A control system for PCR detection

Abstract

The present application discloses a control system for PCR detection, which relates to the field of measurement technology, and includes a temperature control module and a fluorescence detection probe control module. The temperature control module is used for temperature control of PCR thermal cycle and signal processing of fluorescence detection, and the fluorescence detection probe control module is used for realizing position control of the detection probe and fluorescence signal detection. It has the following advantages: using Peltier as a heat pump element of the thermal cycle of PCR detection, the thermal cycle metal block can be quickly heated and cooled, and the Peltier is controlled by DC voltage regulation, which can greatly reduce the current ripple of the Peltier, improve the temperature control accuracy and thus improve the detection accuracy, and add an auxiliary heating function, which can compensate for the temperature and reduce the temperature difference of the thermal cycle tank.

Description

Technical Field

[0001] The invention relates to a control system for PCR detection, belonging to the technical field of measurement. Background Art

[0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify specific DNA fragments. Real-time fluorescence quantitative PCR technology refers to adding fluorescent groups to the PCR reaction system, using the accumulation of fluorescent signals to monitor the entire PCR process in real time, and finally using the standard curve to quantitatively analyze unknown templates.

[0003] PCR detection, also known as fluorescent quantitative PCR detector, refers to an instrument that uses fluorescent dyes or fluorescently labeled specific probes to mark and track PCR products and monitor the reaction process in real time. Combined with the corresponding software, it can perform qualitative and quantitative analysis on the products, calculate the initial concentration of the sample template to be tested, add fluorescent groups to the PCR reaction system, and use the accumulation of fluorescent signals to monitor the entire PCR process in real time.

[0004] Existing PCR detectors have the following disadvantages:

[0005] 1. Using thin film heater as thermal cycle element cannot quickly raise or lower the temperature of thermal cycle metal block;

[0006] 2. The detector that uses Peltier as the heat pump element of the thermal cycle of PCR detection uses PWM control to control the Peltier, which reduces the detection accuracy;

[0007] 3. There is no auxiliary heating function, resulting in poor consistency of test results;

[0008] 4. The excitation light source adopts a single light source, and the light intensity of the light source is low. Summary of the invention

[0009] The technical problem to be solved by the present invention is to provide a control system for PCR detection in view of the above shortcomings. Peltier is used as a heat pump element for the thermal cycle of PCR detection, which can quickly raise and lower the temperature of the thermal cycle metal block. The Peltier is controlled by DC voltage regulation, which can greatly reduce the current ripple of the Peltier, improve the temperature control accuracy and thus improve the detection accuracy. An auxiliary heating function is added, which can compensate for the temperature and reduce the temperature difference of the thermal cycle tank.

[0010] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0011] A control system for PCR detection, comprising a temperature control module and a fluorescence detection probe control module, wherein the temperature control module is used for temperature control of PCR thermal cycle and signal processing of fluorescence detection, and the fluorescence detection probe control module is used for position control of the detection probe and fluorescence signal detection;

[0012] The temperature control module includes a 1# processor unit, a 1# auxiliary heating compensation unit, a 2# auxiliary heating compensation unit, a 1# semiconductor refrigeration unit, a 2# semiconductor refrigeration unit, a power supply and a cooling fan control unit, the processor unit is connected to the 1# auxiliary heating compensation unit, the 2# auxiliary heating compensation unit, the 1# semiconductor refrigeration unit, the 2# semiconductor refrigeration unit, the 1# power supply and the cooling fan control unit, and the 1# processor unit is also connected to an Android display and a thermal printer through an interface;

[0013] The fluorescence detection probe control module includes a 2# processor unit, a fluorescence detection unit, an excitation light source driving unit and a stepper motor driving unit, and the 2# processor unit is connected to the fluorescence detection unit, the excitation light source driving unit and the stepper motor driving unit.

[0014] Furthermore, the processor unit #1 includes a chip U29, and the model of the chip U29 is HC32F072PATA;

[0015] The 2# processor unit includes a chip U43, and the model of the chip U43 is HC32F030J8TA.

[0016] Further, the 1# auxiliary heating compensation unit includes a chip U1, the model of the chip U1 is TM7707, the 1st pin of the chip U1 is connected to the 53rd pin of the chip U29, the 2nd pin of the chip U1 is connected to the 52nd pin of the chip U29, the 4th pin of the chip U1 is connected to the 58th pin of the chip U29, the 5th pin of the chip U1 is connected to the 51st pin of the chip U29, the 12th pin of the chip U1 is connected to the 59th pin of the chip U29, the 13th pin of the chip U1 is connected to the 60th pin of the chip U29, the 14th pin of the chip U1 is connected to the 57th pin of the chip U29, and the 6th pin of the chip U1 is connected to the interface P Pin 2 of chip U1 is connected to one end of resistor RJ1, and the other end of resistor RJ1 is grounded. Pin 7 of chip U1 is connected to one end of resistor R1 and pin 1 of interface P2. Interface P2 is used for external temperature sensor. Pin 9 of chip U1 is connected to VOUT pin of chip U2, one end of capacitor C3 and the other end of resistor R1, and the other end of capacitor C3 is grounded. VIN pin and EN pin of chip U2 are connected to one end of capacitor C2, one end of capacitor C1 and pin 15 of chip U1. The other end of capacitor C2 and the other end of capacitor C1 are grounded and connected to +3V3 power supply. The model of chip U2 is XC6209F122MR.

[0017] The 1# auxiliary heating compensation unit also includes a MOS tube Q9, a G pole of the MOS tube Q9 is connected to one end of a resistor R62, the other end of the resistor R62 is grounded, a D pole of the MOS tube Q9 is connected to pin 2 of the interface P15, a pin 1 of the interface P15 is connected to one end of a capacitor C80 and connected to a VIN power supply, and the other end of the capacitor C80 and the S pole of the MOS tube Q9 are grounded.

[0018] Further, the 1# semiconductor refrigeration unit includes a chip U6, the model of the chip U6 is MP2905, the 1st pin of the chip U6 is connected to one end of the resistor R5 and one end of the diode D2, the other end of the resistor R5 is connected to the 1st and 4th pins of the operational amplifier U5, the 3rd pin of the operational amplifier U5 is connected to one end of the capacitor C9 and one end of the resistor R4, the other end of the capacitor C9 is grounded, the other end of the resistor R4 is connected to one end of the resistor R3 and one end of the capacitor C8, the other end of the capacitor C8 is grounded, the other end of the resistor R3 is connected to the 44th pin of the chip U29, and the other end of the diode D2 is connected to the operational amplifier Pin 1 of U7 is connected to one end of capacitor C25, pin 3 of op amp U7 is connected to one end of capacitor C22, one end of resistor R9 and one end of resistor R8, pin 2 of op amp U7 and the other end of resistor R9 are connected to +3V3 power supply, the other end of resistor R8 and the other end of capacitor C22 are grounded, pin 4 of op amp U7 is connected to one end of resistor R14 and one end of resistor R15, the other end of resistor R14 is connected to pin 1 of chip U6, the other end of resistor R15 is connected to the other end of capacitor C25, pin 3 of chip U6 is connected to one end of capacitor C20 and one end of diode D3, the other end of capacitor C20 is grounded;

[0019] Pin 7 of the chip U6 is connected to one end of capacitor C15, the D pole of MOS tube Q2, the S pole of MOS tube Q1, one end of capacitor C18, one end of capacitor C19 and one end of inductor L1. The other end of capacitor C15 is connected to pin 6 of chip U6 and the other end of diode D3. The G pole of MOS tube Q2 is connected to pin 5 of chip U6. The S pole of MOS tube Q2 is grounded. The other end of capacitor C18 is connected to one end of resistor R10. The other end of resistor R10 is grounded. 9 The other end is connected to one end of a resistor R12, the other end of the resistor R12 is connected to one end of a resistor R13 and pin 1 of the chip U6, the other end of the resistor R13 is grounded, the other end of the inductor L1 is connected to one end of a resistor R11, one end of a capacitor C23 and one end of a capacitor C21, and connected to the PW_TEC1 power supply, the other end of the capacitor C23 and the other end of the capacitor C21 are grounded, the other end of the resistor R11 is connected to one end of a capacitor C24 and pin 1 of the chip U6, the other end of the capacitor C24 is grounded;

[0020] Pin 8 of the chip U6 is connected to one end of a resistor R7 and one end of a diode D1, the other end of the resistor R7 and the other end of the diode D1 are connected to the G pole of the MOS tube Q1, pin 9 of the chip U6 is connected to one end of a capacitor C16 and connected to the VIN power supply, the other end of the capacitor C16 is grounded, pin 10 of the chip U6 is connected to one end of a capacitor C13 and one end of a resistor R6, the other end of the capacitor C13 and the other end of the resistor R6 are connected to the D pole of the MOS tube Q1, one end of a capacitor C10, one end of a capacitor C12 and one end of a capacitor C11, and connected to the VIN power supply, the other end of the capacitor C10, the other end of the capacitor C12 and the other end of the capacitor C11 are grounded.

[0021] Furthermore, the 1# semiconductor refrigeration unit also includes a chip U8 and a chip U10, both of which are WSP4620. Pin 2 of chip U8 is connected to one end of resistor R19 and pin 3 of optocoupler U9, the other end of resistor R19 is grounded, pin 2 of optocoupler U9 is connected to one end of resistor R16, the other end of resistor R16 is grounded, pin 4 of optocoupler U9 is connected to +5V0 power supply, and pin 1 of optocoupler U9 is connected to pin 4 of chip U29. 5 pin, the 4th pin of chip U8 is connected to the 4th pin of optocoupler U12 and one end of resistor R21, the other end of resistor R21 is connected to PW_TEC1 power supply, the 2nd pin of optocoupler U12 is connected to one end of resistor R22, the other end of resistor R22 is grounded, the 1st pin of optocoupler U12 is connected to the 46th pin of chip U29, the 3rd pin of optocoupler U12 is connected to the 3rd pin of chip U15, the 2nd pin of chip U10 is connected to one end of resistor R18 and the 3rd pin of optocoupler U11, the resistor R The other end of 18 is grounded, the 2nd foot of the optocoupler U11 is connected to one end of the resistor R17, the other end of the resistor R17 is grounded, the 4th foot of the optocoupler U11 is connected to the +5V0 power supply, the 1st foot of the optocoupler U11 is connected to the 47th foot of the chip U29, the 4th foot of the chip U10 is connected to the 4th foot of the optocoupler U14 and one end of the resistor R20, the other end of the resistor R20 is connected to the PW_TEC1 power supply, the 2nd foot of the optocoupler U14 is connected to one end of the resistor R23, the other end of the resistor R23 is grounded Pin 1 of the optocoupler U14 is connected to pin 49 of the chip U29, pin 3 of the optocoupler U14 is connected to pin 3 of the chip U15, the model of the chip U15 is B0509S-1W, pin 2 of the chip U15 is connected to one end of the capacitor C27 and to the +5V0 power supply, the other end of the capacitor C27 is grounded, pin 4 of the chip U15 is connected to one end of the capacitor C28 and to the PW_TEC1 power supply, the other end of the capacitor C28 is connected to pin 3 of the chip U15.

[0022] Furthermore, the 1# semiconductor refrigeration unit also includes a chip U13, the model of the chip U13 is TM7707, the 1st pin of the chip U13 is connected to the 53rd pin of the chip U29, the 2nd pin of the chip U13 is connected to the 52nd pin of the chip U29, the 4th pin of the chip U13 is connected to the 54th pin of the chip U29, the 5th pin of the chip U13 is connected to the 51st pin of the chip U29, the 12th pin of the chip U13 is connected to the 55th pin of the chip U29, the 13th pin of the chip U13 is connected to the 56th pin of the chip U29, the 14th pin of the chip U13 is connected to the 57th pin of the chip U29, and the 6th pin of the chip U13 is connected to the interface P5. Pin 2 and one end of resistor RJ3, the other end of resistor RJ3 is grounded, pin 7 of chip U13 is connected to one end of resistor R24 ​​and pin 1 of interface P5, interface P5 is used for an external temperature sensor, pin 9 of chip U13 is connected to the VOUT pin of chip U16, one end of capacitor C31 and the other end of resistor R24, the other end of capacitor C31 is grounded, the VIN pin and EN pin of chip U16 are connected to one end of capacitor C30, one end of capacitor C29 and pin 15 of chip U13, and are connected to a +3V3 power supply, the other end of capacitor C29 and the other end of capacitor C30 are grounded, and the model of chip U16 is XC6209F122MR.

[0023] Further, the cooling fan control unit includes a chip U30 and a chip U32, the models of the chip U30 and the chip U32 are TC1047AVNBTR, the pin 1 of the chip U30 is connected to one end of the capacitor C58 and connected to the +3V3 power supply, the other end of the capacitor C58 is grounded, the pin 3 of the chip U30 is connected to the pin 35 of the chip U29, the pin 1 of the chip U32 is connected to one end of the capacitor C62 and connected to the +3V3 power supply, the other end of the capacitor C62 is grounded, and the pin 3 of the chip U32 is connected to the pin 37 of the chip U29;

[0024] The cooling fan control unit also includes a MOS tube Q5 and a MOS tube Q6, wherein the G pole of the MOS tube Q5 is connected to one end of a resistor R49 and pin 29 of a chip U29, the S pole of the MOS tube Q5 and the other end of the resistor R49 are grounded, the D pole of the MOS tube Q5 is connected to pin 3 of an interface P10, pin 2 of the interface P10 is connected to a VIN power supply, and pin 4 of the interface P10 is connected to pin 29 of the chip U29; the G pole of the MOS tube Q6 is connected to one end of a resistor R50 and pin 91 of the chip U29, the S pole of the MOS tube Q6 and the other end of the resistor R50 are grounded, the D pole of the MOS tube Q6 is connected to pin 3 of an interface P11, pin 2 of the interface P11 is connected to a VIN power supply, and pin 4 of the interface P11 is connected to pin 91 of the chip U29.

[0025] Furthermore, the fluorescence detection unit includes a chip U33, a chip U34 and a chip U36. The models of the chips U33, U34 and U36 are all MCP3421. Pin 3 of the chip U33 is connected to one end of a resistor R51 and pins 43 and 19 of the chip U43. The other end of the resistor R51 is connected to a +3V3 power supply. Pin 4 of the chip U33 is connected to one end of a resistor R52 and pins 42 and 20 of the chip U43. The other end of the resistor R52 is connected to a +3V3 power supply. Pin 5 of the chip U33 is connected to one end of a capacitor C66 and connected to a +3V3 power supply. The other end of the capacitor C66 is grounded. Pin 1 of 33 is connected to pin 1 of op amp U2A and one end of photocell D11, pin 2 of op amp U2A is connected to one end of capacitor C67, one end of resistor R55 and the other end of photocell D11, the other end of capacitor C67 and the other end of resistor R55 are connected to pin 4 of op amp U2A and pin 6 of op amp U2B, pin 7 of op amp U2B is connected to one end of resistor R54 and one end of resistor R56, the other end of resistor R56 is connected to VREF_1V25 power supply, the other end of resistor R54 is connected to pin 8 of op amp U2B and pin 6 of chip U33, the models of op amp U2A and op amp U2B are LMC6482AIM;

[0026] The 3rd pin of the chip U34 is connected to the 43rd pin and the 19th pin of the chip U43, the 4th pin of the chip U34 is connected to one end of the resistor R57 and the 41st pin and the 21st pin of the chip U43, the other end of the resistor R57 is connected to the +3V3 power supply, the 5th pin of the chip U34 is connected to one end of the capacitor C68 and the +3V3 power supply, the other end of the capacitor C68 is grounded, the 1st pin of the chip U34 is connected to the 1st pin of the operational amplifier U5A and one end of the photocell D12, the 2nd pin of the operational amplifier U5A is connected to the capacitor C70, The end, one end of the resistor R60 and the other end of the photocell D12, the other end of the capacitor C70 and the other end of the resistor R60 are connected to the 4th pin of the operational amplifier U5A and the 6th pin of the operational amplifier U5B, the 7th pin of the operational amplifier U5B is connected to one end of the resistor R59 and one end of the resistor R68, the other end of the resistor R68 is connected to the VREF_1V25 power supply, the other end of the resistor R59 is connected to the 8th pin of the operational amplifier U5B and the 6th pin of the chip U34, and the models of the operational amplifier U5A and the operational amplifier U5B are LMC6482AIM;

[0027] Pin 3 of the chip U36 is connected to pins 43 and 19 of the chip U43, pin 4 of the chip U36 is connected to one end of a resistor R61 and pins 40 and 22 of the chip U43, the other end of the resistor R61 is connected to a +3V3 power supply, pin 5 of the chip U36 is connected to one end of a capacitor C69 and to a +3V3 power supply, the other end of the capacitor C69 is grounded, pin 1 of the chip U36 is connected to pin 1 of an op amp U7A and one end of a photocell D13, pin 2 of the op amp U7A is connected to a capacitor C71, and pin 3 of the chip U36 is connected to a +3V3 power supply. The end, one end of the resistor R65 and the other end of the photocell D13, the other end of the capacitor C71 and the other end of the resistor R65 are connected to the 4th pin of the operational amplifier U7A and the 6th pin of the operational amplifier U7B, the 7th pin of the operational amplifier U7B is connected to one end of the resistor R64 and one end of the resistor R67, the other end of the resistor R67 is connected to the VREF_1V25 power supply, the other end of the resistor R64 is connected to the 8th pin of the operational amplifier U7B and the 6th pin of the chip U36, and the models of the operational amplifier U7A and the operational amplifier U7B are LMC6482AIM;

[0028] Pin 3 of the chip U33, pin 3 of the chip U34 and pin 3 of the chip U36 are connected to one end of capacitor C72, one end of capacitor C73, one end of capacitor C74, one end of resistor R66 and one end of transistor Q14, the other end of capacitor C72, the other end of capacitor C73, the other end of capacitor C74 and the other end of transistor Q14 are grounded, and the other end of resistor R66 is connected to the +3V3 power supply.

[0029] Further, the excitation light source driving unit includes MOS tubes Q11, MOS tubes Q12 and MOS tubes Q13, and the models of MOS tubes Q11, MOS tubes Q12 and MOS tubes Q13 are NDC7002N. Pin 1 of MOS tube Q11, pin 1 of MOS tube Q12 and pin 1 of MOS tube Q13 are connected to one end of resistor R83, the other end of resistor R83 is connected to one end of capacitor C84 and pin 4 of operational amplifier U41, pin 1 of operational amplifier U41 is connected to one end of capacitor 63 and one end of resistor R82, the other end of capacitor C63 is grounded, the other end of resistor R82 is connected to one end of resistor R82, the other end of resistor R82 is connected to one end of capacitor C62, one end of resistor R84 and one end of resistor R81, the other end of capacitor C62 and the other end of resistor R84 are grounded, the other end of resistor R81 is connected to pin 15 of chip U43, the other end of capacitor C84 is connected to one end of resistor R86, The other end of the resistor R86 is connected to one end of the resistor R87 and pin 2 of the operational amplifier U41, pin 2 of the MOS tube Q11, pin 2 of the MOS tube Q12 and pin 2 of the MOS tube Q13 are connected to one end of the resistor R90 and the other end of the resistor R87, the other end of the resistor R90 is grounded, pin 3 of the MOS tube Q11 is connected to one end of the resistor R85 and pin 16 of the chip U43, pin 3 of the MOS tube Q12 is connected to one end of the resistor R88 and pin 17 of the chip U43, pin 3 of the MOS tube Q13 is connected to one end of the resistor R89 ​​and pin 18 of the chip U43, the other end of the resistor R85, the other end of the resistor R88 and the other end of the resistor R89 ​​are grounded, pin 6 of the MOS tube Q11 is connected to pin 4 of the light source LED1, pin 6 of the MOS tube Q12 is connected to pin 3 of the light source LED1, pin 6 of the MOS tube Q13 is connected to pin 2 of the light source LED1, and pin 1 of the light source LED1 is connected to the +5V0 power supply.

[0030] Furthermore, the stepper motor drive unit includes a chip U39, the model of the chip U39 is TMC2209, the pin 1 of the chip U39 is connected to the pin 1 of the interface P22, the pin 2 of the interface P22 is connected to the pin 26 of the chip U39, the pin 3 of the interface P22 is connected to the pin 24 of the chip U39, the pin 4 of the interface P22 is connected to the pin 21 of the chip U39, the interface P22 is used for an external motor, the capacitor C80 is connected between the pins 4 and 5 of the chip U39, the pin 6 of the chip U39 is connected to one end of the capacitor C81, and the other end of the capacitor C81 is connected to one end of the capacitor C82 and one end of the capacitor C83 , and connected to the VCC_MO power supply, the other end of capacitor C82 and the other end of capacitor C83 are grounded, pin 8 of chip U39 is connected to one end of resistor R79 and one end of capacitor C79, and the other end of capacitor C79 is grounded, the other end of resistor R79 is connected to one end of resistor R78 and pin 17 of chip U39, pins 7, 9 and 10 of chip U39 are connected to pin 15 of chip U39 and connected to +3V3 power supply, pin 23 of chip U39 is connected to one end of resistor R71, and the other end of resistor R71 is grounded, pin 27 of chip U39 is connected to one end of resistor R72, and the other end of resistor R72 is grounded.

[0031] Pin 16 of the chip U39 is connected to pin 10 of interface P35, pin 19 of the chip U39 is connected to pin 9 of interface P35, pin 2 of the chip U39 is connected to pin 11 of interface P35, pin 14 of the chip U39 is connected to pin 8 of interface P35, pin 8 of interface P35 is connected to one end of resistor R09 and pin 46 of chip U43, the other end of resistor R09 is connected to pin 45 of chip U43, pin 9 of interface P35 is connected to pin 44 of chip U43, pin 10 of interface P35 is connected to pin 41 of chip U43, and pin 11 of interface P35 is connected to pin 38 of chip U43.

[0032] The present invention adopts the above technical solution, and has the following technical effects compared with the prior art:

[0033] 1. One of the biggest functions of PCR detection instruments is to perform thermal cycling on the test samples. The faster the heating and cooling rate during the thermal cycle, the shorter the detection time, and the more accurate the temperature control, the higher the detection accuracy. The thermal cycle process requires heating and cooling, so Peltier is used as the heat pump element of the thermal cycle, which can quickly heat and cool the thermal cycle metal block;

[0034] 2. Different from the existing PWM control method of Peltier, the present invention adopts DC voltage regulation to control Peltier, which can greatly reduce the current ripple of Peltier. Peltier is a device that uses electric current to transfer heat. Stable current will effectively improve the temperature control accuracy. Using DC voltage regulation for temperature control can reduce temperature control fluctuations under the same thermal cycle mechanical structure, improve temperature control accuracy and thus improve detection accuracy.

[0035] 3. The present invention adds an auxiliary heating function. Due to the structural limitations of the thermal circulation tank, a certain temperature difference will be caused. The smaller the temperature difference between different holes, the higher the consistency of the test results. The auxiliary heating function can compensate for the temperature, reduce the temperature difference of the thermal circulation tank, and improve the consistency of the test results.

[0036] 4. The fan control of the present invention uses a brushless fan commonly used in industrial computers. This type of fan has the characteristics of long life and maintenance-free. The fan itself has a PWM speed regulation interface; it has two independent radiators. The fan will only start when the temperature exceeds 35 degrees. When the radiator is at a low temperature, the fan will not run at high speed, reducing equipment noise.

[0037] 5. The excitation light source driving circuit has three excitation light sources with different wavelengths. Only one of the three light sources is started at the same time, so the light source part shares the constant current control circuit, and the three light sources are controlled in time through three composite MOS tubes; the light source LED1 adopts a customized composite tube core, which integrates three LEDs of different bands in one package, and the light intensity of the light source can be infinitely adjusted. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0039] Figure 1 The circuit diagram of the processor unit #1 in the present invention;

[0040] Figure 2 This is a circuit diagram of the 1# auxiliary heating compensation unit in the present invention;

[0041] Figure 3 This is a circuit diagram of the 2# auxiliary heating compensation unit in the present invention;

[0042] Figure 4 The circuit diagram of the semiconductor refrigeration unit No. 1 in the present invention;

[0043] Figure 5 The circuit diagram of the 2# semiconductor refrigeration unit in the present invention;

[0044] Figure 6 is a circuit diagram of a cooling fan control unit in the present invention;

[0045] Figure 7 The circuit diagram of the 2# processor unit in the present invention;

[0046] Figure 8 is a circuit diagram of the fluorescence detection unit in the present invention;

[0047] Fig. 9 is a circuit diagram of the excitation light source driving unit in the present invention;

[0048] Fig.10 and Fig.11 It is a circuit diagram of the stepping motor driving unit in the present invention. DETAILED DESCRIPTION

[0049] Example 1, a control system for PCR detection, including a temperature control module and a fluorescence detection probe control module, the temperature control module is used for temperature control of PCR thermal cycle and signal processing of fluorescence detection, and the fluorescence detection probe control module is used to realize position control of the detection probe and fluorescence signal detection.

[0050] The temperature control module includes a 1# processor unit, a 1# auxiliary heating compensation unit, a 2# auxiliary heating compensation unit, a 1# semiconductor refrigeration unit, a 2# semiconductor refrigeration unit, a power supply and a cooling fan control unit. The processor unit is connected to the 1# auxiliary heating compensation unit, the 2# auxiliary heating compensation unit, the 1# semiconductor refrigeration unit, the 2# semiconductor refrigeration unit, the 1# power supply and the cooling fan control unit. The 1# processor unit is also connected to an Android display and a thermal printer through an interface.

[0051] The fluorescence detection probe control module includes a 2# processor unit, a fluorescence detection unit, an excitation light source driving unit and a stepper motor driving unit, and the 2# processor unit is connected to the fluorescence detection unit, the excitation light source driving unit and the stepper motor driving unit.

[0052] like Figure 1 As shown, the processor unit #1 includes a chip U29, and the model of the chip U29 is HC32F072PATA.

[0053] like Figure 2As shown, the 1# auxiliary heating compensation unit includes a chip U1, the model of the chip U1 is TM7707, the 1st pin of the chip U1 is connected to the 53rd pin of the chip U29, the 2nd pin of the chip U1 is connected to the 52nd pin of the chip U29, the 4th pin of the chip U1 is connected to the 58th pin of the chip U29, the 5th pin of the chip U1 is connected to the 51st pin of the chip U29, the 12th pin of the chip U1 is connected to the 59th pin of the chip U29, the 13th pin of the chip U1 is connected to the 60th pin of the chip U29, the 14th pin of the chip U1 is connected to the 57th pin of the chip U29, and the 6th pin of the chip U1 is connected to the interface P2 Pin 2 of chip U1 and one end of resistor RJ1, the other end of resistor RJ1 is grounded, pin 7 of chip U1 is connected to one end of resistor R1 and pin 1 of interface P2, interface P2 is used for an external temperature sensor, pin 9 of chip U1 is connected to the VOUT pin of chip U2, one end of capacitor C3 and the other end of resistor R1, the other end of capacitor C3 is grounded, the VIN pin and EN pin of chip U2 are connected to one end of capacitor C2, one end of capacitor C1 and pin 15 of chip U1, the other end of capacitor C2 and the other end of capacitor C1 are grounded and connected to +3V3 power supply. The model of chip U2 is XC6209F122MR.

[0054] The 1# auxiliary heating compensation unit also includes a MOS tube Q9, a G pole of the MOS tube Q9 is connected to one end of a resistor R62, the other end of the resistor R62 is grounded, a D pole of the MOS tube Q9 is connected to pin 2 of the interface P15, a pin 1 of the interface P15 is connected to one end of a capacitor C80 and connected to a VIN power supply, and the other end of the capacitor C80 and the S pole of the MOS tube Q9 are grounded.

[0055] like Figure 3 As shown, the 2# auxiliary heating compensation unit includes a chip U3, the model of the chip U3 is TM7707, the 1st pin of the chip U3 is connected to the 53rd pin of the chip U29, the 2nd pin of the chip U3 is connected to the 52nd pin of the chip U29, the 4th pin of the chip U3 is connected to the 58th pin of the chip U29, the 5th pin of the chip U3 is connected to the 51st pin of the chip U29, the 12th pin of the chip U3 is connected to the 62nd pin of the chip U29, the 13th pin of the chip U3 is connected to the 63rd pin of the chip U29, the 14th pin of the chip U3 is connected to the 57th pin of the chip U29, and the 6th pin of the chip U3 is connected to the interface P3 Pin 2 of chip U3 is connected to one end of resistor RJ2, and the other end of resistor RJ2 is grounded. Pin 7 of chip U3 is connected to one end of resistor R2 and pin 1 of interface P3. Interface P3 is used for an external temperature sensor. Pin 9 of chip U3 is connected to the VOUT pin of chip U4, one end of capacitor C6 and the other end of resistor R2, and the other end of capacitor C6 is grounded. The VIN pin and EN pin of chip U4 are connected to one end of capacitor C5, one end of capacitor C4 and pin 15 of chip U3, and are connected to a +3V3 power supply. The other end of capacitor C5 and the other end of capacitor C4 are grounded. The model of chip U4 is XC6209F122MR.

[0056] The 2# auxiliary heating compensation unit also includes a MOS tube Q10, a G pole of the MOS tube Q10 is connected to one end of a resistor R63, the other end of the resistor R63 is grounded, a D pole of the MOS tube Q10 is connected to pin 2 of the interface P16, pin 1 of the interface P16 is connected to one end of a capacitor C81 and connected to a VIN power supply, and the other end of the capacitor C81 and the S pole of the MOS tube Q10 are grounded.

[0057] The TM7707 chip is used for the 24-bit ADC for low-frequency DC signal measurement. The ADC part is used for PT1000 temperature sensor measurement. The circuit uses the proportional method for measurement. The benchmark is the proportional resistor R1. Here, the resistor R1 uses a 0.01% precision metal film resistor with a temperature drift of 10ppm / ℃. The resistance value of the PT1000 to be measured can be calculated by the proportional method: RESPT1000 = (R1 * ADC) / (ADCfull - ADC); where ADC is the TM7707 measurement value, ADCfull is the full-scale ADC value, which is a fixed value of 16777216. After calculating the resistance value of PT1000, the temperature resolution of 0.01℃ can be obtained by table lookup and piecewise linear calculation. The circuit requires temperature accuracy better than ±0.1℃. PT1000 uses 1 / 3DIN accuracy level, and the circuit can be calibration-free. The auxiliary heating heating element is a PI heating film, which is a resistance element. PWM control MOS tube can be used for temperature control.

[0058] like Figure 4 As shown, the 1# semiconductor refrigeration unit includes a chip U6, the model of the chip U6 is MP2905, the 1st pin of the chip U6 is connected to one end of the resistor R5 and one end of the diode D2, the other end of the resistor R5 is connected to the 1st and 4th pins of the operational amplifier U5, the 3rd pin of the operational amplifier U5 is connected to one end of the capacitor C9 and one end of the resistor R4, the other end of the capacitor C9 is grounded, the other end of the resistor R4 is connected to one end of the resistor R3 and one end of the capacitor C8, the other end of the capacitor C8 is grounded, the other end of the resistor R3 is connected to the 44th pin of the chip U29, the other end of the diode D2 is connected to the operational amplifier U Pin 1 of op amp U7 and one end of capacitor C25, pin 3 of op amp U7 is connected to one end of capacitor C22, one end of resistor R9 and one end of resistor R8, pin 2 of op amp U7 and the other end of resistor R9 are connected to +3V3 power supply, the other end of resistor R8 and the other end of capacitor C22 are grounded, pin 4 of op amp U7 is connected to one end of resistor R14 and one end of resistor R15, the other end of resistor R14 is connected to pin 1 of chip U6, the other end of resistor R15 is connected to the other end of capacitor C25, pin 3 of chip U6 is connected to one end of capacitor C20 and one end of diode D3, and the other end of capacitor C20 is grounded.

[0059] Pin 7 of the chip U6 is connected to one end of capacitor C15, the D pole of MOS tube Q2, the S pole of MOS tube Q1, one end of capacitor C18, one end of capacitor C19 and one end of inductor L1. The other end of capacitor C15 is connected to pin 6 of chip U6 and the other end of diode D3. The G pole of MOS tube Q2 is connected to pin 5 of chip U6. The S pole of MOS tube Q2 is grounded. The other end of capacitor C18 is connected to one end of resistor R10. The other end of resistor R10 is grounded. 9 The other end is connected to one end of resistor R12, the other end of resistor R12 is connected to one end of resistor R13 and pin 1 of chip U6, the other end of resistor R13 is grounded, the other end of inductor L1 is connected to one end of resistor R11, one end of capacitor C23 and one end of capacitor C21, and connected to PW_TEC1 power supply, the other end of capacitor C23 and the other end of capacitor C21 are grounded, the other end of resistor R11 is connected to one end of capacitor C24 and pin 1 of chip U6, and the other end of capacitor C24 is grounded.

[0060] Pin 8 of the chip U6 is connected to one end of a resistor R7 and one end of a diode D1, the other end of the resistor R7 and the other end of the diode D1 are connected to the G pole of the MOS tube Q1, pin 9 of the chip U6 is connected to one end of a capacitor C16 and connected to the VIN power supply, the other end of the capacitor C16 is grounded, pin 10 of the chip U6 is connected to one end of a capacitor C13 and one end of a resistor R6, the other end of the capacitor C13 and the other end of the resistor R6 are connected to the D pole of the MOS tube Q1, one end of a capacitor C10, one end of a capacitor C12 and one end of a capacitor C11, and connected to the VIN power supply, the other end of the capacitor C10, the other end of the capacitor C12 and the other end of the capacitor C11 are grounded.

[0061] The 1# semiconductor refrigeration unit also includes a chip U8 and a chip U10. The models of the chip U8 and the chip U10 are both WSP4620. The 2nd foot of the chip U8 is connected to one end of the resistor R19 and the 3rd foot of the optocoupler U9. The other end of the resistor R19 is grounded. The 2nd foot of the optocoupler U9 is connected to one end of the resistor R16. The other end of the resistor R16 is grounded. The 4th foot of the optocoupler U9 is connected to the +5V0 power supply. The 1st foot of the optocoupler U9 is connected to the 45th foot of the chip U29. Pin 4 of chip U8 is connected to pin 4 of optocoupler U12 and one end of resistor R21, the other end of resistor R21 is connected to PW_TEC1 power supply, pin 2 of optocoupler U12 is connected to one end of resistor R22, the other end of resistor R22 is grounded, pin 1 of optocoupler U12 is connected to pin 46 of chip U29, pin 3 of optocoupler U12 is connected to pin 3 of chip U15, pin 2 of chip U10 is connected to one end of resistor R18 and pin 3 of optocoupler U11, resistor R18 is grounded, and the other end of resistor R22 is grounded. The other end is grounded, pin 2 of the optocoupler U11 is connected to one end of the resistor R17, the other end of the resistor R17 is grounded, pin 4 of the optocoupler U11 is connected to the +5V0 power supply, pin 1 of the optocoupler U11 is connected to pin 47 of the chip U29, pin 4 of the chip U10 is connected to pin 4 of the optocoupler U14 and one end of the resistor R20, the other end of the resistor R20 is connected to the PW_TEC1 power supply, pin 2 of the optocoupler U14 is connected to one end of the resistor R23, the other end of the resistor R23 is grounded, Pin 1 of the optocoupler U14 is connected to pin 49 of the chip U29, pin 3 of the optocoupler U14 is connected to pin 3 of the chip U15, the model of the chip U15 is B0509S-1W, pin 2 of the chip U15 is connected to one end of the capacitor C27 and to the +5V0 power supply, the other end of the capacitor C27 is grounded, pin 4 of the chip U15 is connected to one end of the capacitor C28 and to the PW_TEC1 power supply, the other end of the capacitor C28 is connected to pin 3 of the chip U15.

[0062] The 1# semiconductor refrigeration unit also includes a chip U13, the model of which is TM7707, the 1st pin of the chip U13 is connected to the 53rd pin of the chip U29, the 2nd pin of the chip U13 is connected to the 52nd pin of the chip U29, the 4th pin of the chip U13 is connected to the 54th pin of the chip U29, the 5th pin of the chip U13 is connected to the 51st pin of the chip U29, the 12th pin of the chip U13 is connected to the 55th pin of the chip U29, the 13th pin of the chip U13 is connected to the 56th pin of the chip U29, the 14th pin of the chip U13 is connected to the 57th pin of the chip U29, the 6th pin of the chip U13 is connected to the 2nd pin of the interface P5 and One end of resistor RJ3 and the other end of resistor RJ3 are grounded, pin 7 of chip U13 is connected to one end of resistor R24 ​​and pin 1 of interface P5, interface P5 is used for an external temperature sensor, pin 9 of chip U13 is connected to the VOUT pin of chip U16, one end of capacitor C31 and the other end of resistor R24, the other end of capacitor C31 is grounded, the VIN pin and EN pin of chip U16 are connected to one end of capacitor C30, one end of capacitor C29 and pin 15 of chip U13, and connected to +3V3 power supply, the other end of capacitor C29 and the other end of capacitor C30 are grounded, and the model of chip U16 is XC6209F122MR.

[0063] like Figure 5 As shown, the 2# semiconductor refrigeration unit includes a chip U18, the model of the chip U18 is MP2905, the 1st pin of the chip U18 is connected to one end of the resistor R27 and one end of the diode D5, the other end of the resistor R27 is connected to the 1st and 4th pins of the operational amplifier U17, the 3rd pin of the operational amplifier U17 is connected to one end of the capacitor C34 and one end of the resistor R26, the other end of the capacitor C34 is grounded, the other end of the resistor R26 is connected to one end of the resistor R25 and one end of the capacitor C33, the other end of the capacitor C33 is grounded, the other end of the resistor R25 is connected to the 77th pin of the chip U29, the other end of the diode D5 is connected to Pin 1 of op amp U19 is connected to one end of capacitor C50, pin 3 of op amp U19 is connected to one end of capacitor C47, one end of resistor R30 and one end of resistor R31, pin 2 of op amp U19 and the other end of resistor R31 are connected to +3V3 power supply, the other end of resistor R30 and the other end of capacitor C47 are grounded, pin 4 of op amp U19 is connected to one end of resistor R36 and one end of resistor R37, the other end of resistor R36 is connected to pin 1 of chip U18, the other end of resistor R37 is connected to the other end of capacitor C50, pin 3 of chip U18 is connected to one end of capacitor C45 and one end of diode D6, and the other end of capacitor C45 is grounded.

[0064] Pin 7 of the chip U18 is connected to one end of capacitor C40, the D pole of MOS tube Q4, the S pole of MOS tube Q3, one end of capacitor C43, one end of capacitor C44 and one end of inductor L2. The other end of capacitor C40 is connected to pin 6 of chip U18 and the other end of diode D6. The G pole of MOS tube Q4 is connected to pin 5 of chip U18. The S pole of MOS tube Q4 is grounded. The other end of capacitor C43 is connected to one end of resistor R32. The other end of resistor R32 is grounded. The other end of 44 is connected to one end of resistor R34, the other end of resistor R34 is connected to one end of resistor R35 and pin 1 of chip U18, the other end of resistor R35 is grounded, the other end of inductor L2 is connected to one end of resistor R33, one end of capacitor C48 and one end of capacitor C46, ​​and connected to PW_TEC2 power supply, the other end of capacitor C48 and the other end of capacitor C46 are grounded, the other end of resistor R33 is connected to one end of capacitor C49 and pin 1 of chip U18, the other end of capacitor C49 is grounded.

[0065] Pin 8 of the chip U18 is connected to one end of a resistor R29 and one end of a diode D4, the other end of the resistor R29 and the other end of the diode D4 are connected to the G pole of the MOS tube Q3, pin 9 of the chip U18 is connected to one end of a capacitor C41 and connected to the VIN power supply, the other end of the capacitor C41 is grounded, pin 10 of the chip U18 is connected to one end of a capacitor C38 and one end of a resistor R28, the other end of the capacitor C38 and the other end of the resistor R28 are connected to the D pole of the MOS tube Q3, one end of a capacitor C35, one end of a capacitor C37 and one end of a capacitor C36, and connected to the VIN power supply, the other end of the capacitor C35, the other end of the capacitor C37 and the other end of the capacitor C36 are grounded.

[0066] The 2# semiconductor refrigeration unit also includes a chip U20 and a chip U22. The models of the chips U20 and U22 are both WSP4620. The 2nd pin of the chip U20 is connected to one end of the resistor R41 and the 3rd pin of the optocoupler U21. The other end of the resistor R41 is grounded. The 2nd pin of the optocoupler U21 is connected to one end of the resistor R38. The other end of the resistor R38 is grounded. The 4th pin of the optocoupler U21 is connected to the +5V0 power supply. The 1st pin of the optocoupler U21 is connected to the chip U29. Pin 78 of chip U20, pin 4 of chip U20 is connected to pin 4 of optocoupler U24 and one end of resistor R43, the other end of resistor R43 is connected to PW_TEC2 power supply, pin 2 of optocoupler U23 is connected to one end of resistor R44, the other end of resistor R44 is grounded, pin 1 of optocoupler U23 is connected to pin 79 of chip U29, pin 3 of optocoupler U23 is connected to pin 3 of chip U27, pin 2 of chip U22 is connected to one end of resistor R40 and pin 3 of optocoupler U23, The other end of resistor R40 is grounded, pin 2 of optocoupler U23 is connected to one end of resistor R39, the other end of resistor R39 is grounded, pin 4 of optocoupler U23 is connected to +5V0 power supply, pin 1 of optocoupler U23 is connected to pin 80 of chip U29, pin 4 of chip U22 is connected to pin 4 of optocoupler U26 and one end of resistor R42, the other end of resistor R42 is connected to PW_TEC2 power supply, pin 2 of optocoupler U26 is connected to one end of resistor R45, the other end of resistor R45 is connected Ground, pin 1 of optocoupler U26 is connected to pin 81 of chip U29, pin 3 of optocoupler U26 is connected to pin 3 of chip U27, the model of chip U27 is B0509S-1W, pin 2 of chip U27 is connected to one end of capacitor C52 and to +5V0 power supply, the other end of capacitor C52 is grounded, pin 4 of chip U27 is connected to one end of capacitor C53 and to PW_TEC2 power supply, the other end of capacitor C53 is connected to pin 3 of chip U27.

[0067] The 2# semiconductor refrigeration unit also includes a chip U25, the model of which is TM7707, pin 1 of the chip U25 is connected to pin 53 of the chip U29, pin 2 of the chip U25 is connected to pin 52 of the chip U29, pin 4 of the chip U25 is connected to pin 64 of the chip U29, pin 5 of the chip U25 is connected to pin 51 of the chip U29, pin 12 of the chip U25 is connected to pin 65 of the chip U29, pin 13 of the chip U25 is connected to pin 66 of the chip U29, pin 14 of the chip U25 is connected to pin 57 of the chip U29, and pin 6 of the chip U25 is connected to pin 2 of the interface P7 and One end of resistor RJ4 and the other end of resistor RJ4 are grounded, pin 7 of chip U25 is connected to one end of resistor R46 and pin 1 of interface P7, interface P7 is used for an external temperature sensor, pin 9 of chip U25 is connected to the VOUT pin of chip U28, one end of capacitor C56 and the other end of resistor R46, the other end of capacitor C56 is grounded, the VIN pin and EN pin of chip U28 are connected to one end of capacitor C55, one end of capacitor C54 and pin 15 of chip U25, and connected to a +3V3 power supply, the other end of capacitor C55 and the other end of capacitor C54 are grounded, and the model of chip U28 is XC6209F122MR.

[0068] The temperature control of the PCR thermal cycle uses two 80-watt Peltiers as the core temperature control elements. When controlling cooling, the maximum total cooling capacity is 80 watts, and when controlling heating, the maximum total heating capacity is 240W, which can quickly raise and lower the temperature of the thermal cycle metal block.

[0069] Peltier is a structure of different metals pressed together. Internal stress needs to be considered during control to prevent damage caused by desoldering of the metal layer. Usually, fast intermittent current (i.e. PWM method) cannot be used to control temperature, and low-speed (cycle greater than 2 seconds) PWM control method is completely unsuitable for applications with rapid temperature rise and fall. Therefore, Peltier control mostly uses DC method to achieve different thermal power output by controlling the voltage across the Peltier.

[0070] The synchronous rectifier chip MP2905 is controlled by TEC1_DAC and TEC2_DAC to generate an adjustable voltage PW_TEC1 of 0-13V. This voltage is connected to the Peltier through the H-bridge to achieve the control of different output powers of the Peltier. The power adjustment adopts continuous DC control. The Peltier will not vibrate due to the discontinuous current, which can extend the service life of the device. The effective accuracy of DAC can reach 12 bits, and the adjustment accuracy of PW_TEC1 and PW_TEC2 can reach 3.18mV, which greatly improves the temperature control accuracy. The temperature acquisition still uses TM7707 and is calculated by the proportional method. The principle is the same as that in the auxiliary heating compensation unit.

[0071] like Figure 6As shown, the cooling fan control unit includes a chip U30 and a chip U32. The models of the chip U30 and the chip U32 are TC1047AVNBTR. Pin 1 of the chip U30 is connected to one end of a capacitor C58 and to a +3V3 power supply. The other end of the capacitor C58 is grounded. Pin 3 of the chip U30 is connected to pin 35 of the chip U29. Pin 1 of the chip U32 is connected to one end of a capacitor C62 and to a +3V3 power supply. The other end of the capacitor C62 is grounded. Pin 3 of the chip U32 is connected to pin 37 of the chip U29.

[0072] The cooling fan control unit also includes a MOS tube Q5 and a MOS tube Q6, wherein the G pole of the MOS tube Q5 is connected to one end of a resistor R49 and pin 29 of a chip U29, the S pole of the MOS tube Q5 and the other end of the resistor R49 are grounded, the D pole of the MOS tube Q5 is connected to pin 3 of an interface P10, pin 2 of the interface P10 is connected to a VIN power supply, and pin 4 of the interface P10 is connected to pin 29 of the chip U29; the G pole of the MOS tube Q6 is connected to one end of a resistor R50 and pin 91 of the chip U29, the S pole of the MOS tube Q6 and the other end of the resistor R50 are grounded, the D pole of the MOS tube Q6 is connected to pin 3 of an interface P11, pin 2 of the interface P11 is connected to a VIN power supply, and pin 4 of the interface P11 is connected to pin 91 of the chip U29.

[0073] The control system described in the present invention uses a brushless fan commonly used in industrial computers. This type of fan has the characteristics of long life and maintenance-free. The fan itself has a PWM speed regulation interface. By connecting the PWM signal through the fourth pin of the P10 terminal, the fan speed can be controlled.

[0074] In order to reduce the noise of the device, the fan will not run at high speed when the radiator is at a low temperature. The device has two independent radiators. There are two TC1047 temperature sensors on the back of the main control board to detect the radiator temperature respectively. When the temperature exceeds 35 degrees, the fan will start and increase the speed according to the temperature value.

[0075] TC1047 is an analog output temperature sensor with a range of -40 to 125°C. The maximum temperature error within the full range is 2°C. For the temperature control of the radiator, this accuracy does not require temperature calibration.

[0076] like Figure 7 As shown, the 2# processor unit includes a chip U43, and the model of the chip U43 is HC32F030J8TA.

[0077] like Figure 8As shown, the fluorescence detection unit includes a chip U33, a chip U34 and a chip U36. The models of the chips U33, U34 and U36 are all MCP3421. The 3rd pin of the chip U33 is connected to one end of a resistor R51 and the 43rd and 19th pins of the chip U43. The other end of the resistor R51 is connected to a +3V3 power supply. The 4th pin of the chip U33 is connected to one end of a resistor R52 and the 42nd and 20th pins of the chip U43. The other end of the resistor R52 is connected to a +3V3 power supply. The 5th pin of the chip U33 is connected to one end of a capacitor C66 and connected to a +3V3 power supply. The other end of the capacitor C66 is grounded. Pin 1 of 3 is connected to pin 1 of op amp U2A and one end of photocell D11, pin 2 of op amp U2A is connected to one end of capacitor C67, one end of resistor R55 and the other end of photocell D11, the other end of capacitor C67 and the other end of resistor R55 are connected to pin 4 of op amp U2A and pin 6 of op amp U2B, pin 7 of op amp U2B is connected to one end of resistor R54 and one end of resistor R56, the other end of resistor R56 is connected to VREF_1V25 power supply, the other end of resistor R54 is connected to pin 8 of op amp U2B and pin 6 of chip U33, and the models of op amp U2A and op amp U2B are LMC6482AIM.

[0078] The 3rd pin of the chip U34 is connected to the 43rd pin and the 19th pin of the chip U43, the 4th pin of the chip U34 is connected to one end of the resistor R57 and the 41st pin and the 21st pin of the chip U43, the other end of the resistor R57 is connected to the +3V3 power supply, the 5th pin of the chip U34 is connected to one end of the capacitor C68 and the +3V3 power supply, the other end of the capacitor C68 is grounded, the 1st pin of the chip U34 is connected to the 1st pin of the operational amplifier U5A and one end of the photocell D12, the 2nd pin of the operational amplifier U5A is connected to the capacitor C70, The pin 7 of the operational amplifier U5B is connected to one end of the resistor R59 and one end of the resistor R68. The other end of the resistor R68 is connected to the VREF_1V25 power supply. The other end of the resistor R59 is connected to the pin 8 of the operational amplifier U5B and the pin 6 of the chip U34. The models of the operational amplifier U5A and the operational amplifier U5B are LMC6482AIM.

[0079] Pin 3 of the chip U36 is connected to pins 43 and 19 of the chip U43, pin 4 of the chip U36 is connected to one end of a resistor R61 and pins 40 and 22 of the chip U43, the other end of the resistor R61 is connected to a +3V3 power supply, pin 5 of the chip U36 is connected to one end of a capacitor C69 and to a +3V3 power supply, the other end of the capacitor C69 is grounded, pin 1 of the chip U36 is connected to pin 1 of an op amp U7A and one end of a photocell D13, pin 2 of the op amp U7A is connected to a capacitor C71, and pin 3 of the chip U36 is connected to a +3V3 power supply. The first end, one end of the resistor R65 and the other end of the photocell D13, the other end of the capacitor C71 and the other end of the resistor R65 are connected to the 4-pin of the op amp U7A and the 6-pin of the op amp U7B, the 7-pin of the op amp U7B is connected to one end of the resistor R64 and one end of the resistor R67, the other end of the resistor R67 is connected to the VREF_1V25 power supply, the other end of the resistor R64 is connected to the 8-pin of the op amp U7B and the 6-pin of the chip U36, and the models of the op amp U7A and the op amp U7B are LMC6482AIM.

[0080] Pin 3 of the chip U33, pin 3 of the chip U34 and pin 3 of the chip U36 are connected to one end of capacitor C72, one end of capacitor C73, one end of capacitor C74, one end of resistor R66 and one end of transistor Q14, the other end of capacitor C72, the other end of capacitor C73, the other end of capacitor C74 and the other end of transistor Q14 are grounded, and the other end of resistor R66 is connected to the +3V3 power supply.

[0081] In order to realize fluorescence detection in three different bands, three different narrow-band filters are needed to filter the excitation light source. After passing through the three different narrow-band filters, the fluorescence enters the photocells D11, D12, and D13, and after passing through the transimpedance amplifier composed of resistors R55, R60, R65 and the operational amplifier, a low-impedance DC signal is generated. Then MCP3421 collects the electrical signal as a digital signal to complete the fluorescence signal collection.

[0082] Among them, LMC6482 is an op amp with ultra-low bias current and input current as low as 20fA, which has a very small impact on the measurement of weak photoelectric signals. MCP3421 is a high-precision ADC chip, which can achieve 18-bit data output at a rate of 3.75sps and 16-bit data output at a rate of 15sps, which is very suitable for small signal measurement.

[0083] like Fig. 9As shown, the excitation light source driving unit includes MOS tubes Q11, MOS tubes Q12 and MOS tubes Q13, and the models of MOS tubes Q11, MOS tubes Q12 and MOS tubes Q13 are NDC7002N. Pin 1 of MOS tube Q11, pin 1 of MOS tube Q12 and pin 1 of MOS tube Q13 are connected to one end of resistor R83, and the other end of resistor R83 is connected to one end of capacitor C84 and pin 4 of operational amplifier U41. Pin 1 of operational amplifier U41 is connected to one end of capacitor 63 and one end of resistor R82, and the other end of capacitor C63 is grounded. The other end of resistor R82 is connected to one end of resistor R82, and the other end of resistor R82 is connected to one end of capacitor C62, one end of resistor R84 and one end of resistor R81. The other end of capacitor C62 and the other end of resistor R84 are grounded. The other end of resistor R81 is connected to pin 15 of chip U43, and the other end of capacitor C84 is connected to one end of resistor R86. The other end of resistor R86 is connected to one end of resistor R87 and pin 2 of operational amplifier U41, pin 2 of MOS tube Q11, pin 2 of MOS tube Q12 and pin 2 of MOS tube Q13 are connected to one end of resistor R90 and the other end of resistor R87, the other end of resistor R90 is grounded, pin 3 of MOS tube Q11 is connected to one end of resistor R85 and pin 16 of chip U43, pin 3 of MOS tube Q12 is connected to one end of resistor R88 and pin 17 of chip U43, pin 3 of MOS tube Q13 is connected to one end of resistor R89 ​​and pin 18 of chip U43, the other end of resistor R85, the other end of resistor R88 and the other end of resistor R89 ​​are grounded, pin 6 of MOS tube Q11 is connected to pin 4 of light source LED1, pin 6 of MOS tube Q12 is connected to pin 3 of light source LED1, pin 6 of MOS tube Q13 is connected to pin 2 of light source LED1, and pin 1 of light source LED1 is connected to +5V0 power supply.

[0084] In order to achieve compatibility with a variety of detection reagents, the excitation light source driving unit has three excitation light sources with different wavelengths. Only one of the three light sources is activated at the same time, so the light source part shares the constant current control circuit, and the three light sources are controlled in time through the three composite MOS tubes of MOS tube Q11, MOS tube Q12, and MOS tube Q13. The light source LED1 uses a customized composite tube core to integrate three LEDs of different wavelengths in one package to simplify the lens design. The light intensity of the light source can also be infinitely adjusted by adjusting the LED_PWM signal of the program.

[0085] like Fig.10As shown, the stepper motor drive unit includes a chip U39, the model of the chip U39 is TMC2209, the pin 1 of the chip U39 is connected to the pin 1 of the interface P22, the pin 2 of the interface P22 is connected to the pin 26 of the chip U39, the pin 3 of the interface P22 is connected to the pin 24 of the chip U39, the pin 4 of the interface P22 is connected to the pin 21 of the chip U39, the interface P22 is used for an external motor, the capacitor C80 is connected between the pins 4 and 5 of the chip U39, the pin 6 of the chip U39 is connected to one end of the capacitor C81, and the other end of the capacitor C81 is connected to one end of the capacitor C82 and one end of the capacitor C83. VCC_MO power supply is connected in parallel, the other end of capacitor C82 and the other end of capacitor C83 are grounded, pin 8 of chip U39 is connected to one end of resistor R79 and one end of capacitor C79, the other end of capacitor C79 is grounded, the other end of resistor R79 is connected to one end of resistor R78 and pin 17 of chip U39, pins 7, 9 and 10 of chip U39 are connected to pin 15 of chip U39 and connected to +3V3 power supply, pin 23 of chip U39 is connected to one end of resistor R71, the other end of resistor R71 is grounded, pin 27 of chip U39 is connected to one end of resistor R72, and the other end of resistor R72 is grounded.

[0086] Pin 16 of the chip U39 is connected to pin 10 of interface P35, pin 19 of the chip U39 is connected to pin 9 of interface P35, pin 2 of the chip U39 is connected to pin 11 of interface P35, pin 14 of the chip U39 is connected to pin 8 of interface P35, pin 8 of interface P35 is connected to one end of resistor R09 and pin 46 of chip U43, the other end of resistor R09 is connected to pin 45 of chip U43, pin 9 of interface P35 is connected to pin 44 of chip U43, pin 10 of interface P35 is connected to pin 41 of chip U43, and pin 11 of interface P35 is connected to pin 38 of chip U43.

[0087] like Fig.11As shown, the stepper motor drive unit also includes a chip U48, the model of the chip U48 is TMC2209, the pin 1 of the chip U48 is connected to the pin 1 of the interface P43, the pin 2 of the interface P43 is connected to the pin 26 of the chip U48, the pin 3 of the interface P43 is connected to the pin 24 of the chip U48, the pin 4 of the interface P43 is connected to the pin 21 of the chip U48, the interface P43 is used for an external motor, the capacitor C96 is connected between the pins 4 and 5 of the chip U48, the pin 6 of the chip U48 is connected to one end of the capacitor C97, the other end of the capacitor C97 is connected to one end of the capacitor C98 and one end of the capacitor C99, VCC_MO power supply is connected in parallel, the other end of capacitor C98 and the other end of capacitor C99 are grounded, pin 8 of chip U48 is connected to one end of resistor R91 and one end of capacitor C95, the other end of capacitor C95 is grounded, the other end of resistor R91 is connected to one end of resistor R98 and pin 17 of chip U48, pins 7, 9 and 10 of chip U48 are connected to pin 15 of chip U48 and connected to +3V3 power supply, pin 23 of chip U48 is connected to one end of resistor R99, the other end of resistor R99 is grounded, pin 27 of chip U48 is connected to one end of resistor R90, and the other end of resistor R90 is grounded.

[0088] Pin 16 of the chip U48 is connected to pin 40 of the chip U43, pin 19 of the chip U48 is connected to pin 39 of the chip U43, pin 2 of the chip U48 is connected to pin 38 of the chip U43, pin 14 of the chip U48 is connected to pin 43 of the chip U43 and one end of the resistor R92, and the other end of the resistor R92 is connected to pin 42 of the chip U43.

[0089] The fluorescence detection probe control module is a small module with an area of ​​only 5cm*5cm. At the same time, the stepper motor needs to be fixed on the PCB. The effective area of ​​the PCB is very small. The stepper motor driver chooses the TMC2209 integrated driver chip. The chip can complete the circuit layout in a space of 1cm*1cm.

[0090] The description of the present invention is given for the purpose of illustration and description, and is not intended to be exhaustive or to limit the invention to the disclosed form. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention and to enable those of ordinary skill in the art to understand the present invention and thereby design various embodiments with various modifications suitable for particular uses.

Claims

1. A control system for PCR detection, characterized in that: It includes a temperature control module and a fluorescence detection probe control module. The temperature control module is used for temperature control of PCR thermal cycle and signal processing of fluorescence detection. The fluorescence detection probe control module is used for position control of the detection probe and fluorescence signal detection. The temperature control module includes a 1# processor unit, a 1# auxiliary heating compensation unit, a 2# auxiliary heating compensation unit, a 1# semiconductor refrigeration unit, a 2# semiconductor refrigeration unit, a power supply unit and a cooling fan control unit, the 1# processor unit is connected to the 1# auxiliary heating compensation unit, the 2# auxiliary heating compensation unit, the 1# semiconductor refrigeration unit, the 2# semiconductor refrigeration unit, the 1# power supply and the cooling fan control unit, and the 1# processor unit is also connected to an Android display and a thermal printer through an interface; The fluorescence detection probe control module includes a 2# processor unit, a fluorescence detection unit, an excitation light source driving unit and a stepper motor driving unit, and the 2# processor unit is connected to the fluorescence detection unit, the excitation light source driving unit and the stepper motor driving unit; The 1# semiconductor refrigeration unit includes a chip U6, the model of the chip U6 is MP2905, the 1st pin of the chip U6 is connected to one end of the resistor R5 and one end of the diode D2, the other end of the resistor R5 is connected to the 1st and 4th pins of the operational amplifier U5, the model of the operational amplifier U5 is LMV321IDBVR, the 3rd pin of the operational amplifier U5 is connected to one end of the capacitor C9 and one end of the resistor R4, the other end of the capacitor C9 is grounded, the other end of the resistor R4 is connected to one end of the resistor R3 and one end of the capacitor C8, the other end of the capacitor C8 is grounded, the other end of the resistor R3 is connected to the 44th pin of the chip U29, the other end of the diode D2 is connected to the 1st pin of the operational amplifier U7 and one end of the capacitor C25 The model of chip U29 is HC32F072PATA, pin 3 of op amp U7 is connected to one end of capacitor C22, one end of resistor R9 and one end of resistor R8, pin 2 of op amp U7 and the other end of resistor R9 are connected to +3V3 power supply, the other end of resistor R8 and the other end of capacitor C22 are grounded, the model of op amp U7 is LMV321IDBVR, pin 4 of op amp U7 is connected to one end of resistor R14 and one end of resistor R15, the other end of resistor R14 is connected to pin 1 of chip U6, the other end of resistor R15 is connected to the other end of capacitor C25, pin 3 of chip U6 is connected to one end of capacitor C20 and one end of diode D3, the other end of capacitor C20 is grounded; Pin 7 of the chip U6 is connected to one end of capacitor C15, the D pole of MOS tube Q2, the S pole of MOS tube Q1, one end of capacitor C18, one end of capacitor C19 and one end of inductor L1. The other end of capacitor C15 is connected to pin 6 of chip U6 and the other end of diode D3. The G pole of MOS tube Q2 is connected to pin 5 of chip U6. The S pole of MOS tube Q2 is grounded. The other end of capacitor C18 is connected to one end of resistor R10. The other end of resistor R10 is grounded. 9 The other end is connected to one end of a resistor R12, the other end of the resistor R12 is connected to one end of a resistor R13 and pin 1 of the chip U6, the other end of the resistor R13 is grounded, the other end of the inductor L1 is connected to one end of a resistor R11, one end of a capacitor C23 and one end of a capacitor C21, and connected to the PW_TEC1 power supply, the other end of the capacitor C23 and the other end of the capacitor C21 are grounded, the other end of the resistor R11 is connected to one end of a capacitor C24 and pin 1 of the chip U6, the other end of the capacitor C24 is grounded; The 8th pin of the chip U6 is connected to one end of the resistor R7 and one end of the diode D1, the other end of the resistor R7 and the other end of the diode D1 are connected to the G pole of the MOS tube Q1, the 9th pin of the chip U6 is connected to one end of the capacitor C16, and is connected to the VIN power supply, and the other end of the capacitor C16 is grounded, the 10th pin of the chip U6 is connected to one end of the capacitor C13 and one end of the resistor R6, the other end of the capacitor C13 and the other end of the resistor R6 are connected to the D pole of the MOS tube Q1, one end of the capacitor C10, one end of the capacitor C12 and one end of the capacitor C11, and are connected to the VIN power supply, and the other end of the capacitor C10, the other end of the capacitor C12 and the other end of the capacitor C11 are grounded; The 1# semiconductor refrigeration unit also includes a chip U8 and a chip U10. The models of the chip U8 and the chip U10 are both WSP4620. The 2nd foot of the chip U8 is connected to one end of the resistor R19 and the 3rd foot of the optocoupler U9. The other end of the resistor R19 is grounded. The 2nd foot of the optocoupler U9 is connected to one end of the resistor R16. The other end of the resistor R16 is grounded. The 4th foot of the optocoupler U9 is connected to the +5V0 power supply. The 1st foot of the optocoupler U9 is connected to the 45th foot of the chip U29. Pin 4 of chip U8 is connected to pin 4 of optocoupler U12 and one end of resistor R21, the other end of resistor R21 is connected to PW_TEC1 power supply, pin 2 of optocoupler U12 is connected to one end of resistor R22, the other end of resistor R22 is grounded, pin 1 of optocoupler U12 is connected to pin 46 of chip U29, pin 3 of optocoupler U12 is connected to pin 3 of chip U15, pin 2 of chip U10 is connected to one end of resistor R18 and pin 3 of optocoupler U11, resistor R18 is grounded, and the other end of resistor R22 is grounded. The other end is grounded, pin 2 of the optocoupler U11 is connected to one end of the resistor R17, the other end of the resistor R17 is grounded, pin 4 of the optocoupler U11 is connected to the +5V0 power supply, pin 1 of the optocoupler U11 is connected to pin 47 of the chip U29, pin 4 of the chip U10 is connected to pin 4 of the optocoupler U14 and one end of the resistor R20, the other end of the resistor R20 is connected to the PW_TEC1 power supply, pin 2 of the optocoupler U14 is connected to one end of the resistor R23, the other end of the resistor R23 is grounded, Pin 1 of the optocoupler U14 is connected to pin 49 of the chip U29, pin 3 of the optocoupler U14 is connected to pin 3 of the chip U15, the model of the chip U15 is B0509S-1W, pin 2 of the chip U15 is connected to one end of the capacitor C27 and connected to the +5V0 power supply, the other end of the capacitor C27 is grounded, pin 4 of the chip U15 is connected to one end of the capacitor C28 and connected to the PW_TEC1 power supply, the other end of the capacitor C28 is connected to pin 3 of the chip U15; The 1# semiconductor refrigeration unit also includes a chip U13, the model of which is TM7707, the 1st pin of the chip U13 is connected to the 53rd pin of the chip U29, the 2nd pin of the chip U13 is connected to the 52nd pin of the chip U29, the 4th pin of the chip U13 is connected to the 54th pin of the chip U29, the 5th pin of the chip U13 is connected to the 51st pin of the chip U29, the 12th pin of the chip U13 is connected to the 55th pin of the chip U29, the 13th pin of the chip U13 is connected to the 56th pin of the chip U29, the 14th pin of the chip U13 is connected to the 57th pin of the chip U29, the 6th pin of the chip U13 is connected to the 2nd pin of the interface P5 and One end of the resistor RJ3 and the other end of the resistor RJ3 are grounded, the 7th pin of the chip U13 is connected to one end of the resistor R24 ​​and the 1st pin of the interface P5, the interface P5 is used for an external temperature sensor, the 9th pin of the chip U13 is connected to the VOUT pin of the chip U16, one end of the capacitor C31 and the other end of the resistor R24, the other end of the capacitor C31 is grounded, the VIN pin and the EN pin of the chip U16 are connected to one end of the capacitor C30, one end of the capacitor C29 and the 15th pin of the chip U13, and are connected to the +3V3 power supply, the other end of the capacitor C29 and the other end of the capacitor C30 are grounded, and the model of the chip U16 is XC6209F122MR; The 1# auxiliary heating compensation unit includes a chip U1, the model of the chip U1 is TM7707, the pin 1 of the chip U1 is connected to the pin 53 of the chip U29, the pin 2 of the chip U1 is connected to the pin 52 of the chip U29, the pin 4 of the chip U1 is connected to the pin 58 of the chip U29, the pin 5 of the chip U1 is connected to the pin 51 of the chip U29, the pin 12 of the chip U1 is connected to the pin 59 of the chip U29, the pin 13 of the chip U1 is connected to the pin 60 of the chip U29, the pin 14 of the chip U1 is connected to the pin 57 of the chip U29, and the pin 6 of the chip U1 is connected to the pin 2 of the interface P2. The 7th pin of chip U1 is connected to one end of resistor RJ1 and the 1st pin of interface P2. Interface P2 is used for external temperature sensor. The 9th pin of chip U1 is connected to the VOUT pin of chip U2, one end of capacitor C3 and the other end of resistor R1. The other end of capacitor C3 is grounded. The VIN pin and EN pin of chip U2 are connected to one end of capacitor C2, one end of capacitor C1 and the 15th pin of chip U1 and connected to +3V3 power supply. The other end of capacitor C2 and the other end of capacitor C1 are grounded. The model of chip U2 is XC6209F122MR. The 1# auxiliary heating compensation unit also includes a MOS tube Q9, a G pole of the MOS tube Q9 is connected to one end of a resistor R62, the other end of the resistor R62 is grounded, a D pole of the MOS tube Q9 is connected to pin 2 of the interface P15, a pin 1 of the interface P15 is connected to one end of a capacitor C80 and connected to a VIN power supply, and the other end of the capacitor C80 and the S pole of the MOS tube Q9 are grounded; The fluorescence detection unit includes a chip U33, a chip U34 and a chip U36. The models of the chips U33, U34 and U36 are all MCP3421. The 3rd pin of the chip U33 is connected to one end of a resistor R51 and the 43rd and 19th pins of the chip U43. The other end of the resistor R51 is connected to a +3V3 power supply. The model of the chip U43 is HC32F030J8TA. The 4th pin of the chip U33 is connected to one end of a resistor R52 and the 42nd and 20th pins of the chip U43. The other end of the resistor R52 is connected to a +3V3 power supply. The 5th pin of the chip U33 is connected to one end of a capacitor C66 and connected to a +3V3 power supply. The other end of the capacitor C66 is connected to a +3V3 power supply. One end is grounded, pin 1 of chip U33 is connected to pin 1 of op amp U2A and one end of photocell D11, pin 2 of op amp U2A is connected to one end of capacitor C67, one end of resistor R55 and the other end of photocell D11, the other end of capacitor C67 and the other end of resistor R55 are connected to pin 4 of op amp U2A and pin 6 of op amp U2B, pin 7 of op amp U2B is connected to one end of resistor R54 and one end of resistor R56, the other end of resistor R56 is connected to VREF_1V25 power supply, the other end of resistor R54 is connected to pin 8 of op amp U2B and pin 6 of chip U33, the models of op amp U2A and op amp U2B are LMC6482AIM; The 3rd pin of the chip U34 is connected to the 43rd pin and the 19th pin of the chip U43, the 4th pin of the chip U34 is connected to one end of the resistor R57 and the 41st pin and the 21st pin of the chip U43, the other end of the resistor R57 is connected to the +3V3 power supply, the 5th pin of the chip U34 is connected to one end of the capacitor C68 and the +3V3 power supply, the other end of the capacitor C68 is grounded, the 1st pin of the chip U34 is connected to the 1st pin of the operational amplifier U5A and one end of the photocell D12, the 2nd pin of the operational amplifier U5A is connected to the capacitor C70, The end, one end of the resistor R60 and the other end of the photocell D12, the other end of the capacitor C70 and the other end of the resistor R60 are connected to the 4th pin of the operational amplifier U5A and the 6th pin of the operational amplifier U5B, the 7th pin of the operational amplifier U5B is connected to one end of the resistor R59 and one end of the resistor R68, the other end of the resistor R68 is connected to the VREF_1V25 power supply, the other end of the resistor R59 is connected to the 8th pin of the operational amplifier U5B and the 6th pin of the chip U34, and the models of the operational amplifier U5A and the operational amplifier U5B are LMC6482AIM; Pin 3 of the chip U36 is connected to pins 43 and 19 of the chip U43, pin 4 of the chip U36 is connected to one end of a resistor R61 and pins 40 and 22 of the chip U43, the other end of the resistor R61 is connected to a +3V3 power supply, pin 5 of the chip U36 is connected to one end of a capacitor C69 and to a +3V3 power supply, the other end of the capacitor C69 is grounded, pin 1 of the chip U36 is connected to pin 1 of an op amp U7A and one end of a photocell D13, pin 2 of the op amp U7A is connected to a capacitor C71, and pin 3 of the chip U36 is connected to a +3V3 power supply. The end, one end of the resistor R65 and the other end of the photocell D13, the other end of the capacitor C71 and the other end of the resistor R65 are connected to the 4th pin of the operational amplifier U7A and the 6th pin of the operational amplifier U7B, the 7th pin of the operational amplifier U7B is connected to one end of the resistor R64 and one end of the resistor R67, the other end of the resistor R67 is connected to the VREF_1V25 power supply, the other end of the resistor R64 is connected to the 8th pin of the operational amplifier U7B and the 6th pin of the chip U36, and the models of the operational amplifier U7A and the operational amplifier U7B are LMC6482AIM; Pin 3 of the chip U33, pin 3 of the chip U34 and pin 3 of the chip U36 are connected to one end of a capacitor C72, one end of a capacitor C73, one end of a capacitor C74, one end of a resistor R66 and one end of a transistor Q14, the other end of the capacitor C72, the other end of the capacitor C73, the other end of the capacitor C74 and the other end of the transistor Q14 are grounded, and the other end of the resistor R66 is connected to a +3V3 power supply; The excitation light source driving unit includes a MOS tube Q11, a MOS tube Q12 and a MOS tube Q13, the models of the MOS tube Q11, the MOS tube Q12 and the MOS tube Q13 are NDC7002N, the 1st pin of the MOS tube Q11, the 1st pin of the MOS tube Q12 and the 1st pin of the MOS tube Q13 are connected to one end of a resistor R83, the other end of the resistor R83 is connected to one end of a capacitor C84 and the 4th pin of an operational amplifier U41, the model of the operational amplifier U41 is LMV321IDBVR, the 1st pin of the operational amplifier U41 is connected to one end of a capacitor 63 and one end of a resistor R82, the other end of the capacitor C63 is grounded, the other end of the resistor R82 is connected to one end of a capacitor C62, one end of a resistor R84 and one end of a resistor R81, the other end of the capacitor C62 and the other end of the resistor R84 are grounded, the other end of the resistor R81 is connected to the 15th pin of the chip U43, the other end of the capacitor C84 is connected to one end of a resistor R86, and the The other end of resistor R86 is connected to one end of resistor R87 and pin 2 of operational amplifier U41, pin 2 of MOS tube Q11, pin 2 of MOS tube Q12 and pin 2 of MOS tube Q13 are connected to one end of resistor R90 and the other end of resistor R87, the other end of resistor R90 is grounded, pin 3 of MOS tube Q11 is connected to one end of resistor R85 and pin 16 of chip U43, pin 3 of MOS tube Q12 is connected to one end of resistor R88 and pin 17 of chip U43, pin 3 of MOS tube Q13 is connected to one end of resistor R89 ​​and pin 18 of chip U43, the other end of resistor R85, the other end of resistor R88 and the other end of resistor R89 ​​are grounded, pin 6 of MOS tube Q11 is connected to pin 4 of light source LED1, pin 6 of MOS tube Q12 is connected to pin 3 of light source LED1, pin 6 of MOS tube Q13 is connected to pin 2 of light source LED1, and pin 1 of light source LED1 is connected to +5V0 power supply.

2. A PCR detection control system as claimed in claim 1, characterized in that: The cooling fan control unit includes a chip U30 and a chip U32, the models of the chip U30 and the chip U32 are TC1047AVNBTR, the pin 1 of the chip U30 is connected to one end of a capacitor C58 and connected to a +3V3 power supply, the other end of the capacitor C58 is grounded, the pin 3 of the chip U30 is connected to the pin 35 of the chip U29, the pin 1 of the chip U32 is connected to one end of a capacitor C62 and connected to a +3V3 power supply, the other end of the capacitor C62 is grounded, and the pin 3 of the chip U32 is connected to the pin 37 of the chip U29; The cooling fan control unit also includes a MOS tube Q5 and a MOS tube Q6, wherein the G pole of the MOS tube Q5 is connected to one end of a resistor R49 and pin 29 of a chip U29, the S pole of the MOS tube Q5 and the other end of the resistor R49 are grounded, the D pole of the MOS tube Q5 is connected to pin 3 of an interface P10, pin 2 of the interface P10 is connected to a VIN power supply, and pin 4 of the interface P10 is connected to pin 29 of the chip U29; the G pole of the MOS tube Q6 is connected to one end of a resistor R50 and pin 91 of the chip U29, the S pole of the MOS tube Q6 and the other end of the resistor R50 are grounded, the D pole of the MOS tube Q6 is connected to pin 3 of an interface P11, pin 2 of the interface P11 is connected to a VIN power supply, and pin 4 of the interface P11 is connected to pin 91 of the chip U29.

3. A PCR detection control system as claimed in claim 1, characterized in that: The stepper motor drive unit includes a chip U39, the model of the chip U39 is TMC2209, the pin 1 of the chip U39 is connected to the pin 1 of the interface P22, the pin 2 of the interface P22 is connected to the pin 26 of the chip U39, the pin 3 of the interface P22 is connected to the pin 24 of the chip U39, the pin 4 of the interface P22 is connected to the pin 21 of the chip U39, the interface P22 is used for an external motor, the capacitor C80 is connected between the pins 4 and 5 of the chip U39, the pin 6 of the chip U39 is connected to one end of the capacitor C81, the other end of the capacitor C81 is connected to one end of the capacitor C82 and one end of the capacitor C83, and is connected VCC_MO power supply, the other end of capacitor C82 and the other end of capacitor C83 are grounded, pin 8 of chip U39 is connected to one end of resistor R79 and one end of capacitor C79, the other end of capacitor C79 is grounded, the other end of resistor R79 is connected to one end of resistor R78 and pin 17 of chip U39, pins 7, 9 and 10 of chip U39 are connected to pin 15 of chip U39 and connected to +3V3 power supply, pin 23 of chip U39 is connected to one end of resistor R71, the other end of resistor R71 is grounded, pin 27 of chip U39 is connected to one end of resistor R72, the other end of resistor R72 is grounded; Pin 16 of the chip U39 is connected to pin 10 of interface P35, pin 19 of the chip U39 is connected to pin 9 of interface P35, pin 2 of the chip U39 is connected to pin 11 of interface P35, pin 14 of the chip U39 is connected to pin 8 of interface P35, pin 8 of interface P35 is connected to one end of resistor R09 and pin 46 of chip U43, the other end of resistor R09 is connected to pin 45 of chip U43, pin 9 of interface P35 is connected to pin 44 of chip U43, pin 10 of interface P35 is connected to pin 41 of chip U43, and pin 11 of interface P35 is connected to pin 38 of chip U43.

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