A circulating centrifugal separation impurity system and a chromatography system thereof

Abstract

The application discloses a circulating centrifugal separation impurity system and a chromatography system thereof. The separation impurity system comprises a buffer tank, a conical bottom outlet of a first cyclone separator and a cylindrical top outlet of a second cyclone separator are connected to the top of the buffer tank, a middle inlet of the first cyclone separator, a middle inlet of the second cyclone separator and a liquid discharge pipeline are connected to the bottom of the buffer tank, a cylindrical top outlet of the first cyclone separator is connected to a first waste discharge pipeline, and a conical bottom outlet of the second cyclone separator is connected to a second waste discharge pipeline. The circulating centrifugal separation impurity system and the chromatography system thereof do not need to flush the screen, can realize rapid separation and improve the efficiency of chromatography.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of chromatography system, in particular to a circulating centrifugal separation impurity system and a chromatography system thereof. BACKGROUND

[0002] The patent with the patent number US20060130444A1 in the prior art introduces a separation device with multiple hydrocyclones in series, and the overflow pipe of each separation stage is connected with the inlet of the next separation stage. The patent with the publication number CN208912333U relates to a multi-stage multi-section hydrocyclone separation device, which has the characteristics of good separation effect and large processing capacity, but the disadvantage is that the separation material stays in the device for a long time and cannot be separated quickly.

[0003] Meanwhile, in the chromatography process, the raw liquid directly flows from the lower mobile phase and out from the upper mobile phase, or the lower mobile phase out and the upper mobile phase in, because there are a large number of impurities in the raw liquid, which will cause the upper and lower screens to be blocked, so it is necessary to flush the screen, and the flushing frequency is increased, which will affect the progress of chromatography separation, thereby affecting the efficiency. SUMMARY

[0004] To solve the above problems, the present application provides a circulating centrifugal separation impurity system and a chromatography system thereof.

[0005] According to one aspect of the present application, a circulating centrifugal separation impurity system is provided, which comprises a buffer tank, the top of the buffer tank is connected with the conical bottom outlet of a first hydrocyclone and the cylindrical top outlet of a second hydrocyclone, respectively, the bottom of the buffer tank is connected with the middle inlet of the first hydrocyclone, the middle inlet of the second hydrocyclone and a liquid discharge pipeline, respectively, wherein the cylindrical top outlet of the first hydrocyclone is connected with a first waste discharge pipeline, and the conical bottom outlet of the second hydrocyclone is connected with a second waste discharge pipeline.

[0006] In some embodiments, the top of the buffer tank is provided with a first inlet, a second inlet and a third inlet, and the bottom is provided with a bottom outlet; wherein the second inlet is connected with the conical bottom outlet of the first hydrocyclone, the third inlet is connected with the cylindrical top outlet of the second hydrocyclone, and the bottom outlet is connected with the middle inlet of the first hydrocyclone, the middle inlet of the second hydrocyclone and the liquid discharge pipeline, respectively. The advantage is that the specific structure and connection mode of the buffer tank are described.

[0007] In some embodiments, a pressure control valve and a pressure sensor are provided on the pipe connecting the second inlet to the conical bottom outlet of the first cyclone separator, and a pressure control valve and a pressure sensor are provided on the pipe connecting the third inlet to the cylindrical top outlet of the second cyclone separator. This is beneficial in that it describes some settings related to the top of the buffer tank and the cyclone separators.

[0008] In some embodiments, a first valve, a pump, a pressure sensor and a flow sensor are provided on the pipe connecting the bottom outlet to the middle inlet of the first cyclone separator, and a second valve, a pump, a pressure sensor and a flow sensor are provided on the pipe connecting the bottom outlet to the middle inlet of the second cyclone separator. This is beneficial in that it describes some settings related to the bottom of the buffer tank and the cyclone separators.

[0009] In some embodiments, a pressure sensor and a flow sensor are provided on the first waste pipe and the second waste pipe, and a third valve is provided on the drain pipe. This is beneficial in that it describes some settings related to the waste pipes.

[0010] According to one aspect of the present application, a chromatography system using the above-mentioned circulating centrifugal separation impurity system is provided, which comprises a chromatography column, a liquid inlet main pipe and a liquid outlet main pipe, the inside of the chromatography column has an upper sieve and a lower sieve, the bottom of the chromatography column has a bottom backflow port, a bottom mobile phase port, and the top of the chromatography column has a plug mobile phase port, wherein the bottom backflow port is connected to the top of the buffer tank through a first pipe, the bottom mobile phase port is connected to the outlet end of the liquid inlet main pipe, and the plug mobile phase port is connected to the inlet of the liquid outlet main pipe.

[0011] In some embodiments, the liquid inlet main pipe and the liquid outlet main pipe are further connected through a seventh valve, and the liquid inlet main pipe is further connected to the drain pipe. This is beneficial in that by connecting the liquid inlet main pipe and the liquid outlet main pipe, the liquid can be drained directly without passing through the chromatography system.

[0012] In some embodiments, the inlet end of the liquid inlet main pipe is connected to the outlet of the buffer solution, the outlet of the liquid to be separated, the outlet of the eluent and the outlet of the cleaning liquid, respectively, and the liquid inlet main pipe is provided with an indicator pump, a transmitter, a pressure transmitter, a conductivity transmitter and a fifth valve. This is beneficial in that it describes some connections and settings related to the liquid inlet main pipe.

[0013] In some embodiments, the outlet end of the main drainage pipe is connected to both the product tank and the waste discharge pipe, and the main drainage pipe is equipped with a sixth valve, a pH sensor, a conductivity transmitter, a flow indicator transmitter, and an ultraviolet sensor. The advantage is that some connections and arrangements related to the main drainage pipe are described.

[0014] In some embodiments, a fourth valve and a pressure transmitter are provided on the first pipeline, and the first pipeline is connected to the main discharge pipeline via an eighth valve. The advantage is that it further describes some specific settings of the chromatography system. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of a circulating centrifugal impurity separation system according to one embodiment of the present invention;

[0016] Figure 2 For an application Figure 1 The diagram shows the structure of the chromatography system for the cyclic centrifugal impurity separation system.

[0017] Figure 3 for Figure 1 A partial structural diagram of the chromatography system is shown.

[0018] In the diagram: Buffer tank 1, First inlet 2, Second inlet 3, Third inlet 4, Bottom outlet 5, First hydrocyclone 6, First valve 7, First waste discharge pipe 8, Second hydrocyclone 9, Second valve 10, Second waste discharge pipe 11, Drain pipe 12, Third valve 13, Chromatography column 20, Bottom reflux port 21, Bottom flow phase port 22, Plunger flow phase port 23, Fifth valve 24, Sixth valve 25, Main inlet pipe 26, Main outlet pipe 27, Seventh valve 28, Product tank 29, Waste discharge pipe 30, Eighth valve 31, Fourth valve 32, First pipe 33. Detailed Implementation

[0019] The present invention will now be described in further detail with reference to the accompanying drawings.

[0020] Figure 1 The structure of a circulating centrifugal impurity separation system according to one embodiment of the present invention is illustrated schematically. Figure 1 As shown, the impurity separation system mainly includes a buffer tank 1, a first cyclone separator 6, a second cyclone separator 9, a first waste discharge pipe 8, and a second waste discharge pipe 11. The buffer tank 1 has a first inlet 2, a second inlet 3, and a third inlet 4 at its top, and a bottom outlet 5 at its bottom. Both the first cyclone separator 6 and the second cyclone separator 9 have a cylindrical top, a middle inlet, and a conical bottom outlet. These structures are interconnected via pipes installed in a specific sequence to form the impurity separation system.

[0021] The second inlet 3 is connected to the conical bottom outlet of the first cyclone separator 6, and a pressure control valve and a pressure sensor (measuring pressure Pu) are arranged on the connecting pipeline. The bottom outlet 5 is connected to the middle inlet of the first cyclone separator 6, and a first valve 7, a pump, a pressure sensor (measuring pressure Pf) and a flow sensor (measuring flow Ff) are arranged on the connecting pipeline. The cylindrical top outlet of the first cyclone separator 6 is connected to the first waste discharge pipeline 8, and a pressure sensor (measuring pressure Po) and a flow sensor (measuring flow Fo) are arranged on the first waste discharge pipeline 8. The pressure difference in the pipeline is DP = Pu - Po, and the overflow ratio is Fo / Ff.

[0022] The third inlet 4 is connected to the cylindrical top outlet of the second cyclone separator 9, and a pressure control valve and a pressure sensor (measuring pressure Po) are arranged on the connecting pipeline. The bottom outlet 5 is connected to the middle inlet of the second cyclone separator 9, and a second valve 10, a pump, a pressure sensor (measuring pressure Pf) and a flow sensor (measuring flow Ff) are arranged on the connecting pipeline. The conical bottom outlet of the second cyclone separator 9 is connected to the second waste discharge pipeline 11, and a pressure sensor (measuring pressure Pu) and a flow sensor (measuring flow Fu) are arranged on the second waste discharge pipeline 11. The pressure difference in the pipeline is DP = Pu - Po, and the overflow ratio is (Ff - Fu) / Ff.

[0023] In addition, the bottom outlet 5 of the buffer tank 1 is also connected to a liquid discharge pipeline 12, and a third valve 13 is arranged on the liquid discharge pipeline 12.

[0024] When the circulating centrifugal separation impurity system is used, the pressure control valve, the first valve 7 and the second valve 10 are opened, and the third valve 13 is closed. The liquid to be separated enters the buffer tank 1 through the first inlet 2, and then the liquid enters the first cyclone separator 6 and the second cyclone separator 9 from the bottom outlet 5 of the buffer tank 1 for separation.

[0025] In the first cyclone separator 6, the oily substance with smaller density in the liquid is discharged through the cylindrical top outlet, and the clear liquid and residue with larger density are discharged through the conical bottom and then re-enter the buffer tank 1. In the second cyclone separator 9, the oil and clear liquid with smaller density in the liquid are discharged through the cylindrical top outlet and then re-enter the buffer tank 1, and the clear liquid and residue with larger density are discharged through the conical bottom. In this way, the liquid is circulated and separated in the buffer tank 1 until only the required clear liquid is left in the tank, and the rest of the oil and residue are discharged from the tank. After the impurity separation work reaches the requirement, the third valve 13 is opened for discharge and delivery.

[0026] In addition, the clear liquid and residue in the two cyclone separators are discharged through the first waste discharge pipeline 8 and the second waste discharge pipeline 11, respectively.

[0027] Figure 2 A structure of a chromatography system applying the circulating centrifugal impurity separation system in Figure 1 is shown, Figure 3 A partial structure of the chromatography system in Figure 2 is shown. As shown in Figures 2-3 , the system mainly includes the continuous centrifugal impurity separation system in Figure 1 , a chromatography column 20, a liquid inlet main pipeline 26, and a liquid discharge main pipeline 27. The chromatography column 20 has an upper sieve and a lower sieve inside, a bottom reflux port 21 and a bottom mobile phase port 22 at the bottom, and a plug mobile phase port 23 at the top.

[0028] The bottom reflux port 21 is connected to the first inlet 2 at the top of the buffer tank 1 through a first pipeline 33, and a fourth valve 32 and a pressure transmitter are arranged on the first pipeline 33.

[0029] The bottom mobile phase port 22 is connected to the outlet end of the liquid inlet main pipeline 26, and the inlet end of the liquid inlet main pipeline 26 is connected to a plurality of liquid pipeline outlets, including a buffer liquid outlet, a liquid to be separated outlet, an eluent outlet, and a cleaning liquid outlet. A pump, a transmitter, a pressure transmitter, a conductivity transmitter, and a fifth valve 24 are arranged on the liquid inlet main pipeline 26. In addition, the liquid inlet main pipeline 26 is also connected to the liquid discharge pipeline 12.

[0030] The plug mobile phase port 23 is connected to the inlet of the liquid discharge main pipeline 27, and the outlet end of the liquid discharge main pipeline 27 is connected to a product tank 29 and a waste discharge pipeline 30. A sixth valve 25, a pH sensor, a conductivity transmitter, a flow indicator transmitter, and an ultraviolet sensor are arranged on the liquid discharge main pipeline 27.

[0031] In addition, the liquid inlet main pipe 26 and the liquid outlet main pipe 27 are connected through the seventh valve 28, and the first pipe 33 is connected with the liquid outlet main pipe 27 through the eighth valve 31.

[0032] When the chromatography system is used, the liquid to be separated is introduced into the chromatography column 20 through the liquid inlet main pipe 26, and a part of the liquid enters the main body through the lower sieve and then flows out from the plunger mobile phase port 21 after passing through the upper sieve, and then is introduced into the waste pipe 19 through the liquid outlet main pipe 27.

[0033] Another part of the liquid does not enter the chromatography column 20, but enters the buffer tank 1 through the pipe after passing through the backflow port 21 at the bottom of the chromatography column 20 for separation, and the clear liquid with a larger density after separation is discharged through the discharge pipe 12 and then introduced into the liquid inlet main pipe 26, and at this time, the fourth valve 32 is closed, so that the clear liquid can be purified through the chromatography column 20.

[0034] When the liquid does not need to pass through the chromatography column 20, only the seventh valve 28 needs to be opened, and at this time, the liquid will pass through the liquid inlet main pipe 26, the seventh valve 28 and the liquid outlet main pipe 27, and then be introduced into the product tank 29 or the waste pipe 30 according to the setting.

[0035] When elution is needed, the eighth valve 31, the fifth valve 24 and the sixth valve 25 are opened, the eluent is introduced into the chromatography column 20 through the liquid inlet main pipe 26, and then is introduced into the liquid outlet main pipe 27 through the backflow port 21 at the bottom of the chromatography column 20 and the plunger mobile phase port 23 of the chromatography column 20 respectively, and finally is introduced into the product tank 29.

[0036] The above only describes some embodiments of the present application. Those skilled in the art can make several modifications and improvements without departing from the concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A chromatography system employing a circulating centrifugal separation of impurities system, characterized by: The circulating centrifugal separation impurity system comprises a buffer tank (1), a conical bottom outlet of a first cyclone separator (6) and a cylindrical top outlet of a second cyclone separator (9) are connected to the top of the buffer tank (1) respectively, a middle inlet of the first cyclone separator (6), a middle inlet of the second cyclone separator (9) and a liquid discharge pipeline (12) are connected to the bottom of the buffer tank (1) respectively, wherein a first waste discharge pipeline (8) is connected to the cylindrical top outlet of the first cyclone separator (6), a second waste discharge pipeline (11) is connected to the conical bottom outlet of the second cyclone separator (9), and a pressure sensor and a flow sensor are arranged on the first waste discharge pipeline (8) and the second waste discharge pipeline (11) respectively, and a third valve (13) is arranged on the liquid discharge pipeline (12). The top of the buffer tank (1) is provided with a first inlet (2), a second inlet (3) and a third inlet (4), and the bottom is provided with a bottom outlet (5); wherein the second inlet (3) is connected to the conical bottom outlet of the first cyclone separator (6), the third inlet (4) is connected to the cylindrical top outlet of the second cyclone separator (9), and the bottom outlet (5) is connected to the middle inlet of the first cyclone separator (6), the middle inlet of the second cyclone separator (9) and the liquid discharge pipeline (12) respectively. The chromatography system comprises a chromatography column (20), a liquid inlet main pipeline (26) and a liquid discharge main pipeline (27), the inside of the chromatography column (20) has an upper screen and a lower screen, the bottom of the chromatography column (20) has a bottom backflow port (21) and a bottom mobile phase port (22), and the top of the chromatography column (20) has a plunger mobile phase port (23), wherein the bottom backflow port (21) is connected to the top of the buffer tank (1) through a first pipeline (33), the bottom mobile phase port (22) is connected to the outlet end of the liquid inlet main pipeline (26), and the plunger mobile phase port (23) is connected to the inlet of the liquid discharge main pipeline (27).

2. The chromatography system of claim 1, wherein the system is applied to a system for cyclically centrifuging impurities, characterized in that: A pressure control valve and a pressure sensor are arranged on the pipeline connecting the second inlet (3) and the conical bottom outlet of the first cyclone separator (6), and a pressure control valve and a pressure sensor are arranged on the pipeline connecting the third inlet (4) and the cylindrical top outlet of the second cyclone separator (9).

3. The chromatography system of claim 1, wherein the system is applied to a system for cyclically centrifuging impurities, characterized in that: A first valve (7), a pump, a pressure sensor and a flow sensor are arranged on the pipeline connecting the bottom outlet (5) and the middle inlet of the first cyclone separator (6), and a second valve (10), a pump, a pressure sensor and a flow sensor are arranged on the pipeline connecting the bottom outlet (5) and the middle inlet of the second cyclone separator (9).

4. The chromatography system of claim 1, wherein the system is applied to a system for centrifugally separating impurities in a cycle. The liquid inlet main pipeline (26) and the liquid discharge main pipeline (27) are further connected through a seventh valve (28), and the liquid inlet main pipeline (26) is further connected to the liquid discharge pipeline (12).

5. The chromatography system of claim 1, wherein the system is applied to a system for cyclically centrifuging impurities, characterized in that: The import end of the liquid inlet main pipeline (26) is connected with the buffer liquid outlet, the liquid to be separated outlet, the eluent outlet and the cleaning liquid outlet respectively, and the liquid inlet main pipeline (26) is provided with an indicating pump, a transmitter, a pressure transmitter, a conductivity transmitter and a fifth valve (24).

6. The chromatography system of claim 1, wherein the system is applied to a system for centrifugally separating impurities in a cycle. The outlet end of the liquid outlet main pipeline (27) is connected with a product tank (29) and a waste pipeline (30) respectively, and the liquid outlet main pipeline (27) is provided with a sixth valve (25), a pH sensor, a conductivity transmitter, a flow indicating transmitter and an ultraviolet sensor.

7. The chromatography system of claim 1, wherein the system is applied to a system for centrifugally separating impurities in a cycle. The first pipeline (33) is provided with a fourth valve (32) and a pressure transmitter, and the first pipeline (33) is communicated with the liquid outlet main pipeline (27) through an eighth valve (31).

Images