A device for real-time measurement of mechanical properties of spherical soft materials and its working method

Abstract

The present invention provides a device for real-time measurement of mechanical properties of spherical soft materials, comprising a fluid driver, a first conduit, an observation device and a collection cup arranged in sequence, wherein a channel with a special structure is arranged in the observation device, the fluid driver is connected to the channel via the first conduit, and the channel is connected to the collection cup via the second conduit, so as to drive the spherical soft material into the channel through the fluid power of the fluid driver, thereby realizing real-time measurement of mechanical properties of the spherical soft material. The present invention has reasonable design, convenient operation, can measure soft materials with changing mechanical properties in real time, can measure soft materials in a liquid environment, has fast measurement speed and accurate measurement results.

Description

Technical Field

[0001] The invention relates to a device for real-time measuring the mechanical properties of spherical soft materials and a working method thereof. Background Art

[0002] In recent years, the development, optimization and application of spherical soft materials have received extensive attention. Spherical hydrogel materials have been widely used in the fields of food industry, biology, medicine, etc. In the application process of spherical soft materials, the mechanical properties of spherical soft materials need to match the mechanical properties of their application environment. For example, the mechanical properties of injectable spherical soft materials need to be similar to those of blood cells; the mechanical properties of edible spherical soft materials need to be similar to those of gastrointestinal tissues; the mechanical properties of spherical soft materials used for tissue culture need to be similar to those of specific tissues.

[0003] However, there is still a lack of a suitable device for real-time quantitative detection of the mechanical properties of spherical soft materials. Existing measurement methods, such as rheometers, texture analyzers and mechanical measuring machines, are difficult to achieve the above functions. The main difficulties are as follows: (1) It is difficult to fix spherical soft materials with low modulus and high deformation capacity during the measurement process; (2) It is necessary to apply a precisely adjustable and relatively gentle force to achieve the loading of spherical soft materials; (3) The application process of spherical soft materials is often accompanied by changes in mechanical properties, such as the deconstruction of soft materials (Zhao, Z, 2021, DOI: 10.1021 / acsnano.1c02147). It is challenging to measure the mechanical properties of spherical soft materials with changing mechanical properties in real time; (4) The application environment of soft materials such as hydrogels is often an aqueous environment. Traditional measurement methods are often unable to achieve the measurement of mechanical properties of soft materials in an aqueous environment, and the measurement accuracy is limited due to the escape of water in the hydrogel material during the force loading process. Summary of the invention

[0004] The present invention improves the above-mentioned problem, that is, the technical problem to be solved by the present invention is to provide a device for real-time measurement of the mechanical properties of spherical soft materials. The device is easy to use and can measure the spherical soft materials with changes in mechanical properties in real time. It can measure spherical soft materials in a liquid environment. The device has a fast measurement speed and accurate measurement results, and solves the problems of complex operation and large measurement errors of existing mechanical properties technologies.

[0005] The specific implementation scheme of the present invention is constructed as follows, and it includes a fluid driver, a first conduit, an observation device and a collection cup which are arranged in sequence. A channel with a special structure is provided in the observation device. The fluid driver is connected to the channel via a first conduit, and the channel is connected to the collection cup via a second conduit, so as to drive the spherical soft material into the channel through the fluid power of the fluid driver, thereby realizing real-time measurement of the mechanical properties of the spherical soft material.

[0006] Furthermore, the fluid driver is a syringe pump, a hydraulic pump, a pneumatic pump and a peristaltic pump.

[0007] Furthermore, the fluid driver is a syringe pump, a syringe is provided at the working end of the syringe pump, and a NaCl solution is provided inside the syringe.

[0008] Furthermore, the channel with a special structure includes one or more of a "T"-shaped channel, a sheath flow channel, a cross-shaped channel, a contraction-shaped channel, and a narrow slit-shaped channel, and the wall of the channel can exert a force on the spherical soft material under the action of fluid loading.

[0009] Furthermore, a first conduit groove and a second conduit groove are respectively provided on both sides of the channel, and the channel is a contraction-type channel, the width of the left side of the contraction-type channel remains unchanged, and the width of the right side of the contraction-type channel decreases successively.

[0010] Furthermore, a working method of a device for real-time measurement of the mechanical properties of spherical soft materials is provided, characterized in that the fluid force of the fluid driver drives the spherical soft material into a channel with a special structure, and the deformation information and flow field information of the spherical soft material in the channel are obtained through numerical simulation analysis to determine the mechanical properties of the spherical soft material.

[0011] Furthermore, the deformation information includes one or more of the position and deformation of the spherical soft material when it reaches force equilibrium in the channel, the speed and deformation of the spherical soft material moving in the channel, wherein the force equilibrium position of the spherical soft material in the channel and the amount of deformation thereat are preferably measured.

[0012] Furthermore, the flow field information includes channel size, flow field velocity and flow field pressure distribution.

[0013] Furthermore, there are two ways of numerical simulation analysis: (1) Determine the mechanical properties of spherical soft materials based on the deformation information and flow field information of spherical soft materials through fluid-solid coupling simulation; (2) First calculate the fluid force on the spherical hydrogel through fluid mechanics simulation, and then calculate the mechanical properties of the spherical soft material through solid mechanics simulation.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The present invention realizes the real-time and accurate measurement of the mechanical properties of spherical soft materials, which is beneficial to the mechanical measurement of soft materials, material development and optimization, and material application and development;

[0016] (2) The device for real-time measurement of mechanical properties of spherical soft materials of the present invention is conducive to fixing and loading spherical soft materials with low modulus and high deformation capacity, and can realize the measurement of mechanical properties of soft materials with mechanical property changes in a liquid environment, and avoid the limitation of measurement accuracy caused by the escape of water in the soft material during the loading process;

[0017] (3) The measuring method of the present invention has the advantages of simple equipment, simple operation, low measuring cost, fast measuring speed and accurate measuring results, and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a schematic diagram of the structure of Embodiment 1 of the present invention;

[0019] Figure 2 This is a schematic diagram of the protrusion structure of the fluid dynamic loading mold in Example 1 of the present invention;

[0020] Figure 3 This is a schematic diagram of the structure of the fluid power loading mold according to Example 1 of the present invention;

[0021] Figure 4 This is a motion and deformation diagram of the spherical hydrogel in Example 2 of the present invention;

[0022] Figure 5 This is a schematic diagram of the force balance position distance of Example 2 of the present invention;

[0023] Figure 6 The force balance position distance of different spherical hydrogels in the channel contraction area of ​​the fluid dynamic loading device in Example 2 of the present invention;

[0024] Figure 7 This is a SEM image of the spherical hydrogel after vacuum freeze-drying in Example 2 of the present invention.

[0025] Figure 8 A schematic diagram of flow field velocity distribution and pressure distribution of fluid dynamics and finite element simulation in Example 2 of the present invention;

[0026] Fig. 9 A schematic diagram of stress magnitude in a spherical hydrogel simulated by fluid dynamics and finite element method in Example 2 of the present invention;

[0027] Fig.10 It is a strain diagram of fluid dynamics and finite element simulation of Example 2 of the present invention;

[0028] Fig.11 Component diagram of stress and strain of fluid dynamics and finite element simulation in Example 2 of the present invention;

[0029] Fig.12 This is a schematic diagram of the forces acting on the spherical hydrogel by fluid dynamics and finite element simulation in Example 2 of the present invention;

[0030] Fig.13 Schematic diagram of the deformation and microstructure of the spherical hydrogel after deconstruction in Example 3 of the present invention Figure 1; (Equilibrium position of spherical hydrogel in the channel after deconstruction for 10 min at different pH values ​​(4.8, 3.6, 2.4, 1.2))

[0031] Fig.14 Schematic diagram of the deformation and microstructure of the spherical hydrogel after deconstruction in Example 3 of the present invention Figure 2 ; (Equilibrium position of spherical hydrogel in the channel after deconstruction for different time periods (5, 10, 15, 20 min) at pH = 1.2)

[0032] Fig.15 Schematic diagram of the deformation and microstructure of the spherical hydrogel after deconstruction in Example 3 of the present invention Figure 3 ; (SEM images of spherical hydrogel deconstructed at different pH values)

[0033] Fig.16 Schematic diagram of the deformation and microstructure of spherical hydrogel after deconstruction in three examples of the present invention Figure 4 ; (SEM images of spherical hydrogels at different decomposition times at pH = 1.2.)

[0034] In the figure: 1-NaCl solution, 2-syringe, 3-syringe pump, 4-first catheter, 5-optical glass, 6-observation device, 7-spherical hydrogel, 8-contractile channel, 9-beaker to collect NaCl solution, 10-second catheter, 11-fluid power loading mold, 12-protrusion. DETAILED DESCRIPTION

[0035] The present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0036] Example 1: Figure 1-16 As shown, a device for real-time measurement of the mechanical properties of spherical soft materials is provided, comprising a fluid driver, a first conduit 4, an observation device 6 and a collection cup 9 arranged in sequence, wherein a channel with a special structure is provided in the observation device, the fluid driver is connected to the channel via the first conduit 4, and the channel is connected to the collection cup 9 via the second conduit 10.

[0037] The above-mentioned collecting cup can be a beaker for collecting NaCl solution.

[0038] The real-time measurement of the mechanical properties of spherical soft materials is achieved by combining fluid dynamic loading and numerical simulation. The spherical soft material is driven into the channel by the fluid dynamics of the fluid driver and stopped at a certain position in the channel, and the real-time mechanical properties of the spherical soft material are determined by numerical simulation analysis.

[0039] In this embodiment, the fluid driver may be a syringe pump, a hydraulic pump, a pneumatic pump, or a peristaltic pump; the driving form may be setting the flow rate or setting the pressure.

[0040] The fluid driver is a syringe pump 3 , a syringe 2 is provided at the working end of the syringe pump, and a NaCl solution 1 is provided inside the syringe.

[0041] In this embodiment, the channel with a special structure includes one or more of a "T"-shaped channel, a sheath flow channel, a cross-shaped channel, a contraction-shaped channel, and a narrow slit-shaped channel, and the wall of the channel can exert a force on the spherical soft material under the action of fluid loading.

[0042] The material of the above-mentioned channel is one or more of polymer materials, inorganic materials, metal materials, etc. Among them, the channel is preferably formed by combining polydimethylsiloxane (PDMS) and glass.

[0043] The above-mentioned observation device comprises an observation block and an optical glass 5 which are arranged in sequence from top to bottom. The material of the observation block is dimethylsiloxane (PDMS), and a channel is arranged between the observation block and the optical glass.

[0044] In this embodiment, a first conduit groove and a second conduit groove are respectively provided on both sides of the channel. The channel is a contraction type channel 8, the width of the left side of the contraction type channel remains unchanged, and the width of the right side of the contraction type channel decreases successively.

[0045] The width of the input end of the contraction type channel is 4 mm, the width of the output end is 1 mm, and the length of the contraction region on the right side of the contraction type channel is 20 mm.

[0046] The observation block is made of a fluid dynamic loading mold 11, which can be made of 45# steel material. The fluid dynamic loading mold includes a rectangular block and a groove arranged on the rectangular block. The inner bottom surface of the groove is provided with a protrusion 12 that matches the shape of the contraction type channel. The PDMS A liquid: B liquid = 10:1 ratio is mixed and stirred until it turns white, and then vacuumed to remove bubbles, inverted mold, baked at 70°C for 30 minutes, placed at room temperature, demolded, holes are punched at its left and right ends, and then bonded with optical glass, and then baked at 70°C for 60 minutes to form an observation device with a channel.

[0047] In this embodiment, the measurement range of the spherical soft material modulus is 10 -3 ~10 5 Pa, spherical soft materials have lower modulus and greater deformation capacity.

[0048] The mechanical properties of the spherical soft material may not change during the measurement period, or may change during the measurement period. Preferably, the change of the mechanical properties of the soft material is regulated by: changing one or more of the pH value of the liquid in the channel, the content of the degradation enzyme, the temperature, the solvent content, the degradation time, the ultrasound intensity, the irradiation intensity, and the oxidation degree.

[0049] In this embodiment, during operation: the fluid force of the fluid driver drives the spherical soft material into a channel with a special structure, and the deformation information and flow field information of the spherical soft material in the channel are obtained through numerical simulation analysis to determine the mechanical properties of the spherical soft material.

[0050] The above deformation information includes one or more of the position and deformation of the spherical soft material when it reaches force equilibrium in the channel, the speed and deformation of the spherical soft material moving in the channel, among which it is preferred to measure the force equilibrium position of the spherical soft material in the channel and its deformation at that location.

[0051] The above-mentioned flow field information includes channel size, flow field velocity and flow field pressure distribution.

[0052] There are two ways to perform the numerical simulation analysis mentioned above: one is to determine the mechanical properties of the spherical soft material based on the deformation information and flow field information of the spherical soft material through fluid-solid coupling simulation; the other is to first calculate the fluid force on the spherical hydrogel through fluid mechanics simulation, and then calculate the mechanical properties of the spherical soft material through solid mechanics simulation. You can choose any of the above two methods.

[0053] Example 2: Raw materials, instruments and software: konjac glucomannan, sodium alginate, carrageenan, fluid dynamic loading device chip, syringe pump, magnetic stirrer, fluid and solid mechanics simulation analysis software.

[0054] (1) Sodium alginate (SA) of different weights (0.50 g, 0.75 g, 1.00 g, and 1.25 g) were added to 100 mL of distilled water and stirred in a mechanical stirrer at 60 °C and 400 r / min until dissolved (using an electronic constant speed stirrer: JHS-1, Hangzhou Instrument and Motor Co., Ltd.). Then, 0.20 g of carrageenan (KC) was added to the SA solution and stirred until dissolved. After KC was evenly dispersed in the SA solution, 0.15 g of yellow water powder was added to each sol. Finally, 0.10 g of konjac glucomannan (KGM) was added, stirred until fully mixed, cooled to room temperature, and centrifuged to remove bubbles (high-speed centrifuge, TDL-50, Wanfeng Instrument Manufacturing Co., Ltd.). The bubble-free sol was dripped into a 3% CaCl2 solution using a 10 mL syringe through a syringe pump, and the spherical hydrogel was cross-linked with CaCl2 for 20 min. min, and then taken out and rinsed repeatedly with deionized water for 3 times; according to the different sodium alginate concentrations mentioned above, the corresponding spherical hydrogels were coded as KSK-1, KSK-2, KSK-3 and KSK-4 (K:KGM, S:SA, K:KC).

[0055] (2) A spherical hydrogel 7 with a diameter of 3.50 mm was pushed by a 2.5% NaCl solution at a certain flow rate (1.5 mL / min). The spherical hydrogel moved at a certain speed along the direction of the fluid force. When the spherical hydrogel reached the inlet of the device, the timing was started, and photos were taken to record the position of the spherical hydrogel at different times until the spherical hydrogel reached the force balance position of the chip contraction area, and the flow rate was recorded. ImageJ was used to measure the distance from the starting point of the chip contraction area to the spherical hydrogel to the force balance position. SPSS20.0 was used to analyze the statistical data. The t-test was used to detect significant differences. P < 0.05 was statistically significant. Spherical hydrogels with different ratios stopped at different force balance positions. This is because as the SA ratio increases, the hardness increases and the deformation ability decreases. The force balance position of the spherical hydrogel with more SA is closer to the entrance of the contraction channel. The distance between the center of the spherical hydrogel at the force balance position and the entrance of the contraction channel (such as Figure 4 ), as a parameter to indicate the force equilibrium position and deformation of the spherical hydrogel (e.g. Figure 5 ). This distance becomes shorter as the SA ratio increases (e.g. Figure 6 ), the distances of KSK-1 spherical hydrogel, KSK-2 spherical hydrogel, KSK-3 spherical hydrogel and KSK-4 spherical hydrogel were 6.99 ± 0.073 mm, 5.61 ± 0.023 mm, 4.46 ± 0.19 mm and 3.89 ± 0.016 mm, respectively. These data were analyzed by SPSS software, and the results showed that there were significant differences in the distances of the four spherical hydrogels (p < 0.01);

[0056] (3) The cross-sectional microstructure of the spherical hydrogel is shown in Figure 7 As shown in the figure, spherical hydrogels with four compounding ratios were freeze-dried and observed by SEM; after freeze-drying, the spherical hydrogels were honeycomb-shaped with wrinkles and depressions on the surface; from the SEM results, it can be seen that with the increase of SA ratio, the diameter of the cross-linked pores becomes smaller, the number of pores decreases, and the degree of cross-linking increases; when the concentration of SA is 1.25%, the spherical hydrogel forms a dense plane with almost only ice crystal pores and no cross-linked pores; these results show that the prepared spherical hydrogels have different microstructures and may have different strengths. These composite spherical hydrogels provide suitable materials for the development of new measurement methods.

[0057] (4) Based on the results of the fluid dynamic loading experiment, computational fluid dynamics (CFD) and finite element simulation (FEM) simulations were performed to determine the equivalent modulus of the spherical hydrogel. First, CFD simulation was used to calculate the velocity distribution and pressure distribution in the channel containing the spherical hydrogel at the force balance position. The results showed that the flow rate was negatively correlated with the cross-sectional area of ​​the channel. The fluid pressure in front of the spherical hydrogel was greater than the fluid pressure behind the hydrogel. The flow field force acting on the spherical hydrogel at the force balance position was obtained through this pressure difference. (e.g. Figure 8 Then, finite element simulations were used to determine the equivalent modulus of the spherical hydrogel; as the spherical hydrogel moved to the force equilibrium position, the stress and strain in the spherical hydrogel increased (as shown in Fig. 9 , 10 The region with the highest stress and strain is close to the wall of the contraction channel; the stress-strain components show that the front end of the spherical hydrogel is under pressure and the rear end is under tension (as shown in Fig.11 As the spherical hydrogel enters the channel, the force acting on it increases until the hydrogel reaches a force equilibrium position (as shown in Fig.12 as shown); the force depends on the equivalent modulus of the spherical hydrogel set in the FEM model; the equivalent modulus is adjusted until the force acting on the spherical hydrogel calculated by the FEM is equal to the fluid force calculated by the CFD method. At this time, the equivalent modulus set in the FEM is the modulus of the spherical hydrogel obtained by the test method of the present invention.

[0058] In order to evaluate the accuracy of the measurement method of the present invention, a universal tensile measuring machine was used to determine the modulus of the gel material. Since it is difficult for a mechanical testing machine to clamp and measure the mechanical properties of spherical hydrogels, KSK hydrogel cubes were used instead of spherical hydrogels (Table 1 below), and the effectiveness of the measurement method of the present invention was demonstrated by comparing the two measurement methods.

[0059]

[0060] Table 1 Modulus data of four KSK hydrogels measured by two methods

[0061] Example 3: Based on Example 2, in this example, in order to achieve real-time dynamic mechanical measurement of spherical hydrogels, gels were prepared according to the proportions of the KSK-4 spherical hydrogels in Case 1 and deconstructed.

[0062] The deconstruction of spherical hydrogels was carried out by passing different pH solutions (pH = 1.2, 2.4, 3.6, 4.8) through the spherical hydrogels for 10 minutes, and passing pH = 1.2 solution through the spherical hydrogels for different times (5, 10, 15, 20 minutes). As the pH value of the solution decreases, the distance from the force equilibrium position of the spherical hydrogel to the starting point of the chip contraction area after deconstruction treatment increases (e.g. Fig.13As shown). The force equilibrium position distances of the gels treated with pH = 4.8, pH = 3.6, pH = 2.4, and pH = 1.2 were 4.50 ± 0.07 mm, 4.72 ± 0.16 mm, 5.03 ± 0.11 mm, and 5.30 ± 0.09 mm, respectively. When the spherical hydrogels were treated with pH = 1.2 solution for different time periods, the force equilibrium position distance increased with time (as shown). Fig.14 The force equilibrium distances of the gels treated for 5min, 10min, 15min and 20min were 4.66±0.07mm, 5.26±0.15mm, 5.70±0.12mm and 5.77±0.15mm, respectively. The experimental results show that when the spherical hydrogels are in an acidic solution, their mechanical properties decrease, and the degree of decrease increases with the increase of the acidity of the solution and the increase of the deconstruction time.

[0063] The spherical hydrogels after different deconstruction treatments were subjected to liquid nitrogen vacuum freeze drying and then SEM measurements were performed. After deconstruction, the microstructure of the spherical hydrogels changed: the structure was dense before deconstruction, and gaps appeared after deconstruction. With the decrease of pH value and the increase of deconstruction time, the microstructure of the deconstructed spherical hydrogels became looser (e.g. Fig.15 , 16 as shown).

[0064] In order to evaluate the accuracy of the measurement method of the present invention, a universal tensile measuring machine was used to determine the modulus of the gel material after deconstruction and compared with the measurement results of the present invention (shown in Tables 2 and 3 below). It can be seen from Tables 2 and 3 that the gel equivalent modulus results measured by the two methods are similar, proving the accuracy of the measurement method of the present invention in measuring the mechanical properties of spherical hydrogels with changing mechanical properties.

[0065]

[0066] Table 2 Modulus data of KSK-4 hydrogel after deconstruction in different pH solutions for 10 minutes measured by two methods

[0067]

[0068] Table 3 Modulus of KSK-4 hydrogel after deconstruction in pH=1.2 solution for different time measured by two methods

[0069] Unless otherwise stated, any technical solution disclosed in the present invention disclosed above, if it discloses a numerical range, then the disclosed numerical range is a preferred numerical range, and any technician in the field should understand that the preferred numerical range is only a numerical value with a more obvious technical effect or representative value among many implementable numerical values. Since there are too many numerical values ​​to be exhaustive, the present invention discloses some numerical values ​​to illustrate the technical solution of the present invention, and the numerical values ​​listed above should not constitute a limitation on the scope of protection of the present invention.

[0070] If the words "first", "second" and so on are used in this article to limit components, those skilled in the art should know that the use of "first" and "second" is only for the convenience of description to distinguish the components. Unless otherwise stated, the above words have no special meaning.

[0071] At the same time, if the above-mentioned invention discloses or involves components or structural parts that are fixedly connected to each other, then, unless otherwise stated, the fixed connection can be understood as: a detachable fixed connection (for example, connected by bolts or screws), and can also be understood as: a non-detachable fixed connection (for example, riveting, welding). Of course, the mutual fixed connection can also be replaced by an integrated structure (for example, manufactured by one-piece molding using a casting process) (except when it is obviously impossible to use an integrated molding process).

[0072] In addition, unless otherwise stated, the terms used to indicate positional relationships or shapes in any of the technical solutions disclosed in the present invention include states or shapes that are approximate, similar, or close to them.

[0073] Any component provided by the present invention may be assembled from a plurality of separate components, or may be a separate component manufactured by an integral forming process.

[0074] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, ordinary technicians in the field should understand that the specific implementation methods of the present invention can still be modified or some technical features can be replaced by equivalents without departing from the spirit of the technical solution of the present invention, which should be included in the scope of the technical solution for protection of the present invention.

Claims

1. A working method of a device for real-time measurement of mechanical properties of spherical soft materials, characterized in that: The device for real-time measurement of the mechanical properties of spherical soft materials is used, the device comprises a fluid driver, a first conduit, an observation device and a collection cup arranged in sequence, a channel with a special structure is arranged in the observation device, the fluid driver is connected to the channel via the first conduit, and the channel is connected to the collection cup via the second conduit, so as to drive the spherical soft material into the channel through the fluid power of the fluid driver, so as to realize the real-time measurement of the mechanical properties of the spherical soft material; The channel with a special structure includes one or more of a "T"-shaped channel, a sheath flow channel, a cross-shaped channel, a contraction-shaped channel, and a narrow slit-shaped channel, and the wall of the channel can exert a force on the spherical soft material under the action of fluid loading; The working method comprises the following steps: the fluid force of the fluid driver drives the spherical soft material into a channel with a special structure, and the deformation information and flow field information of the spherical soft material in the channel are obtained through numerical simulation analysis to determine the mechanical properties of the spherical soft material; The deformation information includes one or more of the position and deformation of the spherical soft material when the spherical soft material reaches force equilibrium in the channel, the speed and deformation of the spherical soft material moving in the channel; The flow field information includes channel size, flow field velocity and flow field pressure distribution.

2. The working method of the device for real-time measurement of mechanical properties of spherical soft materials according to claim 1 is characterized in that: The fluid driver is a syringe pump, a hydraulic pump, a pneumatic pump and a peristaltic pump.

3. The working method of the device for real-time measurement of mechanical properties of spherical soft materials according to claim 2 is characterized in that: The fluid driver is a syringe pump, a working end of the syringe pump is provided with a syringe, and a NaCl solution is provided inside the syringe.

4. The working method of the device for real-time measurement of mechanical properties of spherical soft materials according to claim 1 is characterized in that: The first conduit groove and the second conduit groove are respectively arranged on both sides of the channel. The channel is a contraction type channel. The width of the left side of the contraction type channel remains unchanged, and the width of the right side of the contraction type channel decreases successively.

5. The working method of the device for real-time measurement of mechanical properties of spherical soft materials according to claim 1 is characterized in that: The deformation information is the force equilibrium position of the spherical soft material in the channel and the deformation amount at the force equilibrium position.

6. The working method of the device for real-time measurement of mechanical properties of spherical soft materials according to claim 1 is characterized in that: There are two ways of numerical simulation analysis: (1) Determine the mechanical properties of spherical soft materials based on the deformation information and flow field information of spherical soft materials through fluid-solid coupling simulation; (2) First calculate the fluid force on the spherical hydrogel through fluid mechanics simulation, and then calculate the mechanical properties of the spherical soft material through solid mechanics simulation.

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