Cosmetic agents, delivery of compositions and uses thereof

Abstract

The present invention provides new methods and systems, schemes and cosmetic compositions for repairing or reducing skin changes. The present invention relates to cosmetic compositions, which include electrospun polymer fibers, at least one active ingredient and at least one cosmetically acceptable carrier, wherein the electrospun fibers are dispersed in the composition. The composition is in the form of a solution, suspension, lotion, cream, gel, emulsion, toner, ointment, paste, foam, hydrogel, film-forming product or facial skin mask. The composition includes at least one active ingredient and further includes an antioxidant, a moisturizer, a surfactant or a wetting agent.

Description

[0001] field

[0002] The present invention generally relates to methods, systems, and cosmetic or dermatological compositions for repairing and reducing skin imperfections using polymeric fibers, including microfibers and nanofibers.

[0003] background

[0004] Aging, exposure to adverse environmental factors, pollutants, lack of good nutrition, fatigue, can affect the visual appearance, physical properties or physiological functions of the skin. These factors may produce a visually undesirable appearance to the skin. Significant changes in the skin include, for example, changes in the eye area, such as venous dilation, eye bags, dark circles and swelling around the eyes. Changes in other areas of the face include, for example, fine lines and wrinkles, loss of elasticity, loss of hardness, loss of uniformity of color or tone, rough surface texture, age spots and reduced moisture content. Many such changes in the appearance and function of the skin are caused by changes in the outer epidermal layer of the skin, while other changes are caused by changes in the lower dermis.

[0005] Polymer-based nanofibers are used as implantable drug delivery vehicles in the pharmaceutical industry. However, due to their high cost and low productivity, the application of such materials in cosmetic products is limited. In addition, with the development of electrospinning technology, production costs and scale-up capabilities have increased significantly in recent years.

[0006] It is therefore an object of the present invention to utilize polymer microfibers or nanofibers for cosmetic delivery systems, compositions and uses thereof.

[0007] Overview

[0008] One aspect of the present invention relates to a cosmetic composition, providing an electrospun polymer fiber, at least one active ingredient and at least one cosmetically acceptable carrier, wherein the electrospun fiber is dispersed in the composition. The composition is in the form of a solution, suspension, lotion, cream, gel, emulsion, suspension, toner, ointment, paste, foam, hydrogel, film-forming product or facial skin mask. The composition comprises at least one active ingredient selected from the following: cosmetic agent, peptide, DNA, RNA, polymer, protein, vitamin, organic acid, enzyme, oil and mixture thereof. The composition further comprises an antioxidant, a moisturizer, a surfactant or a wetting agent. The electrospun polymer hydrophobic fiber is selected from polycarbothane, polyvinyl acetate, polysulfone, polyvinyl chloride, polylactide (PLA), polyethylene, polystyrene, polyvinyl chloride, polytetrafluoroethylene, polydimethylsiloxane, polyurethane, polylactic acid, polytetrafluoroethylene, cellulose acetate and mixtures thereof. The fibers are present in an amount of about 0.01 wt % to about 10 wt % relative to the total weight of the composition, and further comprise a diameter of about 0.5 nm to about 0.5 μm.

[0009] Another aspect of the present invention relates to a method for reducing skin imperfections or improving the appearance of a subject's skin. The method comprises topically applying a composition comprising electrospun hydrophobic fibers and at least one active ingredient to the skin of a subject in need thereof. The method improves venous dilation, eye bags, dark circles and swelling around the eyes, fine lines and wrinkles, loss of elasticity, wrinkles, loss of hardness, loss of uniformity of color or tone, rough surface texture, age spots, or moisture content in the skin. The method further comprises applying the composition at least once a day, including in the morning and before going to bed. The method further comprises using the composition as a treatment regimen to achieve changes in the skin.

[0010] Another aspect of the present invention relates to a system or kit comprising a composition comprising electrospun fibrous hydrophobic fibers, at least one active ingredient and a cosmetically acceptable carrier. The system or kit further comprises instructions for use. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1A and Figure 1B Shown are the percentages of penetration of AA2G of the formulations from the skin model (Franz Cell) permeation study.

[0013] Figure 2A and 2B Shown is the permeability of AA2G in skin PAMPA studies.

[0014] Figure 2C Shown are the results of a skin permeation study starting at the 3 hour time point.

[0015] Figure 2D Comparative results of six-hour penetration of AA2G between the skin PAMPA study and the skin penetration study results are shown.

[0016] Figure 3 A permeation study showing caffeine in a chopped fiber formulation over 24 hours (Skin Permeation (FranzCell)).

[0017] Figure 4A and 4B Results are shown, demonstrating the permeability of caffeine in a skin PAMPA study.

[0018] Figure 4C Shown are the results of skin model penetration (Franz Cell) of caffeine with chopped fibers.

[0019] Figure 5 Shown are the results of the penetration of hydrophobic active substances and chopped fibers over 24 hours by skin penetration studies (Franz Cell).

[0020] Figure 6 Showing the penetration of hydrophobic actives obtained by skin PAMPA study.

[0021] Detailed Description

[0022] To facilitate understanding of the present invention, several terms are defined below. The terms defined herein have meanings that are commonly understood by those of ordinary skill in the art to which the present invention relates. Terms such as "a", "an" and "the" are not intended to refer only to singular entities, but to general categories that include specific examples thereof that may be used for illustration. The terms herein are used to describe specific embodiments of the present invention, but their use does not delimit the present invention unless outlined in the claims.

[0023] The term "active ingredient" or "active agent" or "cosmetic agent" refers to a cosmetic agent used to deliver a benefit to the skin. An "active ingredient" or "active agent" or "cosmetic agent" will cause, drive a change in the subject's skin or deliver the benefit in question, thereby helping to achieve a desired, expected or desired result. The term "active ingredient" or "active agent" or "cosmetic agent" according to the present invention includes cosmetically acceptable excipients or carriers that may be present in the composition / formulation.

[0024] The terms "prevent" and "preventing" include preventing the recurrence, spread or onset of a skin or hair condition. The present invention is not intended to be limited to complete prevention.

[0025] The term "subject" refers to any mammal, preferably a human.

[0026] The term "topical" refers to the administration of one or more agents (eg, cosmetics, vitamins, etc.) to the skin.

[0027] The terms "transdermal" or "topical" refer to the delivery of an agent (eg, cosmetic, dermatological, vitamin, etc.) through the skin (eg, such that at least some portion of a population of particles reaches the underlying layers of the skin).

[0028] The term "hydrophilic" refers to the physical property of a molecule that is capable of transiently associating with water, ie, bonding to water via hydrogen bonds. The term "hydrophobic" refers to the physical property of a molecule that repels large amounts of water.

[0029] The term "solvent" refers to a liquid, solid or gaseous solute that produces a solution.

[0030] When used in the claims and / or specification, the terms "inhibit," "reduce," or "prevent" or any variation of these terms include any measurable decrease or complete inhibition to achieve the desired result.

[0031] As used in the specification and / or claims, the term "effective" means sufficient to accomplish a desired, intended, or desired result.

[0032] The term microfiber refers to fibers having a diameter greater than 1000 nm.

[0033] The term nanofiber refers to fibers with a diameter less than 1000 nm.

[0034] Except in the operating and comparative examples, or where otherwise explicitly indicated, all numbers in this specification indicating amounts or ratios of materials or reaction conditions, physical properties of materials and / or use are to be understood as modified by the word "about". Unless otherwise indicated, all amounts are expressed as percentages by weight of the final cosmetic.

[0035] The present invention relates to methods of repairing human skin using polymer electrospun fibers (including microfibers and nanofibers). In some embodiments, the present invention relates to compositions for repairing skin using polymer electrospun nanofibers. As the skin ages or is subjected to environmental or aging-related damage, measurable changes occur in the skin. Such damage causes a general decrease in cell and tissue viability, a decrease in cell replication rate, a decrease in skin blood flow, a decrease in moisture content, errors in structure and function, changes in biochemical pathways, and a decrease in the skin's ability to remodel and repair itself.

[0036] As a non-limiting example, the human skin around the periorbital area (i.e., around the eyes) is thin and delicate. The periorbital area is networked with tiny capillaries, and blood sometimes leaks from these capillaries, causing dark circles to appear. Other known causes of dark circles include UV exposure (e.g., exposure to sunlight can increase natural melanin levels and attract melanin to the skin surface, making it darker), aging (e.g., with age, the skin around the eyes can become even thinner, which makes dark circles more obvious), fatigue (fatigue can make the skin paler, which makes dark circles look darker), allergies (e.g., allergic reactions can cause stains in the under-eye area, and conditions that cause people to rub their eyes can make dark circles worse, because scratching or rubbing can darken the skin), pregnancy or menstruation (e.g., during pregnancy and menstruation, the skin becomes pale, which makes dark circles look darker) and malnutrition.

[0037] As another non-limiting example, a condition in which the skin under the eyes swells and becomes visually undesirable is referred to as eye puffiness. Eye puffiness can be caused by several factors, including increased vascularization, leaky capillaries, thinning / loosening of the skin that can fill with more fluid, loss of fat pads under the eyes that can contribute to eye bags, and allergies, dust, and pollutants that can trigger the release of chemicals that swell the tissue around the eyes.

[0038] The method for treating or repairing skin includes stimulating the dermis or epidermis with many cosmetic active ingredients. Using such reagents can renew the skin cell rate and cause basal cell division. Several methods have been utilized to prevent, reduce or treat the damage to the skin (especially eyes) caused by environmental factors, chemicals, pollutants or aging. So far, most methods involve delivering one or more reagents to act on the skin to cause an effect. The example of such reagent includes a retinoid that stimulates collagen and an instrument that stimulates epidermal renewal, such as a film or a patch that is impregnated or carries one or more active agents in the patch.

[0039] According to one aspect of the present invention, compositions and methods for treating the appearance of human skin are provided, wherein such compositions comprise water-insoluble and essentially hydrophobic polymer microfibers or nanofibers. The applicants of the present invention surprisingly discovered that the presence of insoluble and hydrophobic polymer nanofibers in cosmetic formulations greatly enhances the penetration of skin care active ingredients.

[0040] It is reported that soluble hydrophilic polymer nanofibers (e.g., PVA) enhance the penetration of active ingredients. For example, water-soluble PVP nanofibers enhance the penetration of hydrophobic active substances into human skin. The applicant of the present invention surprisingly found that hydrophobic and water-insoluble polymer nanofibers enhance the penetration of one or more active ingredients.

[0041] In the field of cosmetic formulations, it is traditionally known that the use of hydrophobic materials in formulations will hinder the cosmetic benefits and manufacturing methods that can be used in the process of preparing the composition. Therefore, the general method relies on the use of water-soluble and hydrophilic materials.

[0042] The inventors of the present application have found that the use of hydrophobic nanofibers does not prevent the delivery of active ingredients into the skin. On the contrary, the hydrophobic nanofibers greatly enhance the penetration of active agents into the skin. They have also found that the addition of water-insoluble polymer nanofibers serves as an occlusive layer that assists and enhances skin hydration. In addition, mainly due to the soft and flexible nature of the hydrophobic nanofibers, the sensory sensation of the occlusive layer enhances the penetration of active ingredients into the skin.

[0043] Thus, one aspect of the present invention comprises a topical cosmetic composition comprising electrospun hydrophobic polymer fibers dispersed in the composition. The composition may also comprise any amount of hydrophilic polymer fibers within the composition. The composition further comprises a cosmetically active ingredient and a carrier.

[0044] In some embodiments, polymer microfibers or nanofibers include, but are not limited to, polycarbothane (aliphatic polycarbonate-based TPU), Shore A 75 to Shore D 72, poly(vinyl acetate), polysulfone poly(vinyl chloride), biodegradable polylactide (PLA), polyethylene, polystyrene, polyvinyl chloride, polytetrafluoroethylene, polydimethylsiloxane, polyester, polyurethane, acrylic resin, epoxy resin, polylactic acid, polytetrafluoroethylene, polyketal, cellulose acetate. In a preferred embodiment, polymer nanofibers are used, including cellulose acetate.

[0045] In some embodiments, the composition can include electrospun hydrophilic polymer fibers of any shape or size dispersed in the formulation. Hydrophilic polymers include, but are not limited to, poly(ethylene glycol), poly(propylene glycol), poly(vinyl alcohol), polypyrrolidone or polyvinylpyrrolidone (PVP) and biodegradable polyactives (soft polyethylene glycol-terephthalate block copolymers and hard polybutylene terephthalate), etc. The polymer fibers are electrospun fibers.

[0046] Any type of skin care active can be used and is contemplated within the disclosure of the present invention. The skin care active can be hydrophobic, hydrophilic or amphiphilic. The skin care active can be a small molecule, a lipid, a peptide, a DNA molecule, a biomolecule, an enzyme or a mixture thereof. In order to better disperse the polymer nanofibers, the nanofibers are pre-cut or chopped into a predetermined length and present in the composition as dispersed fibers, also referred to as "dispersed" in the composition in the present disclosure.

[0047] In a preferred embodiment, the nanofibers are dispersed in the composition and exist as chopped fibers with a diameter ranging from about 0.5 nm to about 5 μm. In other embodiments, the nanofibers comprise a diameter ranging from about 5 nm to 1000 nm. In a preferred embodiment, the nanofibers comprise a diameter ranging from about 0.05 μm to 0.5 μm. The length of the fibers (as a dispersion, in the form of chopped fibers) is about 0.1-10 mm. In some embodiments, the length is about 1-3 mm. All ranges and sub-ranges are expected to be within the subject matter of the present invention. In the composition, the nanofibers are present at about 0.01 wt % to about 10 wt % relative to the total weight of the composition.

[0048] According to an embodiment, the cosmetic composition may include, but is not limited to, one or more of a DNA repair enzyme, a sunscreen active, a humectant, a plant extract, a peptide, an oil, a thickener, a surfactant, a vitamin, an antioxidant, a preservative, or a carrier. When present in the composition, the carrier is dermatologically or cosmetically acceptable.

[0049] The composition may be formulated as a cosmetic product in a cosmetic carrier in the form of an emulsion, cream, lotion, gel, serum, solution, spray, base or foam.

[0050] In one embodiment, the present invention contemplates a liquid composition for use on the skin, which comprises polymer electrostatically spun hydrophobic microfibers or nanofibers. According to an embodiment, the composition comprising polymer electrostatically spun hydrophobic microfibers or nanofibers further comprises an active ingredient, a cosmetic agent or a cosmetically acceptable carrier or excipient or a softener. In one embodiment, the composition further comprises hydrophobic polymer fibers (including microfibers or nanofibers). The polymer fibers are porous, soft and flexible. The hydrophobic polymer fibers are dispersed in the composition. They can be present in the composition as a dispersion in the form of chopped fibers of any shape, orientation or size.

[0051] Applicants of the present invention have unexpectedly and surprisingly achieved cosmetic benefits of improved skin appearance using the compositions disclosed herein. The compositions provide significant benefits, showing reductions in venous dilation, eye bags, dark circles and swelling around the eyes, fine lines and wrinkles, loss of elasticity, wrinkles, loss of hardness, loss of uniformity of color or tone, rough surface texture, age spots, and reduction of moisture content in the skin around the eyes and on the face. Additional benefits obtained by the compositions include, but are not limited to, using the compositions as antioxidants, collagen promoters, and lightening dark spots, smoothing wrinkles, promoting healing, and / or reducing scars.

[0052] The compositions of the present invention can be used with other modes of delivery, including microneedles, iontophoresis, and / or electroporation. For example, in one embodiment, microneedles are applied to the skin, and the composition is then applied. Any and all combinations and permutations of intended uses are part of the present invention.

[0053] The compositions of the present invention can be formulated as cosmetic products. In some embodiments, the compositions can be formulated to have a pH in the range of about 1-10. In some embodiments, the compositions can be formulated to have a pH of less than about 3.0, 3.5, 4.0, 4.1, 4.2, 4.3, 4.4, 4.5, 4.6, 4.7, 4.8, 4.9, 5.0, 5.1, 5.2, 5.3, 5.4, 5.5, 5.6, 5.7, 5.8, 5.9, 6.0, 6.1, 6.2, 6.3, 6.4, 6.5, 6.6, 6.7, 6.8, 6.9, 7.0, 7.1, 7.2, 7.3, 7.4, 7.5, 7.6, 7.7, 7.8, 7.9, 8.0, 8.1 , 8.2, 8.3, 8.4, 8.5, 8.6, 8.7, 8.8, 8.9, 9.0, 9.1, 9.2, 9.3, 9.4, 9.5, 9.6, 9.7, 9.8, 9.9, 10.0, 10.1, 10.2, 10.3, 10.4, 10.5, 10.6, 10.7, 10.8, 10.9, 11.0, 11.1, 11.2, 11.3, 11.4, 11.5, 11.6, 11.7, 11.8, 11.9 to about 12.0 or more, or any range or integer derivable therein.

[0054] The composition can be formulated into a cosmetic product in a cosmetic carrier as an emulsion, cream, lotion, gel, serum, solution, substrate, spray or foam. In some embodiments, the composition can be formulated for use more than once, twice, three times, four times a day. In a preferred embodiment, the composition can be formulated for use once, twice or more a day. In a more preferred embodiment, the composition can be formulated for use in the morning and before going to bed at night.

[0055] In the composition, the polymer microfibers (or nanofibers) comprise a hydrophobic polymer. In a further preferred embodiment, the polymer nanofibers comprise a hydrophobic polymer including cellulose acetate.

[0056] The composition according to the present invention is not limited by the properties of the one or more polymers used for the microfibers (or nanofibers). Any type of polymer that is hydrophobic and water-insoluble can be used. In some embodiments, multiple (different) polymers can be used together or separately. The present invention is also not limited by the properties of the biomaterial.

[0057] In one embodiment, the composition comprising polymer hydrophobic microfibers or nanofibers further comprises one or more active ingredients. The active ingredient can be incorporated into the formulation, or can be incorporated into the fiber by impregnation into the dispersed fiber. In some embodiments, the active ingredient can be incorporated into the fiber during electrospinning.

[0058] Another aspect of the present invention is a topical delivery system. In one embodiment, the topical delivery system comprises at least one skin care active ingredient and polymer microfibers or nanofibers in a cosmetic composition. The polymer microfibers or nanofibers are electrospun, water-insoluble and hydrophobic, and are present in the composition as a dispersion in the form of chopped fibers. The system may further include a treatment regimen, instructions on how to use the composition of the present invention with any other similar cosmetic composition or application.

[0059] Another aspect of the present invention is the use of compositions comprising hydrophobic electrospun polymer microfibers or nanofibers for personal use, including cosmetic applications of human subjects. In some embodiments, the compositions can be used as skin care agents. In some other embodiments, the compositions can be used as agents for treating or preventing skin conditions. In some embodiments, the compositions can be used as cleansers, exfoliants, or skin repair agents.

[0060] On the other hand, a method for improving, treating, repairing or reducing the visual appearance of the skin is provided. The method comprises topically administering an effective amount of a composition comprising an electrostatically spun polymer hydrophobic nanofiber comprising an active ingredient or a cosmetic agent. The present invention relates to a method for repairing or improving skin, the method comprising administering the skin care composition by providing a skin care composition according to the present invention to a human subject and by applying or contacting the subject's skin. In embodiments, the composition is in liquid form. In other embodiments, the composition is topically or transdermally administered. In some embodiments, the composition can be applied once or more than once in a given day. In some embodiments, the composition can be applied before going to bed at night.

[0061] In particular, the composition can be used to prevent or treat skin changes such as venous dilatation, eye bags, dark circles and swelling around the eyes, fine lines and wrinkles, loss of elasticity, wrinkles, loss of firmness, loss of uniformity of color or tone, rough surface texture, age spots, and reduced moisture content in the skin. In particular, the present invention relates to a method for repairing or reducing venous dilatation, eye bags, dark circles, swelling around the eyes, fine lines and wrinkles, loss of elasticity, wrinkles, loss of firmness, tightening, firming, loss of uniformity of color or tone, rough surface texture, age spots, and reduced moisture content of the skin around the eyes and the skin of the face.

[0062] In other embodiments, the subject shows symptoms related to the affected visual appearance, the physical properties of the skin or physiological functions (such as the visually undesirable appearance of the skin) or suspects that there is an affected visual appearance, the physical properties of the skin or physiological functions, which include possible venous dilation, eye bags, dark circles and swelling around the eyes, environmental damage to the skin, fine lines and wrinkles, elasticity loss, wrinkles, loss of hardness, loss of uniformity of color or tone, rough surface texture, age spots and moisture content reduction. Topical application of the composition can treat or prevent such skin conditions. The effectiveness of the composition can be compared with the skin not treated or processed with the composition of the present invention. In some non-limiting embodiments, skin treatment can be located in the region (such as eyes) and / or its surroundings where the composition is applied to the skin. The skin can be face, trunk, back, neck, ear, pelvis, arm, hand, leg (such as, ankle, knee, thigh), foot or buttocks skin. The non-limiting examples of skin conditions that can be treated or prevented with the compositions of the present invention include telangiectasia (i.e. spider veins), eye circles (e.g., dark circles), eye swelling, itching, freckles, age spots, senile purpura, keratosis, melasma, rash spots, wrinkles, fine lines, nodules, sunburned skin, acne or hyperpigmentation. In some aspects, skin conditions can be caused by exposure to UV light, aging, radiation, long-term sunlight exposure, environmental pollutants, air pollution, wind, cold, heat, chemicals, disease pathology or smoking. Skin to be treated can be aging, malnourished or environmentally damaged skin. In some aspects, the composition can be topically applied with an amount that effectively increases the stratum corneum renewal rate of the skin, the collagen synthesis of the skin, the fat production of the skin, the firmness of the skin or the elasticity of the skin. In other aspects, the composition can be topically applied with an amount that effectively reduces or suppresses the formation of new capillaries in or near the skin, the blood flow of the skin, the amount of fluid in or near the skin or the melanin production in the skin.

[0063] In embodiments, the present invention contemplates polymeric hydrophobic fibers, such as cellulose acetate nanofibers, cellulose acetate microfibers, combinations of the two. In preferred embodiments, the polymeric fibers are chopped and dispersed in small sizes, with diameters of about 5 nm to about 1000 nm, more preferably about 5 nm to 500 nm. The fibers may be dispersed in the composition.

[0064] Also disclosed are systems or kits that can include the compositions of the present invention. In certain non-limiting aspects, the composition is contained in a container. The container can be a bottle, a dispenser, a package, etc. The container can be configured to dispense a predetermined amount of the composition. The container can be configured to dispense the composition in the form of a liquid, a spray, an emulsion, or an aerosol. In certain aspects, the system or kit can include instructions on its surface and / or instructions for using the composition.

[0065] In other aspects of the present invention, compositions can be used as a part of the scheme for treating skin conditions. For example, the scheme can include applying the compositions of the present invention in the first case, such as disclosed throughout this specification. The scheme can then include applying the same, similar or different other applications as the first case. Additional application can include, for example, using the compositions of the present invention and / or another method (such as, other compositions, etc.) for treating specific skin conditions for the second, third, fourth, fifth, sixth, seventh, eighth, ninth, tenth or more times. The scheme can also include applying the compositions before going to bed in the morning and / or evening.

[0066] Active ingredients and forms

[0067] According to one aspect of the present invention, the cosmetic composition may include, but is not limited to, one or more of a DNA repair enzyme, a sunscreen active, a humectant, a plant extract, a peptide, an oil, a thickener, a surfactant, a vitamin, an antioxidant, a preservative, or a carrier. If present, the suggested range is about 0.0001-35%, preferably about 0.0005-20%, more preferably about 0.001-15%. When present in the composition, the carrier is dermatologically or cosmetically acceptable.

[0068] In some embodiments, the composition is a liquid composition.The composition can be formulated into a cosmetic product in a cosmetic carrier as an emulsion, cream, lotion, gel, serum, solution, base or foam.

[0069] According to one embodiment, the preparation comprising the cosmetic agent can be applied to mammalian keratinous tissue, human skin, face or hair. The preparation comprising the cosmetic agent can be in various forms. For example, some non-limiting examples of such forms include solutions, suspensions, lotions, creams, gels, emulsions, suspensions, toners, ointments, cleansers, exfoliants, liquid shampoos and hair conditioners, pastes, foams, powders, mousses, shaving creams, hydrogels, film-forming products, face and skin masks, etc.

[0070] The formulation type of the cosmetic agent of the present invention may be any type including a solution system, a soluble system, an emulsion system, a gel system, a powder dispersion system or a water-oil two-phase system.

[0071] The composition can be in the form of an aqueous solution, gel, cream, lotion, emulsion, slurry, spray or suspension. Emulsions can be water-in-oil or oil-in-water. The composition can also be anhydrous. The composition can be in liquid, semi-solid or solid form.

[0072] If the composition is present as an aqueous solution or dispersion, the amount of water present may range from about 0.01-99%, and the amount of dissolved or dispersed solids from about 10-99.99%. If the composition of the present invention is present in the form of an emulsion, it may contain about 0.1-99% water and about 0.1-80% oil. If the composition of the present invention is in an anhydrous form, it may contain about 0.1-99% oil.

[0073] Conventional cosmetic adjuvants suitable as additives are, for example, co-emulsifiers, fats and waxes, stabilizers, thickeners, biogenic agents, film formers, fragrances, dyes, pearlescent agents, preservatives, pigments, electrolytes (e.g., magnesium sulfate) and pH regulators. Co-emulsifiers are preferably known W / O and O / V emulsifiers, such as polyglycerol esters, sorbitan esters or partially esterified glycerides. Typical examples of fats are glycerides; waxes mentioned as combinations of hydrophilized len-growing waxes, especially beeswax, paraffin or microcrystalline wax. Metal salts of fatty acids, such as magnesium stearate, aluminum stearate and / or zinc stearate, can be used. Suitable thickeners are, for example, cross-linked polyacrylic acid and its derivatives, polysaccharides, more particularly xanthan gum, guar gum, agar, alginates and tyloses, carboxymethyl cellulose and hydroxyethyl cellulose, as well as fatty alcohols, monoglycerides and fatty acids, polyacrylates, polyvinyl alcohol and polyvinyl pyrrolidone. Conventional film formers are, for example, hydrocolloids, such as chitosan, microcrystalline chitosan or quaternized chitosan, polyvinylpyrrolidone, vinylpyrrolidone-vinyl acetate copolymers, polymers of the acrylic acid series, quaternized cellulose derivatives and similar compounds. Suitable preservatives are, for example, formaldehyde solutions, parabens or sorbic acid. For example, coldistearat pearlizing agents (e.g., ethylene glycol distearate), but also fatty acids and fatty acids are contemplated. Dyes suitable for cosmetic purposes and approved materials can be used. Such dyes are usually used in a concentration of 0.001-0.1% by weight, based on the total mixture.

[0074] Thus, the composition of the present invention may be in liquid, paste or solid form, such as water-in-oil creams, oil-in-water creams and lotions, aerosol foam creams, gels, oils, grease pens, powders, sprays or hydroalcoholic lotions. The composition may include any active ingredient or cosmetic agent and a cosmetically acceptable excipient or carrier.

[0075] DNA repair enzymes

[0076] The composition may also contain one or more DNA repair enzymes. The DNA repair enzyme may be present in an amount of about 0.00001% to about 35%, preferably about 0.00005% to about 30%, more preferably about 0.0001% to about 25% of one or more DNA repair enzymes.

[0077] DNA repair enzymes such as those disclosed in U.S. Pat. Nos. 5,077,211; 5,190,762; 5,272,079; and 5,296,231 are suitable for use in the compositions and methods of the invention described herein. An example of such a DNA repair enzyme is available from AGI / Dermatics under the trade name It is available for purchase and has the INCI name Arabidopsis Thaliana Extract. It can be present alone or mixed with lecithin and water. This DNA repair enzyme is known to be effective in repairing 8-oxo-diguanine base mutation damage.

[0078] Another class of DNA repair enzymes that can be used is an enzyme known to effectively repair 06-methylguanine base mutation damage. It is marketed by AGI / Dermatics under the trade name It is marketed and has the INCI name Lactobacillus ferment, which can be added to the composition of the invention by itself or mixed with lecithin and water.

[0079] Another class of DNA repair enzymes that can be used are enzymes known to effectively repair TT dimers. The enzymes are present in a mixture of biological or plant materials. Examples of such compositions are sold by AGI / Dermatics under the trade name or Sale. A mixture containing micrococcal lysate (the end product of controlled lysis of various micrococci), lecithin and water. A mixture comprising a plankton extract (which is an extract of marine biomass including one or more of the following organisms: plankton of the genus Algae, green microalgae, diatoms, green blue and nitrogen-fixing algae), water and lecithin.

[0080] Another class of DNA repair enzymes can be components of various inactivated bacterial lysates, such as Bifidobacterium (Bifida) lysate or Bifidobacterium fermentation lysate, which is a lysate of Bifidobacterium that contains metabolites and cytoplasmic fractions when Bifidobacterium is cultured, inactivated and then split. The INCI name for this material is Bifidobacterium fermentation lysate.

[0081] Sunscreen

[0082] The composition of the present invention may include one or more sunscreen actives or sunscreens. Examples of suitable sunscreen actives include oil-soluble sunscreens, insoluble sunscreens and water-soluble sunscreens. Non-limiting examples of suitable oil-soluble sunscreens are disclosed in The Cosmetic, Toiletry, and Fragrance Association's The International Cosmetic Ingredient Dictionary and Handbook, 10th edition, Gottschalck, TE and McEwen, Jr. editors (2004), pages 2267 and 2292-93, and include benzophenone-3, bis-ethylhexyloxyphenol methoxyphenyl triazine, butyl methoxydibenzoylmethane, diethylamino hydroxybenzoyl hexyl benzoate, drometrizole trisiloxane, ethylhexyl methoxycinnamate, ethylhexyl salicylate, ethylhexyl triazone, octocrylene, homosalate, polysilicone-15 and derivatives thereof and mixtures. Non-limiting examples of suitable insoluble sunscreens include methylene bis-benzotriazolyl tetramethylbutyl-phenol, titanium dioxide, cerium zinc oxide, zinc oxide and derivatives and mixtures thereof. Non-limiting examples of suitable water-soluble sunscreens include phenylbenzimidazole sulfonic acid (PBSA), terephthalylidene dicamphor sulfonic acid (Mexoryl TM SX), benzophenone-4, benzophenone-5, benzyl camphor sulfonic acid, cinnamic acid aminopropyl trimethyl ammonium chloride, methoxycinnamic acid aminopropyl ethyl dimethyl ammonium chloride ether, diethylphenyl triamino triazine stilbene disulfonic acid disodium, distilbene biphenyl disulfonic acid disodium, phenyl dibenzimidazole tetrasulfonic acid disodium, methoxycinnamic acid aminopropyl hydroxysulfobetaine, methoxycinnamic acid aminopropyl lauryl dimethyl toluene sulfonic acid, PEG-25PABA (para-aminobenzoic acid), polyquaternium-59, TEA-salicylate and its salts, derivatives and mixtures. All known sunscreen actives are considered to be within the scope of the present invention.

[0083] Wetting agent

[0084] The composition may contain one or more wetting agents. If present, the wetting agent may be in the range of about 0.1-75%, preferably about 0.5-70%, more preferably about 0.5-40%. Examples of suitable wetting agents include, but are not limited to, glycols, sugars, and the like. Suitable glycols are in monomeric or polymeric form and include polyethylene glycol and polypropylene glycol, such as PEG 4-10, which is polyethylene glycol having 4-10 repeating ethylene oxide units; and C 1-6Alkylene glycols, such as propylene glycol, butylene glycol, pentylene glycol, etc. Suitable sugars are also suitable humectants, some of which are also polyols. Examples of such sugars include glucose, fructose, honey, hydrogenated honey, inositol, maltose, mannitol, maltitol, sorbitol, sucrose, xylitol, xylose, etc. Urea is also suitable. Preferably, the humectant used in the composition of the present invention is C 1-6 (Preferred C 2-4 ) alkylene glycols, most especially butylene glycol, glycerol, propylene glycol or hexylene glycol.

[0085] Plant Extracts

[0086] It may be desirable to incorporate one or more plant extracts into the composition. If present, a suggested range is from about 0.0001-20%, preferably from about 0.0005-15%, more preferably from about 0.001-10%. Suitable plant extracts include, but are not limited to, extracts from plants (herbs, roots, flowers, fruits, seeds) such as flowers, fruits, vegetables, etc., including yeast fermentation extract, Padina Pavonica extract, Thermus Thermophilis fermentation extract, Camelina Sativa seed oil, Boswelllia Serrata extract, Olive extract, Acacia Dealbata extract, Acer Saccharinum (Sugar Maple), Acidopholus, Acorus, Aesculus, Agaricus, Agave, Agrimonia, Algae, Aloe, Citrus, Brassica, Cinnamon, Orange, Apple, Blueberry, Cranberry, Peach, Pear, Lemon, Lime, Pea, Seaweed, Caffeine, Green Tea, Chamomile, Willow Bark, Mulberry, Poppy, and CTFA Cosmetic Those set forth in Ingredient Handbook, 8th Edition, Volume 2, pages 1646-1660.Additional specific examples include, but are not limited to, Glycyrrhiza Glabra, Salix Nigra, Macrocycstis Pyrifera, Pyrus Malus, Saxifraga Sarmentosa, Vitis Vinifera, Morus Nigra, Scutellaria Baicalensis, Anthemis Nobilis, Salvia Sclarea, Prunus Amygdalus, Rosmarinus Officianalis, Sapindus makurossi, Caesalpinia spinosa, Citrus Medica Limonum, Panax Ginseng, Siegesbeckia Orientalis, Mangifera Indicia, Fructus Mume, Psidium Guajava, Ascophyllum Nodosum, Centauriumerythrea, Wild Soybean (Glycine Soja) Extract, Beet (Beta Vulgaris), Haberlea Rhodopensis, Polygonum Cuspidatum, Sweet Lime (Citrus Aurantium Dulcis), Grape (Vitis Vinifera), Selaginella (Selaginella Tamariscina), Hops (Humulus Lupulus), Citrus (Citrus Reticulata) Peel, Pomegranate (Punica Granatum), Asparagopsis, Turmeric (Curcuma Longa), Menyanthes Trifoliata, Sunflower (Helianthus Annuus), Barley (Hordeum Vulgare), Cucumis Sativus, Evernia Prunastri, Evernia Furfuracea, Kola Acuminata, Glycyrrhetinic Acid and mixtures thereof.

[0087] Peptides

[0088] It may be desirable to incorporate one or more peptides into the composition. The term "peptide" refers to a biomolecule having about 2-20 amino acids linked by peptide bonds. The preferred range of peptides present in the composition is about 0.001-20%, preferably about 0.005-15%, more preferably about 0.01-10%. Preferred bioactive peptides include those described in CTF International Cosmetic Ingredient Dictionary and Handbook, 11th edition, 2006, page 2712. Such peptides include, but are not limited to, the CTFA names: acetyl hexapeptide-1, 7, 8; acetyl pentapeptide-1, 2, 3 or 5; acetyl tripeptide-1; acetyl dipeptide-1 hexadecyl ester; acetyl glutamyl heptapeptide-3; acetyl glutamyl hexapeptide-6; acetyl monofluoropeptide-1; heptapeptide-1, 2 or 3; hexapeptide-1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13 or 14; manganese tripeptide-1; myristoyl hexapeptide-5, 12 or 13; myristoyl Nonapeptide-2; Myristoyl pentapeptide-4; Myristoyl tetrapeptide-4 or 6; Myristoyl tripeptide-4; Nisin, nonapeptide-1 or 2; Oligopeptide-1, 2, 3, 4, 5, 6, 7, 8, 9 or 10; Palmitoyl hexapeptide-14; Palmitoyl pentapeptide-4; Palmitoyl pentapeptide-4 or 5; Palmitoyl tripeptide-1 or 5; Pentapeptide-1, 2, 3, 4, 5 or 6; Tetrapeptide-1, 2, 3, 4, 5, 6 or 7; Tripeptide-1, 2, 3, 4 or 5; or Palmitoyl oligopeptide. All peptides having cosmetic or dermatological applications are considered within the scope of the present invention.

[0089] In a preferred embodiment, the composition comprises acetyl hexapeptide-8, which is marketed under the trade name

[0090] Oil

[0091] The composition may also include one or more oils in the form of natural, synthetic or silicone oils. The term "oil" refers to an ingredient that is pourable at room temperature (e.g., 25°C). The oil may be volatile or non-volatile. The term "volatile" refers to an oil having a vapor pressure of greater than about 2 mm of mercury at 20°C. The term "non-volatile" refers to an oil having a vapor pressure of less than about 2 mm of mercury at 20°C. If present, the recommended range is about 0.1-60%, preferably about 0.5-45%.

[0092] Examples of volatile oils include volatile linear, cyclic or branched silicones such as cyclopentasiloxane, cyclohexasiloxane (2 cst), hexamethyldisiloxane (0.65 cst, centistokes), octamethyltrisiloxane (1.0 cst), decamethyltetrasiloxane (1.5 cst) or dodecamethylpentasiloxane (2.0 cst); or branched volatile silicones such as methyltrimethylpolysiloxane (1.5 cst). Volatile paraffins are also suitable, such as isododecane, isohexadecane, C11-14 alkanes and mixtures thereof.

[0093] Non-volatile oils include straight chain silicones commonly referred to as dimethylpolysiloxanes; phenyl-substituted silicones, such as phenyl dimethylpolysiloxane, phenyl trimethylpolysiloxane, trimethylsiloxyphenyl dimethylsiloxane, cetyl dimethylpolysiloxane, perfluorodimethylpolysiloxane, phenethyl dimethylpolysiloxane, etc. Non-volatile oils can also include esters or hydrocarbons. Esters include C1-10 alkyl esters of C1-20 carboxylic acids. A preferred ester type is a fatty acid (C6-22) ester of a straight or branched saturated or unsaturated C1-22 alkyl. Examples include esters with low viscosity (e.g., 10-100cst at room temperature). Examples of such esters include, but are not limited to, jojoba esters. Other non-volatile oils include sterols, such as phytosterols, phytosphingosine, and similar phytosterols.

[0094] Thickener

[0095] Suitable thickeners may be incorporated into the composition. Suitable thickeners may be present in the range of about 0.0001-45%, preferably about 0.0005-40%.

[0096] Examples of thickeners include animal waxes, vegetable waxes, mineral waxes, silicone waxes or synthetic waxes having a melting point in the range of about 30°C to 150°C, including but not limited to waxes made by Fischer-Tropsch synthesis, such as polyethylene waxes or synthetic waxes or various vegetable waxes, such as bayberry wax, candelilla wax, ozokerite, gum arabic, beeswax, ceresin, cetyl esters, flower waxes, citrus waxes, carnauba wax, jojoba wax, Japan wax, polyethylene, microcrystalline wax, rice bran, lanolin wax, mink wax, montan wax, bayberry wax, cocoa wax, ozokerite, palm kernel wax, paraffin, avocado wax, apple wax, shellac wax, sage wax, spent grain wax, grape wax and polyalkylene glycol derivatives thereof, such as PEG6-20 beeswax, or PEG-12 carnauba wax or fatty acids or fatty alcohols, including esters thereof, such as hydroxystearic acid (e.g. 12-hydroxystearic acid), tristearin, tribehenin, and the like.

[0097] In addition, suitable thickeners can be used in the composition, such as silicon dioxide, silicates, silylated silicon dioxide and alkali metal or alkaline earth metal derivatives thereof. These silicon dioxide and silicates are usually present in particulate form and can include silicon dioxide, silylated silicon dioxide, magnesium aluminum silicate, and the like.

[0098] Silicone elastomers may also be used as thickeners. Such elastomers include those formed by addition reaction-curing by reacting a SiH-containing diorganosiloxane and an organopolysiloxane or α-ω diene having terminal olefinic unsaturation in the presence of a platinum metal catalyst. Such elastomers may also be formed by other reaction methods, such as condensation-curing organopolysiloxane compositions by dehydrogenation reaction between a hydroxyl-terminated diorganopolysiloxane and a SiH-containing diorganopolysiloxane or α-ω diene in the presence of an organotin compound, or condensation-curing organopolysiloxane compositions using a condensation reaction between a hydroxyl-terminated diorganopolysiloxane and a hydrolyzable organosiloxane in the presence of an organotin compound or titanate; peroxide-cured organopolysiloxane compositions, which are thermally cured in the presence of an organic peroxide catalyst.

[0099] One type of elastomer that may be suitable is an addition reaction-cured organopolysiloxane having at least 2 lower alkenyl groups or α-ω dienes in each molecule and an organopolysiloxane having at least 2 silicon-bonded hydrogen atoms in each molecule; and a platinum type catalyst. Although the lower alkenyl groups (e.g., vinyl groups) may be present anywhere in the molecule, terminal olefinic unsaturation at one or both molecular ends is preferred. The molecular structure of this component may be linear, branched linear, cyclic, or network. Examples of these organopolysiloxanes are methylvinylsiloxane, methylvinylsiloxane-dimethylsiloxane copolymers, dimethylvinylsiloxy-terminated dimethylpolysiloxane, dimethylvinylsiloxy-terminated dimethylsiloxane-methylphenylsiloxane copolymers, dimethylvinylsiloxy-terminated dimethylsiloxane-diphenylsiloxane-methylvinylsiloxane copolymers, trimethylsiloxy-terminated dimethylsiloxane-methylvinylsiloxane copolymers, trimethylsiloxy-terminated dimethylsiloxane-methylphenylsiloxane-methylvinylsiloxane copolymers, dimethylvinylsiloxy-terminated methyl(3,3,3-trifluoropropyl)polysiloxane and dimethylvinylsiloxy-terminated dimethylsiloxane-methyl(3,3,3-trifluoropropyl)siloxane copolymers, decadiene, octadiene, heptadiene, hexadiene, pentadiene or butadiene or propadiene.

[0100] Curing occurs by addition reaction of silicon-bonded hydrogen atoms in dimethylmethylhydrogensiloxane with siloxane or α-ω diene using the catalysts mentioned herein. In order to form a highly crosslinked structure, the methylhydrogensiloxane must contain at least 2 silicon-bonded hydrogen atoms per molecule to optimize the function as a crosslinker.

[0101] The catalyst used in the addition reaction of silicon-bonded hydrogen atoms and alkenyl groups is specifically exemplified by chloroplatinic acid, which may be dissolved in an alcohol or ketone and the solution optionally aged, chloroplatinic acid-olefin complexes, chloroplatinic acid-alkenylsiloxane complexes, chloroplatinic acid-diketone complexes, platinum black and supported platinum.

[0102] Examples of suitable silicone elastomers for use in the compositions of the present invention may be in powder form, or dispersed or dissolved in a solvent such as a volatile or non-volatile silicone or a vehicle compatible with silicone such as an alkane or ester. Examples of silicone elastomer powders include vinyl dimethicone / methicone silsesquioxane crosspolymers such as Shin-Etsu's KSP-100, KSP-101, KSP-102, KSP-103, KSP-104, KSP-105, hybrid silicone powders containing fluoroalkyl groups such as Shin-Etsu's KSP-200, which is a fluoro-silicone elastomer, and hybrid silicone powders containing phenyl groups such as Shin-Etsu's KSP-300, which is a phenyl-substituted silicone elastomer; and Dow Corning's DC9506. Examples of silicone elastomer powders dispersed in a silicone compatible vehicle include dimethicone / vinyl dimethicone crosspolymers supplied by a number of suppliers, including Dow Corning Corporation under the trade names 9040 or 9041, GE Silicones under the trade name SFE 839, or Shin-Etsu Silicones under the trade names KSG-15, 16, 18. The CTFA name for KSG-15 is cyclopentasiloxane / dimethicone / vinyl dimethicone crosspolymer. The INCI name for KSG-18 is phenyl trimethicone / dimethicone / phenyl vinyl dimethicone crosspolymer. Silicone elastomers can also be purchased from Grant Industries under the trademark Gransil. Silicone elastomers having long chain alkyl substitution are also suitable, such as the lauryl dimethicone / vinyl dimethicone crosspolymers supplied by Shin Etsu under the trade names KSG-31, KSG-32, KSG-41, KSG-42, KSG-43, and KSG-44. Crosslinked organopolysiloxane elastomers useful in the present invention and methods for their preparation are further described in U.S. Pat. No. 4,970,252, issued Nov. 13, 1990 to Sakuta et al.; U.S. Pat. No. 5,760,116, issued Jun. 2, 1998 to Kilgour et al.; U.S. Pat. No. 5,654,362, issued Aug. 5, 1997 to Schulz, Jr. et al.; and Japanese Patent Application JP 61-18708 assigned to Pola Kasei Kogyo KK.

[0103] Polysaccharides can be suitable aqueous phase thickeners. Examples of such polysaccharides include materials of natural origin, such as agar, agarose, alicaligenes polysaccharide, algin, alginic acid, gum arabic, pullulan, chitin, dextran, cassia gum, cellulose gum, gelatin, gellan gum, hyaluronic acid, hydroxyethylcellulose, methylcellulose, ethylcellulose, pectin, sclerotium gum, xanthan gum, pectin, trehalose, gelatin, etc.

[0104] Different types of synthetic polymer thickeners are also suitable. One type includes acrylic polymer thickeners comprising monomers A and B, wherein A is selected from acrylic acid, methacrylic acid, and mixtures thereof; and B is selected from C1-22 alkyl acrylates, C1-22 alkyl methacrylates, and mixtures thereof. Acrylic polymer solutions include those sold by Seppic, Inc. under the trade name Those sold or under the trade name Those that are sold.

[0105] Also suitable are acrylic polymer thickeners which are copolymers of A, B and C monomers, wherein A and B are as defined above, and C has the general formula:

[0106]

[0107] Where Z is -(CH2) m ; wherein m is 1-10, n is 2-3, o is 2-200, and R is C 10-30 Straight or branched chain alkyl. Examples of such secondary thickeners are copolymers wherein A and B are as defined above, and C is CO, and wherein n, o and R are as defined above. Examples of such secondary thickeners include acrylates / steareth-20 methacrylate copolymers sold by Rohm & Haas under the trade name Acrysol ICS-1.

[0108] Anionic amphiphilic polymers based on acrylates containing at least one hydrophilic unit and at least one allyl ether unit containing a fatty chain are also suitable. Preference is given to those in which the hydrophilic unit contains an ethylenically unsaturated anionic monomer, more particularly a vinyl carboxylic acid (e.g. acrylic acid, methacrylic acid or a mixture thereof), and in which the allyl ether unit containing a fatty chain corresponds to a monomer of the formula:

[0109] CH2=CR'CH2OB n R

[0110] wherein R' represents H or CH3, B represents ethyleneoxy, n is zero or an integer in the range of 1-100, R represents a hydrocarbon group selected from alkyl, arylalkyl, aryl, alkylaryl and cycloalkyl groups containing 8-30 carbon atoms (preferably 10-24, and even more particularly 12-18 carbon atoms). More preferably in this case wherein R' represents H, n is equal to 10 and R represents a stearyl (C18) group. Anionic amphiphilic polymers of this type are described and prepared in U.S. Patent Nos. 4,677,152 and 4,702,844. In these anionic amphiphilic polymers, the polymer is formed from 20-60 wt % of acrylic acid and / or methacrylic acid, 5-60 wt % of lower alkyl methacrylate, 2-50 wt % of allyl ether containing a fatty chain as mentioned above and 0-1 wt % of a crosslinking agent, which is a well-known copolymerizable multiethylenically unsaturated monomer, such as diallyl phthalate, allyl (meth)acrylate, divinylbenzene, (poly)ethylene glycol dimethacrylate and methylenebisacrylamide. A commercial example of such a polymer is a crosslinked terpolymer of methacrylic acid, ethyl acrylate, polyethylene glycol ether of stearyl alcohol (with 10 EO units) or Steareth-10, in particular those sold under the names SALCARE SC80 and SALCARE SC90 by the company Allied Colloids, which are aqueous emulsions containing 30% of a crosslinked terpolymer of methacrylic acid, ethyl acrylate and Steareth-10 allyl ether (40 / 50 / 10).

[0111] Acrylate copolymers are also suitable, such as polyacrylate-3, which is a copolymer of methacrylic acid, methyl methacrylate, methyl styrene isopropyl isocyanate, and PEG-40 behenate monomers; polyacrylate-10, which is a copolymer of sodium acryloyldimethyl taurate, sodium acrylate, acrylamide, and vinyl pyrrolidone monomers; or polyacrylate-11, which is a copolymer of sodium acryloyldimethyl taurate, sodium acrylate, hydroxyethyl acrylate, lauryl acrylate, butyl acrylate, and acrylamide monomers.

[0112] Crosslinked acrylate-based polymers are also suitable, where one or more of the acrylic acid groups may have a substituted long chain alkyl group (e.g., 6-40, 10-30, etc.), for example, acrylates / C10-30 alkyl acrylate crosspolymers, which are copolymers of C10-30 alkyl acrylates and one or more monomers of acrylic acid, methacrylic acid, or one of their simple esters crosslinked with an allyl ether of sucrose or an allyl ether of pentaerythritol. Such polymers are commonly sold under the Carbopol or Pemulen trade names, and have the CTFA name Carbomer.

[0113] One particularly suitable type of aqueous phase thickener is an acrylate-based polymer thickener sold by Clariant under the Aristoflex trademark, such as Aristoflex AVC, which is ammonium acryloyldimethyltaurate / VP copolymer; Aristoflex AVL, which is the same polymer found in AVC dispersed in a mixture containing caprylic / capric triglyceride, trilaureth-4, and polyglyceryl-2 sesquiisostearate; or Aristoflex HMB, which is ammonium acryloyldimethyltaurate / beheneth-25 methacrylate crosspolymer, and the like.

[0114] Likewise, suitable as thickening agents are various polyethylene glycol (PEG) derivatives having a degree of polymerization ranging from 1,000 to 200,000. Such ingredients are designated by the designation "PEG" followed by the degree of polymerization (in thousands), e.g., PEG-45M, which refers to a PEG having 45,000 repeating ethylene oxide units. Examples of suitable PEG derivatives include PEG 2M, 5M, 7M, 9M, 14M, 20M, 23M, 25M, 45M, 65M, 90M, 115M, 160M, 180M, and the like.

[0115] Also suitable are polyglycerols, which are repeating glycerol moieties wherein the number of repeating moieties ranges from 15 to 200, preferably from about 20 to 100. Examples of suitable polyglycerols include those having the CTFA designations polyglycerol-20, polyglycerol-40, and the like.

[0116] Surfactants

[0117] The compositions of the present invention may contain one or more surfactants. This is particularly desirable when the composition is in the form of an aqueous gel or emulsion. If present, the surfactant may be in the range of about 0.001-50%, preferably about 0.005-40%, more preferably about 0.01-35% by weight of the total composition. Suitable surfactants may be silicone or organic, nonionic, anionic, amphoteric or zwitterionic. Such surfactants include, but are not limited to, those described herein and are well known in the art.

[0118] Vitamins and Antioxidants

[0119] The composition of the present invention may contain vitamins and / or coenzymes and antioxidants. The composition may include vitamins and / or coenzymes, and it is suggested that the amount thereof is within the range of about 0.001-10%, preferably 0.01-8%, and more preferably 0.05-5% by weight of the total composition. Suitable vitamins include ascorbic acid and its derivatives, such as ascorbyl palmitate, tetrahexyldecyl ascorbate, and B vitamins, such as thiamine, riboflavin, pyridoxine, and coenzymes, such as thiamine pyrophosphate, flavin adenine dinucleotide, folic acid, pyridoxal phosphate, tetrahydrofolate, etc. Vitamin A and its derivatives are also suitable. Examples are vitamin A in the form of retinyl palmitate, retinol, retinoic acid, and beta-carotene. Vitamin E and its derivatives are also suitable, such as vitamin E acetate, nicotinic acid ester, or other esters thereof. In addition, vitamin D and K are suitable.

[0120] Suitable antioxidants are ingredients that help prevent or retard spoilage. Examples of antioxidants suitable for use in the compositions of the present invention are potassium sulfite, sodium bisulfite, sodium isoascorbate, sodium metabisulfite, sodium sulfite, propyl gallate, cysteine ​​hydrochloride, butylated hydroxytoluene, butylated hydroxyanisole, and the like.

[0121] preservative

[0122] The composition may contain 0.001-8%, preferably 0.01-6%, more preferably 0.05-5% by weight of the total composition of a preservative. A variety of preservatives are suitable, including, for example, benzoic acid, benzyl alcohol, benzyl hemiformal, benzyl p-hydroxybenzoate, 5-bromo-5-nitro-1,3-dioxane, 2-bromo-2-nitropropane-1,3-diol, butyl p-hydroxybenzoate, phenoxyethanol, methyl p-hydroxybenzoate, propyl p-hydroxybenzoate, diazolidinyl urea, calcium benzoate, calcium propionate, caprylyl glycol, biguanide derivatives, phenoxyethanol, captan, chlorhexidine diacetate, chlorhexidine digluconate, chlorhexidine dihydrochloride, chloroacetamide, chlorobutanol, p-chloro-m-cresol, chlorophenol, chlorothymol, chloroxylenol, m-cresol, o-cresol, DEMAND hydantoin, DEDM dilaurate hydantoin, dehydroacetic acid, diazolidinyl urea, dibromopropionamidine dioxetate, DMDM ​​hydantoin, and the like. In a preferred embodiment, the composition is free of parabens.

[0123] Granular materials

[0124] The compositions of the present invention may contain particulate material in the form of pigments, inert particles, or mixtures thereof. Such particulate material may be present in an amount of about 0.01-75%, preferably about 0.5-70%, and more preferably about 0.1-65% by weight of the total composition. Where the composition may contain a mixture of pigments and powders, suitable ranges include about 0.01-75% pigment and 0.1-75% powder, the weights being based on the weight of the total composition.

[0125] A.Powder

[0126] The particulate material may be a colored or colorless (e.g., white) non-colored powder. Suitable non-colored powders include bismuth oxychloride, titanate mica, fumed silica, spherical silica, polymethyl methacrylate, micronized Teflon, boron nitride, acrylate copolymers, aluminum silicate, aluminum starch octenyl succinate, bentonite, calcium silicate, cellulose, chalk, corn starch, diatomaceous earth, fuller's earth, glyceryl starch, hectorite, hydrated silicon dioxide, kaolin, magnesium aluminum silicate, magnesium trisilicate, maltodextrin, montmorillonite, microcrystalline cellulose, rice starch, silicon dioxide, talc, mica, titanium dioxide, zinc laurate, zinc myristate, zinc rosinate, alumina, attapulgite, calcium carbonate, calcium silicate, dextran, kaolin, nylon, silica silylate, silk powder, sericite, soy flour, tin oxide, titanium hydroxide, trimagnesium phosphate, walnut shell powder, or mixtures thereof. The above mentioned powders may be surface treated with lecithin, amino acids, mineral oils, silicones or various other agents, alone or in combination, which coat the powder surface and render the particles more lipophilic in nature.

[0127] B. Pigments

[0128] The particulate material may comprise various organic and / or inorganic pigments. Organic pigments are typically various aromatic types, including azo, indigo, triphenylmethane, anthraquinone and xanthine dyes, which are named D&C and FD&C blue, brown, green, orange, red, yellow, etc. Organic pigments are typically composed of insoluble metal salts of certified color additives, known as lakes. Inorganic pigments include iron oxides, ultramarine, chromium, chromium hydroxide colorants and mixtures thereof. Red, blue, yellow, brown, black iron oxides and mixtures thereof are suitable.

[0129] Carrier

[0130] The composition comprises a dermatologically or cosmetically acceptable carrier for skin care active materials. As used herein, "dermatologically / cosmetically acceptable" means that the composition or component is suitable for use in contact with human keratinous tissue without undue toxicity, incompatibility, instability, allergic response, etc. The composition may comprise from about 50% to about 99.99%, or from about 60% to about 99.9%, or from about 70% to about 98%, and or from about 80% to about 95% of the composition.

[0131] The carrier can be of various types, and its non-limiting examples include solutions, dispersions, emulsions and combinations thereof." emulsions" generally contain an aqueous phase and an oil phase. The oil can be derived from an animal, a plant or petroleum, can be natural or synthetic, and can include silicone oils. Emulsion carriers include, but are not limited to, oil-in-water, water-in-oil and water-in-oil-in-water emulsions. In one embodiment, the carrier comprises an oil-in-water emulsion, a water-in-oil emulsion, a water-in-silicone emulsion and / or a water-in-silicone emulsion. The emulsion can include about 0.01% to about 10%, and or about 0.1% to about 5% of a nonionic, anionic or cationic emulsifier and a combination thereof. Suitable emulsifiers are disclosed in, for example, U.S. Patent No. 3,755,560, U.S. Patent No. 4,421,769 and McCutcheon's Detergents and Emulsifiers, North American Edition, pp. 317-324 (1986).

[0132] The present invention will be further described in conjunction with the following examples, which are set forth for purposes of illustration only.

[0133] experiment

[0134] A non-limiting exemplary skin permeation study was conducted to evaluate the effects of the compositions disclosed herein in Example 1 comprising active ingredients of varying hydrophilicity. The formulations used in the experiments are shown in Table 1.

[0135] Example 1

[0136] Table 1: Exemplary Formulations

[0137]

[0138]

[0139] All formulations shown in Table 1 include 1.8% AA2G, 0.1% caffeine and 0.18% hydrophobic active substances. The formulations are prepared by mixing the active ingredients with chopped polymer nanofibers or microfibers. Cellulose acetate microfibers or cellulose acetate nanofibers are used and experiments are conducted according to the present invention. This study evaluates the penetration efficacy of active ingredients in the presence of chopped nanofibers in the composition. The delivery and penetration efficacy of active substances with different hydrophilicities in the formulations were studied. Specifically, the delivery efficacy of caffeine, hydrophobic active substances and ascorbic acid 2-glucoside (AA2G) from formulations containing hydrophobic nanofibers was analyzed. The active ingredients studied cover a range from hydrophilic to very hydrophobic. Two types of polymer nanofibers are used. In order to better disperse the polymer nanofibers, the nanofibers are pre-cut into a length of several centimeters. Such nanofibers are called "chopped fibers".

[0140] The data indicated that in the presence of cellulose acetate chopped fibers, the delivery of AA2G into the skin was enhanced within the first 6 hours after loading. Permeation studies were then performed using a formulation without fibers as a control. The permeation efficacy of caffeine and AA2G was then compared.

[0141] Skin penetration study using MatTek Skin (Franz Cell)

[0142] In a 6-well plate, a MatTek 300 skin (a skin model constructed from living cells, obtained from MatTek) was placed in the wells along with 2.00 mL of incubation medium (also provided by MatTek). 400 μl of sample was loaded onto the top of the skin. The skin was then incubated at 37°C. At 3 hours and 6 hours, the incubation medium was collected and an additional 2.00 mL of medium was added to the wells. The skin was then incubated until 24 hours. Finally, the medium was collected at the 24 hour time point. The collected medium was filtered through a 0.45 μm PTFE injection filter into an HPLC vial. The sample was then subjected to UPLC studies.

[0143] UPLC analysis

[0144] Collected samples from permeation studies (Franz Cell studies) were analyzed using a Waters ACQUITY H UPLC. The UPLC parameters are shown in Table 2.

[0145] Table 2: UPLC study parameters

[0146]

[0147]

[0148] Calibration curves for each active substance were prepared using the standards.

[0149] Skin PAMPA Research

[0150] The in vitro delivery of active ingredients in the formulations was evaluated using a skin PAMPA study. The active ingredients and crushed fine fibers were added to the formulations and permeation tested for polar compounds using a PAMPA SC membrane. The following samples were prepared. The formulations are provided in percentages in Table 3.

[0151] • Formulation 1, such as shown in Table 3.

[0152] • Formulation 2: Formulation 1 and 1% microcrystalline cellulose (9 μM).

[0153] • Formulation 3: Formulation 1 and a maximum amount of 1% dispersion (cellulose acetate fiber / water).

[0154] • Formulation 4: Formulation 1 and 1% microcrystalline cellulose (9 μm) and a maximum amount of 1% dispersion (cellulose acetate fiber / water).

[0155] Table 3 .

[0156]

[0157] Infiltration of AA2G in the presence of chopped nanofibers

[0158] AA2G is a very hydrophilic skin care active. The percentage permeation of AA2G from the formulation obtained from the skin model (Franz Cell) permeation study is shown in Figure 1A and 1B At the 3-hour and 6-hour time points, the formulation containing cellulose acetate chopped fibers showed higher penetration efficacy than the control. The enhancement was 33% at the 3-hour time point, while the enhancement was 30% at the 6-hour time point. At 24 hours, the difference was not significant, less than 10%, indicating that the chopped fibers can enhance the penetration of AA2G for a specific duration (i.e., in the early stages after application).

[0159] Figure 2A and 2B The permeability of AA2G in skin PAMPA studies is shown.

[0160] Figure 2A The results of the skin PAMPA study are shown and indicate that the control without fibers (Formulation 1) did not show the penetration of the active ingredient as obtained in the PAMPA study. However, the cellulose acetate (chopped nanofibers) with the active showed a high penetration of the active followed by Figure 2A Combination of microfibers and nanofibers.

[0161] Figure 2BThe permeability of AA2G in the skin PAMPA study is shown, indicating that the delivery of AA2G in the control and Vivapure (1% microcrystalline cellulose (9 μm)) is very low, while the delivery and permeability of AA2G are significantly increased in the formulations with chopped fibers (Formulation 3: 1% dispersion containing cellulose acetate fibers / water, and Formulation 4: 1% microcrystalline cellulose and dispersion containing 1% cellulose acetate fibers / water).

[0162] Based on skin model penetration ( Figure 1A and 1B ) Franz Cell study, the enhancement was 33% at 3 hours and 30% at 6 hours. At 24 hours, the difference was not so significant (less than 10%). This indicates that the short-cut fibers can enhance the penetration of AA2G within a specific duration (i.e., in the early stage after application). We analyzed the statistical significance of the percentage penetration of AA2G between the control and the formulation containing cellulose acetate fibers. The p value was greater than 0.05. Combined with the PAMPA study results (e.g., in Figure 2A As shown in ), the data from this study showed that formulations with cellulose acetate short-cut fibers enhanced the permeability of AA2G. Figure 2B The penetration shown is about 0-300 μg / cm2.

[0163] Figure 2C Results from a skin model permeation (Franz Cell) study at the 3 hour time point are shown. Figure 2D Shown are the comparative results of the 6-hour permeation of AA2G between the skin PAMPA study and the skin permeation study results.

[0164] Example 2

[0165] Permeation of caffeine in the presence of chopped nanofibers

[0166] Caffeine is an active ingredient that is both water-soluble and oil-soluble. Figure 3 A study showing the penetration of caffeine into a chopped fiber formulation over a 24-hour period. Figure 3 As shown in the data, there was no significant enhancement of caffeine penetration (<10%) by the addition of chopped fibers at the 6 hour time point.

[0167] The results of a skin PAMPA study of caffeine are shown in Figure 4A and 4B middle. Figure 4A The results of the PAMPA study showed that Figure 4B The delivery of caffeine in the composition comprising chopped fibers was shown to result in double the penetration in the presence of chopped fibers compared to the control. Figure 4B is the result of skin PAMPA research.

[0168] Figure 4A Data from a 6-hour permeation study of caffeine obtained through skin PAMPA showed that cellulose acetate nanofibers slightly increased the amount of active ingredient permeated, followed by a combination of microfibers and nanofibers. This result is consistent with the results of the permeation study of AA2G.

[0169] Figure 4C Results showing skin model penetration (FranzCell) of the delivery of caffeine from a formulation with added chopped fibers.

[0170] Figure 3 The results at the 6 hour time point can be explained by the data obtained from the skin PAMPA study. Since an artificial skin layer was used in the skin PAMPA study, there may be studies that mimic living skin layers through polymers and variants. Additionally, the % penetration of caffeine was greater than the % penetration of AA2G, which is consistent with previous permeation studies using other crèmes and computer modeling. [[Related to the benefits of the claim]]

[0171] Example 3

[0172] Penetration of hydrophobic active substances in the presence of chopped nanofibers

[0173] A hydrophobic active substance was used in this study. It is insoluble in water. Figure 5 Shows the results of the penetration of hydrophobic active substances and short-cut fibers through a skin penetration study (FranzCell) over 24 hours. Figure 5 The data shown in , show that the penetration of hydrophobic actives is not significantly enhanced by the addition of chopped fibers. Figure 6 This is consistent with the data obtained from the skin PAMPA studies shown in .

[0174] The applicants of the present invention unexpectedly and surprisingly achieved cosmetic benefits of improving the appearance of the skin using the composition. The composition provides significant benefits, showing reduction of venous dilation, eye bags, dark circles and swelling around the eyes, fine lines and wrinkles, loss of elasticity, wrinkles, loss of hardness, loss of uniformity of color or tone, rough surface texture, age spots, and reduction of moisture content in the skin around the eyes and facial skin. Additional benefits obtained by the composition include, but are not limited to, using the composition as an antioxidant, collagen promoter, and to lighten dark spots, smooth wrinkles, promote healing and / or reduce scars.

[0175] Experiments and analysis of results indicate that in the presence of chopped fibers, the penetration efficacy of multiple actives is enhanced by both skin penetration studies and PAMPA studies. Modeled skin consists of living cells, and they are used to better simulate the permeability of the skin. Cellulose acetate chopped fibers enhance the penetration of AA2G and slightly enhance the penetration of caffeine. There is a hydrophobic dependence in the enhancement. Cellulose acetate microfibers (dispersed) do not show significant enhancement in the penetration of all selected active substances. However, dispersed / chopped nanofibers show enhanced penetration of actives. Such results are consistent with the results obtained by skin PAMPA studies.

[0176] Unless expressly excluded or otherwise limited, each document cited herein (including any cross-referenced or related patents or applications) is incorporated herein by reference in its entirety. The citation of any document is not an admission that it is prior art to any invention disclosed or claimed herein, or that it teaches, suggests or discloses any such invention, either alone or in any combination with any other reference or references. In addition, when any meaning or definition of a term in this document conflicts with any meaning or definition of the same term in a document incorporated by reference, the meaning or definition assigned to the term in this document shall prevail.

[0177] Although specific embodiments of the present invention have been illustrated and described, it will be apparent to those skilled in the art that many other changes and modifications may be made without departing from the scope of the invention. Therefore, the appended claims are intended to cover all such changes and modifications within the scope of the invention.

Claims

1. A cosmetic composition comprising electrospun polymer hydrophobic nanofibers, at least one active ingredient and at least one cosmetically acceptable carrier, wherein the at least one active ingredient comprises ascorbic acid 2-glucoside or caffeine, wherein the electrospun polymer hydrophobic nanofibers comprise cellulose acetate fibers and have a diameter of 0.5 nm to 0.5 µm, wherein the electrospun polymer hydrophobic nanofibers are dispersed in the composition, wherein the composition is in the form of an emulsion, cream, lotion, gel, slurry, suspension, paste or foam.

2. The composition of claim 1, wherein the at least one active ingredient further comprises one or more of peptides, DNA, RNA, proteins, vitamins, organic acids and oils.

3. The composition of claim 2, wherein the at least one active ingredient comprises an enzyme.

4. The composition of claim 1, wherein the electrospun polymeric hydrophobic nanofibers are present in the composition at 0.01 wt% to 10 wt% relative to the total weight of the composition.

5. The composition of claim 1, wherein the composition further comprises an antioxidant, a moisturizer, a surfactant or a wetting agent.

6. A non-therapeutic method for reducing skin imperfections on a subject's skin, the method comprising topically applying a cosmetic composition comprising electrospun polymeric hydrophobic nanofibers and at least one active ingredient, the at least one active ingredient comprising ascorbic acid 2-glucoside or caffeine, wherein the electrospun polymeric hydrophobic nanofibers comprise cellulose acetate fibers and have a diameter of 0.5 nm to 0.5 µm, wherein the electrospun polymeric hydrophobic nanofibers are dispersed in the composition, wherein the composition is in the form of an emulsion, cream, lotion, gel, slurry, suspension, paste, or foam.

7. The non-therapeutic method of claim 6, wherein the composition further comprises one or more cosmetically acceptable carriers.

8. The non-therapeutic method of claim 6, wherein the method further improves the visual appearance of the subject's skin.

9. The non-therapeutic method of claim 6, wherein the method further improves venous dilation, eye bags, dark circles, swelling around the eyes, fine lines, wrinkles, loss of elasticity, loss of firmness, loss of color uniformity, tone, rough surface or texture, age spots, or moisture content in the skin.

10. The non-therapeutic method according to claim 6, wherein the composition is administered at least once a day.

11. The non-therapeutic method according to claim 6, wherein the composition is administered in the morning and at night before going to bed.

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