A centrifugal system, sample centrifugation method and liquid-based all-in-one machine

Abstract

The present application relates to the field of centrifugal technology, and specifically discloses a centrifugal system, a sample centrifugal method and a liquid-based all-in-one machine. The centrifugal system comprises a first bearing position, a second bearing position, a centrifuge and a conveying mechanism. The first bearing position is used for placing a centrifuge tube, and the centrifuge tube contains a sample mixture. The second bearing position is used for placing a balancing tube, and the liquid in the balancing tube is matched in mass with the sample mixture in the centrifuge tube. The centrifuge is provided with a containing position inside, which is used for containing the centrifuge tube or the centrifuge tube and the balancing tube. The conveying mechanism is used for transferring the centrifuge tube and the balancing tube from the first bearing position and the second bearing position to the containing position, so that the centrifuge can be balanced when performing centrifugal work. By setting the balancing tube, when the number of centrifuge tubes in the centrifuge does not reach the balance, the balancing tube can be added to balance the centrifuge when performing centrifugal work. Further, the separation effect is guaranteed, which is conducive to further obtaining a sample rich in cells.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of centrifugal technology, in particular to a centrifugal system, a sample centrifugal method and a liquid-based all-in-one machine. BACKGROUND

[0002] Before separating a liquid-based sample, the eluted and mixed sample needs to be transferred to a centrifugal tube containing gradient separation liquid for centrifugal treatment to obtain a sample liquid rich in sample cells. However, if the number of centrifugal tubes in the centrifuge does not reach a balanced state during centrifugal treatment, the separation effect is poor, which affects the effect of film making. SUMMARY

[0003] One of the purposes of the embodiments of the present application is to provide a centrifugal balancing method, which aims to at least solve the problem of poor separation effect and affecting film making effect when the number of centrifugal tubes in the existing centrifuge does not reach a balanced state.

[0004] To achieve the above-mentioned purpose, the technical solutions adopted by the embodiments of the present application are as follows:

[0005] A centrifugal system comprises:

[0006] A first bearing position for placing a centrifugal tube containing a sample mixture;

[0007] A second bearing position for placing a balancing tube containing a liquid different from the sample mixture, and the mass of the liquid in the balancing tube is adapted to the mass of the sample mixture in the centrifugal tube;

[0008] A centrifuge provided with a containing position for containing the centrifugal tube or the centrifugal tube and the balancing tube;

[0009] A conveying mechanism for transferring the centrifugal tube and the balancing tube from the first bearing position and the second bearing position to the containing position, respectively, so that the centrifuge achieves balance during centrifugal operation;

[0010] A bearing platform on which the conveying mechanism is installed;

[0011] A control system for controlling the operation of the conveying mechanism and the centrifuge, respectively.

[0012] Optionally, the centrifugal system further comprises a test tube rack and a conveying mechanism for conveying the test tube rack, and the first bearing position and the second bearing position are provided in the test tube rack.

[0013] Optionally, the centrifugal system further comprises a balance tube rack, a test tube rack and a conveying mechanism for conveying the test tube rack, the balance tube rack is installed on the bearing platform, the first bearing position is provided on the test tube rack, and the second bearing position is provided on the balance tube rack.

[0014] Optionally, the centrifuge further comprises a bearing seat, and the bearing seat is provided with a plurality of loading positions, and each loading position is used for placing one centrifugal tube or one balance tube.

[0015] Optionally, the conveying mechanism comprises a first grabbing mechanism, and the bearing seat is located in the containing position.

[0016] The first grabbing mechanism grabs the centrifugal tube or the balance tube and places it in the loading position, or the first grabbing mechanism takes out the centrifugal tube or the balance tube from each loading position.

[0017] Optionally, the conveying mechanism comprises a first grabbing mechanism and a second grabbing mechanism.

[0018] The first grabbing mechanism grabs the centrifugal tube or the balance tube and places it in the loading position, or the first grabbing mechanism takes out the centrifugal tube or the balance tube from each loading position.

[0019] The second grabbing mechanism is used for grabbing the bearing seat and placing the bearing seat in the containing position or taking out the bearing seat from the containing position.

[0020] Optionally, the centrifuge has a plurality of containing positions, and the plurality of containing positions are symmetrically arranged with respect to each other with the rotation axis of the centrifuge as a central axis.

[0021] Optionally, the centrifugal system further comprises a pipetting mechanism, and the pipetting mechanism is used for taking out or adding liquid to the centrifugal tube and / or the balance tube.

[0022] Optionally, the second grabbing mechanism comprises:

[0023] A guide structure extending in a straight line;

[0024] A grabbing structure comprising a first grabbing member and a second grabbing member connected to the guide structure;

[0025] The first grabbing member is used for transferring the bearing seat to the second grabbing member, or grabbing the bearing seat from the second grabbing mechanism and transferring;

[0026] The second grabbing member is used for grabbing the bearing seat from the first grabbing mechanism and transferring, or transferring the bearing seat to the first grabbing member.

[0027] Optionally, the centrifuge is provided with an opening and a storage area, and the centrifuge opening is provided with a switch door, and the switch door comprises:

[0028] A base assembly comprising two first guide assemblies, respectively located on opposite sides of the opening;

[0029] A cover plate slidably connected to the base assembly through the first guide assembly, the cover plate having an open position and a closed position;

[0030] Wherein the first guide assembly is a guide groove, and the cover plate is slidably connected to the guide groove, and the cover plate can be moved to the open position or the closed position along the guide groove;

[0031] The base assembly further comprises a sunken groove in communication with the guide groove;

[0032] The switch door further comprises a second guide assembly provided with an abutting inclined surface, which is used to guide the cover plate to descend from the guide groove to the sunken groove to close the opening when the cover plate slides from the open position to the closed position.

[0033] The second purpose of the embodiment of the present application is to provide a sample centrifugation method, which adopts the above-mentioned centrifugal system for balancing, comprising the following steps:

[0034] Obtaining information from the control system whether the centrifuge reaches the balance state;

[0035] When the control system receives the information that the centrifuge reaches the balance state, the centrifuge tube on the first bearing position is transferred to the containing position, and the centrifuge is started for the first time;

[0036] When the control system receives the information that the centrifuge does not reach the balance state, the centrifuge tube on the first bearing position is transferred to the containing position, and the balancing tube on the second bearing position is transferred to the empty containing position in the centrifuge until the centrifuge reaches the balance state, and then the centrifuge is started for the first time;

[0037] When the first centrifugation is completed, part of the liquid in the centrifuge tube or the balancing tube is taken out.

[0038] Optionally, the centrifugal system further comprises a bearing seat, a first grabbing mechanism and a second grabbing mechanism;

[0039] The transferring of the centrifuge tube in the first bearing position or the centrifuge tube in the first bearing position and the balancing tube in the second bearing position to the containing position comprises the following steps:

[0040] transferring the centrifuge tube in the first carrying position or the centrifuge tube in the first carrying position and the balance tube in the second carrying position into the carrying seat by using the first grabbing mechanism;

[0041] and transferring the carrying seat containing the centrifuge tube or the centrifuge tube and the balance tube into the containing position by using the second grabbing mechanism.

[0042] Optionally, when the first centrifugation is finished, part of the liquid in the centrifuge tube or the balance tube in the centrifuge is taken out, and the centrifuge is kept in a balanced state, and the method further comprises the steps of starting the centrifuge for second centrifugation, and taking out the centrifuge tube or the balance tube processed by the second centrifugation from the loading position.

[0043] Optionally, the method further comprises the step of taking out the sample mixture in the centrifuge tube in the centrifuge after the second centrifugation.

[0044] Optionally, the carrying seat placed in the containing position is taken out of the centrifuge by using the second grabbing mechanism, and the centrifuge tube or the balance tube is taken out of the carrying seat by using the first grabbing mechanism; or the centrifuge tube or the balance tube is taken out of the centrifuge by using the first grabbing mechanism.

[0045] Optionally, the centrifuge tube is placed on a test tube rack after being taken out, and the balance tube is placed on the balance tube rack after being taken out; or,

[0046] the centrifuge tube is placed on a test tube rack after being taken out, and the balance tube is placed on the test tube rack after being taken out.

[0047] A third object of the embodiments of the present application is to provide a liquid-based integrated machine comprising the centrifuge system described above; the liquid-based integrated machine performs centrifugation processing according to the sample centrifugation method described above.

[0048] Compared with the prior art, the present application has the following beneficial effects:

[0049] The centrifugal system, the sample centrifugation method and the liquid-based integrated machine provided by the application, the centrifugal system comprises a first bearing position, a second bearing position, a centrifuge and a conveying mechanism, the first bearing position is used for placing a centrifugal tube, and the centrifugal tube contains a sample mixed solution. The second bearing position is used for placing a balancing tube, and the balancing tube contains a liquid different from the sample mixed solution, and the mass of the liquid in the balancing tube is matched with the mass of the sample mixed solution in the centrifugal tube. The centrifuge is provided with a containing position, the containing position is used for containing the centrifugal tube or the centrifugal tube and the balancing tube. The conveying mechanism is used for transferring the centrifugal tube and the balancing tube from the first bearing position and the second bearing position to the containing position, so that the centrifuge realizes balance when performing centrifugation. The application adds the balancing tube, so that the centrifuge realizes balance when performing centrifugation when the number of the centrifugal tubes in the centrifuge does not reach balance. Further, the separation effect is ensured, which is beneficial to further obtain a sample rich in cells and facilitate medical examination in the later stage. BRIEF DESCRIPTION OF DRAWINGS

[0050] In order to more clearly illustrate the technical solutions of the embodiments of the application, the following will briefly introduce the drawings needed to be used in the embodiment description. Obviously, the drawings in the following description are some embodiments of the application, and other drawings can also be obtained by those skilled in the art without creative labor.

[0051] Figure 1 is a plane schematic view of the centrifugal system provided by the first embodiment of the application;

[0052] Figure 2 is Figure 1 is a local enlarged schematic view of A in FIG. 1;

[0053] Figure 3 is Figure 1 is a local enlarged schematic view of B in FIG. 1;

[0054] Figure 4 is a three-dimensional structural schematic view of the test tube rack provided by the first embodiment of the application;

[0055] Figure 5 is a three-dimensional structural schematic view of the centrifuge provided by the first embodiment of the application with a second grabbing mechanism;

[0056] Figure 6 is a three-dimensional structural schematic view of the second grabbing mechanism provided by the first embodiment of the application;

[0057] Figure 7 is a three-dimensional structural schematic view of the centrifuge provided by the first embodiment of the application with a switch door;

[0058] Figure 8 is a three-dimensional structural schematic view of the switch door provided by the first embodiment of the application;

[0059] Figure 9 This is a three-dimensional structural diagram of a portion of the opening and closing doors provided in Embodiment 1 of the present invention;

[0060] Figure 10 This is a three-dimensional structural diagram of the conveying mechanism provided in Embodiment 1 of the present invention;

[0061] Figure 11 This is a plan view of the centrifugation system provided in Embodiment 2 of the present invention;

[0062] Figure 12 yes Figure 11 A magnified view of a portion of point C in the middle;

[0063] Figure 13 This is a plan view of the liquid-based integrated machine provided in Embodiment 4 of the present invention.

[0064] Figure label:

[0065] 10. Centrifugal system; 11. First bearing position; 12. Second bearing position;

[0066] 13. Centrifuge; 131. Containing position; 132. Support; 1321. Loading position; 133. Opening; 134. Storage area;

[0067] 14. Transport mechanism; 141. First gripping mechanism; 142. Second gripping mechanism; 1421. Guide structure; 1422. Gripping structure; 1401. First gripping component; 1402. Second gripping component;

[0068] 15. Supporting platform; 151. Sample loading area; 152. Transport area; 153. Sample unloading area;

[0069] 16. Test tube rack; 161. Receiving hole; 17. Tilting drive mechanism; 18. Centrifuge tube; 19. Balancing tube;

[0070] 20. Pipette mechanism; 201. Liquid dispensing mechanism; 202. Aspiration mechanism; 203. Sample dispensing mechanism;

[0071] 21. Opening / closing door; 211. Base assembly; 2111. First guide assembly; 2101. Guide groove; 2112. Second guide assembly; 212. Cover plate; 213. Sealing frame; 214. Abutting slope; 215. Recessed groove;

[0072] 22. Install pipe supports;

[0073] 100. Liquid-based integrated machine; 1001. Washing and mixing device; 1002. Centrifugation device; 1003. Sedimentation and dyeing device; 1004. Immersion treatment device. Detailed Implementation

[0074] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are some of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by those of ordinary skill in the art without creative effort should fall within the scope of the present application.

[0075] Embodiment one:

[0076] Please refer to Figures 1 to 10The centrifugal system 10 provided by the embodiment comprises a first bearing position 11, a second bearing position 12, a centrifuge 13, a conveying mechanism 14, a bearing platform 15 and a control system (not shown in the figure). The first bearing position 11 is used for placing a centrifugal tube 18, and the centrifugal tube 18 contains a sample mixture. The second bearing position 12 is used for placing a balancing tube 19, and the balancing tube 19 contains a liquid different from the sample mixture, and the mass of the liquid in the balancing tube 19 is adapted to the mass of the sample mixture in the centrifugal tube 18. The centrifuge 13 is internally provided with a containing position 131, and the containing position 131 is used for containing the centrifugal tube 18 or for containing the centrifugal tube 18 and the balancing tube 19. The conveying mechanism 14 is used for transferring the centrifugal tube 18 and the balancing tube 19 from the first bearing position 11 and the second bearing position 12 to the containing position 131, so that the centrifuge 13 can be balanced when performing centrifugal work. The conveying mechanism 14 is installed on the bearing platform 15. The control system is used for controlling the work of the conveying mechanism 14 and the centrifuge 13 respectively. Exemplarily, the centrifuge 13 provided by the application relies on the centrifugal force of rotation to separate the sample in the centrifugal tube 18 when working, and the supernatant in the upper layer is removed, so that a sample liquid rich in cells is obtained. However, in a liquid-based preparation device with high automation, batch centrifugal processing is performed, and sometimes the number of centrifugal tubes 18 is insufficient to balance the centrifuge 13. If the centrifugal processing is performed in an unbalanced state, the centrifuge 13 will be damaged. Therefore, the centrifugal system 10 provided by the application is provided with the balancing tube 19, and when the number of centrifugal tubes 18 in the centrifuge 13 does not reach a balanced state, the balancing tube 19 is added to balance the centrifuge 13 when performing centrifugal work. Further, the separation effect is ensured, which is beneficial to further obtain a sample and a sample sheet with high quality, and is beneficial to medical examination in the later period. When the number of centrifugal tubes 18 is sufficient to balance the centrifuge 13, the balancing tube 19 is not needed. In addition, it should be noted that the centrifugal tube 18 and the balancing tube 19 have no difference in structure, and the centrifugal tube 18 and the balancing tube 19 are actually the same kind of test tube. After the sample and the gradient separation liquid are mixed in the empty test tube to form a sample mixture, centrifugal processing is needed, which is the centrifugal tube 18. When the gradient separation liquid is only added to the empty test tube, the empty test tube is used for balancing, which is the balancing tube 19. The first bearing position 11 and the second bearing position 12 also have no difference in structure, and the first bearing position 11 and the second bearing position 12 are containing cavities used for placing the centrifugal tube 18 or the balancing tube 19. In addition, the gradient separation liquid in the balancing tube 19 should be adapted to the mass of the sample mixture in the centrifugal tube 18. Here, the adaptation means that the mass of the two is within a certain error range. Further, the preferred solution is that the mass of the two is equal, and at this time, the separation effect of the sample mixture is the best.

[0077] Please refer to Figure 1 、 Figure 2 、 Figure 4As an implementation, the centrifugal system 10 further comprises a test tube rack 16 and a conveying mechanism (not shown in the figure) for conveying the test tube rack 16, and the first carrying position 11 and the second carrying position 12 are both arranged on the test tube rack 16. Exemplarily, the test tube rack 16 is used for carrying test tubes. The conveying mechanism comprises a conveying mechanism for conveying the test tube rack 16 with test tubes. It should be noted that the positions of the first carrying position 11 and the second carrying position 12 are not fixed, and a plurality of accommodation holes 161 for placing test tubes are arranged on the test tube rack 16, and the first carrying position 11 is formed when a centrifugal tube 18 is placed in the accommodation hole 161, and the second carrying position 12 is formed when a leveling tube 19 is placed in the accommodation hole 161. The test tube rack 16 provided in the embodiment comprises four accommodation holes 161 and can be used for placing four test tubes. Of course, in specific applications, the number of accommodation holes 161 is not limited to four, for example, as an alternative, the number of accommodation holes 161 can be three or five.

[0078] Please refer to Figure 1 , Figure 2 , Figure 4 As an implementation, the carrying platform 15 comprises a sample feeding area 151, a conveying area 152 and a sample discharging area 153, and the sample feeding area 151, the conveying area 152 and the sample discharging area 153 are arranged in a U shape. The test tube rack 16 carries empty test tubes from the sample feeding area 151, the test tubes in the test tube rack 16 are transferred from the conveying area 152 to the centrifuge 13 for centrifugal processing, and after the centrifugal processing is completed, the test tubes are placed back into the test tube rack 16 located in the conveying area 152, and then buffer solution is added into the centrifugal tube 18 to mix with the sample to form sample liquid, and the sample liquid is transferred to the sedimentation tube for sedimentation processing. The test tube rack 16 and the test tubes on the test tube rack 16 are discharged through the sample discharging area 153.

[0079] Please refer to Figure 4 , Figure 5 , Figure 6 , Figure 12 As an implementation, the centrifuge 13 further comprises a carrying seat 132, and the carrying seat 132 is provided with a plurality of loading positions 1321, and each loading position 1321 is used for placing a centrifugal tube 18 or a leveling tube 19. Exemplarily, the carrying seat 132 is used for being placed in the centrifuge 13, and the centrifugal tube 18 or the leveling tube 19 is placed in the centrifuge 13 for centrifugal processing through the carrying seat 132. It should be noted that the carrying seat 132 provided in the embodiment comprises four loading positions 1321, and one carrying seat 132 can place four test tubes. Of course, in specific applications, the number of loading positions 1321 is not limited to four, for example, as an alternative, the number of loading positions 1321 can be three or five.

[0080] Please refer to Figure 4 , Figure 5 , Figure 6 , Figure 12As an embodiment, the conveying mechanism 14 comprises a first grabbing mechanism 141 and a second grabbing mechanism 142. The first grabbing mechanism 141 grabs the centrifuge tube 18 or the balancing tube 19 and places it into the loading position 1321, or the first grabbing mechanism 141 takes the centrifuge tube 18 or the balancing tube 19 from each loading position 1321. The second grabbing mechanism 142 is used to grab the carrier 132 and place it into the containing position 131 or take it out from the containing position 131. The first grabbing mechanism 141 grabs the centrifuge tube 18 or the balancing tube 19 from the test tube rack 16 located in the conveying area 152 and places it into the loading position 1321, and it is necessary to note that at this time the carrier 132 is placed on the second grabbing mechanism 142, and the second grabbing mechanism 142 places the carrier 132 carrying the centrifuge tube 18 or the balancing tube 19 on the containing position 131. Conversely, the second grabbing mechanism 142 takes the carrier 132 carrying the centrifuge tube 18 or the balancing tube 19 from the containing position 131, and the first grabbing mechanism 141 grabs the centrifuge tube 18 or the balancing tube 19 on the carrier 132 of the second grabbing mechanism 142 and places it into the test tube rack 16 located in the conveying area 152.

[0081] Please refer to Figure 4 , Figure 5 , Figure 6 , Figure 12 As an embodiment, the centrifuge 13 has a plurality of containing positions 131, and the plurality of containing positions 131 are symmetrically arranged with respect to the rotation axis of the centrifuge 13 as the central axis. Exemplarily, the centrifuge 13 of the present embodiment has four containing positions 131, and one containing position 131 is used to place one carrier 132, and one carrier 132 can be used to place two centrifuge tubes 18 or balancing tubes 19, that is, when the centrifuge 13 is fully loaded, eight centrifuge tubes 18 or balancing tubes 19 should be placed in the centrifuge 13. Of course, in specific applications, the centrifuge 13 does not need to be fully loaded to perform centrifugal processing. It is only necessary that the number of centrifuge tubes 18 and balancing tubes 19 in the centrifuge 13 is an integer multiple of the number of containing positions 131, that is, the centrifuge 13 can be balanced. For example, if there are three centrifuge tubes 18, each centrifuge tube 18 is placed on the carrier 132 of the centrifuge 13, and the remaining carrier 132 without the centrifuge tube 18 is placed with a balancing tube 19. If there is one centrifuge tube 18, the centrifuge tube 18 is placed on the carrier 132 of the centrifuge 13, and the remaining three carriers 132 without the centrifuge tube 18 are respectively placed with a balancing tube 19. If there are five centrifuge tubes 18, two centrifuge tubes 18 are placed on one carrier 132, and the remaining three carriers 132 are respectively placed with one centrifuge tube 18, and one balancing tube 19 is respectively placed on the three carriers 132 with one centrifuge tube 18. Of course, in specific applications, the number of containing positions 131 is not limited to four, and can also be six or two, and the present application does not make too many limitations on this.

[0082] Referring to Figure 3 , Figure 4 , Figure 10 As an embodiment, the centrifugal system 10 further comprises a pipetting mechanism 20 for taking out or adding liquid to the centrifugal tube 18 and / or the balancing tube 19. Exemplarily, the pipetting mechanism 20 comprises a liquid adding mechanism 201, a sample adding mechanism 203 and a suction mechanism 202. The liquid adding mechanism 201 is arranged beside the conveying area 152, the sample adding mechanism 203 is used for adding sample to the test tube of the test tube rack 16, and the liquid adding mechanism 201 is used for adding gradient separation liquid to the test tube of the test tube rack 16. The suction mechanism 202 is used for sucking the supernatant after the first centrifugation and the second centrifugation. It should be noted that the gradient separation liquid is added to the empty test tube first, and then the sample is added. In addition, the centrifugal system 10 further comprises a tilting driving mechanism 17. The empty test tube is in a vertical state when the gradient separation liquid is added, and then the tilting driving mechanism 17 drives the test tube rack 16 to tilt so that the test tube is tilted to a preset angle, and then the sample is added to the test tube. The purpose of arranging the tilting driving mechanism 17 is to make the centrifugal tube 18 in a tilted state when the sample is added, so that the sample can slowly enter the gradient separation liquid along the wall of the centrifugal tube 18, so that the sample has enough contact time with the gradient separation liquid, thereby achieving good separation effect of the sample. It should be noted that the centrifuge 13 of the present application needs to be centrifuged twice, and the supernatant needs to be sucked by the suction mechanism 202 after each centrifugation.

[0083] Referring to Figure 5 , Figure 6As an implementation form, the second grabbing mechanism 142 comprises a guide structure 1421 and a grabbing structure 1422. The guide structure 1421 extends in a straight line; the grabbing structure 1422 comprises a first grabbing component 1401 and a second grabbing component 1402 connected to the guide structure 1421. The first grabbing component 1401 is used to transfer the carrier 132 to the second grabbing component 1402 or to grab and transfer the carrier 132 from the second grabbing mechanism 142. The second grabbing component 1402 is used to grab and transfer the carrier 132 from the first grabbing mechanism 141 or to transfer the carrier 132 to the first grabbing component 1401. The second grabbing mechanism 142 provided by the application can relay the transfer of the carrier 132 through the first grabbing component 1401 and the second grabbing component 1402 and the guide structure 1421 extending in a straight line. The first grabbing component 1401 can directly grab and transfer the carrier 132 from the second grabbing component 1402, and the second grabbing component 1402 can also directly grab and transfer the carrier 132 from the first grabbing component 1401. The carrier 132 does not need to be placed in a preset position, and the transfer of the carrier 132 in the air is directly realized. The moving track of the carrier 132 extends in a straight line along the guide structure 1421, thereby avoiding mechanical interference in the limited space of the small machine. Moreover, the first grabbing component 1401 and the second grabbing component 1402 simultaneously move to transfer and load the carrier 132 of the sample container, thereby solving the problems of large stroke, long time and low work efficiency of a single mechanical hand.

[0084] Please refer to Figure 5 , Figure 7 , Figure 8 , Figure 10 As an implementation form, the centrifuge 13 is provided with an opening 133 and a storage area 134. The centrifuge 13 is provided with a switch door 21 at the opening 133. The switch door 21 comprises a base assembly 211 and a cover plate 212. The base assembly 211 comprises two first guide assemblies 2111, and the two first guide assemblies 2111 are respectively located at opposite sides of the opening 133. The cover plate 212 is slidably connected to the base assembly 211 through the first guide assemblies 2111. The cover plate 212 has an open position and a closed position. The first guide assembly 2111 is a guide groove 2101, and the cover plate 212 is slidably connected to the guide groove 2101. The cover plate 212 can move to the open position or the closed position along the guide groove 2101. After the opening 133 is closed, the sound generated inside the centrifuge 13 during the start of the centrifuge 13 can be prevented from being transmitted outside, thereby reducing the noise. When the cover plate 212 slides from the closed position to the open position, the opening 133 is opened, so that the centrifuge tube 18 in the storage area 134 or the carrier 132 for storing the centrifuge tube 18 can be normally taken out or placed. Through the arrangement of the cover plate 212, the noise reduction effect can be achieved without affecting the normal operation of the centrifuge 13.

[0085] Please refer to Figure 5、 Figure 7 、 Figure 8 、 Figure 10 As an implementation form, the switch door 21 further comprises a sealing frame 213, the sealing frame 213 is arranged around the opening 133, and the sealing frame 213 is used to be attached to the cover plate 212 to seal the opening 133 when the cover plate 212 moves to the closed position; wherein at least the contact part of the cover plate 212 is made of elastic material. In the embodiment, the cover plate 212 can be made of hard material such as metal or plastic, and the sealing performance of directly covering the opening 133 is weak, so the sealing frame 213 with elastic structure is added to improve the sealing performance of the cover plate 212 covering the opening 133, improve the noise reduction effect, and the sealing frame 213 arranged around the opening 133 can increase the height, facilitate the attachment of the cover plate 212 to seal the opening 133. Exemplarily, the sealing frame 213 is made of elastic material as a whole, so that the sealing effect of the cover plate 212 covering the opening 133 is good. Of course, in specific applications, the sealing frame 213 can also be partially made of elastic material, specifically, the sealing frame 213 comprises a frame body and an elastic body, the frame body can be made of metal or plastic material, which is convenient for installation, and the elastic body is made of elastic material, which can be extruded by the cover plate 212 to play a sealing role.

[0086] Please refer to Figure 5 、 Figure 7 、 Figure 8 、 Figure 10 As an implementation form, the base assembly 211 further comprises a sunken groove 215 in communication with the guide groove 2101; the switch door 21 further comprises a second guide assembly 2112 provided with an abutting inclined surface 214, the abutting inclined surface 214 is used to guide the cover plate 212 to descend from the guide groove 2101 to the sunken groove 215 when the cover plate 212 slides from the open position to the closed position, so as to close the opening 133. In addition, when the switch door 21 is provided with the sealing frame 213, if the cover plate 212 directly translates to cover the opening 133, a large friction force will be generated with the sealing frame 213, affecting the movement of the cover plate 212, and the cover plate 212 is easy to be damaged. By arranging the guide structure 1421 to guide the cover plate 212 to descend when the cover plate 212 slides to the closed position, the friction between the cover plate 212 and the sealing frame 213 before the cover plate 212 moves to the closed position is reduced, the cover plate 212 moves smoothly, and the cover plate 212 is prevented from being damaged. When the cover plate 212 starts to slide from the closed position to the open position, the cover plate 212 directly translates with a large friction force because the cover plate 212 is close to the sealing frame 213 at this time, and the guide structure 1421 is used to guide the cover plate 212 to ascend, so that the cover plate 212 is lifted and then moved, the friction is reduced, and the cover plate 212 slides smoothly.

[0087] Please refer to Figures 1 to 10 The second purpose of the embodiment of the present application is to provide a sample centrifugation method, which is balanced by using the above-mentioned centrifugal system 10, comprising the following steps:

[0088] S1, the control system acquires information on whether the centrifuge 13 has reached equilibrium; it should be noted that whether the centrifuge 13 has reached equilibrium is not determined at the step of centrifugal processing, but at the previous step of elution mixing, information on whether the sample needs to be balanced and how to balance during centrifugal processing is acquired.

[0089] S2, when the control system receives information that the centrifuge 13 has reached equilibrium, the centrifuge tube 18 on the first bearing position 11 is transferred to the containing position 131, and the centrifuge 13 is started for the first centrifugation.

[0090] S3, when the control system receives information that the centrifuge 13 has not reached equilibrium, the centrifuge tube 18 on the first bearing position 11 is transferred to the containing position 131, and the balancing tube 19 on the second bearing position 12 is transferred to the empty containing position 131 in the centrifuge 13 until the centrifuge 13 reaches equilibrium, and then the centrifuge 13 is started for the first centrifugation.

[0091] S4, when the first centrifugation is completed, part of the liquid in the centrifuge tube 18 or the balancing tube 19 in the centrifuge 13 is removed. In this step, the supernatant in the centrifuge tube 18 or the gradient separation liquid in the balancing tube 19 is removed by the suction mechanism 202. The supernatant is the waste liquid containing fewer sample cells after centrifugal processing.

[0092] As an embodiment, the centrifugal system 10 further comprises a bearing seat 132, a first grabbing mechanism 141 and a second grabbing mechanism 142; transferring the centrifuge tube 18 in the first bearing position 11 or the centrifuge tube 18 in the first bearing position 11 and the balancing tube 19 in the second bearing position 12 to the containing position 131 comprises the following steps:

[0093] S31, the first grabbing mechanism 141 is used to transfer the centrifuge tube 18 in the first bearing position 11 or the centrifuge tube 18 in the first bearing position 11 and the balancing tube 19 in the second bearing position 12 to the bearing seat 132;

[0094] S311, when balancing is needed, the first grabbing mechanism 141 grabs the centrifuge tube 18 in the first bearing position 11 and the balancing tube 19 in the second bearing position 12 and places them in the bearing seat 132;

[0095] S312, when balancing is not needed, the first grabbing mechanism 141 grabs the centrifuge tube 18 in the first bearing position 11 and places it in the bearing seat 132.

[0096] S32, the second grabbing mechanism 142 is used to transfer the bearing seat 132 containing the centrifuge tube 18 or the balancing tube 19 to the containing position 131.

[0097] As an implementation, when the first centrifugation is finished, part of the liquid in the centrifuge tube 18 or the balancing tube 19 in the centrifuge 13 is taken out, and the centrifuge 13 is kept in a balanced state, further comprising:

[0098] S5, starting the centrifuge 13 for the second centrifugation, and taking the centrifuge tube 18 or the balancing tube 19 after the second centrifugation from the loading position 1321. It should be noted that the liquid mass of each centrifuge tube 18 or balancing tube 19 in the centrifuge 13 is the same after the first centrifugation, so that the centrifuge 13 is still in a balanced state during the second centrifugation.

[0099] As an implementation, after the second centrifugation, the following step is further included: S6, taking out the sample mixture in the centrifuge tube 18 in the centrifuge 13. After the second centrifugation, the suction mechanism 202 first sucks the supernatant in the centrifuge tube 18, so that the sample liquid remaining in the centrifuge tube 18 is rich in cell samples.

[0100] S71, using the second grabbing mechanism 142 to take out the carrier 132 placed in the containing position 131 from the centrifuge 13, and using the first grabbing mechanism 141 to take out the centrifuge tube 18 or the balancing tube 19 from the carrier 132. If it is normal centrifugation, only the centrifuge tube 18 needs to be taken out, and if it is balancing centrifugation, the centrifuge tube 18 and the balancing tube 19 need to be taken out. In any case, after the second centrifugation is completed, the test tubes in the centrifuge 13 are taken out and placed back in the test tube rack 16.

[0101] S81, after the centrifuge tube 18 is taken out, it is placed in the test tube rack 16, and after the balancing tube 19 is taken out, it is placed in the test tube rack 16. It should be noted that the centrifuge tube 18 and the balancing tube 19 can be placed back in the original test tube rack 16, or placed in a new empty test tube rack 16.

[0102] Example Two:

[0103] Please refer to Figure 11 、 Figure 12 The difference between this embodiment and Example One is mainly that the placement position of the balancing tube 19 is different. Specifically embodied in:

[0104] As an embodiment, the centrifugal system 10 further comprises a balancing tube rack 22, a test tube rack 16, and a conveying mechanism for conveying the test tube rack 16, the balancing tube rack 22 is installed on the bearing platform 15, the first bearing position 11 is provided for the test tube rack 16, and the second bearing position 12 is provided for the balancing tube rack 22. The difference between this embodiment and the first embodiment is that, in the first embodiment, the test tube rack 16 can simultaneously place the centrifugal tube 18 and the balancing tube 19, while in this embodiment, the test tube rack 16 only places the centrifugal tube 18. The balancing tube rack 22 is separately provided to place the balancing tube 19. Such a structural design can make the balancing tube 19 reusable, and after balancing, it can be placed back in the balancing tube rack 22 for continuous use next time.

[0105] Different from the first embodiment, after the second centrifugation is completed, the balancing tube 19 is taken out, and at this time, the steps are as follows:

[0106] S82, after the centrifugal tube 18 is taken out and placed in the test tube rack 16, the balancing tube 19 is taken out and placed in the balancing tube rack 22; of course, if the balancing tube 19 is no longer used, it needs to be discarded, and it can also be placed in the test tube rack 16 and removed through the sample outlet area 153.

[0107] In addition to the above differences, the structure of the centrifugal system and the centrifugal method of the sample provided by this embodiment can be optimally designed with reference to the first embodiment, and will not be described in detail here.

[0108] Embodiment three:

[0109] The difference between this embodiment and the first embodiment mainly lies in the structure of the grabbing mechanism of the centrifugal tube 18 or the balancing tube 19 placed in the bearing seat 132. Specifically embodied in: this embodiment only has one grabbing mechanism, and the specific structure of the grabbing mechanism of this embodiment is referred to the first embodiment.

[0110] As an embodiment, the conveying mechanism 14 comprises a first grabbing mechanism 141, and the bearing seat 132 is located in the containing position 131. The first grabbing mechanism 141 grabs the centrifugal tube 18 or the balancing tube 19 and places it in the loading position 1321, or the first grabbing mechanism 141 takes out the centrifugal tube 18 or the balancing tube 19 from each loading position 1321. In the first embodiment, two grabbing mechanisms are provided, i.e. the first grabbing mechanism 141 and the second grabbing mechanism 142, the first grabbing mechanism 141 first grabs the centrifugal tube 18 or the balancing tube 19 and places it in the second grabbing mechanism 142, and the second grabbing mechanism 142 places the centrifugal tube 18 or the balancing tube 19 on the bearing seat 132 in the centrifuge 13, while in this embodiment, the centrifugal tube 18 or the balancing tube 19 is directly placed on the bearing seat 132 in the centrifuge 13 through the first grabbing mechanism 141.

[0111] Different from the first embodiment, after the second centrifugation is completed, the centrifugal tube 18 or the balancing tube 19 needs to be taken out, and at this time, the steps are as follows:

[0112] S72, the centrifugal tube 18 or the balancing tube 19 is taken out from the centrifuge 13 by the first grabbing mechanism 141. If it is normal centrifugation, the first grabbing mechanism 141 only needs to grab the centrifugal tube 18, and if it is centrifugation after balancing, the first grabbing mechanism 141 needs to grab the centrifugal tube 18 and the balancing tube 19.

[0113] In addition to the above differences, the structure of the centrifugal system and the sample centrifugation method provided by the embodiment can be optimized and designed according to Embodiment 1, which will not be described in detail here.

[0114] Embodiment Four:

[0115] Please refer to Figure 13 The main difference between the embodiment and Embodiment 1 is that the protected subject is different.

[0116] The third purpose of the embodiment of the application is to provide a liquid-based integrated machine 100, which comprises the above-mentioned centrifugal system 10; the liquid-based integrated machine 100 is subjected to centrifugal treatment according to the above-mentioned sample centrifugation method. Exemplarily, the liquid-based integrated machine 100 provided by the application is used for liquid-based cell slice preparation, and the liquid-based integrated machine 100 comprises an elution and mixing device 1001, a centrifugal device 1002, a sedimentation and staining device 1003, and an immersion treatment device 1004. The elution and mixing device 1001 is used for elution and mixing of the sample in the sample container; the centrifugal device 1002 is used for centrifugation of the eluted and mixed sample; the sedimentation and staining device 1003 is used for sedimentation and staining treatment of the centrifuged sample; and the immersion treatment device 1004 is used for immersion treatment of the stained sample. The liquid-based integrated machine 100 further comprises a bearing platform 15, and the elution and mixing device 1001, the centrifugal device 1002, the sedimentation and staining device 1003, and the immersion treatment device 1004 are all arranged on the bearing platform 15. The liquid-based slice preparation equipment provided by the application can automatically realize the whole process of slice preparation, including but not limited to sample feeding, sample elution and mixing, reagent addition, centrifugal treatment, sedimentation and staining, immersion treatment, and output of slides. The centrifugal system 10 of the application is applied to the centrifugal device 1002, and the centrifugal device 1002 uses the above-mentioned centrifugation method to centrifuge the sample, thereby ensuring the separation effect and being conducive to further obtaining a sample and a slice with high quality, which is convenient for medical examination in the later stage.

[0117] In addition to the above differences, the structure of the centrifugal system and the sample centrifugation method provided by the embodiment can be optimized and designed according to Embodiment 1 to Embodiment 3, which will not be described in detail here.

[0118] The above merely provides a specific implementation of the present application, but the protection scope of the present application is not limited thereto, and any skilled person in the art can easily think of various equivalent modifications or replacements within the technical range disclosed by the present application, and these modifications or replacements should be covered within the protection scope of the present application. Therefore, the protection scope of the present application should be subject to the protection scope of the claims.

Claims

1. A centrifuge system, characterized in that, include: A first bearing position is used to place a centrifuge tube containing a sample mixture. The second bearing position is used to place the balancing tube, which contains a liquid that is different from the sample mixture, and the mass of the liquid in the balancing tube is matched with the mass of the sample mixture in the centrifuge tube. A centrifuge, wherein the centrifuge has a receiving position for holding the centrifuge tubes, or for holding the centrifuge tubes and the balancing tubes; A conveying mechanism is used to transfer the centrifuge tube and the balancing tube from the first bearing position and the second bearing position to the receiving position, respectively, so that the centrifuge can achieve balance during centrifugation. The carrying platform, on which the transport mechanism is mounted; A control system, wherein the control system is used to control the operation of the conveying mechanism and the centrifuge respectively; The centrifuge further includes a support base, and the conveying mechanism includes a second gripping mechanism, which includes: The guide structure extends along a straight line; The gripping structure includes a first gripping member and a second gripping member connected to the guide structure; The first gripping member is used to transfer the carrier to the second gripping member, or to grip the carrier from the second gripping member and transfer it, and to make the movement trajectory of the carrier extend in a straight line along the guide structure; The second gripping member is used to grip the carrier from the first gripping member and transfer it, or to transfer the carrier to the first gripping member.

2. The centrifuge system as described in claim 1, characterized in that, The centrifugation system also includes a test tube rack and a conveying mechanism for transporting the test tube rack, wherein the first bearing position and the second bearing position are both located on the test tube rack.

3. The centrifuge system as described in claim 1, characterized in that, The centrifugation system also includes a balancing tube rack, a test tube rack, and a conveying mechanism for conveying the test tube rack. The balancing tube rack is installed on the bearing platform, with the first bearing position located on the test tube rack and the second bearing position located on the balancing tube rack.

4. The centrifuge system according to any one of claims 1-3, characterized in that, The support is provided with a number of loading positions, each of which is used to place one of the centrifuge tubes or one of the balancing tubes.

5. The centrifuge system as described in claim 4, characterized in that, The transport mechanism includes a first gripping mechanism, and the carrier is located in the receiving position; The first gripping mechanism grips the centrifuge tube or the balancing tube and places it into the loading position, or the first gripping mechanism removes the centrifuge tube or the balancing tube from each of the loading positions.

6. The centrifuge system as described in claim 4, characterized in that, The conveying mechanism includes a first gripping mechanism, which grips the centrifuge tube or the balancing tube and places it into the loading position, or the first gripping mechanism removes the centrifuge tube or the balancing tube from each of the loading positions; The second gripping mechanism is used to grip the carrier and place or remove the carrier into the receiving position.

7. The centrifuge system according to any one of claims 1-3, characterized in that, The centrifuge has several receiving positions, and the several receiving positions are arranged symmetrically in pairs with the centrifuge's rotation axis as the central axis.

8. The centrifuge system according to any one of claims 1-3, characterized in that, The centrifugation system further includes a pipetting mechanism for removing liquid from the centrifuge tube and / or the balancing tube or adding liquid to the centrifuge tube and / or the balancing tube.

9. The centrifuge system according to any one of claims 1-3, characterized in that, The centrifuge has an opening and a storage area. The opening of the centrifuge is equipped with a switch door, which includes: A base assembly, the base assembly including two first guide components, the two first guide components being located on opposite sides of the opening; A cover plate, slidably connected to the base assembly via the first guide assembly, the cover plate having an open position and a closed position; The first guide component is a guide groove, and the cover plate is slidably connected to the guide groove. The cover plate can move along the guide groove to the open position or the closed position. The base assembly also includes a recessed groove that communicates with the guide groove; The door also includes a second guide assembly, which has an abutting slope. The abutting slope is used to guide the cover plate down from the guide groove to the sinking groove when the cover plate slides from the open position to the closed position, so as to close the opening.

10. A sample centrifugation method, characterized in that, Balancing using the centrifuge system according to any one of claims 1 to 9 includes the following steps: The control system obtains information on whether the centrifuge has reached a balance. When the control system receives information that the centrifuge has reached a balanced state, it transfers the centrifuge tube on the first bearing position to the receiving position and starts the centrifuge for the first centrifugation. When the control system receives information that the centrifuge has not reached a balanced state, it transfers the centrifuge tube on the first bearing position to the receiving position, and transfers the balancing tube on the second bearing position to the empty receiving position in the centrifuge, until the centrifuge reaches a balanced state, and then starts the centrifuge for the first centrifugation. At the end of the first centrifugation, a portion of the liquid in the centrifuge tube or the centrifuge tube and the balancing tube is removed.

11. The sample centrifugation method as described in claim 10, characterized in that, The centrifugation system also includes a support base, a first gripping mechanism and a second gripping mechanism. The support base is provided with a plurality of loading positions, each of which is used to place one centrifuge tube or one balancing tube. Transferring the centrifuge tube in the first bearing position, or transferring the centrifuge tube in the first bearing position and the balancing tube in the second bearing position, to the receiving position includes the following steps: The centrifuge tube in the first bearing position or the centrifuge tube in the first bearing position and the balancing tube in the second bearing position are transferred to the bearing seat using the first gripping mechanism; The second gripping mechanism is then used to transfer the carrier containing the centrifuge tube or the centrifuge tube and the balancing tube to the receiving position.

12. The sample centrifugation method as described in claim 11, characterized in that, When the first centrifugation is completed, a portion of the liquid in the centrifuge tube or the centrifuge tube and the balancing tube is removed from the centrifuge, and the centrifuge is kept in a balanced state. The process also includes starting the centrifuge for a second centrifugation and removing the centrifuge tube or the balancing tube that has undergone the second centrifugation from the loading position.

13. The sample centrifugation method as described in claim 12, characterized in that, The second centrifugation includes the following step: removing the sample mixture from the centrifuge tube in the centrifuge.

14. The sample centrifugation method as described in claim 12, characterized in that, The centrifuge is removed from the receiving position by the second gripping mechanism, and the centrifuge tube or the centrifuge tube and the balancing tube are removed from the receiving position by the first gripping mechanism; or the centrifuge tube or the centrifuge tube and the balancing tube are removed from the centrifuge by the first gripping mechanism.

15. The sample centrifugation method as described in claim 14, characterized in that, The centrifugation system also includes a tube leveling rack and a test tube rack; The centrifuge tubes are removed and placed in the test tube rack, and the balancing tubes are removed and placed in the balancing tube rack; or... After the centrifuge tubes are removed, they are placed in the test tube rack. After the balancing tubes are removed, they are placed in the test tube rack.

16. A liquid-based integrated machine, characterized in that, The system includes the centrifugation system according to any one of claims 1 to 9; the liquid-based integrated machine performs centrifugation processing according to the sample centrifugation method according to any one of claims 10 to 15.

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