Anti-oxidative stress nano dressing and preparation method thereof

Abstract

This invention, belonging to the field of biomedical materials, discloses an anti-oxidative stress nano-dressing and its preparation method. The invention involves self-assembling rare earth metal salt nanoparticles, a mercaptophenol compound, and superoxide dismutase to form anti-oxidative stress nanoparticles, and then electrospinning the resulting nano-dressing. The dressing prepared by the invention exhibits a potent anti-oxidative stress effect.

Description

Technical Field

[0001] The present invention belongs to the field of biomedical materials, and in particular relates to an anti-oxidative stress nano dressing and a preparation method thereof. Background Art

[0002] Chronic wounds in humans are known to harbor elevated levels of oxidative stress. Oxidative stress occurs when an imbalance of redox chemistries exists within the affected tissue. Reactive species are categorized into two main groups: reactive nitrogen species and reactive oxygen species. When oxidative free radicals accumulate within tissues, it is often due to inhibition or deficiency of detoxifying antioxidant enzymes. Consequently, improper regulation and response to oxidative stress results in significant damage to DNA, proteins, and lipids. Oxidative damage to DNA can lead to nucleotide oxidation and single- and double-strand breaks. Oxidative damage to amino acid residues can alter protein structure and ultimately their function within the cell. Given the known elevated levels of oxidative stress in chronic wounds, inhibiting oxidative stress within wounds could promote wound healing. Summary of the Invention

[0003] In view of this, the object of the present invention is to provide an anti-oxidative stress nano dressing and a preparation method thereof, wherein the dressing has good anti-oxidative stress effect.

[0004] In order to solve the above technical problems, the present invention provides an anti-oxidative stress nano dressing and a preparation method thereof, comprising the following steps:

[0005] S1. Rare earth metal salt nanoparticles, a thiolphenol compound, and superoxide dismutase were added to water, respectively, and ultrasonically treated in an ice bath with stirring to obtain an anti-oxidative stress nanoparticle suspension;

[0006] S2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step S1 to obtain anti-oxidative stress nanoparticle powder;

[0007] S3. The anti-oxidative stress nanoparticle powder prepared in step S2 is added to a DMF solution of TPU (thermoplastic polyurethane) and electrospun to obtain an anti-oxidative stress nano-dressing.

[0008] Preferably, in step S1, the rare earth metal salt is one of yttrium ferrite, strontium lanthanum manganate and strontium lanthanum cobalt ferrite.

[0009] Preferably, in step S1, the average particle size of the rare earth metal salt nanoparticles is 5-30 nm.

[0010] Preferably, in step S1, the mercaptophenol compound is one of 4-(3-mercaptopropyl)benzene-1,2-diphenol, 5-(1-mercaptoethyl)benzene-1,3-diphenol, 4-(1-mercaptoethyl)benzene-1,3-diphenol and 2-(1-mercaptoethyl)benzene-1,4-diphenol.

[0011] Preferably, in step S1, the specific activity of the superoxide dismutase is 6000-10000 U / g.

[0012] Preferably, in step S1, the ratio of the rare earth metal salt nanoparticles, the mercaptophenol compound, the superoxide dismutase and water is (0.1-0.2) g: (0.2-0.4) g: (0.4-0.8) g: 100 mL.

[0013] Preferably, in step S3, the concentration of the TPU DMF solution is 80-150 g / L.

[0014] Preferably, in step S3, the ratio of the antioxidant stress nanoparticle powder to the TPU DMF solution is 0.1 g: (100-300) mL.

[0015] Preferably, in step S3, the electrospinning conditions are voltage 10-13 kV, distance 10-14 cm, and injection rate 1-1.2 mL / h.

[0016] The present invention also provides an anti-oxidative stress nano dressing prepared by the above preparation method.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention forms a sulfhydryl self-assembled membrane on the surface of rare earth metal salt nanoparticles through sulfhydryl affinity. The phenolic hydroxyl group of the sulfhydrylphenol compound then anchors superoxide dismutase (SOD) to the surface of the nanoparticles, forming a catalytic center that effectively scavenges reactive oxygen free radicals. The presence of the phenolic hydroxyl group promotes the self-assembly coating of SOD and, through its reducing properties, stabilizes the active center of SOD. Under oxidative stress, the dressing, through the activation of the rare earth metal salt and the catalytic ability of the phenolic hydroxyl group of the sulfhydrylphenol compound and SOD, can efficiently scavenge free radicals, ultimately converting them into water and oxygen. Furthermore, compared to existing technologies, the use of nanofiber membranes as carriers increases the specific surface area for reaction and enhances the free radical scavenging rate. Because rare earth elements can synergize with transition elements, rare earth metal salts have higher chemical activity and redox balance regulation capabilities compared to conventional transition metal salts. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 Clear test results for DPPH. DETAILED DESCRIPTION

[0020] In order to further understand the present invention, preferred embodiments of the present invention are described below in conjunction with examples. However, it should be understood that these descriptions are only for further illustrating the features and advantages of the present invention, rather than for limiting the claims of the present invention.

[0021] Example 1

[0022] An anti-oxidative stress nano dressing and a preparation method thereof, comprising the following steps:

[0023] 1. Add 0.12 g of 25 nm average particle size lanthanum strontium cobalt ferrite nanoparticles, 0.31 g of 4-(3-mercaptopropyl)benzene-1,2-diol, and 0.54 g of superoxide dismutase with a specific activity of 8500 U / g to 100 mL of water, ultrasonically treat in an ice bath, and stir to obtain an anti-oxidative stress nanoparticle suspension.

[0024] 2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step 1 to obtain anti-oxidative stress nanoparticle powder;

[0025] 3. 0.1 g of the anti-oxidative stress nanoparticle powder prepared in step 2 was added to 220 mL of a DMF solution containing 120 g / L TPU and electrospun at a voltage of 12 kV, a distance of 12 cm, and an injection rate of 1.1 mL / h to obtain an anti-oxidative stress nanodressing.

[0026] Example 2

[0027] An anti-oxidative stress nano dressing and a preparation method thereof, comprising the following steps:

[0028] 1. Add 0.1 g of 5 nm average particle size strontium lanthanum manganate nanoparticles, 0.4 g of 5-(1-mercaptoethyl)benzene-1,3-diol, and 0.4 g of superoxide dismutase with a specific activity of 6000 U / g to 100 mL of water, ultrasonically treat in an ice bath, and stir to obtain an anti-oxidative stress nanoparticle suspension.

[0029] 2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step 1 to obtain anti-oxidative stress nanoparticle powder;

[0030] 3. 0.1 g of the anti-oxidative stress nanoparticle powder prepared in step 2 was added to 100 mL of DMF solution containing 80 g / L TPU and electrospun at a voltage of 13 kV, a distance of 14 cm, and an injection rate of 1.2 mL / h to obtain an anti-oxidative stress nanodressing.

[0031] Example 3

[0032] An anti-oxidative stress nano dressing and a preparation method thereof, comprising the following steps:

[0033] 1. Add 0.2 g of yttrium ferrite nanoparticles with an average particle size of 30 nm, 0.2 g of 4-(1-mercaptoethyl)benzene-1,3-diol, and 0.8 g of superoxide dismutase with a specific activity of 10,000 U / g to 100 mL of water, ultrasonically treat in an ice bath, and stir to obtain an anti-oxidative stress nanoparticle suspension.

[0034] 2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step 1 to obtain anti-oxidative stress nanoparticle powder;

[0035] 3. Add 0.1 g of the anti-oxidative stress nanoparticle powder prepared in step 2 to 300 mL of DMF solution containing 150 g / L TPU and perform electrospinning at a voltage of 10 kV, a distance of 10 cm, and an injection rate of 1 mL / h to obtain an anti-oxidative stress nanodressing.

[0036] Comparative Example 1

[0037] A dressing and a preparation method thereof, comprising the following steps:

[0038] 1. Add 0.12 g of 25 nm average particle size strontium cobalt ferrite lanthanum nanoparticles and 0.31 g of 4-(3-mercaptopropyl)benzene-1,2-diphenol to 100 mL of water, ultrasonicate in an ice bath, and stir to obtain an antioxidant nanoparticle suspension.

[0039] 2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step 1 to obtain anti-oxidative stress nanoparticle powder;

[0040] 3. 0.1 g of the antioxidant stress-resistant nanoparticle powder prepared in step 2 was added to 220 mL of a DMF solution containing 120 g / L TPU and electrospun at a voltage of 12 kV, a distance of 12 cm, and an injection rate of 1.1 mL / h to produce a nano-dressing.

[0041] Comparative Example 2

[0042] A dressing and a preparation method thereof, comprising the following steps:

[0043] 1. Add 0.12 g of 25 nm average particle size lanthanum strontium cobalt ferrite nanoparticles and 0.54 g of superoxide dismutase with a specific activity of 8500 U / g to 100 mL of water, ultrasonicate in an ice bath, and stir to obtain an anti-oxidative stress nanoparticle suspension.

[0044] 2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step 1 to obtain anti-oxidative stress nanoparticle powder;

[0045] 3. 0.1 g of the antioxidant stress-resistant nanoparticle powder prepared in step 2 was added to 220 mL of a DMF solution containing 120 g / L TPU and electrospun at a voltage of 12 kV, a distance of 12 cm, and an injection rate of 1.1 mL / h to produce a nano-dressing.

[0046] Comparative Example 3

[0047] A dressing and a preparation method thereof, comprising the following steps:

[0048] 1. Add 0.12 g of gold nanoparticles with an average particle size of 25 nm, 0.31 g of 4-(3-mercaptopropyl)benzene-1,2-diol, and 0.54 g of superoxide dismutase with a specific activity of 8500 U / g to 100 mL of water. Ultrasonicate and stir in an ice bath to obtain an anti-oxidative stress nanoparticle suspension.

[0049] 2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step 1 to obtain anti-oxidative stress nanoparticle powder;

[0050] 3. 0.1 g of the antioxidant stress-inhibiting nanoparticle powder prepared in step 2 was added to 220 mL of a DMF solution containing 120 g / L TPU and electrospun at a voltage of 12 kV, a distance of 12 cm, and an injection rate of 1.1 mL / h to prepare a dressing.

[0051] Antioxidant performance test

[0052] The degradation free radical levels of the anti-oxidative stress nano dressings prepared in Examples 1-3 and the dressings prepared in Comparative Examples 1-3 were determined by DPPH scavenging method. The higher the DPPH scavenging rate, the better the anti-oxidative stress effect. Figure 1 .

[0053] according to Figure 1 The results show that the anti-oxidative stress nano-dressings prepared in Examples 1-3 have good DPPH free radical scavenging effects. The dressings prepared in Comparative Examples 1-3 have poor DPPH free radical scavenging effects. The reason for this is that in Comparative Example 1, there is no catalytic effect of superoxide dismutase, and the free radical scavenging effect is also poor. In Comparative Example 2, there is no anchoring of the mercaptophenol compound to superoxide dismutase, and the phenolic hydroxyl group does not affect the active center of superoxide dismutase, resulting in poor free radical scavenging effect. Comparative Example 3 uses gold nanoparticles, whose chemical activity and redox capacity are inferior to those of rare earth metal salts, and their free radical scavenging ability is also reduced.

[0054] The present invention provides an anti-oxidative stress nano-dressing and a method for preparing the same. Numerous methods and approaches exist for implementing this technical solution. The foregoing merely represents a preferred embodiment of the present invention. It should be noted that those skilled in the art may make various improvements and modifications without departing from the principles of the present invention, and such improvements and modifications are also within the scope of protection of the present invention. Any components not specified in this embodiment may be implemented using existing technologies.

Claims

1. A method for preparing an anti-oxidative stress nano dressing, characterized in that: The steps include: S1. Rare earth metal salt nanoparticles, a mercaptophenol compound, and superoxide dismutase were added to water, ultrasonically treated in an ice bath and stirred to obtain an antioxidant stress nanoparticle suspension, wherein the amount of the rare earth metal salt nanoparticles, the mercaptophenol compound, superoxide dismutase, and water was 0.12 g: 0.31 g: 0.54 g: 100 mL; The rare earth metal salt nanoparticles are strontium lanthanum cobalt ferrite nanoparticles; The mercaptophenol compound is 4-(3-mercaptopropyl)benzene-1,2-diphenol; The specific activity of the superoxide dismutase is 8500 U / g; S2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step S1 to obtain anti-oxidative stress nanoparticle powder; S3. Add the anti-oxidative stress nanoparticle powder prepared in step S2 to the DMF solution of TPU and perform electrospinning to obtain an anti-oxidative stress nano-dressing.

2. A method for preparing an anti-oxidative stress nano dressing, characterized in that: The steps include: S1. Rare earth metal salt nanoparticles, thiol phenol compounds and superoxide dismutase were added to water, ultrasonically treated in an ice bath and stirred to obtain an antioxidant stress nanoparticle suspension, the amount of the rare earth metal salt nanoparticles, thiol phenol compounds, superoxide dismutase and water ratio of 0.1g: 0.4g: 0.4g: 100mL; The rare earth metal salt nanoparticles are strontium lanthanum manganate nanoparticles; The mercaptophenol compound is 5-(1-mercaptoethyl)benzene-1,3-diphenol; The specific activity of the superoxide dismutase is 6000 U / g; S2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step S1 to obtain anti-oxidative stress nanoparticle powder; S3. Add the anti-oxidative stress nanoparticle powder prepared in step S2 to the DMF solution of TPU and perform electrospinning to obtain an anti-oxidative stress nano-dressing.

3. A method for preparing an anti-oxidative stress nano dressing, characterized in that: The steps include: S1. Rare earth metal salt nanoparticles, thiol phenol compounds and superoxide dismutase were added to water, ultrasonically treated in an ice bath and stirred to obtain an antioxidant stress nanoparticle suspension, the amount of the rare earth metal salt nanoparticles, thiol phenol compounds, superoxide dismutase and water ratio of 0.2g: 0.2g: 0.8g: 100mL; The rare earth metal salt nanoparticles are yttrium ferrite nanoparticles; The mercaptophenol compound is 4-(1-mercaptoethyl)benzene-1,3-diphenol; The specific activity of the superoxide dismutase is 10000 U / g; S2. freeze-drying the anti-oxidative stress nanoparticle suspension prepared in step S1 to obtain anti-oxidative stress nanoparticle powder; S3. Add the anti-oxidative stress nanoparticle powder prepared in step S2 to the DMF solution of TPU and perform electrospinning to obtain an anti-oxidative stress nano-dressing.

4. The preparation method according to any one of claims 1 to 3, characterized in that In step S1, the average particle size of the rare earth metal salt nanoparticles is 5-30 nm.

5. The preparation method according to any one of claims 1 to 3, characterized in that In step S3, the concentration of TPU in the DMF solution of TPU is 80-150 g / L.

6. The preparation method according to any one of claims 1 to 3, characterized in that In step S3, the ratio of the antioxidant stress nanoparticle powder to the TPU DMF solution is 0.1 g: (100-300) mL.

7. The preparation method according to any one of claims 1 to 3, characterized in that In step S3, the electrospinning conditions are voltage 10-13 kV, distance 10-14 cm, and injection rate 1-1.2 mL / h.

8. The anti-oxidative stress nano dressing prepared according to the preparation method according to any one of claims 1 to 3.

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