A Brillouin-OCTA-speckle multimodal elastic imaging system device

Abstract

The present invention discloses a Brillouin-OCTA-speckle multimodal elastography system device, comprising a Brillouin-OCTA sample scanning unit, a Brillouin scattering elastography system, an OCTA system, a speckle detection system, and a timing controller. The Brillouin scattering elastography system comprises a Brillouin scattering signal excitation unit and a Brillouin signal acquisition unit, the OCTA system comprises an OCTA signal excitation unit and an OCTA signal acquisition unit, and the speckle detection system comprises a speckle signal excitation unit and a speckle signal receiving unit. The Brillouin scattering elastography system and the OCTA system share the Brillouin-OCTA sample scanning unit. The present invention utilizes the advantages of the Brillouin scattering elastography system capable of high-resolution detection of body elastic modulus, the OCTA system's high-resolution structural imaging, and the speckle detection system's wide-field blood flow velocity detection to synchronously image vascular structure and elastic distribution in situ and quantify blood flow velocity, thereby providing a scientific basis and technical support for the early diagnosis of clinical vascular diseases.

Description

Technical Field

[0001] The present invention relates to the technical field of elastic imaging, and in particular to a Brillouin-OCTA-speckle multimodal elastic imaging system device. Background Art

[0002] The present invention is a measurement system device that mainly combines three technologies: Brillouin scattering elasticity imaging, optical coherence tomography, and speckle imaging to detect vascular elasticity, distribution, and blood flow velocity. The idea of ​​the invention is that blood vessels are the pipes through which the heart transports blood, and vascular diseases mainly refer to atherosclerosis, inflammatory vascular diseases, functional vascular diseases, and true tumor diseases of blood vessels. The consequences of pathological changes can be divided into three categories, mainly (1) the loss of elasticity of the blood vessel wall due to lesions; (2) lesions causing stenosis of the lumen; and (3) lesions causing damage to the vascular endothelium, which induces intravascular coagulation and thrombosis. In the early stages of vascular disease, the structure and elastic function of the blood vessels have already changed. Therefore, high-resolution and rapid detection of vascular structure, elasticity, and blood flow velocity is conducive to the early diagnosis and treatment of vascular diseases.

[0003] Brillouin scattering is an inelastic scattering process whose spectral characteristics are closely related to the properties of the medium (such as density, viscosity, and elastic modulus). Therefore, Brillouin scattering elastography can be used to measure the elastic modulus of vascular bodies. Optical coherence tomography angiography (OCTA) uses the principle of low-coherence interferometry to obtain tomographic maps of vascular distribution in depth. This technique can reconstruct vascular distribution images through scanning. Speckle imaging involves scattering particles on the tissue surface causing incident light to backscatter. Due to the different optical path lengths of the scattered light reaching the camera imaging plane, the scattered light forms random interference on the image plane, resulting in a spatially varying pattern of bright and dark particles. The movement of scattering particles (red blood cells) causes fluctuations in the intensity of the speckle pattern on the image plane. Detecting and analyzing these fluctuations can provide information about the velocity of the scattering particles. Therefore, the Brillouin-OCTA-speckle multimodal elastography system can not only simultaneously monitor vascular elasticity and distribution in situ, but also rapidly measure blood flow velocity over large areas, providing a scientific basis for the diagnosis and prevention of vascular diseases. Summary of the Invention

[0004] The purpose of the present invention is to solve the technical problems existing in the prior art and to provide a Brillouin-OCTA-speckle multimodal elastic imaging system device.

[0005] To achieve the above objectives, the present invention provides a technical solution: a Brillouin-OCTA-speckle multimodal elastography system, comprising a Brillouin-OCTA sample scanning unit, a Brillouin scattering elastography system, an OCTA system, a speckle detection system, and a timing controller. The Brillouin scattering elastography system and the OCTA system share the Brillouin-OCTA sample scanning unit. The Brillouin scattering elastography system includes a Brillouin scattering signal excitation unit and a Brillouin signal acquisition unit. The OCTA system includes an OCTA signal excitation unit and an OCTA signal acquisition unit. The speckle detection system includes a speckle signal excitation unit and a speckle signal receiving unit. The Brillouin-OCTA sample scanning unit comprises a dichroic mirror, a galvanometer mirror assembly, a first optical shutter, and a scanning lens.

[0006] The Brillouin-OCTA sample scanning unit is used to synchronously generate the OCTA signal and the Brillouin signal of the sample;

[0007] The Brillouin signal excitation unit is used to excite the Brillouin scattering signal;

[0008] The Brillouin signal acquisition unit is used to acquire Brillouin spectrum signals to generate vascular elasticity information;

[0009] The OCTA signal excitation unit is used to excite light scattering signals;

[0010] The OCTA signal acquisition unit is used to acquire OCTA signals to generate blood vessel distribution information;

[0011] The speckle signal excitation unit is used to excite wide-field speckle signals;

[0012] The speckle signal receiving unit is used to collect speckle signals to generate blood flow relative velocity information.

[0013] Preferably, the Brillouin scattering signal excitation unit is composed of a narrow-linewidth continuous laser, a first optical fiber isolator, an optical fiber delay line, an optical fiber circulator, a first collimator and a beam splitter; the Brillouin signal acquisition unit is composed of a fifth collimator, a Brillouin spectrometer and a first detector; in the Brillouin elastic imaging system, the narrow-linewidth continuous laser emits a light beam, which is split by the beam splitter after passing through the first optical fiber isolator, the optical fiber delay line, port 1 of the optical fiber circulator, port 2 of the optical fiber circulator and the first collimator; the reflected light is focused by the scanning lens onto the sample to be measured after passing through the dichroic mirror, the galvanometer group and the first optical shutter, and interacts with the sample to be measured to cause backward Brillouin scattering; the backward Brillouin scattering of the sample to be measured returns along the original path, is output from port 3 of the optical fiber circulator, is collimated by the fifth collimator, is incident on the Brillouin spectrometer for frequency demodulation, and is received by the first detector.

[0014] Preferably, the Brillouin scattering signal excitation unit adjusts the timing controller and the optical fiber delay line so that the excitation light of the Brillouin scattering elastic imaging system and the OCTA system reaches the dichroic mirror at the same time, thereby realizing synchronous scanning of samples, synchronous excitation and collection of vascular elasticity information and vascular distribution information by the OCTA system and the Brillouin system.

[0015] Preferably, the OCTA signal excitation unit is composed of an ultra-wideband light-emitting diode, a second fiber isolator, a 2*2 fiber coupler, and a second collimator; the OCTA signal acquisition unit is composed of a reference arm, a grating spectrometer, and a linear array CCD; in the OCTA system, the ultra-wideband light-emitting diode emits a light beam, which is collimated by the second fiber isolator, the 2*2 fiber coupler, and the second collimator, and then reflected by the dichroic mirror. The reflected light passes through the galvanometer group and the first optical shutter, and is focused by the scanning lens onto the sample to be measured. The backscattered light generated by the interaction with the sample returns along the original path, coheres with the light beam reflected by the reference arm, enters the grating spectrometer, and is collected and received by the linear array CCD.

[0016] Specifically, the reference arm includes a third collimator, an attenuator, and a first plane reflector, and the grating spectrometer includes a fourth collimator, a grating, and a plano-convex lens.

[0017] Preferably, the speckle signal excitation unit comprises a narrow-linewidth continuous laser, a first fiber isolator, a fiber delay line, a fiber circulator, a first collimator, a beam splitter, a second optical shutter, a second plane reflector, and a beam expander; the speckle signal receiving unit comprises a scanning lens, a flip reflector, and a second detector. In the speckle detection system, the narrow-linewidth continuous laser emits a light beam, which passes through the first fiber isolator, the fiber delay line, port 1 and port 2 of the fiber circulator, and the first collimator, and is then split by the beam splitter. The light beam transmitted through the beam splitter passes through the second optical shutter, the second plane reflector, and the beam expander, and is incident on a sample to be measured. A scattered signal generated by the interaction with the sample to be measured passes through the scanning lens, is reflected by the flip reflector to the second detector for detection.

[0018] Preferably, the speckle signal excitation unit expands the spot size through the beam expander to excite a wide-field speckle signal at the sample.

[0019] Preferably, the speckle signal excitation unit and the Brillouin-OCTA sample scanning unit are used alternately through the first optical shutter and the second optical shutter, thereby achieving the alternate use of the speckle signal excitation unit and the Brillouin-OCTA sample scanning unit.

[0020] Preferably, the Brillouin-OCTA sample scanning unit realizes scanning on the XY plane of the sample through a galvanometer group, and adjusts the sample height to realize scanning on the XY plane at different depths of the sample.

[0021] Beneficial effects of the present invention:

[0022] The present invention utilizes the advantages of the Brillouin scattering elastography system's ability to detect body elastic modulus with high resolution, the OCTA system's high-resolution structural imaging, and the speckle detection system's wide-field blood flow velocity detection to simultaneously image vascular structure and elastic distribution in situ and quantify blood flow velocity, thereby providing a scientific basis and technical support for the early diagnosis of clinical vascular diseases. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The drawings described herein are used to provide further understanding of the present invention and constitute a part of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute improper limitations on the present invention.

[0024] Figure 1 The invention relates to a Brillouin-OCTA-speckle multimodal vascular elasticity and blood flow velocity detection device.

[0025] Figure annotation:

[0026] 1- Narrow linewidth continuous laser 2- First fiber isolator 3- Fiber delay line 4- Fiber circulator 5- First collimator 6- Beam splitter 7- Ultra-wideband light-emitting diode 8- Second fiber isolator 9- 2*2 fiber coupler 10- Second collimator 11- Dichroic mirror 12- Galvanometer assembly 13- First optical shutter 14- Scan lens 15- Third collimator 16- Attenuator 17- First plane mirror 18- Fourth collimator 19- Grating 20- Plano-convex lens 21- Linear array CCD 22- Fifth collimator 23- Brillouin spectrometer 24- First detector 25- Second optical shutter 26- Second plane mirror 27- Beam expander 28- Flip mirror 29- Second detector 30- Sample 31- Timing controller 32- Computer DETAILED DESCRIPTION

[0027] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it should not be understood as a limitation on the scope of protection of the present invention.

[0028] In the description of the present invention, "several" means one or more, "many" means more than two, "greater than," "less than," and "exceed" are understood to exclude the number itself, while "above," "below," and "within" are understood to include the number itself. The use of "first" and "second" in the description is solely for the purpose of distinguishing technical features and should not be construed as indicating or implying relative importance, implicitly specifying the number of the indicated technical features, or implicitly specifying the order of the indicated technical features.

[0029] Reference Figure 1 A preferred embodiment of the present invention provides a Brillouin-OCTA-speckle multimodal elastography system, comprising a Brillouin-OCTA sample scanning unit, a Brillouin scattering elastography system, an OCTA system, a speckle detection system, and a timing controller 31. The Brillouin scattering elastography system, the OCTA system, and the speckle detection system share the Brillouin-OCTA sample scanning unit. The Brillouin scattering elastography system includes a Brillouin scattering signal excitation unit and a Brillouin signal acquisition unit. The OCTA system includes an OCTA signal excitation unit and an OCTA signal acquisition unit. The speckle detection system includes a speckle signal excitation unit and a speckle signal receiving unit. The Brillouin-OCTA sample scanning unit comprises a dichroic mirror 11, a galvanometer mirror assembly 12, a first optical shutter 13, and a scanning lens 14.

[0030] The Brillouin-OCTA sample scanning unit is used to synchronously generate an OCTA signal and a Brillouin signal of the sample 30;

[0031] The Brillouin signal excitation unit is used to excite the Brillouin scattering signal;

[0032] The Brillouin signal acquisition unit is used to acquire Brillouin spectrum signals to generate vascular elasticity information;

[0033] The OCTA signal excitation unit is used to excite light scattering signals;

[0034] The OCTA signal acquisition unit is used to acquire OCTA signals to generate blood vessel distribution information;

[0035] The speckle signal excitation unit is used to excite wide-field speckle signals;

[0036] The speckle signal receiving unit is used to collect speckle signals to generate blood flow relative velocity information.

[0037] As a preferred embodiment of the present invention, it may also have the following additional technical features:

[0038] In this embodiment, the Brillouin scattering signal excitation unit is composed of a narrow linewidth continuous laser 1, a first optical fiber isolator 2, an optical fiber delay line 3, an optical fiber circulator 4, a first collimator 5 and a beam splitter 6; the Brillouin signal acquisition unit is composed of a fifth collimator 22, a Brillouin spectrometer 23 and a first detector 24; in the Brillouin elastic imaging system, the narrow linewidth continuous laser 1 emits a light beam, which passes through the first optical fiber isolator 2, the optical fiber delay line 3, the No. 1 port of the optical fiber circulator 4, the No. 2 port of the optical fiber circulator 4, the No. 3 port of the optical fiber circulator 4, the No. 4 port of the optical fiber circulator 4, the No. 5 port of the optical fiber circulator 4, the No. 6 port of the optical fiber circulator 4, the No. 7 port of the optical fiber circulator 4, the No. 8 port of the optical fiber circulator 4, the No. 9 port of the optical fiber circulator 4, the No. 1 port of the optical fiber circulator 4, the No. 2 port of the optical fiber circulator 4, the No. 3 port of the optical fiber circulator 4, the No. 4 port of the optical fiber circulator 4, the No. 5 port of the optical fiber circulator 4, the No. 6 port of the optical fiber circulator 4, the No. 7 port of the optical fiber circulator 4, the No. 8 port of the optical fiber circulator 4, the No. 8 port of the optical fiber circulator 4, the No. 9 port of the optical fiber circulator 4, the No. 1 port of the optical fiber circulator 4, the No. 2 port of the optical fiber circulator 4, the No. 3 port of the optical fiber circulator 4, the No. 3 The reflected light passes through port No. 2 of the optical fiber circulator 4 and the first collimator 5 and is then split by the beam splitter 6. The reflected light passes through the dichroic mirror 11, the galvanometer group 12 and the first optical shutter 13, and is focused by the scanning lens 14 onto the sample 30 to be measured. The reflected light interacts with the sample 30 to be measured and causes backward Brillouin scattering. The backward Brillouin scattering of the sample 30 to be measured returns along the original path, is output from port No. 3 of the optical fiber circulator 4, is collimated by the fifth collimator 22, is incident on the Brillouin spectrometer 23 for frequency demodulation, and is received by the first detector 24.

[0039] In this embodiment, the Brillouin scattering signal excitation unit adjusts the timing controller 31 and the optical fiber delay line 3 so that the excitation light of the Brillouin scattering elastic imaging system and the OCTA system reaches the dichroic mirror 11 at the same time, thereby realizing that the OCTA system and the Brillouin system synchronously scan the sample 30, synchronously excite and collect vascular elasticity information and vascular distribution information.

[0040] In this embodiment, the OCTA signal excitation unit is composed of an ultra-wideband light-emitting diode 7, a second fiber isolator 8, a 2*2 fiber coupler 9, and a second collimator 10; the OCTA signal acquisition unit is composed of a reference arm, a grating spectrometer, and a linear array CCD21; in the OCTA system, the ultra-wideband light-emitting diode 7 emits a light beam, which is collimated by the second fiber isolator 8, the 2*2 fiber coupler 9, and the second collimator 10, and then reflected by the dichroic mirror 11. The reflected light passes through the galvanometer group 12 and the first optical shutter 13, and is focused by the scanning lens 14 onto the sample 30 to be measured. The backscattered light generated by the interaction with the sample 30 returns along the original path, coheres with the light beam reflected by the reference arm, enters the grating spectrometer 19, and is collected and received by the linear array CCD21.

[0041] Specifically, the timing controller 31 is connected to the narrow linewidth continuous laser 1 and the ultra-wideband light emitting diode 7 .

[0042] Specifically, the reference arm includes a third collimator 15 , an attenuator 16 , and a first plane reflector 17 , and the grating spectrometer includes a fourth collimator 18 , a grating 19 , and a plano-convex lens 20 .

[0043] In this embodiment, the speckle signal excitation unit comprises a narrow-linewidth continuous laser 1, a first fiber isolator 2, a fiber delay line 3, a fiber circulator 4, a first collimator 5, a beam splitter 6, a second optical shutter 25, a second plane mirror 26, and a beam expander 27. The speckle signal receiving unit comprises a scanning lens 14, a flip mirror 28, and a second detector 29. In the speckle detection system, the narrow-linewidth continuous laser 1 emits a light beam, which passes through the first fiber isolator 2, the fiber delay line 3, port 1 and port 2 of the fiber circulator 4, and the first collimator 5, and is then split by the beam splitter 6. The light beam transmitted through the beam splitter 6 passes through the second optical shutter 25, the second plane mirror 26, and the beam expander 27, and is incident on a sample 30 to be measured. A scattered signal generated by the interaction with the sample 30 passes through the scanning lens 14, is reflected by the flip mirror 28, and is then detected by the second detector 29.

[0044] Specifically, the speckle signal receiving unit reflects the speckle signal and collects the signal when the speckle detection system detects the sample 30 by flipping the reflector 28. When the speckle detection system is not working, the reflector 28 is flipped again to transmit the detection light of the Brillouin-OCTA sample scanning unit.

[0045] In this embodiment, the speckle signal excitation unit expands the spot size through the beam expander 27 to excite a wide-field speckle signal at the sample 30 .

[0046] In this embodiment, the speckle signal excitation unit and the Brillouin-OCTA sample scanning unit are used alternately through the first optical shutter 13 and the second optical shutter 25 , thereby achieving the alternate use of the speckle signal excitation unit and the Brillouin-OCTA sample scanning unit.

[0047] In this embodiment, the Brillouin-OCTA sample scanning unit adjusts the height of the sample 30 through the galvanometer group 11 and scans the sample 30 at different depths on the XY plane.

[0048] The present invention operates as follows: the speckle detection system, the Brillouin elastic imaging system, and the OCTA system alternately use the first optical shutter 13 and the second optical shutter 25, in combination with the flip mirror 28, to achieve alternate use of the speckle signal excitation unit and the Brillouin-OCTA sample scanning unit. During speckle detection system imaging, the first optical shutter 13 is closed, the second optical shutter 25 is opened, and the flip mirror 28 is rotated into the optical path. The light beam transmitted by the beam splitter 6 is expanded by the beam expander 27, and the light spot is enlarged. The light beam is incident on the sample 30, enabling wide-field scanning of the sample 30 and further detecting the flow velocity of blood vessels within the wide-field range. During operation of the Brillouin optical elastic imaging system and the OCTA system, the first optical shutter 13 is opened, the second optical shutter 25 is closed, and the flip mirror 28 is rotated out of the optical path. After passing through the Brillouin-OCTA sample scanning unit, the backscattered signal is received by the Brillouin signal acquisition unit and the OCTA signal acquisition unit, respectively.

[0049] Specifically, when testing biological tissues, there are three main steps:

[0050] In the first step, the speckle detection system performs a wide-field scan on the sample 30 to detect the flow velocity of the blood vessels within the wide-field range. The details are as follows:

[0051] ① When the speckle detection system performs wide-field scanning, the second optical shutter 25 is opened to turn on the speckle signal excitation unit; the flip mirror 28 is turned into the optical path to turn on the speckle signal receiving unit.

[0052] ② Speckle signal excitation unit: a beam of light split by the beam splitter 6 passes through the second optical shutter 25, is reflected by the second plane reflector 26, and then enters the beam expander 27 for beam expansion. It hits the sample 30 in the form of a wide-field light spot and interacts with the sample.

[0053] ③ Speckle signal receiving unit: the speckle signal generated at the sample 30 passes through the scanning lens 14, is reflected by the flip mirror 28 and enters the detector 29, collects the speckle signal, and uses the computer 32 to process the collected signal.

[0054] The second step is to switch the system. Turn off the speckle detection system and turn on the Brillouin scattering elastography system and OCTA system. The details are as follows:

[0055] The second optical shutter 25 is closed to block the light beam from entering the speckle signal excitation unit. The first optical shutter 13 is opened, and the flip mirror 28 is rotated away from the light path, so that the light beam after the galvanometer group 12 can pass through the first optical shutter 13 and enter the scanning lens 14.

[0056] In the third step, the Brillouin scattering elastography system and the OCTA system simultaneously examine the sample. The Brillouin scattering elastography system measures the elastic modulus of the vascular volume, while the OCTA system scans and reconstructs the vascular distribution image. To achieve synchronous detection of the two systems, the Brillouin optical elastography system and the OCTA system share the Brillouin-OCTA sample scanning unit. Specific details are as follows:

[0057] ① The shared Brillouin-OCTA sample scanning unit mainly includes a dichroic mirror 11, a galvanometer mirror group 12, a first optical shutter 13 and a scanning lens 14. The dichroic mirror 11 transmits the excitation light from the Brillouin scattering elastic imaging system into the Brillouin-OCTA sample scanning unit, and reflects the excitation light from the OCTA system into the Brillouin-OCTA sample scanning unit. The light beam entering the Brillouin-OCTA sample scanning unit is finally focused on the sample 30, interacting with the sample 30 to generate a scattering signal;

[0058] ② In the Brillouin scattering signal excitation unit, a narrow-linewidth continuous laser 1 releases 780nm excitation light, which enters port 1 of the circulator 4 after passing through the first fiber isolator 2 and the fiber delay line 3. The spatial light output from port 2 of the circulator 4 is collimated by the first collimator 5 and split by the beam splitter 6. Part of the light enters the Brillouin-OCTA sample scanning unit.

[0059] ③ In the OCTA signal excitation unit of the OCTA system, the 1310nm excitation light emitted by the ultra-wideband light-emitting diode 7 passes through the second fiber isolator 8 and the 2*2 fiber coupler 9, is collimated by the second collimator 10, and enters the Brillouin-OCTA sample scanning unit;

[0060] ④ Adjust the timing controller 31 and the optical fiber delay line 3 to control the timing of the two lasers to ensure that the light beams output by the two lasers reach the dichroic mirror 11 at the same time;

[0061] ⑤ The signal light generated at sample 30 returns along the original optical path from the Brillouin-OCTA sample scanning unit and is collected by the signal receiving unit. The Brillouin scattering elastography system and the OCTA system have independent signal receiving units. In the Brillouin scattering signal receiving unit, the backscattered signal generated at sample 30 returns along the original optical path, passes through the fiber circulator 4, is collimated by the fifth collimator 22, enters the Brillouin spectrometer 23, and is spectrally detected by the first detector 24. The collected spectral signal is processed by the computer 32.

[0062] In the OCTA signal acquisition unit, the backscattered signal returning along the original path of the Brillouin-OCTA sample scanning unit is reflected by dichroic mirror 11, passes through second collimator 10, enters 2x2 fiber coupler 9, and is output by fourth collimator 18. The output scattered signal is split by grating spectrometer 19 and focused by plano-convex lens 20 onto linear array CCD 21 for signal acquisition. The collected signal is then processed by computer 32.

[0063] The present invention utilizes the advantages of the Brillouin scattering elastography system's ability to detect body elastic modulus with high resolution, the OCTA system's high-resolution structural imaging, and the speckle detection system's wide-field blood flow velocity detection to simultaneously image vascular structure and elastic distribution in situ and quantify blood flow velocity, thereby providing a scientific basis and technical support for the early diagnosis of clinical vascular diseases.

[0064] Under the premise that no conflict occurs, those skilled in the art may freely combine and superimpose the above-mentioned additional technical features.

[0065] The above descriptions are only preferred embodiments of the present invention. Any technical solution that achieves the purpose of the present invention by substantially the same means shall fall within the scope of protection of the present invention.

Claims

1. A Brillouin-OCTA-speckle multimodal elastic imaging system, characterized by: The system comprises a Brillouin-OCTA sample scanning unit, a Brillouin scattering elastic imaging system, an OCTA system, a speckle detection system, and a timing controller. The Brillouin scattering elastic imaging system and the OCTA system share the Brillouin-OCTA sample scanning unit. The Brillouin scattering elastic imaging system comprises a Brillouin scattering signal excitation unit and a Brillouin scattering signal acquisition unit. The OCTA system comprises an OCTA signal excitation unit and an OCTA signal acquisition unit. The speckle detection system comprises a speckle signal excitation unit and a speckle signal receiving unit. The Brillouin-OCTA sample scanning unit comprises a dichroic mirror, a galvanometer mirror group, a first optical shutter, and a scanning lens. The Brillouin-OCTA sample scanning unit is used to synchronously generate an OCTA signal and a Brillouin scattering signal of the sample; The Brillouin scattering signal excitation unit is used to excite the Brillouin scattering signal; The Brillouin scattering signal acquisition unit is used to acquire Brillouin scattering signals to generate vascular elasticity information; The OCTA signal excitation unit is used to excite light scattering signals; The OCTA signal acquisition unit is used to acquire OCTA signals to generate blood vessel distribution information; The speckle signal excitation unit is used to excite wide-field speckle signals; The speckle signal receiving unit is used to collect speckle signals to generate blood flow relative velocity information; The Brillouin scattering signal excitation unit is composed of a narrow-linewidth continuous laser, a first optical fiber isolator, an optical fiber delay line, an optical fiber circulator, a first collimator and a beam splitter; the Brillouin scattering signal acquisition unit is composed of a fifth collimator, a Brillouin spectrometer and a first detector; in the Brillouin scattering elastic imaging system, the narrow-linewidth continuous laser emits a light beam, which is split by the beam splitter after passing through the first optical fiber isolator, the optical fiber delay line, port 1 of the optical fiber circulator, port 2 of the optical fiber circulator and the first collimator; the reflected light is focused by the scanning lens onto the sample to be measured after passing through the dichroic mirror, the galvanometer group and the first optical shutter, and interacts with the sample to be measured to generate backward Brillouin scattering; the backward Brillouin scattering of the sample to be measured returns along the original path, is output from port 3 of the optical fiber circulator, is collimated by the fifth collimator, is incident on the Brillouin spectrometer for frequency discrimination, and is received by the first detector; The Brillouin scattering signal excitation unit adjusts the timing controller and the optical fiber delay line so that the excitation light of the Brillouin scattering elastography system and the OCTA system reaches the dichroic mirror at the same time, thereby realizing that the OCTA system and the Brillouin scattering elastography system synchronously scan the sample, synchronously excite and collect vascular elasticity information and vascular distribution information; The speckle signal excitation unit comprises a narrow-linewidth continuous laser, a first fiber isolator, a fiber delay line, a fiber circulator, a first collimator, a beam splitter, a second optical shutter, a second plane reflector, and a beam expander. The speckle signal receiving unit comprises a scanning lens, a flip reflector, and a second detector. In the speckle detection system, the narrow-linewidth continuous laser emits a light beam, which passes through the first fiber isolator, the fiber delay line, port 1 and port 2 of the fiber circulator, and the first collimator, and is then split by the beam splitter. The light beam transmitted through the beam splitter passes through the second optical shutter, the second plane reflector, and the beam expander, and is incident on a sample to be measured. The scattered signal generated by the interaction with the sample passes through the scanning lens, is reflected by the flip reflector, and is then detected by the second detector.

2. The Brillouin-OCTA-speckle multimodal elastography system according to claim 1, characterized in that: The OCTA signal excitation unit consists of an ultra-wideband light-emitting diode, a second fiber isolator, a 2*2 fiber coupler, and a second collimator; the OCTA signal acquisition unit consists of a reference arm, a grating spectrometer, and a linear array CCD. In the OCTA system, the ultra-wideband light-emitting diode emits a light beam, which is collimated by the second fiber isolator, the 2*2 fiber coupler, and the second collimator, and then reflected by the dichroic mirror. The reflected light passes through the galvanometer group and the first optical shutter, and is focused by the scanning lens onto the sample to be measured. The backscattered light generated by the interaction with the sample returns along the original path, coheres with the light beam reflected by the reference arm, enters the grating spectrometer, and is collected and received by the linear array CCD.

3. The Brillouin-OCTA-speckle multimodal elastic imaging system according to claim 1, characterized in that: The speckle signal excitation unit expands the spot size through the beam expander to excite a wide-field speckle signal at the sample.

4. The Brillouin-OCTA-speckle multimodal elastic imaging system according to claim 1, characterized in that: The speckle signal excitation unit and the Brillouin-OCTA sample scanning unit are used alternately through the first optical shutter and the second optical shutter, thereby achieving the alternate use of the speckle signal excitation unit and the Brillouin-OCTA sample scanning unit.

5. The Brillouin-OCTA-speckle multimodal elastic imaging system according to claim 1, characterized in that: The Brillouin-OCTA sample scanning unit realizes scanning on the XY plane of the sample through a galvanometer group, and adjusts the sample height to realize scanning on the XY plane at different depths of the sample.

Images