Cell phase transition-based photosensitive imaging device and information storage method thereof

Abstract

The application relates to the field of photosensitive imaging, and provides a photosensitive imaging device based on cell phase change and an information storage method thereof. The photosensitive imaging device comprises a photosensitive phase change unit, a phase change detection unit and an image restoration unit. The photosensitive phase change unit comprises a photosensitive phase change cell layer, an environmental matrix and a packaging shell, and the photosensitive phase change cell layer is composed of a plurality of photosensitive phase change cells containing recombinant photosensitive phase change proteins. The environmental matrix and the photosensitive phase change cell layer are both packaged in the packaging shell. The phase change detection unit detects cell phase change information generated by the photosensitive phase change unit under light intensity information of a target image. The image restoration unit restores the cell phase change information into the light intensity information, and converts the light intensity information into gray scale information, so as to obtain the target image. According to the photosensitive imaging device, photosensitive imaging is realized through the phase change characteristics of the photosensitive phase change cells under light, and the device is based on natural life materials, can be reused without using a silicon circuit, and is green and environment-friendly.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of photoreception imaging, and in particular to a photoreception imaging device based on cell phase transition and an information storage method thereof. BACKGROUND

[0002] At present, common photoreception imaging devices are mostly chemical photoreception films or photoelectric devices. The chemical photoreception film imaging technology is mature and has good imaging effect; however, the developing and washing procedures are complex, and the chemical photoreception film cannot be repeatedly imaged usually, and the materials used in the manufacturing and washing processes can easily cause environmental pollution. The photoreception imaging of the photoelectric device has the characteristics of rapid response, repeated use, convenient storage and transmission of image information, etc.; however, the photoelectric conversion efficiency of the photoreception imaging device based on silicon electric materials is low, which limits the pixel size to be refined to microns, and the poor biocompatibility and the environmental pollution caused by the production and manufacturing process further limit the sustainable use of the silicon electric materials. SUMMARY

[0003] The present application aims at at least solving one of the problems in the prior art. To this end, the present application provides a photoreception imaging device based on cell phase transition.

[0004] The present application also provides an information storage method of the photoreception imaging device based on cell phase transition.

[0005] The present application also provides an electronic device and a non-transitory computer storage medium.

[0006] The photoreception imaging device based on cell phase transition according to the first aspect of the present application comprises:

[0007] A photoreception phase transition unit, comprising a photoreception phase transition cell layer, an environment matrix and a packaging shell, the photoreception phase transition cell layer comprising a plurality of photoreception phase transition cells containing recombinant photosensitive phase transition proteins, the photoreception phase transition cells being adapted to undergo phase transition under light and to convert light intensity information into cell phase transition information; the environment matrix being adapted to provide a suitable living environment for the photoreception phase transition cells, the environment matrix and the photoreception phase transition cell layer being packaged in the packaging shell;

[0008] A phase transition detection unit for detecting the cell phase transition information generated by the photoreception phase transition unit under the light intensity information of a target image;

[0009] An image restoration unit, which internally stores the corresponding relationship between the cell phase transition information and the light intensity under different exposure times, the image restoration unit being used for restoring the cell phase transition information detected by the phase transition detection unit into light intensity information, and converting the light intensity information into gray scale information to obtain the target image.

[0010] According to one embodiment of the present application, the light-sensitive phase change unit further comprises pixel division wells, which are well-shaped three-dimensional structures, and the pixel division wells form a plurality of mutually independent grid spaces;

[0011] The light-sensitive phase change cells of the light-sensitive phase change cell layer are uniformly distributed in each of the grid spaces, and each of the grid spaces is filled with the environmental matrix, and the light-sensitive phase change cell layer, the environmental matrix, and the pixel division wells are all encapsulated in the encapsulation shell.

[0012] The light-sensitive phase change unit is specifically used for converting the light intensity information of the target image into cell phase change information of the light-sensitive phase change cells in each grid space of the pixel division wells, the phase change detection unit is specifically used for detecting the cell phase change information of the light-sensitive phase change cells in each grid space of the pixel division wells, and the image restoration unit is specifically used for restoring the cell phase change information of the light-sensitive phase change cells in each grid space detected by the phase change detection unit into light intensity information at each grid space.

[0013] According to one embodiment of the present application, the recombinant light-sensitive phase change protein is a protein formed by combining the corresponding base sequences of a light-sensitive protein and an intrinsic disordered protein domain, and the protein is constructed by transfecting the protein into cells through a plasmid or a lentivirus as a carrier and expressing the protein in the cells.

[0014] The phase change detection unit comprises a bright field microscope, and the bright field microscope is used for photographing the light-sensitive phase change cells to obtain a bright field image, and the unit area average gray value of the phase change aggregation part of the light-sensitive phase change cells in the bright field image is the cell phase change information.

[0015] According to one embodiment of the present application, the recombinant light-sensitive phase change protein is a protein formed by combining the corresponding base sequences of a light-sensitive protein, a fluorescent protein, and an intrinsic disordered protein domain, and the protein is constructed by transfecting the protein into cells through a plasmid or a lentivirus as a carrier and expressing the protein in the cells.

[0016] The phase change detection unit comprises a fluorescence microscope, and the fluorescence microscope is used for photographing the light-sensitive phase change cells to obtain a fluorescence image, and the unit area fluorescence intensity of the phase change aggregation part of the light-sensitive phase change cells in the fluorescence image is the cell phase change information.

[0017] According to one embodiment of the present application, the corresponding relationship between the cell phase change information of the light-sensitive phase change cells and the light intensity under different exposure times stored in the image restoration unit is determined through a pre-experiment.

[0018] According to one embodiment of the present application, the light-sensitive protein comprises Arabidopsis Cry2 protein or human ChR2 protein, and the intrinsic disordered protein domain comprises FUS, DDX4, or HNRNPA1.

[0019] According to an embodiment of the present application, the light-sensitive phase-change cell layer further comprises a light-sensitive performance enhancer.

[0020] The light-sensitive performance enhancer comprises a light-sensitive protein, up-conversion nanoparticles or metal nanoparticles independent of the light-sensitive phase-change cell.

[0021] According to an embodiment of the present application, the pixel division well and the packaging shell are both prepared by an additive manufacturing method.

[0022] According to an embodiment of the present application, the length and width of the single grid space of the pixel division well ranges from 10 μm x 10 μm to 200 μm x 200 μm, and the depth ranges from 100 μm to 500 μm.

[0023] In summary, the cell phase-change based light-sensitive imaging device according to the embodiments of the present application is based on natural life materials (i.e. light-sensitive phase-change cells), does not need to use a combination of silicon circuits and can be reused, does not need to use a chemical heavy metal product with high pollution, and is easy to degrade after use, thus being environmentally friendly. Moreover, the light sensitivity and light-sensitive range of the light-sensitive phase-change cell layer can be adjusted, the type of light-driven phase-change light-sensitive protein can be adjusted by adjusting the base fragments of the imported cells, the response range and degree to external light can be adjusted, and the response range and degree to external light can be adjusted by adjusting the light-sensitive performance enhancer.

[0024] In addition, the pixel division well can prevent the migration of cells during use and form relatively independent pixels. The pixel division well can be manufactured by an additive technology such as two-photon photocuring, so as to achieve a resolution close to that of the existing advanced technology, and the energy conversion efficiency is higher than that of a traditional silicon photoelectric circuit and a light-sensitive imaging device using only biological molecules, and has the potential to further improve the resolution.

[0025] According to the information storage method based on the cell phase-change based light-sensitive imaging device according to the first aspect of the second aspect embodiment of the present application, comprising:

[0026] Based on the detection limit and phase-change sensitivity of the light-sensitive phase-change cell, a plurality of different light intensities are obtained, and the plurality of light intensities are encoded according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding. The detection limit and phase-change sensitivity of the light-sensitive phase-change cell are obtained based on a pre-experiment.

[0027] The information to be stored is encoded according to the preset encoding principle to obtain an encoding set corresponding to the information to be stored;

[0028] According to the standard correspondence table, a light intensity set corresponding to the encoding set is obtained.

[0029] Irradiate the photosensitive phase change cells with the light in the set of light intensities to obtain phase change information of the photosensitive phase change cells, and store the information to be stored as the phase change information of the photosensitive phase change cells, wherein each light intensity in the set of light intensities corresponds to irradiation of photosensitive phase change cells in a grid space in the pixel division well.

[0030] According to an embodiment of the present application, the information to be stored includes an image to be stored.

[0031] The step of encoding the information to be stored according to the preset encoding principle to obtain the encoding set corresponding to the information to be stored specifically includes:

[0032] According to the distribution of the grid spaces in the pixel division well, the pixels of the image to be stored are segmented.

[0033] The average gray value of each pixel in the image to be stored is calculated, and the average gray value of each pixel is encoded according to the preset encoding principle to obtain the encoding set.

[0034] According to an embodiment of the present application, the step of obtaining a plurality of different light intensities based on the detection limit and the phase change sensitivity of the photosensitive phase change cells, and encoding the plurality of light intensities according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding specifically includes:

[0035] In the light intensity range included in the detection limit of the photosensitive phase change cells, the light intensity gradient is divided based on the phase change sensitivity of the photosensitive phase change cells, wherein the detection limit and the phase change sensitivity of the photosensitive phase change cells are obtained based on a pre-experiment.

[0036] In the light intensity range, a plurality of different light intensities are divided based on the light intensity gradient, and the plurality of light intensities are encoded according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding.

[0037] The electronic device according to the third aspect of the present application includes a memory, a processor, and a computer program stored in the memory and executable on the processor, and the processor executes the program to realize the information storage method of the cell phase change based photosensitive imaging device according to the second aspect of the present application.

[0038] The non-transitory computer readable storage medium according to the fourth aspect of the present application has a computer program stored thereon, and the computer program is executed by a processor to realize the information storage method of the cell phase change based photosensitive imaging device according to the second aspect of the present application.

[0039] Additional aspects and advantages of the present application will be made apparent from the following description. BRIEF DESCRIPTION OF DRAWINGS

[0040] In order to more clearly illustrate the technical solutions of the embodiments of the present application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiments or the prior art description. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without creative labor.

[0041] Figure 1 is one of the structure schematic diagram of the photosensitive phase change unit provided by the embodiments of the present application;

[0042] Figure 2 is the second structure schematic diagram of the photosensitive phase change unit provided by the embodiments of the present application;

[0043] Figure 3 is the step schematic diagram of the information storage method of the photosensitive imaging device provided by the embodiments of the present application;

[0044] Figure 4 is the structure schematic diagram of the electronic device provided by the embodiments of the present application.

[0045] Reference signs:

[0046] 1, photosensitive phase change cell; 2, photosensitive phase change cell slightly phase changing under weak light condition; 3, photosensitive phase change cell severely phase changing under strong light condition; 4, pixel division well; 5, environmental matrix; 6, packaging shell; 7, photosensitive performance enhancer. DETAILED DESCRIPTION

[0047] The embodiments of the present application will be further described in detail below in combination with the drawings and the embodiments. The following embodiments are used to illustrate the present application, but cannot be used to limit the scope of the present application.

[0048] In the description of the embodiments of the present application, it should be noted that the orientations or positional relationships indicated by the terms "center", "longitudinal", "transverse", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like are based on the orientations or positional relationships shown in the drawings, and are only for the convenience of describing the embodiments of the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the embodiments of the present application. In addition, the terms "first", "second", "third" are only for the purpose of description, and cannot be understood as indicating or implying relative importance.

[0049] In the description of the embodiments of the present application, it should be noted that unless specifically defined and limited otherwise, the terms "connected", "connected to", "connection" should be interpreted broadly, for example, can be fixed connection, can also be detachable connection, or integral connection; can be mechanical connection, can also be electrical connection; can be directly connected, can also be indirectly connected through intermediate medium. For those skilled in the art, the specific meaning of the above terms in the embodiments of the present application can be understood according to the specific circumstances.

[0050] In the embodiments of the present application, unless specifically defined and limited otherwise, the first feature is "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, the first feature "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or only indicates that the first feature is higher in horizontal height than the second feature. The first feature "below", "under" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or only indicates that the first feature is lower in horizontal height than the second feature.

[0051] In the description of the present specification, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" and the like means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the embodiments of the present application. In the present specification, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any appropriate manner in any one or more embodiments or examples. In addition, the skilled in the art can combine and combine the different embodiments or examples described in the present specification and the features of the different embodiments or examples without contradiction.

[0052] The cell phase change-based photosensitive imaging device according to the first aspect of the present application will be described below with reference to the accompanying drawings.

[0053] As shown in Figure 1 and Figure 2 The cell phase change-based photosensitive imaging device according to the embodiments of the present application includes a photosensitive phase change unit, a phase change detection unit and an image restoration unit.

[0054] The photosensitive phase change unit comprises a photosensitive phase change cell layer, an environmental matrix 5 and an encapsulation shell 6. The photosensitive phase change cell layer is composed of a plurality of photosensitive phase change cells 1. The photosensitive phase change cells 1 contain recombinant photosensitive phase change proteins. The photosensitive phase change cells 1 containing the recombinant photosensitive proteins will undergo phase change under the action of light. The photosensitive phase change cells 1 are cultured in the environmental matrix 5, so that the environmental matrix 5 can provide a suitable living environment for the photosensitive phase change cells 1 of the photosensitive phase change cell layer. The environmental matrix 5 and the photosensitive phase change cell layer are both encapsulated in the encapsulation shell 6, so that the encapsulation shell 6 can help the photosensitive phase change cells 1 resist interference from external adverse factors.

[0055] The phase change detection unit is used to detect the cell phase change information generated by the photosensitive phase change unit under the light intensity information of the target image. The image restoration unit internally stores the corresponding relationship between the cell phase change information and the light intensity under different exposure times. The image restoration unit is used to restore the cell phase change information detected by the phase change detection unit to the light intensity information, and convert the light intensity information to the gray scale information, so as to obtain the target image.

[0056] The imaging principle of the photosensitive imaging device based on cell phase change according to the embodiment of the present application is as follows: under the irradiation of light with intensity and wavelength within a certain range, the photosensitive area in the recombinant photosensitive phase change protein in the photosensitive phase change cell 1 will undergo aggregation, thereby inducing the aggregation of the recombinant photosensitive phase change protein and forming a second phase. The aggregation degree of the second phase is positively correlated with the light intensity and the light irradiation time of the original irradiation light within a certain range. For example Figure 2 As shown, the label 2 refers to the photosensitive phase change cell that undergoes slight phase change under weak light irradiation, and the label 3 refers to the photosensitive phase change cell that undergoes severe phase change under strong light irradiation. It can be seen that the phase change degree of the photosensitive phase change cell under weak light irradiation is greater than that of the photosensitive phase change cell under strong light irradiation.

[0057] The specific working process of the photosensitive imaging device is as follows: the photosensitive phase change unit is irradiated by the light intensity of the target image, the photosensitive phase change cells 1 in the photosensitive phase change unit undergo phase change, and then the phase change detection unit shoots the cell phase change image, so that the aggregation degree of the second phase (i.e. the cell phase change information) can be directly observed; the phase change detection unit transmits the cell phase change information to the image restoration unit, and then the image restoration unit restores the cell phase change information detected by the phase change detection unit to the light intensity information based on the corresponding relationship between the cell phase change information and the light intensity under different exposure times, and converts the light intensity information to the gray scale information, thereby obtaining the target image.

[0058] In conclusion, the photosensitive imaging device according to the present application realizes the photosensitive imaging function through the phase change characteristics of the photosensitive phase change cells 1 under the action of light, and since the photosensitive phase change cell layer in the device is based on natural life materials, the device can be reused without using silicon circuits, so that the device does not need to use chemical heavy metal products which are highly polluting, and is easy to degrade after use, so that the device is more green and environmentally friendly.

[0059] In the related art, the light conversion rate of the photosensitive part of the organism can reach the efficiency of one photon corresponding to the transmission of megaton ions, which is much higher than the theoretical photoelectric conversion limit of the current silicon-based sensor, and the biological material itself has the characteristics of good biocompatibility, green and non-polluting. Therefore, using biological materials to prepare a photosensitive device is a good solution to the dilemma of chemical photosensitive film and traditional photoelectric devices. The photosensitive biological materials currently explored and applied are mainly protein materials. For example, photosensitive imaging is performed by using the light cycle and light-driven proton pump effect of bacterial rhodopsin protein, or a photoelectric conversion device is constructed by using zinc-substituted cytochrome c and other electron transfer proteins.

[0060] However, since the working mode of the protein in the natural state is not completely clear at present, the natural or modified recombinant artificial photosensitive protein cannot achieve the working state in the cell in its natural state. In addition, when only using protein for photosensitive storage function, the protein needs to be irradiated with special waveband strong light to achieve a specific light cycle state, while the cell phase change can be carried out under relatively mild light.

[0061] Therefore, in order to solve the technical defects in the above related art, the present application uses a biological material at the level of cells to prepare a biological photosensitive imaging device by using the phase change characteristics, so as to further improve the imaging efficiency potential.

[0062] According to the photosensitive imaging device of the embodiment of the present application, the photosensitive phase change cell layer can be stored in the corresponding storage structure, and the storage structure is suitable for placing the environmental matrix 5 to provide a suitable living environment for the photosensitive phase change cells 1 in the photosensitive phase change cell layer. The storage structure is packaged in the packaging shell 6 after storing the photosensitive phase change cell layer and the environmental matrix 5. The present application does not specially limit the specific structure shape of the storage structure, as long as the storage structure is suitable for storing the photosensitive phase change cell layer and the environmental matrix 5.

[0063] The liquid-liquid phase transition in cells can be compared to the chemical changes produced by a light-sensitive film. In a natural state, the phase transition aggregation can remain stable on a time scale of hours or even longer, and the process can be controlled by incident light to control the degree of phase transition and reversibility. These characteristics make it possible to image using the phase transition characteristics of cells as a possible bio-material photosensitive solution. However, if a photosensitive device is to be prepared, since the cells themselves grow freely and some cells have aggregation, uniformity of imaging sensitivity is still a problem.

[0064] Therefore, in order to solve the technical problem of uniformity of imaging sensitivity, in one embodiment, as shown in Figure 1 and Figure 2 The photosensitive phase transition unit also includes a pixel division well 4, which is the storage structure described above. The pixel division well 4 is a well-shaped three-dimensional structure, and the pixel division well 4 forms a plurality of mutually independent (i.e., mutually isolated) grid spaces. It can be understood that each grid space corresponds to a pixel in an image, but it should be noted that, unlike a single pixel in an image, each grid space in the pixel division well 4 is a three-dimensional space with a well depth, thereby facilitating the movement of photosensitive phase transition cells 1 in a single grid space.

[0065] The photosensitive phase transition cells 1 of the photosensitive phase transition cell layer are uniformly distributed in each grid space, and each grid space is filled with an environmental matrix 5. The photosensitive phase transition cell layer, the environmental matrix 5, and the pixel division well 4 are encapsulated in an encapsulation shell 6.

[0066] The photosensitive phase transition unit is specifically used to convert the light intensity information of a target image into cell phase transition information of the photosensitive phase transition cells 1 in each grid space in the pixel division well 4. The phase transition detection unit is specifically used to detect the cell phase transition information of the photosensitive phase transition cells 1 in each grid space in the pixel division well 4. The image restoration unit is specifically used to restore the cell phase transition information of the photosensitive phase transition cells 1 in each grid space detected by the phase transition detection unit to the light intensity information at each grid space.

[0067] In this way, by separating the photosensitive phase transition cells 1 in the pixel division well 4 into a plurality of mutually independent grid spaces, migration of the photosensitive phase transition cells 1 during use can be prevented, forming relatively independent pixels, which is conducive to the realization of photosensitive imaging function. Further, the pixel division well 4 can be manufactured using two-photon photocuring and other additive technologies, so that the device achieves a resolution similar to existing advanced technologies, has a higher energy conversion efficiency than traditional silicon optical circuits and photosensitive imaging devices using biological molecules alone, and has the potential to further improve resolution.

[0068] The specific working process of the light-sensitive imaging device is as follows: in the imaging process, the light-sensitive phase change unit is assembled into an imaging device with a lens and a shutter, a target image is projected onto the light-sensitive phase change unit through the lens and the shutter, and after a certain exposure time, the light intensity information of the target image is converted into the cell phase change information of the light-sensitive phase change cell 1. The phase change detection unit detects the cell phase change information of the light-sensitive phase change cell 1 in each grid space in the pixel division well 4. The image restoration unit selects the corresponding relationship between the cell phase change information of the light-sensitive phase change cell 1 and the light intensity under the exposure time adopted in the actual imaging according to the exposure time adopted in the actual imaging, and then restores the cell phase change information of the light-sensitive phase change cell 1 in each grid space detected by the phase change detection unit to the light intensity information at each grid space according to the relationship, and then converts the light intensity information into gray scale information, and finally obtains the target image.

[0069] According to some embodiments of the present application, the environmental matrix 5 of the light-sensitive phase change unit includes one or more of gelatin, sodium alginate, mouse tumor-derived matrix gel, and fibrinogen, which are used for cell culture of the light-sensitive phase change cell 1.

[0070] According to some embodiments of the present application, the phase change detection unit can include a bright field microscope or a fluorescence microscope. By shooting the cell phase change image through the bright field or fluorescence microscope, the aggregation degree of the second phase can be observed through fluorescence or bright field aggregation, and the original light intensity can be restored according to the average gray scale value or the average fluorescence intensity per unit area.

[0071] In one embodiment of the present application, the recombinant light-sensitive phase change protein is a protein formed by combining the base sequences corresponding to the light-sensitive protein and the intrinsic disordered protein domain, and expressed by transfection into cells through a plasmid or a lentivirus as a carrier. The phase change detection unit includes a bright field microscope, and the specific method for detecting the cell phase change information of the light-sensitive phase change cell 1 is as follows: a bright field image is obtained by shooting the light-sensitive phase change cell 1 using the bright field microscope, and the average gray scale value per unit area of the phase change aggregation part of the light-sensitive phase change cell 1 in the bright field image is the cell phase change information.

[0072] In this embodiment, the plasmid vector is constructed in the following manner: a DNA sequence containing the sequences corresponding to the light-sensitive protein domain and the intrinsic disordered protein domain of the cell is synthesized, and then inserted into a circular plasmid through enzyme digestion to obtain the plasmid vector. The lentivirus vector is constructed in the following manner: a recombinant vector containing the sequences corresponding to the light-sensitive protein domain and the intrinsic disordered protein domain of the cell is synthesized, and the recombinant vector and a virus packaging plasmid are used to transfect host cells to perform virus packaging and production, and then virus liquid is collected and purified to obtain the lentivirus vector.

[0073] In another embodiment of the present application, the recombinant photosensitive phase change protein is a protein formed by combining the base sequences corresponding to the photosensitive protein domain, the fluorescent protein domain and the intrinsically disordered protein domain, and then expressing the protein in cells by using a plasmid or a lentivirus as a carrier. The phase change detection unit includes a fluorescence microscope, and the specific method for detecting the cell phase change information of the photosensitive phase change cell 1 is as follows: the photosensitive phase change cell 1 is photographed by using the fluorescence microscope to obtain a fluorescence image, and the unit area fluorescence intensity of the phase change aggregation part of the photosensitive phase change cell 1 in the fluorescence image is the cell phase change information.

[0074] In the present embodiment, the plasmid vector is constructed by synthesizing a DNA sequence containing the photosensitive protein domain, the fluorescent marker and the sequence corresponding to the intrinsically disordered protein domain, and then inserting the DNA sequence into a circular plasmid by enzyme digestion to obtain the plasmid vector. The lentivirus vector is constructed by synthesizing a recombinant vector containing the photosensitive protein domain, the fluorescent marker and the sequence corresponding to the intrinsically disordered protein domain, and then co-transfecting the host cells with the recombinant vector and a virus packaging plasmid to perform virus packaging and production, collecting and purifying the virus liquid to obtain the lentivirus vector.

[0075] According to an embodiment of the present application, the corresponding relationship between the cell phase change information of the photosensitive phase change cell 1 and the light intensity under different exposure times stored in the image restoration unit is determined by a pre-experiment, and the specific determination method is as follows: a calibration photosensitive phase change unit identical to the photosensitive phase change unit of the photosensitive imaging device is constructed, different intensity lights are respectively irradiated to the calibration photosensitive phase change unit, and after a certain exposure time, the cell phase change information of the photosensitive phase change cell 1 in the photosensitive phase change cell layer is detected by using the cell phase change detection device, the cell phase change information of the photosensitive phase change cell 1 under different light intensities at the exposure time is recorded, and the corresponding relationship between the two under the exposure time is determined by fitting; then the exposure time is changed, and the corresponding relationship between the cell phase change information of the photosensitive phase change cell 1 and the light intensity under different exposure conditions is determined by fitting in the same way.

[0076] According to some embodiments of the present application, the photosensitive protein includes Arabidopsis Cry2 protein or human ChR2 protein; and the intrinsically disordered protein domain includes FUS, DDX4 or HNRNPA1.

[0077] According to an embodiment of the present application, the photosensitive phase change cell layer of the photosensitive phase change unit further includes a photosensitive performance enhancer 7, which can improve the photoelectric response performance of the recombinant photosensitive phase change protein. The photosensitive performance enhancer 7 is made of a material capable of enhancing the intensity or modulating the wave band of the incident light, and specifically, the photosensitive performance enhancer 7 includes a photosensitive protein, up-conversion nanoparticles or metal nanoparticles independent of the photosensitive phase change cell 1.

[0078] According to one embodiment of the present application, the pixel division well 4 and the packaging shell 6 are both made of biocompatible materials by additive manufacturing methods. For example, the pixel division well 4 can be made by using a two-photon molding or a near-field electrospinning process, and the packaging shell 6 is made of a biocompatible material such as a photosensitive resin, PCL, or other materials that can be shaped by additive manufacturing methods.

[0079] According to some embodiments of the present application, the length and width of the single grid space of the pixel division well 4 ranges from 10 μm x 10 μm to 200 μm x 200 μm, and the depth of the single grid space ranges from 100 μm to 500 μm. Of course, the length and width of the single grid space and the depth of the single grid space can also use other size ranges, which are not specially limited in the present application.

[0080] In summary, the photosensitive imaging device according to the embodiments of the present application is based on natural life materials, does not need to use a combination of silicon circuits, can be reused, does not need to use a chemical heavy metal product with high pollution, and is easy to degrade after use, so it is environmentally friendly. In addition, the photosensitive sensitivity and the photosensitive range of the photosensitive phase change cell layer can be adjusted, the type of light-driven phase change photosensitive protein can be adjusted by adjusting the base fragments of the imported cells, so as to adjust the response range and degree of external light, and the response range and degree of external light can also be adjusted by adjusting the photosensitive performance enhancement material. In addition, the pixel division well 4 can prevent the migration of cells during use and form relatively independent pixels. The pixel division well 4 can be manufactured by using additive technologies such as two-photon photocuring, so as to achieve a resolution close to that of existing advanced technologies, and the energy conversion efficiency is higher than that of traditional silicon photo circuits and photosensitive imaging devices using only biological molecules, and has the potential to further improve the resolution.

[0081] Two embodiments of the photosensitive imaging device according to the present application are described below.

[0082] Embodiment one:

[0083] In the present embodiment, the phase change detection unit comprises a fluorescence microscope, and the preparation method of the photosensitive phase change unit is as follows: first, photosensitive phase change cells 1 containing recombinant photosensitive proteins are prepared: using cultured neural stem cells as tool cells, a lentiviral vector containing photosensitive protein FUS, fluorescent protein mcheery and intrinsic disordered protein domain Cry2 base sequence is constructed, the tool cells are transfected with the lentiviral vector to obtain photosensitive phase change cells 1. The cells after 72h of transfection are dissociated from the substrate by hydrolytic enzyme, and after centrifugation, the photosensitive phase change cell 1 cell suspension is obtained. The environmental matrix 5 composed of gelatin, sodium alginate and fibrinogen is mixed with the cell suspension of the photosensitive phase change cells 1, and then coated on the pixel division well 4 printed by the two-photon photocuring method. The silver nanoparticle sol is coated 0.5mm on the inner surface of the packaging shell 6, and is dried in a 70℃ oven to obtain the combination of the photosensitive performance enhancement 7 and the packaging shell 6. The combination of the photosensitive performance enhancement 7 and the packaging shell 6, the pixel division well 4 and the photosensitive phase change cell layer are assembled in a light-proof environment to obtain the photosensitive phase change unit.

[0084] The above-mentioned photosensitive phase change unit is assembled into an imaging device with a lens and a shutter, and the target image is projected onto the photosensitive phase change unit through the lens and the shutter. The shutter is used for shooting, and the light intensity information of the target image is converted into the cell phase change information of the photosensitive phase change cells 1 in each grid space in the pixel division well 4 after a certain exposure time.

[0085] After the shooting is completed, the photosensitive phase change unit is taken out, and the cell phase change information of the photosensitive phase change cells 1 in each grid space in the pixel division well 4 is observed by the fluorescence microscope in a light-proof manner. The specific method is as follows: using the fluorescence microscope, the photosensitive phase change cells 1 of the photosensitive phase change unit are excited by green light, the CCD of the fluorescence microscope is used to obtain the fluorescence image in the field of view, the whole negative image is spliced to obtain the fluorescence image of the whole negative image. The fluorescence image is analyzed, the phase change part in the single grid space in the pixel division well 4 is taken, and the average fluorescence intensity in the unit area is obtained by using the image processing software as the cell phase change value; the remaining grid spaces are operated in turn to obtain the cell phase change information in each grid space in the pixel division well 4.

[0086] The correspondence between the cell phase change information of the photosensitive phase change cell 1 and the light intensity at different exposure times stored in the image restoration unit is determined by a pre-experiment. The specific determination method is as follows: a calibration photosensitive phase change unit identical to the photosensitive phase change unit of the photosensitive imaging device is constructed, and is placed in a darkroom. The exposure time t is fixed. Different grid spaces in the pixel division well 4 of the calibration photosensitive phase change unit are irradiated with white light of different intensities of 0.01 μW, 0.1 μW, 0.5 μW, 1 μW, 10 μW, 100 μW and 1000 μW, respectively. Then, the average fluorescence intensity of the phase change part per unit area of each grid space is detected under light shielding conditions by using a fluorescence microscope, that is, the cell phase change value. The cell phase change values of the photosensitive phase change cell 1 under different light intensities are recorded, and a curve is fitted to obtain the preliminary correspondence between them. According to the curve trend, additional subdivided light intensity irradiation experiments are performed at positions with large slopes to obtain cell phase change values. Then, the curve is continuously fitted to obtain the correspondence between the cell phase change information of the photosensitive phase change cell 1 and the light intensity under the condition of the exposure time t. Then, the exposure time t is changed, and the correspondence between the cell phase change information of the photosensitive phase change cell 1 and the light intensity under different exposure times is determined in the same way.

[0087] Example Two

[0088] In this embodiment, the phase change detection unit includes a fluorescence microscope, and the photosensitive phase change unit is prepared as follows: first, the photosensitive phase change cell 1 containing a recombinant photosensitive protein is prepared: the cultured neural stem cells are used as tool cells, a slow virus vector containing the photosensitive protein FUS and the intrinsic disordered protein domain Cry2 base sequence is constructed, and the tool cells are transfected with the slow virus vector to obtain the photosensitive phase change cell 1. The cells after transfection for 72 h are dissociated from the substrate by hydrolytic enzyme, and the cell suspension of the photosensitive phase change cell 1 is obtained after centrifugation and resuspension. The environmental matrix 5 composed of gelatin, sodium alginate and fibrinogen is mixed with the cell suspension of the photosensitive phase change cell 1, which is coated on the pixel division well 4 obtained by two-photon photocuring printing, and the silver nanoparticle sol is coated on the inner surface of the packaging shell 6 by 0.5 mm. The combination of the photosensitive performance enhancer 7 and the packaging shell 6 is dried in a 70°C oven, to obtain the combination of the photosensitive performance enhancer 7 and the packaging shell 6. Finally, the combination of the photosensitive performance enhancer 7 and the packaging shell 6, the pixel division well 4 and the photosensitive phase change cell layer are assembled in a light shielding environment to obtain the photosensitive phase change unit.

[0089] The above photosensitive phase change unit is assembled into an imaging device with a lens and a shutter. The target image is projected onto the photosensitive phase change unit through the lens and the shutter, and is photographed by using the shutter. After exposure for a certain time, the light intensity information of the target image is converted into the cell phase change information of the photosensitive phase change cell 1 in each grid space of the pixel division well 4.

[0090] After the image was taken out, the photosensitive phase transition unit was removed, and the cell phase transition information of the photosensitive phase transition cell 1 in each grid space of the pixel division well 4 was observed using a bright-field microscope in the dark. The specific method is as follows: The cell phase transition information of the photosensitive phase transition cell 1 in each grid space of the pixel division well 4 was observed using a bright-field microscope. That is, the grayscale image in the field of view was obtained using the CCD of the bright-field microscope. The overall film image was stitched together to obtain the cell phase transition information in each grid space of the pixel division well 4.

[0091] The correspondence between the cell phase transition information of photosensitive phase transition cells 1 stored in the image restoration unit and the light intensity under different exposure times was determined through preliminary experiments. The specific determination method is as follows: A calibration photosensitive phase transition unit identical to the photosensitive phase transition unit of the photosensitive imaging device was constructed and placed in a dark room. With a fixed exposure time t, white light of different intensities (0.01 μW, 0.1 μW, 0.5 μW, 1 μW, 10 μW, 100 μW, and 1000 μW) was irradiated into different grid spaces of the pixel division well 4 of the calibration photosensitive phase transition unit. Then, under light-shielding conditions, the average gray value per unit area of ​​the phase transition part in each grid space was detected using a bright-field microscope, i.e., the cell phase transition value. The cell phase transition values ​​of photosensitive phase transition cells 1 under different light intensities were recorded, and a curve was fitted to obtain the preliminary correspondence between the two. Based on the curve trend, a supplementary subdivided light intensity irradiation experiment was conducted at positions with a larger slope to obtain the cell phase transition values. The curve was then fitted again to obtain the correspondence between the cell phase transition information of photosensitive phase transition cells 1 and the light intensity under an exposure time of t. Then, by changing the exposure time t, the correspondence between the cell phase transition information of photosensitive phase transition cell 1 and the light intensity was determined using the same method at different exposure times.

[0092] The present invention also protects an information storage method for a photosensitive imaging device based on cell phase transition. It should be noted that the above information storage method is implemented based on the photosensitive imaging device based on cell phase transition described in the first aspect of the present invention.

[0093] like Figure 3 As shown, the information storage method of the photosensitive imaging device based on cell phase transition according to the second aspect embodiment of the present invention includes:

[0094] Step 100: Based on the detection limit and phase transition sensitivity of the photosensitive phase transition cell 1, multiple different light intensities are obtained, and the multiple light intensities are encoded according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding; wherein, the detection limit and phase transition sensitivity of the photosensitive phase transition cell 1 are obtained based on preliminary experiments.

[0095] Step 200: Encode the information to be stored according to the preset encoding principle to obtain the encoding set corresponding to the information to be stored;

[0096] At step 300, the light intensity set corresponding to the code set is obtained according to the standard correspondence table.

[0097] At step 400, the photosensitive phase change cell 1 is irradiated by the light in the light intensity set to obtain the phase change information of the photosensitive phase change cell 1, and the information to be stored is stored as the phase change information of the photosensitive phase change cell 1, wherein each light intensity in the light intensity set corresponds to the photosensitive phase change cell 1 in a grid space in the pixel division well 4.

[0098] According to an embodiment of the present application, the information to be stored includes an image to be stored; and the step 200 of encoding the information to be stored according to a preset encoding principle to obtain the code set corresponding to the information to be stored specifically includes:

[0099] According to the distribution of the grid space in the pixel division well 4, the pixels of the image to be stored are segmented;

[0100] The average gray value of each pixel in the image to be stored is calculated, and the average gray value in each pixel is encoded according to the encoding principle to obtain the code set.

[0101] According to an embodiment of the present application, based on the detection limit and the phase change sensitivity of the photosensitive phase change cell 1, a plurality of different light intensities are obtained, and the plurality of light intensities are encoded according to the preset encoding principle to obtain the standard correspondence table between the plurality of light intensities and the plurality of codes.

[0102] Within the light intensity range included in the detection limit of the photosensitive phase change cell 1, the light intensity gradient is divided based on the phase change sensitivity of the photosensitive phase change cell 1, wherein the detection limit and the phase change sensitivity of the photosensitive phase change cell 1 are obtained based on pre-experiments;

[0103] Within the light intensity range, a plurality of different light intensities are divided based on the light intensity gradient, and the plurality of light intensities are encoded according to the preset encoding principle to obtain the standard correspondence table between the light intensity and the code.

[0104] It should be noted that the above-mentioned information to be stored can include text information, image information or sound information, etc., and the present application does not make special limitation here, as long as the information to be stored can be stored by using the above-mentioned information storage method.

[0105] In addition, the above-mentioned preset encoding principle can use various known encoding principles, such as binary, quaternary or decimal encoding principles, and the present application does not make special limitation here.

[0106] Two embodiments of the information storage method of the cell phase change based photosensitive imaging device according to the present application will be introduced below.

[0107] Embodiment three:

[0108] For step 200, taking the text information as an example of information to be stored, when encoding the text information, the existing GB2312 encoding BIG5 encoding and other binary encoding formats can be used, or an encoding system can be set in combination with the phase change sensitivity and detection limit of the phase change photoreceptor cell 1, for example, an 8-bit, 16-bit or other bit form can be directly used.

[0109] In one embodiment, if an 8-bit encoding method is used, the corresponding relationship between the light intensity and the encoding is shown in Table 1. Of course, Table 1 only serves as an example and does not constitute a specific limitation on the information storage method of the present application. The present application can also use other encoding methods to encode the text information.

[0110] Table 1 Encoding Table 1 of Example Three

[0111] Encoding Illumination intensity / μW (for example only) 0 1 1 100 2 200 3 300 4 400 5 500 6 600 7 700

[0112] In another embodiment, if the domestic 8-bit encoding method is used to encode the capital letters, i.e. one text corresponds to the information stored in two grid spaces, the corresponding relationship between the light intensity and the encoding is shown in Table 2. Of course, Table 2 only serves as an example and does not constitute a specific limitation on the information storage method of the present application. The present application can also use other encoding methods to encode the text information.

[0113] Table 2 Encoding Table 2 of Example Three

[0114]

[0115]

[0116] Example Four:

[0117] For step 200, taking the text information as an example of information to be stored, when encoding the text information, the existing GB2312 encoding BIG5 encoding and other binary encoding formats can be used, or an encoding system can be set in combination with the phase change sensitivity and detection limit of the phase change photoreceptor cell 1, for example, an 8-bit, 16-bit or other bit form can be directly used.

[0118] When storing the image, if the aspect ratio of the image to be stored is the same as the aspect ratio of the pixel division well 4, the image to be stored is evenly divided into 100x100 pixels; if the aspect ratio of the image to be stored is not the same as the aspect ratio of the pixel division well 4, the image to be stored is divided into as many pixels as possible according to the aspect ratio of the image to be stored and the number of pixels of the pixel division well 4, for example, if the pixel division well 4 is a square in each grid space, and the aspect ratio of the image to be stored is 1:2, the image to be stored is evenly divided into 100x50 pixels. After dividing the pixels, the average of the gray value in each pixel of the image to be stored is calculated, and the gradient is divided according to the range of the average gray value, and encoded.

[0119] If encoded in octal, the encoding table and the correspondence between the light intensity and the encoding are shown in Table 3 (only an example).

[0120] Table 3 Encoding table of Example 4

[0121]

[0122]

[0123] Figure 4 An example of a schematic diagram of the physical structure of an electronic device is shown in Figure 4 The electronic device can include a processor 810, a communications interface 820, a memory 830, and a communications bus 840, wherein the processor 810, the communications interface 820, and the memory 830 communicate with each other through the communications bus 840. The processor 810 can invoke the logical instructions in the memory 830 to execute the information storage method of the photosensitive imaging device described above.

[0124] In addition, the logical instructions in the memory 830 described above can be implemented in the form of a software functional unit and sold or used as a separate product, which can be stored in a computer-readable storage medium. Based on this understanding, the technical solutions of the present application essentially or the part that contributes to the prior art or part of the technical solutions can be embodied in the form of a software product, which is stored in a storage medium, includes a plurality of instructions to make a computer device (which can be a personal computer, a server, or a network device, etc.) execute all or part of the steps of the method described in various embodiments of the present application. The aforementioned storage medium includes: a U disk, a mobile hard disk, a read-only memory (ROM, Read-Only Memory), a random access memory (RAM, Random Access Memory), a magnetic disk or an optical disk, and various media that can store program codes.

[0125] Further, the embodiments of the present application disclose a computer program product, which comprises a computer program stored on a non-transitory computer readable storage medium, and the computer program comprises program instructions, and the computer is capable of performing the method provided by the above-mentioned method embodiments, such as the information storage method of the above-mentioned photosensitive imaging device, when the program instructions are executed by the computer.

[0126] In another aspect, the embodiments of the present application also provide a non-transitory computer readable storage medium, which stores a computer program, and the computer program is executed by a processor to implement the transmission method provided by the above-mentioned embodiments, such as the information storage method of the above-mentioned photosensitive imaging device.

[0127] The device embodiments described above are only schematic, and the units described as separate components may or may not be physically separate, and the components displayed as units may or may not be physical units, i.e. may be located in one place, or may be distributed on a plurality of network units. Part or all of the modules can be selected according to actual needs to achieve the purpose of the embodiments. Those skilled in the art can understand and implement without creative labor.

[0128] From the above description of the embodiments, those skilled in the art can clearly understand that the embodiments can be realized by means of software plus necessary universal hardware platforms, and of course can also be realized by hardware. Based on such understanding, the above technical solutions, essentially or in other words, the part of the prior art that makes a contribution, can be embodied in the form of a software product, which can be stored in a computer readable storage medium, such as ROM / RAM, magnetic disk, optical disk, etc., and includes a number of instructions to make a computer device (which can be a personal computer, a server, or a network device, etc.) execute the methods described in the various embodiments or some parts of the embodiments.

[0129] The above embodiments are only used to illustrate the present application, but not to limit the present application. Although the present application is explained in detail with reference to the embodiments, those skilled in the art should understand that various combinations, modifications or equivalent replacements of the technical solutions of the present application do not deviate from the spirit and scope of the present application, and should be covered in the scope of claims of the present application.

Claims

1. A photoreceptor device based on cell phase transition, characterized by, The application relates to a photoreceptor phase change unit, a phase change detection unit and an image restoration unit. The photoreceptor phase change unit comprises a photoreceptor phase change cell layer, an environment matrix and a packaging shell, the photoreceptor phase change cell layer comprises a plurality of photoreceptor phase change cells containing recombinant photosensitive phase change proteins, the photoreceptor phase change cells are suitable for phase change under light and for converting light intensity information into cell phase change information; the environment matrix is suitable for providing a suitable living environment for the photoreceptor phase change cells, and the environment matrix and the photoreceptor phase change cell layer are packaged in the packaging shell. The phase change detection unit is used for detecting cell phase change information generated by the photoreceptor phase change unit under light intensity information of a target image. The image restoration unit internally stores a corresponding relationship between cell phase change information and light intensity under different exposure times, the image restoration unit is used for restoring the cell phase change information detected by the phase change detection unit into light intensity information, and the light intensity information is converted into gray scale information to obtain the target image. The photoreceptor phase change unit further comprises pixel division wells, the pixel division wells are well-shaped three-dimensional structures, and the pixel division wells form a plurality of independent grid spaces. The photoreceptor phase change cells of the photoreceptor phase change cell layer are uniformly distributed in each grid space, each grid space is filled with the environment matrix, and the photoreceptor phase change cell layer, the environment matrix and the pixel division wells are packaged in the packaging shell. The photoreceptor phase change unit is specifically used for converting the light intensity information of the target image into cell phase change information of the photoreceptor phase change cells in each grid space of the pixel division well; the phase change detection unit is specifically used for detecting the cell phase change information of the photoreceptor phase change cells in each grid space of the pixel division well; and the image restoration unit is specifically used for restoring the cell phase change information of the photoreceptor phase change cells in each grid space detected by the phase change detection unit into light intensity information at each grid space. The recombinant photosensitive phase change protein is a protein formed by combining corresponding base sequences of a photosensitive protein, a fluorescent protein and an intrinsic disordered protein domain through plasmid or slow virus as a carrier and transfection into cells for expression construction. The phase change detection unit comprises a bright field microscope, the bright field microscope is used for photographing the photoreceptor phase change cells to obtain a bright field image, and the unit area average gray scale value of the phase change aggregation part of the photoreceptor phase change cells in the bright field image is the cell phase change information. The recombinant photosensitive phase change protein is a protein formed by combining corresponding base sequences of a photosensitive protein, a fluorescent protein and an intrinsic disordered protein domain through plasmid or slow virus as a carrier and transfection into cells for expression construction. The phase change detection unit comprises a fluorescent microscope, the fluorescent microscope is used for photographing the photoreceptor phase change cells to obtain a fluorescent image, and the unit area fluorescent intensity of the phase change aggregation part of the photoreceptor phase change cells in the fluorescent image is the cell phase change information.

2. A photonic imaging device based on cell phase transition according to claim 1, characterized in that, The corresponding relationship between the cell phase change information of the photoreceptor phase change cells and the light intensity under different exposure times stored in the image restoration unit is determined through a pre-experiment.

3. The photonic imaging device based on cell phase transition according to claim 1, wherein, The photosensitive protein comprises Arabidopsis Cry2 protein or human ChR2 protein, and the intrinsic disordered protein domain comprises FUS, DDX4 or HNRNPA1.

4. The photic imaging apparatus based on cell phase transition according to claim 1, wherein, The light-sensitive phase-change cell layer further comprises a light-sensitive performance enhancer; The light-sensitive performance enhancer comprises a light-sensitive protein, up-conversion nanoparticles or metal nanoparticles independent of the light-sensitive phase-change cell.

5. The photonic imaging device based on cell phase transition according to claim 1, wherein, Both the pixel division well and the packaging shell are prepared by an additive manufacturing method.

6. The photonic imaging device based on cell phase transition according to claim 1, wherein, The length and width of the single grid space of the pixel division well range from 10 μm×10 μm to 200 μm×200 μm, and the depth ranges from 100 μm to 500 μm.

7. An information storage method based on the cell phase-change based photoreceptive imaging apparatus according to any one of claims 1 to 6, characterized by, The method comprises: Based on the detection limit and phase-change sensitivity of the light-sensitive phase-change cell, a plurality of different light intensities are obtained, and the plurality of light intensities are encoded according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding; wherein the detection limit and phase-change sensitivity of the light-sensitive phase-change cell are obtained based on pre-experiments; The information to be stored is encoded according to the preset encoding principle to obtain an encoding set corresponding to the information to be stored; According to the standard correspondence table, a light intensity set corresponding to the encoding set is obtained; The light-sensitive phase-change cell is irradiated with light in the light intensity set to obtain the phase-change information of the light-sensitive phase-change cell, and the information to be stored is stored as the phase-change information of the light-sensitive phase-change cell, wherein each light intensity in the light intensity set corresponds to irradiation of the light-sensitive phase-change cell in a grid space in the pixel division well.

8. The information storage method of the cell phase transition-based photospotting device according to claim 7, characterized by, The information to be stored includes an image to be stored; The step of encoding the information to be stored according to the preset encoding principle to obtain an encoding set corresponding to the information to be stored specifically comprises: According to the distribution of the grid spaces in the pixel division well, the pixels of the image to be stored are segmented; The average gray value of each pixel in the image to be stored is calculated, and the average gray value in each pixel is encoded according to the preset encoding principle to obtain the encoding set.

9. The information storage method of the cell phase transition-based photospotting device according to claim 7, wherein The step of obtaining a plurality of different light intensities based on the detection limit and phase-change sensitivity of the light-sensitive phase-change cell, and encoding the plurality of light intensities according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding specifically comprises: Within the light intensity range included in the detection limit of the light-sensitive phase-change cell, a light intensity gradient is divided based on the phase-change sensitivity of the light-sensitive phase-change cell, wherein the detection limit and phase-change sensitivity of the light-sensitive phase-change cell are obtained based on pre-experiments; Within the light intensity range, a plurality of different light intensities are divided based on the light intensity gradient, and the plurality of light intensities are encoded according to a preset encoding principle to obtain a standard correspondence table between light intensity and encoding.

10. An electronic device comprising a memory, a processor, and a computer program stored on the memory and executable on the processor, characterized in that, The processor executes the program to implement the information storage method of the cell phase-change-based light-sensitive imaging device according to any one of claims 7 to 9. 11.A non-transitory computer-readable storage medium having stored thereon a computer program. The computer program is executed by the processor to implement the information storage method of the cell phase-change-based light-sensitive imaging device according to any one of claims 7 to 9.

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