A switching centrifugal separation impurity system and chromatography system thereof

Abstract

The application discloses a switching centrifugal separation impurity system and a chromatography system thereof. The switching centrifugal separation impurity system comprises a buffer tank, a cyclone separator and a four-valve array group, the bottom of the buffer tank is connected with the middle outlet of the cyclone separator, wherein the cylindrical top of the cyclone separator, the top of the buffer tank and the conical bottom of the cyclone separator are respectively connected between different valves of the four-valve array group. The switching centrifugal separation impurity system and the chromatography system thereof can realize rapid separation without washing the screen, thereby improving the efficiency of the chromatography.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of chromatography systems, in particular to a switching centrifugal separation impurity system and a chromatography system thereof. BACKGROUND

[0002] The patent with the patent number of US20060130444A1 in the prior art introduces a separation device with multiple hydrocyclones in series, and the overflow pipe of each separation stage is connected with the inlet of the next separation stage. The patent with the publication number of CN208912333U relates to a multi-stage multi-section hydrocyclone separation device, which has the characteristics of good separation effect and large processing capacity, but the disadvantage is that the separation material stays in the device for a long time and cannot achieve rapid separation.

[0003] At the same time, in the chromatography process, the stock solution directly flows from the lower mobile phase and out from the upper mobile phase, or the lower mobile phase out and the upper mobile phase in, because there are a large number of impurities in the stock solution, which will cause the upper and lower screens to be blocked, so it is necessary to flush the screen, and the frequency of flushing will increase, which will affect the progress of chromatography separation, thereby affecting the efficiency. SUMMARY

[0004] To solve the above problems, the present application provides a switching centrifugal separation impurity system and a chromatography system thereof.

[0005] According to one aspect of the present application, a switching centrifugal separation impurity system is provided, comprising a buffer tank, a cyclone separator and a four-valve array, the bottom of the buffer tank is connected with the middle outlet of the cyclone separator, wherein the cylindrical top of the cyclone separator, the top of the buffer tank and the conical bottom of the cyclone separator are respectively connected between different valves of the four-valve array.

[0006] In some embodiments, the top of the buffer tank has a first inlet and a second inlet, and the bottom has a bottom outlet; wherein the second inlet is connected with the four-valve array, and the bottom outlet is connected with the middle outlet of the cyclone separator through a circulating pipeline. Its advantage lies in that the specific structure and connection mode of the buffer tank are described.

[0007] In some embodiments, the four-valve array has valves a, b, c and d in sequence, wherein the cylindrical top of the cyclone separator is connected between valve a and valve b, the top of the buffer tank is connected between valve b and valve c, the conical bottom of the cyclone separator is connected between valve c and valve d, and a waste pipe is connected between valve d and valve a. This is beneficial in that the specific structure and connection of the four-valve array are described.

[0008] In some embodiments, the cylindrical top of the cyclone separator is connected to the four-valve array through an upper outlet pipe, and the conical bottom of the cyclone separator is connected to the four-valve array through a lower outlet pipe. This is beneficial in that the relevant structure of the system is further described.

[0009] In some embodiments, the circulation pipe is provided with a first valve, a pump, a flow sensor and a pressure sensor, the upper outlet pipe is provided with a flow sensor, a pressure control valve and a pressure sensor, and the lower outlet pipe is provided with a pressure control valve and a pressure sensor. This is beneficial in that some settings on the pipes are described.

[0010] In some embodiments, the bottom outlet of the buffer tank is connected to a liquid discharge pipe, and the liquid discharge pipe is provided with a second valve. This is beneficial in that the separated liquid in the system can be discharged through the liquid discharge pipe.

[0011] According to one aspect of the present application, a chromatography system using the above-mentioned switching centrifugal separation impurity system is provided, which comprises a chromatography column, a liquid inlet main pipe and a liquid outlet main pipe, the inside of the chromatography column has an upper screen and a lower screen, the bottom of the chromatography column has a bottom reflux port, a bottom mobile phase port, and the top of the chromatography column has a plunger mobile phase port, wherein the bottom reflux port is connected to the top of the buffer tank through a first pipe, the first pipe is provided with a third valve and a pressure transmitter, the bottom mobile phase port is connected to the outlet end of the liquid inlet main pipe, the plunger mobile phase port is connected to the inlet end of the liquid outlet main pipe, and the liquid inlet main pipe is connected to the bottom outlet of the buffer tank.

[0012] In some embodiments, the first pipe is connected to the liquid outlet main pipe through a sixth valve, and the liquid inlet main pipe and the liquid outlet main pipe are connected through a seventh valve. This is beneficial in that the liquid can be directly discharged without passing through the chromatography system by connecting the liquid inlet main pipe and the liquid outlet main pipe.

[0013] In some embodiments, the inlet end of the inlet main pipe is connected to the buffer outlet, the liquid to be separated outlet, the eluent outlet and the cleaning liquid outlet respectively, and the inlet main pipe is provided with an indicator pump, a transmitter, a pressure transmitter, a conductivity transmitter and a fourth valve. Its benefits are that some connections and settings related to the inlet main pipe are described.

[0014] In some embodiments, the outlet end of the outlet main pipe is connected to the product tank and the waste pipe respectively, and the outlet main pipe is provided with a fifth valve, a pH sensor, a conductivity transmitter, a flow indicator transmitter and an ultraviolet sensor. Its benefits are that some connections and settings related to the outlet main pipe are described. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 A structure schematic diagram of a switching centrifugal separation impurity system according to an embodiment of the present application;

[0016] Figure 2 A structure schematic diagram of a chromatography system using the switching centrifugal separation impurity system shown in Figure 1

[0017] Figure 3 A partial structure schematic diagram of the chromatography system shown in Figure 1

[0018] In the figure: buffer tank 1, second inlet 2, first inlet 3, bottom outlet 4, circulation pipe 5, first valve 6, second valve 7, cyclone separator 8, upper outlet pipe 9, lower outlet pipe 10, four-valve array 11, liquid discharge pipe 12, chromatography column 20, bottom backflow port 21, bottom mobile phase port 22, plunger mobile phase port 23, fourth valve 24, fifth valve 25, inlet main pipe 26, outlet main pipe 27, seventh valve 28, product tank 29, waste pipe 30, sixth valve 31, third valve 32, first pipeline 33. DETAILED DESCRIPTION

[0019] The present application will be further described in detail below with reference to the accompanying drawings.

[0020] Figure 1 A structure of a switching centrifugal separation impurity system according to an embodiment of the present application is schematically shown. As shown in Figure 1 ​​As shown, the impurity separation system mainly comprises a buffer tank 1, a cyclone separator 8 and a four-valve array 11. The buffer tank 1 has a first inlet 3 and a second inlet 2 at the top and a bottom outlet 4 at the bottom. The cyclone separator 8 has a cylindrical top, a middle inlet and a conical bottom outlet. The four-valve array 11 has four valves connected in sequence, and the valves are arranged in the order of valve a, valve b, valve c and valve d. The above structures are connected in a specific sequence by pipelines to form the impurity separation system.

[0021] The bottom outlet 4 of the buffer tank 1 is connected to the middle outlet of the cyclone separator 8 through a circulation pipeline 5. The circulation pipeline 5 is provided with a first valve 6, a pump, a flow sensor (measuring flow Ff) and a pressure sensor (measuring pressure Pf).

[0022] The cylindrical top of the cyclone separator 8 is connected between valve a and valve b of the four-valve array 11 through an upper outlet pipeline 9. The upper outlet pipeline 9 is provided with a flow sensor (measuring flow Fo), a pressure control valve and a pressure sensor (measuring pressure Po). The conical bottom of the cyclone separator 8 is connected between valve c and valve d of the four-valve array 11 through a lower outlet pipeline 10. The lower outlet pipeline 10 is provided with a pressure control valve and a pressure sensor (measuring pressure Pu). Therefore, the pressure difference at this point is DP = Pu - Po, and the overflow ratio is Fo / Ff.

[0023] The second inlet 2 of the top of the buffer tank 1 is connected between valve b and valve c of the four-valve array 11. A waste pipeline is connected between valve d and valve a of the four-valve array 11.

[0024] In addition, the valves of the four-valve array 11 can also be connected in other reasonable ways, such as connecting the upper outlet pipeline 9 between valve b and valve c, connecting the lower outlet pipeline 10 between valve b and valve d, connecting the waste pipeline between valve a and valve b, and connecting the second inlet 2 between valve c and valve d.

[0025] The bottom outlet 4 of the buffer tank 1 is connected to a liquid discharge pipeline 12, and the liquid discharge pipeline 12 is provided with a second valve 7.

[0026] When using this switching centrifugal impurity separation system, the liquid to be separated is introduced into the buffer tank 1 through the first inlet 3. At this time, the first valve 6, valve b and valve c are opened, and the liquid to be separated enters the hydrocyclone 8 through the circulation pipe 5. After being separated by the hydrocyclone 8, the denser slag material enters the waste discharge pipe through the lower outlet pipe 10, and the other less dense materials enter the buffer tank 1 through the upper outlet pipe 9. This hydrocyclone separation is repeated many times until the material to be separated does not contain any dense slag material.

[0027] Then, close valves b and c, and open valves a and d. The substance to be separated enters the hydrocyclone separator 8 through the circulation pipe 5. After being separated by the hydrocyclone separator 8, the denser clear liquid enters the buffer tank through the lower outlet pipe 10, and the less dense oily substance enters the waste discharge pipe through the upper outlet pipe 9. This hydrocyclone separation is repeated many times to concentrate the substance in the tank.

[0028] Figure 2 It shows an application Figure 1 The structure of the chromatography system in the switching centrifugal impurity separation system, Figure 3 Showing Figure 2 Partial structure of the chromatography system. For example... Figures 2-3 As shown, the system mainly includes Figure 1 The system includes a centrifugal impurity separation system, a chromatography column 20, a main inlet pipe 26, and a main outlet pipe 27. The chromatography column 20 has an upper screen and a lower screen inside, a bottom reflux port 21 and a bottom flow phase port 22 at the bottom, and a plunger flow phase port 23 at the top.

[0029] The bottom reflux port 21 is connected to the first inlet 3 at the top of the buffer tank 1 through the first pipeline 33, and a third valve 32 and a pressure transmitter are installed on the pipeline 33.

[0030] The bottom flow port 22 is connected to the outlet end of the main inlet pipe 26, while the inlet end of the main inlet pipe 26 is connected to multiple liquid outlet pipes, including a buffer solution outlet, a liquid to be separated outlet, an eluent outlet, and a washing solution outlet. The main inlet pipe 26 is equipped with an indicating pump, a transmitter, a pressure transmitter, a conductivity transmitter, and a fourth valve 24. Furthermore, the main inlet pipe 26 is also connected to the drain pipe 12, specifically to the bottom outlet 4 of the buffer tank 1.

[0031] The flow port 23 is connected to the inlet of the main discharge pipe 27, while the outlet of the main discharge pipe 27 is connected to the product tank 29 and the waste discharge pipe 30. The main discharge pipe 27 is equipped with a fifth valve 25, a pH sensor, a conductivity transmitter, a flow indicator transmitter, and an ultraviolet sensor.

[0032] In addition, the first pipeline 33 is also connected with the liquid outlet main pipeline 27 through the sixth valve 31, and the liquid inlet main pipeline 26 is also connected with the liquid outlet main pipeline 27 through the seventh valve 28.

[0033] When the chromatography system is used, the liquid to be separated enters into the chromatography column 20 through the liquid inlet main pipeline 26, wherein a part of the liquid enters into the main body through the lower sieve net, then flows out from the plunger mobile phase port 21 after passing through the upper sieve net, and then enters into the waste pipeline 19 through the liquid outlet main pipeline 27.

[0034] Another part of the liquid does not enter into the chromatography column 20, but enters into the buffer tank 1 through the pipeline after passing through the backflow port 21 at the bottom of the chromatography column 20, and when the tank reaches a certain amount, the third valve 32 is closed to separate and concentrate the liquid, and the liquid after separation and concentration enters into the liquid inlet main pipeline 26 through the liquid discharge pipeline 12, and then enters into the chromatography column 20 for purification, and finally is discharged through the liquid outlet main pipeline 27.

[0035] When the liquid does not need to pass through the chromatography column 20, only the seventh valve 28 needs to be opened, at this time, the liquid will enter into the product tank 29 or the waste pipeline 30 according to the setting after passing through the liquid inlet main pipeline 26, the seventh valve 28 and the liquid discharge pipeline 27.

[0036] When elution is needed, the sixth valve 31, the fourth valve 24 and the fifth valve 25 need to be opened, the elution liquid enters into the chromatography column 20 through the liquid inlet main pipeline 26, then enters into the liquid outlet main pipeline 27 through the backflow port 21 at the bottom of the chromatography column 20 and the plunger mobile phase port 23 of the chromatography column 20 respectively, and finally enters into the product tank 29.

[0037] The above only describes some embodiments of the present application. Those skilled in the art can make several modifications and improvements without departing from the concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A chromatography system applying a switched centrifugal separation impurity system, characterized by: The switching centrifugal separation impurity system comprises a buffer tank (1), a cyclone separator (8) and a four-valve array (11), the bottom of the buffer tank (1) is connected with the middle outlet of the cyclone separator (8), wherein the cylindrical top of the cyclone separator (8), the top of the buffer tank (1) and the conical bottom of the cyclone separator (8) are connected between different valves of the four-valve array (11) respectively. The chromatography system comprises a chromatography column (20), a liquid inlet main pipeline (26) and a liquid outlet main pipeline (27), the inside of the chromatography column (20) has upper and lower screens, the bottom of the chromatography column (20) has a bottom backflow port (21) and a bottom mobile phase port (22), and the top of the chromatography column (20) has a plunger mobile phase port (23), wherein the bottom backflow port (21) is connected with the top of the buffer tank (1) through a first pipeline (33), the first pipeline (33) is provided with a third valve (32) and a pressure transmitter, the bottom mobile phase port (22) is connected with the outlet end of the liquid inlet main pipeline (26), the plunger mobile phase port (23) is connected with the inlet end of the liquid outlet main pipeline (27), and the liquid inlet main pipeline (26) is connected with the bottom outlet (4) of the buffer tank (1). The first pipeline (33) is connected with the liquid outlet main pipeline (27) through a sixth valve (31), and the liquid inlet main pipeline (26) and the liquid outlet main pipeline (27) are communicated through a seventh valve (28). The inlet end of the liquid inlet main pipeline (26) is connected with a buffer liquid outlet, a liquid to be separated outlet, an eluent outlet and a cleaning liquid outlet respectively, and the liquid inlet main pipeline (26) is provided with an indicating pump, a transmitter, a pressure transmitter, a conductivity transmitter and a fourth valve (24).

2. A chromatography system applying a switching centrifugal separation impurities system according to claim 1, characterized in that: The top of the buffer tank (1) has a first inlet (3) and a second inlet (2), and the bottom of the buffer tank (1) has a bottom outlet (4); wherein the second inlet (2) is connected with the four-valve array (11), and the bottom outlet (4) is connected with the middle outlet of the cyclone separator (8) through a circulation pipeline (5).

3. A chromatography system employing a switching centrifugal separation impurity system according to claim 2, characterized in that: The four-valve array (11) has a valve a, a valve b, a valve c and a valve d which are sequentially adjacent, wherein the cylindrical top of the cyclone separator (8) is connected between the valve a and the valve b, the top of the buffer tank (1) is connected between the valve b and the valve c, the conical bottom of the cyclone separator (8) is connected between the valve c and the valve d, and a waste pipeline is connected between the valve d and the valve a.

4. The chromatography system of claim 2 applied with a switching centrifugal separation impurities system, characterized in that: The cylindrical top of the cyclone separator (8) is connected with the four-valve array (11) through an upper outlet pipeline (9), and the conical bottom of the cyclone separator (8) is connected with the four-valve array (11) through a lower outlet pipeline (10).

5. A chromatography system employing a switching centrifugal separation impurity system according to claim 4, characterized in that: The circulating pipeline (5) is provided with a first valve (6), a pump, a flow sensor and a pressure sensor, the upper outlet pipeline (9) is provided with a flow sensor, a pressure control valve and a pressure sensor, and the lower outlet pipeline (10) is provided with a pressure control valve and a pressure sensor.

6. The chromatography system of claim 2 applied with a switching centrifugal separation impurities system, characterized in that: The bottom outlet (4) of the buffer tank (1) is connected with a liquid discharge pipeline (12), and the liquid discharge pipeline (12) is provided with a second valve (7).

7. The chromatography system of claim 1, wherein the system is applied to a switching centrifugal separation impurity system. The outlet end of the liquid outlet main pipeline (27) is connected with a product tank (29) and a waste discharge pipeline (30) respectively, and the liquid outlet main pipeline (27) is provided with a fifth valve (25), a pH sensor, a conductivity transmitter, a flow indication transmitter and an ultraviolet sensor.

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