scanning light passes through the reflector to enter the imaging module to achieve scanning and acquisition, and the scanning and acquisition data is sent to the processor.

[0014] Preferably, the transmitted light path adjustment unit includes a first 360-degree rotating stage loaded with a first reflector and a first rotating stage support device, the first 360-degree rotating stage is fixed on the first rotating stage support device, the first rotating stage support device is fixed on a slide rail serving as an optical path switching unit, the scanning galvanometer outputs scanning light which is reflected by the first reflector and projected onto the imaging module; the first 360-degree rotating stage includes a displacement disk and an angle disk, the angle disk is fixed on the displacement disk, the angle disk moves with the displacement disk, the reflector is loaded in the middle of the angle displacement disk, there are scale values ​​on the angle displacement disk, and the first 360-degree rotating stage is used to adjust the position state of the first reflector.

[0015] Preferably, the reflected light path adjustment unit includes a second 360-degree rotating stage loaded with a second reflector, a second rotate stage support device and a third reflector. The second 360-degree rotate stage is fixed on the second rotate stage support device. The structure of the second 360-degree rotate stage is the same as the first 360-degree rotate stage. The scanning galvanometer outputs the scanning light which is reflected by the second reflector and the third reflector in turn and projected onto the imaging module.

[0016] Preferably, the transmissive or reflective scanning light is adjusted by a 360-degree rotating stage equipped with a corresponding reflector so that the scanning light is irradiated onto the imaging stage in the imaging module at any angle.

[0017] Preferably, the light source module includes a laser, a coupler, an optical fiber, a collimator and a focusing lens. The laser emits laser light which is coupled into one end of the optical fiber through the coupler, and then input into the collimator from the other end of the optical fiber for collimation and then focused by the focusing lens, so that the light beam is shaped into a beam with a diameter less than or equal to 1 mm and enters the scanning module.

[0018] Preferably, the imaging module includes an imaging stage, a filter wheel and a CMOS / CCD imaging detector. The CMOS / CCD camera is placed directly above the imaging stage and is used to capture line scan images and serve as a detector for tomographic reconstruction data acquisition. Bandpass filters with different central wavelengths are placed in the filter wheel placed between the CMOS / CCD camera and the imaging stage, and the filter wheel is used to switch between different wavelength filters required for data acquisition.

[0019] A continuous wave light source non-contact optical tomography method specifically comprises the following steps:

[0020] 1) Build a continuous wave light source non-contact optical tomography system;

[0021] 2) Set the scanning mode of the scanning galvanometer to point scanning mode, turn on the laser, and the laser is shaped by the light source module into a beam with a diameter less than or equal to 1 mm, which enters the scanning galvanometer;

[0022] 3) Then use the scanning galvanometer to mark the calibration plate, collect the calibration images of the CMOS / CCD camera and the scanning galvanometer, find the laser center point on the calibration image, and calculate the transformation matrix T between the camera coordinate system and the galvanometer coordinate system. cg ;

[0023] 4) Collect the white light image of the sample to be tested and set the actual required point scanning range, extract the calibration points on the sample stage, and calculate the transformation matrix T between the sample coordinate system and the camera coordinate system pc , and use T pc and T cg Convert the actual required scanning points into galvanometer scanning coordinates;

[0024] 5) Perform a pre-scan to verify whether the marking is correct, and then start the formal scan;

[0025] 6) After the scan is complete, turn off the laser and take a picture of the ambient light.

[0026] Furthermore, the step 5) formal scanning is DOT raw data acquisition: the scanning galvanometer scanning mode is set to point scanning mode, the laser is turned on, the laser is shaped into a beam with a diameter less than or equal to 1 mm by the light source module, and enters the scanning galvanometer, the transmission and reflection scanning modes are switched by the optical path switching unit, and the control module is used to control the scanning galvanometer to enter the automatic marking process, while the CMOS / CCD camera obtains the scanned image.

[0027] Furthermore, the step 5) formal scanning is for FMT or BLT raw data acquisition: the scanning galvanometer scanning mode is set to point scanning mode, the laser is turned on, the laser is shaped into a beam with a diameter less than or equal to 1 mm by the light source module, and enters the scanning galvanometer, the transmission and reflection scanning modes are switched by the optical path switching unit, the filter wavelength in the filter wheel is adjusted to collect fluorescence, the scanning galvanometer is controlled by a computer to enter the automatic marking process, and the scanning image is collected by the CMOS / CCD camera at the same time.

[0028] Furthermore, the step 5) is formally scanned to extract surface information: the scanning mode of the scanning galvanometer is set to the line scanning mode, the laser is turned on, the laser is shaped into a beam with a diameter less than or equal to 1 mm by the light source module, and enters the scanning galvanometer. The mobile optical path switching unit is switched to the reflection scanning mode, and the scanning galvanometer is controlled to perform a line scan on the sample surface. At the same time, the CMOS / CCD camera takes a line scan picture, and the light plane equation corresponding to the line scan is used to calculate the three-dimensional point cloud of the sample surface. After the point cloud at the bottom of the object is completed by interpolation, complete three-dimensional surface information is generated for tomographic reconstruction.

[0029] The beneficial effects of the present invention include: the non-contact optical tomography system and scanning method using a continuous wave light source can rapidly switch between transmission and reflection modes, and between point and line scanning modes; the system has a simple structure and low cost, and utilizes a single hardware system to simultaneously acquire surface information, DOT, FMT, and BLT data; laser scanning at any angle is possible; the use of a galvanometer for scanning allows for high repeatability and prevents damage to system components; and the entire acquisition process is controlled by a user-friendly graphical interface, making operation simple. The system can be applied to macroscopic 3D imaging of organisms at various contrast levels, serving applications in new drug development, disease research, surgical image navigation, agriculture, and other fields. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] Figure 1 This is a schematic structural diagram of a continuous wave light source non-contact optical tomography system according to the present invention;

[0031] Figure 2 This is a flow chart of the system acquisition of the present invention;

[0032] Figure 3 This is a flow chart of the automated marking collection process of the system of the present invention;

[0033] Figure 4 This is the flow chart of DOT raw data collection of the system of the present invention;

[0034] Figure 5 This is a flow chart of the FMT raw data acquisition system of the present invention;

[0035] Figure 6This is a flow chart of BLT raw data collection for the system of the present invention;

[0036] Figure 7 This is a flow chart of surface information collection and reconstruction of the system of the present invention;

[0037] Figure 8 This is a schematic diagram of a 360-degree rotating platform of the system of the present invention;

[0038] Figure 9 This is a side view of the 360-degree rotating platform of the system of the present invention;

[0039] Figure 10 This is a schematic diagram of the instrument control circuit of the system of the present invention. DETAILED DESCRIPTION

[0040] The present invention is described in detail below with reference to the accompanying drawings and specific embodiments. This embodiment is implemented based on the technical solution of the present invention, and provides a detailed implementation method and specific operation process, but the protection scope of the present invention is not limited to the following embodiments.

[0041] like Figure 1 The structure diagram of the continuous wave light source non-contact optical tomography system shown is as follows, the system includes a light source module, a scanning module, an imaging module and a control module including a processor; the light source module includes a laser 201, a coupler 202, an optical fiber 203, a collimator 204 and a focusing lens 205; the scanning module includes a scanning galvanometer 1, a slide rail 2, a 360-degree rotating stage (4, 8), a rotating stage support device (5, 6) and three reflecting mirrors (3, 7, 9); the imaging module includes an imaging stage 101, a filter wheel 102 and a CMOS / CCD imaging detector 103.

[0042] The laser 201 in the light source module emits laser light, which is coupled into one end of the optical fiber 203 through the coupler 202. The laser light is then input into the collimator 204 from the other end of the optical fiber 203 for collimation and then focused by the focusing lens 204, so that the beam is shaped into a beam with a diameter less than or equal to 1 mm and enters the scanning module.

[0043] The scanning module forms a scanning light from the shaped laser beam to scan the sample being measured on the imaging stage 101. The scanning mode can be divided into line scanning and point scanning. Line scanning is used to extract sample surface information; point scanning is used to collect tomographic reconstruction data. The scanning mode setting can be divided into transmission and reflection. The transmission and reflection acquisition modes can be switched using the optical path switching unit to achieve transmission or reflection scanning mode to obtain DOT, FMT and BLT scanning data; the reflectors 3 and 7 are both connected to their respective 360-degree rotating stages to achieve scanning of incident light at any angle, and finally incident on the imaging stage 101 to scan the sample being measured. Figure 2 The system acquisition flow chart is shown.

[0044] The scanning module includes an optical path switching unit, a transmission optical path adjustment unit, and a reflection optical path adjustment unit. The optical path switching unit includes a slide rail 2; the transmission optical path adjustment unit includes a reflector 3, a 360-degree rotating stage 4, and a rotating stage support device 5; and the reflection optical path adjustment unit includes the rotating stage support device 5, a reflector 7, a 360-degree rotating stage 8, and a reflector 9. The reflector 3 is fixed to the 360-degree rotating stage 4, which is fixed to the rotating stage support device 5, which is fixed to the slide rail 2. Adjusting the incident angle of the reflector 3 by adjusting the 360-degree rotating stage 4 adjusts the incident angle of the transmitted light. Switching between the reflection and transmission optical paths is achieved by moving the slide rail 2. When the optical path is in transmission mode, the light beam exits scanning galvanometer 1 and is directly reflected by reflector 3 and projected onto the stage. When switched to reflection mode, reflector 3, turntable 4, and turntable support 5 are moved away by slide rail 2. The light beam exits scanning galvanometer 1 and is reflected by reflectors 7 and 9 and projected onto the stage. Reflector 7 is fixed to 360-degree turntable 8, which is fixed to turntable support 6. By adjusting the incident angle of reflector 7 through 360-degree turntable 8, the incident angle of the reflected light can be adjusted.

[0045] The CMOS / CCD camera 103 is placed directly above the imaging stage 101 . The CMOS / CCD camera 103 is used to capture line scan images and serve as a detector for tomographic reconstruction data acquisition.

[0046] The filter wheel 102 placed between the CMOS / CCD camera 103 and the imaging stage 101 is used to switch between filters of different wavelengths required for data acquisition.

[0047] The automated marking acquisition process is as follows: First, set the scanning mode of the scanning galvanometer 1 to point scanning mode and turn on the laser 201. The laser is shaped into a beam with a diameter of 1mm by the light source module and enters the scanning galvanometer 1. Then, use the scanning galvanometer 1 to mark the calibration plate and collect the calibration images of the CMOS / CCD camera 103 and the scanning galvanometer 1. Then find the center point of the laser on the calibration image and calculate the transformation matrix T between the camera coordinate system and the galvanometer coordinate system. cg Next, collect the white light image of the sample to be tested and set the actual required point scanning range. Then, extract the calibration points on the sample stage and calculate the transformation matrix T between the sample coordinate system and the camera coordinate system. pc , and use T pc and T cgThe actual required scanning points are converted into galvanometer scanning coordinates. Then a pre-scan is performed to verify whether the marking is correct. If the marking does not meet the actual requirements, the operation is stopped and the actual required scanning point range is reset. Then the automatic pre-marking process is entered again. If it is correct, the formal scan is started. Finally, after the scan is completed, the laser is turned off and an ambient light picture is taken. The flow chart is as follows Figure 3 shown.

[0048] The DOT raw data acquisition process is as follows: set the scanning mode of the scanning galvanometer 1 to the point scanning mode and turn on the laser 201. The laser is shaped into a beam with a diameter less than or equal to 1mm by the light source module and enters the scanning galvanometer 1. The transmission and reflection scanning modes can be switched using the optical path switching unit. The computer is used to control the scanning galvanometer 1 to enter the automatic marking process, and the CMOS / CCD camera 103 obtains the scanned image. The flow chart is as follows Figure 4 shown.

[0049] The FMT raw data acquisition process is as follows: set the scanning mode of the scanning galvanometer 1 to point scanning mode and turn on the laser 201. The laser is shaped into a beam with a diameter of 1mm by the light source module and enters the galvanometer. The transmission and reflection scanning modes can be switched by using the mobile optical path switching unit, and the wavelength of the filter in the filter wheel 102 is adjusted to collect fluorescence. The computer is used to control the scanning galvanometer 1 to enter the automatic marking process, and the CMOS / CCD camera 103 collects the scanned image at the same time. The flow chart is as follows Figure 5 shown.

[0050] The BLT raw data acquisition process is as follows: set the scanning galvanometer scanning mode to point scanning mode and turn on the laser. The laser is shaped into a beam with a diameter of 1mm by the light source module and enters the scanning galvanometer 1. The transmission and reflection scanning modes can be switched by using the mobile optical path switching unit, and the wavelength of the filter in the filter wheel 102 is adjusted to collect spontaneous fluorescence. The computer is used to control the scanning galvanometer 1 to enter the automatic marking process, and the CMOS / CCD camera 103 collects the scanned image at the same time. The flow chart is as follows Figure 6 shown.

[0051] The process of extracting surface information is as follows: first, set the scanning mode of the scanning galvanometer 1 to the line scanning mode, and turn on the laser 201. The laser is shaped into a beam with a diameter of 1 mm by the light source module and enters the scanning galvanometer 1. Move the optical path switching unit, switch to the reflection scanning mode, control the scanning galvanometer 1 to perform a line scan on the sample surface, and at the same time, the CMOS / CCD camera 103 takes a line scan picture. The three-dimensional point cloud of the sample surface is calculated using the light plane equation corresponding to the line scan, and the complete three-dimensional surface information is generated after the point cloud at the bottom of the object is completed by interpolation for tomographic reconstruction. The flow chart is as follows Figure 7 shown.

[0052] In one embodiment, the scanning module performs two modes of scanning. The purpose of the optical path switching unit is to act as a switch to switch between the transmission mode and the reflection mode at any time. The optical path of the transmission mode is: the light is emitted from the scanning galvanometer 1, passes through the reflector 3, and is finally emitted to the imaging target on the imaging stage 101. When using the reflection mode, the reflector 3 is removed by the slide rail switching device 2. The reflective optical path is: the light is emitted from the scanning galvanometer 1, passes through the reflectors 7 and 9, and is finally emitted to the imaging target on the imaging stage 101.

[0053] In one embodiment, the imaging module comprises an imaging stage 101 for placing the sample for examination. A filter wheel 102, which contains bandpass filters with different center wavelengths, is positioned between the CMOS / CCD detector 103 and the imaging target. The filter wheel 102 filters out the excitation light from the laser while allowing fluorescence to pass through and enter the imaging detector 103.

[0054] In one embodiment, the multi-angle scanning module is as follows: the reflectors 3 and 7 are connected to the 360-degree rotating stages 4 and 8 respectively, the displacement plate is fixed vertically on the 90-degree right-angle bracket 401, the angle plate 402 is fixed on the displacement plate and can move with the displacement plate, and the reflector 3 is mounted in the middle of the angle plate 402. There are scale values ​​on the angle stage 402, and the scale can be adjusted to make the scanning light irradiate the imaging stage 101 at any angle. Figure 8 、 9 shown.

[0055] In one embodiment, surface information extraction and DOT, FMT, and BLT image acquisition are performed as follows: After the shaped laser beam from the light source area enters the galvanometer, two scanning modes can be set: point scanning and line scanning. When the reflective line scanning mode is selected, sample surface information extraction can be performed. After surface information extraction, DOT, FMT, and BLT scan data can be acquired by switching the optical path switching unit and the scanning mode of the galvanometer 1 to achieve either transmission or reflection point scanning mode.

[0056] In one embodiment, the processor is a computer as the control layer, and the camera, filter wheel, scanning galvanometer and laser are field layer devices. Specifically, the computer controls the opening and closing of the laser, the scanning mode (point scanning or line scanning) of the galvanometer, the number of scanning points, the conversion of the filter position inside the filter wheel, and the camera shooting and image storage. All communication interfaces are connected by USB data cables. The control diagram is shown in FIG. Figure 10 shown.

[0057] The above-described embodiments merely illustrate several implementations of the present invention, and while their descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the patent. It should be noted that a person skilled in the art would be able to make numerous variations and improvements without departing from the spirit of the present invention, all of which fall within the scope of protection of the present invention. Therefore, the scope of protection of the patent for this invention shall be determined by the appended claims.

Claims

1. A continuous wave light source non-contact optical tomography system, characterized in that: The system comprises a light source module, a scanning module, an imaging module and a control module including a processor; the scanning module comprises a scanning galvanometer, an optical path switching unit, a transmission optical path adjustment unit and a reflection optical path adjustment unit; the continuous wave light beam after being outputted by the light source module is incident on the scanning galvanometer, and the scanning galvanometer emits scanning light in line scanning and point scanning according to the control signal outputted by the control module; the scanning light is switched between transmission mode and reflection mode by the optical path switching unit, and then enters the transmission optical path adjustment unit or the reflection optical path adjustment unit to adjust the scanning angle of the incident light; the transmission scanning light is directly incident on the imaging module to realize scanning and acquisition; the reflection scanning light is incident on the imaging module through the reflector to realize scanning and acquisition, and the scanning and acquisition data is sent to the processor; The transmission light path adjustment unit includes a first 360-degree rotating stage with a first reflector mounted thereon and a first rotating stage support device, the first 360-degree rotating stage being fixed to the first rotating stage support device, and the first rotating stage support device being fixed to a slide rail serving as an optical path switching unit, the scanning galvanometer outputs scanning light which is reflected by the first reflector and projected onto the imaging module; the first 360-degree rotating stage includes a displacement disk and an angle disk, the angle disk being fixed to the displacement disk and moving with the displacement disk, the reflector being mounted in the middle of the angle displacement disk, and having scale values ​​on the angle displacement disk, and the first 360-degree rotating stage being used to adjust the position state of the first reflector; The reflected light path adjustment unit includes a second 360-degree rotating stage with a second reflector, a second rotated stage support device and a third reflector. The second 360-degree rotated stage is fixed on the second rotated stage support device. The structure of the second 360-degree rotated stage is the same as the first 360-degree rotated stage. The scanning galvanometer outputs the scanning light which is reflected by the second reflector and the third reflector in turn and projected onto the imaging module.

2. The continuous wave light source non-contact optical tomography system according to claim 1, characterized in that: The transmissive or reflective scanning light is adjusted by a 360-degree rotating stage equipped with a corresponding reflector so that the scanning light is irradiated onto the imaging stage in the imaging module at any angle.

3. The continuous wave light source non-contact optical tomography system according to claim 1 or 2, characterized in that: The light source module includes a laser, a coupler, an optical fiber, a collimator and a focusing lens. The laser emits laser light, which is coupled into one end of the optical fiber through the coupler, and then input into the collimator from the other end of the optical fiber for collimation and then focused by the focusing lens, so that the light beam is shaped into a beam with a diameter less than or equal to 1 mm and enters the scanning module.

4. The continuous wave light source non-contact optical tomography system according to claim 1 or 2, characterized in that: The imaging module includes an imaging stage, a filter wheel, and a CMOS / CCD imaging detector. The CMOS / CCD camera is placed directly above the imaging stage and is used to capture line scan images and serve as a detector for tomographic reconstruction data acquisition. The filter wheel, placed between the CMOS / CCD camera and the imaging stage, is used to place bandpass filters with different central wavelengths. The filter wheel is used to switch between filters of different wavelengths required for data acquisition.

5. A continuous wave light source non-contact optical tomography method, characterized in that: The specific steps include: 1) Building the continuous wave light source non-contact optical tomography system according to claim 3; 2) Set the scanning mode of the scanning galvanometer to point scanning mode, turn on the laser, and the laser is shaped by the light source module into a beam with a diameter less than or equal to 1mm, which enters the scanning galvanometer; 3) Then use the scanning galvanometer to mark the calibration plate, collect the calibration images of the CMOS / CCD camera and the scanning galvanometer, find the laser center point on the calibration image, and calculate the transformation matrix T between the camera coordinate system and the galvanometer coordinate system cg ; 4) Collect the white light image of the sample to be tested and set the actual required point scanning range, extract the calibration points on the sample stage, and calculate the transformation matrix T between the sample coordinate system and the camera coordinate system pc , and use T pc and T cg Convert the actual required scanning points into galvanometer scanning coordinates; 5) Perform a pre-scan to verify whether the marking is correct, and then start the formal scan; 6) After the scan is complete, turn off the laser and take a picture of the ambient light.

6. The continuous wave light source non-contact optical tomography method according to claim 5, characterized in that: Step 5) formal scanning is DOT raw data acquisition: the scanning galvanometer scanning mode is set to point scanning mode, the laser is turned on, and the laser is shaped into a beam with a diameter less than or equal to 1 mm by the light source module and enters the scanning galvanometer. The transmission and reflection scanning modes are switched by the optical path switching unit. The control module controls the scanning galvanometer to enter the automatic marking process, and the CMOS / CCD camera obtains the scanned image.

7. The continuous wave light source non-contact optical tomography method according to claim 5, characterized in that: Step 5) Formal scanning for FMT or BLT raw data acquisition: Set the scanning galvanometer scanning mode to point scanning mode, turn on the laser, and shape the laser into a beam with a diameter less than or equal to 1 mm through the light source module. The laser enters the scanning galvanometer, and the optical path switching unit switches between transmission and reflection scanning modes. The wavelength of the filter in the filter wheel is adjusted to collect fluorescence. The computer controls the scanning galvanometer to enter the automatic marking process, and the CMOS / CCD camera simultaneously captures the scanned image.

8. The continuous wave light source non-contact optical tomography method according to claim 5, characterized in that: The step 5) is a formal scan to extract surface information: the scanning mode of the scanning galvanometer is set to line scanning mode, the laser is turned on, and the laser is shaped into a beam with a diameter less than or equal to 1 mm by the light source module, entering the scanning galvanometer. The mobile optical path switching unit is switched to the reflection scanning mode, and the scanning galvanometer is controlled to perform a line scan on the sample surface. At the same time, the CMOS / CCD camera takes a line scan image, and the light plane equation corresponding to the line scan is used to calculate the three-dimensional point cloud of the sample surface. After the point cloud of the bottom of the object is completed by interpolation, complete three-dimensional surface information is generated for tomographic reconstruction.

Images