Gene Sequencing Device

Abstract

The present invention provides a gene sequencing device, which includes a pre-filling mechanism, a reagent drive unit, and a flow cell. The pre-filling mechanism includes a base and a first pre-filling unit. The base has a first flow channel, and the first pre-filling unit has a second flow channel. When the first pre-filling unit is in a first pre-filling position, the first flow channel and the second flow channel are connected. When the first pre-filling unit is in a first storage position, the first flow channel and the second flow channel are disconnected. The reagent drive unit is used to drive the flow of reagent, and the reagent drive unit is connected to the first flow channel. The flow cell has a third flow channel. When the flow cell is in a connected position and the first pre-filling unit is in a first storage position, the third flow channel and the first flow channel are connected. Before testing the flow cell, this solution moves the first pre-filling unit to the first pre-filling position and operates the reagent drive unit. This expels bubbles in the first flow channel, thereby preventing the quality of the sample to be tested from being affected by the bubbles and improving the detection accuracy.

Description

Technical Field

[0001] The present invention relates to the technical field of detection devices, and in particular to a gene sequencing device. Background Art

[0002] In existing genetic testing instruments, samples are fixed in a flow cell (i.e., a chip), and the flow cell and detection reagents are fluidically connected through pipelines. Before starting to test the sample, if the air in the pipeline is not completely emptied, it will affect the instrument's pumping accuracy, reaction completeness, and detection accuracy.

[0003] To ensure instrument performance, before the various reagents in the reagent tank pass through the detection flow cell, the air in the instrument pipelines must be evacuated and filled with the corresponding reagents. This step is generally called pre-priming. Recently developed bypass-assisted replacement technology can be used to pre-prime reagents before the flow cell, but this solution still retains bubbles in the main flow path and can be pumped into the flow cell, affecting detection accuracy. Summary of the Invention

[0004] The present invention provides a gene sequencing device to eliminate or reduce air residue in a pipeline after pre-infusion of reagents in the gene sequencing device, thereby improving detection accuracy.

[0005] In order to achieve the above-mentioned objectives, the present invention provides a gene sequencing device, comprising: a pre-filling mechanism, the pre-filling mechanism comprising a base and a first pre-filling part, the base having a first flow channel, the first pre-filling part having a second flow channel, the first pre-filling part being movable to a first pre-filling position or a first storage position, wherein when the first pre-filling part is in the first pre-filling position, the first flow channel and the second flow channel are connected, and when the first pre-filling part is in the first storage position, the first flow channel and the second flow channel are disconnected; a reagent driving part, used to drive the flow of reagent, the reagent driving part and the first flow channel being connected; a flow cell, the flow cell having a third flow channel, the flow cell being movable to a connection position, when the flow cell is in the connection position, the first pre-filling part is in the first storage position, and the third flow channel and the first flow channel are connected.

[0006] Furthermore, the flow pool can be moved to a separation position. When the flow pool is in the separation position, the third flow channel and the first flow channel are disconnected, and the first pre-filling part is in the first pre-filling position.

[0007] Furthermore, the pre-filling mechanism also includes: a rebound mechanism, which applies elastic force to the first pre-filling part, and when the flow pool moves to the connection position, the flow pool pushes the first pre-filling part to move to the first storage position; when the flow pool leaves the connection position, the rebound mechanism pushes the first pre-filling part to move to the first pre-filling position.

[0008] Furthermore, the pre-filling mechanism also includes: a fixed seat, the fixed seat is connected to the base, the fixed seat has a guide hole, the first pre-filling part can be slidably inserted into the guide hole, and the two ends of the rebound mechanism are respectively in contact with the fixed seat and the first pre-filling part.

[0009] Furthermore, the first pre-filling portion is hinged to the base, the rebound mechanism is a torsion spring provided on the base, and the rebound mechanism abuts against the first pre-filling portion.

[0010] Furthermore, the pre-filling mechanism further includes: a sealing mechanism, wherein when the first pre-filling portion is in the first pre-filling position, the sealing mechanism applies a force on the first pre-filling portion toward the base.

[0011] Furthermore, the sealing mechanism includes: a first magnetic member, which is arranged on the base; a second magnetic member, which is arranged on the first pre-filling part. When the first pre-filling part is in the first pre-filling position, the first magnetic member and the second magnetic member are attracted to each other.

[0012] Furthermore, the sealing mechanism includes: a connecting member, which is arranged on the base; and an elastic pressing piece, which is connected to the connecting member. When the first pre-filling part is in the first pre-filling position, the elastic pressing piece presses the first pre-filling part.

[0013] Furthermore, the pre-filling mechanism also includes: a first sealing ring, which is arranged around the opening of the second flow channel; a second sealing ring, which is arranged around the opening of the third flow channel; wherein, when the first pre-filling part is in the first pre-filling position, the first sealing ring and the opening of the first flow channel are connected; when the flow pool is in the connection position, the second sealing ring and the opening of the first flow channel are connected.

[0014] Furthermore, the base has multiple first flow channels, the second flow channels have multiple openings, and the flow pool has multiple third flow channels; wherein, when the first pre-filling part is in the first pre-filling position, the multiple openings of the multiple first flow channels and the second flow channels are connected one-to-one; when the flow pool is in the connection position, the multiple first flow channels and the multiple third flow channels are connected one-to-one.

[0015] Furthermore, the base also has a fourth flow channel, which is spaced apart from the first flow channel, and the pre-filling mechanism also includes a second pre-filling part, the second pre-filling part has a fifth flow channel, and the fifth flow channel is connected to the second flow channel; the second pre-filling part can be moved to a second pre-filling position or a second storage position, wherein, when the second pre-filling part is in the second pre-filling position, the fourth flow channel and the fifth flow channel are connected, and when the second pre-filling part is in the second storage position, the fourth flow channel and the fifth flow channel are disconnected; when the flow pool is in the connection position, the second pre-filling part is in the second storage position, and the two ends of the third flow channel are respectively connected to the first flow channel and the fourth flow channel.

[0016] Furthermore, the flow pool can also be moved to a separation position. When the flow pool is in the separation position, the third flow channel and the first flow channel and the fourth flow channel are all disconnected, the first pre-filling part is in the first pre-filling position, and the second pre-filling part is in the second pre-filling position; the pre-filling mechanism also includes a hose, and the fifth flow channel and the second flow channel are connected by the hose; in the process of moving the flow pool to the connection position, the flow pool pushes the first pre-filling part to move to the first storage position, and the flow pool pushes the second pre-filling part to move to the second storage position.

[0017] Furthermore, the gene sequencing device also includes: a reagent storage module, which is used to store different types of reagents; a reagent switching module, to which the reagent storage module and the base are connected, and the reagent switching module is used to select different types of reagents to be supplied to the first flow channel of the base; and a waste liquid collection module, which is used to collect waste liquid output from the pre-infusion mechanism and the flow pool.

[0018] The technical solution of the present invention provides a gene sequencing device, comprising a pre-filling mechanism, a reagent drive unit, and a flow cell. The pre-filling mechanism comprises a base and a first pre-filling unit, the base having a first flow channel, the first pre-filling unit having a second flow channel, wherein when the first pre-filling unit is in a first pre-filling position, the first flow channel and the second flow channel are connected, and when the first pre-filling unit is in a first storage position, the first flow channel and the second flow channel are disconnected. The reagent drive unit is used to drive the flow of reagent, and the reagent drive unit is connected to the first flow channel. The flow cell has a third flow channel, and when the flow cell is in a connection position and the first pre-filling unit is in a first storage position, the third flow channel and the first flow channel are connected. Using this solution, before testing the flow cell, the first pre-filling unit is moved to the first pre-filling position and the reagent drive unit is operated. This forms a flow path, allowing sufficient reagent to flow through the first flow channel, thereby discharging bubbles in the first flow channel through the second flow channel, eliminating or reducing bubbles in the first flow channel. The flow cell is then connected to the first flow channel for testing, thereby preventing the quality of the sample to be tested from being affected by bubbles and improving testing accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The accompanying drawings, which constitute part of this application, are intended to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are intended to explain the present invention and do not constitute an undue limitation of the present invention. In the accompanying drawings:

[0020] Figure 1 1 shows a schematic structural diagram of a gene sequencing device provided in Embodiment 1 of the present invention (the first pre-filling unit is in the first storage position);

[0021] Figure 2 Shown Figure 1 Another structural schematic diagram of the gene sequencing device in (the first pre-filling part is in the first pre-filling position);

[0022] Figure 3 Shown Figure 1 sectional view of

[0023] Figure 4 The figure shows the liquid connection diagram of the gene sequencing device in Example 1 when no flow cell is inserted;

[0024] Figure 5 The figure shows the fluid connection diagram of the gene sequencing device in Example 1 when a flow cell is inserted;

[0025] Figure 6 1 shows a schematic structural diagram of a gene sequencing device provided in a second embodiment of the present invention (the first pre-filling unit is in the first storage position);

[0026] Figure 7 Shown Figure 6 Another structural schematic diagram of the gene sequencing device in (the first pre-filling part is in the first pre-filling position);

[0027] Figure 8 Shown Figure 6 sectional view of

[0028] Figure 9 1 shows a schematic structural diagram of a gene sequencing device provided in a third embodiment of the present invention (the first pre-filling unit is in the first storage position);

[0029] Figure 10 Shown Figure 9 Another structural schematic diagram of the gene sequencing device in (the first pre-filling part is in the first pre-filling position);

[0030] Figure 11 Shown Figure 9 sectional view of

[0031] Figure 12 1 shows a schematic structural diagram of a gene sequencing device provided in a fourth embodiment of the present invention (the first pre-filling unit is in the first storage position);

[0032] Figure 13 Shown Figure 12 Another structural schematic diagram of the gene sequencing device in (the first pre-filling part is in the first pre-filling position);

[0033] Figure 14 The figure shows the fluid connection diagram of the gene sequencing device in Example 4 when no flow cell is inserted;

[0034] Figure 15 The figure shows the fluid connection diagram of the gene sequencing device in Example 4 when a flow cell is inserted;

[0035] Figure 16A schematic diagram of the process of performing reagent pre-infusion in an embodiment of the present invention is shown.

[0036] The above drawings include the following reference numerals:

[0037] 11. Base; 12. First flow channel; 13. Fixed seat; 15. First sealing ring; 16. Second sealing ring; 17. Fourth flow channel; 18. Hose; 20. First pre-filling part; 21. Second flow channel; 31. Reagent drive part; 32. Reagent storage module; 33. Reagent switching module; 34. Waste liquid collection module; 40. Flow pool; 41. Third flow channel; 50. Rebound mechanism; 60. Sealing mechanism; 61. First magnetic element; 62. Second magnetic element; 63. Connecting part; 64. Elastic pressing piece; 70. Second pre-filling part; 71. Fifth flow channel. DETAILED DESCRIPTION

[0038] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. The following description of at least one exemplary embodiment is actually only illustrative and is in no way intended to limit the present invention and its application or use. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative work are within the scope of protection of the present invention.

[0039] like Figures 1 to 5 As shown, an embodiment of the present invention provides a gene sequencing device, comprising: a pre-filling mechanism, comprising a base 11 and a first pre-filling unit 20. The base 11 has a first flow channel 12, and the first pre-filling unit 20 has a second flow channel 21. The first pre-filling unit 20 is movable to a first pre-filling position or a first storage position. When the first pre-filling unit 20 is in the first pre-filling position, the first flow channel 12 and the second flow channel 21 are connected. When the first pre-filling unit 20 is in the first storage position, the first flow channel 12 and the second flow channel 21 are disconnected. A reagent driving unit 31 is used to drive the flow of reagents. The reagent driving unit 31 is connected to the first flow channel 12. A flow cell 40 has a third flow channel 41. The flow cell 40 is movable to a connection position. When the flow cell 40 is in the connection position, the first pre-filling unit 20 is in the first storage position, and the third flow channel 41 is connected to the first flow channel 12. The disconnection of two flow channels means that the two flow channels are not connected. The flow cell 40 can also be understood as a chip. The base 11 can also be referred to as a fluid manifold block.

[0040] The connection between the reagent driving unit 31 and the first flow channel 12 is not limited to direct connection between the two, and can also be connected through other pipelines. Moreover, the reagent driving unit 31 can be located upstream or downstream of the first flow channel 12, as long as it can drive the flow of the reagent in the first flow channel 12.

[0041] With this solution, before testing the flow pool 40, the first pre-filling part 20 is first moved to the first pre-filling position and the reagent driving part 31 is operated, so that the first flow channel 12 and the second flow channel 21 are connected, and the reagent can flow, forming a flow path. Sufficient reagent flows through the first flow channel 12, so that the bubbles in the first flow channel 12 can be discharged from the second flow channel 21, eliminating or reducing the bubbles in the first flow channel 12. Then, the flow pool 40 is connected to the first flow channel 12, so that the reagent enters the flow pool 40 for testing, thereby avoiding the quality of the sample to be tested being affected by bubbles and improving the detection accuracy.

[0042] In this embodiment, the flow cell 40 can also be moved to a separate position. When the flow cell 40 is in the separate position, the third flow channel 41 and the first flow channel 12 are disconnected, and the first pre-filling unit 20 is in the first pre-filling position. That is, after a flow cell 40 test is completed, the flow cell 40 is removed from the connection position, and the first pre-filling unit 20 is moved back to the first pre-filling position. This connects the first flow channel 12 and the second flow channel 21, ensuring that the first flow channel 12 remains filled with reagent, thereby preventing air bubbles from entering and ensuring the quality of the next test.

[0043] In this embodiment, the pre-filling mechanism further includes a rebound mechanism 50, which applies an elastic force to the first pre-filling portion 20. When the flow cell 40 moves to the connected position, the flow cell 40 pushes the first pre-filling portion 20 to the first storage position. When the flow cell 40 leaves the connected position, the rebound mechanism 50 pushes the first pre-filling portion 20 to the first pre-filling position. With this solution, when the flow cell 40 leaves the connected position, the elastic force of the rebound mechanism 50 moves the first pre-filling portion 20 to the first pre-filling position, eliminating the need for manual operation and improving the user experience.

[0044] In this embodiment, the pre-filling mechanism further includes a fixed seat 13 connected to the base 11. The fixed seat 13 has a guide hole, through which the first pre-filling portion 20 is slidably disposed. The two ends of the rebound mechanism 50 abut against the fixed seat 13 and the first pre-filling portion 20, respectively. The guide hole guides the first pre-filling portion 20 so that it moves back and forth along a predetermined path. Specifically, the rebound mechanism 50 can be a spring or other structure.

[0045] In this embodiment, the pre-filling mechanism further includes a sealing mechanism 60. When the first pre-filling portion 20 is in the first pre-filling position, the sealing mechanism 60 applies a force to the first pre-filling portion 20 toward the base 11. This ensures that the first flow channel 12 and the second flow channel 21 maintain a sealed connection through the sealing mechanism 60, preventing leakage of the reagent.

[0046] Specifically, the sealing mechanism 60 includes a first magnetic member 61 disposed on the base 11 and a second magnetic member 62 disposed on the first pre-filling portion 20. When the first pre-filling portion 20 is in the first pre-filling position, the first magnetic member 61 and the second magnetic member 62 engage. This means that sealing is achieved through the mutual attraction between the first and second magnetic members 61, 62. The first pre-filling portion 20 is T-shaped, and there are two second magnetic members 62. The opening of the second flow channel 21 is located between the two second magnetic members 62, which improves the sealing effect.

[0047] Or, as Figures 6 to 8 As shown, in the second embodiment of the present invention, unlike the above embodiments, the sealing mechanism 60 includes: a connecting member 63, which is disposed on the base 11; and an elastic pressing piece 64, which is connected to the connecting member 63. When the first pre-filling portion 20 is in the first pre-filling position, the elastic pressing piece 64 presses the first pre-filling portion 20. The elastic pressing piece 64 is elastic. When the first pre-filling portion 20 is in the first pre-filling position, the elastic pressing piece 64 presses the first pre-filling portion 20, thereby maintaining the seal.

[0048] In either Example 1 or Example 2, the pre-infusion mechanism further includes: a first sealing ring 15 disposed around the opening of the second flow channel 21; and a second sealing ring 16 disposed around the opening of the third flow channel 41. When the first pre-infusion unit 20 is in the first pre-infusion position, the first sealing ring 15 abuts against the opening of the first flow channel 12; and when the flow cell 40 is in the connection position, the second sealing ring 16 abuts against the opening of the first flow channel 12. The provision of the sealing rings improves the sealing effect and prevents leakage of reagents at the connection between the two flow channels.

[0049] like Figures 9 to 11 As shown, in the third embodiment, unlike the above embodiments, the base 11 has multiple first flow channels 12, the second flow channel 21 has multiple openings, and the flow cell 40 has multiple third flow channels 41. When the first pre-filling portion 20 is in the first pre-filling position, the multiple first flow channels 12 and the multiple openings of the second flow channels 21 are connected in a one-to-one correspondence; when the flow cell 40 is in the connected position, the multiple first flow channels 12 and the multiple third flow channels 41 are connected in a one-to-one correspondence. This allows samples in multiple flow channels of the flow cell 40 to be tested simultaneously, improving detection efficiency.

[0050] like Figures 12 to 15As shown, in the fourth embodiment of the present invention, unlike the above embodiments, the first pre-filling portion 20 is hinged to the base 11, and the rebound mechanism 50 is a torsion spring provided on the base 11. The rebound mechanism 50 abuts against the first pre-filling portion 20. The rebound mechanism 50 can drive the first pre-filling portion 20 to rotate.

[0051] Furthermore, the base 11 also has a fourth flow channel 17, and the fourth flow channel 17 and the first flow channel 12 are arranged at intervals. The pre-filling mechanism also includes a second pre-filling part 70, and the second pre-filling part 70 has a fifth flow channel 71, and the fifth flow channel 71 is connected to the second flow channel 21; the second pre-filling part 70 can be moved to a second pre-filling position or a second storage position, wherein, when the second pre-filling part 70 is in the second pre-filling position, the fourth flow channel 17 and the fifth flow channel 71 are connected, and when the second pre-filling part 70 is in the second storage position, the fourth flow channel 17 and the fifth flow channel 71 are disconnected; when the flow pool 40 is in the connection position, the second pre-filling part 70 is in the second storage position, and the two ends of the third flow channel 41 are respectively connected to the first flow channel 12 and the fourth flow channel 17. That is, when the second pre-filling unit 70 is in the second storage position and the first pre-filling unit 20 is in the first pre-filling position, the first flow channel 12, the second flow channel 21, the fifth flow channel 71, and the fourth flow channel 17 are connected, thus forming a flow path, thereby allowing for exhaust and preventing bubbles in the flow channel from affecting the quality of detection. The second pre-filling unit 70 can be configured with the same structure as the first pre-filling unit 20.

[0052] In this embodiment, the flow cell 40 can also be moved to a separated position. When the flow cell 40 is in the separated position, the third flow channel 41 is disconnected from the first flow channel 12 and the fourth flow channel 17. The first pre-filling part 20 is in the first pre-filling position, and the second pre-filling part 70 is in the second pre-filling position. The pre-filling mechanism also includes a hose 18, and the fifth flow channel 71 and the second flow channel 21 are connected by the hose 18. When the flow cell 40 moves to the connected position, the flow cell 40 pushes the first pre-filling part 20 to the first storage position, and the flow cell 40 pushes the second pre-filling part 70 to the second storage position. That is, when the flow cell 40 moves to the connected position, the first flow channel 12, the third flow channel 41, and the fourth flow channel 17 are connected, so that the flow cell 40 can be tested.

[0053] In the above embodiment, the gene sequencing apparatus further includes: a reagent storage module 32 for storing different types of reagents; a reagent switching module 33 connected to both the reagent storage module 32 and the base 11, for selecting different types of reagents to supply to the first flow channel 12 of the base 11; and a waste liquid collection module 34 for collecting waste liquid output from the pre-filling mechanism and the flow cell 40. Optionally, the gene sequencing apparatus further includes a sensor for detecting the position of the first pre-filling section 20 to determine whether the first pre-filling section 20 has been moved into position.

[0054] The existing pre-infusion method requires manual insertion of a used flow cell to connect the flow cell inlet and outlet, allowing for pre-infusion of reagents before the flow cell inlet. This process requires manual replacement of the used flow cell with the test flow cell after infusion. This solution currently suffers from issues such as a low degree of automation and a poor user experience. To better understand this solution, further explanation is provided below.

[0055] The pre-filling device (i.e., the first pre-filling unit or the second pre-filling unit) consists of three parts, including 1) a sealing mechanism, 2) a rebound mechanism, and 3) a fluid pipeline structure. Its structural and material characteristics are as follows:

[0056] 1) The sealing mechanism includes a sealing gasket, a sealing gasket fixing head, and a sealing fastening device.

[0057] The sealing gasket is a component with a through hole, the diameter of which is greater than or equal to the diameter of the fluid pipeline, and the arrangement includes single-hole, multi-hole, or multi-hole and series-parallel forms, and the material is engineering plastic, metal, rubber ring, rubber, polydimethylsiloxane (PDMS) and other elastomers, as well as composite materials or combinations of the above materials;

[0058] The gasket fixing head is used to secure the gasket. It contains a flow channel, one end of which connects to one or more through-holes in the gasket and the other end to the pre-injection pipeline at the rear end. This mechanism also has a structure that is fixed to the rebound mechanism to achieve force or torque transmission between the two. This component is made of engineering plastics (such as PP, PEI, PEEK, etc.) or metal.

[0059] The sealing fastening device is used to apply pressure to the sealing ring in the direction of the fluid manifold block to ensure sealing reliability. In different embodiments, it can be a retaining spring, a spring, a magnet, an electromagnet and other components (not limited to the above components).

[0060] The sealing mechanism is movable relative to the fluid manifold block (i.e., the base). It has two operating positions: 1. Pre-priming position, which is when the sealing mechanism is fluidically connected to the fluid manifold block. 2. Storage position, which is when the flow cell is inserted and the sealing mechanism is pushed out of the flow cell and comes to rest.

[0061] 2) The rebound mechanism includes an elastomer and a mechanism mount. The mechanism mount is used to support and secure one or more compression mechanisms, rebound mechanisms, and pre-injected pipes. Materials include, but are not limited to, plastic (PEI, PEEK, PP) and metal.

[0062] The elastomer provides a restoring force or torque when an external force or torque causes relative displacement between the sealing mechanism and the fluid manifold block inlet. This ensures that the sealing mechanism returns to its predetermined position upon removal of the external force or torque, establishing fluid connection with the reagent wells on the fluid manifold block. In specific embodiments, the elastomer can be a spring, torsion spring, elastic rubber pad, magnet, or electromagnet, and can be made of metal, engineering plastic, rubber, or composites or combinations of these materials.

[0063] 3) The fluid piping structure includes pre-filled piping and fluid manifold blocks.

[0064] The pre-infusion pipeline is used to achieve fluid connection between the sealing fixed head and the fluid components such as the pump and valve at the rear end of the machine. Its materials include but are not limited to plastics (PEI, PEEK, PTFE, PFA), metals, etc. The fluid manifold block is used to achieve fluid connection between the various reagent pipelines in the instrument and the sealing mechanism, which may include one or more pipes, sealing gaskets, pumps, valves, reagent needles, flow pool chip fixing seats and other components. The outlet end of the manifold block is an opening for fluid connection with one or more inlets of one or more flow pool chips. At the same time, a sealing gasket can be installed at the opening to ensure that an effective fluid seal is formed with the chip inlet, but the sealing gasket can also be installed at the chip inlet and outlet, thereby reducing the overall pipeline volume. This outlet end can be fluidically connected to the pipeline hole on the sealing gasket fixing head or the flow pool outlet and inlet.

[0065] At the same time, the manifold block also has a clamping mechanism that cooperates with the sealing and fastening device, and its function is: on the one hand, when the sealing gasket fixing head is close to the corresponding reagent outlet on the manifold block, a guiding force or torque is applied to the sealing gasket fixing head to guide the sealing gasket fixing head to be aligned and concentric with the corresponding reagent outlet on the manifold block; on the other hand, when the sealing gasket fixing head is aligned with the manifold block reagent outlet, it can cooperate with the sealing and fastening device to form a positive pressure applied to the sealing ring to ensure that the sealing gasket fixing head and the manifold block reagent outlet form a sealed fluid connection. In a specific embodiment, it can be a magnet, an electromagnet, a card seat, a buckle, a mechanical gripper, and its material can be metal, engineering plastic, rubber, and its material includes but is not limited to plastic (PEI, PEEK, PTFE, PFA (perfluoroalkoxy alkane), metal, etc.

[0066] like Figure 16 As shown, the use process of the pre-filling device mainly includes 6 steps:

[0067] 1) Insert the reagent tank to be pre-filled, and the reagent tank is fluidically connected to the fluid manifold block through pipes, manifold blocks, reagent needles, etc. At this time, there is no need to insert the flow cell chip to be tested.

[0068] 2) When there is no external force or external torque, the sealing gasket fixing head moves to the vicinity of the manifold block outlet end due to the restoring force or restoring torque applied by the elastic body. At this time, the clamping mechanism on the fluid manifold block applies a guiding force or torque to the sealing gasket fixing head through limiting, magnetic attraction, etc., guiding the sealing gasket fixing head to be aligned and concentric with the corresponding reagent outlet on the manifold block; then the clamping mechanism can cooperate with the sealing fastening device through limiting, magnetic attraction, etc. to form a positive pressure applied to the sealing ring, ensuring that the sealing gasket fixing head and the manifold block reagent outlet form a sealed fluid connection.

[0069] 3) When the in-place sensor detects that the sealing gasket fixing head is in the pre-infusion position, the instrument controls the valve in the fluid pipeline structure to form a fluid connection between the reagent to be infused, the pre-infusion pipeline, and the fluid power source (pump). The fluid power source then drives the reagent to be infused through the pipeline, the sealing gasket, the sealing gasket fixing head, and the pre-infusion pipeline at the rear end of the fixing head, thereby realizing the pre-infusion of this reagent in the pipeline before the inlet of the flow pool chip.

[0070] 4) Switch the valve to select the next reagent, so that step 3 is repeated in sequence for different reagents to be perfused until all the predetermined perfusion reagents have completed the perfusion work.

[0071] 5) The chip to be tested is inserted from the side of the prime mechanism by a manipulator or manually. As the chip to be tested is gradually inserted, the chip or chip frame forms a physical interference with the sealing gasket fixing head. The sealing gasket fixing head is pressurized by the external force of the chip and the chip frame, causing the elastic body to deform, thereby causing a relative displacement between the sealing gasket fixing head and the inlet of the fluid manifold block, and the original fluid connection between the two is immediately disconnected. When the chip to be tested continues to be inserted until it is in place, the inlet of the chip to be tested is aligned with the reagent outlet on the fluid manifold block and a fluid connection is achieved, while the sealing gasket fixing head retreats due to the deformation of the elastic body. The instrument then begins to perform related tasks such as pumping liquid, temperature control, and testing on the chip to be tested.

[0072] 6) After the chip is tested, the tested chip is taken out. During the removal process, the external force and external torque on the sealing gasket fixing head gradually disappear, so that the deformation of the squeezed elastomer gradually recovers, and the elastomer pushes the sealing gasket fixing head to move near the outlet end of the manifold block. At this time, the clamping mechanism on the fluid manifold block applies a guiding force or torque to the sealing gasket fixing head through limiting, magnetic attraction, etc., guiding the sealing gasket fixing head to be aligned and concentric with the corresponding reagent outlet on the manifold block; at the same time, the clamping mechanism can cooperate with the sealing fastening device through limiting, magnetic attraction, etc. to form a positive pressure applied to the sealing ring, ensuring that the sealing gasket fixing head and the reagent outlet of the manifold block form a sealed fluid connection.

[0073] Example 1:

[0074] 1. Device structure:

[0075] The fluid manifold block adapted for a single-channel sequencing chip has an inlet flow channel (ie, the first flow channel 12 ).

[0076] The sealing gasket fixing head (i.e., the first pre-filling part 20) is a T-shaped PEI part, one end of which has a flow channel inlet (i.e., the second flow channel 21) that matches the manifold block inlet, and a rubber sealing gasket is installed at the flow channel inlet. A cylindrical neodymium iron boron magnet is installed in the pin holes on both sides of the sealing gasket as a sealing and fastening device, where the polarity of the lower surface of the magnet is opposite to the polarity of the upper surface of the magnet to ensure that the two magnets can attract each other. There is an interface at the end of the fixing head for fluid connection with the pre-filling pipeline, and there is a flow channel inside the fixing head for realizing fluid connection between the fixing head inlet sealing gasket and the outlet connector. At the rear end of the fixing head, there is a stepped reducer for achieving fixation with one end of the rebound mechanism.

[0077] The mechanism fixing seat is a stainless steel pressure block with two screw holes for fixing the fixing seat to the fluid manifold block. There is a stepped through hole in the middle of the fixing block for passing the sealing gasket fixing head 3. At the same time, the step in the through hole is also used to fix the other end of the elastomer.

[0078] A single-channel flow cell sequencing biochip consists of a silicon chip with a chemically modified inner wall on one side and quartz glass on the other. The silicon chip is surrounded by a chip frame, which has sealing gaskets embedded near the chip's inlet and outlet ports.

[0079] The layout of the device is as follows Figure 4 and Figure 5 As shown, the reagent storage module is connected to the reagent switching module, the reagent switching module is connected to the inlet of the fluid manifold block, the pre-infusion structure is connected to the reagent drive unit, and the discharge outlet of the reagent drive unit is connected to the waste liquid collection module. At the same time, the outlet of the fluid manifold block is connected to another inlet of the reagent drive module.

[0080] 2. Reagent pre-infusion:

[0081] 1) Insert the reagent tank to be pre-filled, and the reagent tank is fluidically connected to the fluid manifold block through pipes, manifold blocks, reagent needles, etc. There is no need to insert the chip to be tested at this time.

[0082] 2) In the absence of external force, the spring-loaded seal retainer moves to the vicinity of the manifold block outlet. The magnetic clamping mechanism on the fluid manifold block then magnetically attracts the magnetic seal fastening mechanism on the seal retainer, guiding the seal retainer to align and concentrically align with the corresponding reagent outlet on the manifold block. The attractive force between the two magnetic mechanisms then creates positive pressure on the seal ring, ensuring a sealed fluid connection between the seal retainer and the reagent outlet on the manifold block.

[0083] 3) When the sealing gasket fixing head is in the pre-infusion position, the reagent storage module, the switching module, the fluid manifold block inlet, the pre-infusion mechanism, the reagent driving unit, and the waste liquid collection module form a fluid connection, and then the reagent driving unit works to drive the reagent to be infused through the pipeline and the sealing gasket into the sealing gasket fixing head and then into the pre-infusion pipeline at the rear end of the fixing head, thereby realizing the pre-infusion of this reagent in the pipeline in front of the fluid manifold block inlet.

[0084] 4) The reagent switching module works so that different reagents to be perfused repeat step 3 in sequence until all the predetermined perfusion reagents have completed the perfusion process.

[0085] 5) Insert the chip to be tested. As the chip is gradually inserted, the chip frame and the gasket fixing head physically interfere with each other. The external force of the chip frame causes the gasket fixing head to squeeze and deform the spring, causing relative displacement between the gasket fixing head and the inlet of the fluid manifold block, immediately breaking the original fluid connection between the two. As the chip to be tested continues to be inserted until it is in place, the inlet of the chip to be tested aligns with the reagent outlet on the fluid manifold block, establishing a fluid connection. The gasket fixing head retreats due to the deformation of the elastic body. The instrument then begins to perform related tasks such as pumping liquid, temperature control, and testing on the chip to be tested.

[0086] 6) After the chip is tested, the tested chip is taken out. During the removal process, the external force on the sealing gasket fixing head gradually disappears, causing the squeezed spring to gradually recover. The spring pushes the sealing gasket fixing head to move near the outlet end of the manifold block. At this time, the magnetic suction and clamping mechanism on the fluid manifold block applies a guiding force to the sealing gasket fixing head through magnetic attraction, so that the sealing gasket fixing head is aligned and concentric with the corresponding reagent outlet on the manifold block; then the clamping mechanism can cooperate with the sealing fastening device through magnetic attraction to form a downward pressure applied to the sealing ring, ensuring that the sealing gasket fixing head and the reagent outlet of the manifold block form a sealed fluid connection.

[0087] Example 2:

[0088] 1. Device structure:

[0089] The fluid manifold block adapted for a single-channel sequencing chip has a flow channel inlet, and a column with a pressing piece (ie, elastic pressing piece 64) fixed on each side of the flow channel inlet serves as a pressing mechanism.

[0090] The gasket retainer is a T-shaped PEI component with a flow channel inlet at one end that mates with the manifold block inlet and houses a rubber gasket. The T-shaped portion of the retainer has protrusions on either side of the gasket that act as a sealing and securing mechanism. A port at the end of the retainer provides a fluid connection to the pre-filled tubing. Internally, a flow channel connects the inlet gasket to the outlet connector. At the rear end of the retainer, a stepped reducer secures the retainer to one end of the rebound mechanism.

[0091] The mechanism fixing seat is a stainless steel pressure block with two screw holes on it for fixing the fixing seat to the fluid manifold block. There is a stepped through hole in the middle of the fixing block for passing the sealing gasket fixing seat. At the same time, the step in the through hole is also used to fix the other end of the elastomer.

[0092] A single-channel flow chamber sequencing biochip has a modified silicon inner wall on one side and quartz glass on the other. The silicon chip is surrounded by a chip frame, which has sealing gaskets embedded near the chip inlet and outlet ports.

[0093] The layout of the device in the instrument fluid system is similar to that of the embodiment 1. Figure 4 As shown, the reagent storage module is connected to the reagent switching module, the reagent switching module is connected to the inlet of the fluid manifold block, the pre-infusion structure is connected to the reagent drive unit, and the discharge outlet of the reagent drive unit is connected to the waste liquid collection module. At the same time, the outlet of the fluid manifold block is connected to another inlet of the reagent drive module.

[0094] 2. Reagent pre-infusion:

[0095] 1) Insert the reagent tank to be pre-filled, and the reagent tank is fluidically connected to the fluid manifold block through pipes, manifold blocks, reagent needles, etc. There is no need to insert the chip to be tested at this time.

[0096] 2) When there is no external force, the sealing gasket fixing head moves to the vicinity of the manifold block outlet due to the restoring force applied by the spring. At this time, the pressing mechanism on the fluid manifold block squeezes and deforms between the elastomer and the sealing gasket fixing head, forming a downward pressure applied to the sealing ring, ensuring that the sealing gasket fixing head and the manifold block reagent outlet form a sealed fluid connection.

[0097] 3) When the sealing gasket fixing head is in the pre-infusion position, the reagent storage module, the switching module, the fluid manifold block inlet, the pre-infusion mechanism, the reagent driving unit, and the waste liquid collection module form a fluid connection, and then the reagent driving unit works to drive the reagent to be infused through the pipeline and the sealing gasket into the sealing gasket fixing head and then into the pre-infusion pipeline at the rear end of the fixing head, thereby realizing the pre-infusion of this reagent in the pipeline in front of the fluid manifold block inlet.

[0098] 4) The reagent switching module works so that different reagents to be perfused repeat step 3 in sequence until all the predetermined perfusion reagents have completed the perfusion process.

[0099] 5) Insert the chip to be tested. As the chip is gradually inserted, the chip frame and the gasket fixing head physically interfere with each other. The external force of the chip frame causes the gasket fixing head to squeeze and deform the spring, causing relative displacement between the gasket fixing head and the inlet of the fluid manifold block, immediately breaking the original fluid connection between the two. As the chip to be tested continues to be inserted until it is in place, the inlet of the chip to be tested aligns with the reagent outlet on the fluid manifold block, establishing a fluid connection. The gasket fixing head retreats due to the deformation of the elastic body. The instrument then begins to perform related tasks such as pumping liquid, temperature control, and testing on the chip to be tested.

[0100] 6) After the chip is tested, the tested chip is taken out. During the removal process, the external force on the sealing gasket fixing head gradually disappears, causing the squeezed spring to gradually recover. The spring pushes the sealing gasket fixing head to move near the manifold block outlet. At this time, the pressing mechanism on the fluid manifold block squeezes and deforms between the elastomer and the sealing gasket fixing head, forming a downward pressure applied to the sealing ring, ensuring that the sealing gasket fixing head and the manifold block reagent outlet form a sealed fluid connection.

[0101] Example 3:

[0102] 1. Device structure:

[0103] A fluid manifold block adapted for a dual-channel sequencing chip has two inlet flow channels, and a cylindrical NdFeB magnet is installed in each pin hole on the outside of the flow channel inlet as a clamping mechanism.

[0104] The gasket retainer is a T-shaped PEI component with a flow channel inlet at one end that mates with the manifold block inlet and is fitted with a rubber gasket. A cylindrical NdFeB magnet is mounted in each pinhole on either side of the gasket as a sealing and securing device. The polarity of the magnet's lower surface is opposite to the polarity of its upper surface, ensuring mutual attraction between the two magnets. A port at the end of the retainer provides a fluid connection to the pre-filled tubing. Internally, a flow channel provides a fluid connection between the inlet gasket and the outlet connector. At the rear end of the retainer, a stepped reducer secures the retainer to one end of the rebound mechanism.

[0105] The mechanism fixing seat is a stainless steel pressure block with two screw holes on it for fixing the fixing seat to the fluid manifold block. There is a stepped through hole in the middle of the fixing block for passing the sealing gasket fixing seat. At the same time, the step in the through hole is also used to fix the other end of the elastomer.

[0106] A dual-channel flow cell sequencing biochip has a chemically modified silicon inner wall on one side and quartz glass on the other. The silicon chip is surrounded by a chip frame, which has sealing gaskets embedded near the chip's inlet and outlet ports.

[0107] In this device, the reagent storage module is connected to the reagent switching module, which is connected to the inlet of the fluid manifold block. The pre-infusion structure is connected to the reagent drive unit, and the discharge outlet of the reagent drive unit is connected to the waste liquid collection module. At the same time, the outlet of the fluid manifold block is connected to another inlet of the reagent drive module.

[0108] 2. Reagent pre-infusion:

[0109] 1) Insert the reagent tank to be pre-filled, and the reagent tank is fluidically connected to the fluid manifold block through pipes, manifold blocks, reagent needles, etc. There is no need to insert the chip to be tested at this time.

[0110] 2) In the absence of external force, the spring-loaded seal retainer moves to the vicinity of the manifold block outlet. The magnetic clamping mechanism on the fluid manifold block then magnetically attracts the magnetic seal fastening mechanism on the seal retainer, guiding the seal retainer to align and concentrically align with the corresponding reagent outlet on the manifold block. The attractive force between the two magnetic mechanisms then creates positive pressure on the seal ring, ensuring a sealed fluid connection between the seal retainer and the reagent outlet on the manifold block.

[0111] 3) When the sealing gasket fixing head is in the pre-infusion position, the reagent storage module, the switching module, the fluid manifold block inlet, the pre-infusion mechanism, the reagent driving unit, and the waste liquid collection module form a fluid connection, and then the reagent driving unit works to drive the reagent to be infused through the pipeline and the sealing gasket into the sealing gasket fixing head and then into the pre-infusion pipeline at the rear end of the fixing head, thereby realizing the pre-infusion of this reagent in the pipeline in front of the fluid manifold block inlet.

[0112] 4) The reagent switching module works so that different reagents to be perfused repeat step 3 in sequence until all the predetermined perfusion reagents have completed the perfusion process.

[0113] 5) Insert the chip to be tested. As the chip is gradually inserted, the chip frame and the gasket fixing head physically interfere with each other. The external force of the chip frame causes the gasket fixing head to squeeze and deform the spring, causing relative displacement between the gasket fixing head and the inlet of the fluid manifold block, immediately breaking the original fluid connection between the two. As the chip to be tested continues to be inserted until it is in place, the inlet of the chip to be tested aligns with the reagent outlet on the fluid manifold block, establishing a fluid connection. The gasket fixing head retreats due to the deformation of the elastic body. The instrument then begins to perform related tasks such as pumping liquid, temperature control, and testing on the chip to be tested.

[0114] 6) After the chip is tested, the tested chip is taken out. During the removal process, the external force on the sealing gasket fixing head gradually disappears, causing the squeezed spring to gradually recover. The spring pushes the sealing gasket fixing head to move near the outlet end of the manifold block. At this time, the magnetic suction and clamping mechanism on the fluid manifold block applies a guiding force to the sealing gasket fixing head through magnetic attraction, so that the sealing gasket fixing head is aligned and concentric with the corresponding reagent outlet on the manifold block; then the clamping mechanism can cooperate with the sealing fastening device through magnetic attraction to form a downward pressure applied to the sealing ring, ensuring that the sealing gasket fixing head and the reagent outlet of the manifold block form a sealed fluid connection.

[0115] Example 4:

[0116] 1. Device structure:

[0117] A fluid manifold block adapted for a single-channel sequencing chip has a flow channel inlet, and a cylindrical NdFeB magnet is installed in the pin holes on both sides of the flow channel inlet and outlet as a clamping mechanism.

[0118] The sealing gasket retainer is a T-shaped PEI component with a flow channel inlet at one end that mates with the manifold block inlet. A rubber sealing gasket is installed at the flow channel inlet. A cylindrical NdFeB magnet is installed in each pinhole on either side of the sealing gasket as a sealing and fastening device. At the end of the retainer, there is an interface for fluid connection with another sealing gasket retainer. Internally, a flow channel connects the inlet gasket of the retainer to the outlet connector. At the rear end of the retainer, a shaft-like mechanism connects to the shaft. Next to the shaft, a torsion spring acts as an elastic member. One side of the spring contacts the sealing gasket retainer, while the other side is fixed to the fluid manifold block.

[0119] A single-channel flow chamber sequencing biochip has a modified silicon inner wall on one side and quartz glass on the other. The silicon chip is surrounded by a chip frame, which has sealing gaskets embedded near the chip inlet and outlet ports.

[0120] The layout of the device is as follows Figure 14 As shown, the reagent storage module is connected to the reagent switching module, the reagent switching module is connected to the fluid manifold block inlet, and the pre-infusion mechanism 1 is connected to the pre-infusion mechanism 2. The fluid manifold block outlet is connected to the reagent drive unit, and the discharge outlet of the reagent drive unit is connected to the waste liquid collection module.

[0121] 2. Reagent pre-infusion:

[0122] 1) Insert the reagent tank to be pre-filled, and the reagent tank is fluidically connected to the fluid manifold block through pipes, manifold blocks, reagent needles, etc. There is no need to insert the chip to be tested at this time.

[0123] 2) When no external force is applied, the sealing head, due to the restoring torque applied by the torsion spring, moves to the vicinity of the manifold block inlet and outlet. At this point, the magnetic clamping mechanism on the fluid manifold block magnetically attracts the magnetic sealing fastening device on the sealing head, guiding the sealing head to align and concentric with the corresponding reagent outlet on the manifold block. The attractive force between the two magnetic mechanisms then creates downward pressure on the sealing ring, ensuring a sealed fluid connection between the sealing head and the manifold block reagent outlet.

[0124] 3) When the sealing gasket fixing head is in the pre-infusion position, the reagent storage module, the switching module, the fluid manifold block inlet, the pre-infusion mechanism 1, the pre-infusion mechanism 2 (i.e., the two pre-infusion parts), the fluid manifold block outlet, the reagent driving unit, and the waste liquid collection module form a fluid connection, and then the reagent driving unit works to drive the reagent to be infused through the pipeline and the sealing gasket into the sealing gasket fixing head and then into the pre-infusion pipeline at the rear end of the fixing head, thereby realizing the pre-infusion of this reagent in the pipeline in front of the fluid manifold block inlet.

[0125] 4) The reagent switching module works so that different reagents to be perfused repeat step 3 in sequence until all the predetermined perfusion reagents have completed the perfusion process.

[0126] 5) Insert the chip to be tested. As the chip to be tested is gradually inserted, the chip frame and the sealing head form physical interference. Under the external force of the chip frame, the sealing head squeezes and deforms the torsion spring, causing relative displacement between the sealing head and the inlet and outlet of the fluid manifold block, and the original fluid connection between the two is immediately broken. When the chip to be tested is further inserted until it is in place, the inlet of the chip to be tested aligns with the reagent outlet on the fluid manifold block and establishes a fluid connection, while the sealing head retreats due to the deformation of the elastic body. The instrument then begins to perform related operations such as pumping liquid, temperature control, and testing on the chip to be tested.

[0127] 6) After the chip is tested, the tested chip is taken out. During the removal process, the external force on the sealing gasket fixing head gradually disappears, causing the squeezed torsion spring to gradually recover. The torsion spring pushes the sealing gasket fixing head to move near the inlet and outlet ends of the manifold block. At this time, the magnetic clamping mechanism on the fluid manifold block applies a guiding force to the sealing gasket fixing head through magnetic attraction, so that the sealing gasket fixing head is aligned and concentric with the corresponding reagent outlet on the manifold block; then the clamping mechanism can cooperate with the sealing fastening device through magnetic attraction to form a downward pressure applied to the sealing ring to ensure that the sealing gasket fixing head and the reagent outlet of the manifold block form a sealed fluid connection.

[0128] The technical problems to be solved by this invention include: 1) reducing air residue in the pipeline after reagent pre-infusion, thereby ensuring that the quality of the sample to be tested is not affected by bubbles. 2) reducing the use of high-cost solenoid valves, thereby reducing the cost of the mechanism. 3) using a simple and reliable structure to improve the reliability of the mechanism. 4) further simplifying the user operation process and improving the user experience.

[0129] The beneficial effects of the present invention are as follows: 1) There is no need to insert a discarded chip during the pre-infusion process, which reduces user workload, lowers the risk of human error, and improves the user experience. 2) The pre-infusion process can pre-infuse all pipelines before the chip inlet, so that after the infusion is completed and the chip is placed, no reagents containing bubbles will be infused into the chip, effectively avoiding the impact of bubbles on sample detection quality. 3) The overall mechanism does not contain high-cost components such as solenoid valves, making the mechanism cost lower. 4) This mechanism has a simple structure and high reliability.

[0130] The foregoing description is merely a preferred embodiment of the present invention and is not intended to limit the present invention. Those skilled in the art will readily appreciate that various modifications and variations of the present invention are possible. Any modifications, equivalent substitutions, or improvements made within the spirit and principles of the present invention are intended to be within the scope of protection of the present invention.

Claims

1. A gene sequencing device, characterized in that: include: A pre-filling mechanism, the pre-filling mechanism comprising a base (11) and a first pre-filling portion (20), the base (11) having a first flow channel (12), the first pre-filling portion (20) having a second flow channel (21), the first pre-filling portion (20) being movable to a first pre-filling position or a first storage position, wherein when the first pre-filling portion (20) is in the first pre-filling position, the first flow channel (12) and the second flow channel (21) are connected, and when the first pre-filling portion (20) is in the first storage position, the first flow channel (12) and the second flow channel (21) are disconnected; a reagent driving portion (31), used for driving the reagent to flow, wherein the reagent driving portion (31) is in communication with the first flow channel (12); A flow pool (40), wherein the flow pool (40) has a third flow channel (41), and the flow pool (40) is movable to a connection position. When the flow pool (40) is in the connection position, the first pre-filling portion (20) is in the first storage position, and the third flow channel (41) is in communication with the first flow channel (12); The flow pool (40) can also be moved to a separation position. When the flow pool (40) is in the separation position, the third flow channel (41) and the first flow channel (12) are disconnected, and the first pre-filling portion (20) is in the first pre-filling position. A rebound mechanism (50) applies elastic force to the first pre-filling portion (20), and when the flow pool (40) moves to the connection position, the flow pool (40) pushes the first pre-filling portion (20) to move to the first storage position; when the flow pool (40) leaves the connection position, the rebound mechanism (50) pushes the first pre-filling portion (20) to move to the first pre-filling position.

2. The gene sequencing device according to claim 1, characterized in that The pre-infusion mechanism also includes: A sealing mechanism (60) applies a force on the first pre-filling portion (20) toward the base (11) when the first pre-filling portion (20) is in the first pre-filling position.

3. The gene sequencing device according to claim 2, characterized in that The sealing mechanism (60) comprises: A first magnetic attraction member (61) is arranged on the base (11); The second magnetic member (62) is arranged on the first pre-filling portion (20), and when the first pre-filling portion (20) is in the first pre-filling position, the first magnetic member (61) and the second magnetic member (62) are attracted to each other.

4. The gene sequencing device according to claim 3, characterized in that: The sealing mechanism (60) comprises: A connecting member (63) is provided on the base (11); The elastic pressing piece (64) is connected to the connecting piece (63), and when the first pre-filling portion (20) is in the first pre-filling position, the elastic pressing piece (64) presses the first pre-filling portion (20).

5. The gene sequencing device according to claim 1, characterized in that: The pre-infusion mechanism also includes: a first sealing ring (15) disposed around the opening of the second flow channel (21); a second sealing ring (16) disposed around the opening of the third flow channel (41); Wherein, when the first pre-filling portion (20) is in the first pre-filling position, the first sealing ring (15) and the opening of the first flow channel (12) are butted against each other; when the flow pool (40) is in the connection position, the second sealing ring (16) and the opening of the first flow channel (12) are butted against each other.

6. The gene sequencing device according to claim 1, characterized in that The base (11) has a plurality of the first flow channels (12), the second flow channels (21) have a plurality of openings, and the flow pool (40) has a plurality of the third flow channels (41); wherein, when the first pre-filling portion (20) is in the first pre-filling position, the plurality of the first flow channels (12) and the plurality of the openings of the second flow channels (21) are connected in a one-to-one correspondence; and when the flow pool (40) is in the connection position, the plurality of the first flow channels (12) and the plurality of the third flow channels (41) are connected in a one-to-one correspondence.

7. The gene sequencing device according to claim 1, characterized in that The base (11) further comprises a fourth flow channel (17), wherein the fourth flow channel (17) and the first flow channel (12) are spaced apart from each other. The pre-filling mechanism further comprises a second pre-filling portion (70), wherein the second pre-filling portion (70) comprises a fifth flow channel (71), wherein the fifth flow channel (71) is connected to the second flow channel (21); the second pre-filling portion (70) can be moved to a second pre-filling position or a second storage position, wherein when the second pre-filling portion (70) is in the second pre-filling position, the fourth flow channel (17) is connected to the fifth flow channel (71); when the second pre-filling portion (70) is in the second storage position, the fourth flow channel (17) and the fifth flow channel (71) are disconnected; when the flow pool (40) is in the connection position, the second pre-filling portion (70) is in the second storage position, and both ends of the third flow channel (41) are connected to the first flow channel (12) and the fourth flow channel (17), respectively.

8. The gene sequencing device according to claim 7, characterized in that: The flow pool (40) can also be moved to a separation position. When the flow pool (40) is in the separation position, the third flow channel (41) and the first flow channel (12) and the fourth flow channel (17) are all disconnected, the first pre-filling portion (20) is in the first pre-filling position, and the second pre-filling portion (70) is in the second pre-filling position. The pre-filling mechanism further comprises a hose (18), and the fifth flow channel (71) and the second flow channel (21) are connected via the hose (18); when the flow pool (40) moves to the connection position, the flow pool (40) pushes the first pre-filling part (20) to move to the first storage position, and the flow pool (40) pushes the second pre-filling part (70) to move to the second storage position.

Images