Metallic platinum mass tags for mass spectrometric flow cytometry detection

Abstract

The application provides a metal platinum mass tag for mass spectrometry flow detection, which has a ligand of platinum covalently combined with metal platinum, the ligand is combined with a main chain through linker2, the main chain is connected with linker1, the linker1 allows combination with a biomolecule, and the main chain is a water-soluble polymer. The application has the advantages of difficult overflow of platinum ions, no toxicity to cells and the like.

Description

TECHNICAL FIELD

[0001] The present application relates to mass spectrometry flow detection, in particular to a metal platinum mass label for mass spectrometry flow detection. BACKGROUND

[0002] There are about 100 isotopes that can be theoretically applied to mass spectrometry flow testing, but only about 50 are actually applied. In order to improve the multi-channel testing capability of mass spectrometry flow, more available detection parameters need to be supplemented. Secondly, the current metal ligand such as DOTA, DTPA, EDTA only has good chelating effect on hard metal ions (such as rare earth and Bi3+ and the like), but the chelating ability for soft metal (for example, ruthenium, platinum) is poor, causing platinum ion overflow, which affects the biological activity of cells and antibodies. SUMMARY

[0003] In order to solve the above problems in the prior art, the present application provides a metal platinum mass label for mass spectrometry flow detection.

[0004] The purpose of the present application is achieved by the following technical solutions:

[0005] The metal platinum mass label for mass spectrometry flow detection has a ligand of platinum covalently combined with metal platinum, the ligand is combined with a main chain through linker2, the main chain is connected with linker1, the linker1 allows to be combined with a biological molecule, and the main chain is a water-soluble polymer.

[0006] Compared with the prior art, the present application has the beneficial effects that:

[0007] The ligand of the present application is covalently combined with platinum metal, which is more stable than the ligand chelate combined in the prior art in a coordination manner, platinum metal ions are not easy to overflow, and has no toxicity to cells, while not affecting the detection ability of the antibody; secondly, the mass label preparation process has safety, economy, and simple use process. BRIEF DESCRIPTION OF DRAWINGS

[0008] The disclosure of the present application will become more apparent with reference to the accompanying drawings. It is easy for those skilled in the art to understand that these drawings are only used to illustrate the technical solutions of the present application, and are not intended to limit the protection scope of the present application. In the drawings:

[0009] Figure 1 is a structure schematic diagram of the metal platinum mass label for mass spectrometry flow detection according to the embodiment of the present application;

[0010] Figure 2 is a preparation schematic diagram of the ligand according to the embodiment of the present application;

[0011] Figure 3is a schematic diagram of the preparation of another ligand according to an embodiment of the present application. DETAILED DESCRIPTION

[0012] Figures 1-3 The alternative embodiments of the present application described below are presented to teach those skilled in the art how to make and use the present application. Some conventional aspects have been simplified or omitted to teach the underlying principles of the present application. Those skilled in the art should appreciate that the variations and modifications they devise that depart from the embodiments described herein fall within the bounds of the present application. Those skilled in the art should appreciate that the features described below can be combined in various ways to form multiple variations of the present application. As such, the present application is not limited to the alternative embodiments described below, but rather only by the claims and their equivalents.

[0013] Example 1

[0014] Figure 1 is a schematic diagram of a metal platinum mass tag for mass spectrometric flow cytometry according to an embodiment of the present application, as Figure 1 shown, the metal platinum mass tag has:

[0015] a ligand R covalently bound to platinum, the ligand R is bound to a backbone through linker 2, the backbone is connected to linker 1, the linker 1 allows binding to a biomolecule, the backbone is a water-soluble polymer;

[0016] the linker 1 and / or linker 2 is click chemistry of alkyne with azide, thiol with alkene, amine with active ester to form amide bond, hydroxyl with carboxyl to form ester, or biotin-avidin;

[0017] the ligand is DPA, or a compound comprising cisplatin, carboplatin, oxaliplatin, nedaplatin, or lobaplatin structure and modification.

[0018] Example 2

[0019] Application example of a metal platinum mass tag for mass spectrometric flow cytometry according to embodiment 1 of the present application.

[0020] In this application example, as Figure 2 shown, a ligand R covalently bound to platinum is prepared, the ligand R comprises a compound of cisplatin structure X is Cl, L is NH2;

[0021] The ligand RR is modified so that the ligand is bound to a backbone to obtain a metal platinum mass tag, as Figure 1 shown;

[0022] As Figure 1As shown, linker1 and / or linker2 is any one selected from the following: click chemistry of alkyne and azide, thiol and olefin, amide bond formed by amine and active ester, ester formed by hydroxyl and carboxyl, or biotin-avidin.

[0023] Example 3:

[0024] According to the application example of the metal platinum mass tag for mass spectrometry flow detection according to embodiment 1, different from embodiment 2 is that:

[0025] In the manner as shown in Figure 3 The ligand R is prepared.

Claims

1. Metal platinum mass tag for mass spectrometric flow cytometry, said metal platinum mass tag for mass spectrometric flow cytometry having a ligand for platinum covalently bound to metal platinum, which ligand is bound to a backbone via linker2, said backbone is connected to linker1, said linker1 allows binding to a biomolecule, said backbone is a water soluble polymer; The structure of the ligand is X is halogen or a group containing oxygen, sulfur, L is a nitrogen-containing group or a compound of nitrogen-containing structure.

2. The metal platinum mass tag for mass spectrometric flow detection of claim 1, wherein, said ligand is DPA, or a compound comprising cisplatin, carboplatin, oxaliplatin, nedaplatin or lobaplatin structure and modifications.

3. The metal platinum mass tag for mass spectrometric flow detection of claim 1, wherein, two L are connected by a chemical bond and extend a site for binding to the backbone.

4. The metal platinum mass tag for mass spectrometric flow detection of claim 1, wherein, said linker1 and / or linker2 is click chemistry of alkyne with azide, thiol with alkene, amide bond formation of amine with active ester, ester formation of hydroxyl with carboxyl, or biotin-avidin.

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