Automated device and automated detection method for air sampling and detection

Abstract

The present invention discloses an automated device and automated detection method for air sampling and detection, wherein the automated device for air sampling and detection comprises a housing, a collection module, a pretreatment module, a manipulator, a liquid transfer module, a detection module and a recovery module. The collection, pretreatment and detection of air are achieved through the cooperation between different modules without manual processing, and the technical solution of the present invention can improve the efficiency of environmental air detection.

Description

Technical Field

[0001] The present invention relates to the technical field of air detection, and in particular to an automated device and an automated detection method for air sampling and detection. Background Art

[0002] There are many components in the air, such as microorganisms, bioaerosols and large particles. Bioaerosols include biological particles such as fungi, bacteria, and viruses, while large particles include PM2.5 and PM1.0. All of the above substances may affect human health, and accurate judgment of the surrounding environment can provide improvement plans in a timely manner.

[0003] In the prior art, there are rapid detection devices, which can only directly collect samples through swabs or sensors and detect them through small independent detection boxes, with a small number of tests and low detection accuracy. In addition, the collection capacity of the swab is limited and cannot accurately represent the specific conditions of the environment in which the object to be detected is located. Summary of the invention

[0004] The main purpose of the present invention is to provide an automated device for air sampling and detection, which aims to realize automatic integrated equipment for batch operations such as air collection, pretreatment and detection, and continuously collect and detect multiple samples at the same time, thereby improving the accuracy of environmental air detection and the detection efficiency.

[0005] To achieve the above-mentioned purpose, the automatic device for air sampling and detection proposed by the present invention comprises:

[0006] The shell is provided with a working chamber, the bottom of the working chamber is provided with a mounting bracket, and the top of the working chamber is provided with a movable bracket;

[0007] A pretreatment module is arranged in the working chamber and mounted on the mounting bracket. The pretreatment module comprises a storage device and a pretreatment device. The storage device stores at least a first reagent, a second reagent, a third reagent and a sampling container. The mounting bracket has a pretreatment position. The pretreatment device is arranged at the pretreatment position.

[0008] A collection module is provided in the working chamber and mounted on the mounting bracket. The collection module is used to collect air and collect the objects to be detected in the air into the sampling container. The collection module has a collection position, and the shell is exposed with a collection port. The collection port is used to collect the objects to be detected in the air. The pre-processing module can move the sampling container back and forth between the collection position and the pre-processing position.

[0009] A manipulator is provided in the working chamber and mounted on the movable bracket. The sampling container is provided with a first cover. The manipulator is at least used to move the sampling container to the pretreatment device and to tighten or loosen the first cover and the sampling container.

[0010] a liquid transfer module, disposed in the working chamber and mounted on the movable bracket, the liquid transfer module being used to transfer the first reagent, the second reagent or the third reagent;

[0011] a detection module, disposed in the working chamber and mounted on the mounting bracket, the detection module being used to detect the object to be detected; and

[0012] A recovery module is provided in the working chamber and installed on the mounting bracket. The recovery module is used to recover the used first reagent, the second reagent, the third reagent and the sampling container.

[0013] Optionally, the pretreatment device includes a container holder, a container moving mechanism and a container lifting mechanism; the container holder is arranged on the container moving mechanism, one end of the container moving mechanism is arranged at the pretreatment position, and the other end is arranged at the collection position, and the container moving mechanism can drive the container holder to move to the pretreatment position or the collection position; the container lifting mechanism is arranged at the collection position, and when the container holder moves to the collection position, the container lifting mechanism 313 lifts the container holder so that the collection container in the container holder rises to the collection module.

[0014] Optionally, the storage device includes a first storage area storing a plurality of TIP heads; a second storage area storing a sampling container, the sampling container being pre-added with the first reagent; and a third storage area storing a second reagent and a third reagent.

[0015] Optionally, the recycling module includes a first waste box, a second waste box and a third waste box arranged on the mounting bracket; the first waste box is arranged in the first storage area for recycling the used TIP head; the second waste box is arranged in the second storage area for recycling the used sampling container; the third waste box is arranged close to the detection module for recycling the reagent bottle after detection.

[0016] Optionally, the third waste box is provided with an inversion device; the inversion device comprises an inversion bracket and an inversion motor, the inversion bracket is used to accommodate the reagent bottle, and the inversion motor drives the inversion bracket to rotate at least 90 degrees toward the direction of the third waste box; the second waste box is provided with a waste port, and the waste port is provided with a guiding slope extending toward the interior of the second waste box.

[0017] Optionally, the detection module includes an air detection mechanism and an automatic flip-cover device, the air detection mechanism is provided with a receiving groove, and the automatic flip-cover structure can be rotatably covered on the receiving groove to form a sealed detection space in the receiving groove; the air detection mechanism can detect the objects to be detected collected from the air by fluorescent labeling method.

[0018] Optionally, the collection module is an air sampling device, which includes a fan box, an air duct and a centrifugal part; the fan box is connected to the centrifugal part through the air duct, an air inlet is provided on the side of the centrifugal part, and the air inlet is connected to the collection port, and the bottom of the centrifugal part has a downwardly arranged collection hole, and the opening of the sampling container can abut against the collection hole.

[0019] Optionally, the automated air sampling and detection device further comprises a protective cover device, wherein the protective cover device comprises a protective cover body and a cover body motor, and the cover body motor can rotate and drive the protective cover body to cover the collection port.

[0020] Optionally, the movable bracket includes a horizontal moving mechanism and a mounting member connected to the horizontal moving mechanism; a first horizontal moving mechanism and a second horizontal moving mechanism are provided on the mounting member, the pipetting module is provided on the first horizontal moving mechanism, and the manipulator is provided on the second horizontal moving mechanism.

[0021] The present invention also provides an automated detection method, including the aforementioned automated air sampling and detection device, the automated air sampling and detection device also including at least a second reagent bottle and a third reagent bottle, the second reagent bottle storing the second reagent and having a second cover body covering the second reagent bottle, the third reagent bottle storing the third reagent and having a third cover body covering the third reagent bottle;

[0022] The automated air sampling and detection device adopts the automated detection method, which includes the following steps:

[0023] The manipulator moves to the storage device;

[0024] The first reagent is pre-added in the sampling container, and the manipulator grabs the sampling container in the storage device and moves it to the pre-treatment position;

[0025] The pretreatment device fixes the sampling container, and the manipulator loosens and removes the first cover from the sampling container;

[0026] The pre-processing device moves the sampling container from the pre-processing position to the collection position;

[0027] The collection module sucks air from the collection port and collects the objects to be detected in the air into the sampling container;

[0028] The pre-processing device moves the sampling container collecting the object to be detected to the pre-processing position;

[0029] The manipulator grabs the second reagent bottle in the storage device and moves it to the pretreatment position; the pretreatment device fixes the second reagent bottle, and the manipulator loosens and removes the second cover from the second reagent bottle;

[0030] The manipulator grabs the third reagent bottle in the storage device and moves it to the pretreatment position; the pretreatment device fixes the third reagent bottle, and the manipulator loosens and removes the third cover from the third reagent bottle;

[0031] The pipetting module moves the first reagent and the object to be detected in the sampling container to the second reagent in the second reagent bottle and mixes them evenly; the pipetting module then moves the first reagent, the second reagent and the object to be detected mixed in the second reagent bottle to the third reagent in the third reagent bottle, thereby obtaining a final liquid to be detected;

[0032] The manipulator covers the first cover body on the sampling container, covers the second cover body on the second reagent bottle, covers the third cover body on the third reagent bottle, and moves the third reagent bottle to the detection module for detection;

[0033] The manipulator moves the second reagent bottle and the sampling container in the pretreatment position and the third reagent bottle in the detection module to the recovery module.

[0034] The technical solution of the present invention realizes automatic collection of air and collects the objects to be detected in the air through the collection module for processing and detection by subsequent modules, and transfers the collected objects to be processed to the pretreatment position and adds reagents through the pretreatment module, the manipulator and the pipetting module. The manipulator can move the reagent bottle where the pretreated object to be detected is located to the detection module for detection and obtain the results. In order to meet the needs of higher automation, the automatic device for air sampling and detection is also provided with a recovery module, which is arranged in the working chamber and fixedly installed on the mounting bracket. The recovery module can recover the first reagent, the second reagent, the third reagent and the sampling container, so that the next round of sampling, pretreatment and detection operations can be carried out smoothly. It should be noted that the equipment of this embodiment integrates multiple steps such as collection, pretreatment and detection, and detects multiple samples simultaneously and continuously, with high detection efficiency, to form an uninterrupted detection of ambient air, thereby making the detection results more accurate. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the structures shown in these drawings without paying creative work.

[0036] Figure 1 It is a structural schematic diagram of an embodiment of an automated device for air sampling and detection of the present invention;

[0037] Figure 2 A schematic diagram of the internal structure of an embodiment of an automated device for air sampling and detection according to the present invention;

[0038] Figure 3 A schematic structural diagram of an embodiment of a manipulator and a liquid transfer module of an automated device for air sampling and detection of the present invention;

[0039] Figure 4 It is a structural schematic diagram of an embodiment of a pre-processing module, a collection module, a detection module and a recovery module of an automated device for air sampling and detection of the present invention;

[0040] Figure 5 It is a structural schematic diagram of a pre-processing module of the automated device for air sampling and detection of the present invention;

[0041] Figure 6 A schematic diagram of the structure of the container lifting mechanism of the pre-processing module of the automated device for air sampling and detection of the present invention;

[0042] Figure 7A schematic diagram of the structure of the container support of the automated device for air sampling and detection of the present invention;

[0043] Figure 8 A schematic diagram of the structure of a processing station of the automated device for air sampling and detection of the present invention;

[0044] Fig. 9 A schematic diagram of the mechanism of the protective cover device of the automated device for air sampling and detection of the present invention;

[0045] Fig.10 It is a schematic diagram of the structure of the second waste box and the third waste box of the automated device for air sampling and detection of the present invention;

[0046] Fig.11 It is a structural schematic diagram of the inverting device of the automatic device for air sampling and detection of the present invention;

[0047] Fig.12 It is a structural schematic diagram of a detection module of the automated device for air sampling and detection of the present invention;

[0048] Fig.13 It is a structural schematic diagram of the automatic cover flipping device of the automatic device for air sampling and detection of the present invention.

[0049] Description of Figure Numbers:

[0050]

[0051] The realization of the purpose, functional features and advantages of the present invention will be further explained in conjunction with embodiments and with reference to the accompanying drawings. DETAILED DESCRIPTION

[0052] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] It should be noted that all directional indications (such as up, down, left, right, front, back, etc.) in the embodiments of the present invention are only used to explain the relative position relationship, movement status, etc. between the components under a certain specific posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

[0054] In the present invention, unless otherwise clearly specified and limited, the terms "connection", "fixation", etc. should be understood in a broad sense. For example, "fixation" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements, unless otherwise clearly defined. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0055] In addition, if there are descriptions involving "first", "second", etc. in the embodiments of the present invention, the descriptions of "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Therefore, the features defined as "first" and "second" may explicitly or implicitly include at least one of the features. In addition, the meaning of "and / or" appearing in the full text includes three parallel schemes. Taking "A and / or B" as an example, it includes scheme A, or scheme B, or a scheme that satisfies both A and B. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on the ability of ordinary technicians in the field to implement. When the combination of technical solutions is contradictory or cannot be implemented, it should be deemed that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0056] The invention provides an automatic device for air sampling and detection.

[0057] Reference Figures 1 to 13 In one embodiment of the present invention, the automated air sampling and detection device comprises:

[0058] The housing 100 is provided with a working chamber, wherein a mounting bracket 1 is provided at the bottom of the working chamber and a movable bracket 2 is provided at the top;

[0059] A pretreatment module 3 is provided in the working chamber and mounted on the mounting bracket 1. The pretreatment module 3 includes a storage device 32 and a pretreatment device 31. The storage device 32 stores at least a first reagent, a second reagent, a third reagent and a sampling container 321. The mounting bracket 1 has a pretreatment position, and the pretreatment device 31 is provided at the pretreatment position.

[0060] A collection module is provided in the working chamber and mounted on the mounting bracket 1. The collection module is used to collect air and collect the objects to be detected in the air into the sampling container 321. The collection module has a collection position. The housing 100 is exposed with a collection port 45. The collection port 45 is used to collect the objects to be detected in the air. The pre-processing module 3 can move the sampling container 321 back and forth between the collection position and the pre-processing position.

[0061] A manipulator 5 is provided in the working chamber and mounted on the movable bracket 2. The sampling container 321 is provided with a first cover. The manipulator 5 is at least used to move the sampling container 321 to the pretreatment device 31 and to tighten or loosen the first cover and the sampling container 321.

[0062] A pipetting module 6, disposed in the working chamber and mounted on the movable bracket 2, the pipetting module 6 being used to move the first reagent, the second reagent or the third reagent;

[0063] A detection module 7, disposed in the working chamber and mounted on the mounting bracket 1, the detection module 7 being used to detect the object to be detected; and

[0064] The recovery module 8 is disposed in the working chamber and mounted on the mounting bracket 1 . The recovery module 8 is used to recover the used first reagent, the second reagent, the third reagent and the sampling container 321 .

[0065] The technical solution of the present invention realizes automatic collection of air and collects the objects to be detected in the air through the collection module for processing and detection by subsequent modules, and transfers the collected objects to be processed to the pretreatment position and adds reagents through the pretreatment module 3, the manipulator 5 and the pipetting module 6. The manipulator 5 can move the reagent bottle where the pretreated object to be detected is located to the detection module 7 for detection and obtain results. In order to meet the higher automation requirements, the automatic device for air sampling and detection is also provided with a recovery module 8, which is arranged in the working chamber and fixedly installed on the mounting bracket 1. The recovery module 8 can recover the first reagent, the second reagent, the third reagent and the sampling container 321, so that the next round of sampling, pretreatment and detection operations can be carried out smoothly. It should be noted that the equipment of this embodiment integrates multiple steps such as collection, pretreatment and detection, and detects multiple samples simultaneously and continuously, with high detection efficiency, to form an uninterrupted detection of ambient air, thereby making the detection results more accurate.

[0066] The automated air sampling and detection device includes a plurality of modules that cooperate to realize the process of collecting, processing and detecting microorganisms or bioaerosols or atmospheric particulate matter in the air.

[0067] First, the collection module in the automated device for air sampling and detection is introduced. The collection module is arranged in the working chamber and fixedly mounted on the mounting bracket 1. The collection module can be used to collect the air of the surrounding environment and collect substances in the air.

[0068] In this embodiment, the shell 100 reveals a collection port 45, and the collection module has a collection position. The collection port 45 is used to collect objects to be detected in the air. A sampling container 321 is placed in the collection position. The air processed by the collection module can collect the objects to be detected in the air in the sampling container 321.

[0069] Specifically, the collection module is an air sampling device 4, which includes a fan box 41, an air duct 42 and a centrifugal part 43. An air inlet 44 is provided on the side of the centrifugal part 43. Since the fan in the fan box 41 provides suction, air enters the air inlet 44 of the centrifugal part 43 from the collection port 45. Under the centrifugal action of the centrifugal part 43, microorganisms, bioaerosols or atmospheric particles in the air will enter the sampling container 321 from the collection hole 47 set at the bottom of the centrifugal part 43. It should be noted that the collection hole 47 is set at the bottom of the centrifugal part 43 and is set downward, and the sampling container 321 is abutted against the collection hole 47.

[0070] Furthermore, in this embodiment, the air sampling and detection automation device also includes a protective cover device 46, and the protective cover device 46 includes a protective cover body 461 and a cover body motor 462, and the cover body motor 462 can rotate and drive the protective cover body 461 to cover the collection port 45. Since the air sampling and detection automation device in this embodiment, after completing the collection action, avoids the external environment from continuously interfering with the air sampling and detection automation device, a protective cover device 46 is provided at the collection port 45, and the protective cover body 461 is driven to cover the collection port 45 by the cover body motor 462, thereby preventing other air from entering the collection module, thereby affecting the accuracy of the detection.

[0071] The second thing that needs to be introduced is the pretreatment module 3. The object to be detected in the aforementioned sampling container 321 cannot be detected directly, but needs to go through a certain process, add specific reagents and be processed before it can be detected in the detection module 7.

[0072] In this embodiment, the preprocessing module 3 includes a storage device 32 and a preprocessing device 31 .

[0073] The storage device 32 stores at least three reagents. Since three reagents are used in this embodiment, the three reagents are named as the first reagent, the second reagent and the third reagent respectively. However, it should be noted that the three reagents in this embodiment are all reagents required for ATP fluorescence detection.

[0074] Specifically, the storage device 32 is fixedly arranged on the mounting bracket 1, and the storage device 32 includes a first storage area 321, and the first storage area 321 stores a plurality of TIP heads for the pipetting module 6 to replace the TIP heads, so that the entire device can prevent the contamination of the reagent during the automated pipetting process, thereby affecting the detection result. The storage device 32 also includes a second storage area 322, and the second storage area 322 stores a sampling container 321, wherein the sampling container 321 is pre-added with a first reagent. The storage device 32 also includes a third storage area 323, and the third storage area 323 stores a second reagent and a third reagent, wherein the second reagent is contained in a second reagent bottle, and the second reagent bottle is provided with a second cover, and the third reagent is contained in the third reagent bottle, and the third reagent bottle is provided with a third cover. The setting of the cover can effectively prevent the volatilization and diffusion of the reagent.

[0075] The pre-processing device 31 includes a container moving mechanism 312 , a container lifting mechanism 313 , a container support 311 and a processing table 314 .

[0076] The container holder 311 is arranged on the container moving mechanism 312. One end of the container moving mechanism 312 is arranged on the pre-treatment position, and the other end is arranged on the collection position. The container moving mechanism 312 can drive the container holder 311 to move to the pre-treatment position or the collection position. The container lifting mechanism 313 is arranged on the collection position. When the container holder 311 moves to the collection position, the container lifting mechanism 313 lifts the container holder 311 so that the sampling container 321 in the container holder 311 rises to the collection module. The processing table 314 is arranged on the pre-treatment position. The container holder 311 is arranged on the container moving mechanism 312. The container holder 311 is provided with a receiving groove to accommodate the sampling container 321.

[0077] Specifically, the processing table 314 is provided with a first clamping portion 3141, the processing table 314 is provided with a first slide groove, and the first clamping portion 3141 can be slidably arranged in the first slide groove; the container support 311 is also provided with a second clamping portion 3116 and a second slide groove, and the second clamping portion 3116 can be slidably arranged in the second slide groove. The first clamping portion 3141 and the second clamping portion 3116 are separated from each other, and the container moving mechanism 312 can drive the container support 311 to abut against the processing table 314, the first clamping portion 3141 abuts against the second clamping portion 3116, and the first clamping portion 3141 moves toward the first slide groove to clamp the reagent bottle; the second clamping portion 3116 moves toward the second slide groove to clamp the sampling container 321. When the first clamping part 3141 clamps the reagent bottle, the second clamping part 3116 clamps the sampling container 321. In this way, the manipulator 5 with rotatable jaws can tighten or loosen the covers of the second reagent bottle, the third reagent bottle and the sampling container 321 in the pretreatment position.

[0078] The container moving mechanism 312 is a screw mechanism to drive the container holder 311 disposed on the screw mechanism to reciprocate between the pre-treatment position and the collection position. It should be noted that the collection position is also a top-lifting position, and the container lifting mechanism 313 is disposed near the top-lifting position. The container lifting mechanism 313 is provided with another set of screw mechanisms. The container lifting mechanism 313 is provided with an abutment portion extending toward the top-lifting position. The abutment portion can abut against the container holder 311 to move upward in the height direction, thereby causing the opening of the sampling container 321 in the container holder 311 to abut against the collection hole 47 of the centrifugal part 43.

[0079] Furthermore, the pipetting module 6 is arranged in the working chamber and installed on the mobile bracket 2, and the pipetting module 6 is used to move the first reagent, the second reagent and the third reagent. It should be noted that the pipetting module 6 has a pipette tip, and the pipette tip can be installed with a TIP head in the first storage area 321, and the pipetting module 6 is also provided with a push sleeve, and the push sleeve can move downward along the direction of the pipette tip, so that the TIP head arranged on the pipette tip falls off, and the pipette tip can be installed with a new TIP head.

[0080] The manipulator 5 is disposed in the working chamber and installed on the movable bracket 2. The sampling container 321 is provided with a first cover body. The manipulator 5 includes a rotatable clamping claw. The first cover body can be tightened or loosened by clamping the first cover body with the clamping claw and then rotating.

[0081] It should be noted that the mobile bracket 2 is a planar mobile mechanism, and the mobile bracket 2 includes two sets of screw mechanisms, and the movement directions of the two sets of screw mechanisms are perpendicular to each other. In order to make the space more compact, the screw and the motor are connected by a pulley, which reduces the length occupied by the screw mechanism in the space. Specifically, the mobile bracket 2 includes a horizontal moving mechanism and a mounting member connected to the horizontal moving mechanism; the mounting member is provided with a first horizontal moving mechanism 21 and a second horizontal moving mechanism 22, the pipetting module 6 is provided on the first horizontal moving mechanism 21, and the manipulator 5 is provided on the second horizontal moving mechanism 22.

[0082] Furthermore, the recycling module 8 is used to recycle reagent bottles, reagents, TIP heads and sampling containers 321, and the recycling module 8 includes a first waste box 81, a second waste box 82 and a third waste box 83 arranged on the mounting bracket 1; the first waste box 81 is arranged in the first storage area 321, and is used to recycle the used TIP heads; the second waste box 82 is arranged in the second storage area 322, and is used to recycle the used sampling containers 321; the third waste box 83 is arranged close to the detection module 7, and is used to recycle the reagent bottles after detection.

[0083] In one embodiment, the third waste box 83 is provided with an inversion device 84; the inversion device 84 includes an inversion bracket 841 and an inversion motor 842, the inversion bracket 841 is used to accommodate the reagent bottle, and the inversion motor 842 drives the inversion bracket 841 to rotate at least 90 degrees toward the direction of the third waste box 83; the second waste box 82 is provided with a waste port 85, and the waste port 85 is provided with a guiding slope 86 extending toward the interior of the second waste box 82.

[0084] As for the detection module 7, in this embodiment, the detection module 7 includes an air detection mechanism 71 and an automatic flip cover device 72, the air detection mechanism 71 is provided with a receiving groove 73, and the automatic flip cover structure can be rotatably covered on the receiving groove 73, so that a sealed detection space is formed in the receiving groove 73; the air detection mechanism 71 can detect the object to be detected collected from the air by fluorescent labeling. Specifically, in this embodiment, the air detection mechanism 71 includes a photoelectric detection module 7 and a sample receiving structure, and the photoelectric detection module 7 includes an electric control board, an excitation light source and a photomultiplier tube, and the electric control board is electrically connected to the photomultiplier tube. The excitation light source irradiates the sample receiving structure, and the photoelectric detection module 7 collects the background value of the object to be detected in the sample receiving structure, thereby detecting the object to be detected.

[0085] The automatic cover flipping device 72 comprises a cover 721, a cover flipping motor 722, a return spring and a cover rod 723. The cover flipping motor 722 drives the cover rod 723 to rotate, thereby pushing the cover 721 to cover the air detection mechanism 71. The return of the cover 721 is achieved by the return spring.

[0086] The present invention also proposes an automated detection method, which includes the automated device for air sampling and detection. The specific structure of the automated device for air sampling and detection refers to the above-mentioned embodiment. Since this automated detection method adopts all the technical solutions of all the above-mentioned embodiments, it has at least all the beneficial effects brought by the technical solutions of the above-mentioned embodiments, which will not be described one by one here.

[0087] Wherein, the automated air sampling and detection device further comprises at least a second reagent bottle and a third reagent bottle, wherein the second reagent bottle stores the second reagent and is provided with a second cover body for covering the second reagent bottle, and the third reagent bottle stores the third reagent and is provided with a third cover body for covering the third reagent bottle;

[0088] The automated air sampling and detection device adopts the automated detection method, which includes the following steps:

[0089] The robot 5 moves to the storage device 32;

[0090] The first reagent is pre-added in the sampling container 321, and the manipulator 5 grabs the sampling container 321 in the storage device 32 and moves it to the pre-treatment position;

[0091] The pre-treatment device 31 fixes the sampling container 321, and the manipulator 5 unscrews and removes the first cover from the sampling container 321;

[0092] The pre-processing device 31 moves the sampling container 321 from the pre-processing position to the collection position;

[0093] The collection module sucks air from the collection port 45 and collects the objects to be detected in the air into the sampling container 321;

[0094] The pre-processing device 31 moves the sampling container 321 collecting the object to be detected to the pre-processing position;

[0095] The manipulator 5 grabs the second reagent bottle in the storage device 32 and moves it to the pretreatment position; the pretreatment device 31 fixes the second reagent bottle, and the manipulator 5 loosens and removes the second cover from the second reagent bottle;

[0096] The manipulator 5 grabs the third reagent bottle in the storage device 32 and moves it to the pretreatment position; the pretreatment device 31 fixes the third reagent bottle, and the manipulator 5 loosens and removes the third cover from the third reagent bottle;

[0097] The pipetting module 6 moves the first reagent and the object to be detected in the sampling container 321 to the second reagent in the second reagent bottle and mixes them evenly; the pipetting module 6 then moves the first reagent, the second reagent and the object to be detected mixed in the second reagent bottle to the third reagent in the third reagent bottle, thereby obtaining the final liquid to be detected;

[0098] The manipulator 5 covers the first cover body on the sampling container 321, covers the second cover body on the second reagent bottle, covers the third cover body on the third reagent bottle, and moves the third reagent bottle to the detection module 7 for detection;

[0099] The robot 5 moves the second reagent bottle and the sampling container 321 in the pretreatment position and the third reagent bottle in the detection module 7 to the recovery module 8 .

[0100] The above descriptions are only optional embodiments of the present invention, and are not intended to limit the patent scope of the present invention. All equivalent structural changes made using the contents of the present invention's specification and drawings, or directly / indirectly applied in other related technical fields, are included in the patent protection scope of the present invention.

Claims

1. An automated device for air sampling and detection, characterized in that: include: The shell is provided with a working chamber, the bottom of the working chamber is provided with a mounting bracket, and the top of the working chamber is provided with a movable bracket; A pretreatment module is arranged in the working chamber and mounted on the mounting bracket. The pretreatment module comprises a storage device and a pretreatment device. The storage device stores at least a first reagent, a second reagent, a third reagent and a sampling container. The mounting bracket has a pretreatment position. The pretreatment device is arranged at the pretreatment position. A collection module is provided in the working chamber and mounted on the mounting bracket. The collection module is used to collect air and collect the objects to be detected in the air into the sampling container. The collection module has a collection position, and the shell is exposed with a collection port. The collection port is used to collect the objects to be detected in the air. The pre-processing module can move the sampling container back and forth between the collection position and the pre-processing position. A manipulator is provided in the working chamber and mounted on the movable bracket. The sampling container is provided with a first cover. The manipulator is at least used to move the sampling container to the pretreatment device and to tighten or loosen the first cover and the sampling container. a liquid transfer module, disposed in the working chamber and mounted on the movable bracket, the liquid transfer module being used to transfer the first reagent, the second reagent or the third reagent; A detection module, disposed in the working chamber and mounted on the mounting bracket, the detection module being used to detect the object to be detected; as well as A recovery module, disposed in the working chamber and mounted on the mounting bracket, the recovery module being used to recover the used first reagent, the second reagent, the third reagent and the sampling container; The pretreatment device includes a container support, a container moving mechanism and a container lifting mechanism; The container holder is arranged on the container moving mechanism, one end of the container moving mechanism is arranged at the pretreatment position, and the other end is arranged at the collection position, and the container moving mechanism can drive the container holder to move to the pretreatment position or the collection position; The container lifting mechanism is disposed at the collection position, and when the container holder moves to the collection position, the container lifting mechanism lifts the container holder so that the sampling container in the container holder rises to the collection module; The detection module includes an air detection mechanism and an automatic flip-cover device. The air detection mechanism is provided with a receiving groove. The automatic flip-cover device can be rotatably covered on the receiving groove to form a sealed detection space in the receiving groove. The air detection mechanism can detect the objects to be detected collected from the air by fluorescent labeling method.

2. The automated air sampling and detection device according to claim 1, characterized in that: The storage device includes a first storage area storing a plurality of TIP headers; a second storage area storing a sampling container, wherein the first reagent is pre-added in the sampling container; The third storage area stores the second reagent and the third reagent.

3. The automated air sampling and detection device according to claim 2, characterized in that: The recycling module includes a first waste box, a second waste box and a third waste box arranged on the mounting bracket; The first waste box is provided in the first storage area and is used to recycle the used TIP head; The second waste box is arranged in the second storage area and is used to recycle the used sampling container; The third waste box is arranged close to the detection module and is used for recovering the reagent bottles after detection.

4. The automated air sampling and detection device according to claim 3, characterized in that: The third waste box is provided with an inverting device; The inversion device comprises an inversion bracket and an inversion motor, wherein the inversion bracket is used to accommodate the reagent bottle, and the inversion motor drives the inversion bracket to rotate at least 90 degrees toward the direction of the third waste box; The second waste box is provided with a waste opening, and the waste opening is provided with a guiding slope extending toward the interior of the second waste box.

5. The automated air sampling and detection device according to claim 1, characterized in that: The collection module is an air sampling device, which includes a fan box, an air duct and a centrifugal part; The fan box is connected to the centrifugal part through the air duct. An air inlet is provided on the side of the centrifugal part. The air inlet is connected to the collection port. The bottom of the centrifugal part has a downwardly arranged collection hole, and the opening of the sampling container can abut against the collection hole.

6. The automated air sampling and detection device according to claim 5, characterized in that: The automated air sampling and detection device further comprises a protective cover device, wherein the protective cover device comprises a protective cover body and a cover body motor, and the cover body motor can rotatably drive the protective cover body to cover the collection port.

7. The automated air sampling and detection device according to any one of claims 1 to 6, characterized in that: The movable bracket comprises a horizontal movable mechanism and a mounting member connected to the horizontal movable mechanism; The mounting member is provided with a first horizontal moving mechanism and a second horizontal moving mechanism, the liquid transfer module is provided on the first horizontal moving mechanism, and the manipulator is provided on the second horizontal moving mechanism.

8. An automated detection method, characterized in that: An automated device for air sampling and detection comprising any one of claims 1 to 7, wherein the automated device for air sampling and detection further comprises at least a second reagent bottle and a third reagent bottle, wherein the second reagent bottle stores the second reagent and is provided with a second cover body for covering the second reagent bottle, and the third reagent bottle stores the third reagent and is provided with a third cover body for covering the third reagent bottle; The automated air sampling and detection device adopts the automated detection method, which includes the following steps: The manipulator moves to the storage device; The first reagent is pre-added in the sampling container, and the manipulator grabs the sampling container in the storage device and moves it to the pre-treatment position; The pretreatment device fixes the sampling container, and the manipulator loosens and removes the first cover from the sampling container; The pre-processing device moves the sampling container from the pre-processing position to the collection position; The collection module sucks air from the collection port and collects the objects to be detected in the air into the sampling container; The pre-processing device moves the sampling container collecting the object to be detected to the pre-processing position; The robot grabs the second reagent bottle in the storage device and moves it to the pretreatment position; The pretreatment device fixes the second reagent bottle, and the manipulator loosens and removes the second cover from the second reagent bottle; The manipulator grabs the third reagent bottle in the storage device and moves it to the pretreatment position; The pretreatment device fixes the third reagent bottle, and the manipulator loosens and removes the third cover from the third reagent bottle; The liquid transfer module moves the first reagent and the object to be detected in the sampling container to the second reagent in the second reagent bottle, and mixes them evenly; The liquid transfer module then moves the first reagent, the second reagent and the object to be detected mixed in the second reagent bottle to the third reagent in the third reagent bottle, thereby obtaining a final liquid to be detected; The manipulator covers the first cover body on the sampling container, covers the second cover body on the second reagent bottle, covers the third cover body on the third reagent bottle, and moves the third reagent bottle to the detection module for detection; The manipulator moves the second reagent bottle and the sampling container in the pretreatment position and the third reagent bottle in the detection module to the recovery module.

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